Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	998,433	A→G	intergenic (+19/‑6)	ycbR → / → ycbS	putative chaperone/putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	998,433	0	A	G	99.1%	667.8 / NA	210	intergenic (+19/‑6)	ycbR/ycbS	putative chaperone/putative outer membrane protein
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (122/86); total (124/86)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

ATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTC  >  NC_000913/998344‑998521

aTAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCg                                                                                       >  1:6692749/1‑93 (MQ=255)

 tAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCga                                                                                      >  1:9348988/1‑93 (MQ=255)

   gcagcGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAAATGTAAATCTTAAGCCGTCTTCAG‑TTAAGAGGGCgagt                                                                                    >  1:1254620/1‑92 (MQ=255)

    cagcGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGAt                                                                                   <  1:9343761/93‑1 (MQ=255)

     agcGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATg                                                                                  >  1:9850054/1‑93 (MQ=255)

     agcGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATg                                                                                  <  2:3189091/93‑1 (MQ=255)

        gTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGtat                                                                               >  2:1787883/1‑93 (MQ=255)

        gTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGtat                                                                               <  2:7653493/93‑1 (MQ=255)

         tAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCTG‑CTAAGAGGGCGAGATGtata                                                                              >  1:2128463/1‑93 (MQ=255)

         tAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGtata                                                                              <  1:2058003/93‑1 (MQ=255)

          aaCGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAg                                                                             <  2:8594752/93‑1 (MQ=255)

          aaCGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAg                                                                             <  2:2097758/93‑1 (MQ=255)

          aaCGTATCGAACAATCAATGCCTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAg                                                                             <  2:2572772/93‑1 (MQ=255)

           aCGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGa                                                                            >  2:8379766/1‑93 (MQ=255)

           aCGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGa                                                                            >  2:8345128/1‑93 (MQ=255)

             gTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAACTCGTATGCCGTCTTCAG‑CTTAGAGGGCGAGATGTATAGAAc                                                                          >  1:7816224/1‑93 (MQ=255)

             gTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAAc                                                                          >  1:8271621/1‑93 (MQ=255)

              tATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACt                                                                         >  1:9901194/1‑93 (MQ=255)

                tCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCa                                                                       >  2:2285371/1‑93 (MQ=255)

                  gAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATc                                                                     <  2:8185957/93‑1 (MQ=255)

                  caaCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATc                                                                     <  1:4788928/92‑1 (MQ=255)

                     ccATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTATGCCGTCTTCTGCTTGAAAGA‑CGAGATGTATAGAACTCATCGac                                                                  >  1:1399587/1‑93 (MQ=255)

                     ccATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGac                                                                  >  2:5367301/1‑93 (MQ=255)

                      cATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTATATCGTATGCCGTCTTCTG‑CTTGAAAAAAGAGATGTATAGAACTCATCGaca                                                                 >  1:1072951/1‑93 (MQ=255)

                      cATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGaca                                                                 <  2:4416321/93‑1 (MQ=255)

                      cATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGaca                                                                 >  2:3419888/1‑93 (MQ=255)

                        tCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGacaac                                                               >  2:6971481/1‑93 (MQ=255)

                        tCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGacaac                                                               <  2:7786527/93‑1 (MQ=255)

                        tCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGacaac                                                               >  2:6271033/1‑93 (MQ=255)

                         cAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGacaaca                                                              >  1:8148676/1‑93 (MQ=255)

                         cAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGacaaca                                                              <  1:4090732/93‑1 (MQ=255)

                          aaTGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAacac                                                             >  2:7721268/1‑93 (MQ=255)

                           aTGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAAcaca                                                            >  1:2578676/1‑93 (MQ=255)

                           aTGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAAcaca                                                            >  1:4234721/1‑93 (MQ=255)

                             gACTACGGGGCGGAAACGCCGCAACTCAACTGTATCTCGTATGCCGTCTTCTG‑CTTGAAAAAAGAAATATATAGAACTCATCGATAAGCCAGc                                                          >  1:4481643/1‑93 (MQ=255)

