Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	866,644	A→G	N285S (AAT→AGT)	ybiK →	putative asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	866,644	0	A	G	98.8%	756.5 / ‑1.4	244	N285S (AAT→AGT)	ybiK	putative asparaginase
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (130/110); total (131/113)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

Only 100 of 259 total aligned reads displayed.

AGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAG  >  NC_000913/866553‑866733

 gTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTg                                                                                         <  2:9719679/93‑1 (MQ=255)

    tcGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTcg                                                                                      <  1:428920/93‑1 (MQ=255)

     cgcgGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTcgc                                                                                     <  2:1066326/93‑1 (MQ=255)

     cgcgGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTcgc                                                                                     <  1:7431176/93‑1 (MQ=255)

       cgGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCgcgc                                                                                   >  2:3829174/1‑93 (MQ=255)

        ggAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCt                                                                                  >  1:2723835/1‑93 (MQ=255)

         gAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTa                                                                                 <  1:1403912/93‑1 (MQ=255)

           aGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTAcc                                                                               <  1:1134337/93‑1 (MQ=255)

           aGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTAcc                                                                               <  1:3364531/93‑1 (MQ=255)

           aGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTAcc                                                                               <  1:2140381/93‑1 (MQ=255)

           aGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGAGTCGCGCTAcc                                                                               >  2:1729972/1‑93 (MQ=255)

            gCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCg                                                                              <  2:9388988/93‑1 (MQ=255)

               tGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGttt                                                                           <  2:2899221/93‑1 (MQ=255)

               tGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGttt                                                                           <  2:7581135/93‑1 (MQ=255)

                gCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTa                                                                          >  1:7152807/1‑93 (MQ=255)

                gCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTa                                                                          >  2:935424/1‑93 (MQ=255)

                gCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTa                                                                          >  2:5314118/1‑93 (MQ=255)

                 cGAGCGGGTAGTAATGGAAAACCTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTaa                                                                         <  1:4955127/93‑1 (MQ=255)

                   aGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAaca                                                                       <  1:10010513/93‑1 (MQ=255)

                   aGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAaca                                                                       >  1:6446814/1‑93 (MQ=255)

                   aGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGACGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAaca                                                                       <  1:9669934/93‑1 (MQ=255)

                    gCGGGTAGTAATAGAGAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAacac                                                                      <  2:8953674/93‑1 (MQ=255)

                      gggTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCg                                                                    >  1:2032716/1‑93 (MQ=255)

                      gggTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCg                                                                    >  2:4239352/1‑93 (MQ=255)

                       ggTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGa                                                                   <  2:10033262/93‑1 (MQ=255)

                        gtagtaATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGaa                                                                  >  1:6431241/1‑93 (MQ=255)

                        gtagtaATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGaa                                                                  >  2:9696732/1‑93 (MQ=255)

                            taATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGaa                                                              >  1:5295550/1‑93 (MQ=255)

                               tGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGt                                                           <  2:8353222/93‑1 (MQ=255)

                               tGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGt                                                           >  1:3842752/1‑93 (MQ=255)

                                ggAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTa                                                          <  1:5164593/93‑1 (MQ=255)

                                ggAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTa                                                          <  2:1261176/93‑1 (MQ=255)

                                 gAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGGTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTAt                                                         <  1:1817849/93‑1 (MQ=255)

                                 gAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTAt                                                         >  1:6825210/1‑93 (MQ=255)

                                 gAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAAGGTAt                                                         >  2:1293492/1‑93 (MQ=255)

                                  aaaaaCTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATc                                                        <  2:3239255/93‑1 (MQ=255)

                                  aaaaaCTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATc                                                        <  1:957054/93‑1 (MQ=255)

                                    aaaCTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATcgc                                                      >  1:3251908/1‑93 (MQ=255)

                                    aaaCTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATcgc                                                      >  1:8753164/1‑93 (MQ=255)

                                        tCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCt                                                  <  2:7766218/93‑1 (MQ=255)

                                        tCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCt                                                  <  2:3106676/93‑1 (MQ=255)

