Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,191,403 A→G V151A (GTC→GCC)  ycfC ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,191,4030AG88.1% 512.2 / 48.0 212V151A (GTC→GCC) ycfChypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (5/20);  new base G (68/118);  total (73/139)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 225 total aligned reads displayed.

CAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCG  >  NC_000913/1191313‑1191495
                                                                                          |                                                                                            
cAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGcc                                                                                            <  1:7708612/93‑1 (MQ=255)
cAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGcc                                                                                            >  2:4011826/1‑93 (MQ=255)
   agCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGa                                                                                         <  1:5308137/93‑1 (MQ=255)
    gCAGGGGGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGaa                                                                                        <  2:3913282/93‑1 (MQ=255)
    gCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGaa                                                                                        <  1:10014852/93‑1 (MQ=255)
    gCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGaa                                                                                        <  1:8759281/93‑1 (MQ=255)
    gCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGaa                                                                                        <  1:6397465/93‑1 (MQ=255)
      aCGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATg                                                                                      <  2:881787/93‑1 (MQ=255)
      aCGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATg                                                                                      >  2:4390045/1‑93 (MQ=255)
      aCGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATg                                                                                      <  2:8211514/93‑1 (MQ=255)
        ggcggcGAGAATGCATGCCAGCAGGGTTGCGCGATCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATgcg                                                                                    <  1:1369887/93‑1 (MQ=255)
         gcggcgCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATgcgc                                                                                   >  1:9047807/1‑93 (MQ=255)
         gcggcgCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATgcgc                                                                                   <  2:8744327/93‑1 (MQ=255)
         gcggcgCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATgcgc                                                                                   <  2:5481372/93‑1 (MQ=255)
           ggcgCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCgg                                                                                 <  2:4991417/93‑1 (MQ=255)
           ggcgCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCgg                                                                                 >  2:3193426/1‑93 (MQ=255)
            gcgcGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCggg                                                                                >  2:5005053/1‑93 (MQ=255)
               cgAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCa                                                                             >  1:759299/1‑93 (MQ=255)
                gAATGCCTGCCAGCAGGGTTGCGTGAACTTTCGGCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCaa                                                                            <  1:9915067/93‑1 (MQ=255)
                gAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCaa                                                                            >  1:1208183/1‑93 (MQ=255)
                gAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCaa                                                                            <  1:2446814/93‑1 (MQ=255)
                gAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCaa                                                                            >  1:592207/1‑93 (MQ=255)
                 aaTGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAg                                                                           >  2:2702247/1‑93 (MQ=255)
                 aaTGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAg                                                                           >  1:554282/1‑93 (MQ=255)
                 aaTGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAg                                                                           <  1:3443484/93‑1 (MQ=255)
                  aTGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGc                                                                          >  1:4317000/1‑93 (MQ=255)
                  aTGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGc                                                                          <  1:9486836/93‑1 (MQ=255)
                    gcctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCgg                                                                        <  2:8156923/93‑1 (MQ=255)
                    gcctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCgg                                                                        <  2:7161660/93‑1 (MQ=255)
                     cctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCggg                                                                       <  1:1680929/93‑1 (MQ=255)
                     cctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCggg                                                                       <  2:8688280/93‑1 (MQ=255)
                     cctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGCGGCCTGAATGCGCGGGCCAAGCggg                                                                       >  1:9800575/1‑93 (MQ=255)
                      ctgacAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGc                                                                      <  2:967676/93‑1 (MQ=255)
                       tgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCt                                                                     >  2:1773235/1‑93 (MQ=255)
                         ccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTaa                                                                   <  2:5216899/93‑1 (MQ=255)
                           agcagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATc                                                                 >  1:7652035/1‑93 (MQ=255)
                           agcagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATc                                                                 <  2:8290013/93‑1 (MQ=255)
                           agcagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATc                                                                 <  2:5816110/93‑1 (MQ=255)
                            gcagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATca                                                                >  2:4712579/1‑93 (MQ=255)
                            gcagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATca                                                                <  2:7801422/93‑1 (MQ=255)
                             cagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATcac                                                               <  1:7438093/93‑1 (MQ=255)
                              agGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATcaca                                                              >  2:9570388/1‑93 (MQ=255)
