Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,147,973 T→C intergenic (+59/‑9) plsX → / → fabH PlsX/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,147,9730TC99.6% 848.4 / ‑5.2 261intergenic (+59/‑9)plsX/fabHPlsX/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (134/126);  total (135/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 266 total aligned reads displayed.

TGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGAC  >  NC_000913/1147883‑1148062
                                                                                          |                                                                                         
tgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGa                                                                                         <  2:7109956/93‑1 (MQ=255)
tgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGa                                                                                         <  2:689500/93‑1 (MQ=255)
tgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGa                                                                                         >  1:7101052/1‑93 (MQ=255)
   aCGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCg                                                                                      >  1:5749068/1‑93 (MQ=255)
       tGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACa                                                                                  <  2:4340761/93‑1 (MQ=255)
       gggCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACa                                                                                  <  1:481812/92‑1 (MQ=255)
        ggCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACAt                                                                                 <  2:8349339/93‑1 (MQ=255)
         gCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATg                                                                                >  1:5449408/1‑93 (MQ=255)
          cAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGt                                                                               <  1:5138283/93‑1 (MQ=255)
          cAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGt                                                                               <  2:5452606/93‑1 (MQ=255)
          cAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGt                                                                               <  1:873794/93‑1 (MQ=255)
            aaaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGtat                                                                             <  2:1150372/93‑1 (MQ=255)
            aaaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGtat                                                                             >  2:9687760/1‑93 (MQ=255)
             aaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGtata                                                                            <  1:6758144/93‑1 (MQ=255)
                 ggAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGaa                                                                        >  1:3241318/1‑93 (MQ=255)
                 ggAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGaa                                                                        <  2:3698737/93‑1 (MQ=255)
                 ggAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGaa                                                                        <  2:1312238/93‑1 (MQ=255)
                  gAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAg                                                                       >  1:4231031/1‑93 (MQ=255)
                   aaCTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGa                                                                      <  2:4712934/93‑1 (MQ=255)
                   aaCTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGa                                                                      <  1:2987442/93‑1 (MQ=255)
                    aCTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGat                                                                     >  2:4881782/1‑93 (MQ=255)
                     ctctGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGatt                                                                    >  1:2784067/1‑93 (MQ=255)
                      tctGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGatta                                                                   <  1:4033713/93‑1 (MQ=255)
                      tctGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGatta                                                                   <  2:327372/93‑1 (MQ=255)
                       ctGCGGTAGCAGGGCGCTCCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGattat                                                                  <  1:1663260/93‑1 (MQ=255)
                        tGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGattatt                                                                 <  2:6118648/93‑1 (MQ=255)
                            gTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTa                                                             <  2:5107814/93‑1 (MQ=255)
                            gTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTa                                                             <  1:9324258/93‑1 (MQ=255)
                             tAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTAc                                                            >  2:328908/1‑93 (MQ=255)
                             tAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTAc                                                            <  2:743372/93‑1 (MQ=255)
                              aGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACt                                                           >  1:9538637/1‑93 (MQ=255)
                              aGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACt                                                           >  2:3849237/1‑93 (MQ=255)
                               gCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTg                                                          >  1:626314/1‑93 (MQ=255)
                                 aGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGc                                                        <  1:8184556/93‑1 (MQ=255)
                                 aGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGc                                                        >  2:2005996/1‑93 (MQ=255)
                                  ggACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCa                                                       >  2:8881086/1‑93 (MQ=255)
                                      gCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTa                                                   <  1:9615786/93‑1 (MQ=255)
                                      gCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTa                                                   <  2:3110408/93‑1 (MQ=255)
                                      gCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTa                                                   >  2:7584850/1‑93 (MQ=255)
                                      gCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTa                                                   >  1:6076600/1‑93 (MQ=255)
                                         gCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCt                                                <  2:3013611/93‑1 (MQ=255)
                                           cAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGc                                              >  2:7575159/1‑93 (MQ=255)
