Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,147,973 T→C intergenic (+59/‑9) plsX → / → fabH PlsX/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,147,9730TC100.0% 667.2 / NA 203intergenic (+59/‑9)plsX/fabHPlsX/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (108/95);  total (108/95)

Only 100 of 208 total aligned reads displayed.

TGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGAC  >  NC_000913/1147883‑1148062
                                                                                          |                                                                                         
 ggACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAg                                                                                        >  1:5086209/1‑93 (MQ=255)
 ggACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAg                                                                                        >  1:3025365/1‑93 (MQ=255)
  gACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGc                                                                                       <  1:2268038/93‑1 (MQ=255)
       tGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACa                                                                                  <  2:2534709/93‑1 (MQ=255)
       tGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACa                                                                                  >  1:292752/1‑93 (MQ=255)
        ggCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACAt                                                                                 >  2:1021856/1‑93 (MQ=255)
        ggCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACAt                                                                                 <  2:4812644/93‑1 (MQ=255)
         gCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATg                                                                                >  2:5335991/1‑93 (MQ=255)
           aaaaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGta                                                                              <  2:5795856/93‑1 (MQ=255)
            anaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTNACNGTNTATAAACGAAAAGTGACCGCGCGTACCTGtat                                                                             >  2:5553985/1‑93 (MQ=255)
            aaaGCGGACCTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGtat                                                                             <  1:806730/93‑1 (MQ=255)
            aaaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGtat                                                                             >  2:3683083/1‑93 (MQ=255)
             aaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGtata                                                                            >  1:4285673/1‑93 (MQ=255)
              aGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATAc                                                                           <  1:2550047/93‑1 (MQ=255)
               gCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACg                                                                          >  2:4599271/1‑93 (MQ=255)
                 ggAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGaa                                                                        >  1:4348169/1‑93 (MQ=255)
                    aCTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGat                                                                     <  2:5586316/93‑1 (MQ=255)
                     ctctGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGatt                                                                    >  2:3310386/1‑93 (MQ=255)
                     ctctGCGGAAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGatt                                                                    >  2:6154691/1‑93 (MQ=255)
                       ctGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGattat                                                                  <  2:3907932/93‑1 (MQ=255)
                       ctGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGattat                                                                  >  1:5651979/1‑93 (MQ=255)
                        tGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGattatt                                                                 <  1:2931838/93‑1 (MQ=255)
                        tGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGattatt                                                                 <  1:289455/93‑1 (MQ=255)
                        tGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGattatt                                                                 >  1:5812496/1‑93 (MQ=255)
                         gCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTg                                                                <  1:5235917/93‑1 (MQ=255)
                          cGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTgg                                                               <  1:1414270/93‑1 (MQ=255)
                            gTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTa                                                             <  2:3078398/93‑1 (MQ=255)
                             tAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTAc                                                            <  1:4992176/93‑1 (MQ=255)
                               gCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTg                                                          >  2:2281225/1‑93 (MQ=255)
                               gCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTg                                                          <  1:4372036/93‑1 (MQ=255)
                                 aGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGc                                                        >  1:2398172/1‑93 (MQ=255)
                                  ggACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCa                                                       >  2:3090718/1‑93 (MQ=255)
                                  ggACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCa                                                       >  2:6297108/1‑93 (MQ=255)
                                  ggACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCa                                                       <  2:3756230/93‑1 (MQ=255)
                                  ggACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCa                                                       >  2:4996393/1‑93 (MQ=255)
                                      gCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTa                                                   >  2:1244121/1‑93 (MQ=255)
                                        tGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATc                                                 <  2:5192485/93‑1 (MQ=255)
                                        tGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATc                                                 >  1:4742447/1‑93 (MQ=255)
                                        tGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATc                                                 <  1:32061/93‑1 (MQ=255)
                                         gCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCt                                                <  1:6151737/93‑1 (MQ=255)