                                        tCCCTGCGCTTGGCGGTAGCGGTGGCTTAATAGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATGGCGccg                                                  >  2:5373508/1‑92 (MQ=255)

                                         cccTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGGGCCTg                                                 >  2:4050540/1‑93 (MQ=255)

                                         cccTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTg                                                 <  2:3724739/93‑1 (MQ=255)

                                         cccTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTAGCGCTACCGTTTAACACCGAAGGAATGTATCACGCCTg                                                 >  2:5691828/1‑93 (MQ=255)

                                          ccTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTgg                                                >  1:7920629/1‑93 (MQ=255)

                                          ccTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTgg                                                >  1:8588881/1‑93 (MQ=255)

                                          ccTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTgg                                                <  1:3558109/93‑1 (MQ=255)

                                             gcgcTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGc                                             <  2:5299718/93‑1 (MQ=255)

                                              ggcTGGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGGCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCt                                            <  1:3959018/92‑1 (MQ=255)

                                               gcTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTa                                           <  1:606785/93‑1 (MQ=255)

                                                cTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTAc                                          <  1:9330251/93‑1 (MQ=255)

                                                 ttGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACg                                         <  2:7893143/93‑1 (MQ=255)

                                                  tGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGc                                        >  2:4393494/1‑93 (MQ=255)

                                                  tGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGc                                        >  1:5755582/1‑93 (MQ=255)

                                                  tGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGc                                        >  2:6312445/1‑93 (MQ=255)

                                                  tGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGc                                        >  1:6582067/1‑93 (MQ=255)

                                                     cGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAgg                                     >  2:6518734/1‑93 (MQ=255)

                                                      ggTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGc                                    <  2:6780314/93‑1 (MQ=255)

                                                      ggTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGc                                    >  2:2509075/1‑93 (MQ=255)

                                                       gTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAggag                                   >  2:7065051/1‑91 (MQ=255)

                                                       gTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCg                                   >  2:5501684/1‑93 (MQ=255)

                                                       gTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGCGCTACGCAGGCg                                   >  2:3463697/1‑93 (MQ=255)

                                                        gaGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGa                                  <  1:8092736/92‑1 (MQ=255)

                                                          gCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATa                                <  2:9013039/93‑1 (MQ=255)

                                                          gCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATa                                <  1:5027693/93‑1 (MQ=255)

                                                          gCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGC‑GGCGATAc                               >  2:6900814/1‑93 (MQ=255)

                                                             gTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGc                             >  1:9043034/1‑93 (MQ=255)

                                                             gTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGc                             >  1:7841390/1‑93 (MQ=255)

                                                               ggCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCa                           <  2:6184660/93‑1 (MQ=255)

                                                               ggCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCa                           >  1:7344836/1‑93 (MQ=255)

                                                               ggCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCa                           >  1:6143293/1‑93 (MQ=255)

                                                                gCTTAATCTCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCaa                          <  1:4544840/93‑1 (MQ=255)

                                                                gCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCaa                          >  2:1974169/1‑93 (MQ=255)

                                                                 cTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAac                         >  2:4639163/1‑93 (MQ=255)

                                                                 cTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAac                         >  1:5519199/1‑93 (MQ=255)

                                                                 cTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAac                         <  1:4652748/93‑1 (MQ=255)

                                                                  ttAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAacc                        >  1:7495397/1‑93 (MQ=255)

                                                                   tAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAaccc                       >  2:2022051/1‑92 (MQ=255)

                                                                   tAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAacca                       >  1:4161643/1‑93 (MQ=255)

                                                                    aaTCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAaccac                      >  1:4840926/1‑93 (MQ=255)

                                                                    aaTCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAaccac                      <  2:5634447/93‑1 (MQ=255)

                                                                     aTCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAaccacc                     >  2:8090238/1‑93 (MQ=255)

                                                                      tCGCTATCGACGATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCg                    <  2:1898847/93‑1 (MQ=255)

                                                                         cTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTa                 >  2:1360411/1‑93 (MQ=255)

                                                                          tATCGACCATGAAGGGAGTGTCGCGCTACCTTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTAt                <  2:7334879/93‑1 (MQ=255)