                                           cAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGc                                              <  1:7163522/93‑1 (MQ=255)
                                           cAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGc                                              >  2:5547753/1‑93 (MQ=255)
                                           cAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGc                                              >  2:9451299/1‑93 (MQ=255)
                                            aGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGcc                                             <  1:3923062/93‑1 (MQ=255)
                                             gCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGccc                                            >  2:8815272/1‑93 (MQ=255)
                                             gCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGccc                                            >  1:7650579/1‑93 (MQ=255)
                                              cGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCg                                           >  1:37441/1‑93 (MQ=255)
                                                aaCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGaa                                         >  2:730906/1‑93 (MQ=255)
                                                  cTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACa                                       >  1:234871/1‑93 (MQ=255)
                                                  cTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACa                                       <  1:2638636/93‑1 (MQ=255)
                                                   tCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACaa                                      >  2:7745417/1‑93 (MQ=255)
                                                    cGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAg                                     <  2:1833852/93‑1 (MQ=255)
                                                    cGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAg                                     >  2:6519297/1‑93 (MQ=255)
                                                    cGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAg                                     >  2:9183971/1‑93 (MQ=255)
                                                    cGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAg                                     <  1:8567484/93‑1 (MQ=255)
                                                     gCAGTTTGCTAGTGACGGTGTATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAga                                    <  2:8993108/93‑2 (MQ=255)
                                                     gCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGt                                    <  2:8059550/93‑1 (MQ=255)
                                                      cAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTg                                   <  1:8361301/93‑1 (MQ=255)
                                                       aGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGc                                  <  1:4830522/93‑1 (MQ=255)
                                                       aGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGc                                  <  1:7778265/93‑1 (MQ=255)
                                                        gTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCg                                 >  1:345429/1‑93 (MQ=255)
                                                         attGCAAGGGACGGTAGATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCgg                                <  1:3155868/92‑1 (MQ=255)
                                                           tGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTGCTGGCAGCTATCTGCCCGAACAAGTGCGGAc                              >  2:445304/1‑93 (MQ=255)
                                                             cAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaa                            >  2:6661714/1‑93 (MQ=255)
                                                             cAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaa                            >  2:2760791/1‑93 (MQ=255)
                                                             cAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaa                            <  1:2788536/93‑1 (MQ=255)
                                                              aaGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaaa                           <  1:7454270/93‑1 (MQ=255)
                                                              aaGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaaa                           <  1:3354565/93‑1 (MQ=255)
                                                              aaGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaaa                           <  1:1234278/93‑1 (MQ=255)
                                                              aaGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaaa                           <  2:4549537/93‑1 (MQ=255)
                                                              aaGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCaaa                           <  1:1993249/93‑1 (MQ=255)
                                                              aaGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACaaa                           <  2:8563574/93‑1 (MQ=255)
                                                               aGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAc                          <  1:9116511/93‑1 (MQ=255)
                                                               aGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAc                          <  1:1138048/93‑1 (MQ=255)
                                                                gTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACg                         >  1:8994526/1‑93 (MQ=255)
                                                                  gACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGcc                       >  2:3956247/1‑93 (MQ=255)
                                                                  gACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGcc                       >  1:1000443/1‑93 (MQ=255)
                                                                   aCGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCg                      >  1:9191937/1‑93 (MQ=255)
                                                                    cGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGa                     <  1:5525658/93‑1 (MQ=255)
                                                                     ggTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAt                    >  1:6826747/1‑93 (MQ=255)
                                                                      gTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAANAAGTGCGGGCAAANGCCGAtt                   >  1:7061131/1‑93 (MQ=255)
                                                                      gTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAtt                   >  1:985434/1‑93 (MQ=255)
                                                                        atataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGCACAAGTGCGGACAAACGCCGATTTg                 >  2:1774296/1‑93 (MQ=255)
                                                                         tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                >  1:3594501/1‑93 (MQ=255)
                                                                         tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                >  1:4173968/1‑93 (MQ=255)
