Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 999,571 A→G Q378R (CAG→CGG)  ycbS → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913999,5710AG99.6% 877.1 / ‑5.4 271Q378R (CAG→CGG) ycbSputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (126/144);  total (127/144)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 288 total aligned reads displayed.

CGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACC  >  NC_000913/999481‑999660
                                                                                          |                                                                                         
cGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCggg                                                                                         <  2:5966100/93‑1 (MQ=255)
 gTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGga                                                                                        >  1:50287/1‑93 (MQ=255)
 gTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGga                                                                                        >  1:2090941/1‑93 (MQ=255)
 gTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGga                                                                                        <  1:3498922/93‑1 (MQ=255)
  ttatcCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGgag                                                                                       <  1:9382158/90‑1 (MQ=255)
  ttCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGgag                                                                                       <  1:5278614/93‑1 (MQ=255)
  ttCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGgag                                                                                       >  2:3156390/1‑93 (MQ=255)
   tctcCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGgaga                                                                                      <  1:9022435/93‑1 (MQ=255)
   tctcCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGgaga                                                                                      <  2:4225065/93‑1 (MQ=255)
     tcCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGc                                                                                    <  2:6149366/93‑1 (MQ=255)
     tcCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGc                                                                                    <  2:3690434/93‑1 (MQ=255)
      ccAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCa                                                                                   <  1:6483882/93‑1 (MQ=255)
        aGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGTCCaaa                                                                                 >  1:2835328/1‑93 (MQ=255)
        aGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCaaa                                                                                 <  2:6728120/93‑1 (MQ=255)
         gtgtGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAAt                                                                                >  2:10041031/1‑93 (MQ=255)
           gtgCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAAttg                                                                              >  1:3557868/1‑92 (MQ=255)
               cATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGccc                                                                          <  1:7939503/93‑1 (MQ=255)
                aTTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCa                                                                         <  2:4109355/93‑1 (MQ=255)
                aTTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCa                                                                         >  1:1152815/1‑93 (MQ=255)
                  tACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAgg                                                                       >  1:7047783/1‑93 (MQ=255)
                    cTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGcc                                                                     >  1:7703905/1‑93 (MQ=255)
                    cTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGcc                                                                     <  2:7089300/93‑1 (MQ=255)
                     tCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCa                                                                    <  1:9755271/93‑1 (MQ=255)
                     tCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCa                                                                    >  2:570486/1‑93 (MQ=255)
                     tCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCa                                                                    <  1:8075078/93‑1 (MQ=255)
                     tCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCa                                                                    >  2:4293410/1‑93 (MQ=255)
                      ccAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCAc                                                                   >  2:5088050/1‑93 (MQ=255)
                       cAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACg                                                                  <  1:1604801/93‑1 (MQ=255)
                         gCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGtt                                                                <  2:4701378/93‑1 (MQ=255)
                           gTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGc                                                              <  2:1310755/93‑1 (MQ=255)
                           gTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGc                                                              <  2:5309294/93‑1 (MQ=255)
                           gTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGc                                                              <  1:641429/93‑1 (MQ=255)
                            tCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCa                                                             >  2:250421/1‑93 (MQ=255)
                            tCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCa                                                             <  2:1860427/93‑1 (MQ=255)
                            gcAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCa                                                             <  2:1421059/92‑1 (MQ=255)
                             cAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAg                                                            <  2:9598934/93‑1 (MQ=255)
                             cAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAg                                                            <  1:5192505/93‑1 (MQ=255)
                             cAGGGGCGAATCAAACACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAg                                                            <  2:7612735/93‑1 (MQ=255)
                               ggggCGAATCAAATACGCGGTTACACGGGCGAAATACTGAACCAATAGTAATGAACTGCGGGAGAGCAAATTTGCCCAGGCCATGTTGCAGTg                                                          >  2:8964771/1‑93 (MQ=255)
                               ggggCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTg                                                          >  2:4518713/1‑93 (MQ=255)
                               ggggCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTg                                                          >  2:27405/1‑93 (MQ=255)
                               ggggCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTg                                                          >  1:5497270/1‑93 (MQ=255)
