Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	862,272	T→C	T164A (ACT→GCT)	ybiY ←	putative pyruvate formate‑lyase activating enzyme 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	862,272	0	T	C	99.6%	796.4 / NA	247	T164A (ACT→GCT)	ybiY	putative pyruvate formate‑lyase activating enzyme 2
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (125/121); total (126/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

CGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGC  >  NC_000913/862182‑862361

 gtgtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATg                                                                                        <  1:9927508/93‑1 (MQ=255)

 gtgtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATg                                                                                        <  2:616788/93‑1 (MQ=255)

  tgtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGa                                                                                       >  2:2265425/1‑93 (MQ=255)

  tgtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGa                                                                                       >  1:1981624/1‑93 (MQ=255)

  tgtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGa                                                                                       >  1:2479071/1‑93 (MQ=255)

  tgtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGa                                                                                       <  2:658398/93‑1 (MQ=255)

   gtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      <  2:1289112/93‑1 (MQ=255)

   gtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      <  2:4697098/93‑1 (MQ=255)

   gtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      <  2:5503096/93‑1 (MQ=255)

     tttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtg                                                                                    >  1:609930/1‑93 (MQ=255)

     tttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtg                                                                                    >  1:1950582/1‑93 (MQ=255)

      ttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgc                                                                                   <  2:8366256/93‑1 (MQ=255)

      ttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgc                                                                                   <  1:1523612/93‑1 (MQ=255)

       tAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgcc                                                                                  >  2:6072800/1‑93 (MQ=255)

       tAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgcc                                                                                  <  1:6662081/93‑1 (MQ=255)

         aaTCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTGCCGCAGCATGAAtacctg                                                                                >  1:4441230/1‑93 (MQ=255)

            cGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATgcctgcct                                                                             <  2:9345195/93‑1 (MQ=255)

             ggCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTc                                                                            >  2:309362/1‑93 (MQ=255)

              gCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCg                                                                           >  1:4026357/1‑93 (MQ=255)

               gcaGAAACAAATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGt                                                                          <  1:5721991/91‑1 (MQ=255)

               cAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGt                                                                          <  2:444090/93‑1 (MQ=255)

                 aGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtgg                                                                        >  1:1239910/1‑93 (MQ=255)

                 aGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtgg                                                                        >  1:8085908/1‑93 (MQ=255)

                  gAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATTCCTGCCTCGtggc                                                                       >  2:8911970/1‑93 (MQ=255)

                  gAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggc                                                                       >  2:2112889/1‑93 (MQ=255)

                  gAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggc                                                                       >  2:9755125/1‑93 (MQ=255)

                  gAAACAGATCGATATAGGGCAGAGAAGCGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggc                                                                       <  2:3519489/93‑1 (MQ=255)

                    aaCAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggctg                                                                     <  2:4672099/93‑1 (MQ=255)

                      cAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggctggt                                                                   <  2:1857436/93‑2 (MQ=255)

                       aGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTggctggct                                                                  <  2:5890096/93‑1 (MQ=255)

                        gATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCtt                                                                 >  1:6795007/1‑93 (MQ=255)

                         aTCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTg                                                                >  2:7820303/1‑93 (MQ=255)

                         aTCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTg                                                                <  1:9806481/93‑1 (MQ=255)

                         aTCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCCTg                                                                >  2:2028235/1‑93 (MQ=255)

                         aTCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCCCAGCATGAATGCCTGCCTCGTGGCTGGCTTg                                                                >  1:9154213/1‑93 (MQ=255)

                           cGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCa                                                              <  2:6699714/93‑1 (MQ=255)

                           cGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCa                                                              >  1:9562407/1‑93 (MQ=255)

                            gATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAg                                                             <  2:4099882/93‑1 (MQ=255)

                            gATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCATGccg                                                             >  1:5494732/1‑91 (MQ=255)

                            gATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGATTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGcct                                                             >  1:2896999/1‑91 (MQ=255)

                             atatAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGt                                                            >  2:4033171/1‑93 (MQ=255)

                              tataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTTCAGTa                                                           >  2:2403831/1‑93 (MQ=255)

                              tataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTa                                                           <  1:6720827/93‑1 (MQ=255)

