Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,060,946 A→G E823G (GAG→GGG)  torA → trimethylamine N‑oxide reductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,060,9460AG100.0% 681.5 / NA 210E823G (GAG→GGG) torAtrimethylamine N‑oxide reductase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (112/98);  total (112/98)

Only 100 of 215 total aligned reads displayed.

GACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGAC  >  NC_000913/1060857‑1061035
                                                                                         |                                                                                          
gACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCa                                                                                         >  1:8880319/1‑93 (MQ=255)
gACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCa                                                                                         <  1:2683423/93‑1 (MQ=255)
 aCCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAg                                                                                        >  2:3974975/1‑93 (MQ=255)
  ccATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAgg                                                                                       <  2:4254422/93‑1 (MQ=255)
   cATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGt                                                                                      <  2:2474528/93‑1 (MQ=255)
    aTCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTg                                                                                     >  1:5852031/1‑93 (MQ=255)
     tCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGa                                                                                    >  2:5585986/1‑93 (MQ=255)
     tCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGa                                                                                    <  1:8510141/93‑1 (MQ=255)
     tCGACATCGGTACATCGCAGCTGGCACAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGa                                                                                    <  2:8653387/93‑1 (MQ=255)
        aCATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACgg                                                                                 >  2:959769/1‑93 (MQ=255)
         cATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGc                                                                                <  2:4606904/93‑1 (MQ=255)
          aTCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCg                                                                               >  1:3990919/1‑93 (MQ=255)
           tCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGt                                                                              >  2:6428231/1‑93 (MQ=255)
            cGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGtt                                                                             <  2:9740855/93‑1 (MQ=255)
             ggTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGttt                                                                            <  2:296909/93‑1 (MQ=255)
               tACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTaa                                                                          <  2:7823874/93‑1 (MQ=255)
                 cATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACg                                                                        <  2:5690170/93‑1 (MQ=255)
                   tCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAGCGGc                                                                      >  1:3475295/1‑93 (MQ=255)
                     gCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGccc                                                                    >  1:8556255/1‑93 (MQ=255)
                      cAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGcccc                                                                   <  2:5176641/93‑1 (MQ=255)
                      cAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGcccc                                                                   <  1:1111209/93‑1 (MQ=255)
                       aGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACCACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCGCCg                                                                  >  1:2518194/1‑93 (MQ=255)
                        gCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGt                                                                 >  2:4139874/1‑93 (MQ=255)
                          tGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTgg                                                               >  1:4543810/1‑93 (MQ=255)
                          tGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTgg                                                               <  1:9800746/93‑1 (MQ=255)
                          tGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTgg                                                               >  1:9704606/1‑93 (MQ=255)
                          tGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTgg                                                               <  1:8319742/93‑1 (MQ=255)
                             cgcAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGgagt                                                            >  1:7769043/1‑92 (MQ=255)
                             cgcAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGgaga                                                            <  1:451738/93‑1 (MQ=255)
                             cgcAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGgaga                                                            <  2:3131706/93‑1 (MQ=255)
                             cgcAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGgaga                                                            <  1:4874230/93‑1 (MQ=255)
                             cgcAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGgaga                                                            <  2:9509388/93‑1 (MQ=255)
                                aGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGAtgg                                                         <  2:2358700/93‑1 (MQ=255)
                                aGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGAtgg                                                         >  2:8158142/1‑93 (MQ=255)
                                 ggCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGAtggt                                                        <  1:5714628/93‑1 (MQ=255)
                                 ggCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGAtggg                                                        >  1:7096201/1‑92 (MQ=255)
                                 ggCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGAtggg                                                        >  1:2187167/1‑92 (MQ=255)
                                    gACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGgc                                                     >  2:5311943/1‑93 (MQ=255)
                                      ccAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGgcgc                                                   <  1:5223086/93‑1 (MQ=255)
                                       cAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCa                                                  <  1:6989406/93‑1 (MQ=255)
                                        aGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAg                                                 >  2:4745274/1‑93 (MQ=255)
                                        aGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAg                                                 <  1:2225142/93‑1 (MQ=255)
                                        aGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAg                                                 >  1:9363382/1‑93 (MQ=255)
                                         gTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGt                                                <  2:350909/93‑1 (MQ=255)
                                          tGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTg                                               >  1:4619132/1‑93 (MQ=255)
