Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 988,555 A→G L342P (CTG→CCG)  pncB ← nicotinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913988,5550AG99.6% 880.5 / ‑4.1 275coding (1025/1203 nt)pncBnicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (139/135);  minor base . (0/1);  total (139/136)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 290 total aligned reads displayed.

CTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG  >  NC_000913/988465‑988644
                                                                                          |                                                                                         
cTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCggg                                                                                         <  1:5324874/93‑1 (MQ=255)
   tCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGa                                                                                      >  2:6661732/1‑93 (MQ=255)
   tCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGa                                                                                      <  1:2112229/93‑1 (MQ=255)
   tCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGa                                                                                      <  1:5913682/93‑1 (MQ=255)
    cAGAAAGTTTCGCCTCCGGTTGACCGTTACTCTCTACCAACTTAATGACAATTTTGAGGGTTGTTGCCTGGGGGATATCGCATGTCGGGCGAg                                                                                     <  2:5833460/93‑1 (MQ=255)
    cAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAg                                                                                     <  2:4063008/93‑1 (MQ=255)
      gAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTc                                                                                   <  2:2801597/93‑1 (MQ=255)
      gAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTc                                                                                   >  2:1792600/1‑93 (MQ=255)
        aaGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTccc                                                                                 >  2:7593225/1‑93 (MQ=255)
          gTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCaa                                                                               <  1:4383400/93‑1 (MQ=255)
           tttCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAAt                                                                              <  2:4433671/93‑1 (MQ=255)
            ttCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATa                                                                             >  1:9748080/1‑93 (MQ=255)
                 cACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaa                                                                        >  1:4977844/1‑93 (MQ=255)
                  cccGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       <  2:4147498/92‑1 (MQ=255)
                   ccGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAAc                                                                      >  2:6337541/1‑93 (MQ=255)
                   ccGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAAc                                                                      >  1:6319701/1‑93 (MQ=255)
                     ggTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGACCCAATACCAAAACtt                                                                    >  1:3329279/1‑93 (MQ=255)
                      gTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTa                                                                   >  1:8203906/1‑93 (MQ=255)
                      gTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTa                                                                   >  1:9843560/1‑93 (MQ=255)
                       tttACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTaa                                                                  <  2:7408171/93‑1 (MQ=255)
                        ttACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAAt                                                                 <  2:1208268/93‑1 (MQ=255)
                         tACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAAtt                                                                >  2:5259612/1‑93 (MQ=255)
                          aCCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTg                                                               <  1:9304721/93‑1 (MQ=255)
                          aCCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTg                                                               >  2:8649862/1‑93 (MQ=255)
                           ccGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGc                                                              <  1:4308394/93‑1 (MQ=255)
                           ccGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGc                                                              <  1:6513673/93‑1 (MQ=255)
                            cGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCa                                                             <  1:9641787/93‑1 (MQ=255)
                            cGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCa                                                             <  2:7656418/93‑1 (MQ=255)
                            cGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCa                                                             <  2:8463946/93‑1 (MQ=255)
                             gTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAc                                                            >  2:3587761/1‑93 (MQ=255)
                             gTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAc                                                            >  2:2673776/1‑93 (MQ=255)
                             gTTACACTATACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAc                                                            >  2:7329240/1‑93 (MQ=255)
                              ttACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcg                                                           <  1:5042515/93‑1 (MQ=255)
                              ttACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcg                                                           <  1:7621397/93‑1 (MQ=255)
                              ttACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcg                                                           <  1:7505350/93‑1 (MQ=255)
                              ttACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcg                                                           <  1:4612149/93‑1 (MQ=255)
                              ttACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcg                                                           >  1:1068733/1‑93 (MQ=255)
                               tACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcgc                                                          <  2:3121774/93‑1 (MQ=255)
                                acacTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcgcg                                                         >  1:2874300/1‑93 (MQ=255)
                                 cacTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCgg                                                        <  2:4545313/93‑1 (MQ=255)
                                  cctctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCggg                                                       <  2:7182572/92‑1 (MQ=255)