                                                                          tATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTAt                >  2:4880594/1‑93 (MQ=255)

                                                                                ccATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCg          >  2:8494003/1‑93 (MQ=255)

                                                                                  aTGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTg        >  1:6971323/1‑93 (MQ=255)

                                                                                  aTGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTg        >  1:1964199/1‑93 (MQ=255)

                                                                                   tGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGa       >  1:8481332/1‑93 (MQ=255)

                                                                                   tGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGa       <  2:8825945/93‑1 (MQ=255)

                                                                                    gAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGaa      >  1:1995093/1‑93 (MQ=255)

                                                                                    gAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGaa      >  1:2247707/1‑93 (MQ=255)

                                                                                      aGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGaaaa    <  1:2658657/93‑1 (MQ=255)

                                                                                      aGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGaaaa    <  2:9358843/93‑1 (MQ=255)

                                                                                      aGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGaaaa    <  1:7160797/93‑1 (MQ=255)

                                                                                       gggAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGaaaaa   >  1:8299109/1‑93 (MQ=255)

                                                                                       gggAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGaaaaa   <  2:4294611/93‑1 (MQ=255)

                                                                                        ggAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGACAAAg  >  1:9929403/1‑93 (MQ=255)

AGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAG  >  NC_000913/866553‑866733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGA  >  NC_000913/866551‑866737

TAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAG                                                                                               <  SRR4302540.1546137/94‑1 (MQ=60)

   GTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGT                                                                                            <  SRR4302540.7709227/94‑1 (MQ=60)

   GTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGT                                                                                            >  SRR4302540.5282127/1‑94 (MQ=60)

   GTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGT                                                                                            <  SRR4302540.7576139/94‑1 (MQ=60)

    TCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTC                                                                                           <  SRR4302540.9055634/94‑1 (MQ=60)

     CTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCG                                                                                          <  SRR4302540.10095987/94‑1 (MQ=60)

      TCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGC                                                                                         <  SRR4302540.433429/94‑1 (MQ=60)

       CGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCG                                                                                        >  SRR4302540.8939805/1‑94 (MQ=60)

       CGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCG                                                                                        <  SRR4302540.7513957/94‑1 (MQ=60)

       CGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCG                                                                                        <  SRR4302540.7513958/94‑1 (MQ=60)

       CGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGAGTCGCG                                                                                        >  SRR4302540.9729444/1‑94 (MQ=60)

         CGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCT                                                                                      >  SRR4302540.5496822/1‑94 (MQ=60)

         CGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCT                                                                                      >  SRR4302540.5916817/1‑94 (MQ=60)

         CGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCT                                                                                      >  SRR4302540.237103/1‑94 (MQ=60)

         CGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCT                                                                                      >  SRR4302540.2753205/1‑94 (MQ=60)

         CGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCT                                                                                      >  SRR4302540.2207902/1‑94 (MQ=60)

         CGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCT                                                                                      <  SRR4302540.8317713/94‑1 (MQ=60)

         CGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCT                                                                                      >  SRR4302540.8028713/1‑94 (MQ=60)

           GAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTAC                                                                                    <  SRR4302540.1419029/94‑1 (MQ=60)

             AGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCG                                                                                  <  SRR4302540.1146160/94‑1 (MQ=60)

             AGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCG                                                                                  <  SRR4302540.2163385/94‑1 (MQ=60)

             AGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCG                                                                                  <  SRR4302540.3401344/94‑1 (MQ=60)

               CCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTT                                                                                >  SRR4302540.6928870/1‑94 (MQ=60)

                 TGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTA                                                                              >  SRR4302540.2987556/1‑94 (MQ=60)

                 TGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTA                                                                              >  SRR4302540.7232519/1‑94 (MQ=60)

                   CGAGCGGGTAGTGATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAAC                                                                            <  SRR4302540.6355524/94‑1 (MQ=60)

                   CGAGCGGGTAGTAATGGAAAACCTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAAC                                                                            <  SRR4302540.5008669/94‑1 (MQ=60)