                                gggCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTgg                                                         <  1:609956/93‑1 (MQ=255)
                                  gCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTgggg                                                       <  2:2259704/93‑1 (MQ=255)
                                   cGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTggggg                                                      >  2:1926054/1‑92 (MQ=255)
                                   cGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGc                                                      <  1:5408940/93‑1 (MQ=255)
                                     aaTCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCgg                                                    <  1:2635395/93‑1 (MQ=255)
                                      aTCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGa                                                   >  1:5740628/1‑93 (MQ=255)
                                       tCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGAc                                                  >  1:4303861/1‑93 (MQ=255)
                                       tCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGAc                                                  >  2:7523853/1‑93 (MQ=255)
                                         aaaTACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCg                                                <  1:3625871/93‑1 (MQ=255)
                                           aTACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTg                                              >  2:6275140/1‑93 (MQ=255)
                                             aCGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTggg                                            >  2:7923147/1‑93 (MQ=255)
                                               gcgGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGa                                          <  1:1856649/93‑1 (MQ=255)
                                                  gTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACg                                       >  2:4002923/1‑93 (MQ=255)
                                                  gTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACg                                       >  2:5783899/1‑93 (MQ=255)
                                                     acacTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGAcc                                    >  2:2119467/1‑93 (MQ=255)
                                                      cacTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCt                                   <  1:9126835/93‑1 (MQ=255)
                                                       acTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTg                                  <  2:8855508/93‑1 (MQ=255)
                                                        cTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTgg                                 <  2:5661046/93‑1 (MQ=255)
                                                        cTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTgg                                 <  2:7746152/93‑1 (MQ=255)
                                                        cTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTgg                                 <  2:6424085/93‑1 (MQ=255)
                                                        cTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTgg                                 >  1:2338977/1‑93 (MQ=255)
                                                         tGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCGGTGGGCCGGACCGTGGGGAACGCCATggg                                >  2:8499683/1‑92 (MQ=255)
                                                         tGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGt                                >  2:9135961/1‑93 (MQ=255)
                                                         tGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGt                                <  1:6687894/93‑1 (MQ=255)
                                                             gAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTAtgg                            >  1:3343514/1‑93 (MQ=255)
                                                              aaaTACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTAtggt                           <  2:3317620/93‑1 (MQ=255)
                                                              aaaTACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTAtggt                           <  1:5801342/93‑1 (MQ=255)
                                                              aaaTACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTAtggt                           >  2:9690239/1‑93 (MQ=255)
                                                              aaaTACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTAtggg                           >  1:2947790/1‑92 (MQ=255)
                                                               aaTACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAAGGACCTGGTAtggtg                          >  2:7500075/1‑93 (MQ=255)
                                                               aaTACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTAtggtg                          <  1:3938417/93‑1 (MQ=255)
                                                               aaTACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTCGGGCGGACCGTGGGGAACGACCTGGTAtggtg                          <  1:1115538/93‑1 (MQ=255)
                                                                 tACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTAtggtggt                        <  2:677564/93‑1 (MQ=255)
                                                                    aGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGa                     >  2:4663951/1‑93 (MQ=255)
                                                                    aGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGa                     <  1:9572439/93‑1 (MQ=255)
                                                                     gAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGAc                    >  1:7381854/1‑93 (MQ=255)
                                                                        ccAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAat                 <  1:4042533/93‑1 (MQ=255)
                                                                        acAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAat                 <  1:7249887/92‑1 (MQ=255)
                                                                         cAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAata                >  1:4689397/1‑93 (MQ=255)
                                                                          aaTAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAatat               <  2:1255471/93‑1 (MQ=255)
                                                                            tAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGc             >  2:5471120/1‑93 (MQ=255)
                                                                            tAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGc             >  1:7139249/1‑93 (MQ=255)
                                                                             aGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATAGTGGTGGACAATATGCt            <  2:2925791/93‑1 (MQ=255)
                                                                              gTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTg           <  2:716388/93‑1 (MQ=255)
                                                                                aaTGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGGCCTGGTATGGTGGTGGACAATATGCTGaa         >  2:6547852/1‑93 (MQ=255)
                                                                                  tGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGAAAATATGCTGAata       >  1:3739775/1‑93 (MQ=255)