                                          tGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTg                                               >  2:1562101/1‑93 (MQ=255)
                                            cgcACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCg                                             >  1:9976675/1‑93 (MQ=255)
                                             gcACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑a                                           >  2:663152/1‑93 (MQ=255)
                                               acacTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGGGGCGCAGTGCG‑aaa                                         >  1:7677227/1‑92 (MQ=255)
                                                cacTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑Aata                                        >  1:9335306/1‑93 (MQ=255)
                                                cacTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑Aata                                        >  2:3416617/1‑93 (MQ=255)
                                                cacTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑Aata                                        >  2:1186513/1‑93 (MQ=255)
                                                cacTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑Aata                                        >  2:3255290/1‑93 (MQ=255)
                                                 acTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑Aatat                                       >  2:7273899/1‑93 (MQ=255)
                                                 acTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑Aatat                                       >  2:2177352/1‑93 (MQ=255)
                                                  cTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATg                                      >  1:2451774/1‑93 (MQ=255)
                                                  cTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATg                                      <  1:1578562/93‑1 (MQ=255)
                                                    aCGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGtt                                    <  2:6167137/93‑1 (MQ=255)
                                                     cGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTc                                   <  2:922382/93‑1 (MQ=255)
                                                     cGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTc                                   <  1:2636463/93‑1 (MQ=255)
                                                     cGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTc                                   <  2:741635/93‑1 (MQ=255)
                                                     cGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTc                                   >  1:4009424/1‑93 (MQ=255)
                                                      gCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTcc                                  >  1:6758798/1‑93 (MQ=255)
                                                      gCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTcc                                  <  1:4243655/93‑1 (MQ=255)
                                                      gCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTcc                                  >  2:5450921/1‑93 (MQ=255)
                                                      gCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTcc                                  <  1:2878854/93‑1 (MQ=255)
                                                      gCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTcc                                  <  1:8400322/93‑1 (MQ=255)
                                                        tgttggAACATGAGAAGTACAGCGGAACAGTGGGGCAGGTGACGGTGTTTAACGGCCCGGTGGACAGGGTGGCGCAGTGCG‑AATATGTTCCcg                                <  2:4420967/90‑1 (MQ=255)
                                                        tggtggAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCcg                                >  2:8596357/1‑93 (MQ=255)
                                                        tggtggAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCcg                                >  1:2807805/1‑93 (MQ=255)
                                                         ggtggAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCcgc                               >  1:2992448/1‑93 (MQ=255)
                                                           tggAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGt                             >  1:947727/1‑93 (MQ=255)
                                                               aaTTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCa                         >  1:3727943/1‑93 (MQ=255)
                                                                aTTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAg                        <  2:8931063/93‑1 (MQ=255)
                                                                aTTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAg                        >  2:4670780/1‑93 (MQ=255)
                                                                 ttGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAgg                       >  1:10032336/1‑93 (MQ=255)
                                                                 ttGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAgg                       <  1:5630393/93‑1 (MQ=255)
                                                                  tGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGt                      >  1:9105914/1‑93 (MQ=255)
                                                                   gagaAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTg                     <  2:9030580/93‑1 (MQ=255)
                                                                   gagaAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTg                     <  1:8010281/93‑1 (MQ=255)
                                                                   gagaAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTg                     <  2:7034266/93‑1 (MQ=255)
                                                                     gaAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTgca                   >  1:4350572/1‑91 (MQ=255)
                                                                      aaGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGaaa                  <  1:7045872/93‑1 (MQ=255)
                                                                      aaGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGaaa                  <  1:2328740/93‑1 (MQ=255)
                                                                        gTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATc                >  1:6095860/1‑93 (MQ=255)
                                                                           cAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATg             <  1:5975329/93‑1 (MQ=255)
                                                                            aaCGGACCAGTGGGGCGGGTGACGGCGTTTACCGCCCCGGTGGAAAGGGTGG‑GGAGTTCGAAATATGTT‑CCGCGTCGCAGGTGAAATCATGAc           <  2:8018697/93‑1 (MQ=255)
                                                                            aaCGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGa            <  2:4307460/93‑1 (MQ=255)
                                                                            aaCGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGa            <  2:3715086/93‑1 (MQ=255)
                                                                            aaCGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGa            >  1:351140/1‑93 (MQ=255)
                                                                            aaCGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGa            <  2:6037951/93‑1 (MQ=255)
                                                                             aCGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGAc           >  1:7840480/1‑93 (MQ=255)
                                                                              cGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGAcc          >  1:8570898/1‑93 (MQ=255)
