Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,186,123	C→T	R353C (CGC→TGC)	pepT →	putative peptidase T

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,186,123	0	C	T	99.6%	1020.7 / ‑6.7	278	R353C (CGC→TGC)	pepT	putative peptidase T
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (136/141); total (137/141)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

CGCGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCCGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCAT  >  NC_000913/1186033‑1186212

cgcgAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCtgc                                                                                         <  1:7886183/93‑1 (MQ=255)

 gcgAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCtgcg                                                                                        >  1:3151346/1‑93 (MQ=255)

  cgAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCgg                                                                                       <  2:2606840/93‑1 (MQ=255)

   gagaAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCggt                                                                                      <  1:6825206/93‑1 (MQ=255)

   gagaAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCggt                                                                                      <  2:1794322/93‑1 (MQ=255)

   gagaAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCCGCggt                                                                                      >  2:9210898/1‑93 (MQ=255)

     gaAAGTGGTTGAGCATCCGCATATTCTCGATATCGCGCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCggtgg                                                                                    <  1:895560/93‑1 (MQ=255)

      aaaGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCggtggt                                                                                   <  1:5149975/93‑1 (MQ=255)

      aaaGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCggtggt                                                                                   >  1:4853346/1‑93 (MQ=255)

         gTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTAcc                                                                                >  2:9617656/1‑93 (MQ=255)

         gTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTAcc                                                                                >  1:3905446/1‑93 (MQ=255)

           ggTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGa                                                                              <  2:6577163/93‑1 (MQ=255)

           ggTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGa                                                                              <  2:5454843/93‑1 (MQ=255)

           ggTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGa                                                                              <  1:9455153/93‑1 (MQ=255)

             ttGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACg                                                                            >  2:9813734/1‑93 (MQ=255)

              tGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACgg                                                                           >  1:9940626/1‑93 (MQ=255)

              tGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACgg                                                                           >  1:2657226/1‑93 (MQ=255)

              tGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACgg                                                                           <  2:9565014/93‑1 (MQ=255)

                    tCCGCATATTCTCGATATCGCCCAGCAGGCGATGGGCGATTGCGATATTGAACCGGAACTGACACCGATCTGCGGTGGTACCGACGGCGCGCa                                                                     >  1:5300109/1‑93 (MQ=255)

                    tCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCa                                                                     >  2:9283496/1‑93 (MQ=255)

                    tCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCa                                                                     >  1:1292784/1‑93 (MQ=255)

                      cGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGt                                                                   <  2:4714722/93‑1 (MQ=255)

                       gCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGtt                                                                  >  2:2976471/1‑93 (MQ=255)

                       gCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGtt                                                                  >  1:7437859/1‑93 (MQ=255)

                       gCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGtt                                                                  <  2:1035787/93‑1 (MQ=255)

                         atatTCTTGATATCGCCCAGCGGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCTCGCAGTTGt                                                                <  2:7905160/93‑1 (MQ=255)

                          tatTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTc                                                               >  1:4713119/1‑93 (MQ=255)

                           atTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCg                                                              >  1:1840199/1‑93 (MQ=255)

                           atTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCg                                                              >  2:2214076/1‑93 (MQ=255)

                           atTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCg                                                              >  1:2559349/1‑93 (MQ=255)

                            ttCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGt                                                             >  2:9079666/1‑93 (MQ=255)

                             tctcGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGtt                                                            <  1:3489722/93‑1 (MQ=255)

                             tctcGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGtt                                                            <  2:9437495/93‑1 (MQ=255)

                             tctcGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGtt                                                            <  1:5867305/93‑1 (MQ=255)

                             tctcGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGtt                                                            <  1:4480638/93‑1 (MQ=255)

                               ccGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTa                                                          <  1:3423428/92‑1 (MQ=255)

                                 gATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATg                                                        <  2:8234662/93‑1 (MQ=255)

                                  atatCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATgg                                                       >  1:4802303/1‑93 (MQ=255)

                                   tatCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATggg                                                      >  2:6235962/1‑93 (MQ=255)

                                    atCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGa                                                     <  1:7302250/93‑1 (MQ=255)

                                      cGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGAtt                                                   <  2:1182815/93‑1 (MQ=255)

                                       gCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTa                                                  <  2:1446329/93‑1 (MQ=255)