                                                                                  agAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAata       <  1:8208247/92‑1 (MQ=255)
                                                                                   gAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAatat      >  2:8078640/1‑93 (MQ=255)
                                                                                   gAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAatat      <  1:1201459/93‑1 (MQ=255)
                                                                                    aaCAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATAtt     >  1:1066074/1‑93 (MQ=255)
                                                                                    aaCAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATAtt     >  2:3473402/1‑93 (MQ=255)
                                                                                     aCAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTa    <  2:8003643/93‑1 (MQ=255)
                                                                                      cagcggGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTTGTATGGTGGTGGACAATATGCTGAATATTAc   >  1:8538092/1‑93 (MQ=255)
                                                                                      cagcggGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTAc   <  1:4154839/93‑1 (MQ=255)
                                                                                      cagcggGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTAc   >  1:8606890/1‑93 (MQ=255)
                                                                                       agcggGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTAcc  <  2:1065650/93‑1 (MQ=255)
                                                                                       agcggGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTAcc  <  1:1997436/93‑1 (MQ=255)
                                                                                       agcggGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTAcc  <  2:4915334/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACC  >  NC_000913/999481‑999660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACCGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGC  >  NC_000913/999478‑999664
                                                                                             |                                                                                             
TACCGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCG                                                                                               >  SRR4302540.5798749/1‑94 (MQ=60)
 ACCGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGG                                                                                              <  SRR4302540.399553/94‑1 (MQ=60)
  CCGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGG                                                                                             >  SRR4302540.7257468/1‑94 (MQ=60)
   CGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGA                                                                                            >  SRR4302540.3547429/1‑94 (MQ=60)
   CGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGA                                                                                            >  SRR4302540.50790/1‑94 (MQ=60)
   CGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGA                                                                                            >  SRR4302540.2113396/1‑94 (MQ=60)
   CGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGA                                                                                            >  SRR4302540.2113395/1‑94 (MQ=60)
    GTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAG                                                                                           <  SRR4302540.3537026/94‑1 (MQ=60)
    GTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAG                                                                                           <  SRR4302540.1740080/94‑1 (MQ=60)
     TTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGA                                                                                          <  SRR4302540.5336259/94‑1 (MQ=60)
     TTATCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGA                                                                                          <  SRR4302540.9493835/94‑1 (MQ=60)
      TCTCCAGTGTGCCATTCCTCCAGCGTCAGGGGCGAAGCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAG                                                                                         <  SRR4302540.526659/94‑1 (MQ=60)
      TCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAG                                                                                         <  SRR4302540.9130702/94‑1 (MQ=60)
        TCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCA                                                                                       >  SRR4302540.9749058/1‑94 (MQ=60)
         CCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAA                                                                                      >  SRR4302540.3401955/1‑94 (MQ=60)
         CCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAA                                                                                      <  SRR4302540.810839/94‑1 (MQ=60)
         CCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAA                                                                                      <  SRR4302540.5388206/94‑1 (MQ=60)
         CCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAA                                                                                      >  SRR4302540.5657061/1‑94 (MQ=60)
         CCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAA                                                                                      <  SRR4302540.6555999/94‑1 (MQ=60)
          CAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGTCCAAA                                                                                     >  SRR4302540.2865994/1‑94 (MQ=60)
           AGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAAT                                                                                    >  SRR4302540.4007141/1‑94 (MQ=60)
            GTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATT                                                                                   <  SRR4302540.1187755/94‑1 (MQ=60)
            GTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATT                                                                                   >  SRR4302540.5012372/1‑94 (MQ=60)
             TGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTG                                                                                  >  SRR4302540.3596741/1‑94 (MQ=60)
              GTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTG                                                                                 <  SRR4302540.3658226/94‑1 (MQ=60)
               TGCCAGTGCTCTAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGC                                                                                <  SRR4302540.5472863/94‑1 (MQ=60)
                GCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCC                                                                               >  SRR4302540.2680956/1‑94 (MQ=60)
                 CCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCC                                                                              >  SRR4302540.8202132/1‑94 (MQ=60)
                 CCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCC                                                                              >  SRR4302540.7311085/1‑94 (MQ=60)
