Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	857,539	T→C	D249G (GAC→GGC)	ybiU ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	857,539	0	T	C	100.0%	758.2 / NA	234	D249G (GAC→GGC)	ybiU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (119/115); total (119/115)

Only 100 of 251 total aligned reads displayed.

ACGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACG  >  NC_000913/857449‑857628

acgacgTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGccc                                                                                         >  2:7597831/1‑93 (MQ=255)

acgacgTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGccc                                                                                         >  1:9290791/1‑93 (MQ=255)

  gacgTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCAc                                                                                       <  2:2414787/93‑1 (MQ=255)

   acgTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCAcc                                                                                      >  1:2043112/1‑93 (MQ=255)

     gTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCgt                                                                                    >  1:7169227/1‑93 (MQ=255)

       gcagcaGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCgtgt                                                                                  <  2:3524238/93‑1 (MQ=255)

        cagcagCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCGCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTa                                                                                 <  2:708908/93‑1 (MQ=255)

         agccgcCCCTGACCAGGCAGCATATCAGAGAGTGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTAc                                                                                <  1:1848569/93‑1 (MQ=255)

         agcagcCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTAc                                                                                <  2:1230010/93‑1 (MQ=255)

          gcagcCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTAct                                                                               <  1:2254115/93‑1 (MQ=255)

          gcagcCCCTGACCAGGCAGCATATCAGAGACCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTAct                                                                               >  1:8164804/1‑93 (MQ=255)

           cagcCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTActc                                                                              <  2:7126537/93‑1 (MQ=255)

             gcCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCtt                                                                            <  1:4758655/93‑1 (MQ=255)

                 cTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAAc                                                                        >  1:994181/1‑93 (MQ=255)

                 cTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAAc                                                                        >  2:146093/1‑93 (MQ=255)

                 cTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAAc                                                                        >  1:3802507/1‑93 (MQ=255)

                 cTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAAc                                                                        >  2:383458/1‑93 (MQ=255)

                 cTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAAc                                                                        <  2:9251519/93‑1 (MQ=255)

                    aCCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTc                                                                     <  2:239027/93‑1 (MQ=255)

                    aCCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTc                                                                     <  2:4669997/93‑1 (MQ=255)

                     ccAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTcc                                                                    >  1:1634319/1‑93 (MQ=255)

                        ggCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTa                                                                 >  1:549274/1‑93 (MQ=255)

                         gcagcaTATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTAc                                                                >  2:2023103/1‑93 (MQ=255)

                         gcagcaTATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTAc                                                                <  2:5322871/93‑1 (MQ=255)

                         gcagcaTATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTAc                                                                >  1:6385720/1‑93 (MQ=255)

                           agcaTATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGa                                                              >  1:256056/1‑93 (MQ=255)

                           agcaTATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGa                                                              <  2:3093488/93‑1 (MQ=255)

                           agcaTATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGa                                                              <  2:741383/93‑1 (MQ=255)

                            gcaTATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGAt                                                             >  1:10026865/1‑93 (MQ=255)

                             caTATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGAtg                                                            <  1:7575385/93‑1 (MQ=255)

                              atatCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGAtgt                                                           >  2:7895299/1‑93 (MQ=255)

                              atatCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGAtgt                                                           <  2:9055202/93‑1 (MQ=255)

                               tatCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGAtgtg                                                          >  1:1236726/1‑93 (MQ=255)

                               tatCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGAtgtg                                                          >  2:8045527/1‑93 (MQ=255)

                               tatCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGAtgtg                                                          >  2:1795260/1‑93 (MQ=255)

                               tatCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGAtgtg                                                          >  2:8589161/1‑93 (MQ=255)

                               tatCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGAtgtg                                                          >  1:6998130/1‑93 (MQ=255)

                                 tCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGcc                                                        <  2:6648633/93‑1 (MQ=255)

                                  cAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCg                                                       >  1:735465/1‑93 (MQ=255)

                                    gagagCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGct                                                     >  1:7513902/1‑92 (MQ=255)

                                    gagagCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGcc                                                     <  2:6161479/93‑2 (MQ=255)

                                    gagagCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCa                                                     <  2:2551326/93‑1 (MQ=255)

                                     agagCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCAt                                                    >  1:6638188/1‑93 (MQ=255)

                                      gagCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATg                                                   <  2:1556034/93‑1 (MQ=255)

                                      gagCGCTGTCCAGCCATGGAATGTCCGAAGCTCGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATg                                                   <  2:8321261/93‑1 (MQ=255)

                                       agCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTATTCTTCACCTTCCGTCCGATGAGCCGCACGc                                                  >  2:2742964/1‑93 (MQ=255)

