Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,119,088 T→C E241G (GAA→GGA)  solA ← sarcosine oxidase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,119,0880TC95.2% 778.7 / 20.2 269E241G (GAA→GGA) solAsarcosine oxidase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (7/6);  new base C (125/131);  total (132/137)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01

Only 100 of 280 total aligned reads displayed.

GAATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGC  >  NC_000913/1118998‑1119177
                                                                                          |                                                                                         
  atcgATAACCTGGCCTCCCTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCAcc                                                                                       <  2:8481644/90‑1 (MQ=255)
   tGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCg                                                                                      <  1:7289443/93‑1 (MQ=255)
   tGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCg                                                                                      >  2:5547910/1‑93 (MQ=255)
    ggATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCgg                                                                                     >  2:1438697/1‑93 (MQ=255)
    ggATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCgg                                                                                     <  1:9819344/93‑1 (MQ=255)
      aTAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTa                                                                                   >  1:4405162/1‑93 (MQ=255)
      aTAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTa                                                                                   <  1:3598431/93‑1 (MQ=255)
       tAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGaaa                                                                                  >  1:2966019/1‑93 (MQ=255)
        aaCCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTaac                                                                                 >  2:4584204/1‑92 (MQ=255)
        aaCCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTaaa                                                                                 >  2:8126249/1‑93 (MQ=255)
        aaCCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTaaa                                                                                 <  1:2166598/93‑1 (MQ=255)
         aCCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAAc                                                                                <  2:8741716/93‑1 (MQ=255)
             ggCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCg                                                                            >  1:760902/1‑93 (MQ=255)
             ggCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCg                                                                            >  1:6363262/1‑93 (MQ=255)
             ggCCTCAGTTATGTTTGCAAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCg                                                                            <  1:8946542/93‑1 (MQ=255)
              gCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCgg                                                                           <  1:4608295/93‑1 (MQ=255)
               ccTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCggg                                                                          <  1:8470364/93‑1 (MQ=255)
                 tCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGaa                                                                        >  2:6398711/1‑93 (MQ=255)
                 tCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGat                                                                        >  1:3567310/1‑92 (MQ=255)
                 tCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGaa                                                                        >  1:1076356/1‑93 (MQ=255)
                  ccGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAAt                                                                       <  2:7097467/93‑1 (MQ=255)
                   cGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAAtt                                                                      >  1:3971149/1‑93 (MQ=255)
                     ttATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTa                                                                    >  2:2963563/1‑93 (MQ=255)
                     ttATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTa                                                                    <  1:1059980/93‑1 (MQ=255)
                      tATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTAt                                                                   >  1:9497876/1‑93 (MQ=255)
                      tATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTAt                                                                   >  2:8673810/1‑93 (MQ=255)
                       aTGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTAtt                                                                  >  1:9230226/1‑93 (MQ=255)
                         gTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTAttct                                                                <  2:8570311/93‑1 (MQ=255)
                         gTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTAttct                                                                <  1:9285856/93‑1 (MQ=255)
                          tttGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTAttctt                                                               <  1:8634365/93‑1 (MQ=255)
                            tGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCa                                                             <  1:2701610/93‑1 (MQ=255)
                             gCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCAc                                                            >  1:9922827/1‑93 (MQ=255)
                             gCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCAc                                                            >  2:1771506/1‑93 (MQ=255)
                              ccAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACg                                                           >  1:5388649/1‑93 (MQ=255)
                                aaTCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCt                                                         >  1:10031362/1‑93 (MQ=255)
                                 aTCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCta                                                        >  2:6557201/1‑93 (MQ=255)
                                 aTCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCta                                                        <  2:7324227/93‑1 (MQ=255)
                                  tCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCtat                                                       >  2:9944808/1‑93 (MQ=255)
                                  tCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCtat                                                       <  1:6802623/93‑1 (MQ=255)
                                   cTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCtata                                                      >  1:6690208/1‑93 (MQ=255)
                                    ttCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAg                                                     >  2:4922236/1‑93 (MQ=255)
                                    ttCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAg                                                     >  2:7471256/1‑93 (MQ=255)
