Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 839,124 T→C N544S (AAC→AGC)  b0805 ← putative outer membrane receptor for iron transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913839,1240TC100.0% 761.0 / NA 237N544S (AAC→AGC) b0805putative outer membrane receptor for iron transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (102/135);  total (102/135)

Only 100 of 251 total aligned reads displayed.

TTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAG  >  NC_000913/839034‑839213
                                                                                          |                                                                                         
ttGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAgctg                                                                                         >  1:4251149/1‑93 (MQ=255)
 tGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAgctgt                                                                                        <  2:1189317/93‑1 (MQ=255)
  ggTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAgctgtt                                                                                       >  1:1546916/1‑93 (MQ=255)
   gTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGctgttg                                                                                      >  2:5610460/1‑93 (MQ=255)
    tGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgc                                                                                     <  2:4326926/93‑1 (MQ=255)
    tGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgc                                                                                     >  2:2768189/1‑93 (MQ=255)
       cAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgccgc                                                                                  <  2:1621389/93‑1 (MQ=255)
       cAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgccgc                                                                                  <  1:5436912/93‑1 (MQ=255)
       cAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgccgc                                                                                  <  1:5100492/93‑1 (MQ=255)
        aaTCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTgccgcc                                                                                 >  2:623789/1‑93 (MQ=255)
         aTCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGccc                                                                                <  1:106901/93‑1 (MQ=255)
         aTCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGccc                                                                                <  2:6181145/93‑1 (MQ=255)
          tctcGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCg                                                                               >  2:5542554/1‑93 (MQ=255)
            tcGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGa                                                                             >  1:6213526/1‑93 (MQ=255)
            tcGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGa                                                                             >  2:4260570/1‑93 (MQ=255)
            tcGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGa                                                                             >  1:3878640/1‑93 (MQ=255)
               cTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgc                                                                          <  1:19942/93‑1 (MQ=255)
               cTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgc                                                                          <  1:4634946/93‑1 (MQ=255)
               cTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgc                                                                          <  1:5545118/93‑1 (MQ=255)
               cTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgc                                                                          <  1:2486434/93‑1 (MQ=255)
               cTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgc                                                                          <  1:4148765/93‑1 (MQ=255)
               cTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgc                                                                          >  2:4482376/1‑93 (MQ=255)
                tGGTGTTGTCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgct                                                                         <  2:3730317/93‑1 (MQ=255)
                  gtgtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgctgc                                                                       <  2:2434112/93‑1 (MQ=255)
                  gtgtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGgctgc                                                                       <  2:736929/93‑1 (MQ=255)
                    gtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGctgctg                                                                     >  2:3024668/1‑93 (MQ=255)
                    gtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGctgctg                                                                     <  1:1662514/93‑1 (MQ=255)
                    gtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGctgctg                                                                     <  2:322034/93‑1 (MQ=255)
                    gtTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGctgctg                                                                     >  2:3957242/1‑93 (MQ=255)
                         cTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGaaa                                                                <  1:2945455/93‑1 (MQ=255)
                          tttttGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGGTGCTGGGaaag                                                               >  2:1247426/1‑92 (MQ=255)
                          tttttGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAAc                                                               >  2:1972694/1‑93 (MQ=255)
                           ttttGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACg                                                              <  2:1061437/93‑1 (MQ=255)
                           ttttGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACg                                                              <  1:4547010/93‑1 (MQ=255)
                               gCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAt                                                          <  1:2316419/93‑1 (MQ=255)
                               gCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAt                                                          <  1:1264378/93‑1 (MQ=255)
                                cGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATa                                                         <  1:1831097/93‑1 (MQ=255)
                                 ggTTTAAAATCGGTGCGGTTGGCGCTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAg                                                        <  1:206156/93‑1 (MQ=255)
                                 ggTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAg                                                        <  1:1956130/93‑1 (MQ=255)
                                  gTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGt                                                       >  1:2706241/1‑93 (MQ=255)
                                  gTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGt                                                       >  1:256791/1‑93 (MQ=255)
                                     tAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTaa                                                    >  2:4626954/1‑93 (MQ=255)
                                     tAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTaa                                                    <  2:2626361/93‑1 (MQ=255)
                                       aaaTCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAata                                                  <  2:2358127/93‑1 (MQ=255)
                                       aaaTCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAata                                                  <  1:3552268/93‑1 (MQ=255)