                                           cAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGc                                              >  2:573649/1‑93 (MQ=255)
                                            aGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGcc                                             >  1:3278355/1‑93 (MQ=255)
                                            aGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGcc                                             >  2:2130417/1‑93 (MQ=255)
                                            aGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGcc                                             >  1:6099510/1‑93 (MQ=255)
                                            aGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGcc                                             >  2:4454321/1‑93 (MQ=255)
                                            aGCGAACTCGCAGTTTGCAAGTGACGGAATATAACCGAAAAGTGACCGAGGGTCCAGGTATACGAAGATAATTGGTCCTGGCAGCTACCTGcc                                             >  1:3549226/1‑93 (MQ=255)
                                             gCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTCTCTGccc                                            <  2:5102531/93‑1 (MQ=255)
                                             gCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGccc                                            >  1:616694/1‑93 (MQ=255)
                                             gCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGccc                                            >  2:5447585/1‑93 (MQ=255)
                                               gAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGa                                          >  2:5165614/1‑93 (MQ=255)
                                                aaCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGaa                                         >  1:1852487/1‑93 (MQ=255)
                                                aaCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGaa                                         <  2:4711809/93‑1 (MQ=255)
                                                 aCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAAc                                        <  2:1140803/93‑1 (MQ=255)
                                                 aCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAAc                                        >  1:1467981/1‑93 (MQ=255)
                                                 aCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAAc                                        <  2:5200291/93‑1 (MQ=255)
                                                   tCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACaa                                      >  2:1949403/1‑93 (MQ=255)
                                                   tCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACaa                                      >  1:4610190/1‑93 (MQ=255)
                                                    cGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAg                                     <  2:4223105/93‑1 (MQ=255)
                                                     gCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGt                                    <  2:4669621/93‑1 (MQ=255)
                                                     gCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAAAAAGt                                    >  2:5785640/1‑93 (MQ=255)
                                                      cAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTg                                   <  1:5357185/93‑1 (MQ=255)
                                                      cAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTg                                   <  1:4535067/93‑1 (MQ=255)
                                                       aGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGc                                  >  2:2435377/1‑93 (MQ=255)
                                                       aGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGc                                  >  2:5685414/1‑93 (MQ=255)
                                                       aGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGc                                  <  2:3375201/93‑1 (MQ=255)
                                                       aGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGc                                  <  2:4078757/93‑1 (MQ=255)
                                                        gTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCg                                 <  1:4122714/93‑1 (MQ=255)
                                                        gTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCg                                 >  2:3739169/1‑93 (MQ=255)
                                                        gTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCg                                 >  2:6228109/1‑93 (MQ=255)
                                                         tttGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCgg                                >  1:1943914/1‑93 (MQ=255)
                                                         tttGCAAGTGACGGGATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAATGCgg                                >  1:6010750/1‑93 (MQ=255)
                                                            gCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCa                             >  2:2908632/1‑93 (MQ=255)
                                                                 tGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGc                        >  1:3731209/1‑93 (MQ=255)
                                                                 tGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGc                        <  2:3055320/93‑1 (MQ=255)
                                                                  gACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACgac                       >  2:1640541/1‑91 (MQ=255)
                                                                  gACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGcc                       <  2:3673195/93‑1 (MQ=255)
                                                                  gACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGcc                       <  1:5413982/93‑1 (MQ=255)
                                                                  gACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGcc                       >  1:483069/1‑93 (MQ=255)
                                                                   aCGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCg                      >  2:2483869/1‑93 (MQ=255)
                                                                    cGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGa                     >  2:5863075/1‑93 (MQ=255)
                                                                      gTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAtt                   >  2:1856302/1‑93 (MQ=255)
                                                                        atataACCGAAAAGTGACCGAGCTTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTg                 <  1:2689110/93‑1 (MQ=255)
                                                                         tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                <  2:727608/93‑1 (MQ=255)
                                                                         tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                <  1:112863/93‑1 (MQ=255)