                              aCTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGcc                                                         <  1:9041097/93‑1 (MQ=255)

                               cTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCt                                                        >  2:5297653/1‑93 (MQ=255)

                               cTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCt                                                        >  1:5527231/1‑93 (MQ=255)

                                tACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTg                                                       <  1:9411271/93‑1 (MQ=255)

                                 aCGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGt                                                      <  2:4686935/93‑1 (MQ=255)

                                  cGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGCGATGTATAGAACTCATCGACAACACAGCCTGtt                                                     <  1:8410395/93‑1 (MQ=255)

                                         gAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGctct                                              >  1:6959522/1‑93 (MQ=255)

                                         gAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGctct                                              >  2:3218683/1‑93 (MQ=255)

                                         gAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGctct                                              >  1:9769563/1‑93 (MQ=255)

                                          aaaCGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCtg                                             >  2:4510894/1‑93 (MQ=255)

                                            aCGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCtggt                                           >  2:7025098/1‑93 (MQ=255)

                                            aCGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCtggt                                           <  2:6288196/93‑1 (MQ=255)

                                             cgccgcAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGCGGCAGAGATGTATAGAACGCATCGACAACACAGCCTGTTAAGCTCtggtg                                          <  2:2727911/93‑1 (MQ=255)

                                             cgccgcAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCtggtg                                          <  1:319824/93‑1 (MQ=255)

                                              gccgcAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCtggtgg                                         >  1:6562270/1‑93 (MQ=255)

                                              gccgcAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCtggtgg                                         >  2:3792200/1‑93 (MQ=255)

                                               tcgcAACTCAACTGTAAATCGTACGCAGTCTTCAG‑TTAAGAGGGCGAGATGTATAGACCCCATCGACAACCCAGCATGTTAAGCTCTGGTGGa                                        <  1:9916377/92‑1 (MQ=255)

                                               ccgcAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGa                                        <  2:3743657/93‑1 (MQ=255)

                                                cgcAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAg                                       <  2:4979219/93‑1 (MQ=255)

                                                   aactcaactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGc                                    >  2:5946505/1‑93 (MQ=255)

                                                   aactcaactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGc                                    >  1:9732345/1‑93 (MQ=255)

                                                   aactcaactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGc                                    >  1:7898149/1‑93 (MQ=255)

                                                   aactcaactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGc                                    <  1:8460483/93‑1 (MQ=255)

                                                     ctcaactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCa                                  >  1:6380860/1‑93 (MQ=255)

                                                      tcaactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCAt                                 >  1:7705123/1‑93 (MQ=255)

                                                       caactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATc                                <  2:9791686/93‑1 (MQ=255)

                                                       caactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATc                                >  1:3860501/1‑93 (MQ=255)

                                                       caactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATc                                >  1:2831761/1‑93 (MQ=255)

                                                       caactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATc                                <  2:1683237/93‑1 (MQ=255)

                                                        aactGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCg                               >  2:927826/1‑93 (MQ=255)

                                                         actGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGt                              >  1:432799/1‑93 (MQ=255)

                                                           tGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGttt                            >  2:2656800/1‑93 (MQ=255)

                                                           tGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGttt                            >  1:3628346/1‑93 (MQ=255)

                                                            gTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTa                           >  1:4530643/1‑93 (MQ=255)

                                                            gTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTa                           >  2:8587144/1‑93 (MQ=255)

                                                            gTAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTa                           <  2:8469340/93‑1 (MQ=255)

                                                             tAAATCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTAt                          <  1:1675637/93‑1 (MQ=255)

                                                              aaaTCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTAtt                         >  2:1069302/1‑93 (MQ=255)

                                                              aaaTCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTAtt                         <  2:9157837/93‑1 (MQ=255)

                                                              aaaTCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTAtt                         >  1:88395/1‑93 (MQ=255)

