Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 875,981 C→T V426V (GTG→GTA)  yliG ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913875,9810CT98.9% 968.7 / ‑2.6 267V426V (GTG→GTA) yliGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (123/141);  total (124/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 290 total aligned reads displayed.

TATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTCACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACC  >  NC_000913/875891‑876070
                                                                                          |                                                                                         
tattCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTtac                                                                                         <  2:1504927/93‑1 (MQ=255)
tattCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTtac                                                                                         >  2:785592/1‑93 (MQ=255)
 attCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTtaca                                                                                        <  1:8156866/93‑1 (MQ=255)
  ttCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTacac                                                                                       <  1:1911414/93‑1 (MQ=255)
  ttCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTCTacac                                                                                       <  1:8280738/93‑1 (MQ=255)
    cGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGc                                                                                     <  1:4152292/93‑1 (MQ=255)
     gCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCa                                                                                    >  2:5481583/1‑93 (MQ=255)
     gCAGGCCGAACAAATGATTTTGCCCGTCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCa                                                                                    <  2:5077029/93‑1 (MQ=255)
     ccAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCa                                                                                    <  2:7329540/92‑1 (MQ=255)
      cAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAg                                                                                   >  1:1214587/1‑93 (MQ=255)
      cAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAg                                                                                   <  1:2876784/93‑1 (MQ=255)
       aGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAgg                                                                                  >  2:8593406/1‑93 (MQ=255)
        ggCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGa                                                                                 <  2:5226675/93‑1 (MQ=255)
         gCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGat                                                                                <  1:6035796/93‑1 (MQ=255)
         gCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGat                                                                                <  1:5718844/93‑1 (MQ=255)
            ggACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATc                                                                             >  2:66891/1‑93 (MQ=255)
            ggACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATc                                                                             <  1:7114818/93‑1 (MQ=255)
              aCAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCAc                                                                           >  2:8198397/1‑93 (MQ=255)
                aaaTGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCAccc                                                                         <  2:22027/93‑1 (MQ=255)
                 aaTGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCg                                                                        >  1:8923516/1‑93 (MQ=255)
                 aaTGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCg                                                                        <  1:1599804/93‑1 (MQ=255)
                 aaTGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCg                                                                        >  1:4069200/1‑93 (MQ=255)
                  aTGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCgg                                                                       <  2:5345974/93‑1 (MQ=255)
                  aTGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCgg                                                                       <  2:131905/93‑1 (MQ=255)
                     aTTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCtt                                                                    >  1:6243531/1‑93 (MQ=255)
                         tGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACg                                                                >  2:1807057/1‑93 (MQ=255)
                          gCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGt                                                               <  1:9899205/93‑1 (MQ=255)
                             cGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTgg                                                            <  2:4390633/93‑1 (MQ=255)
                              ggCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGt                                                           <  2:4997588/93‑1 (MQ=255)
                              ggCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGt                                                           >  2:8527285/1‑93 (MQ=255)
                              ggCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGt                                                           >  1:3622948/1‑93 (MQ=255)
                               gCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGtt                                                          <  1:9247384/93‑1 (MQ=255)
                               gCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGtt                                                          >  1:2206539/1‑93 (MQ=255)
                               gCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACAAGCAGGATATCACCCGGCTTAACGTTGGtt                                                          >  2:5703138/1‑93 (MQ=255)
                               gCCTGAATTAATTAAACCCGGCTACCACACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGtt                                                          >  2:1837583/1‑93 (MQ=255)
                                ccTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGttt                                                         <  1:8438944/93‑1 (MQ=255)
                                ccTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGttt                                                         >  2:3877850/1‑93 (MQ=255)
                                  tGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCa                                                       >  2:481024/1‑93 (MQ=255)
                                  tGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCa                                                       >  1:6390614/1‑93 (MQ=255)
                                  tGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCa                                                       <  2:7259292/93‑1 (MQ=255)
