Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 875,981 C→T V426V (GTG→GTA)  yliG ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913875,9810CT100.0% 722.7 / NA 192V426V (GTG→GTA) yliGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (106/86);  total (106/86)

Only 100 of 203 total aligned reads displayed.

TATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTCACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACC  >  NC_000913/875891‑876070
                                                                                          |                                                                                         
tattCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTtac                                                                                         >  1:5603552/1‑93 (MQ=255)
tattCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTtac                                                                                         <  2:1685988/93‑1 (MQ=255)
 attCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTtaca                                                                                        <  1:1190331/93‑1 (MQ=255)
 attCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTtaca                                                                                        <  1:4318952/93‑1 (MQ=255)
 attCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTtaca                                                                                        <  1:2884227/93‑1 (MQ=255)
  ttCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTacac                                                                                       >  2:6203371/1‑93 (MQ=255)
   tCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACg                                                                                      >  2:3434654/1‑93 (MQ=255)
     gCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCa                                                                                    <  1:4174820/93‑1 (MQ=255)
       aGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAgg                                                                                  >  1:166154/1‑93 (MQ=255)
        ggCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGa                                                                                 >  1:2103053/1‑93 (MQ=255)
        ggCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGa                                                                                 <  2:3020990/93‑1 (MQ=255)
          ccGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGata                                                                               <  2:5268058/93‑1 (MQ=255)
           cGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGatat                                                                              >  1:4498164/1‑93 (MQ=255)
            ggACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGatata                                                                             >  1:5311180/1‑92 (MQ=255)
            ggACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATc                                                                             <  1:4760697/93‑1 (MQ=255)
            ggACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATc                                                                             >  2:5664998/1‑93 (MQ=255)
                aaaTGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCAccc                                                                         >  2:3255566/1‑93 (MQ=255)
                aaaTGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCAccc                                                                         >  2:2427538/1‑93 (MQ=255)
                aaaTGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCAccc                                                                         <  1:5396523/93‑1 (MQ=255)
                 aaTGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCg                                                                        <  2:5180515/93‑1 (MQ=255)
                   tGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGc                                                                      <  2:5016354/93‑1 (MQ=255)
                   tGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGc                                                                      >  1:1322285/1‑93 (MQ=255)
                    gATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCt                                                                     <  2:2535690/93‑1 (MQ=255)
                    gATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCt                                                                     >  1:1907672/1‑93 (MQ=255)
                        ttGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAAc                                                                 <  2:4747589/93‑1 (MQ=255)
                        ttGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAAc                                                                 <  2:991057/93‑1 (MQ=255)
                          gCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGt                                                               <  2:1997690/93‑1 (MQ=255)
                           cccGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGtt                                                              <  2:793641/93‑1 (MQ=255)
                           cccGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGtt                                                              <  2:5875503/93‑1 (MQ=255)
                            ccGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTg                                                             <  2:1928435/93‑1 (MQ=255)
                             cGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTgg                                                            >  2:1046206/1‑93 (MQ=255)
                              ggCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGt                                                           <  1:4017967/93‑1 (MQ=255)
                              ggCCTGAATTAATTAAACCCGGCGACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGt                                                           >  2:5097372/1‑93 (MQ=255)
                                 cTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTc                                                        >  1:4427672/1‑93 (MQ=255)
                                  tGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCATCCGGCTTAACGTTGGTTTCa                                                       >  2:2557717/1‑93 (MQ=255)
                                  tGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCa                                                       >  1:1209636/1‑93 (MQ=255)
                                    aattaattAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCAcc                                                     >  2:1749402/1‑93 (MQ=255)
                                    aattaattAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCAcc                                                     <  2:1136492/93‑1 (MQ=255)
                                     attaattaAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCa                                                    >  1:3470945/1‑93 (MQ=255)
                                      ttaattaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCAt                                                   >  1:4696818/1‑93 (MQ=255)