                                       gCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGGGGTACCGACGGCGCGCAGGTGTCGTTTATGGGATTa                                                  >  2:5717418/1‑93 (MQ=255)

                                        cccAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTAc                                                 >  2:7508700/1‑93 (MQ=255)

                                        cccAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTAc                                                 >  1:9768719/1‑93 (MQ=255)

                                        cccAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGGACCGACGGCGCGCAGTTGTCGTTTATGGGATTAc                                                 >  1:5478407/1‑93 (MQ=255)

                                          cagcagGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCg                                               <  1:3017826/93‑1 (MQ=255)

                                          cagcagGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCg                                               <  1:5705846/93‑1 (MQ=255)

                                          cagcagGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCg                                               <  2:8581919/93‑1 (MQ=255)

                                            gcagGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTg                                             >  2:8167969/1‑93 (MQ=255)

                                            gcagGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTg                                             >  1:3584111/1‑93 (MQ=255)

                                            gcagGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTg                                             <  2:5283795/93‑1 (MQ=255)

                                             cagGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGc                                            >  2:8800093/1‑93 (MQ=255)

                                               ggCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGccc                                          >  1:1130684/1‑93 (MQ=255)

                                               ggCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGccc                                          >  1:2453181/1‑93 (MQ=255)

                                                    tGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAccc                                     >  2:2775711/1‑93 (MQ=255)

                                                    tGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAAcc                                     <  2:9737271/93‑1 (MQ=255)

                                                    tGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAAcc                                     >  2:4211208/1‑93 (MQ=255)

                                                    tGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAAcc                                     <  2:3078846/93‑1 (MQ=255)

                                                      cgcgATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTg                                   <  1:8603254/93‑1 (MQ=255)

                                                       gcgATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTTACGTGCCACAACCTGt                                  >  2:3650979/1‑93 (MQ=255)

                                                       gcgATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGt                                  >  2:8376399/1‑93 (MQ=255)

                                                         gATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTc                                <  2:6670851/93‑1 (MQ=255)

                                                          aTTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCa                               >  2:2651846/1‑93 (MQ=255)

                                                            tGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACt                             >  2:2465418/1‑93 (MQ=255)

                                                             gCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTg                            <  1:6084435/93‑1 (MQ=255)

                                                               gATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGc                          <  2:1901855/93‑1 (MQ=255)

                                                               gATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGc                          >  2:4384803/1‑93 (MQ=255)

                                                               gATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGc                          >  1:7598024/1‑93 (MQ=255)

                                                                atatTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCg                         >  1:3712375/1‑93 (MQ=255)

                                                                 tatTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCgg                        >  2:4137915/1‑93 (MQ=255)

                                                                 tatTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCgg                        >  1:4192260/1‑93 (MQ=255)

                                                                 tatTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCgg                        >  1:6395323/1‑93 (MQ=255)

                                                                 tatTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCgg                        <  1:7129053/93‑1 (MQ=255)

                                                                  atTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGt                       <  2:3167470/93‑1 (MQ=255)

                                                                   ttGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTTTGGGATTACCGTGCCCGGACCTGTGCACTGGAGGtt                      >  2:4831650/1‑93 (MQ=255)

                                                                   ttGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGtt                      <  2:9113577/93‑1 (MQ=255)

                                                                     gAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTAc                    <  2:1567840/93‑1 (MQ=255)

                                                                      aaCCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACa                   <  2:3385465/93‑1 (MQ=255)

                                                                       aCCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACaa                  >  1:7973245/1‑93 (MQ=255)

                                                                       aCCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACaa                  >  1:9195692/1‑93 (MQ=255)

                                                                        ccGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAAc                 <  1:8330676/93‑1 (MQ=255)

                                                                        ccGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAAc                 >  1:9075829/1‑93 (MQ=255)

                                                                        ccGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAAc                 >  2:8019658/1‑93 (MQ=255)

                                                                          ggAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTa               >  1:6947855/1‑93 (MQ=255)

                                                                          ggAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTa               <  1:1048163/93‑1 (MQ=255)

                                                                          ggAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTa               <  2:2936658/93‑1 (MQ=255)

                                                                             aCTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCa            >  1:1371837/1‑93 (MQ=255)

                                                                             aCTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCa            >  1:1008300/1‑93 (MQ=255)

                                                                               tGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATg          <  2:786373/93‑1 (MQ=255)