                              aagaGGGCAGACAAGGGGCGATATATTTCCACGGCACATGCAGACGGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTa                                                           <  2:4928567/90‑1 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          >  1:4762866/1‑93 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          <  2:7169196/93‑1 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          <  1:5304840/93‑1 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          <  1:333013/93‑1 (MQ=255)

                                taGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgc                                                         >  2:5628334/1‑93 (MQ=255)

                                  gggCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc                                                       >  2:8277761/1‑93 (MQ=255)

                                  gggCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc                                                       <  2:6958698/93‑1 (MQ=255)

                                   ggCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgcc                                                      <  1:7610461/93‑1 (MQ=255)

                                    gCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgcca                                                     >  1:4181196/1‑93 (MQ=255)

                                    gCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgcca                                                     >  2:8797213/1‑93 (MQ=255)

                                    gCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTATACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgcca                                                     <  1:7880751/93‑1 (MQ=255)

                                     cAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccat                                                    <  2:1675735/93‑1 (MQ=255)

                                     cAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccat                                                    <  2:799150/93‑1 (MQ=255)

                                      agagAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatc                                                   <  2:298976/93‑1 (MQ=255)

                                        agaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTGGCAGTAGcgccatcgc                                                 <  2:7953187/93‑1 (MQ=255)

                                         gaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgcc                                                >  1:3866419/1‑93 (MQ=255)

                                          aaGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgcca                                               <  2:1397018/93‑1 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              >  1:648113/1‑93 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              <  1:7549702/93‑1 (MQ=255)

                                                  atatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc                                       >  1:6965581/1‑93 (MQ=255)

                                                      atTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCa                                   >  2:7591598/1‑93 (MQ=255)

                                                      atTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCa                                   <  2:10035105/93‑1 (MQ=255)

                                                        ttCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATa                                 <  2:872234/93‑1 (MQ=255)

                                                        ttCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATa                                 <  1:7687194/93‑1 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               <  2:3756467/93‑1 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               >  2:1808500/1‑93 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               <  1:9387763/93‑1 (MQ=255)

                                                              ggCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGc                           <  1:3122769/93‑1 (MQ=255)

                                                               gCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGcc                          >  2:5096661/1‑92 (MQ=255)

                                                               gCACATGCACACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGcc                          <  2:898185/93‑2 (MQ=255)

                                                                cacaTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGccc                         <  2:5475215/93‑3 (MQ=255)

                                                                 acaTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCca                        >  1:5148479/1‑93 (MQ=255)

                                                                   aTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacc                      <  1:1547239/93‑1 (MQ=255)

                                                                   aTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCcacc                      <  2:2547379/93‑1 (MQ=255)

                                                                       aGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCAccc                  >  2:2862234/1‑93 (MQ=255)

                                                                        gACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCg                 >  2:4793022/1‑93 (MQ=255)

                                                                         aCAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGa                >  1:269142/1‑93 (MQ=255)

                                                                          cAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGaa               >  1:7946119/1‑93 (MQ=255)

                                                                           aGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGaaa              >  2:765971/1‑93 (MQ=255)

                                                                            ggTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAg             >  2:5352614/1‑93 (MQ=255)

                                                                            ggTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAg             >  1:2940584/1‑93 (MQ=255)

                                                                             gTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGc            >  1:3195999/1‑93 (MQ=255)

                                                                                tCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGtt         <  2:7514245/93‑1 (MQ=255)

                                                                                tCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGtt         <  2:917170/93‑1 (MQ=255)

                                                                                 cTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTa        >  2:6780594/1‑93 (MQ=255)

                                                                                  tACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTaa       >  1:1741805/1‑93 (MQ=255)

                                                                                  tACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTaa       <  1:9999614/93‑1 (MQ=255)

                                                                                    ccGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAc     <  2:1150500/93‑1 (MQ=255)

                                                                                    ccGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAAc     >  1:7457097/1‑93 (MQ=255)

                                                                                    ccGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAAc     <  2:7884529/93‑1 (MQ=255)

                                                                                    ccGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAAc     <  1:8322653/93‑1 (MQ=255)

                                                                                     cGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAAcc    <  1:8083087/93‑1 (MQ=255)

                                                                                       cAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAccgc  <  1:10010060/93‑1 (MQ=255)