                                                                         tataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTgg                >  1:4452281/1‑93 (MQ=255)
                                                                          ataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGa               <  2:4313641/93‑1 (MQ=255)
                                                                          ataACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGa               <  1:2633392/93‑1 (MQ=255)
                                                                            aaCCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGaaa             >  1:3156782/1‑93 (MQ=255)
                                                                             aCCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGACCAAGTGCGGGCAAACGCCGGTTTGGaaaa            >  2:1572953/1‑93 (MQ=255)
                                                                              ccGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGaaaaa           <  2:2205416/93‑1 (MQ=255)
                                                                              ccGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGaaaaa           <  2:2576872/93‑1 (MQ=255)
                                                                               cGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGaaaaaa          >  1:4089640/1‑93 (MQ=255)
                                                                               cGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGaaaaaa          >  2:2641301/1‑93 (MQ=255)
                                                                                gAAAAGGGACCGAGCGTACATGTATACGAAAGTTATTGGCACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAGAt         >  2:2257809/1‑93 (MQ=255)
                                                                                 aaaaGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAtg        >  1:2601306/1‑93 (MQ=255)
                                                                                   aaGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAAtggt      >  1:3952604/1‑93 (MQ=255)
                                                                                      tGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGa   >  1:2629009/1‑93 (MQ=255)
                                                                                      tGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGa   <  1:1059775/93‑1 (MQ=255)
                                                                                       gACCGAGCGTCAATGGATACGAAGAATATTGGGAATTGCCGGTCTCTGCCCCAAACAGTGCGGGGAAACGCCGATTTGGAAAAAATGGTGGac  >  2:4983934/1‑93 (MQ=255)
                                                                                       gACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGac  >  1:5899636/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGAC  >  NC_000913/1147883‑1148062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCT  >  NC_000913/1147880‑1148066
                                                                                             |                                                                                             
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACC                                                                                               <  SRR4302533.5974853/94‑1 (MQ=60)
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACC                                                                                               <  SRR4302533.1732196/94‑1 (MQ=60)
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACC                                                                                               <  SRR4302533.1419982/94‑1 (MQ=60)
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACC                                                                                               >  SRR4302533.2875951/1‑94 (MQ=60)
 GCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCG                                                                                              >  SRR4302533.1298948/1‑94 (MQ=60)
 GCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCG                                                                                              <  SRR4302533.5412132/94‑1 (MQ=60)
  CTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGA                                                                                             <  SRR4302533.3938195/94‑1 (MQ=60)
   TGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAG                                                                                            >  SRR4302533.5116941/1‑94 (MQ=60)
   TGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAG                                                                                            >  SRR4302533.3043875/1‑94 (MQ=60)
    GGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGC                                                                                           <  SRR4302533.4000055/94‑1 (MQ=60)
    GGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGC                                                                                           <  SRR4302533.3818598/94‑1 (MQ=60)
    GGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGC                                                                                           <  SRR4302533.2543593/94‑1 (MQ=60)
     GACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCG                                                                                          <  SRR4302533.2282068/94‑1 (MQ=60)
        GGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTAC                                                                                       <  SRR4302533.5880737/94‑1 (MQ=60)
         GTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACA                                                                                      >  SRR4302533.294539/1‑94 (MQ=60)
         GTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACA                                                                                      <  SRR4302533.1785613/94‑1 (MQ=60)
            GCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGT                                                                                   <  SRR4302533.4711953/94‑1 (MQ=60)
             CAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTA                                                                                  <  SRR4302533.300553/94‑1 (MQ=60)
             CAAAAGCGGAACTCTGCGATAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTA                                                                                  <  SRR4302533.2817074/94‑1 (MQ=60)
               AAAGCGGACCTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATA                                                                                <  SRR4302533.811469/94‑1 (MQ=60)
               AAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATA                                                                                >  SRR4302533.4311422/1‑94 (MQ=60)
                AAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATAC                                                                               <  SRR4302533.2927461/94‑1 (MQ=60)
                 AGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACG                                                                              <  SRR4302533.2565743/94‑1 (MQ=60)
                   CGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAA                                                                            >  SRR4302533.4374384/1‑94 (MQ=60)