                                                                         tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                >  2:2492434/1‑93 (MQ=255)
                                                                         tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                >  1:4466340/1‑93 (MQ=255)
                                                                          ataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGa               <  1:1362110/93‑1 (MQ=255)
                                                                           taACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGaa              <  1:1889577/93‑1 (MQ=255)
                                                                           taACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGaa              >  2:8675089/1‑93 (MQ=255)
                                                                             aCCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGaaaa            >  1:4747497/1‑93 (MQ=255)
                                                                                gAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAt         <  2:7945758/93‑1 (MQ=255)
                                                                                  aaaGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAtgg       <  2:149937/93‑1 (MQ=255)
                                                                                     gTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAtggtgg    >  1:6652106/1‑93 (MQ=255)
                                                                                     gTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAtggtgg    >  1:161673/1‑93 (MQ=255)
                                                                                     gTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAtggtgg    >  2:9926742/1‑93 (MQ=255)
                                                                                      tGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGa   <  1:7237869/93‑1 (MQ=255)
                                                                                       gACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGac  >  1:1577903/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGAC  >  NC_000913/1147883‑1148062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCT  >  NC_000913/1147880‑1148066
                                                                                             |                                                                                             
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACTCTGCCAGCGAACTCGCAGTTTGCAAGTGACTGTATATAACCGAAAAGTGACC                                                                                               <  SRR4302540.8031384/94‑1 (MQ=60)
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACC                                                                                               <  SRR4302540.1128431/94‑1 (MQ=60)
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACC                                                                                               <  SRR4302540.2556888/94‑1 (MQ=60)
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACC                                                                                               <  SRR4302540.1236519/94‑1 (MQ=60)
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACC                                                                                               >  SRR4302540.7431208/1‑94 (MQ=60)
 GCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGATGCCAGCGATCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCG                                                                                              <  SRR4302540.6283114/94‑1 (MQ=60)
  CTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGA                                                                                             <  SRR4302540.9737096/94‑1 (MQ=60)
  CTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGA                                                                                             >  SRR4302540.7180064/1‑94 (MQ=60)
     GACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCG                                                                                          >  SRR4302540.5812504/1‑94 (MQ=60)
     GACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCG                                                                                          >  SRR4302540.5812503/1‑94 (MQ=60)
     GACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCG                                                                                          <  SRR4302540.4142472/94‑1 (MQ=60)
      ACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGT                                                                                         <  SRR4302540.8670209/94‑1 (MQ=60)
      ACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGT                                                                                         >  SRR4302540.2461752/1‑94 (MQ=60)
         GTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACA                                                                                      <  SRR4302540.6826191/94‑1 (MQ=60)
          TGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACAT                                                                                     <  SRR4302540.807005/94‑1 (MQ=60)
          GGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACAT                                                                                     <  SRR4302540.486973/94‑1 (MQ=60)
           GGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATG                                                                                    >  SRR4302540.5509377/1‑94 (MQ=60)
           GGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATG                                                                                    <  SRR4302540.1196237/94‑1 (MQ=60)
           GGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATG                                                                                    >  SRR4302540.9247916/1‑94 (MQ=60)
           GGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATG                                                                                    >  SRR4302540.9524071/1‑94 (MQ=60)
            GCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGT                                                                                   <  SRR4302540.9331141/94‑1 (MQ=60)
            GCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGT                                                                                   >  SRR4302540.1416410/1‑94 (MQ=60)
             CAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTA                                                                                  <  SRR4302540.3689492/94‑1 (MQ=60)
             CAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTA                                                                                  <  SRR4302540.883066/94‑1 (MQ=60)
             CAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTA                                                                                  <  SRR4302540.2943984/94‑1 (MQ=60)
             CAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTA                                                                                  <  SRR4302540.5193939/94‑1 (MQ=60)
              AAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTAT                                                                                 <  SRR4302540.5583299/94‑1 (MQ=60)
              AAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTAT                                                                                 >  SRR4302540.3572238/1‑94 (MQ=60)
                AAGCGGAACTCTGCTGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATAC                                                                               <  SRR4302540.345162/94‑1 (MQ=60)
                AAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATAC                                                                               <  SRR4302540.3042135/94‑1 (MQ=60)
                AAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATAC                                                                               <  SRR4302540.5172/94‑1 (MQ=60)
                AAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATAC                                                                               <  SRR4302540.6832832/94‑1 (MQ=60)
                 AGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACG                                                                              <  SRR4302540.7120905/94‑1 (MQ=60)
                 AGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACG                                                                              <  SRR4302540.6489675/94‑1 (MQ=60)
                  GCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGA                                                                             >  SRR4302540.9700350/1‑94 (MQ=60)
                   CGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAA                                                                            >  SRR4302540.3276593/1‑94 (MQ=60)
                    GGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAG                                                                           >  SRR4302540.8195528/1‑94 (MQ=60)
                    GGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAG                                                                           >  SRR4302540.8404025/1‑94 (MQ=60)
                    GGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAG                                                                           >  SRR4302540.4277089/1‑94 (MQ=60)
                      AACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGAT                                                                         <  SRR4302540.3020395/94‑1 (MQ=60)
                      AACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGAT                                                                         <  SRR4302540.3256487/94‑1 (MQ=60)
                       ACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGCCGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATT                                                                        >  SRR4302540.2814142/1‑94 (MQ=60)
                       ACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATT                                                                        >  SRR4302540.2814141/1‑94 (MQ=60)
                        CTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTA                                                                       >  SRR4302540.2685672/1‑94 (MQ=60)
                        CTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTA                                                                       <  SRR4302540.4673769/94‑1 (MQ=60)
                        CTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATACAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTA                                                                       >  SRR4302540.9108288/1‑94 (MQ=60)
                         TCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTAT                                                                      <  SRR4302540.522828/94‑1 (MQ=60)
                         TCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTAT                                                                      <  SRR4302540.4077210/94‑1 (MQ=60)
                          CTGCGGTAGCAGGGCGCTCCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATT                                                                     <  SRR4302540.1681372/94‑1 (MQ=60)
                          CTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATT                                                                     <  SRR4302540.7174034/94‑1 (MQ=60)
                           TGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTG                                                                    <  SRR4302540.8774505/94‑1 (MQ=60)
                            GCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGG                                                                   <  SRR4302540.1577513/94‑1 (MQ=60)
                             CGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGT                                                                  >  SRR4302540.8851338/1‑94 (MQ=60)
                             CGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGT                                                                  <  SRR4302540.5347182/94‑1 (MQ=60)
                               GTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTAC                                                                <  SRR4302540.9435484/94‑1 (MQ=60)
                               GTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTAC                                                                <  SRR4302540.1856288/94‑1 (MQ=60)
                               GTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTAC                                                                <  SRR4302540.4243418/94‑1 (MQ=60)
                                TAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACT                                                               >  SRR4302540.9651526/1‑94 (MQ=60)
                                TAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACT                                                               <  SRR4302540.5109230/94‑1 (MQ=60)
                                TAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACT                                                               <  SRR4302540.6777798/94‑1 (MQ=60)
                                 AGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTG                                                              >  SRR4302540.632980/1‑94 (MQ=60)
                                  GCAGGACGCTGCCAGCGAGCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGG                                                             <  SRR4302540.6176399/94‑1 (MQ=60)
                                    AGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCA                                                           <  SRR4302540.8279630/94‑1 (MQ=60)
                                    AGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCA                                                           <  SRR4302540.2540528/94‑1 (MQ=60)
                                    AGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCA                                                           <  SRR4302540.1977728/94‑1 (MQ=60)
                                     GGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGGAG                                                          >  SRR4302540.29461/1‑94 (MQ=60)
                                       ACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCT                                                        <  SRR4302540.6631933/94‑1 (MQ=60)
                                        CGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTA                                                       >  SRR4302540.6144172/1‑94 (MQ=60)
                                        CGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTA                                                       >  SRR4302540.6144170/1‑94 (MQ=60)
                                         GCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTAT                                                      <  SRR4302540.9729153/94‑1 (MQ=60)