                                                              aaaTCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTAtt                         <  1:4910870/93‑1 (MQ=255)

                                                                aTCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATtgg                       <  2:5048283/93‑1 (MQ=255)

                                                                aTCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATtgg                       >  1:9534206/1‑93 (MQ=255)

                                                                aTCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATtgg                       <  1:1343419/93‑1 (MQ=255)

                                                                 tCGTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATtggt                      >  2:2243801/1‑93 (MQ=255)

                                                                   gTAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATtggtgg                    >  2:3049907/1‑93 (MQ=255)

                                                                    tAAGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTggg                   <  2:1643785/93‑1 (MQ=255)

                                                                      aGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCt                 >  1:587361/1‑93 (MQ=255)

                                                                      aGCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCt                 >  1:6063489/1‑93 (MQ=255)

                                                                       gCCGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCtg                <  1:5202940/93‑1 (MQ=255)

                                                                         cGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCtggt              >  1:2970944/1‑93 (MQ=255)

                                                                         cGTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCtggt              >  1:9660722/1‑93 (MQ=255)

                                                                          gTCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGAGGAGTGCCATCGTTTATTGGTGGGCtggtg             >  1:8830740/1‑93 (MQ=255)

                                                                           tCTTCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCtggtgg            >  2:194032/1‑93 (MQ=255)

                                                                              tCAG‑TTAGGAGGGCGAGATGTATTGATCTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGgtgt         <  2:7281591/93‑1 (MQ=255)

                                                                              tCAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGgtgt         >  2:4141740/1‑93 (MQ=255)

                                                                               cAG‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGtt        >  1:7234902/1‑93 (MQ=255)

                                                                                 g‑TTAAGAGGGCGAGATGTATGGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTtg      >  2:9560848/1‑93 (MQ=255)

                                                                                 g‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTtg      <  2:6697487/93‑1 (MQ=255)

                                                                                   ttAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTtgt     >  1:3826801/1‑93 (MQ=255)

                                                                                    tAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTtgtg    >  2:4844190/1‑93 (MQ=255)

                                                                                     aaGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTgtgt   >  2:1407273/1‑93 (MQ=255)

                                                                                      agagggCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTc  >  2:7943356/1‑93 (MQ=255)

                                                                                      agagggCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTc  >  1:584745/1‑93 (MQ=255)

                                                                                      agagggCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTc  >  2:6475271/1‑93 (MQ=255)

ATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑TTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTC  >  NC_000913/998344‑998521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAAAATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTCGGCA  >  NC_000913/998340‑998525

AAAAATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGG                                                                                              >  SRR4302540.792905/1‑94 (MQ=60)

AAAAATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGG                                                                                              >  SRR4302540.8298233/1‑94 (MQ=60)

 AAAATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGG                                                                                             >  SRR4302540.8011686/1‑94 (MQ=60)

   AATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCG                                                                                           >  SRR4302540.6766639/1‑94 (MQ=60)

    ATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGA                                                                                          >  SRR4302540.9460347/1‑94 (MQ=60)

      AGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGA                                                                                        >  SRR4302540.1267474/1‑94 (MQ=60)

      AGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAAATGTAAATCTTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGT                                                                                        >  SRR4302540.1267473/1‑94 (MQ=60)

      AGCAGCGTAACGAATCAAACCATCAATGACTCCGAGTCGGAAACGCCGCAGCTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGA                                                                                        <  SRR4302540.8363899/94‑1 (MQ=60)

        CAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATG                                                                                      >  SRR4302540.9965187/1‑94 (MQ=60)

        CAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATG                                                                                      <  SRR4302540.9455083/94‑1 (MQ=60)

            GTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTATGCCGTCTTCAG‑‑TTAGGAAGGCGAGATGTATA                                                                                  >  SRR4302540.9919135/1‑94 (MQ=60)

            GTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCTG‑‑CTAGGAGGGCGAGATGTATA                                                                                  >  SRR4302540.9646976/1‑94 (MQ=60)

            GTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCTG‑‑CTAAGAGGGCGAGATGTATA                                                                                  >  SRR4302540.2151300/1‑94 (MQ=60)

             TAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAG                                                                                 >  SRR4302540.3172595/1‑94 (MQ=60)

             TAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAG                                                                                 <  SRR4302540.2080154/94‑1 (MQ=60)

             TAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAG                                                                                 >  SRR4302540.383056/1‑94 (MQ=60)

                CGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAACTCGTATGCCGTCTTCAG‑‑CTTAGAGGGCGAGATGTATAGAAC                                                                              >  SRR4302540.7907572/1‑94 (MQ=60)

                CGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAAC                                                                              >  SRR4302540.8367723/1‑94 (MQ=60)

                CGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGACGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAAT                                                                              >  SRR4302540.8030297/1‑94 (MQ=60)

                 GTATCGAACCATCGATGACTACGGGGCGGAACCGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACT                                                                             <  SRR4302540.9074748/94‑1 (MQ=60)

                 GTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTATGCCGTCTTCTG‑‑CTTGGAAGGCGAGATGTATAGAACT                                                                             >  SRR4302540.460793/1‑94 (MQ=60)

                 GTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACT                                                                             >  SRR4302540.10016866/1‑94 (MQ=60)

                 GTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACT                                                                             >  SRR4302540.10016867/1‑94 (MQ=60)

                 GTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACT                                                                             >  SRR4302540.9673897/1‑94 (MQ=60)

                 GTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACT                                                                             >  SRR4302540.9673896/1‑94 (MQ=60)

                   ATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCA                                                                           >  SRR4302540.1489771/1‑94 (MQ=60)

                      GAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCG                                                                        <  SRR4302540.4840142/94‑1 (MQ=60)

                      CAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCG                                                                        <  SRR4302540.4840141/94‑1 (MQ=60)

                       AACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGA                                                                       <  SRR4302540.5744757/94‑1 (MQ=60)

                        ACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTATGCCGTCTTCTGC‑TTGAAAGA‑CGAGATGTATAGAACTCATCGAC                                                                      >  SRR4302540.1414631/1‑94 (MQ=60)

                         CCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTATATCGTATGCCGTCTTCTGCTTGAAAAAA‑‑GAGATGTATAGAACTCATCGACA                                                                     >  SRR4302540.1083904/1‑94 (MQ=53)

                         CCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACA                                                                     >  SRR4302540.4260756/1‑94 (MQ=60)

                           ATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATaactgaa                                                                   >  SRR4302540.2548614/1‑87 (MQ=60)

                           ATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAAC                                                                   >  SRR4302540.932114/1‑94 (MQ=60)

                            TCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACA                                                                  >  SRR4302540.8243269/1‑94 (MQ=60)

                             CAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACAC                                                                 >  SRR4302540.2282841/1‑94 (MQ=60)

                             CAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACAC                                                                 <  SRR4302540.4134861/94‑1 (MQ=60)

                              AATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACA                                                                >  SRR4302540.2606479/1‑94 (MQ=60)

                              AATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACA                                                                >  SRR4302540.4280801/1‑94 (MQ=60)

                                  ACTACGGGGCGGAAACGCCGCAACTCAACTGTAACTCGTATGCCGTCTTCAG‑‑TTTAGAGGGCGAGCTGTATCGAACTCATCGACAACACAGCCT                                                            >  SRR4302540.7361705/1‑94 (MQ=60)

                                  ACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCT                                                            >  SRR4302540.5588100/1‑94 (MQ=60)

                                  ACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCT                                                            <  SRR4302540.9149623/94‑1 (MQ=60)

                                    TACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGT                                                          <  SRR4302540.9523170/94‑1 (MQ=60)

                                      CGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGCGATGTATAGAACTCATCGACAACACAGCCTGTTA                                                        <  SRR4302540.8508014/94‑1 (MQ=60)

                                      CGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTA                                                        <  SRR4302540.6702704/94‑1 (MQ=60)