                   CGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAAC                                                                            <  SRR4302540.5008670/94‑1 (MQ=60)

                    GAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACA                                                                           >  SRR4302540.6518340/1‑94 (MQ=60)

                    GAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACA                                                                           >  SRR4302540.6518578/1‑94 (MQ=60)

                     AGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACAC                                                                          <  SRR4302540.10127077/94‑1 (MQ=60)

                     AGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGACGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACAC                                                                          <  SRR4302540.9783738/94‑1 (MQ=60)

                       CGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCG                                                                        >  SRR4302540.19320/1‑94 (MQ=60)

                       CGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCG                                                                        >  SRR4302540.2054645/1‑94 (MQ=60)

                         GGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAA                                                                      <  SRR4302540.5752829/94‑1 (MQ=60)

                         GGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAA                                                                      >  SRR4302540.6502824/1‑94 (MQ=60)

                          GTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAG                                                                     >  SRR4302540.1472984/1‑94 (MQ=60)

                          GTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAG                                                                     <  SRR4302540.3450402/94‑1 (MQ=60)

                            AGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGA                                                                   >  SRR4302540.1185340/1‑94 (MQ=60)

                             GTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAA                                                                  >  SRR4302540.5353469/1‑94 (MQ=60)

                             GTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAA                                                                  >  SRR4302540.5353468/1‑94 (MQ=60)

                             GTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGGGTCGGGCTACCGGTTAACACCGCAGGAA                                                                  >  SRR4302540.2349046/1‑94 (MQ=60)

                              TAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAAT                                                                 <  SRR4302540.5109775/94‑1 (MQ=60)

                              TAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGGGTCGCGCTACCGTTTAACACCGAAGGAAT                                                                 >  SRR4302540.3392821/1‑94 (MQ=60)

                               AATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATG                                                                <  SRR4302540.1377601/94‑1 (MQ=60)

                               AATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATG                                                                <  SRR4302540.7780263/94‑1 (MQ=60)

                                ATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGT                                                               >  SRR4302540.3884545/1‑94 (MQ=60)

                                  GGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTAT                                                             >  SRR4302540.6900772/1‑94 (MQ=60)

                                  GGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTAT                                                             <  SRR4302540.5220755/94‑1 (MQ=60)

                                  GGAAAAACTCCCTGCGCTGGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGGGTCCCGCTACCCTTTAACACCGAAGGAAGGTAT                                                             >  SRR4302540.6900767/1‑94 (MQ=60)

                                   GAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGGTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATC                                                            <  SRR4302540.1837709/94‑1 (MQ=60)

                                   GAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATC                                                            <  SRR4302540.9235587/94‑1 (MQ=60)

                                    AAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCG                                                           <  SRR4302540.966974/94‑1 (MQ=60)

                                     AAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGC                                                          >  SRR4302540.6158085/1‑94 (MQ=60)

                                     AAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGC                                                          >  SRR4302540.8854711/1‑94 (MQ=60)

                                     AAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGC                                                          >  SRR4302540.3287342/1‑94 (MQ=60)

                                      AAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCG                                                         <  SRR4302540.1064485/94‑1 (MQ=60)

                                        ACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCC                                                       >  SRR4302540.6162056/1‑94 (MQ=60)

                                          TCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTG                                                     <  SRR4302540.723126/94‑1 (MQ=60)

                                           CCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGG                                                    >  SRR4302540.8012945/1‑94 (MQ=60)

                                           CCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGG                                                    >  SRR4302540.8688725/1‑94 (MQ=60)

                                           CCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGG                                                    <  SRR4302540.1858555/94‑1 (MQ=60)

                                            CCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGG                                                   <  SRR4302540.4662849/94‑1 (MQ=60)

                                            CCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGG                                                   <  SRR4302540.3596984/94‑1 (MQ=60)

                                            CCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGG                                                   >  SRR4302540.7437357/1‑94 (MQ=60)

                                             CTGCGCTTGGCGGTAGCGGGGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGG                                                  <  SRR4302540.7231828/94‑1 (MQ=60)

                                               GCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCT                                                <  SRR4302540.9466583/94‑1 (MQ=60)