                  CATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCA                                                                             >  SRR4302540.4919817/1‑94 (MQ=60)
                  CATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCA                                                                             <  SRR4302540.8032032/94‑1 (MQ=60)
                  CATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCA                                                                             >  SRR4302540.1164819/1‑94 (MQ=60)
                   ATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATCGTACTGAACAGCAGGAGAGCAAATTTGCCCAG                                                                            >  SRR4302540.6535816/1‑94 (MQ=60)
                   ATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAG                                                                            <  SRR4302540.4649595/94‑1 (MQ=60)
                   ATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAG                                                                            <  SRR4302540.917095/94‑1 (MQ=60)
                   ATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAG                                                                            <  SRR4302540.3788/94‑1 (MQ=60)
                   ATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAACGGGAGAGCAAATTTGCCCAG                                                                            >  SRR4302540.3700808/1‑94 (MQ=60)
                    TTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGG                                                                           >  SRR4302540.7126160/1‑94 (MQ=60)
                      ACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCC                                                                         >  SRR4302540.7792062/1‑94 (MQ=60)
                       CTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCA                                                                        <  SRR4302540.308683/94‑1 (MQ=60)
                       CTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCA                                                                        >  SRR4302540.9623190/1‑94 (MQ=60)
                        TCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCAC                                                                       <  SRR4302540.8168780/94‑1 (MQ=60)
                        TCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCAC                                                                       <  SRR4302540.9869721/94‑1 (MQ=60)
                          CAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGT                                                                     <  SRR4302540.1622363/94‑1 (MQ=60)
                             CGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGC                                                                  >  SRR4302540.3191436/1‑94 (MQ=60)
                              GTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCA                                                                 <  SRR4302540.648262/94‑1 (MQ=60)
                              GTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCA                                                                 >  SRR4302540.3538245/1‑94 (MQ=60)
                              GTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCA                                                                 <  SRR4302540.9154993/94‑1 (MQ=60)
                                CAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGT                                                               <  SRR4302540.5249047/94‑1 (MQ=60)
                                 AGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTG                                                              >  SRR4302540.5557797/1‑94 (MQ=60)
                                 AGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTG                                                              >  SRR4302540.2413537/1‑94 (MQ=60)
                                  GGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGG                                                             <  SRR4302540.2822173/94‑1 (MQ=60)
                                   GGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGG                                                            <  SRR4302540.616477/94‑1 (MQ=60)
                                     GCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGC                                                          <  SRR4302540.20607/94‑1 (MQ=60)
                                      CGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCG                                                         <  SRR4302540.5468417/94‑1 (MQ=60)
                                        AATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGA                                                       >  SRR4302540.5803905/1‑94 (MQ=60)
                                        AATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGA                                                       <  SRR4302540.2663929/94‑1 (MQ=60)
                                        AATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGA                                                       >  SRR4302540.8068698/1‑94 (MQ=60)
                                         ATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGAC                                                      >  SRR4302540.8458804/1‑94 (MQ=60)
                                         ATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGAC                                                      <  SRR4302540.525501/94‑1 (MQ=60)
                                         ATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGAC                                                      >  SRR4302540.4350705/1‑94 (MQ=60)
                                         ATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGCGCGGAC                                                      >  SRR4302540.8895178/1‑94 (MQ=60)
                                          TCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACC                                                     <  SRR4302540.931649/94‑1 (MQ=60)
                                          TCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACC                                                     <  SRR4302540.8668815/94‑1 (MQ=60)
                                          TCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACC                                                     >  SRR4302540.1135209/1‑94 (MQ=60)
                                            AAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGT                                                   <  SRR4302540.2926611/94‑1 (MQ=60)
                                            AAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGT                                                   <  SRR4302540.3665556/94‑1 (MQ=60)
                                            AAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGG                                                   >  SRR4302540.3624627/1‑94 (MQ=60)
                                            AAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGG                                                   >  SRR4302540.7935540/1‑94 (MQ=60)
                                             AATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTG                                                  <  SRR4302540.3703495/94‑1 (MQ=60)
                                                 CGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGA                                              >  SRR4302540.7357512/1‑94 (MQ=60)
                                                  GCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAA                                             <  SRR4302540.1877006/94‑1 (MQ=60)