                                                                              cGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGAcc          >  2:1436930/1‑93 (MQ=255)
                                                                                gAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGACCAc        <  2:7585943/93‑1 (MQ=255)
                                                                                gAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGACCAc        >  1:3938547/1‑93 (MQ=255)
                                                                                 aaCAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGACCACg       >  1:6481291/1‑93 (MQ=255)
                                                                                  aCAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGc      >  2:933415/1‑93 (MQ=255)
                                                                                   cAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCt     <  1:1196676/93‑1 (MQ=255)
                                                                                      tGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCCGGTGAAATCATGACCACGCTGAc  >  2:1869559/1‑93 (MQ=255)
                                                                                         |                                                                                          
GACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG‑AATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGAC  >  NC_000913/1060857‑1061035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGC  >  NC_000913/1060853‑1061038
                                                                                             |                                                                                            
TGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGG                                                                                              <  SRR4302540.3887526/94‑1 (MQ=60)
 GTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGG                                                                                             <  SRR4302540.10064632/94‑1 (MQ=60)
 GTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGG                                                                                             >  SRR4302540.4854990/1‑94 (MQ=60)
  TTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGC                                                                                            <  SRR4302540.5956413/94‑1 (MQ=60)
  TTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGC                                                                                            <  SRR4302540.2367097/94‑1 (MQ=60)
   TGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCA                                                                                           >  SRR4302540.8983090/1‑94 (MQ=60)
    GACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAG                                                                                          <  SRR4302540.2712438/94‑1 (MQ=60)
     ACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGG                                                                                         >  SRR4302540.6115559/1‑94 (MQ=60)
       CATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTG                                                                                       >  SRR4302540.5074850/1‑94 (MQ=60)
       CATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTG                                                                                       >  SRR4302540.5916754/1‑94 (MQ=60)
       CATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTG                                                                                       <  SRR4302540.4147468/94‑1 (MQ=60)
         TCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGAC                                                                                     <  SRR4302540.8609041/94‑1 (MQ=60)
           GACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGG                                                                                   >  SRR4302540.4000937/1‑94 (MQ=60)
             CATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCG                                                                                 >  SRR4302540.5369424/1‑94 (MQ=60)
             CATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCG                                                                                 >  SRR4302540.4033871/1‑94 (MQ=60)
               TCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTT                                                                               >  SRR4302540.8614121/1‑94 (MQ=60)
                   TACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAAC                                                                           <  SRR4302540.578593/94‑1 (MQ=60)
                   TACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAAC                                                                           <  SRR4302540.298865/94‑1 (MQ=60)
                   TACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAAC                                                                           <  SRR4302540.227499/94‑1 (MQ=60)
                      ATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAGCGGC                                                                        >  SRR4302540.3513137/1‑94 (MQ=60)
                      ATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGC                                                                        >  SRR4302540.4598091/1‑94 (MQ=60)
                        CGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCC                                                                      >  SRR4302540.8655639/1‑94 (MQ=60)
                         GCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCC                                                                     >  SRR4302540.2468510/1‑94 (MQ=60)
                          CAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACCACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCGCCG                                                                    >  SRR4302540.2545389/1‑94 (MQ=60)
                          CAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCG                                                                    <  SRR4302540.1122709/94‑1 (MQ=60)
                             CTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGG                                                                 >  SRR4302540.4592978/1‑94 (MQ=60)
                             CTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGG                                                                 >  SRR4302540.9818679/1‑94 (MQ=60)
                              TGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGA                                                                <  SRR4302540.9915499/94‑1 (MQ=60)
                              TGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGA                                                                <  SRR4302540.8416318/94‑1 (MQ=60)
                               GGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAG                                                               >  SRR4302540.6951968/1‑94 (MQ=60)
                                GCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGT                                                              >  SRR4302540.7859873/1‑94 (MQ=60)
                                 CGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGAT                                                             <  SRR4302540.4926535/94‑1 (MQ=60)
                                 CGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGAT                                                             <  SRR4302540.456553/94‑1 (MQ=60)
                                    AGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGG                                                          >  SRR4302540.7175170/1‑94 (MQ=60)
                                    AGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGG                                                          >  SRR4302540.2210778/1‑94 (MQ=60)
                                     GGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTG                                                         <  SRR4302540.5777531/94‑1 (MQ=60)
