Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 988,555 A→G L342P (CTG→CCG)  pncB ← nicotinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913988,5550AG100.0% 578.5 / NA 181L342P (CTG→CCG) pncBnicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (92/89);  total (92/89)

Only 100 of 190 total aligned reads displayed.

CTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG  >  NC_000913/988465‑988644
                                                                                          |                                                                                         
gtGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCggg                                                                                         <  2:3069473/92‑1 (MQ=255)
cTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCggg                                                                                         <  1:2956559/93‑1 (MQ=255)
 tGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGc                                                                                        >  2:5941416/1‑93 (MQ=255)
  gTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCg                                                                                       <  1:1606773/93‑1 (MQ=255)
   tCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGa                                                                                      <  1:2327835/93‑1 (MQ=255)
    cAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAg                                                                                     <  1:3783723/93‑1 (MQ=255)
    cAGAAAGTTTCGCCACCGGATTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAg                                                                                     >  2:1721826/1‑93 (MQ=255)
     aGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGt                                                                                    >  2:66579/1‑93 (MQ=255)
     aGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGt                                                                                    >  1:314655/1‑93 (MQ=255)
     aGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGt                                                                                    >  1:342316/1‑93 (MQ=255)
        aaGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTccc                                                                                 <  2:2712927/93‑1 (MQ=255)
          gTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCaa                                                                               <  2:5674213/93‑1 (MQ=255)
          gTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCaa                                                                               >  2:1825755/1‑93 (MQ=255)
           tttCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAAt                                                                              <  2:2348228/93‑1 (MQ=255)
           tttCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAAt                                                                              <  2:1183105/93‑1 (MQ=255)
           tttCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAAt                                                                              <  1:1456895/93‑1 (MQ=255)
             tCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATAc                                                                            >  2:5565224/1‑93 (MQ=255)
             tCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATAc                                                                            >  2:48227/1‑93 (MQ=255)
              cGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATAcc                                                                           <  2:5530167/93‑1 (MQ=255)
                ccACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaa                                                                         >  1:5778166/1‑93 (MQ=255)
                  aCCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       <  1:881443/93‑1 (MQ=255)
                  aCCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       >  2:5243143/1‑93 (MQ=255)
                  aCCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       <  1:5210877/93‑1 (MQ=255)
                  aCCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       >  2:3873352/1‑93 (MQ=255)
                  aCCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       >  2:4082019/1‑93 (MQ=255)
                         tACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAAtt                                                                <  1:2118742/93‑1 (MQ=255)
                            cGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCa                                                             <  2:1408443/93‑1 (MQ=255)
                            cGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCa                                                             <  2:3523736/93‑1 (MQ=255)
                             gTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAc                                                            >  2:1442344/1‑93 (MQ=255)
                              ttACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcg                                                           <  1:1768180/93‑1 (MQ=255)
                              ttACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcg                                                           >  2:3578131/1‑93 (MQ=255)
                                acacTCTACCAACTTAATGACAATATTCAGGTGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcgcg                                                         <  2:2040240/93‑1 (MQ=255)
                                 cacTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCgg                                                        <  2:5755160/93‑1 (MQ=255)
                                   ctctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGa                                                      <  1:2703742/93‑1 (MQ=255)
                                    tctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGTGGGaa                                                     <  2:4785979/93‑1 (MQ=255)
                                    tctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGaa                                                     <  1:5322979/93‑1 (MQ=255)
                                      tACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAaga                                                   <  2:5578413/93‑1 (MQ=255)
                                      tACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAaga                                                   >  1:6003245/1‑93 (MQ=255)
                                       aCCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAagag                                                  <  1:1683888/93‑1 (MQ=255)
                                        ccAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAgaga                                                 <  1:3886228/93‑1 (MQ=255)
                                        ccAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAgaga                                                 >  1:4596691/1‑93 (MQ=255)
                                         cAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGaa                                                <  2:3817766/93‑1 (MQ=255)
                                          aaCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAg                                               <  1:1927502/93‑1 (MQ=255)
                                           aattAATGACAATATTCAGGGGGTTTACCTGGGGGATATCGCAGGTCGGGCGAGGCCCAATACCAAAAACTAATTGCACGCGGGAAgaggagg                                              >  2:5880567/3‑92 (MQ=255)
                                           aCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAgg                                              >  2:4915842/1‑92 (MQ=255)
                                           aCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAga                                              >  2:2789071/1‑92 (MQ=255)
                                           aCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGt                                              >  1:3244556/1‑93 (MQ=255)
                                           aCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGt                                              >  2:3986387/1‑93 (MQ=255)
                                            cTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTg                                             >  1:339403/1‑93 (MQ=255)
                                             ttAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTgg                                            <  1:426682/93‑1 (MQ=255)
                                             ttAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTgg                                            <  2:3560722/93‑1 (MQ=255)