                                                                                gAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATgg         >  2:9768551/1‑93 (MQ=255)

                                                                                 aaaCCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGt        <  1:7676858/93‑1 (MQ=255)

                                                                                 aaaCCGATCTGCGGTGGGACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGt        <  2:7055218/93‑1 (MQ=255)

                                                                                  aaCCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCTCTGGCGGTTACAACTATCATGGTa       <  1:5771023/93‑1 (MQ=255)

                                                                                  aaCCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTa       <  2:5861726/93‑1 (MQ=255)

                                                                                   aCCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTaa      <  1:9295775/93‑1 (MQ=255)

                                                                                   aCCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTaa      >  1:384844/1‑93 (MQ=255)

                                                                                     cGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGc    >  2:4272176/1‑93 (MQ=255)

                                                                                       aTCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTCCCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCAt  <  1:2147038/93‑1 (MQ=255)

                                                                                       aTCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCAt  >  1:5818059/1‑93 (MQ=255)

CGCGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCCGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCAT  >  NC_000913/1186033‑1186212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATGCGCGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCCGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGT  >  NC_000913/1186030‑1186216

ATGCGCGAGAAGGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCT                                                                                               <  SRR4302540.8332239/94‑1 (MQ=60)

ATGCGCGAGAAGGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCT                                                                                               <  SRR4302540.3862351/94‑1 (MQ=60)

ATGCGCGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCT                                                                                               <  SRR4302540.1912651/94‑1 (MQ=60)

ATGCGCGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCT                                                                                               <  SRR4302540.9494355/94‑1 (MQ=60)

  GCGCGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCTGAACTGAAACCGATCTGC                                                                                             <  SRR4302540.5120812/94‑1 (MQ=60)

  GCGCGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGC                                                                                             <  SRR4302540.9402296/94‑1 (MQ=60)

   CGCGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCG                                                                                            <  SRR4302540.7978248/94‑1 (MQ=60)

   CGCGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCG                                                                                            >  SRR4302540.3185659/1‑94 (MQ=60)

     CGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGT                                                                                          <  SRR4302540.7085330/94‑1 (MQ=60)

      GAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTG                                                                                         <  SRR4302540.6900768/94‑1 (MQ=60)

        GAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCGCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGT                                                                                       <  SRR4302540.905076/94‑1 (MQ=60)

        GAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGT                                                                                       <  SRR4302540.3163746/94‑1 (MQ=60)

        GAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGT                                                                                       >  SRR4302540.4905366/1‑94 (MQ=60)

         AAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTA                                                                                      <  SRR4302540.7031287/94‑1 (MQ=60)

         AAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTA                                                                                      <  SRR4302540.5205813/94‑1 (MQ=60)

         AAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTA                                                                                      >  SRR4302540.6624076/1‑94 (MQ=60)

           AGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACC                                                                                    <  SRR4302540.3915927/94‑1 (MQ=60)

           AGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACC                                                                                    >  SRR4302540.3947791/1‑94 (MQ=60)

           AGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACC                                                                                    >  SRR4302540.5028845/1‑94 (MQ=60)

           AGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACC                                                                                    <  SRR4302540.310969/94‑1 (MQ=60)

            GTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCG                                                                                   >  SRR4302540.1422094/1‑94 (MQ=60)

             TGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGA                                                                                  <  SRR4302540.3541134/94‑1 (MQ=60)

              GGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGAC                                                                                 <  SRR4302540.4525539/94‑1 (MQ=60)

              GGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGAC                                                                                 <  SRR4302540.5945846/94‑1 (MQ=60)

              GGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGAC                                                                                 >  SRR4302540.3728632/1‑94 (MQ=60)

              GGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGAC                                                                                 <  SRR4302540.9567366/94‑1 (MQ=60)

                TTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGG                                                                               >  SRR4302540.10056729/1‑94 (MQ=60)

                TTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGG                                                                               >  SRR4302540.2686063/1‑94 (MQ=60)

                 TGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGC                                                                              <  SRR4302540.4723218/94‑1 (MQ=60)

                 TGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGC                                                                              <  SRR4302540.9990073/94‑1 (MQ=60)

                  GAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCG                                                                             >  SRR4302540.1171032/1‑94 (MQ=60)