                                      GCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGG                                                        <  SRR4302540.4309372/94‑1 (MQ=60)
                                       CGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGC                                                       <  SRR4302540.1196693/94‑1 (MQ=60)
                                         ACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGC                                                     >  SRR4302540.2468551/1‑94 (MQ=60)
                                          CCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCA                                                    >  SRR4302540.8780977/1‑94 (MQ=60)
                                          CCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCA                                                    <  SRR4302540.5280035/94‑1 (MQ=60)
                                           CAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGGGATGGTGGCGCAG                                                   >  SRR4302540.1740083/1‑94 (MQ=60)
                                           CAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAG                                                   >  SRR4302540.9474882/1‑94 (MQ=60)
                                           CAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAG                                                   <  SRR4302540.7853601/94‑1 (MQ=60)
                                           CAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAG                                                   <  SRR4302540.7066995/94‑1 (MQ=60)
                                            AGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGT                                                  <  SRR4302540.9498024/94‑1 (MQ=60)
                                            AGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGT                                                  <  SRR4302540.2249124/94‑1 (MQ=60)
                                             GTGCGCACACTACGCTGGTGGAACTTGAGAAGGACACCGGAACAGTGGGGCAGGGGCCGGCGTTTAACGGCCCCGCGGAGTTGGTGGCGCAGTC                                                 >  SRR4302540.6845288/1‑94 (MQ=60)
                                             GTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTG                                                 >  SRR4302540.6845290/1‑94 (MQ=60)
                                             GTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTG                                                 >  SRR4302540.4668657/1‑94 (MQ=60)
                                               GCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCG                                               >  SRR4302540.10093016/1‑94 (MQ=60)
                                                CGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGA                                              >  SRR4302540.7398006/1‑94 (MQ=60)
                                                 GCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAA                                             >  SRR4302540.9161203/1‑94 (MQ=60)
                                                  CACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAAT                                            <  SRR4302540.2883167/94‑1 (MQ=60)
                                                  CACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAAT                                            <  SRR4302540.5755769/94‑1 (MQ=60)
                                                  CACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGGGGCGCAGTGCGAAA                                            >  SRR4302540.7764021/1‑94 (MQ=60)
                                                   ACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATA                                           >  SRR4302540.9446593/1‑94 (MQ=60)
                                                    CACTACGCTGGTGGAAGTTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATAT                                          <  SRR4302540.9070419/94‑1 (MQ=60)
                                                     ACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATG                                         >  SRR4302540.4524587/1‑94 (MQ=60)
                                                     ACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATG                                         <  SRR4302540.9448301/94‑1 (MQ=60)
                                                     ACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATG                                         >  SRR4302540.4695645/1‑94 (MQ=60)
                                                     ACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATG                                         >  SRR4302540.2478172/1‑94 (MQ=60)
                                                     ACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATG                                         <  SRR4302540.1325352/94‑1 (MQ=60)
                                                      CTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGT                                        <  SRR4302540.4253964/94‑1 (MQ=60)
                                                      CTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGT                                        <  SRR4302540.1595820/94‑1 (MQ=60)
                                                        ACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTC                                      <  SRR4302540.9239493/94‑1 (MQ=60)
                                                        ACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTC                                      >  SRR4302540.4052601/1‑94 (MQ=60)
                                                         CGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCC                                     >  SRR4302540.6833498/1‑94 (MQ=60)
                                                         CGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCC                                     <  SRR4302540.2555060/94‑1 (MQ=60)
                                                         CGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCC                                     <  SRR4302540.2665013/94‑1 (MQ=60)
                                                         CGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCC                                     >  SRR4302540.8543721/1‑94 (MQ=60)
                                                         CGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCC                                     <  SRR4302540.4590243/94‑1 (MQ=60)
                                                          GCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCC                                    <  SRR4302540.4289829/94‑1 (MQ=60)
                                                          GCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCC                                    <  SRR4302540.2910165/94‑1 (MQ=60)
                                                          GCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCC                                    <  SRR4302540.8497812/94‑1 (MQ=60)
                                                          GCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCC                                    <  SRR4302540.8497811/94‑1 (MQ=60)
                                                           CTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCG                                   >  SRR4302540.2838129/1‑94 (MQ=60)
                                                           CTGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCG                                   >  SRR4302540.8218267/1‑94 (MQ=60)
                                                            TGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGC                                  >  SRR4302540.3025437/1‑94 (MQ=60)
                                                            TGGTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGC                                  >  SRR4302540.3311965/1‑94 (MQ=60)
                                                              GTGGAAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGT                                >  SRR4302540.957596/1‑94 (MQ=60)