                                        aattaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTg                                                 <  2:573753/93‑1 (MQ=255)
                                        aattaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTg                                                 >  1:5947997/1‑93 (MQ=255)
                                        aattaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTg                                                 >  2:1351501/1‑93 (MQ=255)
                                         attaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTga                                                <  2:3350196/93‑1 (MQ=255)
                                         attaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTga                                                <  2:3809469/93‑1 (MQ=255)
                                         attaaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTga                                                <  2:101543/93‑1 (MQ=255)
                                           taaACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTgaga                                              <  1:3181476/93‑1 (MQ=255)
                                                ccGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAAc                                         <  2:161885/93‑1 (MQ=255)
                                                 cGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAAcc                                        >  2:492003/1‑93 (MQ=255)
                                                 cGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAAcc                                        >  1:4626821/1‑93 (MQ=255)
                                                  ggCTACNCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACcg                                       <  1:739422/93‑1 (MQ=255)
                                                  ggCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACcg                                       <  2:3120486/93‑1 (MQ=255)
                                                  ggCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAAAGTTGGTTTCACCATTGAGATAAAAcg                                       >  2:1971449/1‑93 (MQ=255)
                                                   gCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATGACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACcgc                                      >  1:3250007/1‑93 (MQ=255)
                                                   gCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTACACCATTGAGATAAACcgc                                      <  2:6109749/93‑1 (MQ=255)
                                                   gCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGGTTCACCATTGAGATAAACcgc                                      >  2:5397690/1‑93 (MQ=255)
                                                     tACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCgcgc                                    >  2:5553980/1‑93 (MQ=255)
                                                     tACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCgcgc                                    >  2:4103484/1‑93 (MQ=255)
                                                      aCCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGcc                                   >  2:1866161/1‑93 (MQ=255)
                                                      aCCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGcc                                   >  1:5439441/1‑93 (MQ=255)
                                                      aCCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGcc                                   >  2:2362549/1‑93 (MQ=255)
                                                      aCCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGcc                                   >  2:4868642/1‑93 (MQ=255)
                                                       ccccACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCg                                  >  2:2601596/1‑93 (MQ=255)
                                                        cccACAAATCGTACTCATCGGCGTGCTCGACTTTTACTCGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGt                                 <  2:5514886/93‑1 (MQ=255)
                                                        cccACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGt                                 <  1:2209019/93‑1 (MQ=255)
                                                         ccACAAATCGTACTCATCGGCGTGCTCGTCTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTc                                <  2:2014027/93‑1 (MQ=255)
                                                         ccACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTc                                >  1:2534875/1‑93 (MQ=255)
                                                         ccACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTc                                <  1:831590/93‑1 (MQ=255)
                                                           acaAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGa                              >  2:1400416/1‑93 (MQ=255)
                                                           acaAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGa                              <  1:6206549/93‑1 (MQ=255)
                                                           acaAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGa                              >  2:3923108/1‑93 (MQ=255)
                                                            caAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGAt                             >  2:464639/1‑93 (MQ=255)
                                                              aaTCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGAttt                           <  1:2722856/93‑1 (MQ=255)
                                                               aTCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTc                          <  2:1572659/93‑1 (MQ=255)
                                                                tCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTcc                         <  1:4540607/93‑1 (MQ=255)
                                                                tCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTcc                         >  1:686938/1‑93 (MQ=255)
                                                                   tACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGt                      <  2:3823559/93‑1 (MQ=255)
                                                                   tACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGt                      >  2:4208536/1‑93 (MQ=255)
                                                                   tACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGt                      >  1:578626/1‑93 (MQ=255)
                                                                   tACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGt                      >  1:598498/1‑93 (MQ=255)
                                                                   tACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGt                      <  2:1222490/93‑1 (MQ=255)
                                                                     cTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGc                    <  2:5568446/93‑1 (MQ=255)