                                         aTCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAAtata                                                <  2:1390448/93‑1 (MQ=255)
                                         aTCGGTGCGGTTGGCACTGTTACCGCGGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAAtata                                                <  1:2883915/93‑1 (MQ=255)
                                          tCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAg                                               <  1:3967629/93‑1 (MQ=255)
                                           cGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGa                                              <  1:6063680/93‑1 (MQ=255)
                                            ggTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGAc                                             <  1:1585939/93‑1 (MQ=255)
                                             gTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACa                                            <  1:4768020/93‑1 (MQ=255)
                                               gCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAtt                                          >  2:6091385/1‑93 (MQ=255)
                                               gCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAtt                                          <  2:5747735/93‑1 (MQ=255)
                                                 ggTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGc                                        <  1:5581086/93‑1 (MQ=255)
                                                 ggTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGc                                        <  2:1779338/93‑1 (MQ=255)
                                                 ggTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGATAATATAGACATTGc                                        >  1:2795260/1‑93 (MQ=255)
                                                  gTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGcc                                       >  1:4340252/1‑93 (MQ=255)
                                                  gTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGcc                                       <  1:1295382/93‑1 (MQ=255)
                                                     ggCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGtt                                    <  2:711484/93‑1 (MQ=255)
                                                      gCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGttt                                   <  2:2251280/93‑1 (MQ=255)
                                                      gCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGttt                                   <  1:103580/93‑1 (MQ=255)
                                                      gCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGttt                                   <  2:3533199/93‑1 (MQ=255)
                                                      gCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGttt                                   <  2:1651121/93‑1 (MQ=255)
                                                       cACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGtttt                                  <  1:929038/93‑1 (MQ=255)
                                                        aCTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTc                                 >  1:5636071/1‑93 (MQ=255)
                                                        aCTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAAAGGCATAGTTAATATAGACATTGCCGgttgc                                 >  1:3075190/1‑91 (MQ=255)
                                                          tGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCg                               >  2:4904071/1‑93 (MQ=255)
                                                          tGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCg                               >  1:3297179/1‑93 (MQ=255)
                                                           gTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGt                              >  2:3877326/1‑93 (MQ=255)
                                                            ttACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTc                             >  2:497994/1‑93 (MQ=255)
                                                             tACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCa                            <  2:3485726/93‑1 (MQ=255)
                                                               ccGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAgg                          >  1:3854081/1‑93 (MQ=255)
                                                               ccGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAgg                          <  2:3361671/93‑1 (MQ=255)
                                                                cGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGt                         >  2:2525338/1‑93 (MQ=255)
                                                                cGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGt                         <  2:3714489/93‑1 (MQ=255)
                                                                cGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGt                         <  2:1730722/93‑1 (MQ=255)
                                                                 gCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTg                        <  1:3029216/93‑1 (MQ=255)
                                                                  cTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGa                       >  1:714803/1‑93 (MQ=255)
                                                                   tGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAt                      >  1:5320839/1‑93 (MQ=255)
                                                                    gCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATa                     >  2:144170/1‑93 (MQ=255)
                                                                     ccAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATAc                    <  1:593328/93‑1 (MQ=255)
                                                                      cAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACa                   <  2:310833/93‑1 (MQ=255)
                                                                      cAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACa                   >  1:361854/1‑93 (MQ=255)
                                                                       aGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAg                  >  2:2559842/1‑93 (MQ=255)
                                                                        gACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCTGTCAGGTGATACAgc                 <  1:3396159/93‑1 (MQ=255)
                                                                           tGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGcc              <  1:442062/93‑1 (MQ=255)
                                                                           tGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGcc              >  2:2543793/1‑93 (MQ=255)
                                                                           tGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGcc              <  1:944817/93‑1 (MQ=255)
                                                                            gcgcAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAGACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACCGCGccc             >  2:924135/1‑93 (MQ=255)
                                                                             cgcAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGcccc            <  2:516599/93‑1 (MQ=255)
                                                                              gcAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCg           <  1:5374494/93‑1 (MQ=255)
                                                                              gcAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCg           >  1:3637500/1‑93 (MQ=255)
                                                                               cAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCgg          <  2:1354118/93‑1 (MQ=255)
                                                                                 aGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCt        >  2:5925203/1‑93 (MQ=255)