                                                                  AAATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCA                            >  SRR4302540.3768611/1‑94 (MQ=60)
                                                                   AATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAG                           >  SRR4302540.981006/1‑94 (MQ=60)
                                                                    ATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGG                          >  SRR4302540.10149032/1‑94 (MQ=60)
                                                                    ATTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGG                          >  SRR4302540.7169527/1‑94 (MQ=60)
                                                                     TTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGT                         <  SRR4302540.5692362/94‑1 (MQ=60)
                                                                     TTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGT                         >  SRR4302540.9215339/1‑94 (MQ=60)
                                                                     TTGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGG                         >  SRR4302540.7918105/1‑94 (MQ=60)
                                                                      TGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTG                        <  SRR4302540.3586680/94‑1 (MQ=60)
                                                                      TGAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTG                        >  SRR4302540.40354/1‑94 (MQ=60)
                                                                       GAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGA                       <  SRR4302540.8103469/94‑1 (MQ=60)
                                                                       GAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGA                       <  SRR4302540.3829865/94‑1 (MQ=60)
                                                                       GAGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGA                       >  SRR4302540.1029439/1‑94 (MQ=60)
                                                                        AGAAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGCA                      >  SRR4302540.4397899/1‑94 (MQ=60)
                                                                          AAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT                    <  SRR4302540.2353823/94‑1 (MQ=60)
                                                                          AAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT                    <  SRR4302540.7124221/94‑1 (MQ=60)
                                                                          AAGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT                    >  SRR4302540.8667986/1‑94 (MQ=60)
                                                                           AGTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATC                   >  SRR4302540.6163709/1‑94 (MQ=60)
                                                                            GTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCA                  >  SRR4302540.2691700/1‑94 (MQ=60)
                                                                            GTACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCA                  <  SRR4302540.3139847/94‑1 (MQ=60)
                                                                             TACAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCAT                 <  SRR4302540.6398520/94‑1 (MQ=60)
                                                                               CAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGA               >  SRR4302540.354735/1‑94 (MQ=60)
                                                                               CAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGA               >  SRR4302540.354736/1‑94 (MQ=60)
                                                                               CAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGA               <  SRR4302540.6041475/94‑1 (MQ=60)
                                                                               CAACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGA               <  SRR4302540.6041476/94‑1 (MQ=60)
                                                                               AAACGGAACAGTGGGGCAGGTGACGGGGTTTAACGGGCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGA               <  SRR4302540.2735009/94‑1 (MQ=60)
                                                                                AACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGAC              >  SRR4302540.7932088/1‑94 (MQ=60)
                                                                                AACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGAC              >  SRR4302540.8082219/1‑94 (MQ=60)
                                                                                AACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGAC              <  SRR4302540.10016938/94‑1 (MQ=60)
                                                                                 ACGGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACC             >  SRR4302540.8670470/1‑94 (MQ=60)
                                                                                   GGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCAC           >  SRR4302540.709257/1‑94 (MQ=60)
                                                                                   GGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCAC           >  SRR4302540.6690884/1‑94 (MQ=60)
                                                                                   GGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCAC           >  SRR4302540.3981100/1‑94 (MQ=60)
                                                                                   GGAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCAC           <  SRR4302540.5631349/94‑1 (MQ=60)
                                                                                    GAACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACG          >  SRR4302540.6553389/1‑94 (MQ=60)
                                                                                      ACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCT        >  SRR4302540.2496520/1‑94 (MQ=60)
                                                                                      ACAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCT        >  SRR4302540.9761365/1‑94 (MQ=60)
                                                                                       CAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTG       <  SRR4302540.224098/94‑1 (MQ=60)
                                                                                       CAGTGGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTG       <  SRR4302540.1208989/94‑1 (MQ=60)
                                                                                           GGGGCAGGTGTCGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAG   <  SRR4302540.5050582/94‑1 (MQ=60)
                                                                                           GGGGCAGGTGAGGGCGTTTAACGGCCCCGGGGAGATGGTGGCGCAAGGCGAATATGTTCCGGCGGCGCAGGAGAAATCATGACCACGCTGACCG   >  SRR4302540.5992701/1‑94 (MQ=60)
                                                                                           GGGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAG   >  SRR4302540.5992700/1‑94 (MQ=60)
                                                                                            GGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGC  <  SRR4302540.7639447/94‑1 (MQ=60)
                                                                                            GGGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGC  <  SRR4302540.7983117/94‑1 (MQ=60)
                                                                                            GGGCAGGTGACGGCGTTTAACGGACCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGC  >  SRR4302540.1353243/1‑94 (MQ=60)
                                                                                             |                                                                                            
TGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGC  >  NC_000913/1060853‑1061038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