                                       GGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAA                                                       <  SRR4302540.99930/94‑1 (MQ=60)

                                         GGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGC                                                     <  SRR4302540.9436792/94‑1 (MQ=60)

                                         GGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGC                                                     >  SRR4302540.9859162/1‑94 (MQ=60)

                                           CGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTC                                                   <  SRR4302540.1257770/94‑1 (MQ=60)

                                            GGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCT                                                  >  SRR4302540.7036940/1‑94 (MQ=60)

                                            GGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCT                                                  >  SRR4302540.9884113/1‑94 (MQ=60)

                                             GAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTG                                                 <  SRR4302540.4491329/94‑1 (MQ=60)

                                                 CGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGG                                             >  SRR4302540.6635008/1‑94 (MQ=60)

                                                 CGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGG                                             <  SRR4302540.323022/94‑1 (MQ=60)

                                                   TCGCAACTCAACTGTAAATCGTACGCAGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGACCCCATCGACAACCCAGCATGTTAAGCTCTGGTGGAG                                           <  SRR4302540.10032187/94‑1 (MQ=60)

                                                    CGCCACTCAACTGTAAATCGGAAGCTGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGT                                          <  SRR4302540.2019582/94‑1 (MQ=60)

                                                      CAACTCAACTGTAAATCGTAGGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAGCCCAGCCTGTCAAGCTCTGGTGGCGTGC                                        >  SRR4302540.9846624/1‑94 (MQ=60)

                                                      CAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGC                                        >  SRR4302540.784629/1‑94 (MQ=60)

                                                      CAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGC                                        >  SRR4302540.7990285/1‑94 (MQ=60)

                                                      CAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGC                                        >  SRR4302540.9846625/1‑94 (MQ=60)

                                                      CAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGC                                        >  SRR4302540.1463464/1‑94 (MQ=60)

                                                       AACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCC                                       >  SRR4302540.4536989/1‑94 (MQ=60)

                                                       AACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCC                                       >  SRR4302540.5192950/1‑94 (MQ=60)

                                                       AACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCC                                       <  SRR4302540.8558715/94‑1 (MQ=60)

                                                        ACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCA                                      >  SRR4302540.5952766/1‑94 (MQ=60)

                                                        ACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCA                                      >  SRR4302540.6451898/1‑94 (MQ=60)

                                                        ACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCA                                      >  SRR4302540.4268004/1‑94 (MQ=60)

                                                         CTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCAT                                     >  SRR4302540.7793372/1‑94 (MQ=60)

                                                         CTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCAT                                     <  SRR4302540.6903809/94‑1 (MQ=60)

                                                          TCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATC                                    >  SRR4302540.10014174/1‑94 (MQ=60)

                                                          TCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATC                                    >  SRR4302540.3902462/1‑94 (MQ=60)

                                                          TCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATC                                    >  SRR4302540.2862388/1‑94 (MQ=60)

                                                          TCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATC                                    <  SRR4302540.8927192/94‑1 (MQ=60)

                                                            AACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGT                                  >  SRR4302540.437376/1‑94 (MQ=60)

                                                              CTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTT                                >  SRR4302540.3668062/1‑94 (MQ=60)

                                                               TGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTA                               >  SRR4302540.4579685/1‑94 (MQ=60)

                                                                GTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTAT                              <  SRR4302540.8495386/94‑1 (MQ=60)

                                                                 TAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATT                             >  SRR4302540.89330/1‑94 (MQ=60)

                                                                 TAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATT                             <  SRR4302540.1693838/94‑1 (MQ=60)

                                                                  AAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTG                            <  SRR4302540.4963744/94‑1 (MQ=60)

                                                                  AAATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTG                            >  SRR4302540.5798944/1‑94 (MQ=60)

                                                                   AATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGG                           <  SRR4302540.2828458/94‑1 (MQ=60)

                                                                   AATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGG                           >  SRR4302540.9647072/1‑94 (MQ=60)

                                                                    ATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGT                          <  SRR4302540.9455503/94‑1 (MQ=60)