                                               aaTGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCg                                          >  1:2170936/1‑93 (MQ=255)
                                                aTGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCgg                                         <  2:3316659/93‑1 (MQ=255)
                                                aTGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCgg                                         >  2:5048282/1‑93 (MQ=255)
                                                  gACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGta                                       <  1:1878779/93‑1 (MQ=255)
                                                  gACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGta                                       <  2:6178313/93‑1 (MQ=255)
                                                  gACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGta                                       >  2:102996/1‑93 (MQ=255)
                                                   aCAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGtat                                      <  2:4874275/93‑1 (MQ=255)
                                                    cAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGtata                                     <  2:1496411/93‑1 (MQ=255)
                                                      atatTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGc                                   >  1:833433/1‑93 (MQ=255)
                                                        atTCAGGGGTTTTACCTGGGTGATAGCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCATGCGGGAAGAGAAGTGGCGGTATAGCTc                                 <  1:1537720/93‑1 (MQ=255)
                                                        atTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTc                                 <  1:6161410/93‑1 (MQ=255)
                                                        atTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTc                                 <  2:4165193/93‑1 (MQ=255)
                                                         ttCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCa                                >  1:5009585/1‑93 (MQ=255)
                                                           cAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAc                              >  1:3500641/1‑93 (MQ=255)
                                                           cAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAc                              <  1:3642401/93‑1 (MQ=255)
                                                           cAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAc                              >  2:253693/1‑93 (MQ=255)
                                                           cAGGGGTGTTACCTGGGTGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAc                              <  1:3117583/93‑1 (MQ=255)
                                                            aGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAcc                             >  2:2130995/1‑93 (MQ=255)
                                                             ggggTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCg                            >  1:420655/1‑93 (MQ=255)
                                                             ggggTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCg                            >  2:1572651/1‑93 (MQ=255)
                                                                gTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                         <  1:5955520/93‑1 (MQ=255)
                                                                gTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                         >  1:2013235/1‑93 (MQ=255)
                                                                gTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                         >  2:5181144/1‑93 (MQ=255)
                                                                 ttttACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCttt                        <  1:3190752/93‑1 (MQ=255)
                                                                 ttttACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCttt                        <  2:5417583/93‑1 (MQ=255)
                                                                 ttttACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCttt                        <  2:196849/93‑1 (MQ=255)
                                                                    tACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCg                     >  2:5575135/1‑93 (MQ=255)
                                                                     aCCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGt                    >  1:2059275/1‑93 (MQ=255)
                                                                     aCCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGt                    >  1:3947313/1‑93 (MQ=255)
                                                                       cTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTaa                  >  1:2464656/1‑93 (MQ=255)
                                                                          ggggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATTGCTCAACCGCTTTGCGTAAATc               >  1:4755526/1‑93 (MQ=255)
                                                                          ggggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATc               >  1:1172850/1‑93 (MQ=255)
                                                                           gggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATcc              >  2:758103/1‑93 (MQ=255)
                                                                            ggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCa             <  2:2946239/93‑1 (MQ=255)
                                                                             gATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAg            <  1:2880523/93‑1 (MQ=255)
                                                                              atatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGa           <  2:4627685/93‑1 (MQ=255)
                                                                              atatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGa           >  2:5208088/1‑93 (MQ=255)
                                                                              atatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGa           <  1:985592/93‑1 (MQ=255)
                                                                               tatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGAt          >  1:2930062/1‑93 (MQ=255)
                                                                                 tCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTg        <  2:3640119/93‑1 (MQ=255)
                                                                                   gCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATGCAGAATGTc      >  1:6056062/1‑93 (MQ=255)
                                                                                   gCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTc      <  2:242381/93‑1 (MQ=255)
                                                                                    cAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCa     <  1:5483890/93‑1 (MQ=255)
                                                                                    cAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCa     <  1:4891616/93‑1 (MQ=255)
                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTTTCaga   >  2:30989/1‑93 (MQ=255)
                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   <  2:1267141/93‑1 (MQ=255)
                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   <  1:5539518/93‑1 (MQ=255)
                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   >  1:108977/1‑93 (MQ=255)
                                                                                       gTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCagag  >  2:4245053/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG  >  NC_000913/988465‑988644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  >  NC_000913/988462‑988648
                                                                                             |                                                                                             
GGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCG                                                                                               <  SRR4302533.5827291/94‑1 (MQ=60)
GGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCG                                                                                               >  SRR4302533.1536367/1‑94 (MQ=60)
  GCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGG                                                                                             <  SRR4302533.101728/94‑1 (MQ=60)
  GCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGG                                                                                             <  SRR4302533.822672/94‑1 (MQ=60)
  GCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGG                                                                                             <  SRR4302533.1549409/94‑1 (MQ=60)
   CTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGC                                                                                            <  SRR4302533.2974632/94‑1 (MQ=60)
     GTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGA                                                                                          <  SRR4302533.1616524/94‑1 (MQ=60)
      TCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAG                                                                                         <  SRR4302533.2342244/94‑1 (MQ=60)
       CAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGT                                                                                        >  SRR4302533.316576/1‑94 (MQ=60)
       CAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGT                                                                                        <  SRR4302533.3806892/94‑1 (MQ=60)
       CAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGT                                                                                        >  SRR4302533.344405/1‑94 (MQ=60)
        AGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGGTTTACCTGGGGGATATCGCAGGTCGGGCGAGTC                                                                                       >  SRR4302533.227041/1‑94 (MQ=60)
         GAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCC                                                                                      >  SRR4302533.2457150/1‑94 (MQ=60)
              TTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATA                                                                                 <  SRR4302533.1465681/94‑1 (MQ=60)
                 CGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCA                                                                              >  SRR4302533.5551677/1‑94 (MQ=60)
                  GCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAA                                                                             >  SRR4302533.5813067/1‑94 (MQ=60)
                   CCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAA                                                                            >  SRR4302533.5651095/1‑94 (MQ=60)
                     ACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAAC                                                                          <  SRR4302533.5242262/94‑1 (MQ=60)
                     ACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAAC                                                                          <  SRR4302533.886621/94‑1 (MQ=60)
                       CGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGTGGTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTT                                                                        >  SRR4302533.5477771/1‑94 (MQ=60)
                       CGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTT                                                                        >  SRR4302533.3222770/1‑94 (MQ=60)
                            TACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTG                                                                   <  SRR4302533.2131811/94‑1 (MQ=60)
                                GTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACG                                                               <  SRR4302533.3437510/94‑1 (MQ=60)
                                 TTACACTCTACCAACTTAATGACAATCTTCAGGGGTTTTAGCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              <  SRR4302533.1778947/94‑1 (MQ=60)
                                 TTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGC                                                              <  SRR4302533.1778946/94‑1 (MQ=60)
                                   ACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGTGG                                                            <  SRR4302533.504059/94‑1 (MQ=60)
                                   ACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGG                                                            >  SRR4302533.1527756/1‑94 (MQ=60)
                                    CACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGG                                                           >  SRR4302533.1576974/1‑94 (MQ=60)
                                      CTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAA                                                         <  SRR4302533.2720326/94‑1 (MQ=60)
                                       TCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAG                                                        <  SRR4302533.5354877/94‑1 (MQ=60)
                                        CTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGA                                                       >  SRR4302533.3308459/1‑94 (MQ=60)
                                        CTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGA                                                       >  SRR4302533.6039621/1‑94 (MQ=60)
                                        CTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGA                                                       <  SRR4302533.1689930/94‑1 (MQ=60)
                                         TACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAG                                                      >  SRR4302533.194849/1‑94 (MQ=60)
                                          ACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGA                                                     >  SRR4302533.4624537/1‑94 (MQ=60)
                                          ACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGA                                                     <  SRR4302533.1694089/94‑1 (MQ=60)
                                           CCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAA                                                    <  SRR4302533.3909936/94‑1 (MQ=60)
                                           CCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAA                                                    >  SRR4302533.171814/1‑94 (MQ=60)
                                            CAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAG                                                   >  SRR4302533.2135172/1‑94 (MQ=60)
                                             AACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGT                                                  >  SRR4302533.3264556/1‑94 (MQ=60)
                                             AACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGT                                                  <  SRR4302533.1939263/94‑1 (MQ=60)
                                              ACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTG                                                 >  SRR4302533.341473/1‑94 (MQ=60)
                                                TTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGC                                               <  SRR4302533.5993786/94‑1 (MQ=60)
                                                TTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGC                                               <  SRR4302533.429228/94‑1 (MQ=60)
                                                 TAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCG                                              <  SRR4302533.4554980/94‑1 (MQ=60)
                                                 TAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCG                                              >  SRR4302533.2184287/1‑94 (MQ=60)
                                                    TGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTA                                           >  SRR4302533.3151952/1‑94 (MQ=60)
                                                     GACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTAT                                          <  SRR4302533.1890229/94‑1 (MQ=60)
                                                     GACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTAT                                          >  SRR4302533.5251197/1‑94 (MQ=60)
                                                       CAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAG                                        >  SRR4302533.47580/1‑94 (MQ=60)
                                                        AATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGC                                       >  SRR4302533.838316/1‑94 (MQ=60)
                                                        AATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGC                                       >  SRR4302533.1923519/1‑94 (MQ=60)