                                                                      tCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCa                   >  2:3575231/1‑93 (MQ=255)
                                                                       cATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCAt                  <  1:2422396/93‑1 (MQ=255)
                                                                        aTCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATc                 <  1:1002140/93‑1 (MQ=255)
                                                                        aTCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATc                 >  2:2148935/1‑93 (MQ=255)
                                                                          cGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGCGATAAACCGCGCCGTCGATTTCCGGTGCATCTg               >  1:349470/1‑93 (MQ=255)
                                                                          cGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTg               >  1:4939087/1‑93 (MQ=255)
                                                                           ggCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGc              >  1:1494065/1‑93 (MQ=255)
                                                                           ggCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGc              <  1:4795171/93‑1 (MQ=255)
                                                                             cGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCa            <  2:5077914/93‑1 (MQ=255)
                                                                               tGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtg          >  1:1557961/1‑93 (MQ=255)
                                                                                gCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgc         <  2:215171/93‑1 (MQ=255)
                                                                                gCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgc         <  2:3450883/93‑1 (MQ=255)
                                                                                  tCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgctg       >  1:28460/1‑93 (MQ=255)
                                                                                  tCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgctg       >  2:646898/1‑93 (MQ=255)
                                                                                   cGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgctgc      <  1:1694448/93‑1 (MQ=255)
                                                                                   cGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCAtgctgc      <  1:5145711/93‑1 (MQ=255)
                                                                                    gACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCg     <  1:2373140/93‑1 (MQ=255)
                                                                                     aCTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGa    >  1:3789361/1‑93 (MQ=255)
                                                                                          |                                                                                         
TATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTCACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACC  >  NC_000913/875891‑876070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTCACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCAATC  >  NC_000913/875888‑876074
                                                                                             |                                                                                             
CATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTT                                                                                               >  SRR4302533.5108604/1‑94 (MQ=60)
 ATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTA                                                                                              >  SRR4302533.5308934/1‑94 (MQ=60)
  TTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTAC                                                                                             <  SRR4302533.5048635/94‑1 (MQ=60)
  TTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTAC                                                                                             >  SRR4302533.5637408/1‑94 (MQ=60)
    ATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACAC                                                                                           <  SRR4302533.2901963/94‑1 (MQ=60)
    ATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACAC                                                                                           <  SRR4302533.4344965/94‑1 (MQ=60)
    ATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACAC                                                                                           <  SRR4302533.1197484/94‑1 (MQ=60)
    AGTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACAC                                                                                           <  SRR4302533.3851828/94‑1 (MQ=60)
     TTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACG                                                                                          <  SRR4302533.1341106/94‑1 (MQ=60)
        GCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAG                                                                                       <  SRR4302533.4200117/94‑1 (MQ=60)
        GCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAG                                                                                       >  SRR4302533.4703715/1‑94 (MQ=60)
         CAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGG                                                                                      >  SRR4302533.167207/1‑94 (MQ=60)
          AGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGA                                                                                     >  SRR4302533.2116020/1‑94 (MQ=60)
             CCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATAT                                                                                  >  SRR4302533.4525405/1‑94 (MQ=60)
              CGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATC                                                                                 <  SRR4302533.5097248/94‑1 (MQ=60)
              CGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATC                                                                                 >  SRR4302533.2403020/1‑94 (MQ=60)
              CGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATA                                                                                 >  SRR4302533.5343008/1‑94 (MQ=60)
               GGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCA                                                                                <  SRR4302533.4558683/94‑1 (MQ=60)
               GGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCA                                                                                <  SRR4302533.4789579/94‑1 (MQ=60)
               GGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCA                                                                                <  SRR4302533.712212/94‑1 (MQ=60)
                   AAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCG                                                                            <  SRR4302533.5428951/94‑1 (MQ=60)