                                   gAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCAc                                                      >  2:45598/1‑93 (MQ=255)
                                    aattaattAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTTGTTTCAcc                                                     >  2:1978840/1‑93 (MQ=255)
                                     attaattaAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCa                                                    <  1:8862553/93‑1 (MQ=255)
                                      ttaattaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCAt                                                   <  2:2954960/93‑1 (MQ=255)
                                        aattaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTCACGTTGGTTTCACCATTg                                                 >  1:4685580/1‑93 (MQ=255)
                                        aattaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTg                                                 <  2:8358007/93‑1 (MQ=255)
                                        aattaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTg                                                 >  1:3672353/1‑93 (MQ=255)
                                         attaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTga                                                <  1:2268923/93‑1 (MQ=255)
                                           taaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTgaga                                              <  1:1116448/93‑1 (MQ=255)
                                             atcacGGTTACCTCACAAATCGTACTCATCGGCGTGCTCGACTATAACACGCAGGATATCACCCGGCTTAACGTAGGTTTAACCATTGAGATa                                            <  2:598759/89‑1 (MQ=255)
                                             accccGGCTCCCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGTCTTAACGTTGGTGTCCCCATTGAGATa                                            <  2:1059642/91‑1 (MQ=255)
                                              aCCCGGCTACCCCACAAATCGTACTCATCGGCGTTCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATaa                                           <  1:8790066/93‑1 (MQ=255)
                                               cccGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATaaa                                          <  1:4229652/93‑1 (MQ=255)
                                               cccGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATaaa                                          <  2:9493997/93‑1 (MQ=255)
                                               cccGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATaaa                                          <  2:9379653/93‑1 (MQ=255)
                                                ccGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAAc                                         <  2:3243354/93‑1 (MQ=255)
                                                 cGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAAcc                                        <  1:6552865/93‑1 (MQ=255)
                                                   gCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACcgc                                      <  1:2540521/93‑1 (MQ=255)
                                                   gCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACcgc                                      >  2:6632303/1‑93 (MQ=255)
                                                      aCCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGcc                                   >  1:10031662/1‑93 (MQ=255)
                                                      aCCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGcc                                   >  1:6676250/1‑93 (MQ=255)
                                                       ccccACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCg                                  <  1:3838443/93‑1 (MQ=255)
                                                         ccACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGGTATACACTGGCTTAACGTTGGTTTCCCCATTGAGATAAACCGCGCCGtg                                >  1:8579532/1‑92 (MQ=255)
                                                         ccACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTc                                <  2:552644/93‑1 (MQ=255)
                                                          cacaAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCg                               >  2:3668920/1‑93 (MQ=255)
                                                          cacaAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCg                               >  1:5121275/1‑93 (MQ=255)
                                                           acaAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGa                              <  2:6198162/93‑1 (MQ=255)
                                                            caAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGAt                             <  2:1028869/93‑1 (MQ=255)
                                                             aaaTCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGAtt                            <  2:9635461/93‑1 (MQ=255)
                                                                tCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTcc                         <  2:338282/93‑1 (MQ=255)
                                                                 cGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCg                        <  1:4364567/93‑1 (MQ=255)
                                                                 cGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCg                        <  2:7970486/93‑1 (MQ=255)
                                                                 cGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCg                        >  1:8625624/1‑93 (MQ=255)
                                                                  gTACTCATCGGCGTGCTCGACTTTTACACGCCAGATATCACCCGGCTTAACGTTGGATTCACCATTGAGATAAAACGCGCCGTCGATTTCCgg                       >  2:56518/1‑93 (MQ=255)
                                                                   tACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGt                      <  2:2788486/93‑1 (MQ=255)
                                                                    aCTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTg                     >  1:5646193/1‑93 (MQ=255)
                                                                     cTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGc                    <  1:3136240/93‑1 (MQ=255)
                                                                      tCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCa                   <  1:4590814/93‑1 (MQ=255)
                                                                        aTCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCAta                 <  1:6498399/93‑2 (MQ=255)
                                                                        aTCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATc                 <  2:1652399/93‑1 (MQ=255)
                                                                        aTCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATc                 <  2:5801062/93‑1 (MQ=255)
                                                                        aTCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATc                 <  1:9611845/93‑1 (MQ=255)
                                                                        aTCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATc                 >  2:3091404/1‑93 (MQ=255)