                                                                                 aGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCt        <  2:1015089/93‑1 (MQ=255)
                                                                                 aGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCt        >  1:4005468/1‑93 (MQ=255)
                                                                                  gggCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCtt       >  2:5838967/1‑93 (MQ=255)
                                                                                   ggCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCttt      >  2:5284265/1‑93 (MQ=255)
                                                                                     cGAAGCTGTGGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTcc    <  2:211175/93‑1 (MQ=255)
                                                                                       aaGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAg  >  1:3353562/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAG  >  NC_000913/839034‑839213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCA  >  NC_000913/839033‑839217
                                                                                           |                                                                                             
TTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTG                                                                                             >  SRR4302533.4276795/1‑94 (MQ=60)
  TGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTT                                                                                           >  SRR4302533.1556238/1‑94 (MQ=60)
   GGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTG                                                                                          <  SRR4302533.125040/94‑1 (MQ=60)
    GTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGC                                                                                         <  SRR4302533.428526/94‑1 (MQ=60)
     TGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCC                                                                                        <  SRR4302533.2800090/94‑1 (MQ=60)
     TGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCC                                                                                        <  SRR4302533.1673747/94‑1 (MQ=60)
     GGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCC                                                                                        <  SRR4302533.1860219/94‑1 (MQ=60)
      GCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTCCCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCG                                                                                       <  SRR4302533.994381/94‑1 (MQ=60)
        CAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCC                                                                                     >  SRR4302533.1604851/1‑94 (MQ=60)
        CAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCC                                                                                     <  SRR4302533.5469657/94‑1 (MQ=60)
        CAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCC                                                                                     <  SRR4302533.5131302/94‑1 (MQ=60)
         AATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCC                                                                                    <  SRR4302533.2118295/94‑1 (MQ=60)
         AATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGGTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCC                                                                                    >  SRR4302533.3405424/1‑94 (MQ=60)
          ATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGGGAAGCTGTTGCCGCCCG                                                                                   >  SRR4302533.1850600/1‑94 (MQ=60)
          ATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCG                                                                                   >  SRR4302533.2840222/1‑94 (MQ=60)
          ATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCG                                                                                   <  SRR4302533.107570/94‑1 (MQ=60)
          ATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCG                                                                                   <  SRR4302533.6159592/94‑1 (MQ=60)
          ATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCG                                                                                   <  SRR4302533.5776006/94‑1 (MQ=60)
            CTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGA                                                                                 >  SRR4302533.5627384/1‑94 (MQ=60)
            CTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGA                                                                                 >  SRR4302533.6250785/1‑94 (MQ=60)
            CTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGA                                                                                 >  SRR4302533.3902310/1‑94 (MQ=60)
             TCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAG                                                                                <  SRR4302533.4028338/94‑1 (MQ=60)
             TCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAG                                                                                <  SRR4302533.883876/94‑1 (MQ=60)
              CGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGG                                                                               <  SRR4302533.5786479/94‑1 (MQ=60)
              CGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGG                                                                               <  SRR4302533.1625584/94‑1 (MQ=60)
                CTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCT                                                                             <  SRR4302533.4663032/94‑1 (MQ=60)
                CTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCT                                                                             <  SRR4302533.20079/94‑1 (MQ=60)
                CTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCT                                                                             <  SRR4302533.1180129/94‑1 (MQ=60)
                CTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCT                                                                             <  SRR4302533.4173903/94‑1 (MQ=60)
                CTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCT                                                                             <  SRR4302533.5578609/94‑1 (MQ=60)
                CTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCT                                                                             <  SRR4302533.2501753/94‑1 (MQ=60)
                    TGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTG                                                                         <  SRR4302533.143336/94‑1 (MQ=60)
                    TGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTG                                                                         <  SRR4302533.98335/94‑1 (MQ=60)
                     GTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGG                                                                        <  SRR4302533.1672564/94‑1 (MQ=60)
                      TTGTCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGG                                                                       <  SRR4302533.300014/94‑1 (MQ=60)
                      TTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGG                                                                       >  SRR4302533.3265148/1‑94 (MQ=60)
                         GCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAA                                                                    <  SRR4302533.2578260/94‑1 (MQ=60)
                         GCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAA                                                                    <  SRR4302533.3453618/94‑1 (MQ=60)
                          CTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAAC                                                                   <  SRR4302533.2963470/94‑1 (MQ=60)