                   AAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCG                                                                            <  SRR4302533.1205402/94‑1 (MQ=60)
                     ATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGC                                                                          >  SRR4302533.1330219/1‑94 (MQ=60)
                      TGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCT                                                                         >  SRR4302533.1919310/1‑94 (MQ=60)
                        ATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTA                                                                       >  SRR4302533.4854328/1‑94 (MQ=60)
                          TTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAAC                                                                     <  SRR4302533.1161755/94‑1 (MQ=60)
                            TGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGT                                                                   <  SRR4302533.4796949/94‑1 (MQ=60)
                                CGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGT                                                               <  SRR4302533.4596552/94‑1 (MQ=60)
                                 GGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTT                                                              <  SRR4302533.4042429/94‑1 (MQ=60)
                                 GGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTT                                                              >  SRR4302533.3497806/1‑94 (MQ=60)
                                  GCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTT                                                             >  SRR4302533.5472456/1‑94 (MQ=60)
                                   CCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTC                                                            <  SRR4302533.4740965/94‑1 (MQ=60)
                                   CCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTC                                                            >  SRR4302533.4387913/1‑94 (MQ=60)
                                   CCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTC                                                            >  SRR4302533.4454435/1‑94 (MQ=60)
                                    CTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCA                                                           >  SRR4302533.1216899/1‑94 (MQ=60)
                                     TGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCAC                                                          <  SRR4302533.5292981/94‑1 (MQ=60)
                                      GAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACC                                                         >  SRR4302533.1520429/1‑94 (MQ=60)
                                       AATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCA                                                        >  SRR4302533.2784290/1‑94 (MQ=60)
                                       AATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCA                                                        >  SRR4302533.3492370/1‑94 (MQ=60)
                                        ATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCAT                                                       >  SRR4302533.2502391/1‑94 (MQ=60)
                                        ATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCAT                                                       >  SRR4302533.4725292/1‑94 (MQ=60)
                                         TTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATT                                                      >  SRR4302533.5460563/1‑94 (MQ=60)
                                         TTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATT                                                      >  SRR4302533.2021555/1‑94 (MQ=60)
                                          TAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTG                                                     >  SRR4302533.5983975/1‑94 (MQ=60)
                                           AATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGA                                                    >  SRR4302533.2185231/1‑94 (MQ=60)
                                           AATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGA                                                    >  SRR4302533.4123220/1‑94 (MQ=60)
                                              TAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGAT                                                 <  SRR4302533.3201048/94‑1 (MQ=60)
                                               AAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATA                                                >  SRR4302533.901602/1‑94 (MQ=60)
                                                AACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAA                                               <  SRR4302533.3689540/94‑1 (MQ=60)
                                                AACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAA                                               <  SRR4302533.5404252/94‑1 (MQ=60)
                                                  CCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAAC                                             <  SRR4302533.2251625/94‑1 (MQ=60)
                                                   CCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACC                                            >  SRR4302533.4654862/1‑94 (MQ=60)
                                                     GGCTACNCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGC                                          <  SRR4302533.743775/94‑1 (MQ=60)
                                                     GGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATGACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGC                                          >  SRR4302533.3270047/1‑94 (MQ=60)
                                                        TACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCC                                       >  SRR4302533.5472199/1‑94 (MQ=60)
                                                          CCCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGT                                     >  SRR4302533.676488/1‑94 (MQ=60)
                                                           CCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTC                                    >  SRR4302533.4245106/1‑94 (MQ=60)
                                                           CCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTC                                    <  SRR4302533.2222643/94‑1 (MQ=60)
                                                           CCCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTC                                    >  SRR4302533.2550480/1‑94 (MQ=60)
                                                            CCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGGTAAACCGCGCCGTCG                                   >  SRR4302533.3295027/1‑94 (MQ=60)
                                                            CCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCG                                   >  SRR4302533.817171/1‑94 (MQ=60)