                                     tCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGc                                                    >  2:3466390/1‑93 (MQ=255)
                                        aCGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGc                                                 >  1:1838730/1‑93 (MQ=255)
                                        aCGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGc                                                 <  1:683064/93‑1 (MQ=255)
                                        aCGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGc                                                 >  2:5303220/1‑93 (MQ=255)
                                          gcgTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCa                                               >  2:905962/1‑93 (MQ=255)
                                          gcgTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCa                                               >  1:5874050/1‑93 (MQ=255)
                                           cgtcgtTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCAt                                              <  2:3594595/93‑1 (MQ=255)
                                            gtcgtTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATc                                             >  1:6731948/1‑93 (MQ=255)
                                             tcgtTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACTGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCg                                            <  2:2412875/93‑1 (MQ=255)
                                             tcgtTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCg                                            >  2:6715525/1‑93 (MQ=255)
                                              cgtTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCgg                                           <  1:3114921/93‑1 (MQ=255)
                                               gtTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGc                                          >  2:5757338/1‑93 (MQ=255)
                                                ttttCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGcc                                         <  1:7191086/93‑1 (MQ=255)
                                                ttttCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGcc                                         <  2:4491323/93‑1 (MQ=255)
                                                ttttCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGcc                                         >  2:9370218/1‑93 (MQ=255)
                                                   tCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGa                                      <  1:5264530/93‑1 (MQ=255)
                                                   tCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGa                                      <  2:4527586/93‑1 (MQ=255)
                                                   tCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGa                                      <  2:819763/93‑1 (MQ=255)
                                                    cTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGAt                                     >  2:7221297/1‑93 (MQ=255)
                                                     tGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATa                                    <  1:3410077/93‑1 (MQ=255)
                                                        cGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCa                                 <  2:60629/93‑1 (MQ=255)
                                                        cGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCa                                 <  2:6639022/93‑1 (MQ=255)
                                                         ggAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATTCCAg                                >  2:3179333/1‑93 (MQ=255)
                                                             aaCCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCaa                            <  1:2000334/93‑1 (MQ=255)
                                                                 ataataTTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATAc                        <  1:8276673/93‑1 (MQ=255)
                                                                 ataataTTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATAc                        <  1:7934549/93‑1 (MQ=255)
                                                                 ataataTTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATAc                        >  1:3850048/1‑93 (MQ=255)
                                                                  taataTTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACt                       >  1:615584/1‑93 (MQ=255)
                                                                    atatTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACttt                     >  1:3086881/1‑93 (MQ=255)
                                                                    atatTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACttt                     <  1:6496321/93‑1 (MQ=255)
                                                                     tatTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTg                    >  2:3344040/1‑93 (MQ=255)
                                                                       ttGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCg                  >  1:58125/1‑93 (MQ=255)
                                                                        tGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGt                 <  2:4347238/93‑1 (MQ=255)
                                                                         gATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTa                <  2:3119360/93‑1 (MQ=255)
                                                                         gATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGGCTGATACCAGGCAAATACTTTGCGtc                >  1:9009473/1‑92 (MQ=255)
                                                                         gATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTa                <  2:3991661/93‑1 (MQ=255)
                                                                         gATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTa                <  1:8830716/93‑1 (MQ=255)
                                                                         gATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTa                <  1:5112564/93‑1 (MQ=255)
                                                                         gATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTa                <  1:1596897/93‑1 (MQ=255)
                                                                           tCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACg              <  2:3711194/93‑1 (MQ=255)
                                                                           tCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACg              >  2:9712854/1‑93 (MQ=255)
                                                                             gCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACggg            >  2:1142278/1‑93 (MQ=255)
                                                                             gCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACggg            <  2:549198/93‑1 (MQ=255)
                                                                               cATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCt          <  1:4557423/93‑1 (MQ=255)
                                                                                aTTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTg         >  1:4892414/1‑93 (MQ=255)
