Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 927,456 T→C K321K (AAA→AAG)  cydC ← ATP‑binding component of cytochrome‑related transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913927,4560TC100.0% 703.1 / NA 216K321K (AAA→AAG) cydCATP‑binding component of cytochrome‑related transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (108/108);  total (108/108)

Only 100 of 224 total aligned reads displayed.

TTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTC  >  NC_000913/927366‑927545
                                                                                          |                                                                                         
 tGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGcttt                                                                                        <  1:2567704/93‑1 (MQ=255)
   cTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTg                                                                                      >  2:1533804/1‑93 (MQ=255)
   cTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTg                                                                                      >  2:6042408/1‑93 (MQ=255)
   cTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTg                                                                                      >  2:399289/1‑93 (MQ=255)
   cTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTg                                                                                      <  1:5071385/93‑1 (MQ=255)
    tCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGa                                                                                     <  2:5095038/93‑1 (MQ=255)
       ggATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATcc                                                                                  >  1:319247/1‑93 (MQ=255)
        gATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCg                                                                                 >  1:2684278/1‑93 (MQ=255)
           aaGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTa                                                                              <  1:3527708/93‑1 (MQ=255)
             atgaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAg                                                                            <  1:5632955/92‑1 (MQ=255)
              tgaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCATCACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGt                                                                           <  2:2903871/93‑1 (MQ=255)
                aactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCa                                                                         >  2:5329191/1‑93 (MQ=255)
                aactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCa                                                                         <  1:2380894/93‑1 (MQ=255)
                 actgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCag                                                                        >  1:4867119/1‑93 (MQ=255)
                  ctgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCaga                                                                       <  1:89396/93‑1 (MQ=255)
                  ctgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCaga                                                                       <  2:1122224/93‑1 (MQ=255)
                  cggaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCaga                                                                       <  1:326500/91‑1 (MQ=255)
                   tgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACATGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCagag                                                                      >  1:6157889/1‑93 (MQ=255)
                    gaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAgaga                                                                     <  2:2516786/93‑1 (MQ=255)
                     aacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAt                                                                    >  2:2621178/1‑93 (MQ=255)
                     aacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAt                                                                    >  1:993348/1‑93 (MQ=255)
                      acATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAta                                                                   <  1:4337448/93‑1 (MQ=255)
                        aTCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacg                                                                 >  1:1053026/1‑93 (MQ=255)
                        aTCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacg                                                                 <  2:859577/93‑1 (MQ=255)
                         tCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgt                                                                >  2:607782/1‑93 (MQ=255)
                         tCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgt                                                                >  1:804958/1‑93 (MQ=255)
                         tCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGATATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgt                                                                >  1:4446845/1‑93 (MQ=255)
                          cccGTAATGTCAGCGAAGCGCGATCGGCATCACGAGTTTGGGTATCAGGAAATGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgta                                                               <  1:5959529/93‑1 (MQ=255)
                          cccGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgta                                                               <  1:6014671/93‑1 (MQ=255)
                           ccGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAtacgtac                                                              >  2:5000855/1‑93 (MQ=255)
                              tAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGc                                                           >  1:3031638/1‑93 (MQ=255)
                              tAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGc                                                           <  2:156298/93‑1 (MQ=255)
                               aaCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCa                                                          >  2:2020346/1‑93 (MQ=255)
                               aaCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCa                                                          <  2:4564873/93‑1 (MQ=255)
                                 cggcAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCaga                                                        <  1:1846179/90‑1 (MQ=255)
                                 cGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCaga                                                        <  1:3675607/93‑1 (MQ=255)
                                 cGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCaga                                                        <  1:798123/93‑1 (MQ=255)
                                 cGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCaga                                                        <  1:2431353/93‑1 (MQ=255)
                                 cGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCaga                                                        <  1:3421948/93‑1 (MQ=255)
                                  gTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCagag                                                       >  1:2647607/1‑93 (MQ=255)
                                   tCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAgg                                                      >  2:3180239/1‑93 (MQ=255)
                                    cAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGc                                                     <  1:5171444/93‑1 (MQ=255)
                                      gCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCaa                                                   >  1:451178/1‑93 (MQ=255)
                                      gCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCaa                                                   <  2:2147594/93‑1 (MQ=255)
                                      gCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCaa                                                   <  2:167473/93‑1 (MQ=255)