                                                                        aTCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATc                 <  1:2693678/93‑1 (MQ=255)
                                                                         tCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCt                >  2:7019637/1‑93 (MQ=255)
                                                                            gCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGcc             >  1:1333376/1‑93 (MQ=255)
                                                                             cGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCa            <  1:2618721/93‑1 (MQ=255)
                                                                             cGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCa            <  2:9154125/93‑1 (MQ=255)
                                                                             cGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCa            <  1:9520663/93‑1 (MQ=255)
                                                                               tGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtg          >  2:6245896/1‑93 (MQ=255)
                                                                               tGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtg          >  2:8162728/1‑93 (MQ=255)
                                                                                gCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgc         >  1:3336120/1‑93 (MQ=255)
                                                                                gCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgc         >  2:7707104/1‑93 (MQ=255)
                                                                                gCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgc         >  2:1197792/1‑93 (MQ=255)
                                                                                gCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgc         >  1:1776196/1‑93 (MQ=255)
                                                                                gCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgc         >  2:4239266/1‑93 (MQ=255)
                                                                                    gACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCg     <  2:2508972/93‑1 (MQ=255)
                                                                                     aCTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGa    >  2:4113095/1‑93 (MQ=255)
                                                                                     aCTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGa    <  2:8721899/93‑1 (MQ=255)
                                                                                     aCTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGa    <  2:2419363/93‑1 (MQ=255)
                                                                                          |                                                                                         
TATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTCACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACC  >  NC_000913/875891‑876070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTCACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCAATC  >  NC_000913/875890‑876074
                                                                                           |                                                                                             
TTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCTGCGTGCTCGAGTTTTAC                                                                                             >  SRR4302540.1897932/1‑94 (MQ=60)
TTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTAC                                                                                             >  SRR4302540.2633258/1‑94 (MQ=60)
TTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTAC                                                                                             <  SRR4302540.9673309/94‑1 (MQ=60)
TTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTAC                                                                                             >  SRR4302540.133124/1‑94 (MQ=60)
 TATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACA                                                                                            >  SRR4302540.5093921/1‑94 (MQ=60)
  ATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACAC                                                                                           <  SRR4302540.1171661/94‑1 (MQ=60)
  ATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACAC                                                                                           <  SRR4302540.8251568/94‑1 (MQ=60)
   atgcgaggccgcacaaatgagctaGCCCGGCGTGAATTAATTATACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACG                                                                                          <  SRR4302540.7554201/70‑1 (MQ=60)
   TTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACG                                                                                          <  SRR4302540.1932298/94‑1 (MQ=60)
   TTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTCTACACG                                                                                          <  SRR4302540.8376911/94‑1 (MQ=60)
     CGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGTGTGCTCGACTTTTACACGCA                                                                                        <  SRR4302540.9853370/94‑1 (MQ=60)
     CGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCA                                                                                        <  SRR4302540.4197265/94‑1 (MQ=60)
      GCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAG                                                                                       <  SRR4302540.6707515/94‑1 (MQ=60)
      GCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAG                                                                                       >  SRR4302540.1227036/1‑94 (MQ=60)
       CAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGG                                                                                      >  SRR4302540.9445764/1‑94 (MQ=60)
       CAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGG                                                                                      <  SRR4302540.2908062/94‑1 (MQ=60)
        AGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGA                                                                                     >  SRR4302540.888539/1‑94 (MQ=60)
        AGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTCACACGCAGGA                                                                                     >  SRR4302540.4826756/1‑94 (MQ=60)
         GGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGAT                                                                                    <  SRR4302540.6249039/94‑1 (MQ=60)
         GGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGAT                                                                                    <  SRR4302540.3870010/94‑1 (MQ=60)
          GCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATA                                                                                   <  SRR4302540.6102797/94‑1 (MQ=60)
          GCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATA                                                                                   <  SRR4302540.5781791/94‑1 (MQ=60)
           CTGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATAT                                                                                  <  SRR4302540.4176284/94‑1 (MQ=60)
             GGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCA                                                                                >  SRR4302540.8800989/1‑94 (MQ=60)
             GGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCA                                                                                <  SRR4302540.7194017/94‑1 (MQ=60)
             GGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCA                                                                                >  SRR4302540.5737790/1‑94 (MQ=60)
                 AAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCG                                                                            >  SRR4302540.9027125/1‑94 (MQ=60)
                 AAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCG                                                                            >  SRR4302540.4113065/1‑94 (MQ=60)
                  AATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGG                                                                           <  SRR4302540.4716020/94‑1 (MQ=60)
                  AATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGG                                                                           <  SRR4302540.1617305/94‑1 (MQ=60)
                   ATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGC                                                                          >  SRR4302540.3199292/1‑94 (MQ=60)
                     GATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTT                                                                        >  SRR4302540.6313012/1‑94 (MQ=60)
                      ATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTA                                                                       >  SRR4302540.2097902/1‑94 (MQ=60)
                      ATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTA                                                                       <  SRR4302540.6671532/94‑1 (MQ=60)
                      ATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACGTTTACACGCAAGATCTCATCCGGCTTA                                                                       >  SRR4302540.9806144/1‑94 (MQ=60)
                       TTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAA                                                                      <  SRR4302540.8263947/94‑1 (MQ=60)
                         TTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACG                                                                    <  SRR4302540.5333886/94‑1 (MQ=60)
                           GCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTT                                                                  <  SRR4302540.10014843/94‑1 (MQ=60)
                           GCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTT                                                                  <  SRR4302540.6391234/94‑1 (MQ=60)
                            CCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTG                                                                 <  SRR4302540.8607402/94‑1 (MQ=60)
                            CCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTG                                                                 <  SRR4302540.2834239/94‑1 (MQ=60)
                             CCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGG                                                                >  SRR4302540.3671962/1‑94 (MQ=60)
                              CGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGT                                                               >  SRR4302540.6592799/1‑94 (MQ=60)
                              CGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGT                                                               >  SRR4302540.3662589/1‑94 (MQ=60)
                               GGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTT                                                              >  SRR4302540.2230376/1‑94 (MQ=60)
                                GCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTT                                                             >  SRR4302540.7840009/1‑94 (MQ=60)
                                GCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTT                                                             <  SRR4302540.9358101/94‑1 (MQ=60)
                                GCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTT                                                             >  SRR4302540.6519679/1‑94 (MQ=60)
                                 CCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTC                                                            <  SRR4302540.8536907/94‑1 (MQ=60)
                                  CTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCA                                                           >  SRR4302540.6461753/1‑94 (MQ=60)
                                  CTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCA                                                           >  SRR4302540.8916629/1‑94 (MQ=60)
                                  CTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCAACCGGCTTAACGTTGGTTTCA                                                           >  SRR4302540.4147803/1‑94 (MQ=60)
                                  CTGAATTAATTAAACCCGGCTACCCCACAAATCATACTCATCGGCGTGCTCGAATTTTACACGCAGGGTATCACCCGGCTTAACGCTGGTTTCA                                                           >  SRR4302540.8708725/1‑94 (MQ=60)
                                      ATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCAT                                                       <  SRR4302540.8965110/94‑1 (MQ=60)
                                      ATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCAT                                                       <  SRR4302540.10108393/94‑1 (MQ=60)
                                       TTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATT                                                      <  SRR4302540.6772755/94‑1 (MQ=60)
                                        TAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTCACGTTGGTTTCACCATTG                                                     >  SRR4302540.4735697/1‑94 (MQ=60)
                                        TAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTG                                                     >  SRR4302540.3712515/1‑94 (MQ=60)
                                         AATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGA                                                    <  SRR4302540.5644335/94‑1 (MQ=60)
                                          ATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAG                                                   <  SRR4302540.2293268/94‑1 (MQ=60)
                                            TAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGAT                                                 <  SRR4302540.1128024/94‑1 (MQ=60)
                                             AAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATA                                                <  SRR4302540.1621333/94‑1 (MQ=60)
                                             AAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATA                                                >  SRR4302540.4205557/1‑94 (MQ=60)