                                                                    ATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGT                          <  SRR4302540.1357507/94‑1 (MQ=60)

                                                                    ATCGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGT                          <  SRR4302540.139190/94‑1 (MQ=60)

                                                                    ATCGCAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGT                          <  SRR4302540.5587617/94‑1 (MQ=60)

                                                                      CGTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGG                        >  SRR4302540.8548649/1‑94 (MQ=60)

                                                                       GTAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGG                       >  SRR4302540.3369535/1‑94 (MQ=60)

                                                                        TAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGC                      >  SRR4302540.870989/1‑94 (MQ=60)

                                                                        TAAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGC                      >  SRR4302540.2783735/1‑94 (MQ=60)

                                                                         AAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCT                     >  SRR4302540.6130890/1‑94 (MQ=60)

                                                                         AAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCT                     >  SRR4302540.2887980/1‑94 (MQ=60)

                                                                         AAGCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCT                     >  SRR4302540.593697/1‑94 (MQ=60)

                                                                           GCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGG                   <  SRR4302540.5259606/94‑1 (MQ=60)

                                                                           GCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGG                   <  SRR4302540.3886613/94‑1 (MQ=60)

                                                                           GCCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGG                   <  SRR4302540.833537/94‑1 (MQ=60)

                                                                            CCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGT                  >  SRR4302540.3003757/1‑94 (MQ=60)

                                                                            CCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGT                  >  SRR4302540.9774439/1‑94 (MQ=60)

                                                                            CCGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGT                  <  SRR4302540.6596456/94‑1 (MQ=60)

                                                                             CGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTG                 >  SRR4302540.8932871/1‑94 (MQ=60)

                                                                             CGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTG                 >  SRR4302540.7701534/1‑94 (MQ=60)

                                                                             CGTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGAGGAGTGCCATCGTTTATTGGTGGGCTGGTG                 >  SRR4302540.8932870/1‑94 (MQ=60)

                                                                              GTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGG                >  SRR4302540.9505449/1‑94 (MQ=60)

                                                                              GTCTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGG                >  SRR4302540.378246/1‑94 (MQ=60)

                                                                                CTTCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTG              <  SRR4302540.7996099/94‑1 (MQ=60)

                                                                                  TCAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTT            >  SRR4302540.7315603/1‑94 (MQ=60)

                                                                                   CAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTT           >  SRR4302540.4610831/1‑94 (MQ=60)

                                                                                   CAG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTT           <  SRR4302540.6333286/94‑1 (MQ=60)

                                                                                    AG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTG          >  SRR4302540.4363493/1‑94 (MQ=60)

                                                                                    AG‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGGGGTGTTTG          >  SRR4302540.2094289/1‑94 (MQ=60)

                                                                                     G‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGT         >  SRR4302540.3868445/1‑94 (MQ=60)

                                                                                     G‑‑TTAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGT         >  SRR4302540.79109/1‑94 (MQ=60)

                                                                                         TAAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGCTTGTGT       >  SRR4302540.3949164/1‑94 (MQ=60)

                                                                                          AAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTC      >  SRR4302540.6201135/1‑94 (MQ=60)

                                                                                          AAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTC      >  SRR4302540.591061/1‑94 (MQ=60)

                                                                                          AAGAGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTC      >  SRR4302540.591060/1‑94 (MQ=60)

                                                                                             AGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTCGGC   >  SRR4302540.6544791/1‑94 (MQ=60)

                                                                                             AGGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTCGGC   >  SRR4302540.8809868/1‑94 (MQ=60)

                                                                                              GGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTCGGCA  >  SRR4302540.3942743/1‑94 (MQ=60)

                                                                                              GGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTCGGCA  <  SRR4302540.4986358/94‑1 (MQ=60)

                                                                                              GGGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTCGGCA  >  SRR4302540.8994646/1‑94 (MQ=60)

AAAAATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAG‑‑TTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATCGTTTATTGGTGGGCTGGTGGTGTTTGTGTCGGCA  >  NC_000913/998340‑998525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`