                                       cGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAAt                                                  <  1:3867715/93‑1 (MQ=255)
                                         aaaCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGa                                                <  2:4817197/93‑1 (MQ=255)
                                          aaCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGAc                                               <  1:2788/93‑1 (MQ=255)
                                           aCGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACt                                              <  2:524191/93‑1 (MQ=255)
                                            cgcgATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACtt                                             <  2:5395827/93‑1 (MQ=255)
                                            cgcgATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACtt                                             <  2:6301065/93‑1 (MQ=255)
                                             gcgATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTg                                            >  1:1115549/1‑93 (MQ=255)
                                                 tCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGcccc                                        >  2:6018683/1‑93 (MQ=255)
                                                 tCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGcccc                                        <  1:1528662/93‑1 (MQ=255)
                                                  cGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCa                                       <  2:67314/93‑1 (MQ=255)
                                                   ggCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAg                                      <  2:1880253/93‑1 (MQ=255)
                                                    gCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGa                                     >  1:1820692/1‑93 (MQ=255)
                                                    gCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGa                                     >  2:4492886/1‑93 (MQ=255)
                                                    gCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGa                                     <  2:2316365/93‑1 (MQ=255)
                                                    gCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGa                                     <  1:1197851/93‑1 (MQ=255)
                                                     cAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGAt                                    >  1:5155046/1‑93 (MQ=255)
                                                      aaCACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATg                                   >  2:3075243/1‑93 (MQ=255)
                                                       acacGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGc                                  <  1:5493946/93‑1 (MQ=255)
                                                        cacGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCt                                 >  1:1105332/1‑93 (MQ=255)
                                                            aGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGaaa                             <  2:806206/93‑1 (MQ=255)
                                                            aGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGaaa                             <  1:2205312/93‑1 (MQ=255)
                                                            aGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGaaa                             <  1:474870/93‑1 (MQ=255)
                                                            aGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGaaa                             >  1:5090280/1‑93 (MQ=255)
                                                              tttGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATg                           <  2:4422197/93‑1 (MQ=255)
                                                                tGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCa                         <  2:4579799/93‑1 (MQ=255)
                                                                   gTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCAccc                      <  1:2239931/93‑1 (MQ=255)
                                                                   gTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCAccc                      <  2:361268/93‑1 (MQ=255)
                                                                     aTCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGt                    >  1:3934057/1‑93 (MQ=255)
                                                                     aTCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGt                    <  2:4734812/93‑1 (MQ=255)
                                                                      tCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGtt                   <  1:4426190/93‑1 (MQ=255)
                                                                      tCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGtt                   >  2:3215184/1‑93 (MQ=255)
                                                                        aGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAc                 >  2:3841573/1‑93 (MQ=255)
                                                                        aGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAc                 >  1:4201178/1‑93 (MQ=255)
                                                                        aGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAc                 >  1:3247337/1‑93 (MQ=255)
                                                                        aGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAc                 >  1:709946/1‑93 (MQ=255)
                                                                        aGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAc                 >  2:1388694/1‑93 (MQ=255)
                                                                         ggAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACt                >  2:1722452/1‑93 (MQ=255)
                                                                           aaaGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTgg              <  1:2051836/93‑1 (MQ=255)
                                                                            aaGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGt             <  1:2675206/93‑1 (MQ=255)
                                                                             aGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTg            >  2:2762633/1‑93 (MQ=255)
                                                                             aGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTg            <  2:2258194/93‑1 (MQ=255)
                                                                              ggTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGc           <  1:3741867/93‑1 (MQ=255)
                                                                               gTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGcc          <  2:5167013/93‑1 (MQ=255)
                                                                                 gACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAg        >  1:3087996/1‑93 (MQ=255)
                                                                                  aCCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGt       <  1:4635075/93‑1 (MQ=255)
                                                                                  aCCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGt       >  1:5620206/1‑93 (MQ=255)
                                                                                   ccTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTg      >  1:6181520/1‑93 (MQ=255)
                                                                                    cTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGc     >  2:373209/1‑93 (MQ=255)
                                                                                    cTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGc     <  1:1979368/93‑1 (MQ=255)
                                                                                     tCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCt    <  2:1822191/93‑1 (MQ=255)
