Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 927,456 T→C K321K (AAA→AAG)  cydC ← ATP‑binding component of cytochrome‑related transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913927,4560TC100.0% 697.9 / NA 217K321K (AAA→AAG) cydCATP‑binding component of cytochrome‑related transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (113/104);  total (113/104)

Only 100 of 231 total aligned reads displayed.

TTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTC  >  NC_000913/927366‑927545
                                                                                          |                                                                                         
ttGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGctt                                                                                         >  2:7101252/1‑93 (MQ=255)
 tGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGcttt                                                                                        <  2:4438669/93‑1 (MQ=255)
 tGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGcttt                                                                                        >  1:8929964/1‑93 (MQ=255)
  gCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGctttt                                                                                       <  2:4199616/93‑1 (MQ=255)
  gCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGctttt                                                                                       <  2:8513134/93‑1 (MQ=255)
   cTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGGGAAACGCGATCGGCAACACGAGTTTGGGGATCAGGAAAGGTGACCTCCGCCTTTTg                                                                                      >  1:1309749/1‑93 (MQ=255)
   cTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTg                                                                                      <  1:6789913/93‑1 (MQ=255)
    tCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGa                                                                                     <  2:4592161/93‑1 (MQ=255)
    tCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGa                                                                                     >  2:2638817/1‑93 (MQ=255)
    tCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGa                                                                                     >  2:6331127/1‑93 (MQ=255)
        gATAAGTGAACTGACCATCCCGTAACGGCAGAGAAACGCGCTGGGCAACACGAGCTTGTGTATTGGGTAAGACTACCGCCGGATTTTTTTCCg                                                                                 >  2:233866/1‑93 (MQ=255)
        gATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCg                                                                                 >  1:4423045/1‑93 (MQ=255)
        gATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCg                                                                                 >  2:2189599/1‑93 (MQ=255)
          tAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGtt                                                                               >  2:1870662/1‑93 (MQ=255)
          tAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGtt                                                                               >  1:3326003/1‑93 (MQ=255)
            aGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTaa                                                                             >  2:5257792/1‑93 (MQ=255)
                  ctgcacATCCCCTAGCTTCAACGAACCGCGATCGGCAACACGAGGTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCaga                                                                       <  1:314730/93‑1 (MQ=255)
                   tgaacATCCCGTAACGTCAGCGAAAGGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCagag                                                                      <  2:2207255/93‑1 (MQ=255)
                    gaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAgaga                                                                     >  2:9390046/1‑93 (MQ=255)
                    gaacAACCCGTAACTTCAGCGAACCGCGATCGTCAACACGAGTTTGGGTATCAGGAAAGGTCACCTCCGGCTTTTGATCCGTTAAGTCAgaga                                                                     <  2:7484406/93‑1 (MQ=255)
                      acATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGAAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAta                                                                   >  1:4401448/1‑93 (MQ=255)
                         tCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgt                                                                >  2:3653122/1‑93 (MQ=255)
                          cccGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgta                                                               <  2:7969251/93‑1 (MQ=255)
                          cccGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgta                                                               <  1:3120408/93‑1 (MQ=255)
                          cccGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgta                                                               >  1:4661372/1‑93 (MQ=255)
                          cccGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgta                                                               <  2:8177273/93‑1 (MQ=255)
                          cccGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgta                                                               <  2:2727304/93‑1 (MQ=255)
                            cGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgtacg                                                             >  2:2681910/1‑93 (MQ=255)
                             gTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACgg                                                            <  2:1203455/93‑1 (MQ=255)
                             gTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACgg                                                            >  1:9233836/1‑93 (MQ=255)
                              tAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGc                                                           >  2:7113317/1‑93 (MQ=255)
                              tAACGTCAGCGAAACGCGATCGGCAACACGAGTTAGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGc                                                           >  2:6373284/1‑93 (MQ=255)
                               aaCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCa                                                          <  1:4955832/93‑1 (MQ=255)
                                aCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGTAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCag                                                         >  2:1764567/1‑93 (MQ=255)
                                aCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCag                                                         >  1:661063/1‑93 (MQ=255)
                                 cGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCaga                                                        <  1:7130404/93‑1 (MQ=255)
                                 cGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCaga                                                        <  1:6031702/93‑1 (MQ=255)
                                 cGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCaga                                                        >  2:6885317/1‑93 (MQ=255)
                                   tCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAgg                                                      >  1:3682344/1‑93 (MQ=255)
                                   tCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAgg                                                      >  1:1465254/1‑93 (MQ=255)
                                     aGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCa                                                    <  2:9144633/93‑1 (MQ=255)
                                      gCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCaa                                                   <  1:8028051/93‑1 (MQ=255)
                                      gCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCaa                                                   <  2:6492561/93‑1 (MQ=255)
