Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 839,124 T→C N544S (AAC→AGC)  b0805 ← putative outer membrane receptor for iron transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913839,1240TC99.6% 814.9 / NA 254N544S (AAC→AGC) b0805putative outer membrane receptor for iron transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (108/145);  minor base A (0/1);  total (108/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

TTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAG  >  NC_000913/839034‑839213
                                                                                          |                                                                                         
 tGGTGTCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGACGAAgctgt                                                                                        >  1:5620221/1‑93 (MQ=255)
   gTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGctgttg                                                                                      <  2:7040122/93‑1 (MQ=255)
    tGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgc                                                                                     >  1:591308/1‑93 (MQ=255)
    tGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgc                                                                                     <  2:9294945/93‑1 (MQ=255)
     gCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgcc                                                                                    >  2:1181669/1‑93 (MQ=255)
     gCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgcc                                                                                    <  1:7606596/93‑1 (MQ=255)
     gCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgcc                                                                                    >  2:2967524/1‑93 (MQ=255)
         aTCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGCTGCCGccc                                                                                >  2:6570581/1‑93 (MQ=255)
          actcGCTGGTGTTGGTTTTTTGCGGCCTAAAATCGGTGTGGTTGGCACTGTTACCGCTGTCAGACTGCGCAAGGGCGAAGCTGTCGCCGCCCg                                                                               >  2:9225867/2‑93 (MQ=255)
             cGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAg                                                                            <  1:3178352/93‑1 (MQ=255)
              gCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAgg                                                                           >  1:199120/1‑93 (MQ=255)
              gCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAgg                                                                           >  2:567864/1‑93 (MQ=255)
                tGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgct                                                                         >  2:1144297/1‑93 (MQ=255)
                tGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgct                                                                         >  1:8497670/1‑93 (MQ=255)
                tGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgct                                                                         >  2:4853639/1‑93 (MQ=255)
                tGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgct                                                                         >  2:7497198/1‑93 (MQ=255)
                 ggTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgctg                                                                        >  2:7967661/1‑93 (MQ=255)
                 ggTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgctg                                                                        >  1:1598342/1‑93 (MQ=255)
                  gtgtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgctgc                                                                       <  1:4248669/93‑1 (MQ=255)
                   tgtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgctgct                                                                      >  2:3549338/1‑93 (MQ=255)
                   tgtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgctgct                                                                      >  1:1835601/1‑93 (MQ=255)
                    gtTGGCTTTTTGCGGTTTAGAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTACCGCCCGGAGGctgctg                                                                     <  2:8957310/93‑1 (MQ=255)
                    gtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGctgctg                                                                     <  2:4047226/93‑1 (MQ=255)
                    gtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGctgctg                                                                     >  1:2763054/1‑93 (MQ=255)
                     ttGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTgg                                                                    <  1:2367051/93‑1 (MQ=255)
                       ggCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGa                                                                  >  2:6360621/1‑93 (MQ=255)
                        gCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGaa                                                                 >  2:4322796/1‑93 (MQ=255)
                        gCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGaa                                                                 <  1:170889/93‑1 (MQ=255)
                          tttttGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAAc                                                               >  1:1653151/1‑93 (MQ=255)
                          tttttGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAAc                                                               >  1:7977714/1‑93 (MQ=255)
                           ttttGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACg                                                              <  2:6068084/93‑1 (MQ=255)
                             ttGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGc                                                            <  2:2396907/93‑1 (MQ=255)
                              tGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCa                                                           <  1:5610235/93‑1 (MQ=255)
                               gCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAt                                                          <  1:5083182/93‑1 (MQ=255)
                               gCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAt                                                          >  2:3334822/1‑93 (MQ=255)
                                cGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATa                                                         >  2:8759153/1‑93 (MQ=255)
                                 ggTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAg                                                        >  2:4011710/1‑93 (MQ=255)
                                 ggTTGAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAg                                                        <  1:7643995/93‑1 (MQ=255)
                                  gTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGt                                                       <  2:9516547/93‑1 (MQ=255)