                                         GCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTAT                                                      >  SRR4302540.9594249/1‑94 (MQ=60)
                                         GCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTAT                                                      >  SRR4302540.195203/1‑94 (MQ=60)
                                          CTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGATCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATC                                                     >  SRR4302540.7308048/1‑94 (MQ=60)
                                            GCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACGGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTG                                                   <  SRR4302540.127203/94‑1 (MQ=60)
                                            GCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTG                                                   <  SRR4302540.4263486/94‑1 (MQ=60)
                                            GCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTG                                                   <  SRR4302540.2270558/94‑1 (MQ=60)
                                              CAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCC                                                 <  SRR4302540.7243385/94‑1 (MQ=60)
                                              CAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCC                                                 <  SRR4302540.167909/94‑1 (MQ=60)
                                               AGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCC                                                <  SRR4302540.3965518/94‑1 (MQ=60)
                                               AGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCC                                                <  SRR4302540.3963225/94‑1 (MQ=60)
                                               AGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCC                                                >  SRR4302540.7736706/1‑94 (MQ=60)
                                               AGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCC                                                >  SRR4302540.4209159/1‑94 (MQ=60)
                                                GCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCG                                               >  SRR4302540.37828/1‑94 (MQ=60)
                                                    ACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACA                                           >  SRR4302540.7266717/1‑94 (MQ=60)
                                                    ACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACA                                           >  SRR4302540.237146/1‑94 (MQ=60)
                                                     CTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAA                                          <  SRR4302540.2667218/94‑1 (MQ=60)
                                                      TCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAG                                         <  SRR4302540.9270033/94‑1 (MQ=60)
                                                       CGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGT                                        <  SRR4302540.8666986/94‑1 (MQ=60)
                                                       CGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGG                                        >  SRR4302540.3329516/1‑94 (MQ=60)
                                                       CGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGG                                        >  SRR4302540.778071/1‑94 (MQ=60)
                                                       CGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTCGTACTGGCAGCTATGTGCCCGAACCAGT                                        >  SRR4302540.3329514/1‑94 (MQ=60)
                                                         CAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGC                                      >  SRR4302540.7818781/1‑94 (MQ=60)
                                                         CAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGC                                      <  SRR4302540.7496092/94‑1 (MQ=60)
                                                         CAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGC                                      <  SRR4302540.8458325/94‑1 (MQ=60)
                                                          aatgTGCAAGTGACGGTATATAACCGAAAAGTGACCGAACGTACATGTATACGAAGATTATTGGTACTGCCATCTATCTGCCCGAACAAGTGCG                                     <  SRR4302540.4882261/90‑1 (MQ=60)
                                                          AGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCG                                     <  SRR4302540.4882262/94‑1 (MQ=60)
                                                          AGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCG                                     >  SRR4302540.4805023/1‑94 (MQ=60)
                                                          AGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCG                                     >  SRR4302540.4394920/1‑94 (MQ=60)
                                                          AGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCG                                     <  SRR4302540.7869192/94‑1 (MQ=60)
                                                          AGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCG                                     >  SRR4302540.348940/1‑94 (MQ=60)
                                                           GTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGG                                    <  SRR4302540.6838137/94‑1 (MQ=60)
                                                            ATTGCAAGGGACGGTAGATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGG                                   <  SRR4302540.3190220/94‑1 (MQ=60)
                                                                CAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGgggggggaaa                               >  SRR4302540.2252585/1‑84 (MQ=60)
                                                                CAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAA                               <  SRR4302540.1122274/94‑1 (MQ=60)
                                                                CAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAA                               <  SRR4302540.2818650/94‑1 (MQ=60)
                                                                CAAGTGACGGTATATAACCGAAAAGGGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGCAAAAGTGCGGgcaca                               >  SRR4302540.2761465/1‑89 (MQ=60)
                                                                 AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                              <  SRR4302540.7537377/94‑1 (MQ=60)
                                                                 AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                              <  SRR4302540.1246884/94‑1 (MQ=60)
                                                                 AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                              <  SRR4302540.7537376/94‑1 (MQ=60)
                                                                 AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                              <  SRR4302540.7236114/94‑1 (MQ=60)
                                                                 AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                              <  SRR4302540.3391331/94‑1 (MQ=60)
                                                                 AAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAAC                              <  SRR4302540.2014847/94‑1 (MQ=60)