                                   ctctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGa                                                      >  2:1351387/1‑93 (MQ=255)
                                   ctctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGa                                                      >  1:8333433/1‑93 (MQ=255)
                                    tctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGaa                                                     <  2:7045371/93‑1 (MQ=255)
                                    tctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGaa                                                     >  1:2595727/1‑93 (MQ=255)
                                       aCCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAagag                                                  >  1:9402856/1‑93 (MQ=255)
                                         cAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGaa                                                >  1:7326120/1‑93 (MQ=255)
                                         cAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGaa                                                <  2:2033291/93‑1 (MQ=255)
                                          aaCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAg                                               <  2:8877604/93‑1 (MQ=255)
                                           aCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGACGt                                              >  2:8189272/1‑93 (MQ=255)
                                           aCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGt                                              <  1:9137215/93‑1 (MQ=255)
                                            cTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTg                                             <  1:5818278/93‑1 (MQ=255)
                                             ttAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTgg                                            >  1:2160827/1‑93 (MQ=255)
                                             ttAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTgg                                            >  1:8711772/1‑93 (MQ=255)
                                              tAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGc                                           <  1:6082124/93‑1 (MQ=255)
                                                aTGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGCAGTGGCgg                                         >  1:68797/1‑93 (MQ=255)
                                                  gACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGta                                       <  2:9903401/93‑1 (MQ=255)
                                                   aCAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGtat                                      <  1:3896003/93‑1 (MQ=255)
                                                   aCAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGtat                                      <  2:5516982/93‑1 (MQ=255)
                                                   aCAATATTCAGGGGTTTTACCTGGGCGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGtat                                      <  2:4486413/93‑1 (MQ=255)
                                                         ttCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCa                                >  2:7001183/1‑93 (MQ=255)
                                                          tCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCaa                               <  2:6565801/93‑1 (MQ=255)
                                                          tCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCaa                               >  1:9214102/1‑93 (MQ=255)
                                                           cAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAc                              <  2:9662835/93‑1 (MQ=255)
                                                           cAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAc                              <  1:4346181/93‑1 (MQ=255)
                                                            aGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAcc                             <  2:4687238/93‑1 (MQ=255)
                                                            aGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAcc                             >  1:4020883/1‑93 (MQ=255)
                                                             ggggTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTGAATTGCACGCGGGAAGAGAAGTGGCGGTAGAGCTCAACCg                            >  2:8921666/1‑93 (MQ=255)
                                                                gTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                         >  2:753467/1‑93 (MQ=255)
                                                                gTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                         <  1:8179322/93‑1 (MQ=255)
                                                                gTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                         >  2:4938472/1‑93 (MQ=255)
                                                                   ttACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGc                      >  1:8110699/1‑93 (MQ=255)
                                                                     aCCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGt                    >  2:1473059/1‑93 (MQ=255)
                                                                      ccTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTa                   <  2:8707332/93‑1 (MQ=255)
                                                                      ccTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTa                   <  1:1838438/93‑1 (MQ=255)
                                                                       cTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTaa                  <  2:4858021/93‑1 (MQ=255)
                                                                       cTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTaa                  >  1:5528451/1‑93 (MQ=255)
                                                                       cTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTaa                  >  2:3170981/1‑93 (MQ=255)
                                                                        tGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTaaa                 >  1:1797066/1‑93 (MQ=255)
                                                                        tGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTaaa                 >  1:10021509/1‑93 (MQ=255)
                                                                         gggggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAAt                >  1:7641118/1‑93 (MQ=255)
                                                                         gggggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAAt                <  2:6760781/93‑1 (MQ=255)
                                                                           gggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATcc              <  2:2509090/93‑1 (MQ=255)
                                                                           gggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATcc              >  1:1343027/1‑93 (MQ=255)
                                                                            ggATATCGCAGGGCGGGCGAGTCCCAATACCAAACCTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCa             <  1:2923191/93‑1 (MQ=255)
                                                                             gATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAg            >  1:7384220/1‑93 (MQ=255)