                                                                                aTTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTg         >  2:4293440/1‑93 (MQ=255)
                                                                                 ttGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTgg        >  2:7487121/1‑93 (MQ=255)
                                                                                 ttGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTgg        <  1:8045930/93‑1 (MQ=255)
                                                                                 ttGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTgg        <  1:6926298/93‑1 (MQ=255)
                                                                                  tGGGCAGTCCGCCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGa       <  1:2447199/93‑1 (MQ=255)
                                                                                  tGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGa       <  1:7561046/93‑1 (MQ=255)
                                                                                  tGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGa       >  2:2002194/1‑93 (MQ=255)
                                                                                   gggCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGAc      >  2:4199315/1‑93 (MQ=255)
                                                                                    ggCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACa     <  2:4788873/93‑1 (MQ=255)
                                                                                      cAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAgg   <  1:7493799/93‑1 (MQ=255)
                                                                                       aGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGAGACCAGGCAAATACTTTGCGTACGGGCTGGACAGGc  <  2:7738136/93‑1 (MQ=255)
                                                                                       aGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGc  <  1:5653421/93‑1 (MQ=255)
                                                                                       aGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGc  <  2:2618512/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGC  >  NC_000913/1118998‑1119177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGAATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCT  >  NC_000913/1118995‑1119181
                                                                                             |                                                                                             
GCTGAATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTC                                                                                               >  SRR4302540.785598/1‑94 (MQ=60)
 CTGAATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCC                                                                                              >  SRR4302540.1115161/1‑94 (MQ=60)
  TGAATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCA                                                                                             <  SRR4302540.9645225/94‑1 (MQ=60)
  TGAATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCA                                                                                             <  SRR4302540.9645226/94‑1 (MQ=60)
   GAATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCAC                                                                                            <  SRR4302540.4966768/94‑1 (MQ=60)
    AATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACC                                                                                           <  SRR4302540.4787922/94‑1 (MQ=60)
     ATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCG                                                                                          <  SRR4302540.6012041/94‑1 (MQ=60)
     ATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCG                                                                                          >  SRR4302540.3168168/1‑94 (MQ=60)
      TGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGG                                                                                         <  SRR4302540.7370787/94‑1 (MQ=60)
       GGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGT                                                                                        <  SRR4302540.9934237/94‑1 (MQ=60)
        GATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTA                                                                                       >  SRR4302540.4452924/1‑94 (MQ=60)
        GATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTA                                                                                       >  SRR4302540.5931262/1‑94 (MQ=60)
         ATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAA                                                                                      <  SRR4302540.3637746/94‑1 (MQ=60)
         ATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGAAA                                                                                      >  SRR4302540.2998757/1‑94 (MQ=60)
          TAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAA                                                                                     >  SRR4302540.6533592/1‑94 (MQ=60)
           ACCCTGGCCTCCGTGAGGTGTGCCAATCTGCAACGCGTTGTGTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAAC                                                                                    <  SRR4302540.884650/94‑1 (MQ=60)
           AACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAAC                                                                                    <  SRR4302540.884649/94‑1 (MQ=60)
           AACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAAC                                                                                    <  SRR4302540.2189940/94‑1 (MQ=60)
            ACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACG                                                                                   <  SRR4302540.906662/94‑1 (MQ=60)
             CCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGC                                                                                  >  SRR4302540.9205287/1‑94 (MQ=60)
              CTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCC                                                                                 >  SRR4302540.2780809/1‑94 (MQ=60)
               TGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCG                                                                                >  SRR4302540.768998/1‑94 (MQ=60)
               TGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCG                                                                                >  SRR4302540.6434117/1‑94 (MQ=60)
               TGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCAGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTTAACGCCG                                                                                >  SRR4302540.768997/1‑94 (MQ=60)
                GGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGG                                                                               <  SRR4302540.8554328/94‑1 (MQ=60)
                GGCCTCAGTTATGTTTGCAAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGG                                                                               <  SRR4302540.9051801/94‑1 (MQ=60)
                 GCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGG                                                                              <  SRR4302540.4657779/94‑1 (MQ=60)
                 GCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGG                                                                              >  SRR4302540.153424/1‑94 (MQ=60)