                                       cGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAAt                                                  <  1:7759957/93‑1 (MQ=255)
                                        gAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATg                                                 >  2:8697737/1‑93 (MQ=255)
                                        gAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATg                                                 >  1:1771939/1‑93 (MQ=255)
                                        gAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATg                                                 >  1:3919584/1‑93 (MQ=255)
                                         aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGa                                                <  2:3099014/93‑1 (MQ=255)
                                          aaCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGAc                                               <  2:9278633/93‑1 (MQ=255)
                                           aCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACt                                              <  1:6428462/93‑1 (MQ=255)
                                           aCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACt                                              >  1:543098/1‑93 (MQ=255)
                                           aCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACt                                              <  1:2585565/93‑1 (MQ=255)
                                           aCGCGATCGGCAACACGAGTTTGCGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACt                                              <  2:5508435/93‑1 (MQ=255)
                                            cgcgATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACtt                                             <  2:1945507/93‑1 (MQ=255)
                                               gATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGcc                                          >  1:9412601/1‑93 (MQ=255)
                                                aTCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGcgc                                         >  2:3440110/1‑91 (MQ=255)
                                                   ggCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAg                                      >  2:4908277/1‑93 (MQ=255)
                                                    gCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGa                                     <  2:2886569/93‑1 (MQ=255)
                                                    gCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGa                                     >  1:2664750/1‑93 (MQ=255)
                                                    gCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGa                                     <  1:9570876/93‑1 (MQ=255)
                                                      aaCACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATg                                   >  1:9974411/1‑93 (MQ=255)
                                                      aaCACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGACAGAGGCAATGACTTGCCCCAGATg                                   >  2:9416433/1‑93 (MQ=255)
                                                       acacGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGc                                  <  1:4278781/93‑1 (MQ=255)
                                                       acacGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGc                                  >  1:7338482/1‑93 (MQ=255)
                                                         acGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTg                                >  2:5728023/1‑93 (MQ=255)
                                                          cGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGa                               >  1:9839077/1‑93 (MQ=255)
                                                           gAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGaa                              <  1:8444206/93‑1 (MQ=255)
                                                           gAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTG‑CCCAGATGCTGaaa                             >  2:7016611/1‑93 (MQ=255)
                                                            aGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGaaa                             <  2:5900731/93‑1 (MQ=255)
                                                            aGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGaaa                             <  1:1098373/93‑1 (MQ=255)
                                                             gTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAAt                            <  2:3697479/93‑1 (MQ=255)
                                                                tGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCa                         <  2:8589655/93‑1 (MQ=255)
                                                                  gtgaTCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGGCAGAGATACGTACGGCAGAGGCAATGCCTTGCCCCAGATGCTGAAATGCAcc                       <  2:7852959/90‑1 (MQ=255)
                                                                  ggTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCAcc                       <  2:5422217/93‑1 (MQ=255)
                                                                    tATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCg                     >  1:1887122/1‑93 (MQ=255)
                                                                      tCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGtt                   <  1:8653688/93‑1 (MQ=255)
                                                                       cAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTa                  >  1:9665792/1‑93 (MQ=255)
                                                                       cAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTa                  <  1:8193998/93‑1 (MQ=255)
                                                                        aGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGATTTGCCCCAGATGCTGAAATGCACCCGTTAc                 >  2:6136345/1‑93 (MQ=255)
                                                                         ggAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACt                >  2:9904547/1‑93 (MQ=255)
                                                                         ggAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACt                <  1:2136556/93‑1 (MQ=255)
                                                                         ggAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACt                >  1:2263317/1‑93 (MQ=255)
                                                                          gAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTg               >  2:8958822/1‑93 (MQ=255)
                                                                           aaaGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTgg              <  1:5645672/93‑1 (MQ=255)
                                                                           aaaGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTgg              <  1:2321649/93‑1 (MQ=255)
                                                                            aaGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTggg             >  1:2743746/1‑92 (MQ=255)
                                                                             aGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTg            >  1:5260679/1‑93 (MQ=255)
                                                                             aGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTg            >  1:5127658/1‑93 (MQ=255)
                                                                             aGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTg            >  1:6179859/1‑93 (MQ=255)
                                                                             aGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTg            >  2:5522458/1‑93 (MQ=255)
                                                                              ggTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGc           >  1:7045862/1‑93 (MQ=255)