                                                                             gATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAg            >  2:8324041/1‑93 (MQ=255)
                                                                              atatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGa           >  2:426718/1‑93 (MQ=255)
                                                                               tatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGAt          <  1:9025643/93‑1 (MQ=255)
                                                                               tatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGAGGCGGTATAGCTCAACCGGTTTGCGTAAATGCCGAt          >  2:6905361/1‑93 (MQ=255)
                                                                                 tCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTg        >  1:2332646/1‑93 (MQ=255)
                                                                                     aGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCag    <  1:5713751/93‑1 (MQ=255)
                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   <  2:1522514/93‑1 (MQ=255)
                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   <  2:9318525/93‑1 (MQ=255)
                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   >  2:2374597/1‑93 (MQ=255)
                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   <  2:7952303/93‑1 (MQ=255)
                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   >  1:1811761/1‑93 (MQ=255)
                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   <  2:1899803/93‑1 (MQ=255)
                                                                                       gTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCagag  >  1:365270/1‑93 (MQ=255)
                                                                                       gTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCagag  >  2:3867108/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG  >  NC_000913/988465‑988644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  >  NC_000913/988462‑988648
                                                                                             |                                                                                             
GGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCG                                                                                               <  SRR4302540.6511310/94‑1 (MQ=60)
GGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCG                                                                                               <  SRR4302540.5281931/94‑1 (MQ=60)
GGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCG                                                                                               <  SRR4302540.5281932/94‑1 (MQ=60)
 GGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGG                                                                                              >  SRR4302540.4384859/1‑94 (MQ=60)
 GGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGATTTACCTGGGGGATATCGCAGGTCGG                                                                                              >  SRR4302540.6489674/1‑94 (MQ=60)
   CTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGC                                                                                            <  SRR4302540.5383198/94‑1 (MQ=60)
     GTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGA                                                                                          <  SRR4302540.7514848/94‑1 (MQ=60)
     GTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGA                                                                                          >  SRR4302540.6753861/1‑94 (MQ=60)
     GTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGA                                                                                          <  SRR4302540.4459781/94‑1 (MQ=60)
      TCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAG                                                                                         <  SRR4302540.2134895/94‑1 (MQ=60)
      TCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAG                                                                                         <  SRR4302540.5979017/94‑1 (MQ=60)
        AGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTC                                                                                       >  SRR4302540.9311700/1‑94 (MQ=60)
         GAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCC                                                                                      <  SRR4302540.7596493/94‑1 (MQ=60)
          AAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCC                                                                                     >  SRR4302540.2383263/1‑94 (MQ=60)
          AAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCC                                                                                     <  SRR4302540.9884580/94‑1 (MQ=60)
           AAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTCCCTGGGGGATATCGCAGGTCGGGCGAGTCCCA                                                                                    <  SRR4302540.9178813/94‑1 (MQ=60)
             GTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAAT                                                                                  <  SRR4302540.1970030/94‑1 (MQ=60)
             GTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAAT                                                                                  <  SRR4302540.4430994/94‑1 (MQ=60)
              TTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATA                                                                                 >  SRR4302540.9862478/1‑94 (MQ=60)
              TTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATA                                                                                 <  SRR4302540.9905081/94‑1 (MQ=60)
               TTCGCCACCGGTTTACCGTTACACTCTACCAACTTAGTGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATAC                                                                                <  SRR4302540.9681068/94‑1 (MQ=60)
               TTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATAC                                                                                >  SRR4302540.5963683/1‑94 (MQ=60)
               TTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATAC                                                                                <  SRR4302540.2500759/94‑1 (MQ=60)
                 CGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCA                                                                              <  SRR4302540.521478/94‑1 (MQ=60)
                   CCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAA                                                                            >  SRR4302540.5031724/1‑94 (MQ=60)
                   CCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAA                                                                            >  SRR4302540.4389864/1‑94 (MQ=60)
                   CCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAA                                                                            >  SRR4302540.4389863/1‑94 (MQ=60)
                     ACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAAC                                                                          >  SRR4302540.6390077/1‑94 (MQ=60)