                  CCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGA                                                                             >  SRR4302540.6844021/1‑94 (MQ=60)
                  CCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGA                                                                             <  SRR4302540.8568726/94‑1 (MQ=60)
                   CTCCGTTATGTTTGTCTATCTTCAATGCTTCGTTTTCTGCCTGAAAACCATAATATTGATCGTCATTGGGCAGTCTTCCGGTAAACGCCGGGAA                                                                            >  SRR4302540.9066217/1‑94 (MQ=53)
                   CTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAACCGCCGGGAA                                                                            >  SRR4302540.7585358/1‑94 (MQ=60)
                   CTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAT                                                                            >  SRR4302540.3606278/1‑94 (MQ=60)
                   CTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAA                                                                            >  SRR4302540.7585359/1‑94 (MQ=60)
                   CTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAA                                                                            >  SRR4302540.1087356/1‑94 (MQ=60)
                    TCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAAT                                                                           <  SRR4302540.6840570/94‑1 (MQ=60)
                     CCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATT                                                                          >  SRR4302540.4013958/1‑94 (MQ=60)
                      CGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTT                                                                         >  SRR4302540.2372934/1‑94 (MQ=60)
                      CGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTT                                                                         >  SRR4302540.904006/1‑94 (MQ=60)
                       GTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTA                                                                        >  SRR4302540.3664545/1‑94 (MQ=60)
                        TTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTAT                                                                       >  SRR4302540.9610433/1‑94 (MQ=60)
                        TTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTAT                                                                       <  SRR4302540.1070769/94‑1 (MQ=60)
                         TATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATT                                                                      >  SRR4302540.9340866/1‑94 (MQ=60)
                          ATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTC                                                                     >  SRR4302540.9296730/1‑94 (MQ=60)
                          ATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTC                                                                     >  SRR4302540.9478803/1‑94 (MQ=60)
                           TGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCT                                                                    >  SRR4302540.1747161/1‑94 (MQ=60)
                            GTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTT                                                                   <  SRR4302540.2052969/94‑1 (MQ=60)
                            GTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTT                                                                   <  SRR4302540.6438994/94‑1 (MQ=60)
                            GTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTT                                                                   <  SRR4302540.9396826/94‑1 (MQ=60)
                            GTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTT                                                                   <  SRR4302540.4521651/94‑1 (MQ=60)
                            GTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTT                                                                   >  SRR4302540.9935122/1‑94 (MQ=60)
                             TTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTC                                                                  <  SRR4302540.8734653/94‑1 (MQ=60)
                             TTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTC                                                                  >  SRR4302540.1080283/1‑94 (MQ=60)
                             TTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTC                                                                  >  SRR4302540.8573369/1‑94 (MQ=60)
                              TTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCA                                                                 <  SRR4302540.3619909/94‑1 (MQ=60)
                               TGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCAC                                                                <  SRR4302540.5576363/94‑1 (MQ=60)
                               TGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCAC                                                                >  SRR4302540.10038691/1‑94 (MQ=60)
                               TGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCAC                                                                <  SRR4302540.2730761/94‑1 (MQ=60)
                               TGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCAC                                                                >  SRR4302540.2487451/1‑94 (MQ=60)
                               TGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCAC                                                                <  SRR4302540.9438180/94‑1 (MQ=60)
                               TGCCAATCTTCAACGCGTCGTTTGCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCAC                                                                <  SRR4302540.766456/94‑1 (MQ=60)
                                GCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGTGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACG                                                               >  SRR4302540.6730983/1‑94 (MQ=60)
                                GCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACG                                                               >  SRR4302540.3430008/1‑94 (MQ=60)
                                GCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACG                                                               >  SRR4302540.5447908/1‑94 (MQ=60)
                                 CCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGC                                                              >  SRR4302540.45003/1‑94 (MQ=60)
                                 CCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGC                                                              >  SRR4302540.5419629/1‑94 (MQ=60)
                                  CAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCT                                                             >  SRR4302540.10148048/1‑94 (MQ=60)
                                    ATCTTCATCGCGTCGTTTTCTGCCGCAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTAT                                                           <  SRR4302540.5815452/94‑1 (MQ=60)
                                    ATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTAT                                                           >  SRR4302540.4637019/1‑94 (MQ=60)
                                    ATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTAT                                                           <  SRR4302540.5815450/94‑1 (MQ=60)
