Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,191,403	A→G	V151A (GTC→GCC)	ycfC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,191,403	0	A	G	99.6%	736.7 / NA	234	V151A (GTC→GCC)	ycfC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (97/135); minor base C (0/1); total (97/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

CAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGT  >  NC_000913/1191313‑1191492

cAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGcc                                                                                         <  2:5866401/93‑1 (MQ=255)

cAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGcc                                                                                         >  1:346030/1‑93 (MQ=255)

 agagCGCGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCt                                                                                        <  2:3892900/93‑1 (MQ=255)

 agagCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCt                                                                                        >  2:2612904/1‑93 (MQ=255)

  gagCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTg                                                                                       >  1:5496083/1‑93 (MQ=255)

  gagCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTg                                                                                       >  1:1416200/1‑93 (MQ=255)

    gCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGaa                                                                                     <  1:60151/93‑1 (MQ=255)

     cACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAAt                                                                                    <  2:2769676/93‑1 (MQ=255)

     cACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAAt                                                                                    >  1:3333999/1‑93 (MQ=255)

      aCGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATg                                                                                   <  1:1894565/93‑1 (MQ=255)

       cggcggCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATgc                                                                                  >  2:4730924/1‑93 (MQ=255)

        ggcggcGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATgcg                                                                                 >  2:3925325/1‑93 (MQ=255)

         gcggcgCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTACACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATgcgc                                                                                <  2:3821448/93‑1 (MQ=255)

          cggcgCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGcgcg                                                                               <  2:863624/93‑1 (MQ=255)

          cggcgCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGcgcg                                                                               <  2:2783447/93‑1 (MQ=255)

          cggcgCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGcgcg                                                                               <  1:2066833/93‑1 (MQ=255)

           ggcgCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCgg                                                                              <  1:4666351/93‑1 (MQ=255)

            gcgcGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCggg                                                                             >  1:2081278/1‑93 (MQ=255)

              gcgAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGGCTGAATGCGCGGGcc                                                                           >  2:5997370/1‑93 (MQ=255)

              gcgAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGcc                                                                           <  2:1417223/93‑1 (MQ=255)

              gcgAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGcc                                                                           <  2:1924263/93‑1 (MQ=255)

               cgAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCa                                                                          >  1:6266893/1‑93 (MQ=255)

               cgAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCa                                                                          >  2:3345618/1‑93 (MQ=255)

                gAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCaa                                                                         <  2:1264677/93‑1 (MQ=255)

                 aaTGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAg                                                                        <  2:1511414/93‑1 (MQ=255)

                 aaTGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAg                                                                        <  1:4012172/93‑1 (MQ=255)

                   tgcctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCg                                                                      <  1:3424770/93‑1 (MQ=255)

                   tgcctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCg                                                                      <  1:3555209/93‑1 (MQ=255)

                   tgcctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCg                                                                      <  1:593070/93‑1 (MQ=255)

                     cctgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCggg                                                                    >  1:2349840/1‑93 (MQ=255)

                       tgccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCt                                                                  >  2:3528571/1‑93 (MQ=255)

                         gcagcagGGTTGCGCGAACTTCCGCCTGCACTTGTGCGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGTGCGGGCCAAGCGGGCTaa                                                                <  2:496113/92‑1 (MQ=255)

                         ccAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGCATGCGCGGGCCAAGAGGGCTaa                                                                >  2:319402/1‑93 (MQ=255)

                            gcagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATca                                                             >  2:4649362/1‑93 (MQ=255)

                             cagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATcac                                                            <  1:4410195/93‑1 (MQ=255)

                             cagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATcac                                                            <  2:5673981/93‑1 (MQ=255)

                             cagGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATcac                                                            <  2:1588354/93‑1 (MQ=255)

                              agGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATcaca                                                           <  1:4093242/93‑1 (MQ=255)

                              agGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATcaca                                                           >  2:3003370/1‑93 (MQ=255)

                               gggTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACAt                                                          >  1:3750077/1‑93 (MQ=255)

                               gggTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACAt                                                          >  1:2110346/1‑93 (MQ=255)

                                 gTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCa                                                        <  2:5413373/93‑1 (MQ=255)

                                  ttGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCaa                                                       >  2:1058204/1‑93 (MQ=255)

                                    gcgcGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACa                                                     <  2:3078705/93‑1 (MQ=255)

                                    gcgcGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACa                                                     >  2:5579654/1‑93 (MQ=255)