                     ACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAAC                                                                          >  SRR4302540.4562423/1‑94 (MQ=60)
                     ACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAAC                                                                          <  SRR4302540.3930288/94‑1 (MQ=60)
                      CCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACT                                                                         <  SRR4302540.8256588/94‑1 (MQ=60)
                       CGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGACCCCATACCAAAACTT                                                                        >  SRR4302540.3365824/1‑94 (MQ=60)
                       CGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGACCCAATACCAAAACTT                                                                        >  SRR4302540.3365825/1‑94 (MQ=60)
                        GGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTA                                                                       >  SRR4302540.9404575/1‑94 (MQ=60)
                        GGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTA                                                                       >  SRR4302540.8299242/1‑94 (MQ=60)
                        GGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTA                                                                       >  SRR4302540.7264013/1‑94 (MQ=60)
                        GGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTA                                                                       >  SRR4302540.908220/1‑94 (MQ=60)
                        GGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTA                                                                       >  SRR4302540.9958628/1‑94 (MQ=60)
                         GTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCTGGTCGGGCGAGTCCCAATACCAAAACTTAA                                                                      >  SRR4302540.9365093/1‑94 (MQ=60)
                          TTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAAT                                                                     <  SRR4302540.1629215/94‑1 (MQ=60)
                          TTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAAT                                                                     >  SRR4302540.4695795/1‑94 (MQ=60)
                          TTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAAT                                                                     >  SRR4302540.4424539/1‑94 (MQ=60)
                             ACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGC                                                                  <  SRR4302540.9415851/94‑1 (MQ=60)
                              CCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCA                                                                 <  SRR4302540.5845122/94‑1 (MQ=60)
                              CCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCA                                                                 <  SRR4302540.6586031/94‑1 (MQ=60)
                              CCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCA                                                                 <  SRR4302540.4355293/94‑1 (MQ=60)
                               CGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAC                                                                <  SRR4302540.9755362/94‑1 (MQ=60)
                                GTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACG                                                               >  SRR4302540.1079623/1‑94 (MQ=60)
                                 TTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              >  SRR4302540.1084581/1‑94 (MQ=60)
                                 TTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              <  SRR4302540.7707103/94‑1 (MQ=60)
                                 TTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              <  SRR4302540.7707104/94‑1 (MQ=60)
                                 TTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              <  SRR4302540.7589235/94‑1 (MQ=60)
                                 TTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              >  SRR4302540.2188240/1‑94 (MQ=60)
                                 TTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              <  SRR4302540.5097061/94‑1 (MQ=60)
                                 TTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              <  SRR4302540.5976334/94‑1 (MQ=60)
                                 TTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              <  SRR4302540.4661644/94‑1 (MQ=60)
                                 GTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              <  SRR4302540.7589239/94‑1 (MQ=60)
                                  TACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCG                                                             >  SRR4302540.2905549/1‑94 (MQ=60)
                                    CACTCTACCAACTTAATGACAATATTCAGGGGTTCTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGG                                                           <  SRR4302540.6285178/94‑1 (MQ=60)
                                    CACTCTACCAACTTAATGACAATATTCAGGGGTTATACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGG                                                           <  SRR4302540.6285179/94‑1 (MQ=60)
                                     ACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGA                                                          >  SRR4302540.8430168/1‑94 (MQ=60)
                                     ACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGA                                                          <  SRR4302540.5461569/94‑1 (MQ=60)
                                     ACTCTACCAACTAAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGA                                                          <  SRR4302540.9248694/94‑1 (MQ=60)
                                      CTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAA                                                         >  SRR4302540.2623721/1‑94 (MQ=60)
                                        CTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGA                                                       >  SRR4302540.7971306/1‑94 (MQ=60)
                                         TACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAG                                                      >  SRR4302540.9514704/1‑94 (MQ=60)
                                          ACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGA                                                     <  SRR4302540.4320955/94‑1 (MQ=60)
                                          ACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGA                                                     <  SRR4302540.7724247/94‑1 (MQ=60)
                                           CCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAA                                                    <  SRR4302540.1847916/94‑1 (MQ=60)
                                           CCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAA                                                    >  SRR4302540.2953395/1‑94 (MQ=60)
                                           CCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAA                                                    >  SRR4302540.7407919/1‑94 (MQ=60)
                                           CCAACGTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAA                                                    <  SRR4302540.1847915/94‑1 (MQ=60)