                                     TCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATA                                                          <  SRR4302540.6877886/94‑1 (MQ=60)
                                     TCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATA                                                          >  SRR4302540.6764073/1‑94 (MQ=60)
                                     TCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATA                                                          <  SRR4302540.6878148/94‑1 (MQ=60)
                                      CTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAG                                                         >  SRR4302540.8505393/1‑94 (MQ=60)
                                      CTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAG                                                         <  SRR4302540.306914/94‑1 (MQ=60)
                                         CAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTACCGCTATAGCGG                                                      >  SRR4302540.1286867/1‑94 (MQ=60)
                                          AACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGC                                                     >  SRR4302540.1858863/1‑94 (MQ=60)
                                           ACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCC                                                    <  SRR4302540.690394/94‑1 (MQ=60)
                                           ACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCC                                                    <  SRR4302540.690393/94‑1 (MQ=60)
                                            CGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCA                                                   >  SRR4302540.5938981/1‑94 (MQ=60)
                                            CGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCA                                                   <  SRR4302540.1872980/94‑1 (MQ=60)
                                             GCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCAT                                                  >  SRR4302540.6911115/1‑94 (MQ=60)
                                              CGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATC                                                 >  SRR4302540.6806289/1‑94 (MQ=60)
                                               GTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCG                                                <  SRR4302540.7551236/94‑1 (MQ=60)
                                                 CGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGC                                              <  SRR4302540.3148982/94‑1 (MQ=60)
                                                   TTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCT                                            <  SRR4302540.7271238/94‑1 (MQ=60)
                                                   TTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCT                                            <  SRR4302540.3097146/94‑1 (MQ=60)
                                                   TTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCT                                            <  SRR4302540.3625614/94‑1 (MQ=60)
                                                      TCTGCCGGAAACCCATAGTATTGATCGCCATTGGGATGTCCTCCGGTAAACGCCGGTAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGAT                                         <  SRR4302540.5321971/94‑1 (MQ=60)
                                                      TCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGAT                                         <  SRR4302540.2760427/94‑1 (MQ=60)
                                                      TCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGAT                                         <  SRR4302540.5321974/94‑1 (MQ=60)
                                                       CTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATA                                        <  SRR4302540.6970846/94‑1 (MQ=60)
                                                        TGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATAC                                       >  SRR4302540.9366912/1‑94 (MQ=60)
                                                        TGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATAC                                       <  SRR4302540.3447220/94‑1 (MQ=60)
                                                           CGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAG                                    <  SRR4302540.5597058/94‑1 (MQ=60)
                                                           CGGAAAACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAG                                    <  SRR4302540.3702841/94‑1 (MQ=60)
                                                            GGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGG                                   >  SRR4302540.194555/1‑94 (MQ=60)
                                                                AACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAA                               >  SRR4302540.9352103/1‑94 (MQ=60)
                                                                AACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAA                               <  SRR4302540.2021994/94‑1 (MQ=60)
                                                                AACCATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAA                               >  SRR4302540.6039138/1‑94 (MQ=60)
                                                                   CATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATAC                            >  SRR4302540.7964442/1‑94 (MQ=60)
                                                                   CATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATAC                            >  SRR4302540.7323157/1‑94 (MQ=60)
                                                                   CATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATAC                            >  SRR4302540.3891914/1‑94 (MQ=60)
                                                                    ATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACT                           <  SRR4302540.8372818/94‑1 (MQ=60)
                                                                    ATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACT                           >  SRR4302540.8163308/1‑94 (MQ=60)
                                                                    ATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACT                           <  SRR4302540.8027027/94‑1 (MQ=60)
                                                                    ATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACT                           >  SRR4302540.5442934/1‑94 (MQ=60)
                                                                    ATAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACT                           >  SRR4302540.622154/1‑94 (MQ=60)
                                                                     TAATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTT                          >  SRR4302540.981361/1‑94 (MQ=60)
                                                                      AATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTT                         <  SRR4302540.8220570/94‑1 (MQ=60)
                                                                      AATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTT                         >  SRR4302540.3120751/1‑94 (MQ=60)
                                                                      AATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTT                         <  SRR4302540.3489364/94‑1 (MQ=60)