                   CGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAA                                                                            <  SRR4302533.5248412/94‑1 (MQ=60)
                    GGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAG                                                                           >  SRR4302533.2055683/1‑94 (MQ=60)
                        CTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTA                                                                       >  SRR4302533.6199098/1‑94 (MQ=60)
                         TCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTAT                                                                      >  SRR4302533.5686139/1‑94 (MQ=60)
                          CTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATT                                                                     <  SRR4302533.5040951/94‑1 (MQ=60)
                          CTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATT                                                                     >  SRR4302533.5847602/1‑94 (MQ=60)
                          CTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATT                                                                     >  SRR4302533.5390565/1‑94 (MQ=60)
                           TGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTG                                                                    <  SRR4302533.291219/94‑1 (MQ=60)
                           TGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTG                                                                    <  SRR4302533.2949768/94‑1 (MQ=60)
                           TGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTG                                                                    >  SRR4302533.4380864/1‑94 (MQ=60)
                            GCGGTAGCAGGACTCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGG                                                                   <  SRR4302533.3622093/94‑1 (MQ=60)
                            GCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGG                                                                   <  SRR4302533.5267398/94‑1 (MQ=60)
                             CGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGT                                                                  <  SRR4302533.1422825/94‑1 (MQ=60)
                             CGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGT                                                                  <  SRR4302533.5673830/94‑1 (MQ=60)
                                TAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACT                                                               <  SRR4302533.5022441/94‑1 (MQ=60)
                                 AGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTG                                                              >  SRR4302533.4280676/1‑94 (MQ=60)
                                  GCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGG                                                             <  SRR4302533.4398427/94‑1 (MQ=60)
                                   CAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGC                                                            >  SRR4302533.2412975/1‑94 (MQ=60)
                                   CAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGC                                                            <  SRR4302533.564267/94‑1 (MQ=60)
                                     GGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAG                                                          >  SRR4302533.2948398/1‑94 (MQ=60)
                                         GCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTAT                                                      <  SRR4302533.2686890/94‑1 (MQ=60)
                                          CTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATC                                                     >  SRR4302533.4771195/1‑94 (MQ=60)
                                           TGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCT                                                    <  SRR4302533.32281/94‑1 (MQ=60)
                                            GCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTG                                                   <  SRR4302533.6188759/94‑1 (MQ=60)
                                             CCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGC                                                  >  SRR4302533.3545932/1‑94 (MQ=60)
                                              CAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCC                                                 <  SRR4302533.4534619/94‑1 (MQ=60)
                                              CAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCC                                                 >  SRR4302533.3298598/1‑94 (MQ=60)
                                              CAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCC                                                 >  SRR4302533.2647241/1‑94 (MQ=60)
                                              CAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCC                                                 >  SRR4302533.6136346/1‑94 (MQ=60)
                                              CAGCGAACTCGCAGTTTGCAAGTGACGGAATATAACCGAAAAGTGACCGAGGGTCCAGGTATACGAAGATAATTGGTCCTGGCAGCTACCTGCC                                                 >  SRR4302533.3571094/1‑94 (MQ=60)
                                               AGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGCAGATTATTGGTACTGGCAGCTATCTGCCC                                                >  SRR4302533.790325/1‑94 (MQ=60)
                                               AGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCC                                                >  SRR4302533.620313/1‑94 (MQ=60)
                                               AGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCC                                                <  SRR4302533.3097461/94‑1 (MQ=60)
                                                GCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCG                                               >  SRR4302533.6026604/1‑94 (MQ=60)
                                                  GAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAA                                             >  SRR4302533.1863782/1‑94 (MQ=60)
                                                  GAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAA                                             >  SRR4302533.2895412/1‑94 (MQ=60)
                                                   AACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAAC                                            >  SRR4302533.1476833/1‑94 (MQ=60)
                                                    ACTCGCAGATTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACA                                           <  SRR4302533.3288328/94‑1 (MQ=60)
                                                     CTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAA                                          >  SRR4302533.4638111/1‑94 (MQ=60)
                                                      TCGCAGTTTGCAAGTGACGGTATATAGCCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAG                                         <  SRR4302533.3180244/94‑1 (MQ=60)