                                  gTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGt                                                       <  1:4344371/93‑1 (MQ=255)
                                  gTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGt                                                       >  1:9650696/1‑93 (MQ=255)
                                  gTTTAAAATCGGTGCGGTTGACACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCAGGAGGCTGCTGGGAAACGGCATAGt                                                       <  2:2174439/93‑1 (MQ=255)
                                   tttAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCACGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGTCATAGtt                                                      >  1:5471478/1‑93 (MQ=255)
                                   tttAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGtt                                                      >  2:1366110/1‑93 (MQ=255)
                                   tttAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGtt                                                      <  1:4627888/93‑1 (MQ=255)
                                     tAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTaa                                                    <  2:6743957/93‑1 (MQ=255)
                                       aaaTCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAata                                                  <  1:7004232/93‑1 (MQ=255)
                                         aTCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAAtata                                                <  1:9531487/93‑1 (MQ=255)
                                         aTCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAAtata                                                <  2:4965329/93‑1 (MQ=255)
                                         aTCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAAtata                                                <  2:7316474/93‑1 (MQ=255)
                                         aTCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAAtata                                                <  1:4131026/93‑1 (MQ=255)
                                          tCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAg                                               <  1:7839229/93‑1 (MQ=255)
                                             gTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACa                                            <  2:1250953/93‑1 (MQ=255)
                                             gTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACa                                            <  1:4823088/93‑1 (MQ=255)
                                              tGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAt                                           >  2:7895912/1‑93 (MQ=255)
                                               gCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAtt                                          <  2:2205903/93‑1 (MQ=255)
                                                 ggTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGc                                        >  1:9125013/1‑93 (MQ=255)
                                                  gTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGcc                                       >  2:5199439/1‑93 (MQ=255)
                                                  gTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGcc                                       >  2:5077348/1‑93 (MQ=255)
                                                     ggCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGtt                                    >  1:6584640/1‑93 (MQ=255)
                                                      gCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGttt                                   <  1:3255116/93‑1 (MQ=255)
                                                       gacggTTACCGCGTCCAGACTGCGCAAGGGCGAAGCGGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGtttt                                  <  1:900654/89‑1 (MQ=255)
                                                       cACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGtttt                                  >  2:8672067/1‑93 (MQ=255)
                                                       cACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGtttt                                  <  2:4684002/93‑1 (MQ=255)
                                                        aCTGTTAGCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTc                                 <  1:9910071/93‑1 (MQ=255)
                                                        aCTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTc                                 <  1:8684912/93‑1 (MQ=255)
                                                         cTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTcc                                <  2:8790309/93‑1 (MQ=255)
                                                         cTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAACATAGACATTGCCGTTTTcc                                <  1:1300550/93‑1 (MQ=255)
                                                          tGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCg                               <  1:9180932/93‑1 (MQ=255)
                                                           gTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGt                              >  2:5526298/1‑93 (MQ=255)
                                                           gTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGt                              >  2:3110885/1‑93 (MQ=255)
                                                                 gCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTg                        >  2:7555803/1‑93 (MQ=255)
                                                                 gCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTg                        >  1:8887210/1‑93 (MQ=255)
                                                                 gCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTg                        >  1:262233/1‑93 (MQ=255)
                                                                  cTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGa                       <  1:3054191/93‑1 (MQ=255)
                                                                  cTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGa                       <  1:7412393/93‑1 (MQ=255)
                                                                   tGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAt                      >  2:288943/1‑93 (MQ=255)
                                                                   tGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAt                      >  1:8251774/1‑93 (MQ=255)
                                                                   tGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAt                      >  1:6159431/1‑93 (MQ=255)
                                                                    gcgaGACTGCGAAAGGGCGAAGCTGTTGCCGCGCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATa                     <  2:6264784/90‑1 (MQ=255)
                                                                    gCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATa                     >  2:8157886/1‑93 (MQ=255)
                                                                    gCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATa                     <  1:1069551/93‑1 (MQ=255)