                                                  GCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAA                                             <  SRR4302540.2199216/94‑1 (MQ=60)
                                                   CGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAAC                                            <  SRR4302540.6538641/94‑1 (MQ=60)
                                                     GTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGA                                          <  SRR4302540.1984486/94‑1 (MQ=60)
                                                       taccctgcctaaatgCAGAACCAATAATAATGAACAGGCGGATAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACC                                        <  SRR4302540.5236677/79‑1 (MQ=60)
                                                       GACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACC                                        <  SRR4302540.3416479/94‑1 (MQ=60)
                                                       GACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACC                                        <  SRR4302540.5236676/94‑1 (MQ=60)
                                                       GACACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACC                                        <  SRR4302540.4263307/94‑1 (MQ=60)
                                                         CACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTG                                      <  SRR4302540.9236543/94‑1 (MQ=60)
                                                          ACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGG                                     >  SRR4302540.2364182/1‑94 (MQ=60)
                                                          ACTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGG                                     >  SRR4302540.6370473/1‑94 (MQ=60)
                                                           CTGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGT                                    <  SRR4302540.7091533/94‑1 (MQ=60)
                                                            TGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTA                                   <  SRR4302540.6761737/94‑1 (MQ=60)
                                                            TGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTA                                   >  SRR4302540.1917210/1‑94 (MQ=60)
                                                            TGGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTA                                   >  SRR4302540.5400194/1‑94 (MQ=60)
                                                             GGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTAT                                  <  SRR4302540.4330851/94‑1 (MQ=60)
                                                             GGCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTAT                                  <  SRR4302540.1560066/94‑1 (MQ=60)
                                                              GCGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGTCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATG                                 <  SRR4302540.8361061/94‑1 (MQ=60)
                                                               CGAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGG                                >  SRR4302540.3380201/1‑94 (MQ=60)
                                                                GAAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGG                               >  SRR4302540.2980224/1‑94 (MQ=60)
                                                                 AAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTG                              <  SRR4302540.6673059/94‑1 (MQ=60)
                                                                 AAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTG                              <  SRR4302540.5865446/94‑1 (MQ=60)
                                                                 AAATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTG                              <  SRR4302540.6408451/94‑1 (MQ=60)
                                                                  AATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGG                             <  SRR4302540.1589989/94‑1 (MQ=60)
                                                                  AATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGG                             <  SRR4302540.3980970/94‑1 (MQ=60)
                                                                  AATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTCGGGCGGACCGTGGGGAACGACCTGGTATGGTGG                             <  SRR4302540.1127094/94‑1 (MQ=60)
                                                                   ATACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGT                            <  SRR4302540.5898549/94‑1 (MQ=60)
                                                                    TACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTG                           >  SRR4302540.8217993/1‑94 (MQ=60)
                                                                    TACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTG                           >  SRR4302540.1094818/1‑94 (MQ=60)
                                                                    TACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTG                           <  SRR4302540.5795072/94‑1 (MQ=60)
                                                                     ACAGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGG                          <  SRR4302540.1730221/94‑1 (MQ=60)
                                                                       AGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGAC                        <  SRR4302540.9685522/94‑1 (MQ=60)
                                                                       AGAACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGAC                        >  SRR4302540.7464109/1‑94 (MQ=60)
                                                                         AACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGCGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAA                      >  SRR4302540.4111703/1‑94 (MQ=60)
                                                                          ACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAAT                     <  SRR4302540.5829910/94‑1 (MQ=60)
                                                                          ACCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAAT                     >  SRR4302540.7566826/1‑94 (MQ=60)
                                                                           CCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACCATA                    >  SRR4302540.4448775/1‑94 (MQ=60)
                                                                           CCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATA                    >  SRR4302540.4739548/1‑94 (MQ=60)
                                                                           CCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATA                    <  SRR4302540.581313/94‑1 (MQ=60)
                                                                           CCAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATA                    <  SRR4302540.4086110/94‑1 (MQ=60)
                                                                           ACAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATA                    <  SRR4302540.7330784/94‑1 (MQ=60)
                                                                            CAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACGGTGGGGAACGACCTGGTATGGTGGTGGACAATAT                   <  SRR4302540.4093913/94‑1 (MQ=60)
                                                                            CAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATAT                   <  SRR4302540.971339/94‑1 (MQ=60)