                                                         ATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCT                                      >  SRR4302533.854373/1‑94 (MQ=60)
                                                           ATTCAGGGGTTTTACCTGGGTGATAGCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCATGCGGGAAGAGAAGTGGCGGTATAGCTCA                                    <  SRR4302533.1546959/94‑1 (MQ=60)
                                                           ATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCA                                    >  SRR4302533.6171666/1‑94 (MQ=60)
                                                           ATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCA                                    <  SRR4302533.2854697/94‑1 (MQ=60)
                                                           ATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCA                                    >  SRR4302533.5039934/1‑94 (MQ=60)
                                                           ATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCA                                    <  SRR4302533.6198462/94‑1 (MQ=60)
                                                            TTCATGGGTTTTACCTGGGGGATATCGCAAGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGCAGAGAAGTGGCGGTCTAGCTCAA                                   <  SRR4302533.972261/94‑1 (MQ=60)
                                                             TCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAC                                  >  SRR4302533.3522228/1‑94 (MQ=60)
                                                              CAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACC                                 <  SRR4302533.3664797/94‑1 (MQ=60)
                                                              CAGGGGTGTTACCTGGGTGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACC                                 <  SRR4302533.3136750/94‑1 (MQ=60)
                                                               AGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCG                                >  SRR4302533.2902657/1‑94 (MQ=60)
                                                               AGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCG                                >  SRR4302533.423172/1‑94 (MQ=60)
                                                               AGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCG                                >  SRR4302533.5037065/1‑94 (MQ=60)
                                                                 GGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCT                              <  SRR4302533.2966526/94‑1 (MQ=60)
                                                                  GGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTT                             >  SRR4302533.666145/1‑94 (MQ=60)
                                                                  GGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTT                             >  SRR4302533.2025636/1‑94 (MQ=60)
                                                                   GTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTT                            <  SRR4302533.3931098/94‑1 (MQ=60)
                                                                   GTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTT                            <  SRR4302533.5991546/94‑1 (MQ=60)
                                                                    TTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTG                           <  SRR4302533.3210381/94‑1 (MQ=60)
                                                                    TTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTG                           <  SRR4302533.3778496/94‑1 (MQ=60)
                                                                     TTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGC                          <  SRR4302533.3042352/94‑1 (MQ=60)
                                                                       TACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGT                        >  SRR4302533.3971385/1‑94 (MQ=60)
                                                                       TACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGT                        >  SRR4302533.2071973/1‑94 (MQ=60)
                                                                         CCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAA                      >  SRR4302533.3445955/1‑94 (MQ=60)
                                                                         CCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAA                      >  SRR4302533.2598556/1‑94 (MQ=60)
                                                                         CCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAA                      >  SRR4302533.2479857/1‑94 (MQ=60)
                                                                           TGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGCATAGCTCAACCGCTTTGCGTAAAT                    >  SRR4302533.1460654/1‑94 (MQ=60)
                                                                            GGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATTGCTCAACCGCTTTGCGTAAATC                   >  SRR4302533.4784383/1‑94 (MQ=60)
                                                                            GGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATC                   >  SRR4302533.1179890/1‑94 (MQ=60)
                                                                              GGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCA                 <  SRR4302533.3528663/94‑1 (MQ=60)
                                                                                GATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGA               <  SRR4302533.2898244/94‑1 (MQ=60)
                                                                                 ATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGAT              <  SRR4302533.991362/94‑1 (MQ=60)
                                                                                 ATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGAT              <  SRR4302533.3871097/94‑1 (MQ=60)
                                                                                 ATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGAT              >  SRR4302533.2947984/1‑94 (MQ=60)
                                                                                     CGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATGCAGAATGTC          >  SRR4302533.6092735/1‑94 (MQ=60)
                                                                                     CGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTC          >  SRR4302533.6092734/1‑94 (MQ=60)
                                                                                       CAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAG        <  SRR4302533.5516943/94‑1 (MQ=60)
                                                                                       CAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAG        <  SRR4302533.4921255/94‑1 (MQ=60)
                                                                                       CAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAG        <  SRR4302533.4877185/94‑1 (MQ=60)
                                                                                        AGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGA       >  SRR4302533.109671/1‑94 (MQ=60)
                                                                                         GGTCGGGCGAGTCCCAATACCAAAACTTCATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG      <  SRR4302533.4001680/94‑1 (MQ=60)
                                                                                         GGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG      <  SRR4302533.5572969/94‑1 (MQ=60)
                                                                                         GGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG      >  SRR4302533.2035611/1‑94 (MQ=60)
                                                                                          GTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTGAGAGA     >  SRR4302533.6248478/1‑94 (MQ=60)
                                                                                            CGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAA   >  SRR4302533.1578078/1‑94 (MQ=60)
                                                                                             GGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  <  SRR4302533.6333368/94‑1 (MQ=60)
                                                                                             GGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  >  SRR4302533.3545614/1‑94 (MQ=60)
                                                                                             GGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  <  SRR4302533.116622/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAA  >  NC_000913/988462‑988648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