                                            CAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAG                                                   >  SRR4302540.2670297/1‑94 (MQ=60)
                                             AACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGT                                                  <  SRR4302540.9552889/94‑1 (MQ=60)
                                             AACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACACAAACTTAATTGCACGCGGGAAGACAAGT                                                  >  SRR4302540.508807/1‑94 (MQ=60)
                                             AACTTAATGACAATATTCAGGGGTTTTACCTAGGGGATATCGCAGGACGGCCTAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAACT                                                  >  SRR4302540.2075325/1‑94 (MQ=60)
                                              ACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTG                                                 <  SRR4302540.9247011/94‑1 (MQ=60)
                                               CTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGGGTCCCGATACCAAACGTTACTTGCACGCGGGAAGAGGAGAGG                                                >  SRR4302540.2184095/1‑94 (MQ=60)
                                               CTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGG                                                >  SRR4302540.8812906/1‑94 (MQ=60)
                                               CTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGG                                                <  SRR4302540.5882595/94‑1 (MQ=60)
                                               CTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGG                                                <  SRR4302540.870946/94‑1 (MQ=60)
                                               CTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGG                                                >  SRR4302540.2184094/1‑94 (MQ=60)
                                                TTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGC                                               >  SRR4302540.8231563/1‑94 (MQ=60)
                                                TTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGC                                               >  SRR4302540.6058992/1‑94 (MQ=60)
                                                 TAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCG                                              <  SRR4302540.6494516/94‑1 (MQ=60)
                                                 TAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCG                                              >  SRR4302540.2054759/1‑94 (MQ=60)
                                                 TAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCG                                              <  SRR4302540.6149795/94‑1 (MQ=60)
                                                 TAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGCCCCAATACCAAAACTTAATTGCACGGGGGAAGAGAAGTGGCG                                              >  SRR4302540.7448299/1‑94 (MQ=60)
                                                  AATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGCAGTGGCGG                                             >  SRR4302540.69492/1‑94 (MQ=60)
                                                   ATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGT                                            >  SRR4302540.1826984/1‑94 (MQ=60)
                                                   ATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGC                                            >  SRR4302540.1826983/1‑94 (MQ=60)
                                                    TGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTA                                           >  SRR4302540.9460677/1‑94 (MQ=60)
                                                     GACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTAT                                          <  SRR4302540.5697102/94‑1 (MQ=60)
                                                     GACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTAT                                          <  SRR4302540.3961516/94‑1 (MQ=60)
                                                      ACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATA                                         <  SRR4302540.3899865/94‑1 (MQ=60)
                                                      ACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATA                                         <  SRR4302540.3938285/94‑1 (MQ=60)
                                                      ACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATA                                         <  SRR4302540.756200/94‑1 (MQ=60)
                                                           ATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCA                                    <  SRR4302540.166024/94‑1 (MQ=60)
                                                            TTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAA                                   >  SRR4302540.9324650/1‑94 (MQ=60)
                                                            TTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAA                                   >  SRR4302540.4708524/1‑94 (MQ=60)
                                                            TTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATAGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAA                                   >  SRR4302540.9839314/1‑94 (MQ=60)
                                                             TCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAC                                  >  SRR4302540.5345239/1‑94 (MQ=60)
                                                              CAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACC                                 <  SRR4302540.4393475/94‑1 (MQ=60)
                                                              CAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACC                                 >  SRR4302540.4064209/1‑94 (MQ=60)
                                                               AGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCG                                >  SRR4302540.3549182/1‑94 (MQ=60)
                                                                  GTTTTTACCTGGGGGATATGCAAGGTC‑GGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTT                            <  SRR4302540.8274329/94‑1 (MQ=60)
                                                                   GTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTT                            >  SRR4302540.4415482/1‑94 (MQ=60)
                                                                   GTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTT                            <  SRR4302540.8274328/94‑1 (MQ=60)
                                                                   GTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTACACCGCTTT                            >  SRR4302540.6102232/1‑94 (MQ=60)
                                                                     TTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCTCTTTGC                          >  SRR4302540.3386781/1‑94 (MQ=60)
                                                                     TTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGC                          >  SRR4302540.8204833/1‑94 (MQ=60)
                                                                      TTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCG                         >  SRR4302540.8347784/1‑94 (MQ=60)