                                                                      cAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACa                   <  2:5251532/93‑1 (MQ=255)
                                                                       aGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAg                  <  1:3858203/93‑1 (MQ=255)
                                                                       aGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAg                  >  2:1629300/1‑93 (MQ=255)
                                                                       aGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAg                  >  1:5668746/1‑93 (MQ=255)
                                                                        gACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAgc                 >  2:4380302/1‑93 (MQ=255)
                                                                         aCTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAgcg                <  1:2507418/93‑1 (MQ=255)
                                                                            gcgcAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGccc             <  1:7143028/93‑1 (MQ=255)
                                                                            gcgcAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGccc             <  2:3763700/93‑1 (MQ=255)
                                                                             cgcAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGcccc            <  2:1870412/93‑1 (MQ=255)
                                                                              gcAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCg           >  2:9650265/1‑93 (MQ=255)
                                                                                aaGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGc         >  2:7652284/1‑93 (MQ=255)
                                                                                aaGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGc         >  1:8599901/1‑93 (MQ=255)
                                                                                 aGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCt        >  1:5274183/1‑93 (MQ=255)
                                                                                 aGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCt        >  2:4497868/1‑93 (MQ=255)
                                                                                    gCGAAGCTGTTTCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTc     <  1:2258006/93‑1 (MQ=255)
                                                                                     cGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTcc    <  1:6852626/93‑1 (MQ=255)
                                                                                      gAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCa   <  2:2746365/93‑1 (MQ=255)
                                                                                       aaGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAg  >  2:3222952/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAG  >  NC_000913/839034‑839213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCA  >  NC_000913/839031‑839217
                                                                                             |                                                                                             
CATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGC                                                                                               <  SRR4302540.6423897/94‑1 (MQ=60)
 ATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCT                                                                                              >  SRR4302540.7120791/1‑94 (MQ=60)
 ATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCT                                                                                              >  SRR4302540.9602428/1‑94 (MQ=60)
 ATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCT                                                                                              >  SRR4302540.4530630/1‑94 (MQ=60)
 ATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCT                                                                                              >  SRR4302540.4028930/1‑94 (MQ=60)
 ATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCT                                                                                              >  SRR4302540.9167574/1‑94 (MQ=60)
   TTGGTGTCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGACGAAGCTGT                                                                                            >  SRR4302540.5682056/1‑94 (MQ=60)
   TTGGTGTCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGACGAAGCTGT                                                                                            >  SRR4302540.5682057/1‑94 (MQ=60)
     GGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTG                                                                                          <  SRR4302540.8899667/94‑1 (MQ=60)
      GTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGC                                                                                         >  SRR4302540.597678/1‑94 (MQ=60)
       TGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCC                                                                                        >  SRR4302540.684175/1‑94 (MQ=60)
        GCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCG                                                                                       <  SRR4302540.8772710/94‑1 (MQ=60)
        GCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCG                                                                                       <  SRR4302540.7692074/94‑1 (MQ=60)
        GCCAAGCGCGCTGGTGTTGGCGTTTTGCGGTTTAAAATCGGTGCGGGTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCG                                                                                       <  SRR4302540.7692077/94‑1 (MQ=60)
         CCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGC                                                                                      >  SRR4302540.5195175/1‑94 (MQ=60)
          CAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCC                                                                                     >  SRR4302540.7948548/1‑94 (MQ=60)
            ATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAATGGCGAAGCTGTTGCCGCCCG                                                                                   >  SRR4302540.1173070/1‑94 (MQ=60)
            ATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCG                                                                                   >  SRR4302540.3620019/1‑94 (MQ=60)
               TCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAG                                                                                <  SRR4302540.2583470/94‑1 (MQ=60)
               TCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAG                                                                                <  SRR4302540.3976625/94‑1 (MQ=60)
                CGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGG                                                                               <  SRR4302540.3212892/94‑1 (MQ=60)
                CGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGG                                                                               >  SRR4302540.201089/1‑94 (MQ=60)
                CGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGG                                                                               <  SRR4302540.5748136/94‑1 (MQ=60)
                 GCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCATACTGCGCAAGGGCGAACCTTTTGCCGCCCGGAGGC                                                                              >  SRR4302540.6834076/1‑94 (MQ=60)
                  CTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCT                                                                             >  SRR4302540.8596406/1‑94 (MQ=60)
                  CTGGTGGTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCT                                                                             <  SRR4302540.527837/94‑1 (MQ=60)
                   TGGTGTTGGCTTTTTGCGGTTTACAATCGGTGCGGTTGGCCCTGTTACAGCTGTCAGACTGCGACAGGGCGAAGCTGTTGCCGCCGGGAGGCTG                                                                            >  SRR4302540.1615817/1‑94 (MQ=60)
                   TGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTG                                                                            >  SRR4302540.1615816/1‑94 (MQ=60)
                     GTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCT                                                                          >  SRR4302540.2394608/1‑94 (MQ=60)
                     GTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCT                                                                          <  SRR4302540.4294892/94‑1 (MQ=60)
                     GTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCT                                                                          >  SRR4302540.1855698/1‑94 (MQ=60)
                      TGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTG                                                                         <  SRR4302540.7139843/94‑1 (MQ=60)
                      TGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTG                                                                         >  SRR4302540.7008929/1‑94 (MQ=60)
                      TGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTG                                                                         >  SRR4302540.2792904/1‑94 (MQ=60)
                      TGTTGGCGTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTG                                                                         <  SRR4302540.3137638/94‑1 (MQ=60)
                       GTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGG                                                                        >  SRR4302540.738128/1‑94 (MQ=60)
                       GTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGG                                                                        >  SRR4302540.1813283/1‑94 (MQ=60)
                        TTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGG                                                                       <  SRR4302540.2392545/94‑1 (MQ=60)
                          GGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAA                                                                     <  SRR4302540.4151326/94‑1 (MQ=60)
                          GGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAA                                                                     >  SRR4302540.1958287/1‑94 (MQ=60)
                           GCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAA                                                                    <  SRR4302540.172599/94‑1 (MQ=60)
                            CTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAAC                                                                   <  SRR4302540.8772739/94‑1 (MQ=60)
                            CTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAAC                                                                   >  SRR4302540.1671171/1‑94 (MQ=60)
                            CTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAAC                                                                   >  SRR4302540.8070650/1‑94 (MQ=60)
                            CTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCACTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAAC                                                                   <  SRR4302540.4333846/94‑1 (MQ=60)
                             TTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACG                                                                  <  SRR4302540.8311461/94‑1 (MQ=60)
                                 TGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAT                                                              <  SRR4302540.5671952/94‑1 (MQ=60)
                                 TGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAT                                                              <  SRR4302540.558967/94‑1 (MQ=60)
                                  GCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATA                                                             <  SRR4302540.5640156/94‑1 (MQ=60)
                                  GCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATA                                                             <  SRR4302540.5138141/94‑1 (MQ=60)
                                   CGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAG                                                            <  SRR4302540.2747097/94‑1 (MQ=60)
                                    GGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGT                                                           >  SRR4302540.9764332/1‑94 (MQ=60)
                                    GGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGT                                                           <  SRR4302540.5911628/94‑1 (MQ=60)
                                    GGTTGAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGT                                                           <  SRR4302540.7730029/94‑1 (MQ=60)
                                     GTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCACGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGTCATAGTT                                                          >  SRR4302540.5531705/1‑94 (MQ=60)
                                     GTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTT                                                          <  SRR4302540.3706379/94‑1 (MQ=60)
                                     GTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTT                                                          <  SRR4302540.4391656/94‑1 (MQ=60)
                                      TTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTA                                                         <  SRR4302540.6075217/94‑1 (MQ=60)
                                      TTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTA                                                         <  SRR4302540.7539185/94‑1 (MQ=60)
                                      TTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTA                                                         <  SRR4302540.4677465/94‑1 (MQ=60)
                                      TTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTA                                                         <  SRR4302540.1917169/94‑1 (MQ=60)
                                        TAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAAT                                                       >  SRR4302540.3136164/1‑94 (MQ=60)