                               gggTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACAt                                                             <  1:9563530/93‑1 (MQ=255)
                               gggTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACAt                                                             <  2:7763991/93‑1 (MQ=255)
                                ggTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATc                                                            >  1:6520807/1‑93 (MQ=255)
                                ggTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATc                                                            <  2:5905535/93‑1 (MQ=255)
                                ggTTGCGCAAACTTTCGCCTGCACTTGTGGACTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATc                                                            <  1:4607280/93‑1 (MQ=255)
                                 gTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCa                                                           >  2:3070560/1‑93 (MQ=255)
                                   tGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAAc                                                         <  1:9950969/93‑1 (MQ=255)
                                   tGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAAc                                                         <  2:4233339/93‑1 (MQ=255)
                                    gcgcGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACa                                                        <  1:6608712/93‑1 (MQ=255)
                                    gcgcGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACa                                                        <  1:8925681/93‑1 (MQ=255)
                                     cgcgAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACat                                                       <  2:7809794/93‑1 (MQ=255)
                                       cgAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACatag                                                     <  1:4561252/93‑1 (MQ=255)
                                          aCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACatagata                                                  >  2:6553002/1‑93 (MQ=255)
                                          aCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACatagata                                                  >  1:396808/1‑93 (MQ=255)
                                          aCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACatagata                                                  >  1:2567017/1‑93 (MQ=255)
                                            cttCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATagc                                                <  1:8623429/92‑1 (MQ=255)
                                            cttCGACTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATagc                                                <  2:3811919/92‑1 (MQ=255)
                                             ttCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATagca                                               <  1:7881321/93‑1 (MQ=255)
                                              tCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATagcag                                              <  2:9378298/93‑1 (MQ=255)
                                                gCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGcc                                            >  1:2866778/1‑93 (MQ=255)
                                                gCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGcc                                            >  2:7386990/1‑93 (MQ=255)
                                                 ccTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCa                                           <  1:8412095/93‑1 (MQ=255)
                                                  cTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCAt                                          <  1:6993381/93‑1 (MQ=255)
                                                  cTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCAt                                          <  2:1200503/93‑1 (MQ=255)
                                                   tGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATc                                         >  2:9074994/1‑93 (MQ=255)
                                                   tGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATc                                         <  2:6019994/93‑1 (MQ=255)
                                                    gCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATcg                                        >  2:6293572/1‑93 (MQ=255)
                                                      aCTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATcgcg                                      <  1:1555477/93‑1 (MQ=255)
                                                       cTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCgcgc                                     >  1:865367/1‑93 (MQ=255)
                                                          gtgGGGTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtca                                  <  1:2262464/93‑1 (MQ=255)
                                                          gtgGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtca                                  >  1:3598926/1‑93 (MQ=255)
                                                          gtgGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtca                                  <  2:6033399/93‑1 (MQ=255)
                                                          gtgGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtca                                  >  2:1480654/1‑93 (MQ=255)
                                                            gggCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtcatc                                <  2:8047149/93‑1 (MQ=255)
                                                               cTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGc                             <  1:5597050/93‑1 (MQ=255)
                                                               cTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGc                             <  1:6202551/93‑1 (MQ=255)
                                                                 ttGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGt                           <  1:3527199/93‑1 (MQ=255)
                                                                  tGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGtt                          >  1:4931474/1‑93 (MQ=255)
                                                                  tGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGtt                          <  2:2387205/93‑1 (MQ=255)
                                                                   gCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGttt                         >  1:482648/1‑93 (MQ=255)
                                                                    cAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTc                        <  2:7821967/93‑1 (MQ=255)
                                                                    cAGGACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTc                        <  2:7523143/93‑1 (MQ=255)
                                                                     aGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCg                       >  2:5521035/1‑93 (MQ=255)
                                                                      gTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCgg                      <  2:6557620/93‑1 (MQ=255)
                                                                       tACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGa                     >  2:1021510/1‑93 (MQ=255)