                                                                        ACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTA                       <  SRR4302540.5789910/94‑1 (MQ=60)
                                                                         CCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAA                      >  SRR4302540.8563124/1‑94 (MQ=60)
                                                                         CCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAA                      <  SRR4302540.1858569/94‑1 (MQ=60)
                                                                         CCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAA                      >  SRR4302540.5589337/1‑94 (MQ=60)
                                                                          CTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAA                     >  SRR4302540.10138134/1‑94 (MQ=60)
                                                                          CTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAA                     >  SRR4302540.1816615/1‑94 (MQ=60)
                                                                           TGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAAT                    >  SRR4302540.7727111/1‑94 (MQ=60)
                                                                           TGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAAT                    <  SRR4302540.9854192/94‑1 (MQ=60)
                                                                           TGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAAT                    <  SRR4302540.7432789/94‑1 (MQ=60)
                                                                            GGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATC                   <  SRR4302540.827947/94‑1 (MQ=60)
                                                                            GGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATC                   >  SRR4302540.9171284/1‑94 (MQ=60)
                                                                             GGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCC                  <  SRR4302540.942313/94‑1 (MQ=60)
                                                                             GGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCC                  >  SRR4302540.1357105/1‑94 (MQ=60)
                                                                              GGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCA                 <  SRR4302540.2167850/94‑1 (MQ=60)
                                                                              GGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCA                 <  SRR4302540.2167851/94‑1 (MQ=60)
                                                                               GGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAG                >  SRR4302540.7466494/1‑94 (MQ=60)
                                                                               GGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAG                <  SRR4302540.5731001/94‑1 (MQ=60)
                                                                               GGATATCGCAGGGCGGGCGAGTCCCAATACCAAACCTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAG                <  SRR4302540.2955230/94‑1 (MQ=60)
                                                                                GATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGA               <  SRR4302540.6444441/94‑1 (MQ=60)
                                                                                GATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGA               <  SRR4302540.6933256/94‑1 (MQ=60)
                                                                                 ATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGAT              >  SRR4302540.1050620/1‑94 (MQ=60)
                                                                                 ATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGATTTGCGTAAATCCAGAT              >  SRR4302540.8073771/1‑94 (MQ=60)
                                                                                  TATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATT             <  SRR4302540.9133946/94‑1 (MQ=60)
                                                                                   ATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTG            >  SRR4302540.117081/1‑94 (MQ=60)
                                                                                   ATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTG            >  SRR4302540.554583/1‑94 (MQ=60)
                                                                                   ATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTG            >  SRR4302540.2357782/1‑94 (MQ=60)
                                                                                     CGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTC          >  SRR4302540.8319598/1‑94 (MQ=60)
                                                                                      GCAGGTCGGGCGAGTCCC‑ATACCAAAACTTCATTGGAAGCGGGAAGAGAAGTGGCGGgataactttaacggtttgcgtcaaatcagattgtcaa        >  SRR4302540.5706178/1‑57 (MQ=24)
                                                                                       CAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAG        >  SRR4302540.3323374/1‑94 (MQ=60)
                                                                                        AGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGA       >  SRR4302540.1831532/1‑94 (MQ=60)
                                                                                        AGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGA       >  SRR4302540.4220032/1‑94 (MQ=60)
                                                                                        AGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGA       >  SRR4302540.272967/1‑94 (MQ=60)
                                                                                        AGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGA       <  SRR4302540.5776646/94‑1 (MQ=60)
                                                                                         GGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG      >  SRR4302540.369028/1‑94 (MQ=60)
                                                                                         GGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG      >  SRR4302540.4042378/1‑94 (MQ=60)
                                                                                          GTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGA     >  SRR4302540.9715550/1‑94 (MQ=60)
                                                                                           TCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAA    <  SRR4302540.9760347/94‑1 (MQ=60)
                                                                                             GGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGGTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  >  SRR4302540.1004675/1‑94 (MQ=60)
                                                                                             GGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  <  SRR4302540.1811854/94‑1 (MQ=60)
                                                                                             GGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  >  SRR4302540.8875111/1‑94 (MQ=60)
                                                                                             GGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  <  SRR4302540.6036974/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  >  NC_000913/988462‑988648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