                                         AAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATA                                                      <  SRR4302540.421862/94‑1 (MQ=60)
                                         AAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATA                                                      >  SRR4302540.1678540/1‑94 (MQ=60)
                                         AAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATA                                                      <  SRR4302540.1577287/94‑1 (MQ=60)
                                          AAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATAT                                                     <  SRR4302540.7081964/94‑1 (MQ=60)
                                          AAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATAT                                                     <  SRR4302540.1465157/94‑1 (MQ=60)
                                          AAATCGGTGCGCTTGGCACTGTTACCCCTGCCAGACTGCCCAAGCGCGAAGTTGTTGCCGCCCGGAGGCTGATGGGAAACGGCATAGTTAATAT                                                     <  SRR4302540.2356453/94‑1 (MQ=60)
                                           AATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATA                                                    <  SRR4302540.470645/94‑1 (MQ=60)
                                            ATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTTATATAA                                                   >  SRR4302540.5529281/1‑94 (MQ=60)
                                            ATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAG                                                   <  SRR4302540.4175698/94‑1 (MQ=60)
                                            ATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAG                                                   <  SRR4302540.4175697/94‑1 (MQ=60)
                                            ATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAG                                                   <  SRR4302540.9644324/94‑1 (MQ=60)
                                            ATCGGTGCGGTTGGCACTGTTACCGCTCCCAGACTCCCCAAGGGCGAACCTGTTGACGCCCGGAGGCTGCTtggcaactgcatatctagtaaaa                                                   >  SRR4302540.3574900/1‑71 (MQ=46)
                                             TCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGA                                                  <  SRR4302540.7930818/94‑1 (MQ=60)
                                              CGGTGCGGTTGGCTCTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGAC                                                 <  SRR4302540.843656/94‑1 (MQ=60)
                                              CGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGAC                                                 >  SRR4302540.3048921/1‑94 (MQ=60)
                                                GTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAT                                               >  SRR4302540.2456639/1‑94 (MQ=60)
                                                GTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAT                                               <  SRR4302540.4874726/94‑1 (MQ=60)
                                                GTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAT                                               <  SRR4302540.7773417/94‑1 (MQ=60)
                                                GTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTAGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAT                                               <  SRR4302540.4874724/94‑1 (MQ=60)
                                                 TGCGGTTGGAACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGATGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATT                                              <  SRR4302540.8682685/94‑1 (MQ=60)
                                                  GCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTG                                             <  SRR4302540.2563865/94‑1 (MQ=60)
                                                  GCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTG                                             >  SRR4302540.9650832/1‑94 (MQ=60)
                                                   CGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGC                                            <  SRR4302540.6409191/94‑1 (MQ=60)
                                                   CGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGC                                            >  SRR4302540.9234696/1‑94 (MQ=60)
                                                    GGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGATGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCC                                           <  SRR4302540.3007317/94‑1 (MQ=60)
                                                       TGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTT                                        >  SRR4302540.6657576/1‑94 (MQ=60)
                                                         GCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTT                                      <  SRR4302540.3290598/94‑1 (MQ=60)
                                                          gacggttaccgcgtCCAGACTGCGCAAGGGCGAAGCGGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTC                                     <  SRR4302540.910222/80‑1 (MQ=60)
                                                          CACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTC                                     <  SRR4302540.2102464/94‑1 (MQ=60)
                                                           ACTGTTAGCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCC                                    <  SRR4302540.10025824/94‑1 (MQ=60)
                                                           ACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGGAACGGCATAGGTAATATAGACATTGCCGTTTTTC                                    >  SRR4302540.9265026/1‑94 (MQ=60)
                                                           ACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCC                                    >  SRR4302540.9677775/1‑94 (MQ=60)
                                                           ACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCC                                    <  SRR4302540.4981586/94‑1 (MQ=60)
                                                           ACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCC                                    <  SRR4302540.8785761/94‑1 (MQ=60)
                                                           ACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCC                                    <  SRR4302540.7231965/94‑1 (MQ=60)
                                                           ACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCC                                    <  SRR4302540.5687409/94‑1 (MQ=60)
                                                            CTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCG                                   <  SRR4302540.7517644/94‑1 (MQ=60)
                                                            CTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAACATAGACATTGCCGTTTTCCG                                   <  SRR4302540.1314032/94‑1 (MQ=60)
                                                             TGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGT                                  <  SRR4302540.9291045/94‑1 (MQ=60)