                                                            CCACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCG                                   <  SRR4302533.836465/94‑1 (MQ=60)
                                                              ACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGAT                                 <  SRR4302533.6243769/94‑1 (MQ=60)
                                                              ACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGAT                                 >  SRR4302533.5769865/1‑94 (MQ=60)
                                                              ACAAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGAT                                 <  SRR4302533.1290798/94‑1 (MQ=60)
                                                                AAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTT                               >  SRR4302533.6000440/1‑94 (MQ=60)
                                                                AAATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTT                               <  SRR4302533.1760053/94‑1 (MQ=60)
                                                                 AATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTC                              <  SRR4302533.2739565/94‑1 (MQ=60)
                                                                 AATCGTACTCATCGGCGTGCTCCACTTTTACACGCAGGATATCACCCGGCTTACCCCTGGTTTCCCCAATGAGATAAACCGCGGCGTCGATTTC                              >  SRR4302533.2224258/1‑94 (MQ=60)
                                                                  ATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCC                             >  SRR4302533.691000/1‑94 (MQ=60)
                                                                  ATCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCC                             <  SRR4302533.2880578/94‑1 (MQ=60)
                                                                   TCGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCG                            <  SRR4302533.4568099/94‑1 (MQ=60)
                                                                    CGTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGG                           >  SRR4302533.1602932/1‑94 (MQ=60)
                                                                     GTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGT                          >  SRR4302533.602023/1‑94 (MQ=60)
                                                                     GTACTCATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGT                          >  SRR4302533.582037/1‑94 (MQ=60)
                                                                          CATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATC                     <  SRR4302533.2437349/94‑1 (MQ=60)
                                                                           CTCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCT                    <  SRR4302533.323641/94‑1 (MQ=60)
                                                                           CTCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCT                    <  SRR4302533.3085254/94‑1 (MQ=60)
                                                                           ATCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCT                    <  SRR4302533.1007991/94‑1 (MQ=60)
                                                                            TCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGCGATAAACCGCGCCGTCGATTTCCGGTGCATCTG                   >  SRR4302533.351602/1‑94 (MQ=60)
                                                                            TCGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTG                   >  SRR4302533.4968987/1‑94 (MQ=60)
                                                                             CGGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGC                  >  SRR4302533.1503071/1‑94 (MQ=60)
                                                                              GGCGTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCC                 <  SRR4302533.4824219/94‑1 (MQ=60)
                                                                                 GTGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATG              >  SRR4302533.1567341/1‑94 (MQ=60)
                                                                                 GTGCTCGACTTTTACACGCAGGATACCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCCTG              <  SRR4302533.6126169/94‑1 (MQ=60)
                                                                                  TGCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGC             <  SRR4302533.5936570/94‑1 (MQ=60)
                                                                                   GCTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCT            >  SRR4302533.529339/1‑94 (MQ=60)
                                                                                    CTCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTG           >  SRR4302533.28652/1‑94 (MQ=60)
                                                                                     TCGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGC          >  SRR4302533.4480029/1‑94 (MQ=60)
                                                                                      CGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGCTTTCCGGTGCATCTGCCATGCTGCG         >  SRR4302533.2606772/1‑94 (MQ=60)
                                                                                      CGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCG         <  SRR4302533.5176810/94‑1 (MQ=60)
                                                                                      CGACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCG         <  SRR4302533.1704713/94‑1 (MQ=60)
                                                                                       GACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGA        >  SRR4302533.3812563/1‑94 (MQ=60)
                                                                                       GACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGA        <  SRR4302533.2387826/94‑1 (MQ=60)
                                                                                        ACTTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGAC       >  SRR4302533.1701898/1‑94 (MQ=60)
                                                                                          TTTTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGCGCATCTGCCACGCTGCGACCA     >  SRR4302533.5175345/1‑94 (MQ=60)
                                                                                            TTACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCTCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCAAT   <  SRR4302533.4578275/94‑1 (MQ=60)
                                                                                             TACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCAATC  <  SRR4302533.2457555/94‑1 (MQ=60)
                                                                                             |                                                                                             
CATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTAAACCCGGCTACCCCACAAATCGTACTCATCGGCGTGCTCGACTTTCACACGCAGGATATCACCCGGCTTAACGTTGGTTTCACCATTGAGATAAACCGCGCCGTCGATTTCCGGTGCATCTGCCATGCTGCGACCAATC  >  NC_000913/875888‑876074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