                                              AACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAA                                               <  SRR4302540.10098456/94‑1 (MQ=60)
                                              AACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAA                                               <  SRR4302540.3562462/94‑1 (MQ=60)
                                              AACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAA                                               <  SRR4302540.3018959/94‑1 (MQ=60)
                                               ACCCGGCTACCCCACAAATCGTACTCATCGGCGTTCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAA                                              <  SRR4302540.8891766/94‑1 (MQ=60)
                                                CCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAAC                                             <  SRR4302540.4275695/94‑1 (MQ=60)
                                                CCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAAC                                             <  SRR4302540.1508774/94‑1 (MQ=60)
                                                CCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAAC                                             <  SRR4302540.2243775/94‑1 (MQ=60)
                                                CCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTGTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAAC                                             <  SRR4302540.3759947/94‑1 (MQ=60)
                                                 CCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACC                                            >  SRR4302540.6081431/1‑94 (MQ=60)
                                                  CGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCG                                           <  SRR4302540.6625539/94‑1 (MQ=60)
                                                   GGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATCAACCGC                                          >  SRR4302540.334449/1‑94 (MQ=60)
                                                    GCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCG                                         <  SRR4302540.2567917/94‑1 (MQ=60)
                                                    GCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGGTGGTTTCACCATTGAGATAAACCGCG                                         >  SRR4302540.2370204/1‑94 (MQ=60)
                                                    GCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTCACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCG                                         <  SRR4302540.3906808/94‑1 (MQ=60)
                                                     CTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGC                                        <  SRR4302540.2708935/94‑1 (MQ=60)
                                                      TACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCC                                       >  SRR4302540.10148356/1‑94 (MQ=60)
                                                      TACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCC                                       >  SRR4302540.6750001/1‑94 (MQ=60)
                                                      TACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCC                                       >  SRR4302540.6198790/1‑94 (MQ=60)
                                                       ACCCCACAAATCGTACTCATCGGCGTGCTCGGCTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCG                                      <  SRR4302540.2205984/94‑1 (MQ=60)
                                                       ACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCG                                      >  SRR4302540.8499147/1‑94 (MQ=60)
                                                       ACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTCGGTTCACCATTGAGATAAACCGCGCCG                                      >  SRR4302540.364780/1‑94 (MQ=60)
                                                        CCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGT                                     <  SRR4302540.3880190/94‑1 (MQ=60)
                                                         CCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGGTATACACTGGCTTAACGTTGGTTTCCCCATTGAGATAAACCGCGCCGTG                                    >  SRR4302540.8679261/1‑94 (MQ=60)
                                                          CCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCATCATTGAGATAAACCGAGCCGTCG                                   >  SRR4302540.919782/1‑94 (MQ=60)
                                                          CCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCG                                   >  SRR4302540.5176723/1‑94 (MQ=60)
                                                           CACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGA                                  <  SRR4302540.9541782/94‑1 (MQ=60)
                                                                 TCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCG                            >  SRR4302540.8725823/1‑94 (MQ=60)
                                                                 TCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCG                            <  SRR4302540.7572952/94‑1 (MQ=60)
                                                                  CGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGG                           >  SRR4302540.2245079/1‑94 (MQ=60)
                                                                  CGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGG                           <  SRR4302540.4597822/94‑1 (MQ=60)
                                                                  CGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGG                           <  SRR4302540.4412007/94‑1 (MQ=60)
                                                                   GTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGT                          >  SRR4302540.9700540/1‑94 (MQ=60)
                                                                    TACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTG                         >  SRR4302540.5708333/1‑94 (MQ=60)
                                                                    TACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTG                         <  SRR4302540.9657399/94‑1 (MQ=60)
                                                                      CTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCA                       >  SRR4302540.4680530/1‑94 (MQ=60)
                                                                      CTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCA                       <  SRR4302540.3170448/94‑1 (MQ=60)
                                                                      CTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCA                       <  SRR4302540.8837316/94‑1 (MQ=60)
                                                                       TCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCAT                      >  SRR4302540.7082509/1‑94 (MQ=60)
                                                                       TCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCAT                      <  SRR4302540.4640230/94‑1 (MQ=60)
                                                                         ATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCT                    >  SRR4302540.1906809/1‑94 (MQ=60)