                                                                                     tCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCt    <  2:3361751/93‑1 (MQ=255)
                                                                                     tCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACACGTTACTGGTGCCAGTGCt    >  2:3895429/1‑93 (MQ=255)
                                                                                      ccGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCtt   >  1:4102064/1‑93 (MQ=255)
                                                                                       cGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTc  >  2:5733005/1‑93 (MQ=255)
                                                                                       cGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTc  <  1:5578625/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTC  >  NC_000913/927366‑927545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAAC  >  NC_000913/927364‑927549
                                                                                            |                                                                                             
GATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCT                                                                                              >  SRR4302533.1083922/1‑94 (MQ=60)
GATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCT                                                                                              <  SRR4302533.5490838/94‑1 (MQ=60)
 ATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTT                                                                                             >  SRR4302533.3148558/1‑94 (MQ=60)
   TGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTT                                                                                           <  SRR4302533.2583506/94‑1 (MQ=60)
   TGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTT                                                                                           <  SRR4302533.5396456/94‑1 (MQ=60)
   TGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTT                                                                                           >  SRR4302533.878444/1‑94 (MQ=60)
     CTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGA                                                                                         <  SRR4302533.5102039/94‑1 (MQ=60)
       CCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATC                                                                                       >  SRR4302533.20228/1‑94 (MQ=60)
        CGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCC                                                                                      >  SRR4302533.321203/1‑94 (MQ=60)
         GGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCG                                                                                     >  SRR4302533.2700728/1‑94 (MQ=60)
            TAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTA                                                                                  >  SRR4302533.2340476/1‑94 (MQ=60)
            TAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTA                                                                                  >  SRR4302533.2924710/1‑94 (MQ=60)
            TAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTA                                                                                  <  SRR4302533.6236392/94‑1 (MQ=60)
             AAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAA                                                                                 <  SRR4302533.3549452/94‑1 (MQ=60)
              AGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAG                                                                                <  SRR4302533.4871285/94‑1 (MQ=60)
               ATGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGT                                                                               <  SRR4302533.5667018/94‑1 (MQ=60)
                TGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTC                                                                              <  SRR4302533.6326949/94‑1 (MQ=60)
                 GAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCA                                                                             >  SRR4302533.6196306/1‑94 (MQ=60)
                 GAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCA                                                                             <  SRR4302533.3757409/94‑1 (MQ=60)
                  AACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAG                                                                            >  SRR4302533.4896611/1‑94 (MQ=60)
                  AACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAG                                                                            <  SRR4302533.2395622/94‑1 (MQ=60)
                   ACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGA                                                                           >  SRR4302533.3358550/1‑94 (MQ=60)
                    CTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACATGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAG                                                                          >  SRR4302533.6194929/1‑94 (MQ=60)
                    CTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAG                                                                          <  SRR4302533.89962/94‑1 (MQ=60)
                    CGGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAG                                                                          <  SRR4302533.328486/94‑1 (MQ=60)
                     TGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGA                                                                         >  SRR4302533.1123212/1‑94 (MQ=60)
                      GAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAT                                                                        >  SRR4302533.999153/1‑94 (MQ=60)
                      GAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGAT                                                                        <  SRR4302533.5084773/94‑1 (MQ=60)
                       AACATCCCGTAACGTCAGCGAAACGCGATCGGCAACGCTAGTGTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATA                                                                       <  SRR4302533.1835689/94‑1 (MQ=60)
                        ACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATAC                                                                      <  SRR4302533.4363590/94‑1 (MQ=60)
                        ACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATAC                                                                      <  SRR4302533.4060563/94‑1 (MQ=60)
                         CATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACG                                                                     >  SRR4302533.1059229/1‑94 (MQ=60)
                          ATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGT                                                                    >  SRR4302533.809687/1‑94 (MQ=60)
                          ATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGATATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGT                                                                    >  SRR4302533.4473740/1‑94 (MQ=60)
                            CCCGTAATGTCAGCGAAGCGCGATCGGCATCACGAGTTTGGGTATCAGGAAATGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTAC                                                                  <  SRR4302533.5995578/94‑1 (MQ=60)
                            CCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTAC                                                                  <  SRR4302533.6051121/94‑1 (MQ=60)
                             CCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAACGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACG                                                                 <  SRR4302533.4468376/94‑1 (MQ=60)
                               GTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGC                                                               >  SRR4302533.3050188/1‑94 (MQ=60)
                                   CGTCAGCGAAACGCGATCGGCAGCACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                           <  SRR4302533.985560/94‑1 (MQ=60)