                                       agCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGc                                                  <  2:8678816/93‑1 (MQ=255)

                                       agCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGc                                                  >  1:8597909/1‑93 (MQ=255)

                                         cgcTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCa                                                <  2:9472481/93‑1 (MQ=255)

                                           gtGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAgg                                              <  1:8113711/92‑1 (MQ=255)

                                           cTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAgg                                              >  1:1527127/1‑93 (MQ=255)

                                           cTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAgg                                              <  1:8747965/93‑1 (MQ=255)

                                           cTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAgg                                              <  2:5904796/93‑1 (MQ=255)

                                            tGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAggg                                             >  1:5922596/1‑93 (MQ=255)

                                             gTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGa                                            <  2:7497926/93‑1 (MQ=255)

                                                cAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCa                                         <  2:9656736/93‑1 (MQ=255)

                                                 aGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCat                                        <  2:8126621/93‑1 (MQ=255)

                                                 aGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCat                                        <  1:3741349/93‑1 (MQ=255)

                                                 aGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCat                                        >  1:5590365/1‑93 (MQ=255)

                                                 aGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCat                                        >  2:4114726/1‑93 (MQ=255)

                                                  gCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCata                                       <  1:1414197/93‑1 (MQ=255)

                                                   cccTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCatat                                      >  1:2841978/1‑93 (MQ=255)

                                                   cccTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCatat                                      >  1:1874359/1‑93 (MQ=255)

                                                    ccTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATAtt                                     >  2:9186897/1‑93 (MQ=255)

                                                    ccTGGAATGTCCGAAACACGGAACATTTGGGGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCatatg                                     >  2:852739/1‑92 (MQ=255)

                                                     cTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTg                                    >  2:1157355/1‑93 (MQ=255)

                                                     cTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTg                                    >  1:895271/1‑93 (MQ=255)

                                                      tGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTgc                                   <  1:4550216/93‑1 (MQ=255)

                                                      tGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTgc                                   <  1:1180070/93‑1 (MQ=255)

                                                       ggAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTgcg                                  >  1:3023375/1‑93 (MQ=255)

                                                         aaTGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGcc                                <  2:9839188/93‑1 (MQ=255)

                                                          aTGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCa                               >  1:4955471/1‑93 (MQ=255)

                                                          aTGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCa                               >  1:9584352/1‑93 (MQ=255)

                                                           tGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAg                              >  2:1606069/1‑93 (MQ=255)

                                                            gTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGa                             <  2:6996961/93‑1 (MQ=255)

                                                             tCCGAATCTCGGAACATTTGGTGGTATTGCTCACCGTGTTCTCTTCAACTTCCGTACGATGTACCGCATGCCAGGGATCATATTGCGCCatat                            <  2:4822938/93‑1 (MQ=255)

                                                                  aaCACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCa                       <  1:1781735/93‑1 (MQ=255)

                                                                     acGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTa                    >  1:3589368/1‑93 (MQ=255)

                                                                       ggAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTaaa                  >  1:278591/1‑93 (MQ=255)

                                                                       ggAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTaaa                  <  2:7668861/93‑1 (MQ=255)

                                                                        gAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAg                 >  2:8754141/1‑93 (MQ=255)

                                                                        gAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAg                 <  1:1711389/93‑1 (MQ=255)

                                                                        gAACATTTGGTGGTGTTGCCCACCGTGTACTCTCCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAg                 <  1:1941003/93‑1 (MQ=255)

                                                                         aaCATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGa                <  1:6752116/93‑1 (MQ=255)

                                                                         aaCATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGa                >  2:4174653/1‑93 (MQ=255)

                                                                          aCATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGAc               <  2:8642050/93‑1 (MQ=255)

                                                                          aCATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGAc               <  1:4251569/93‑1 (MQ=255)

                                                                            ctttGGGGGTGTTGCCCACCGTGTACTCTTCACCTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGt             <  2:3447244/92‑1 (MQ=255)

                                                                            aTTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGt             <  1:8450396/93‑1 (MQ=255)

                                                                            aTTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGt             >  2:3207631/1‑93 (MQ=255)

                                                                            aTTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGt             >  2:4946206/1‑93 (MQ=255)

                                                                             tttGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGtt            <  1:8620662/93‑1 (MQ=255)

                                                                             gtgggtggtGTTGCCCACCGTGTACTCTTCAACTCCCGTACGATGTGCAGCAGGCCAGGCATCATATTGCGCCAGATTACCATTAAACACGtc            <  2:5752307/90‑2 (MQ=255)