                                                                            CAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATAT                   <  SRR4302540.1135020/94‑1 (MQ=60)
                                                                            CAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATAT                   >  SRR4302540.9049671/1‑94 (MQ=60)
                                                                            CAATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATAT                   <  SRR4302540.598949/94‑1 (MQ=60)
                                                                            CAATAGTAATGAACAGCGGGAGAGCAAATTGGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATAT                   <  SRR4302540.5121188/94‑1 (MQ=60)
                                                                            CAATAGTAATGAACAGCGGGAGAGCAAATTGGCCCAGCCCACATGGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATAT                   <  SRR4302540.7203104/94‑1 (MQ=60)
                                                                              ATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGC                 >  SRR4302540.7218784/1‑94 (MQ=60)
                                                                              ATAGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGC                 >  SRR4302540.701967/1‑94 (MQ=60)
                                                                                AGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTG               <  SRR4302540.9909246/94‑1 (MQ=60)
                                                                                AGTAATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTG               <  SRR4302540.7652426/94‑1 (MQ=60)
                                                                                   AATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGACCGACATGGTATGGTGGTGGATTATGTGCTGAAT            >  SRR4302540.9562551/1‑94 (MQ=60)
                                                                                   AATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAAT            >  SRR4302540.1186483/1‑94 (MQ=60)
                                                                                    ATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATA           >  SRR4302540.2925675/1‑94 (MQ=60)
                                                                                    ATGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGAAAATATGCTGAATA           >  SRR4302540.3780575/1‑94 (MQ=60)
                                                                                     TGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATAT          >  SRR4302540.4122073/1‑94 (MQ=60)
                                                                                     TGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATAT          <  SRR4302540.4020058/94‑1 (MQ=60)
                                                                                     AGAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATAT          <  SRR4302540.8303636/94‑1 (MQ=60)
                                                                                      GAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATT         >  SRR4302540.2153797/1‑94 (MQ=60)
                                                                                      GAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATT         >  SRR4302540.779413/1‑94 (MQ=60)
                                                                                      GAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATT         >  SRR4302540.9347238/1‑94 (MQ=60)
                                                                                      GAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATT         >  SRR4302540.1076941/1‑94 (MQ=60)
                                                                                      GAACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATT         <  SRR4302540.1213818/94‑1 (MQ=60)
                                                                                       AACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTA        >  SRR4302540.7211375/1‑94 (MQ=60)
                                                                                       AACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTA        <  SRR4302540.7332841/94‑1 (MQ=60)
                                                                                        ACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTTGTATGGTGGTGGACAATATGCTGAATATTAC       >  SRR4302540.8637305/1‑94 (MQ=60)
                                                                                        ACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTAC       >  SRR4302540.8706899/1‑94 (MQ=60)
                                                                                        ACAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTAC       <  SRR4302540.6693669/94‑1 (MQ=60)
                                                                                         CAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACC      <  SRR4302540.995942/94‑1 (MQ=60)
                                                                                         CAGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACC      <  SRR4302540.4199846/94‑1 (MQ=60)
                                                                                          AGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCG     <  SRR4302540.2019075/94‑1 (MQ=60)
                                                                                          AGCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCG     >  SRR4302540.4878375/1‑94 (MQ=60)
                                                                                           GCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGT    <  SRR4302540.380698/94‑1 (MQ=60)
                                                                                           GCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGT    <  SRR4302540.5307791/94‑1 (MQ=60)
                                                                                           GCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGT    <  SRR4302540.5819140/94‑1 (MQ=60)
                                                                                           GCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGT    <  SRR4302540.5819141/94‑1 (MQ=60)
                                                                                           GCGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGT    <  SRR4302540.6381606/94‑1 (MQ=60)
                                                                                            CGGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGGG   >  SRR4302540.2319504/1‑94 (MQ=60)
                                                                                             GGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGC  >  SRR4302540.9184471/1‑94 (MQ=60)
                                                                                             GGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGC  <  SRR4302540.4538082/94‑1 (MQ=60)
                                                                                             GGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGC  >  SRR4302540.7710122/1‑94 (MQ=60)
                                                                                             GGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGC  >  SRR4302540.6430725/1‑94 (MQ=60)
                                                                                             |                                                                                             
TACCGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGC  >  NC_000913/999478‑999664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