                                    gcgcGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACa                                                     <  1:3321907/93‑1 (MQ=255)

                                    gcgcGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACa                                                     <  1:2563836/93‑1 (MQ=255)

                                      gcgAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACata                                                   <  2:22860/93‑1 (MQ=255)

                                        gAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACataga                                                 <  2:3848403/93‑1 (MQ=255)

                                         aaCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACatagat                                                >  2:4996142/1‑93 (MQ=255)

                                          aCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACatagata                                               <  1:6250011/93‑1 (MQ=255)

                                          aCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACatagata                                               <  2:3121134/93‑1 (MQ=255)

                                          aCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACatagata                                               >  1:4822263/1‑93 (MQ=255)

                                          aCTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACatagata                                               <  1:2934948/93‑1 (MQ=255)

                                             ttCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATagca                                            <  1:4616633/93‑1 (MQ=255)

                                                gCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGcc                                         >  1:5914304/1‑93 (MQ=255)

                                                gCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGcc                                         >  1:1515628/1‑93 (MQ=255)

                                                 ccTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCa                                        <  1:305710/93‑1 (MQ=255)

                                                 ccTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCa                                        >  2:1763943/1‑93 (MQ=255)

                                                 ccTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCa                                        <  2:510440/93‑1 (MQ=255)

                                                 ccTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCa                                        <  2:204978/93‑1 (MQ=255)

                                                  cTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCAt                                       <  1:2619966/93‑1 (MQ=255)

                                                  cTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCAt                                       >  1:5663095/1‑93 (MQ=255)

                                                   tGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATc                                      <  1:4181911/93‑1 (MQ=255)

                                                        ttGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCt                                 >  2:2398648/1‑93 (MQ=255)

                                                          gtgGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtca                               <  1:539011/93‑1 (MQ=255)

                                                           tgGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtcat                              >  1:604023/1‑93 (MQ=255)

                                                           tgGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtcat                              >  2:4147610/1‑93 (MQ=255)

                                                            gggCTTTGCAGTGCAGCAGGGGAACCGGTCGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtcatc                             <  2:6189806/93‑1 (MQ=255)

                                                            gggCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtcatc                             >  1:2219253/1‑93 (MQ=255)

                                                            gggCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCtcatc                             <  2:772809/93‑1 (MQ=255)

                                                              gCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAg                           >  1:1167159/1‑93 (MQ=255)

                                                              gCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAg                           <  1:330896/93‑1 (MQ=255)

                                                               cTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGc                          >  1:100971/1‑93 (MQ=255)

                                                                tttGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCg                         >  2:3451013/1‑93 (MQ=255)

                                                                 ttGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGt                        <  1:5098549/93‑1 (MQ=255)

                                                                  tGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGtt                       <  1:5825914/93‑1 (MQ=255)

                                                                   gCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGttt                      <  2:5156252/93‑1 (MQ=255)

                                                                   gCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGttt                      <  2:42168/93‑1 (MQ=255)

                                                                    cAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTc                     <  2:1978446/93‑1 (MQ=255)

                                                                      gTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCgg                   >  2:1174537/1‑93 (MQ=255)

                                                                        aCAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGAc                 <  2:4471451/93‑1 (MQ=255)

                                                                        aCAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGAc                 >  2:821115/1‑93 (MQ=255)

                                                                            cagGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTa             <  1:1823119/93‑1 (MQ=255)

                                                                             agGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaa            >  1:3693187/1‑93 (MQ=255)

                                                                             agGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaa            >  1:3953875/1‑93 (MQ=255)

                                                                              ggggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaaa           >  2:3960470/1‑93 (MQ=255)

                                                                              ggggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaaa           <  1:3853534/93‑1 (MQ=255)

                                                                              ggggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaaa           >  1:5262021/1‑93 (MQ=255)

                                                                              ggggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaaa           >  1:163340/1‑93 (MQ=255)

                                                                              ggggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaaa           >  2:5729813/1‑93 (MQ=255)

                                                                              ggggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaaa           >  1:4388680/1‑93 (MQ=255)

                                                                              ggggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTaaa           <  1:5048760/93‑1 (MQ=255)

                                                                               gggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAAt          <  1:2850423/93‑1 (MQ=255)

                                                                                ggAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATc         <  1:5699304/93‑1 (MQ=255)

                                                                                  aaCCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGa       <  1:3138790/93‑1 (MQ=255)