                                                                              ggTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGc           >  1:1974772/1‑93 (MQ=255)
                                                                              ggTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGc           <  2:2498518/93‑1 (MQ=255)
                                                                               gTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGcc          >  2:7042043/1‑93 (MQ=255)
                                                                               gTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGcc          >  2:6987085/1‑93 (MQ=255)
                                                                                  aCCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGt       >  1:5887750/1‑93 (MQ=255)
                                                                                   ccTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCCGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTg      >  2:2452177/1‑93 (MQ=255)
                                                                                       cGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTc  <  1:322078/93‑1 (MQ=255)
                                                                                       cGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTc  >  1:10024342/1‑93 (MQ=255)
                                                                                       cGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTc  >  2:3232192/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTC  >  NC_000913/927366‑927545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAA  >  NC_000913/927363‑927548
                                                                                             |                                                                                            
AGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGC                                                                                              >  SRR4302540.139043/1‑94 (MQ=60)
AGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGC                                                                                              >  SRR4302540.2090726/1‑94 (MQ=60)
 GATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCT                                                                                             >  SRR4302540.3746115/1‑94 (MQ=60)
  ATTGCTCCGGATAAGTGAACTGAACAGCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTT                                                                                            <  SRR4302540.2300214/94‑1 (MQ=60)
   TTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTT                                                                                           >  SRR4302540.9034105/1‑94 (MQ=60)
   TTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACACGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTT                                                                                           >  SRR4302540.4371110/1‑94 (MQ=60)
     GCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGGGAAACGCGATCGGCAACACGAGTTTGGGGATCAGGAAAGGTGACCTCCGCCTTTTG                                                                                         >  SRR4302540.1323353/1‑94 (MQ=60)
      CTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGA                                                                                        >  SRR4302540.4748780/1‑94 (MQ=60)
      CTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGA                                                                                        <  SRR4302540.5954229/94‑1 (MQ=60)
      CTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGA                                                                                        <  SRR4302540.6865002/94‑1 (MQ=60)
       TCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGAT                                                                                       >  SRR4302540.231036/1‑94 (MQ=60)
        CCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATC                                                                                      <  SRR4302540.5075131/94‑1 (MQ=60)
        CCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGGTTGGGTATCAGGAAAGGGGACCTCCGGTTTTTGATC                                                                                      >  SRR4302540.5449297/1‑94 (MQ=60)
          GGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCG                                                                                    >  SRR4302540.8263119/1‑94 (MQ=60)
          GGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCG                                                                                    >  SRR4302540.4470970/1‑94 (MQ=60)
           GATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGT                                                                                   >  SRR4302540.3953639/1‑94 (MQ=60)
            ATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTT                                                                                  <  SRR4302540.7096665/94‑1 (MQ=60)
            ATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTT                                                                                  >  SRR4302540.3362501/1‑94 (MQ=60)
            ATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTT                                                                                  <  SRR4302540.5003917/94‑1 (MQ=60)
             TAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTA                                                                                 <  SRR4302540.2751840/94‑1 (MQ=60)
                    ACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGA                                                                          >  SRR4302540.124568/1‑94 (MQ=60)
                     CTGCACATCCCCTAGCTTCAACGAACCGCGATCGGCAACACGAGGTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAG                                                                         <  SRR4302540.317864/94‑1 (MQ=60)
                       GAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAT                                                                       <  SRR4302540.618165/94‑1 (MQ=60)
                        AACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGAAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATA                                                                      >  SRR4302540.4449179/1‑94 (MQ=60)
                         ACATCCCGTAACGTCAGCGAAACGCGATCGGCAACAGGAGTTTGGGTATCAGGAAAGGTGACCTCCTGCTTTTGATCCGTTAAGTAAGAGATAC                                                                     >  SRR4302540.1855091/1‑94 (MQ=60)
                          CATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACG                                                                    >  SRR4302540.7280428/1‑94 (MQ=60)
                          CATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACG                                                                    >  SRR4302540.788052/1‑94 (MQ=60)
                           ATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGT                                                                   <  SRR4302540.3650071/94‑1 (MQ=60)
                           ATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGT                                                                   >  SRR4302540.2982640/1‑94 (MQ=60)
                           ATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGCTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGT                                                                   >  SRR4302540.9726685/1‑94 (MQ=60)
                            TCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTA                                                                  >  SRR4302540.4711272/1‑94 (MQ=60)
                             CCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTAC                                                                 <  SRR4302540.3154512/94‑1 (MQ=60)
                              CCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGCCCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACG                                                                <  SRR4302540.1080465/94‑1 (MQ=60)