                                               GCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCT                                                >  SRR4302540.2147636/1‑94 (MQ=60)

                                                ggctgGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGGCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTA                                               <  SRR4302540.4001724/89‑1 (MQ=60)

                                                 GCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTAC                                              <  SRR4302540.613271/94‑1 (MQ=60)

                                                 GCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTAC                                              >  SRR4302540.4269312/1‑94 (MQ=60)

                                                  CTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACG                                             <  SRR4302540.9441504/94‑1 (MQ=60)

                                                   TTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGC                                            >  SRR4302540.5819114/1‑94 (MQ=60)

                                                   TTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGC                                            >  SRR4302540.6654977/1‑94 (MQ=60)

                                                   TTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGC                                            >  SRR4302540.4592727/1‑94 (MQ=60)

                                                   TTGGCGGTAGCGGTGGCTTAATCGCAATCGACCATGAAGGGATAGTCGAGCTACCGTTTAAAACCGAAGGAATGTATCGTGCCTGGGTCTACCC                                            >  SRR4302540.2050608/1‑94 (MQ=60)

                                                     GGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAG                                          <  SRR4302540.8668288/94‑1 (MQ=60)

                                                       CGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGTC                                        <  SRR4302540.8134056/94‑1 (MQ=60)

                                                        GGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCG                                       <  SRR4302540.203048/94‑1 (MQ=60)

                                                        GGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCG                                       >  SRR4302540.4925333/1‑94 (MQ=60)

                                                        GGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCG                                       >  SRR4302540.4925331/1‑94 (MQ=60)

                                                        GGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCG                                       <  SRR4302540.7981559/94‑1 (MQ=60)

                                                        GGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCG                                       >  SRR4302540.428383/1‑94 (MQ=60)

                                                        GGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCG                                       >  SRR4302540.2066992/1‑94 (MQ=60)

                                                          GAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGAT                                     <  SRR4302540.8186673/94‑1 (MQ=60)

                                                           AGCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATA                                    >  SRR4302540.4144181/1‑94 (MQ=60)

                                                            GCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATAC                                   <  SRR4302540.7243536/94‑1 (MQ=60)

                                                            GCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATAC                                   <  SRR4302540.5082106/94‑1 (MQ=60)

                                                            GCGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATAC                                   <  SRR4302540.8821450/94‑1 (MQ=60)

                                                            GCGGTGGCTTAATCGCTATCGACCATGAAGAGACTGTCGCGCTACCGTTTAACACCGAAGGAATGTGTCGCGCCTGGGGCTACGCAGGCGATAC                                   <  SRR4302540.841989/94‑1 (MQ=60)

                                                             CGGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACG                                  >  SRR4302540.10004021/1‑94 (MQ=60)

                                                              GGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGC                                 >  SRR4302540.7933018/1‑94 (MQ=60)

                                                              GGTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGC                                 >  SRR4302540.9151582/1‑94 (MQ=60)

                                                               GTGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCC                                >  SRR4302540.9863557/1‑94 (MQ=60)

                                                                TGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCA                               >  SRR4302540.7426786/1‑94 (MQ=60)

                                                                TGGCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCA                               >  SRR4302540.6211713/1‑94 (MQ=60)

                                                                  GCTTAATCTCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAAC                             <  SRR4302540.4594022/94‑1 (MQ=60)

                                                                  GCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAAC                             >  SRR4302540.5579986/1‑94 (MQ=60)

                                                                  GCTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAAC                             >  SRR4302540.5579987/1‑94 (MQ=60)

                                                                   CTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACC                            >  SRR4302540.7579115/1‑94 (MQ=60)

                                                                   CTTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACC                            <  SRR4302540.4702537/94‑1 (MQ=60)

                                                                    TTAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCA                           >  SRR4302540.4206751/1‑94 (MQ=60)

                                                                     TAATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCAC                          >  SRR4302540.4892781/1‑94 (MQ=60)

                                                                     TAATCGCTATCGACCATGAAGGGAGTGTAGCGCTACCGTTTAACACCGAAGGAATGTATAGCGCCTGGGGCTGCGCAGGCGAgcagccaacaac                          >  SRR4302540.4892780/1‑82 (MQ=60)