                      ATCCGCATATTCTCGATATCGCCCAGCAGGCGATGGGCGATTGCGATATTGAACCGGAACTGACACCGATCTGCGGTGGTACCGACGGCGCGCA                                                                         >  SRR4302540.5358099/1‑94 (MQ=60)

                      ATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGCCGGCGCGCA                                                                         >  SRR4302540.5084140/1‑94 (MQ=60)

                      ATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCA                                                                         >  SRR4302540.2900066/1‑94 (MQ=60)

                      ATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCA                                                                         >  SRR4302540.1306153/1‑94 (MQ=60)

                      ATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCA                                                                         >  SRR4302540.1352073/1‑94 (MQ=60)

                      ATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCA                                                                         >  SRR4302540.9399283/1‑94 (MQ=60)

                      ATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCA                                                                         >  SRR4302540.5358102/1‑94 (MQ=60)

                       TCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAG                                                                        <  SRR4302540.3473066/94‑1 (MQ=60)

                       TCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAG                                                                        <  SRR4302540.7409149/94‑1 (MQ=60)

                        CCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGGGCAGT                                                                       >  SRR4302540.5661537/1‑94 (MQ=60)

                         CGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTT                                                                      >  SRR4302540.7520733/1‑94 (MQ=60)

                           CATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGT                                                                    <  SRR4302540.920066/94‑1 (MQ=60)

                            ATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTC                                                                   >  SRR4302540.5508277/1‑94 (MQ=60)

                            ATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTC                                                                   >  SRR4302540.4763479/1‑94 (MQ=60)

                            ATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTC                                                                   <  SRR4302540.4004501/94‑1 (MQ=60)

                            ATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTC                                                                   >  SRR4302540.9212511/1‑94 (MQ=60)

                            ATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTC                                                                   <  SRR4302540.2516781/94‑1 (MQ=60)

                             TATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCG                                                                  >  SRR4302540.1860358/1‑94 (MQ=60)

                             TATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCG                                                                  >  SRR4302540.7952876/1‑94 (MQ=60)

                             TATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCG                                                                  >  SRR4302540.2586937/1‑94 (MQ=60)

                               TTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTT                                                                >  SRR4302540.10083727/1‑94 (MQ=60)

                                TCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTT                                                               <  SRR4302540.3527728/94‑1 (MQ=60)

                                TCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTT                                                               <  SRR4302540.5932173/94‑1 (MQ=60)

                                TCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTT                                                               <  SRR4302540.4529228/94‑1 (MQ=60)

                                 CTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTA                                                              <  SRR4302540.3572074/94‑1 (MQ=60)

                                  TCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTAT                                                             <  SRR4302540.3430586/94‑1 (MQ=60)

                                  CCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTAT                                                             <  SRR4302540.3460649/94‑1 (MQ=60)

                                   CGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATG                                                            <  SRR4302540.269710/94‑1 (MQ=60)

                                    GATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGG                                                           >  SRR4302540.4853697/1‑94 (MQ=60)

                                      TATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGA                                                         >  SRR4302540.9387071/1‑94 (MQ=60)

                                       ATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGAT                                                        >  SRR4302540.1129251/1‑94 (MQ=60)

                                       ATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGAT                                                        <  SRR4302540.7383758/94‑1 (MQ=60)

                                        TCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATT                                                       <  SRR4302540.8167962/94‑1 (MQ=60)

                                        TCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATT                                                       <  SRR4302540.5972696/94‑1 (MQ=60)

                                          GCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTAC                                                     >  SRR4302540.8695154/1‑94 (MQ=60)

                                          GCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTAC                                                     >  SRR4302540.9883266/1‑94 (MQ=60)

                                          GCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTAC                                                     <  SRR4302540.1549930/94‑1 (MQ=60)

                                          GCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGGACCGACGGCGCGCAGTTGTCGTTTATGGGATTAC                                                     >  SRR4302540.5538713/1‑94 (MQ=60)

                                           CCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACC                                                    >  SRR4302540.7748899/1‑94 (MQ=60)

                                           CCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACC                                                    >  SRR4302540.8289076/1‑94 (MQ=60)

                                             CAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGT                                                  <  SRR4302540.5768666/94‑1 (MQ=60)

                                             CAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGT                                                  >  SRR4302540.5871463/1‑94 (MQ=60)

                                             CAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGT                                                  <  SRR4302540.3050997/94‑1 (MQ=60)

                                             CAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGT                                                  <  SRR4302540.4008456/94‑1 (MQ=60)