                            TTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGG                                                                 <  SRR4302533.4574546/94‑1 (MQ=60)
                            TTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGG                                                                 <  SRR4302533.6096453/94‑1 (MQ=60)
                            TTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGG                                                                 <  SRR4302533.2995793/94‑1 (MQ=60)
                             TTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGC                                                                >  SRR4302533.1451119/1‑94 (MQ=60)
                             TTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGC                                                                <  SRR4302533.1993123/94‑1 (MQ=60)
                               TGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCAT                                                              <  SRR4302533.365541/94‑1 (MQ=60)
                                GCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATA                                                             <  SRR4302533.1271945/94‑1 (MQ=60)
                                GCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATA                                                             <  SRR4302533.2330761/94‑1 (MQ=60)
                                 CGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAG                                                            >  SRR4302533.5349530/1‑94 (MQ=60)
                                 CGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAG                                                            <  SRR4302533.1842274/94‑1 (MQ=60)
                                  GGTTTAAAATCGGTGCGGTTGGCGCTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGT                                                           <  SRR4302533.207464/94‑1 (MQ=60)
                                  GGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGT                                                           >  SRR4302533.2722841/1‑94 (MQ=60)
                                  GGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGT                                                           <  SRR4302533.1968058/94‑1 (MQ=60)
                                  GGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGT                                                           >  SRR4302533.258357/1‑94 (MQ=60)
                                   GTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTT                                                          <  SRR4302533.2903597/94‑1 (MQ=60)
                                   GTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTT                                                          >  SRR4302533.809266/1‑94 (MQ=60)
                                     TTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAA                                                        <  SRR4302533.3718101/94‑1 (MQ=60)
                                       AAAACCGGGGCGTTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTCCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATA                                                      <  SRR4302533.2012328/94‑1 (MQ=60)
                                        AAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATAT                                                     <  SRR4302533.3574148/94‑1 (MQ=60)
                                         AATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATA                                                    >  SRR4302533.5525062/1‑94 (MQ=60)
                                          ATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAG                                                   >  SRR4302533.5136301/1‑94 (MQ=60)
                                          ATCGGTGCGGTTGGCACTGTTACCGCGGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAG                                                   <  SRR4302533.2901651/94‑1 (MQ=60)
                                           TCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGA                                                  <  SRR4302533.3355482/94‑1 (MQ=60)
                                           TCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGA                                                  <  SRR4302533.3991799/94‑1 (MQ=60)
                                            CGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGAC                                                 <  SRR4302533.6100387/94‑1 (MQ=60)
                                             GGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACA                                                <  SRR4302533.1595494/94‑1 (MQ=60)
                                              GTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAT                                               <  SRR4302533.4796923/94‑1 (MQ=60)
                                              GTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAT                                               <  SRR4302533.2852566/94‑1 (MQ=60)
                                              GTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACAT                                               >  SRR4302533.4569645/1‑94 (MQ=60)
                                               TGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATT                                              <  SRR4302533.2651584/94‑1 (MQ=60)
                                               TGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATT                                              <  SRR4302533.4041868/94‑1 (MQ=60)
                                                GCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTTCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTG                                             <  SRR4302533.478268/94‑1 (MQ=60)
                                                 CGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGC                                            >  SRR4302533.5417023/1‑94 (MQ=60)
                                                 CGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGC                                            <  SRR4302533.6243544/94‑1 (MQ=60)
                                                 CGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGATAATATAGACATTGC                                            >  SRR4302533.2812450/1‑94 (MQ=60)
                                                  GGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCC                                           <  SRR4302533.2424052/94‑1 (MQ=60)
                                                  GGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCC                                           >  SRR4302533.4366413/1‑94 (MQ=60)
                                                  GGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCC                                           <  SRR4302533.5614813/94‑1 (MQ=60)
                                                  GGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCCGCCGGAGGCGGCTGGGAAACGGCATAGTTAATATAGACATTGCC                                           >  SRR4302533.3978574/1‑94 (MQ=60)
                                                   GTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCG                                          >  SRR4302533.1067777/1‑94 (MQ=60)
                                                   GTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCG                                          <  SRR4302533.1303180/94‑1 (MQ=60)
                                                     TGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTT                                        <  SRR4302533.3582207/94‑1 (MQ=60)
                                                      GGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTT                                       >  SRR4302533.2492930/1‑94 (MQ=60)
                                                      GGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTT                                       >  SRR4302533.4061242/1‑94 (MQ=60)
                                                       GCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTT                                      <  SRR4302533.950493/94‑1 (MQ=60)
                                                       GCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTT                                      <  SRR4302533.6324248/94‑1 (MQ=60)