                                                                                       cAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAccgc  <  1:1846393/93‑1 (MQ=255)

                                                                                       cAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAccgc  <  1:8345709/93‑1 (MQ=255)

                                                                                       cAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAccgc  <  1:5989995/93‑1 (MQ=255)

CGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGC  >  NC_000913/862182‑862361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAACGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGC  >  NC_000913/862179‑862364

CAACGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGC                                                                                              <  SRR4302540.7287981/94‑1 (MQ=60)

CAACGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGC                                                                                              <  SRR4302540.4565064/94‑1 (MQ=60)

 AACGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCA                                                                                             >  SRR4302540.7477703/1‑94 (MQ=60)

 AACGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCA                                                                                             >  SRR4302540.622877/1‑94 (MQ=60)

  ACGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCAT                                                                                            <  SRR4302540.8590996/94‑1 (MQ=60)

   CGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATG                                                                                           <  SRR4302540.10094710/94‑1 (MQ=60)

   CGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATG                                                                                           >  SRR4302540.7049202/1‑94 (MQ=60)

    GTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGA                                                                                          >  SRR4302540.3936840/1‑94 (MQ=60)

    GTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGA                                                                                          >  SRR4302540.2505773/1‑94 (MQ=60)

    GTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGA                                                                                          >  SRR4302540.2003115/1‑94 (MQ=60)

    GTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGA                                                                                          <  SRR4302540.10043406/94‑1 (MQ=60)

      GTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAT                                                                                        <  SRR4302540.5199845/94‑1 (MQ=60)

      GTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAT                                                                                        <  SRR4302540.7339898/94‑1 (MQ=60)

      GTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAT                                                                                        <  SRR4302540.2960544/94‑1 (MQ=60)

      GTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAT                                                                                        <  SRR4302540.9709267/94‑1 (MQ=60)

      GTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAT                                                                                        <  SRR4302540.607130/94‑1 (MQ=60)

       TTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATG                                                                                       >  SRR4302540.616450/1‑94 (MQ=60)

       TTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATG                                                                                       >  SRR4302540.1971804/1‑94 (MQ=60)

         TTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCC                                                                                     >  SRR4302540.7610024/1‑94 (MQ=60)

         TTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCC                                                                                     <  SRR4302540.1540475/94‑1 (MQ=60)

          TAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCT                                                                                    <  SRR4302540.6735732/94‑1 (MQ=60)

           AAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTGCCGCAGCATGAATACCTG                                                                                   >  SRR4302540.4489359/1‑94 (MQ=60)

           AAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTG                                                                                   <  SRR4302540.3173438/94‑1 (MQ=60)

            AATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGC                                                                                  <  SRR4302540.5889081/94‑1 (MQ=60)

                GGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCG                                                                              >  SRR4302540.4069774/1‑94 (MQ=60)

                 GCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGT                                                                             >  SRR4302540.2147086/1‑94 (MQ=60)

                  gcAGAAACAAATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTG                                                                            <  SRR4302540.5784979/92‑1 (MQ=60)

                  CAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTG                                                                            <  SRR4302540.4222679/94‑1 (MQ=60)

                   AAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGG                                                                           >  SRR4302540.8315687/1‑94 (MQ=60)

                   AAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGG                                                                           >  SRR4302540.8179760/1‑94 (MQ=60)

                   AAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGG                                                                           >  SRR4302540.1252571/1‑94 (MQ=60)

                   AAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGG                                                                           >  SRR4302540.6686175/1‑94 (MQ=60)

                   AAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGG                                                                           >  SRR4302540.3710961/1‑94 (MQ=60)

                   AAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGG                                                                           >  SRR4302540.8179759/1‑94 (MQ=60)

                    AGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGC                                                                          <  SRR4302540.1590651/94‑1 (MQ=60)

                          AGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTT                                                                    <  SRR4302540.6850313/94‑1 (MQ=60)

                          AGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTT                                                                    >  SRR4302540.6870158/1‑94 (MQ=60)

                           GATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCCCAGCATGAATGCCTGCCTCGTGGCTGGCTTG                                                                   >  SRR4302540.9264117/1‑94 (MQ=60)

                            ATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGC                                                                  <  SRR4302540.9921267/94‑1 (MQ=60)

                             TCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCA                                                                 >  SRR4302540.532851/1‑94 (MQ=60)