                                                                        aCAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGAc                    <  2:6236353/93‑1 (MQ=255)
                                                                        aCAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGAc                    >  2:3384848/1‑93 (MQ=255)
                                                                          agcagGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTg                  >  1:3678956/1‑93 (MQ=255)
                                                                           gcagGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGt                 <  2:6347461/93‑1 (MQ=255)
                                                                            tgggggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTa                <  2:1740302/91‑1 (MQ=255)
                                                                             agGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaa               >  2:2949156/1‑93 (MQ=255)
                                                                             agGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaa               <  2:7581799/93‑1 (MQ=255)
                                                                              ggggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaaa              <  1:6435392/93‑1 (MQ=255)
                                                                                 gAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCg           <  2:24834/93‑1 (MQ=255)
                                                                                  aaCCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATATCAGCCATCGCTCTCATCAGCGTTTCGGACTGTAAATCGa          <  2:1979599/93‑1 (MQ=255)
                                                                                    ccGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAg        <  1:9029920/93‑1 (MQ=255)
                                                                                      ggTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAgtg      >  1:3283204/1‑93 (MQ=255)
                                                                                      ggTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAgtg      <  2:8630113/93‑1 (MQ=255)
                                                                                          |                                                                                            
CAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCG  >  NC_000913/1191313‑1191495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCG  >  NC_000913/1191310‑1191495
                                                                                             |                                                                                            
TGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGG                                                                                              <  SRR4302540.3504879/94‑1 (MQ=60)
TGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGG                                                                                              <  SRR4302540.8477005/94‑1 (MQ=60)
 GCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGC                                                                                             <  SRR4302540.4298615/94‑1 (MQ=60)
 GCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGC                                                                                             <  SRR4302540.9803858/94‑1 (MQ=60)
 GCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGC                                                                                             <  SRR4302540.7534691/94‑1 (MQ=60)
   CAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCT                                                                                           >  SRR4302540.6622852/1‑94 (MQ=60)
   CAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCT                                                                                           <  SRR4302540.7797089/94‑1 (MQ=60)
      AGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAGCCGGTGGCCTGAA                                                                                        >  SRR4302540.8928794/1‑94 (MQ=60)
      AGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAA                                                                                        <  SRR4302540.5366232/94‑1 (MQ=60)
       GCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAAT                                                                                       <  SRR4302540.8860857/94‑1 (MQ=60)
       GCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAAT                                                                                       <  SRR4302540.10131437/94‑1 (MQ=60)
       GCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAAT                                                                                       <  SRR4302540.6468687/94‑1 (MQ=60)
       GCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAAT                                                                                       <  SRR4302540.6468688/94‑1 (MQ=60)
           GGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGG                                                                                   >  SRR4302540.10043760/1‑94 (MQ=60)
           GGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGC                                                                                   >  SRR4302540.9156423/1‑94 (MQ=60)
           GGCGGCGAGAATGCATGCCAGCAGGGTTGCGCGATCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGC                                                                                   <  SRR4302540.1384404/94‑1 (MQ=60)
            GCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCG                                                                                  <  SRR4302540.1543466/94‑1 (MQ=60)
             GGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGG                                                                                 <  SRR4302540.6558035/94‑1 (MQ=60)
             CGGCGCTAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGG                                                                                 <  SRR4302540.4970685/94‑1 (MQ=60)
             CGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGG                                                                                 <  SRR4302540.6163474/94‑1 (MQ=60)
               GCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGC                                                                               >  SRR4302540.8172773/1‑94 (MQ=60)
                CGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCC                                                                              >  SRR4302540.7780333/1‑94 (MQ=60)
                CGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCC                                                                              >  SRR4302540.10092864/1‑94 (MQ=60)
                CGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCC                                                                              <  SRR4302540.6650045/94‑1 (MQ=60)
                 GCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCA                                                                             >  SRR4302540.767380/1‑94 (MQ=60)
                  CGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAA                                                                            >  SRR4302540.598580/1‑94 (MQ=60)
                  CGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAA                                                                            >  SRR4302540.1220581/1‑94 (MQ=60)
                  CGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAA                                                                            >  SRR4302540.4712253/1‑94 (MQ=60)