                                                                              ttGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTg           >  1:4658715/1‑93 (MQ=255)

                                                                                ggtggtGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGc         >  1:8501189/1‑93 (MQ=255)

                                                                                 gtggtgTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGTATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCg        >  1:2521023/1‑93 (MQ=255)

                                                                                  tggtgTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGa       >  1:3389673/1‑93 (MQ=255)

                                                                                   ggtgTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGaa      <  2:2102326/93‑1 (MQ=255)

                                                                                    gtgtTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGaaa     >  1:10014141/1‑93 (MQ=255)

                                                                                     tgttgcCCACCGTGTACTCTTCAACTTCAGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGaaaa    <  1:693211/93‑1 (MQ=255)

ACGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACG  >  NC_000913/857449‑857628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CACGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCT  >  NC_000913/857448‑857632

CACGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCC                                                                                             >  SRR4302540.9401822/1‑94 (MQ=60)

  CGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCAC                                                                                           >  SRR4302540.2965276/1‑94 (MQ=60)

   GACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACC                                                                                          >  SRR4302540.2065123/1‑94 (MQ=60)

     CGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGT                                                                                        >  SRR4302540.7249147/1‑94 (MQ=60)

     CGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGT                                                                                        >  SRR4302540.4535924/1‑94 (MQ=60)

      GTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTG                                                                                       >  SRR4302540.17977/1‑94 (MQ=60)

         CAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTAC                                                                                    <  SRR4302540.933717/94‑1 (MQ=60)

         CAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTAC                                                                                    <  SRR4302540.9053090/94‑1 (MQ=60)

          AGCCGCCCCTGACCAGGCAGCATATCAGAGAGTGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACT                                                                                   <  SRR4302540.1868847/94‑1 (MQ=60)

          AGCAGCCCCTGACCAGGCAGCATATCAGAGACCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACT                                                                                   >  SRR4302540.8259612/1‑94 (MQ=60)

           GCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTC                                                                                  <  SRR4302540.2278306/94‑1 (MQ=60)

             AGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTT                                                                                <  SRR4302540.7175605/94‑1 (MQ=60)

              GCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTC                                                                               >  SRR4302540.2587093/1‑94 (MQ=60)

              GCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTC                                                                               <  SRR4302540.4809432/94‑1 (MQ=60)

                 CCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAAC                                                                            >  SRR4302540.1004309/1‑94 (MQ=60)

                 CCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAAC                                                                            >  SRR4302540.3843928/1‑94 (MQ=60)

                  CTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAAGGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACT                                                                           <  SRR4302540.6716226/94‑1 (MQ=60)

                   TGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTT                                                                          >  SRR4302540.8618495/1‑94 (MQ=60)

                    GACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTC                                                                         >  SRR4302540.2032815/1‑94 (MQ=60)

                     ACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCC                                                                        >  SRR4302540.1652198/1‑94 (MQ=60)

                        AGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTA                                                                     >  SRR4302540.555258/1‑94 (MQ=60)

                        AGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTA                                                                     >  SRR4302540.1832251/1‑94 (MQ=60)

                        AGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTA                                                                     >  SRR4302540.8716432/1‑94 (MQ=60)

                        AGGCAGCATATCAGAGAGCACTGTCCAGCCCTGGAATGTCCGAAACACGGTACATTTGGGGGGGTTGCCCACCGTGTACTCTTCAACTTCCGTA                                                                     >  SRR4302540.555259/1‑94 (MQ=60)

                         GGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTAC                                                                    >  SRR4302540.6456807/1‑94 (MQ=60)

                          GCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACG                                                                   <  SRR4302540.8064060/94‑1 (MQ=60)

                          GCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACG                                                                   <  SRR4302540.10137693/94‑1 (MQ=60)

                           CAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGA                                                                  >  SRR4302540.258539/1‑94 (MQ=60)

                            AGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGAT                                                                 >  SRR4302540.10143531/1‑94 (MQ=60)

                              CATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGT                                                               <  SRR4302540.7660382/94‑1 (MQ=60)

                              CATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGT                                                               <  SRR4302540.2629033/94‑1 (MQ=60)

                              CATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGT                                                               >  SRR4302540.8219493/1‑94 (MQ=60)

                               ATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTG                                                              >  SRR4302540.1249354/1‑94 (MQ=60)

                               ATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTG                                                              >  SRR4302540.7075797/1‑94 (MQ=60)

                               ATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTG                                                              >  SRR4302540.7075798/1‑94 (MQ=60)

                               ATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTG                                                              >  SRR4302540.3434870/1‑94 (MQ=60)