                                              AGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTG                                                 <  SRR4302540.8428728/94‑1 (MQ=60)

                                              AGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTG                                                 >  SRR4302540.3623263/1‑94 (MQ=60)

                                                 CGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCC                                              <  SRR4302540.6502634/94‑1 (MQ=60)

                                                 AGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCC                                              >  SRR4302540.1886809/1‑94 (MQ=60)

                                                 AGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCC                                              >  SRR4302540.1142448/1‑94 (MQ=60)

                                                 AGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCC                                              >  SRR4302540.2479587/1‑94 (MQ=60)

                                                   GCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGA                                            <  SRR4302540.9435554/94‑1 (MQ=60)

                                                    CGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAA                                           <  SRR4302540.3685399/94‑1 (MQ=60)

                                                       TGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCT                                        <  SRR4302540.9953476/94‑1 (MQ=60)

                                                       AGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTCCCGTGCCCGAACCT                                        <  SRR4302540.2670694/94‑1 (MQ=60)

                                                         CGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGT                                      <  SRR4302540.9157963/94‑1 (MQ=60)

                                                         CGCGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGT                                      <  SRR4302540.8703242/94‑1 (MQ=60)

                                                           CGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGTTGGAACCGCCTGCGCGCAGTTGTCGTTTATGGGATAACCGTGCCCGAACCTGTTC                                    >  SRR4302540.7360055/1‑94 (MQ=60)

                                                           CGATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTC                                    >  SRR4302540.7360057/1‑94 (MQ=60)

                                                            GATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCA                                   <  SRR4302540.7567745/94‑1 (MQ=60)

                                                             ATTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCAC                                  <  SRR4302540.6202176/94‑1 (MQ=60)

                                                              TTGCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTAGCGACGGCGCGCAGTTGTCGTTTATGGGATTCCCGTGCCCGAACCTGTTCACT                                 <  SRR4302540.4806591/94‑1 (MQ=60)

                                                                GCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGG                               <  SRR4302540.5886731/94‑1 (MQ=60)

                                                                GCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGG                               <  SRR4302540.6152149/94‑1 (MQ=60)

                                                                GCGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGG                               <  SRR4302540.4819245/94‑1 (MQ=60)

                                                                 CGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGC                              <  SRR4302540.7586330/94‑1 (MQ=60)

                                                                 CGATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGC                              >  SRR4302540.7683393/1‑94 (MQ=60)

                                                                  GATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCG                             <  SRR4302540.8000736/94‑1 (MQ=60)

                                                                  GATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCG                             <  SRR4302540.9693992/94‑1 (MQ=60)

                                                                  GATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCG                             >  SRR4302540.3752885/1‑94 (MQ=60)

                                                                  GATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCG                             >  SRR4302540.10057116/1‑94 (MQ=60)

                                                                  GATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCG                             <  SRR4302540.3331791/94‑1 (MQ=60)

                                                                  GATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCG                             >  SRR4302540.4891966/1‑94 (MQ=60)

                                                                   ATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGG                            >  SRR4302540.4237860/1‑94 (MQ=60)

                                                                   ATATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGG                            >  SRR4302540.6466513/1‑94 (MQ=60)

                                                                    TATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGT                           <  SRR4302540.7208462/94‑1 (MQ=60)

                                                                    TATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGT                           >  SRR4302540.3476939/1‑94 (MQ=60)

                                                                    TATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGT                           >  SRR4302540.9132513/1‑94 (MQ=60)

                                                                     ATTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTT                          <  SRR4302540.258906/94‑1 (MQ=60)

                                                                      TTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTA                         >  SRR4302540.2371340/1‑94 (MQ=60)

                                                                      TTGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTA                         >  SRR4302540.125955/1‑94 (MQ=60)

                                                                       TGAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTTC                        >  SRR4302540.5779243/1‑94 (MQ=60)

                                                                        GAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACA                       >  SRR4302540.453426/1‑94 (MQ=60)

                                                                        GAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACA                       <  SRR4302540.8862646/94‑1 (MQ=60)

                                                                        GAACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACA                       >  SRR4302540.4448489/1‑94 (MQ=60)

                                                                         AACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAA                      >  SRR4302540.8066142/1‑94 (MQ=60)

                                                                         AACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAA                      >  SRR4302540.9305997/1‑94 (MQ=60)

                                                                          ACCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAAC                     >  SRR4302540.9184832/1‑94 (MQ=60)