                                                                                    ccGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAg     <  1:3162277/93‑1 (MQ=255)

                                                                                      ggTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAgtg   <  1:1332246/93‑1 (MQ=255)

                                                                                      ggTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAgtg   <  2:3267386/93‑1 (MQ=255)

                                                                                       gTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAgtgt  >  2:3664464/1‑93 (MQ=255)

CAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGT  >  NC_000913/1191313‑1191492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGA  >  NC_000913/1191310‑1191496

TCCCAGAGCCCGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGG                                                                                               <  SRR4302533.3843660/94‑1 (MQ=60)

  CCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCC                                                                                             >  SRR4302533.348142/1‑94 (MQ=60)

  CCACAGCACGGCGGGGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGGGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCC                                                                                             <  SRR4302533.2229739/94‑1 (MQ=60)

   CAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCT                                                                                            <  SRR4302533.5719012/94‑1 (MQ=60)

   CAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCT                                                                                            >  SRR4302533.5381741/1‑94 (MQ=60)

    AGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTG                                                                                           >  SRR4302533.5529233/1‑94 (MQ=60)

    AGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTG                                                                                           >  SRR4302533.1424767/1‑94 (MQ=60)

    AGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTG                                                                                           >  SRR4302533.3511519/1‑94 (MQ=60)

    AGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTG                                                                                           >  SRR4302533.1694557/1‑94 (MQ=60)

      agccgggcgcCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGACCCGGTGGCCTGAA                                                                                         <  SRR4302533.3668759/84‑1 (MQ=60)

       GCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAAT                                                                                        <  SRR4302533.60543/94‑1 (MQ=60)

       GCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAAT                                                                                        >  SRR4302533.3354598/1‑94 (MQ=60)

       GCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAAT                                                                                        <  SRR4302533.1610029/94‑1 (MQ=60)

       GCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAAT                                                                                        >  SRR4302533.4695671/1‑94 (MQ=60)

       GCACGGCGGCGCGAATGCCTGCAAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAAT                                                                                        <  SRR4302533.6202372/94‑1 (MQ=60)

         ACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGC                                                                                      <  SRR4302533.1906131/94‑1 (MQ=60)

          CGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCG                                                                                     <  SRR4302533.4029311/94‑1 (MQ=60)

            GCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCG                                                                                   <  SRR4302533.502199/94‑1 (MQ=60)

             GGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGG                                                                                  <  SRR4302533.595984/94‑1 (MQ=60)

             CGTCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGG                                                                                  <  SRR4302533.2248468/94‑1 (MQ=60)

             CGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGG                                                                                  <  SRR4302533.2079568/94‑1 (MQ=60)

              GGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGG                                                                                 <  SRR4302533.4694623/94‑1 (MQ=60)

              GGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGG                                                                                 >  SRR4302533.2094085/1‑94 (MQ=60)

                 GCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCA                                                                              >  SRR4302533.5526858/1‑94 (MQ=60)

                 GCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCA                                                                              >  SRR4302533.6304337/1‑94 (MQ=60)

                  CGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAA                                                                             >  SRR4302533.3379602/1‑94 (MQ=60)

                  CGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAA                                                                             >  SRR4302533.4620617/1‑94 (MQ=60)

                   GAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAG                                                                            <  SRR4302533.3208141/94‑1 (MQ=60)

                    AATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGC                                                                           <  SRR4302533.4036604/94‑1 (MQ=60)

                     ATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCG                                                                          >  SRR4302533.1293125/1‑94 (MQ=60)

                     ATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCG                                                                          <  SRR4302533.2715597/94‑1 (MQ=60)

                      TGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGG                                                                         <  SRR4302533.596570/94‑1 (MQ=60)

                      TGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGG                                                                         <  SRR4302533.3445928/94‑1 (MQ=60)

                      TGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGG                                                                         <  SRR4302533.2501757/94‑1 (MQ=60)

                      TGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGG                                                                         >  SRR4302533.209415/1‑94 (MQ=60)

                      TGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGG                                                                         <  SRR4302533.3577120/94‑1 (MQ=60)

                      TGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGCCCTGAATGCGCGGGCCAAGCGG                                                                         <  SRR4302533.6289793/94‑1 (MQ=60)

                       GCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGG                                                                        >  SRR4302533.2364375/1‑94 (MQ=60)

                         CTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCT                                                                      >  SRR4302533.4691689/1‑94 (MQ=60)

                           GCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAA                                                                    >  SRR4302533.2988092/1‑94 (MQ=60)