                             TCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCA                                                                 >  SRR4302540.9675447/1‑94 (MQ=60)

                              CGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCATGCCG                                                                >  SRR4302540.5555226/1‑94 (MQ=60)

                              CGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGATTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCct                                                                >  SRR4302540.2928594/1‑92 (MQ=60)

                                ATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTA                                                              <  SRR4302540.1044877/94‑1 (MQ=60)

                                ATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTA                                                              >  SRR4302540.1736933/1‑94 (MQ=60)

                                 TATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAG                                                             <  SRR4302540.4386828/94‑1 (MQ=60)

                                 TATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAG                                                             >  SRR4302540.4813703/1‑94 (MQ=60)

                                 TATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAG                                                             <  SRR4302540.155950/94‑1 (MQ=60)

                                 TATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAG                                                             <  SRR4302540.6795001/94‑1 (MQ=60)

                                  ATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGC                                                            <  SRR4302540.4461654/94‑1 (MQ=60)

                                  ATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGC                                                            >  SRR4302540.7985181/1‑94 (MQ=60)

                                  ATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGC                                                            <  SRR4302540.336364/94‑1 (MQ=60)

                                  ATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGC                                                            <  SRR4302540.5362885/94‑1 (MQ=60)

                                   TAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCG                                                           <  SRR4302540.1380206/94‑1 (MQ=60)

                                    AGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGC                                                          >  SRR4302540.5455126/1‑94 (MQ=60)

                                      GGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCA                                                        >  SRR4302540.4226616/1‑94 (MQ=60)

                                      GGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCA                                                        <  SRR4302540.7695996/94‑1 (MQ=60)

                                       GCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTATACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCAT                                                       <  SRR4302540.7972778/94‑1 (MQ=60)

                                        CAGAGAAGGGGCGATATATTTCCGCGGCAGATGCAGACAGGGTTCGACAGCAGCATGAATGCCTGtgtcgtgtgccgcttgccgcatggccatc                                                      >  SRR4302540.6854005/1‑65 (MQ=34)

                                        CAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATC                                                      >  SRR4302540.6854317/1‑94 (MQ=60)

                                        CAGAGAAGGGACGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATC                                                      >  SRR4302540.7663436/1‑94 (MQ=60)

                                         AGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCG                                                     >  SRR4302540.1957855/1‑94 (MQ=60)

                                         AGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCG                                                     >  SRR4302540.462453/1‑94 (MQ=60)

                                          GAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGC                                                    <  SRR4302540.6740810/94‑1 (MQ=60)

                                           AGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCC                                                   >  SRR4302540.1783200/1‑94 (MQ=60)

                                           AGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCC                                                   >  SRR4302540.3908448/1‑94 (MQ=60)

                                             AAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCAT                                                 >  SRR4302540.655015/1‑94 (MQ=60)

                                              AGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATT                                                <  SRR4302540.7634327/94‑1 (MQ=60)

                                              AGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATT                                                >  SRR4302540.398121/1‑94 (MQ=60)

                                                GGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTC                                              >  SRR4302540.2688823/1‑94 (MQ=60)

                                                 GGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCC                                             <  SRR4302540.2748454/94‑1 (MQ=60)

                                                    GATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGC                                          <  SRR4302540.1983555/94‑1 (MQ=60)

                                                    GATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGC                                          >  SRR4302540.7043034/1‑94 (MQ=60)

                                                     ATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCT                                         >  SRR4302540.7729904/1‑94 (MQ=60)

                                                      TATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTG                                        >  SRR4302540.6503483/1‑94 (MQ=60)

                                                      TATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTG                                        >  SRR4302540.6946525/1‑94 (MQ=60)

                                                       ATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGC                                       >  SRR4302540.6064397/1‑94 (MQ=60)

                                                         ATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCCGCTGCAT                                     >  SRR4302540.9291339/1‑94 (MQ=60)

                                                          TTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATA                                    <  SRR4302540.5751954/94‑1 (MQ=60)

                                                           TTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAA                                   <  SRR4302540.7774439/94‑1 (MQ=60)

                                                             CCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAG                                 <  SRR4302540.9499492/94‑1 (MQ=60)

                                                              CACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGG                                <  SRR4302540.7947519/94‑1 (MQ=60)