                                                                         ATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCT                    >  SRR4302540.10159966/1‑94 (MQ=60)
                                                                         ATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCT                    <  SRR4302540.9725177/94‑1 (MQ=60)
                                                                         ATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCT                    <  SRR4302540.3907396/94‑1 (MQ=60)
                                                                         ATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCT                    <  SRR4302540.2722761/94‑1 (MQ=60)
                                                                         ATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCT                    <  SRR4302540.6339670/94‑1 (MQ=60)
                                                                         ATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCT                    >  SRR4302540.1256143/1‑94 (MQ=60)
                                                                         ATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATAT                    <  SRR4302540.6570650/94‑1 (MQ=60)
                                                                          TCGTCGTGCTCGACTTTTACACGCAGGATATCTCCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTG                   <  SRR4302540.2378439/94‑1 (MQ=60)
                                                                           CGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGC                  <  SRR4302540.3032943/94‑1 (MQ=60)
                                                                            GGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCC                 >  SRR4302540.1347321/1‑94 (MQ=60)
                                                                            GGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCC                 >  SRR4302540.5931970/1‑94 (MQ=60)
                                                                              GGTGCTCGACTTGTACACGCAGGATATCACCCGGCTTAACGTTGGTTCCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAT               <  SRR4302540.1049658/94‑1 (MQ=60)
                                                                              CGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAT               <  SRR4302540.9633409/94‑1 (MQ=60)
                                                                              CGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAT               <  SRR4302540.3683953/94‑1 (MQ=60)
                                                                              CGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAT               <  SRR4302540.2647023/94‑1 (MQ=60)
                                                                              CGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAT               <  SRR4302540.2647026/94‑1 (MQ=60)
                                                                              CGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAT               <  SRR4302540.5747237/94‑1 (MQ=60)
                                                                              CGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAT               <  SRR4302540.1749678/94‑1 (MQ=60)
                                                                               GTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATG              >  SRR4302540.1596241/1‑94 (MQ=60)
                                                                               GTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATC              >  SRR4302540.6483797/1‑94 (MQ=60)
                                                                                TGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGC             >  SRR4302540.3372758/1‑94 (MQ=60)
                                                                                TGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGC             >  SRR4302540.1795480/1‑94 (MQ=60)
                                                                                TGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGC             >  SRR4302540.2754691/1‑94 (MQ=60)
                                                                                 GCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCT            <  SRR4302540.8705779/94‑1 (MQ=60)
                                                                                  CTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTG           <  SRR4302540.4543989/94‑1 (MQ=60)
                                                                                    CGACTTTTACACGCAGGATATCACCCGTCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCG         >  SRR4302540.9234512/1‑94 (MQ=60)
                                                                                    CGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCG         <  SRR4302540.1147712/94‑1 (MQ=60)
                                                                                     GACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGC        >  SRR4302540.8447330/1‑94 (MQ=60)
                                                                                     GACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGA        >  SRR4302540.8967254/1‑94 (MQ=60)
                                                                                       CTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACC      >  SRR4302540.4217645/1‑94 (MQ=60)
                                                                                       CTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACC      <  SRR4302540.4401748/94‑1 (MQ=60)
                                                                                       CTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACC      >  SRR4302540.8572166/1‑94 (MQ=60)
                                                                                       CTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACC      <  SRR4302540.8254074/94‑1 (MQ=60)
                                                                                        TTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCA     >  SRR4302540.7543147/1‑94 (MQ=60)
                                                                                        TTTTACACGCAGGATATCACCCGGCTTAACGTTGGGTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGAGACCA     >  SRR4302540.2007343/1‑94 (MQ=60)
                                                                                          TTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCAAT   >  SRR4302540.6465116/1‑94 (MQ=60)
                                                                                          TTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCAAT   <  SRR4302540.1566683/94‑1 (MQ=60)
                                                                                           TACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCAATC  <  SRR4302540.4885675/94‑1 (MQ=60)
                                                                                           TACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCAATC  <  SRR4302540.7036528/94‑1 (MQ=60)
                                                                                           |                                                                                             
TTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTCACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCAATC  >  NC_000913/875890‑876074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