                           GCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAA                                                                    >  SRR4302533.5090824/1‑94 (MQ=60)

                            CCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAAT                                                                   >  SRR4302533.3097387/1‑94 (MQ=60)

                            CCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAAT                                                                   >  SRR4302533.1424747/1‑94 (MQ=60)

                             CAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCAAAGCGGGCTAATC                                                                  >  SRR4302533.3622147/1‑94 (MQ=60)

                              AGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCA                                                                 <  SRR4302533.3458953/94‑1 (MQ=60)

                                CAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACA                                                               <  SRR4302533.4436830/94‑1 (MQ=60)

                                 AGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACAT                                                              <  SRR4302533.4118158/94‑1 (MQ=60)

                                 AGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACAT                                                              <  SRR4302533.4091297/94‑1 (MQ=60)

                                 AGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACAT                                                              >  SRR4302533.3773068/1‑94 (MQ=60)

                                 AGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACAT                                                              >  SRR4302533.2123361/1‑94 (MQ=60)

                                 AGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACAT                                                              <  SRR4302533.2127296/94‑1 (MQ=60)

                                  GGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATC                                                             >  SRR4302533.1109070/1‑94 (MQ=60)

                                     TTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAAC                                                          <  SRR4302533.4275237/94‑1 (MQ=60)

                                      TGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACA                                                         <  SRR4302533.2110090/94‑1 (MQ=60)

                                       GCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACAT                                                        <  SRR4302533.3342425/94‑1 (MQ=60)

                                       GCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACAT                                                        <  SRR4302533.4003810/94‑1 (MQ=60)

                                       GCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACAT                                                        <  SRR4302533.2579616/94‑1 (MQ=60)

                                       GCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACAT                                                        <  SRR4302533.2071640/94‑1 (MQ=60)

                                        CGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATA                                                       >  SRR4302533.577495/1‑94 (MQ=60)

                                        CGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATA                                                       >  SRR4302533.34192/1‑94 (MQ=60)

                                          CGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGA                                                     <  SRR4302533.4436455/94‑1 (MQ=60)

                                           GAACTTTCGCCTGCACTTGTGGTCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGAT                                                    <  SRR4302533.6001130/94‑1 (MQ=60)

                                           GAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGAT                                                    <  SRR4302533.3545501/94‑1 (MQ=60)

                                           GAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGAT                                                    <  SRR4302533.1614335/94‑1 (MQ=60)

                                           GAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGAT                                                    <  SRR4302533.1898038/94‑1 (MQ=60)

                                            AACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATA                                                   >  SRR4302533.4851482/1‑94 (MQ=60)

                                            AACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATA                                                   <  SRR4302533.3831424/94‑1 (MQ=60)

                                             ACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAG                                                  <  SRR4302533.6287404/94‑1 (MQ=60)

                                             ACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAG                                                  >  SRR4302533.3790180/1‑94 (MQ=60)

                                             ACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAG                                                  <  SRR4302533.2952892/94‑1 (MQ=60)

                                              CTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGC                                                 >  SRR4302533.5780187/1‑94 (MQ=60)

                                               TTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCA                                                <  SRR4302533.4122698/94‑1 (MQ=60)

                                                TTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAG                                               <  SRR4302533.4644589/94‑1 (MQ=60)

                                                 TCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGC                                              <  SRR4302533.4272942/94‑1 (MQ=60)

                                                  CGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCC                                             >  SRR4302533.5950044/1‑94 (MQ=60)

                                                  CGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCC                                             >  SRR4302533.1524756/1‑94 (MQ=60)

                                                    CCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCAT                                           >  SRR4302533.5697309/1‑94 (MQ=60)

                                                    CCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCAT                                           <  SRR4302533.307579/94‑1 (MQ=60)

                                                    CCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCAT                                           >  SRR4302533.4972550/1‑94 (MQ=60)

                                                     CTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATC                                          >  SRR4302533.3345166/1‑94 (MQ=60)

                                                     CTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATC                                          <  SRR4302533.2636048/94‑1 (MQ=60)

                                                     CTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATC                                          <  SRR4302533.3116986/94‑1 (MQ=60)

                                                     CTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATC                                          >  SRR4302533.37392/1‑94 (MQ=60)

                                                      TGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCG                                         <  SRR4302533.4207239/94‑1 (MQ=60)

                                                      TGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCG                                         >  SRR4302533.4573942/1‑94 (MQ=60)

                                                        CACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCG                                       <  SRR4302533.261971/94‑1 (MQ=60)