                                                                  AGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACG                             <  SRR4302540.1149961/94‑1 (MQ=60)
                                                                  AGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACG                             <  SRR4302540.9226091/94‑1 (MQ=60)
                                                                  AGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACG                             >  SRR4302540.9102421/1‑94 (MQ=60)
                                                                   GTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGC                            >  SRR4302540.4068377/1‑94 (MQ=60)
                                                                    TGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCC                           >  SRR4302540.1010603/1‑94 (MQ=60)
                                                                     GACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCG                          >  SRR4302540.9302183/1‑94 (MQ=60)
                                                                       CGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAT                        <  SRR4302540.5586507/94‑1 (MQ=60)
                                                                       CGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAT                        >  SRR4302540.5638985/1‑94 (MQ=60)
                                                                       CGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAT                        >  SRR4302540.6902334/1‑94 (MQ=60)
                                                                        GGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAANAAGTGCGGGCAAANGCCGATT                       >  SRR4302540.7139664/1‑94 (MQ=60)
                                                                        GGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATT                       >  SRR4302540.995508/1‑94 (MQ=60)
                                                                        GGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATT                       >  SRR4302540.7021625/1‑94 (MQ=60)
                                                                         GTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTT                      >  SRR4302540.3939820/1‑94 (MQ=60)
                                                                          TATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGCTTTG                     >  SRR4302540.3095724/1‑94 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACCAGTGCGGGCAAACGCCGATTTGG                    >  SRR4302540.5882995/1‑94 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                    >  SRR4302540.4219257/1‑94 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                    <  SRR4302540.1631812/94‑1 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                    <  SRR4302540.1631813/94‑1 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                    >  SRR4302540.714691/1‑94 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                    >  SRR4302540.478436/1‑94 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                    >  SRR4302540.3633771/1‑94 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                    >  SRR4302540.4514762/1‑94 (MQ=60)
                                                                             ATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAA                  <  SRR4302540.1376499/94‑1 (MQ=60)
                                                                              TAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAA                 <  SRR4302540.1910259/94‑1 (MQ=60)
                                                                               AACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAA                >  SRR4302540.4798159/1‑94 (MQ=60)
                                                                                  CGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAT             <  SRR4302540.4858525/94‑1 (MQ=60)
                                                                                   GAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATG            <  SRR4302540.1845684/94‑1 (MQ=60)
                                                                                    AAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGG           >  SRR4302540.3422365/1‑94 (MQ=60)
                                                                                    AAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGG           <  SRR4302540.9128084/94‑1 (MQ=60)
                                                                                     AAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGT          <  SRR4302540.9946465/94‑1 (MQ=60)
                                                                                      AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTG         >  SRR4302540.85748/1‑94 (MQ=60)
                                                                                      AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTG         <  SRR4302540.979763/94‑1 (MQ=60)
                                                                                      AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTG         >  SRR4302540.3766111/1‑94 (MQ=60)
                                                                                      AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTG         <  SRR4302540.1217597/94‑1 (MQ=60)
                                                                                      AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAATGCGGGCAAACGCCGATTTGGAAACAATGGTG         >  SRR4302540.85747/1‑94 (MQ=60)
                                                                                       AGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGG        >  SRR4302540.6725686/1‑94 (MQ=60)
                                                                                       AGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGG        >  SRR4302540.163298/1‑94 (MQ=60)
                                                                                         TGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGAC      >  SRR4302540.1595160/1‑94 (MQ=60)
                                                                                         TGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGAC      <  SRR4302540.7318596/94‑1 (MQ=60)
                                                                                         TGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGAC      <  SRR4302540.7318856/94‑1 (MQ=60)
                                                                                          GACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACA     >  SRR4302540.2044884/1‑94 (MQ=60)
                                                                                          GACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACA     >  SRR4302540.41816/1‑94 (MQ=60)
                                                                                          GACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACA     >  SRR4302540.41817/1‑94 (MQ=60)
                                                                                           ACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACAC    <  SRR4302540.5017794/94‑1 (MQ=60)
                                                                                             CGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCT  <  SRR4302540.6690991/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCT  >  NC_000913/1147880‑1148066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