                               CGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGG                                                               >  SRR4302540.2506887/1‑94 (MQ=60)
                               CGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGG                                                               >  SRR4302540.9344490/1‑94 (MQ=60)
                                  AACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAG                                                            >  SRR4302540.8244343/1‑94 (MQ=60)
                                  AACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAG                                                            <  SRR4302540.5009387/94‑1 (MQ=60)
                                  AACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAG                                                            >  SRR4302540.668139/1‑94 (MQ=60)
                                   ACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGA                                                           <  SRR4302540.4564042/94‑1 (MQ=60)
                                    CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                          >  SRR4302540.217784/1‑94 (MQ=60)
                                    CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                          <  SRR4302540.7209833/94‑1 (MQ=60)
                                    CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                          <  SRR4302540.8402191/94‑1 (MQ=60)
                                    CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                          <  SRR4302540.6098646/94‑1 (MQ=60)
                                    CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                          <  SRR4302540.6098644/94‑1 (MQ=60)
                                    CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                          >  SRR4302540.263448/1‑94 (MQ=60)
                                    AGTCAGCGAAACGCGATCGCCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTTCGGCAGAG                                                          <  SRR4302540.7209832/94‑1 (MQ=60)
                                     GTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGG                                                         >  SRR4302540.5375964/1‑94 (MQ=60)
                                     GTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGG                                                         >  SRR4302540.3722586/1‑94 (MQ=60)
                                     GTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGG                                                         >  SRR4302540.2985312/1‑94 (MQ=60)
                                     GTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGG                                                         >  SRR4302540.1481239/1‑94 (MQ=60)
                                      TCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGC                                                        <  SRR4302540.8990646/94‑1 (MQ=60)
                                       CAGCGAAACGCGATCGGCAACACGAGTTTGGGTGTCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCA                                                       <  SRR4302540.9783459/94‑1 (MQ=60)
                                       CAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCA                                                       <  SRR4302540.4270592/94‑1 (MQ=60)
                                       CAGCGAAACGCGATCGGCAACACGAGTTTGGGAAGCAGGAAAGGTGACCTCGGGCTTTGGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCA                                                       >  SRR4302540.3055320/1‑94 (MQ=60)
                                         GCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAAT                                                     <  SRR4302540.6529280/94‑1 (MQ=60)
                                         GCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAAT                                                     <  SRR4302540.8121406/94‑1 (MQ=60)
                                          CGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATG                                                    >  SRR4302540.3962012/1‑94 (MQ=60)
                                          CGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATG                                                    <  SRR4302540.7850636/94‑1 (MQ=60)
                                          CGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATG                                                    >  SRR4302540.1791169/1‑94 (MQ=60)
                                           GAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGA                                                   >  SRR4302540.937213/1‑94 (MQ=60)
                                           GAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGA                                                   >  SRR4302540.1625859/1‑94 (MQ=60)
                                            tttcctGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGAC                                                  <  SRR4302540.3549217/88‑1 (MQ=60)
                                            AAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGAC                                                  <  SRR4302540.3180590/94‑1 (MQ=60)
                                             AACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACT                                                 >  SRR4302540.548999/1‑94 (MQ=60)
                                              ACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTT                                                >  SRR4302540.3613154/1‑94 (MQ=60)
                                              ACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTT                                                <  SRR4302540.2613435/94‑1 (MQ=60)
                                              ACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTT                                                <  SRR4302540.6500011/94‑1 (MQ=60)
                                               CGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTG                                               >  SRR4302540.3190457/1‑94 (MQ=60)
                                                GCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGC                                              >  SRR4302540.6739432/1‑94 (MQ=60)
                                                 CGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCC                                             >  SRR4302540.9524513/1‑94 (MQ=60)
                                                    TCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCA                                          >  SRR4302540.77989/1‑94 (MQ=60)
                                                      GGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGTAATGACTTGCCCCAGA                                        >  SRR4302540.9031852/1‑94 (MQ=60)
                                                      GGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGA                                        >  SRR4302540.5590252/1‑94 (MQ=60)
                                                      GGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGA                                        >  SRR4302540.2693644/1‑94 (MQ=60)
                                                       tcggcattcctgctgaaccgctcttccgatctagcgctTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGAT                                       <  SRR4302540.8639480/56‑1 (MQ=60)
                                                       GCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGAT                                       <  SRR4302540.9205260/94‑1 (MQ=60)
                                                       GCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGAT                                       <  SRR4302540.9683946/94‑1 (MQ=60)
                                                       GCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGAT                                       <  SRR4302540.9697668/94‑1 (MQ=60)
                                                       GCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGAT                                       <  SRR4302540.7304519/94‑1 (MQ=60)