                                   CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                           <  SRR4302533.3443087/94‑1 (MQ=60)
                                   CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                           <  SRR4302533.802818/94‑1 (MQ=60)
                                   CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                           <  SRR4302533.3698195/94‑1 (MQ=60)
                                   CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                           >  SRR4302533.2663873/1‑94 (MQ=60)
                                   CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                           <  SRR4302533.2446381/94‑1 (MQ=60)
                                   CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                           >  SRR4302533.1631092/1‑94 (MQ=60)
                                   CGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                           >  SRR4302533.806155/1‑94 (MQ=60)
                                   CGGCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAG                                                           <  SRR4302533.1857437/94‑1 (MQ=60)
                                      CAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCA                                                        <  SRR4302533.5202651/94‑1 (MQ=60)
                                       AGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAA                                                       >  SRR4302533.3615602/1‑94 (MQ=60)
                                       AGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAA                                                       >  SRR4302533.453878/1‑94 (MQ=60)
                                       AGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAA                                                       >  SRR4302533.3810993/1‑94 (MQ=60)
                                        GCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAAT                                                      <  SRR4302533.461462/94‑1 (MQ=60)
                                         CGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATG                                                     <  SRR4302533.3891325/94‑1 (MQ=60)
                                           AAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGAC                                                   <  SRR4302533.5160574/94‑1 (MQ=60)
                                            AACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACT                                                  <  SRR4302533.2809/94‑1 (MQ=60)
                                            AACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACT                                                  <  SRR4302533.3448751/94‑1 (MQ=60)
                                              CGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTG                                                <  SRR4302533.72614/94‑1 (MQ=60)
                                              CGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTG                                                >  SRR4302533.1122168/1‑94 (MQ=60)
                                               GCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGC                                               >  SRR4302533.3239830/1‑94 (MQ=60)
                                               GCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGC                                               <  SRR4302533.2791575/94‑1 (MQ=60)
                                                 GATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCC                                             >  SRR4302533.569540/1‑94 (MQ=60)
                                                 GATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCC                                             >  SRR4302533.5657555/1‑94 (MQ=60)
                                                 GATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCC                                             <  SRR4302533.2437213/94‑1 (MQ=60)
                                                  ATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCC                                            >  SRR4302533.263052/1‑94 (MQ=60)
                                                   TCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCA                                           <  SRR4302533.1537858/94‑1 (MQ=60)
                                                   TCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCA                                           >  SRR4302533.1984283/1‑94 (MQ=60)
                                                    CGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAG                                          <  SRR4302533.2250365/94‑1 (MQ=60)
                                                     GGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGA                                         >  SRR4302533.1831814/1‑94 (MQ=60)
                                                      GCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGAT                                        <  SRR4302533.6188379/94‑1 (MQ=60)
                                                      GCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGAT                                        >  SRR4302533.5186176/1‑94 (MQ=60)
                                                      GCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGAT                                        <  SRR4302533.1205048/94‑1 (MQ=60)
                                                       CAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATG                                       <  SRR4302533.1003352/94‑1 (MQ=60)
                                                       CAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATG                                       >  SRR4302533.2123452/1‑94 (MQ=60)
                                                       CAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATG                                       >  SRR4302533.5298396/1‑94 (MQ=60)
                                                       CAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATG                                       >  SRR4302533.2577924/1‑94 (MQ=60)
                                                       CAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACATACGGCAGAGGCAATGACTTGCCCCAGATG                                       >  SRR4302533.3073465/1‑94 (MQ=60)
                                                         ACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCT                                     <  SRR4302533.5527087/94‑1 (MQ=60)
                                                         ACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCT                                     >  SRR4302533.1111885/1‑94 (MQ=60)
                                                            CGAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAA                                  <  SRR4302533.3219411/94‑1 (MQ=60)
                                                             GAGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAA                                 >  SRR4302533.5121036/1‑94 (MQ=60)
                                                              AGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAAT                                >  SRR4302533.6010790/1‑94 (MQ=60)
                                                              AGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAAT                                <  SRR4302533.2218911/94‑1 (MQ=60)
                                                              AGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAAT                                >  SRR4302533.5186483/1‑94 (MQ=60)
                                                              AGTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAAT                                <  SRR4302533.477706/94‑1 (MQ=60)
                                                               GTTTGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATG                               >  SRR4302533.4238426/1‑94 (MQ=60)
                                                                  TGGGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCAC                            >  SRR4302533.2682665/1‑94 (MQ=60)
                                                                    GGTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCC                          <  SRR4302533.1556464/94‑1 (MQ=60)