                                                                       ATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTG                        >  SRR4302540.4645972/1‑94 (MQ=60)
                                                                       ATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTG                        <  SRR4302540.6568561/94‑1 (MQ=60)
                                                                       ATATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTG                        <  SRR4302540.3593312/94‑1 (MQ=60)
                                                                        TATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGC                       >  SRR4302540.7133446/1‑94 (MQ=60)
                                                                         ATTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCG                      >  SRR4302540.58711/1‑94 (MQ=60)
                                                                          TTGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGT                     <  SRR4302540.6360733/94‑1 (MQ=60)
                                                                           TGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGGCTGATACCAGGCAAATACTTTGCGTC                    >  SRR4302540.9117557/1‑94 (MQ=60)
                                                                           TGATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTA                    >  SRR4302540.1080945/1‑94 (MQ=60)
                                                                            TATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTAC                   <  SRR4302540.2293024/94‑1 (MQ=60)
                                                                            GATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTAC                   <  SRR4302540.1614349/94‑1 (MQ=60)
                                                                            GATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTAC                   <  SRR4302540.9993839/94‑1 (MQ=60)
                                                                            GATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTAC                   <  SRR4302540.8932846/94‑1 (MQ=60)
                                                                            GATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTAC                   <  SRR4302540.5167904/94‑1 (MQ=60)
                                                                             ATCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACG                  <  SRR4302540.6389583/94‑1 (MQ=60)
                                                                              TCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGG                 >  SRR4302540.5239493/1‑94 (MQ=60)
                                                                              TCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGG                 <  SRR4302540.6775355/94‑1 (MQ=60)
                                                                              TCGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGG                 <  SRR4302540.4433074/94‑1 (MQ=60)
                                                                               CGCCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGG                >  SRR4302540.4890653/1‑94 (MQ=60)
                                                                                 CCCTTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCGATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCT              >  SRR4302540.7966511/1‑94 (MQ=60)
                                                                                 CCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCT              >  SRR4302540.8057074/1‑94 (MQ=60)
                                                                                 CCATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCT              >  SRR4302540.8628962/1‑94 (MQ=60)
                                                                                  CATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTG             >  SRR4302540.4945022/1‑94 (MQ=60)
                                                                                  CATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTG             <  SRR4302540.4606687/94‑1 (MQ=60)
                                                                                   ATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGG            >  SRR4302540.10153756/1‑94 (MQ=60)
                                                                                   ATTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGG            >  SRR4302540.7071390/1‑94 (MQ=60)
                                                                                    TTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGA           <  SRR4302540.8139405/94‑1 (MQ=60)
                                                                                    TTGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGA           <  SRR4302540.7003384/94‑1 (MQ=60)
                                                                                     TGGGCAGTCCGCCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGAC          <  SRR4302540.2473551/94‑1 (MQ=60)
                                                                                     TGGGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGAC          <  SRR4302540.7645851/94‑1 (MQ=60)
                                                                                     TGGGCAGTCCACCCGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGAC          <  SRR4302540.2473552/94‑1 (MQ=60)
                                                                                       GGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAG        <  SRR4302540.6689631/94‑1 (MQ=60)
                                                                                       GGCAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGAGGCCATCGGCCTGATACCAGGCAACTACTTTGCGTACGGGCTGGACAG        <  SRR4302540.1803165/94‑1 (MQ=60)
                                                                                         CAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGC      <  SRR4302540.7577493/94‑1 (MQ=60)
                                                                                         CAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGC      >  SRR4302540.3899715/1‑94 (MQ=60)
                                                                                         CAGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGC      >  SRR4302540.3122625/1‑94 (MQ=60)
                                                                                          AGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCA     >  SRR4302540.7186234/1‑94 (MQ=60)
                                                                                          AGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCA     <  SRR4302540.7372394/94‑1 (MQ=60)
                                                                                          AGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCA     <  SRR4302540.43133/94‑1 (MQ=60)
                                                                                          AGTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCA     <  SRR4302540.5715664/94‑1 (MQ=60)
                                                                                           GTCCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAG    >  SRR4302540.3804578/1‑94 (MQ=60)
                                                                                             TCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCT  >  SRR4302540.8192006/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCTGAATGGATAACCTGGCCTCCGTTATGTTTGCCAATCTTCAACGCGTCGTTTTCTGCCGGAAAACCATAATATTGATCGCCATTGGGCAGTTCACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCT  >  NC_000913/1118995‑1119181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