                                                        GAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATG                                      <  SRR4302540.5938567/94‑1 (MQ=60)
                                                        CAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATG                                      >  SRR4302540.10090746/1‑94 (MQ=60)
                                                        CAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATG                                      <  SRR4302540.9538753/94‑1 (MQ=60)
                                                         AACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGC                                     >  SRR4302540.7420378/1‑94 (MQ=60)
                                                          acagaagttgggttaccaggaaggttgccccCCGGTTTTTGATCC‑TTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTG                                   <  SRR4302540.7704966/63‑1 (MQ=60)
                                                          ACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCT                                    <  SRR4302540.4325318/94‑1 (MQ=60)
                                                            ACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGA                                  >  SRR4302540.1991712/1‑94 (MQ=60)
                                                            ACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGA                                  >  SRR4302540.9954119/1‑94 (MQ=60)
                                                             CGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAA                                 <  SRR4302540.2536789/94‑1 (MQ=60)
                                                              GAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAA                                <  SRR4302540.8542231/94‑1 (MQ=60)
                                                               AGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAAT                               <  SRR4302540.3356603/94‑1 (MQ=60)
                                                               AGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAAT                               <  SRR4302540.1109682/94‑1 (MQ=60)
                                                                GTTTGGGCATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGCTACGTACAGCAGAGGCAATGACTTGACGCCGATGGTGAAATG                              >  SRR4302540.1480615/1‑94 (MQ=60)
                                                                 TTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGC                             >  SRR4302540.6845842/1‑94 (MQ=60)
                                                                   TGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCAC                           >  SRR4302540.8717174/1‑94 (MQ=60)
                                                                   TGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCAC                           >  SRR4302540.5266715/1‑94 (MQ=60)
                                                                    GGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACC                          >  SRR4302540.4399929/1‑94 (MQ=60)
                                                                      GTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCG                        >  SRR4302540.1907783/1‑94 (MQ=60)
                                                                         TCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTA                     >  SRR4302540.9779551/1‑94 (MQ=60)
                                                                         TCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTA                     <  SRR4302540.8754213/94‑1 (MQ=60)
                                                                          CAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAC                    <  SRR4302540.4469660/94‑1 (MQ=60)
                                                                          CAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAC                    <  SRR4302540.8289210/94‑1 (MQ=60)
                                                                           AGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACT                   >  SRR4302540.2287608/1‑94 (MQ=60)
                                                                            GGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTG                  <  SRR4302540.2159502/94‑1 (MQ=60)
                                                                            GGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTG                  >  SRR4302540.2037052/1‑94 (MQ=60)
                                                                             GAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGG                 <  SRR4302540.631468/94‑1 (MQ=60)
                                                                              AAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGT                <  SRR4302540.9963244/94‑1 (MQ=60)
                                                                              AAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGT                <  SRR4302540.5707808/94‑1 (MQ=60)
                                                                              AAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGT                <  SRR4302540.2346652/94‑1 (MQ=60)
                                                                              AAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGG                >  SRR4302540.2773377/1‑94 (MQ=60)
                                                                               AAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTG               >  SRR4302540.6248697/1‑94 (MQ=60)
                                                                               AAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTG               >  SRR4302540.5318075/1‑94 (MQ=60)
                                                                               AAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTG               >  SRR4302540.5183192/1‑94 (MQ=60)
                                                                                AGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGC              >  SRR4302540.7124211/1‑94 (MQ=60)
                                                                                AGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGC              >  SRR4302540.1631058/1‑94 (MQ=60)
                                                                                AGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGC              >  SRR4302540.1996202/1‑94 (MQ=60)
                                                                                   TGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAG           <  SRR4302540.2100036/94‑1 (MQ=60)
                                                                                    GACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGT          >  SRR4302540.5952800/1‑94 (MQ=60)
                                                                                         CCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTC     >  SRR4302540.10140990/1‑94 (MQ=60)
                                                                                          CGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCA    <  SRR4302540.325297/94‑1 (MQ=60)
                                                                                          CGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCA    >  SRR4302540.5924265/1‑94 (MQ=60)
                                                                                          CGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCA    >  SRR4302540.8572146/1‑94 (MQ=60)
                                                                                           GGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAA   >  SRR4302540.9897813/1‑94 (MQ=60)
                                                                                            GCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAA  <  SRR4302540.2261065/94‑1 (MQ=60)
                                                                                             |                                                                                            
AGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAA  >  NC_000913/927363‑927548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