                                                                      AATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACC                         <  SRR4302540.6679403/94‑1 (MQ=60)

                                                                      AATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACC                         >  SRR4302540.664005/1‑94 (MQ=60)

                                                                       ATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCG                        <  SRR4302540.5086560/94‑1 (MQ=60)

                                                                       ATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCG                        >  SRR4302540.6423822/1‑94 (MQ=60)

                                                                       ATCGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCG                        <  SRR4302540.4705495/94‑1 (MQ=60)

                                                                        TCGCTATCGACCATGAAGGGAGTGTCGCGCTTCCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGG                       <  SRR4302540.1999124/94‑1 (MQ=60)

                                                                         CGCTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGT                      >  SRR4302540.3637/1‑94 (MQ=60)

                                                                          tgTATCGGCCATGAAGGAAGTGTCGCGCTACCGTTTAGCACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTA                     <  SRR4302540.6987779/92‑1 (MQ=60)

                                                                           CTATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTAT                    <  SRR4302540.8521327/94‑1 (MQ=60)

                                                                            TATCGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATC                   >  SRR4302540.3938423/1‑94 (MQ=60)

                                                                               CGACCATGAAGTCAGTGTCGCGCTGCCGTTTAACACCGAAGGAATGTATCGCACCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTAC                <  SRR4302540.690295/94‑1 (MQ=60)

                                                                               CGACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTAC                <  SRR4302540.690294/94‑1 (MQ=60)

                                                                                GACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACC               >  SRR4302540.2732104/1‑94 (MQ=60)

                                                                                GACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACC               >  SRR4302540.2810220/1‑94 (MQ=60)

                                                                                GACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACC               >  SRR4302540.8788638/1‑94 (MQ=60)

                                                                                 ACCATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCG              <  SRR4302540.3754196/94‑1 (MQ=60)

                                                                                   CATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTG            >  SRR4302540.1985534/1‑94 (MQ=60)

                                                                                   CATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTG            <  SRR4302540.7189965/94‑1 (MQ=60)

                                                                                   CATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTG            >  SRR4302540.7048807/1‑94 (MQ=60)

                                                                                   CATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTG            <  SRR4302540.6634095/94‑1 (MQ=60)

                                                                                    ATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGA           >  SRR4302540.7338221/1‑94 (MQ=60)

                                                                                    ATGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGA           >  SRR4302540.8579852/1‑94 (MQ=60)

                                                                                     TGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAA          >  SRR4302540.2271850/1‑94 (MQ=60)

                                                                                     TGAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAA          >  SRR4302540.2016714/1‑94 (MQ=60)

                                                                                      GAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAA         >  SRR4302540.3336787/1‑94 (MQ=60)

                                                                                      GAAGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAA         <  SRR4302540.9490822/94‑1 (MQ=60)

                                                                                        AGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAA       >  SRR4302540.8395474/1‑94 (MQ=60)

                                                                                        AGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAA       <  SRR4302540.4690901/94‑1 (MQ=60)

                                                                                        AGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAA       <  SRR4302540.2687507/94‑1 (MQ=60)

                                                                                        AGGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAA       <  SRR4302540.7240615/94‑1 (MQ=60)

                                                                                         GGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGACAAAG      >  SRR4302540.10045311/1‑94 (MQ=60)

                                                                                         GGGAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAG      <  SRR4302540.4023654/94‑1 (MQ=60)

                                                                                           GAGTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGG    <  SRR4302540.8999339/94‑1 (MQ=60)

                                                                                             GTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGA  <  SRR4302540.2207797/94‑1 (MQ=60)

                                                                                             GTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGA  >  SRR4302540.2808179/1‑94 (MQ=60)

                                                                                             GTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGA  >  SRR4302540.8170108/1‑94 (MQ=60)

                                                                                             GTGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGA  >  SRR4302540.7270833/1‑94 (MQ=60)

                                                                                             GTGTCGCGCTACCGTTTAACACCGAAGGAATGCATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGA  >  SRR4302540.1020553/1‑94 (MQ=60)

TAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGA  >  NC_000913/866551‑866737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`