                                                                     GTATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCG                         <  SRR4302533.2253773/94‑1 (MQ=60)
                                                                      TATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGT                        >  SRR4302533.3958069/1‑94 (MQ=60)
                                                                       ATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTT                       <  SRR4302533.4706888/94‑1 (MQ=60)
                                                                       ATCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTT                       >  SRR4302533.1403473/1‑94 (MQ=60)
                                                                        TCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTA                      <  SRR4302533.4452943/94‑1 (MQ=60)
                                                                        TCAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTA                      >  SRR4302533.4152502/1‑94 (MQ=60)
                                                                         CAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAC                     >  SRR4302533.4226598/1‑94 (MQ=60)
                                                                         CAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAC                     >  SRR4302533.714138/1‑94 (MQ=60)
                                                                         CAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAC                     >  SRR4302533.1109315/1‑94 (MQ=60)
                                                                         CAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAC                     >  SRR4302533.3267364/1‑94 (MQ=60)
                                                                         CAGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTAC                     >  SRR4302533.1118582/1‑94 (MQ=60)
                                                                          AGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACT                    >  SRR4302533.4529780/1‑94 (MQ=60)
                                                                          AGGAAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACT                    <  SRR4302533.4496707/94‑1 (MQ=60)
                                                                             AAAGGTGCCCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGT                 <  SRR4302533.5606642/94‑1 (MQ=60)
                                                                             AAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGT                 <  SRR4302533.2705380/94‑1 (MQ=60)
                                                                             AAAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGT                 <  SRR4302533.2064498/94‑1 (MQ=60)
                                                                              AAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTG                >  SRR4302533.5628257/1‑94 (MQ=60)
                                                                              AAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTG                <  SRR4302533.2558851/94‑1 (MQ=60)
                                                                              AAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTG                <  SRR4302533.4152064/94‑1 (MQ=60)
                                                                              AAGGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTG                <  SRR4302533.2691616/94‑1 (MQ=60)
                                                                                GGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCTCCAGATGCTGAAATGCACCCGTTACTGGGGCC              >  SRR4302533.2693319/1‑94 (MQ=60)
                                                                                GGTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCC              <  SRR4302533.3764815/94‑1 (MQ=60)
                                                                                 GTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCA             <  SRR4302533.32740/94‑1 (MQ=60)
                                                                                 GTGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCA             <  SRR4302533.4381131/94‑1 (MQ=60)
                                                                                  TGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAG            >  SRR4302533.3106980/1‑94 (MQ=60)
                                                                                  TGACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAG            >  SRR4302533.3562616/1‑94 (MQ=60)
                                                                                   GACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGT           >  SRR4302533.5654175/1‑94 (MQ=60)
                                                                                    ACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTG          <  SRR4302533.4663161/94‑1 (MQ=60)
                                                                                    ACCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTG          >  SRR4302533.6218641/1‑94 (MQ=60)
                                                                                     CCTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGC         >  SRR4302533.5765061/1‑94 (MQ=60)
                                                                                      CTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCT        >  SRR4302533.2128433/1‑94 (MQ=60)
                                                                                      CTCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCT        <  SRR4302533.1991489/94‑1 (MQ=60)
                                                                                       TCCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTT       >  SRR4302533.4127024/1‑94 (MQ=60)
                                                                                        CCGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTC      >  SRR4302533.3945355/1‑94 (MQ=60)
                                                                                         CGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCA     <  SRR4302533.5070626/94‑1 (MQ=60)
                                                                                         CGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCA     <  SRR4302533.5612339/94‑1 (MQ=60)
                                                                                         CGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCA     >  SRR4302533.4965753/1‑94 (MQ=60)
                                                                                         CGGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGACGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCA     <  SRR4302533.5105305/94‑1 (MQ=60)
                                                                                          GGCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAA    <  SRR4302533.5372073/94‑1 (MQ=60)
                                                                                           GCTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAA   >  SRR4302533.1040390/1‑94 (MQ=60)
                                                                                            CTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAAC  >  SRR4302533.1673734/1‑94 (MQ=60)
                                                                                            CTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAAC  >  SRR4302533.4920568/1‑94 (MQ=60)
                                                                                            CTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAAC  <  SRR4302533.2540979/94‑1 (MQ=60)
                                                                                            CTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAAC  >  SRR4302533.2572427/1‑94 (MQ=60)
                                                                                            |                                                                                             
GATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAAC  >  NC_000913/927364‑927549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