                  CGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAA                                                                            >  SRR4302540.434588/1‑94 (MQ=60)
                   GAATGCCTGCCAGCAGGGTTGCGTGAACTTTCGGCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAG                                                                           <  SRR4302540.10030870/94‑1 (MQ=60)
                   GAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAG                                                                           <  SRR4302540.2473161/94‑1 (MQ=60)
                   GAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAG                                                                           >  SRR4302540.560326/1‑94 (MQ=60)
                    AATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGC                                                                          <  SRR4302540.3480930/94‑1 (MQ=60)
                    AATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGC                                                                          >  SRR4302540.4363999/1‑94 (MQ=60)
                     ATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCG                                                                         <  SRR4302540.9599312/94‑1 (MQ=60)
                     ATGCCTGCCAGCAGGGTTGCGCGAACTGTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCG                                                                         <  SRR4302540.789144/94‑1 (MQ=60)
                       GCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGCGGCCTGAATGCGCGGGCCAAGCGGG                                                                       >  SRR4302540.9915326/1‑94 (MQ=60)
                        CCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGC                                                                      <  SRR4302540.1699175/94‑1 (MQ=60)
                         CTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCT                                                                     <  SRR4302540.8846466/94‑1 (MQ=60)
                             CAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATC                                                                 >  SRR4302540.4412745/1‑94 (MQ=60)
                             CAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATC                                                                 >  SRR4302540.7738176/1‑94 (MQ=60)
                              AGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTTGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCA                                                                <  SRR4302540.2556758/94‑1 (MQ=60)
                                CAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACA                                                              <  SRR4302540.7520973/94‑1 (MQ=60)
                                 AGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACAT                                                             >  SRR4302540.2848785/1‑94 (MQ=60)
                                  GGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATC                                                            <  SRR4302540.9676575/94‑1 (MQ=60)
                                  GGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATC                                                            <  SRR4302540.1576272/94‑1 (MQ=60)
                                  GGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATC                                                            >  SRR4302540.6593236/1‑94 (MQ=60)
                                   GGTTGCGCAAACTTTCGCCTGCACTTGTGGACTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCA                                                           <  SRR4302540.4656761/94‑1 (MQ=60)
                                    GTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAA                                                          >  SRR4302540.5619696/1‑94 (MQ=60)
                                      TGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACA                                                        <  SRR4302540.10067142/94‑1 (MQ=60)
                                       GCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACAT                                                       <  SRR4302540.9029471/94‑1 (MQ=60)
                                       GCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACAT                                                       <  SRR4302540.6681886/94‑1 (MQ=60)
                                          CGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGA                                                    <  SRR4302540.4610534/94‑1 (MQ=60)
                                           GAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGAT                                                   <  SRR4302540.355844/94‑1 (MQ=60)
                                           GAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGAT                                                   <  SRR4302540.2823435/94‑1 (MQ=60)
                                            AACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATA                                                  >  SRR4302540.2594693/1‑94 (MQ=60)
                                            AACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATA                                                  <  SRR4302540.8683719/94‑1 (MQ=60)
                                            AACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATA                                                  <  SRR4302540.4628362/94‑1 (MQ=60)
                                            AACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATA                                                  >  SRR4302540.400922/1‑94 (MQ=60)
                                             ACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAG                                                 <  SRR4302540.5377870/94‑1 (MQ=60)
                                               TTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCA                                               <  SRR4302540.6246134/94‑1 (MQ=60)
                                               TTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCA                                               <  SRR4302540.9149983/94‑1 (MQ=60)
                                               CTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCA                                               <  SRR4302540.8723601/94‑1 (MQ=60)
                                                TTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAG                                              <  SRR4302540.7973352/94‑1 (MQ=60)
                                                TTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAG                                              <  SRR4302540.8744102/94‑1 (MQ=60)
                                                  CGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCC                                            >  SRR4302540.2897893/1‑94 (MQ=60)
                                                    CCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCAT                                          <  SRR4302540.8509735/94‑1 (MQ=60)
                                                     CTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATC                                         <  SRR4302540.7071001/94‑1 (MQ=60)
                                                      TGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCG                                        <  SRR4302540.7567013/94‑1 (MQ=60)
                                                         ACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGC                                     <  SRR4302540.1572582/94‑1 (MQ=60)
                                                         ACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGC                                     >  SRR4302540.874554/1‑94 (MQ=60)