                                                        CACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCG                                       >  SRR4302533.3341319/1‑94 (MQ=60)

                                                         ACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGC                                      >  SRR4302533.1496153/1‑94 (MQ=60)

                                                             GTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCAT                                  <  SRR4302533.542216/94‑1 (MQ=60)

                                                             GTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCAT                                  >  SRR4302533.5626028/1‑94 (MQ=60)

                                                             GTGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCAT                                  >  SRR4302533.607571/1‑94 (MQ=60)

                                                              TGGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATC                                 >  SRR4302533.2232954/1‑94 (MQ=60)

                                                               GGGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCA                                <  SRR4302533.3249391/94‑1 (MQ=60)

                                                                GGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAG                               <  SRR4302533.2120152/94‑1 (MQ=60)

                                                                GGCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAG                               >  SRR4302533.1174163/1‑94 (MQ=60)

                                                                 GCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGC                              <  SRR4302533.332920/94‑1 (MQ=60)

                                                                 GCTTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGC                              >  SRR4302533.101598/1‑94 (MQ=60)

                                                                   TTTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGT                            <  SRR4302533.2404873/94‑1 (MQ=60)

                                                                   TATGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCCGCGT                            >  SRR4302533.5279512/1‑94 (MQ=60)

                                                                    TTGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTT                           <  SRR4302533.5129352/94‑1 (MQ=60)

                                                                     TGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTT                          <  SRR4302533.1926378/94‑1 (MQ=60)

                                                                     TGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTT                          <  SRR4302533.6084503/94‑1 (MQ=60)

                                                                     TGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTT                          <  SRR4302533.5861117/94‑1 (MQ=60)

                                                                     TGCAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCAAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTT                          <  SRR4302533.2873135/94‑1 (MQ=60)

                                                                       CAGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCG                        >  SRR4302533.1408901/1‑94 (MQ=60)

                                                                        AGTACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGG                       <  SRR4302533.3969393/94‑1 (MQ=60)

                                                                          TACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGAC                     <  SRR4302533.3181453/94‑1 (MQ=60)

                                                                          TACAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGAC                     <  SRR4302533.5290290/94‑1 (MQ=60)

                                                                            CAGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTG                   <  SRR4302533.6064548/94‑1 (MQ=60)

                                                                             AGCAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGG                  >  SRR4302533.265726/1‑94 (MQ=60)

                                                                               CAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAA                >  SRR4302533.3977982/1‑94 (MQ=60)

                                                                               CAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAA                <  SRR4302533.1834256/94‑1 (MQ=60)

                                                                               CAGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAA                >  SRR4302533.3715875/1‑94 (MQ=60)

                                                                                AGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAA               >  SRR4302533.164380/1‑94 (MQ=60)

                                                                                AGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAA               >  SRR4302533.4415177/1‑94 (MQ=60)

                                                                                AGGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAA               >  SRR4302533.5293610/1‑94 (MQ=60)

                                                                                 GGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAAT              <  SRR4302533.5079298/94‑1 (MQ=60)

                                                                                 GGGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAAT              <  SRR4302533.3877073/94‑1 (MQ=60)

                                                                                  GGGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATC             <  SRR4302533.2867961/94‑1 (MQ=60)

                                                                                   GGAACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCG            <  SRR4302533.5733724/94‑1 (MQ=60)

                                                                                     AACCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAA          <  SRR4302533.3158085/94‑1 (MQ=60)

                                                                                       CCGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGT        <  SRR4302533.3181739/94‑1 (MQ=60)

                                                                                        CGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTG       <  SRR4302533.6038394/94‑1 (MQ=60)

                                                                                        CGGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTG       <  SRR4302533.5888682/94‑1 (MQ=60)

                                                                                         GGTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGT      <  SRR4302533.1340241/94‑1 (MQ=60)

                                                                                          GTGGCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTT     <  SRR4302533.5647156/94‑1 (MQ=60)

                                                                                             GCCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGA  >  SRR4302533.36689/1‑94 (MQ=60)

TGCCAGAGCACGGCGGCGCGAATGCCTGCCAGCAGGGTTGCGCGAACTTTCGCCTGCACTTGTGGGCTTTGCAGTACAGCAGGGGAACCGGTGACCTGAATGCGCGGGCCAAGCGGGCTAATCACATCAACATAGATAGCAGCCATCGCGCTCATCAGCGTTTCGGACTGTAAATCGAAGTGTTCGA  >  NC_000913/1191310‑1191496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`