                                                              CACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGG                                >  SRR4302540.8056763/1‑94 (MQ=60)

                                                              CACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGG                                <  SRR4302540.2035127/94‑1 (MQ=60)

                                                               ACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGG                               <  SRR4302540.4906840/94‑1 (MQ=60)

                                                                 GGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCC                             <  SRR4302540.3156881/94‑1 (MQ=60)

                                                                   CACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCC                           >  SRR4302540.1944656/1‑94 (MQ=60)

                                                                   CACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCA                           >  SRR4302540.3287937/1‑94 (MQ=60)

                                                                   CACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCA                           <  SRR4302540.8897119/94‑1 (MQ=60)

                                                                   CACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCA                           >  SRR4302540.5204287/1‑94 (MQ=60)

                                                                    ACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCAC                          <  SRR4302540.2855279/94‑1 (MQ=60)

                                                                     CATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACC                         <  SRR4302540.956902/94‑1 (MQ=60)

                                                                      ATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCA                        <  SRR4302540.1564306/94‑1 (MQ=60)

                                                                         CAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCC                     >  SRR4302540.3147023/1‑94 (MQ=60)

                                                                          AGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCA                    >  SRR4302540.8313287/1‑94 (MQ=60)

                                                                           GACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGA                   >  SRR4302540.271793/1‑94 (MQ=60)

                                                                            ACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAA                  >  SRR4302540.8038740/1‑94 (MQ=60)

                                                                              AGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAG                >  SRR4302540.2972876/1‑94 (MQ=60)

                                                                              AGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAG                >  SRR4302540.7667363/1‑94 (MQ=60)

                                                                               GGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGC               >  SRR4302540.6652879/1‑94 (MQ=60)

                                                                               GGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGC               >  SRR4302540.3230708/1‑94 (MQ=60)

                                                                                 TTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGT             <  SRR4302540.9998059/94‑1 (MQ=60)

                                                                                 TTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGT             <  SRR4302540.786289/94‑1 (MQ=60)

                                                                                   TCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTA           >  SRR4302540.8172317/1‑94 (MQ=60)

                                                                                   TCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTA           <  SRR4302540.1344426/94‑1 (MQ=60)

                                                                                   TCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTA           >  SRR4302540.9146152/1‑94 (MQ=60)

                                                                                    CTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAA          >  SRR4302540.1760650/1‑94 (MQ=60)

                                                                                    CTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAA          >  SRR4302540.9576956/1‑94 (MQ=60)

                                                                                     TACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGTATAAAGGGCCCACCACCCGAAAGCGTTAAA         <  SRR4302540.10116117/94‑1 (MQ=60)

                                                                                     TACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAA         <  SRR4302540.5510636/94‑1 (MQ=60)

                                                                                     TACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAA         <  SRR4302540.10116116/94‑1 (MQ=60)

                                                                                     TACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAA         >  SRR4302540.3314307/1‑94 (MQ=60)

                                                                                     TACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAA         <  SRR4302540.9981362/94‑1 (MQ=60)

                                                                                      ACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAAC        >  SRR4302540.7540237/1‑94 (MQ=60)

                                                                                       CCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAACC       <  SRR4302540.8419251/94‑1 (MQ=60)

                                                                                        CGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCG      >  SRR4302540.589236/1‑94 (MQ=60)

                                                                                        CGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAACCG      <  SRR4302540.8176900/94‑1 (MQ=60)

                                                                                          CAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCC    <  SRR4302540.10126621/94‑1 (MQ=60)

                                                                                          CAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCC    <  SRR4302540.6056351/94‑1 (MQ=60)

                                                                                          CAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCC    <  SRR4302540.8442573/94‑1 (MQ=60)

                                                                                          CAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCC    <  SRR4302540.8442572/94‑1 (MQ=60)

                                                                                          CAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCC    <  SRR4302540.1866626/94‑1 (MQ=60)

                                                                                          CAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAACCGCC    <  SRR4302540.3099574/94‑1 (MQ=60)

                                                                                            GCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAACCGCCGC  >  SRR4302540.8155288/1‑94 (MQ=60)

                                                                                            GCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAACCGCCGC  <  SRR4302540.4467126/94‑1 (MQ=60)

CAACGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGCCGC  >  NC_000913/862179‑862364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`