                                                         ACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGC                                     <  SRR4302540.5146316/94‑1 (MQ=60)
                                                          CTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCC                                    >  SRR4302540.7689514/1‑94 (MQ=60)
                                                           TTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTC                                   >  SRR4302540.9055181/1‑94 (MQ=60)
                                                           TTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCAATCGCGCTC                                   >  SRR4302540.3205172/1‑94 (MQ=60)
                                                            TGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCA                                  <  SRR4302540.10012328/94‑1 (MQ=60)
                                                            TGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCA                                  >  SRR4302540.3638250/1‑94 (MQ=60)
                                                            TGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCA                                  >  SRR4302540.3638249/1‑94 (MQ=60)
                                                             TTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCAT                                 <  SRR4302540.2286742/94‑1 (MQ=60)
                                                             GTGGGGTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCAT                                 <  SRR4302540.2286744/94‑1 (MQ=60)
                                                             GTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCAT                                 <  SRR4302540.6830672/94‑1 (MQ=60)
                                                             GTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCAT                                 >  SRR4302540.246655/1‑94 (MQ=60)
                                                              TGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATC                                <  SRR4302540.9742064/94‑1 (MQ=60)
                                                               GGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCA                               <  SRR4302540.981385/94‑1 (MQ=60)
                                                                GGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAG                              <  SRR4302540.6467189/94‑1 (MQ=60)
                                                                GGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAG                              >  SRR4302540.1611477/1‑94 (MQ=60)
                                                                  CTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCG                            <  SRR4302540.5658628/94‑1 (MQ=60)
                                                                  CTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCG                            >  SRR4302540.4375118/1‑94 (MQ=60)
                                                                  CTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCG                            <  SRR4302540.6271596/94‑1 (MQ=60)
                                                                   TTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGT                           <  SRR4302540.5983911/94‑1 (MQ=60)
                                                                    TTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTT                          >  SRR4302540.4984643/1‑94 (MQ=60)
                                                                    TTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTT                          <  SRR4302540.3565667/94‑1 (MQ=60)
                                                                     TGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTT                         >  SRR4302540.487815/1‑94 (MQ=60)
                                                                       CAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCG                       <  SRR4302540.129459/94‑1 (MQ=60)
                                                                           ACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACT                   >  SRR4302540.9099602/1‑94 (MQ=60)
                                                                            CAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTG                  >  SRR4302540.3719169/1‑94 (MQ=60)
                                                                            CAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTG                  >  SRR4302540.9546608/1‑94 (MQ=60)
                                                                                 GGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAAT             <  SRR4302540.6507024/94‑1 (MQ=60)
                                                                                  GGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATC            <  SRR4302540.3582205/94‑1 (MQ=60)
                                                                                   GGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCG           <  SRR4302540.3614106/94‑1 (MQ=60)
                                                                                      ACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAG        >  SRR4302540.7111828/1‑94 (MQ=60)
                                                                                       CCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGT       <  SRR4302540.9138282/94‑1 (MQ=60)
                                                                                       CCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGT       >  SRR4302540.5126113/1‑94 (MQ=60)
                                                                                       CCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGT       <  SRR4302540.1132251/94‑1 (MQ=60)
                                                                                       CCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGT       <  SRR4302540.8582376/94‑1 (MQ=60)
                                                                                        CGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTG      >  SRR4302540.3319091/1‑94 (MQ=60)
                                                                                        CGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTG      <  SRR4302540.3977655/94‑1 (MQ=60)
                                                                                        CGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGAG      >  SRR4302540.3319092/1‑94 (MQ=60)
                                                                                         GGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGT     <  SRR4302540.9663109/94‑1 (MQ=60)
                                                                                           TGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTC   >  SRR4302540.402386/1‑94 (MQ=60)
                                                                                            GGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCG  >  SRR4302540.1992783/1‑94 (MQ=60)
                                                                                            GGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCG  <  SRR4302540.4040858/94‑1 (MQ=60)
                                                                                            GGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCG  <  SRR4302540.760782/94‑1 (MQ=60)
                                                                                             |                                                                                            
TGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCG  >  NC_000913/1191310‑1191495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