                                                      TCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAG                                         >  SRR4302533.6069222/1‑94 (MQ=60)
                                                       CGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGT                                        >  SRR4302533.4282835/1‑94 (MQ=60)
                                                       CGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGT                                        >  SRR4302533.6185706/1‑94 (MQ=60)
                                                        GCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTG                                       >  SRR4302533.4147719/1‑94 (MQ=60)
                                                        GCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTG                                       <  SRR4302533.1885453/94‑1 (MQ=60)
                                                         CAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGC                                      <  SRR4302533.4562527/94‑1 (MQ=60)
                                                         CAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGC                                      <  SRR4302533.5389344/94‑1 (MQ=60)
                                                          AGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCG                                     >  SRR4302533.4832273/1‑94 (MQ=60)
                                                           GTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGG                                    <  SRR4302533.2305003/94‑1 (MQ=60)
                                                           GTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGG                                    <  SRR4302533.4147744/94‑1 (MQ=60)
                                                           GTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGG                                    <  SRR4302533.4429496/94‑1 (MQ=60)
                                                           GTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGG                                    >  SRR4302533.1955772/1‑94 (MQ=60)
                                                           GTTTGCAAGTGACGGGATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAATGCGG                                    >  SRR4302533.6047165/1‑94 (MQ=60)
                                                              TGCAAGTGACGGGATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCA                                 >  SRR4302533.581712/1‑94 (MQ=60)
                                                                   GTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGC                            >  SRR4302533.3754095/1‑94 (MQ=60)
                                                                    TGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCC                           >  SRR4302533.485952/1‑94 (MQ=60)
                                                                     GACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCG                          <  SRR4302533.5446538/94‑1 (MQ=60)
                                                                       CGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGAT                        >  SRR4302533.2621094/1‑94 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCTTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                    <  SRR4302533.2705595/94‑1 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                    >  SRR4302533.4479200/1‑94 (MQ=60)
                                                                           ATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGG                    >  SRR4302533.4344880/1‑94 (MQ=60)
                                                                            TATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGA                   <  SRR4302533.113578/94‑1 (MQ=60)
                                                                             ATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAA                  <  SRR4302533.2649576/94‑1 (MQ=60)
                                                                              TAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAA                 >  SRR4302533.3176194/1‑94 (MQ=60)
                                                                              TAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAA                 <  SRR4302533.1103439/94‑1 (MQ=60)
                                                                               AACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAA                >  SRR4302533.4634790/1‑94 (MQ=60)
                                                                                 CCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAA              >  SRR4302533.4114538/1‑94 (MQ=60)
                                                                                 CCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAA              >  SRR4302533.1441306/1‑94 (MQ=60)
                                                                                 CCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAA              >  SRR4302533.1238719/1‑94 (MQ=60)
                                                                                   GAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATG            <  SRR4302533.5295260/94‑1 (MQ=60)
                                                                                   GAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATG            >  SRR4302533.2617288/1‑94 (MQ=60)
                                                                                   GAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATG            >  SRR4302533.980004/1‑94 (MQ=60)
                                                                                    AAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGG           <  SRR4302533.4952870/94‑1 (MQ=60)
                                                                                     AAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGT          >  SRR4302533.3976706/1‑94 (MQ=60)
                                                                                      AAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTG         <  SRR4302533.2568710/94‑1 (MQ=60)
                                                                                        GTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGA       >  SRR4302533.2645163/1‑94 (MQ=60)
                                                                                         TGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGAC      >  SRR4302533.5935265/1‑94 (MQ=60)
                                                                                         TGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGAC      <  SRR4302533.1066006/94‑1 (MQ=60)
                                                                                           ACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACAC    >  SRR4302533.634422/1‑94 (MQ=60)
                                                                                            CCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACC   >  SRR4302533.95097/1‑94 (MQ=60)
                                                                                             CGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGGCAAACGCCGATTTGGAAAAAATGGTGGACACCT  >  SRR4302533.6187983/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCT  >  NC_000913/1147880‑1148066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