                                                                           CCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACT                    <  SRR4302540.2151975/94‑1 (MQ=60)

                                                                           CCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACT                    <  SRR4302540.8427373/94‑1 (MQ=60)

                                                                           CCGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACT                    <  SRR4302540.1355393/94‑1 (MQ=60)

                                                                            CGGACCTGAACCCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTA                   <  SRR4302540.3812066/94‑1 (MQ=60)

                                                                            CGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTTCAACAA                   >  SRR4302540.5581670/1‑94 (MQ=60)

                                                                            CGGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTA                   >  SRR4302540.7025175/1‑94 (MQ=60)

                                                                             GGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTTGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTtcaaaact                  >  SRR4302540.3940330/1‑86 (MQ=60)

                                                                             GGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTAT                  <  SRR4302540.1058818/94‑1 (MQ=60)

                                                                             GGAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTAT                  <  SRR4302540.1058819/94‑1 (MQ=60)

                                                                              GAACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATC                 <  SRR4302540.113317/94‑1 (MQ=60)

                                                                               AACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCA                >  SRR4302540.1386376/1‑94 (MQ=60)

                                                                               AACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCA                >  SRR4302540.1018531/1‑94 (MQ=60)

                                                                                ACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCAT               <  SRR4302540.2176229/94‑1 (MQ=60)

                                                                                ACTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCAT               >  SRR4302540.5851092/1‑94 (MQ=60)

                                                                                ACNGNANCCNATCTGCGGTGGNNCCNACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCAT               <  SRR4302540.1194705/94‑1 (MQ=60)

                                                                                 CTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATG              >  SRR4302540.7021959/1‑94 (MQ=60)

                                                                                 CTGAAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATG              >  SRR4302540.4859383/1‑94 (MQ=60)

                                                                                    AAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTA           >  SRR4302540.5324189/1‑94 (MQ=60)

                                                                                    AAACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTA           <  SRR4302540.7763642/94‑1 (MQ=60)

                                                                                     AACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCTCTGGCGGTTACAACTATCATGGTAA          <  SRR4302540.5834759/94‑1 (MQ=60)

                                                                                     AACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAA          >  SRR4302540.388820/1‑94 (MQ=60)

                                                                                     AACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAA          <  SRR4302540.5834757/94‑1 (MQ=60)

                                                                                      ACCGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAG         <  SRR4302540.9406832/94‑1 (MQ=60)

                                                                                        CGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCA       >  SRR4302540.7338179/1‑94 (MQ=60)

                                                                                        CGATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTAACGTGCCCGAACCTGTTCACTGGCGGCTACAACTAaaaagggaagca       >  SRR4302540.7508093/1‑82 (MQ=60)

                                                                                         GATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCAT      <  SRR4302540.9670408/94‑1 (MQ=60)

                                                                                         GATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCAT      >  SRR4302540.5882373/1‑94 (MQ=60)

                                                                                         GATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCAT      >  SRR4302540.5882372/1‑94 (MQ=60)

                                                                                         GATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCAT      <  SRR4302540.8830615/94‑1 (MQ=60)

                                                                                         GATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCAT      >  SRR4302540.698937/1‑94 (MQ=60)

                                                                                         GATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCAT      <  SRR4302540.7752727/94‑1 (MQ=60)

                                                                                         GATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGGAAGCAT      >  SRR4302540.8031541/1‑94 (MQ=60)

                                                                                          ATCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTCCCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATG     <  SRR4302540.2170123/94‑1 (MQ=60)

                                                                                           TCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGG    >  SRR4302540.4778803/1‑94 (MQ=60)

                                                                                           TCTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGA    <  SRR4302540.4286042/94‑1 (MQ=60)

                                                                                            CTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAG   <  SRR4302540.7252430/94‑1 (MQ=60)

                                                                                            CTGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAG   <  SRR4302540.4013098/94‑1 (MQ=60)

                                                                                             TGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGT  <  SRR4302540.8635793/94‑1 (MQ=60)

ATGCGCGAGAAAGTGGTTGAGCATCCGCATATTCTCGATATCGCCCAGCAGGCGATGCGCGATTGCGATATTGAACCGGAACTGAAACCGATCCGCGGTGGTACCGACGGCGCGCAGTTGTCGTTTATGGGATTACCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGT  >  NC_000913/1186030‑1186216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`