                                                       GCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTT                                      <  SRR4302533.104228/94‑1 (MQ=60)
                                                        CACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTC                                     <  SRR4302533.934491/94‑1 (MQ=60)
                                                        CACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTC                                     >  SRR4302533.3094062/1‑94 (MQ=60)
                                                        CACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTC                                     >  SRR4302533.5670153/1‑94 (MQ=60)
                                                        CACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAAAGGCATAGTTAATATAGACATTGCCGgttgc                                     >  SRR4302533.3094063/1‑89 (MQ=60)
                                                         ACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCC                                    <  SRR4302533.2033566/94‑1 (MQ=60)
                                                         ACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCC                                    >  SRR4302533.2609761/1‑94 (MQ=60)
                                                          CTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCG                                   >  SRR4302533.3317527/1‑94 (MQ=60)
                                                           TGTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCAGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGT                                  >  SRR4302533.3407865/1‑94 (MQ=60)
                                                            GTTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTC                                 <  SRR4302533.1571318/94‑1 (MQ=60)
                                                             TTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCA                                <  SRR4302533.4248632/94‑1 (MQ=60)
                                                             TTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCA                                <  SRR4302533.561092/94‑1 (MQ=60)
                                                             TTACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCA                                >  SRR4302533.4533370/1‑94 (MQ=60)
                                                              TACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAG                               >  SRR4302533.381686/1‑94 (MQ=60)
                                                               ACCGCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGG                              >  SRR4302533.3877621/1‑94 (MQ=60)
                                                                  GCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGA                           >  SRR4302533.719021/1‑94 (MQ=60)
                                                                  GCTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGA                           <  SRR4302533.3047752/94‑1 (MQ=60)
                                                                   CTGCCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGAT                          >  SRR4302533.5352727/1‑94 (MQ=60)
                                                                      CCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACA                       <  SRR4302533.596828/94‑1 (MQ=60)
                                                                      CCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACA                       >  SRR4302533.4337567/1‑94 (MQ=60)
                                                                      CCAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACA                       >  SRR4302533.364054/1‑94 (MQ=60)
                                                                       CAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAG                      <  SRR4302533.56150/94‑1 (MQ=60)
                                                                       CAGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAG                      >  SRR4302533.4736792/1‑94 (MQ=60)
                                                                        AGACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGC                     >  SRR4302533.5386137/1‑94 (MQ=60)
                                                                         GACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCTGTCAGGTGATACAGCG                    <  SRR4302533.3417132/94‑1 (MQ=60)
                                                                         GACTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCG                    <  SRR4302533.4619930/94‑1 (MQ=60)
                                                                           CTGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCC                  <  SRR4302533.440560/94‑1 (MQ=60)
                                                                            TGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCC                 <  SRR4302533.950374/94‑1 (MQ=60)
                                                                            TGCGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCC                 <  SRR4302533.444705/94‑1 (MQ=60)
                                                                              CGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCG               >  SRR4302533.3659863/1‑94 (MQ=60)
                                                                              CGCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCG               >  SRR4302533.947932/1‑94 (MQ=60)
                                                                               GCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGG              >  SRR4302533.4898274/1‑94 (MQ=60)
                                                                               GCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGG              <  SRR4302533.5406911/94‑1 (MQ=60)
                                                                               GCAAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGG              <  SRR4302533.5406794/94‑1 (MQ=60)
                                                                                 AAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCT            >  SRR4302533.1288117/1‑94 (MQ=60)
                                                                                 AAGGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCT            >  SRR4302533.4029851/1‑94 (MQ=60)
                                                                                   GGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTT          <  SRR4302533.325271/94‑1 (MQ=60)
                                                                                   GGGCGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTT          <  SRR4302533.3882795/94‑1 (MQ=60)
                                                                                      CGAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCA       >  SRR4302533.3942282/1‑94 (MQ=60)
                                                                                       GAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAG      <  SRR4302533.3589447/94‑1 (MQ=60)
                                                                                       GAAGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAG      >  SRR4302533.3374283/1‑94 (MQ=60)
                                                                                         AGCTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTT    >  SRR4302533.5098687/1‑94 (MQ=60)
                                                                                           CTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATTGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCA  >  SRR4302533.4476038/1‑94 (MQ=60)
                                                                                           |                                                                                             
TTTGGTGCCAATCTCGCTGGTGTTGGCTTTTTGCGGTTTAAAATCGGTGCGGTTGGCACTGTTACCGCTGCCAGACTGCGCAAGGGCGAAGTTGTTGCCGCCCGGAGGCTGCTGGGAAACGGCATAGTTAATATAGACATTGCCGTTTTCCGTCAGGTGATACAGCGCCCCGGCTTTCCAGTTCA  >  NC_000913/839033‑839217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