                                                                 TCCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGG                              <  SRR4302540.9938740/94‑1 (MQ=60)
                                                                  CCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGGCAAGT                             >  SRR4302540.9005805/1‑94 (MQ=60)
                                                                   CGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTG                            >  SRR4302540.4436328/1‑94 (MQ=60)
                                                                   CGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTG                            >  SRR4302540.8990097/1‑94 (MQ=60)
                                                                   CGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTG                            >  SRR4302540.264793/1‑94 (MQ=60)
                                                                   CGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTG                            <  SRR4302540.928504/94‑1 (MQ=60)
                                                                     CTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAT                          <  SRR4302540.3087703/94‑1 (MQ=60)
                                                                     CTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAT                          <  SRR4302540.5340307/94‑1 (MQ=60)
                                                                     CTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAT                          >  SRR4302540.8347690/1‑94 (MQ=60)
                                                                     CTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAT                          >  SRR4302540.6228059/1‑94 (MQ=60)
                                                                     CTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAT                          <  SRR4302540.7494956/94‑1 (MQ=60)
                                                                      TGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATA                         <  SRR4302540.9374360/94‑1 (MQ=60)
                                                                       GCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATAC                        <  SRR4302540.1080454/94‑1 (MQ=60)
                                                                        CCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACA                       <  SRR4302540.2370172/94‑1 (MQ=60)
                                                                        CCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACA                       <  SRR4302540.5704736/94‑1 (MQ=60)
                                                                         CAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAG                      >  SRR4302540.5731171/1‑94 (MQ=60)
                                                                         CAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAG                      <  SRR4302540.8606770/94‑1 (MQ=60)
                                                                          AGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGC                     <  SRR4302540.3900149/94‑1 (MQ=60)
                                                                            ACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGC                   <  SRR4302540.2534497/94‑1 (MQ=60)
                                                                             CTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCC                  >  SRR4302540.3224020/1‑94 (MQ=60)
                                                                             CTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCC                  >  SRR4302540.4595209/1‑94 (MQ=60)
                                                                              TGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCC                 <  SRR4302540.7793569/94‑1 (MQ=60)
                                                                              TGCGCAAGGGCGAAGCTGTTGCCGCCCGGAAGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCC                 >  SRR4302540.7067742/1‑94 (MQ=60)
                                                                               GCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCC                <  SRR4302540.7222615/94‑1 (MQ=60)
                                                                                CGCAAGGGCGAAGCTGTTTCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCG               <  SRR4302540.8388517/94‑1 (MQ=60)
                                                                                 GCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGCAACGGCATAGATAATATAGACATTGCAGTTTTCCGTCAGGAGATACAGCGCCCCGG              >  SRR4302540.2359835/1‑94 (MQ=60)
                                                                                  CAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGC             >  SRR4302540.9220464/1‑94 (MQ=60)
                                                                                  CAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGC             >  SRR4302540.8699859/1‑94 (MQ=60)
                                                                                   AAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCT            >  SRR4302540.5331764/1‑94 (MQ=60)
                                                                                    AGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTCTTCCGTCAGGTGATACAGCGCCCCGGCTT           >  SRR4302540.1651552/1‑94 (MQ=60)
                                                                                     GGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTT          <  SRR4302540.9957315/94‑1 (MQ=60)
                                                                                      GGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTC         >  SRR4302540.6480849/1‑94 (MQ=60)
                                                                                      GGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTC         >  SRR4302540.9001838/1‑94 (MQ=60)
                                                                                       GCGAAGCTGTTTCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCC        <  SRR4302540.2282242/94‑1 (MQ=60)
                                                                                        CGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCA       <  SRR4302540.6928535/94‑1 (MQ=60)
                                                                                         GAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAG      <  SRR4302540.915824/94‑1 (MQ=60)
                                                                                         GAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAG      >  SRR4302540.8037795/1‑94 (MQ=60)
                                                                                         GAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAG      >  SRR4302540.1762795/1‑94 (MQ=60)
                                                                                             CTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCA  >  SRR4302540.2320086/1‑94 (MQ=60)
                                                                                             |                                                                                             
CATTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCA  >  NC_000913/839031‑839217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