                               ATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTG                                                              >  SRR4302540.8079709/1‑94 (MQ=60)

                                 ATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCC                                                            >  SRR4302540.9385668/1‑94 (MQ=60)

                                 ATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCC                                                            >  SRR4302540.4637358/1‑94 (MQ=60)

                                 ATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCC                                                            <  SRR4302540.2104121/94‑1 (MQ=60)

                                  TCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCG                                                           >  SRR4302540.743325/1‑94 (MQ=60)

                                    AGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCT                                                         >  SRR4302540.7597906/1‑94 (MQ=60)

                                    AGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCA                                                         <  SRR4302540.8808432/94‑1 (MQ=60)

                                    AGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCA                                                         >  SRR4302540.1013157/1‑94 (MQ=60)

                                     GAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCAT                                                        >  SRR4302540.6711649/1‑94 (MQ=60)

                                     GAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCAT                                                        <  SRR4302540.2463453/94‑1 (MQ=60)

                                      AGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATG                                                       >  SRR4302540.4757501/1‑94 (MQ=60)

                                       GAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGC                                                      >  SRR4302540.8697845/1‑94 (MQ=60)

                                        AGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCC                                                     <  SRR4302540.7214030/94‑1 (MQ=60)

                                          CGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAG                                                   >  SRR4302540.6911356/1‑94 (MQ=60)

                                          CGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAG                                                   >  SRR4302540.6911355/1‑94 (MQ=60)

                                          CGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAG                                                   >  SRR4302540.1689783/1‑94 (MQ=60)

                                           GCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGG                                                  >  SRR4302540.1544041/1‑94 (MQ=60)

                                            GTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGG                                                 <  SRR4302540.8207885/94‑1 (MQ=60)

                                            CTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGG                                                 <  SRR4302540.1932483/94‑1 (MQ=60)

                                            CTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGG                                                 >  SRR4302540.5988033/1‑94 (MQ=60)

                                            CTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGG                                                 <  SRR4302540.8849481/94‑1 (MQ=60)

                                            CTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGG                                                 <  SRR4302540.8849480/94‑1 (MQ=60)

                                             TGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGT                                                >  SRR4302540.1415866/1‑94 (MQ=60)

                                              GTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGAT                                               >  SRR4302540.158050/1‑94 (MQ=60)

                                               TCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATC                                              <  SRR4302540.2691057/94‑1 (MQ=60)

                                                CCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCA                                             <  SRR4302540.1597533/94‑1 (MQ=60)

                                                 CAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCAT                                            >  SRR4302540.5651883/1‑94 (MQ=60)

                                                 CAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCAT                                            >  SRR4302540.432027/1‑94 (MQ=60)

                                                  AGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATA                                           <  SRR4302540.2336689/94‑1 (MQ=60)

                                                  AGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATA                                           <  SRR4302540.3782172/94‑1 (MQ=60)

                                                   GCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATAT                                          <  SRR4302540.1429457/94‑1 (MQ=60)

                                                   GCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATAT                                          >  SRR4302540.1894889/1‑94 (MQ=60)

                                                   GCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATAT                                          >  SRR4302540.2872731/1‑94 (MQ=60)

                                                    CCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATT                                         >  SRR4302540.6385831/1‑94 (MQ=60)

                                                     CCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTG                                        >  SRR4302540.8278778/1‑94 (MQ=60)

                                                     CCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTG                                        >  SRR4302540.904785/1‑94 (MQ=60)

                                                     CCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTG                                        >  SRR4302540.8364699/1‑94 (MQ=60)

                                                      CTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGC                                       >  SRR4302540.3053408/1‑94 (MQ=60)

                                                      CTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGC                                       >  SRR4302540.4964271/1‑94 (MQ=60)

                                                       TGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCG                                      <  SRR4302540.1192259/94‑1 (MQ=60)

                                                       TGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCG                                      >  SRR4302540.3056593/1‑94 (MQ=60)

                                                       TGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCG                                      <  SRR4302540.8583548/94‑1 (MQ=60)

                                                       TGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCG                                      <  SRR4302540.4599430/94‑1 (MQ=60)

                                                         GAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCC                                    >  SRR4302540.7516135/1‑94 (MQ=60)

                                                         GAATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCC                                    >  SRR4302540.7516134/1‑94 (MQ=60)

                                                          AATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCA                                   >  SRR4302540.5009017/1‑94 (MQ=60)

                                                          AATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCA                                   >  SRR4302540.9697507/1‑94 (MQ=60)

                                                          AATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCA                                   >  SRR4302540.4195880/1‑94 (MQ=60)

                                                          AATGTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCACGCCAGGGATCATATTGCGCCA                                   >  SRR4302540.9428767/1‑94 (MQ=60)

                                                             GTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGAT                                >  SRR4302540.383845/1‑94 (MQ=60)

                                                             GTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGAT                                >  SRR4302540.577658/1‑94 (MQ=60)

                                                             GTCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGAT                                >  SRR4302540.383846/1‑94 (MQ=60)

                                                              TCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATT                               <  SRR4302540.9817008/94‑1 (MQ=60)

                                                              TCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATT                               <  SRR4302540.10150444/94‑1 (MQ=60)

                                                              TCCGAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATT                               >  SRR4302540.3690726/1‑94 (MQ=60)

                                                                 GAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCC                            >  SRR4302540.1877828/1‑94 (MQ=60)

                                                                 GAAACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCC                            >  SRR4302540.1397739/1‑94 (MQ=60)

                                                                 GAAACACGGAACATTTGGTGGGGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCC                            >  SRR4302540.1877829/1‑94 (MQ=60)

                                                                   AACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCAT                          <  SRR4302540.1801089/94‑1 (MQ=60)

                                                                    ACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATT                         <  SRR4302540.1741903/94‑1 (MQ=60)

                                                                    ACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATT                         <  SRR4302540.9786772/94‑1 (MQ=60)

                                                                    ACACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATT                         >  SRR4302540.8654660/1‑94 (MQ=60)

                                                                     CACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTA                        <  SRR4302540.3546743/94‑1 (MQ=60)

                                                                     CACGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTA                        >  SRR4302540.3628578/1‑94 (MQ=60)

                                                                       CGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAA                      >  SRR4302540.281325/1‑94 (MQ=60)

                                                                       CGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAA                      <  SRR4302540.5893516/94‑1 (MQ=60)

                                                                       CGGAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGAT‑ATATTGCGCCAGATTGCCATTAAAG                     >  SRR4302540.1483692/1‑94 (MQ=60)

                                                                         GAACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGA                    <  SRR4302540.1729876/94‑1 (MQ=60)

                                                                         GAACATTTGGTGGTGTTGCCCACCGTGTACTCTCCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGA                    <  SRR4302540.1962138/94‑1 (MQ=60)

                                                                          AACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGAC                   <  SRR4302540.6826724/94‑1 (MQ=60)

                                                                           ACATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACG                  <  SRR4302540.4297823/94‑1 (MQ=60)

                                                                             ATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTT                >  SRR4302540.197942/1‑94 (MQ=60)

                                                                             ATTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTT                <  SRR4302540.8548503/94‑1 (MQ=60)

                                                                              TTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTG               >  SRR4302540.4708578/1‑94 (MQ=60)

                                                                              TTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTG               <  SRR4302540.8720799/94‑1 (MQ=60)

                                                                              TTTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTG               <  SRR4302540.7330417/94‑1 (MQ=60)

                                                                               TTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGG              <  SRR4302540.7648717/94‑1 (MQ=60)

                                                                               TTGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGG              <  SRR4302540.3192858/94‑1 (MQ=60)

                                                                                TGGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGC             >  SRR4302540.8599969/1‑94 (MQ=60)

                                                                                 GGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGTATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCG            >  SRR4302540.2548246/1‑94 (MQ=60)

                                                                                 GGTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCG            <  SRR4302540.1200284/94‑1 (MQ=60)

                                                                                  GTGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGA           >  SRR4302540.3426655/1‑94 (MQ=60)

                                                                                   TGGTGTTGCCCACCGTGTCCTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAA          <  SRR4302540.6551369/94‑1 (MQ=60)

                                                                                   TGGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAA          <  SRR4302540.6551373/94‑1 (MQ=60)

                                                                                    GGTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAA         >  SRR4302540.10130725/1‑94 (MQ=60)

                                                                                     GTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAA        <  SRR4302540.4777416/94‑1 (MQ=60)

                                                                                     GTGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAA        <  SRR4302540.2857129/94‑1 (MQ=60)

                                                                                      TGTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAAC       <  SRR4302540.5111910/94‑1 (MQ=60)

                                                                                      TGTTGCCCACCGTGTACTCTTCAACTTCAGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAAC       <  SRR4302540.700642/94‑1 (MQ=60)

                                                                                       GTTGCCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACG      >  SRR4302540.5154500/1‑94 (MQ=60)

                                                                                           CCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCT  <  SRR4302540.774998/94‑1 (MQ=60)

                                                                                           CCCACCATGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCT  <  SRR4302540.3462634/94‑1 (MQ=60)

CACGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCT  >  NC_000913/857448‑857632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`