Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,249,670 A→G V131A (GTC→GCC)  b1200 ← putative dihydroxyacetone kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,249,6700AG99.5% 677.4 / NA 213V131A (GTC→GCC) b1200putative dihydroxyacetone kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (108/104);  minor base C (1/0);  total (109/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

CCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATC  >  NC_000913/1249580‑1249759
                                                                                          |                                                                                         
 cTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCca                                                                                        <  1:942082/93‑1 (MQ=255)
 cTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCca                                                                                        >  2:185148/1‑93 (MQ=255)
 cTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCca                                                                                        >  2:767985/1‑93 (MQ=255)
  tACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCcac                                                                                       <  2:8212482/93‑1 (MQ=255)
  tACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCcac                                                                                       >  1:5062559/1‑93 (MQ=255)
  tACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCcac                                                                                       >  1:2188961/1‑93 (MQ=255)
   aCGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCcaca                                                                                      >  2:7813574/1‑93 (MQ=255)
   aCGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCcaca                                                                                      <  1:7489750/93‑1 (MQ=255)
    cGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAg                                                                                     <  2:3508410/93‑1 (MQ=255)
     gAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAgc                                                                                    >  1:1617189/1‑92 (MQ=255)
        tttttCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGt                                                                                 >  2:2970334/1‑93 (MQ=255)
        tttttCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGt                                                                                 >  1:5464570/1‑93 (MQ=255)
         ttttCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTc                                                                                >  2:1349438/1‑93 (MQ=255)
         ttttCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTc                                                                                >  1:3142305/1‑93 (MQ=255)
          tttCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCa                                                                               >  2:456043/1‑93 (MQ=255)
          tttCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCa                                                                               >  1:61595/1‑93 (MQ=255)
          tttCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCa                                                                               >  1:1335495/1‑93 (MQ=255)
              aaTTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACttt                                                                           <  1:8238929/93‑1 (MQ=255)
              aaTTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACttt                                                                           >  2:4447551/1‑93 (MQ=255)
                ttAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACTGTGGTCACTTTTa                                                                         <  2:8536932/93‑1 (MQ=255)
                ttAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTa                                                                         <  2:6868433/93‑1 (MQ=255)
                  aaTACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAcg                                                                       >  2:6813874/1‑93 (MQ=255)
                  aaTACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACCTTTAcg                                                                       >  2:6813876/1‑93 (MQ=255)
                   aTACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAcgc                                                                      <  2:1581173/93‑1 (MQ=255)
                   aTACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAcgc                                                                      >  1:7586216/1‑93 (MQ=255)
                       ggTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCt                                                                  <  1:2244929/93‑1 (MQ=255)
                       ggTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCt                                                                  <  2:4305832/93‑1 (MQ=255)
                         tgtTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTAt                                                                <  2:4023989/93‑1 (MQ=255)
                          gtTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATc                                                               <  1:528375/93‑1 (MQ=255)
                          gtTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATc                                                               <  2:6541776/93‑1 (MQ=255)
                              gCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGc                                                           >  2:2960958/1‑93 (MQ=255)
                              gCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTAACTTTTACGCCGCTATCGTGc                                                           >  2:2380090/1‑93 (MQ=255)
                                aaCGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAg                                                         >  2:9453389/1‑93 (MQ=255)
                                 aCGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGt                                                        >  2:7557271/1‑93 (MQ=255)
                                    ccgcGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCGGTAAc                                                     >  2:3718769/1‑93 (MQ=255)
                                    ccgcGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAAc                                                     <  2:5572828/93‑1 (MQ=255)
                                    ccgcGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAAc                                                     <  1:2018462/93‑1 (MQ=255)
                                    ccgcGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAAc                                                     >  2:3184575/1‑93 (MQ=255)
                                    ccgcGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAAc                                                     >  1:6926693/1‑93 (MQ=255)
                                     cgcgTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACt                                                    <  1:2256324/93‑1 (MQ=255)
                                       cgTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCg                                                  <  1:6119017/93‑1 (MQ=255)
                                        gTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCgg                                                 <  2:7314190/93‑1 (MQ=255)
                                         tCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGt                                                <  1:2675788/93‑1 (MQ=255)
                                         gcGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGt                                                <  1:7858807/92‑1 (MQ=255)
                                          cGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTc                                               <  1:3873210/93‑1 (MQ=255)
                                             ccGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCt                                            >  2:9541447/1‑93 (MQ=255)
                                             ccGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCt                                            <  1:8593846/93‑1 (MQ=255)
                                               ggCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGt                                          <  1:9462818/93‑1 (MQ=255)
                                                 cAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGttt                                        <  1:3619933/93‑1 (MQ=255)
                                                   gTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCa                                      >  1:4844569/1‑93 (MQ=255)
                                                    tataAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTGTACGCCGCTACCGTGCAGTAACTCGGGCGCTGTTTCaa                                     >  1:7718268/1‑93 (MQ=255)
                                                     ataAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCaaa                                    >  1:8456448/1‑93 (MQ=255)
                                                     ataAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCaaa                                    <  1:7784165/93‑1 (MQ=255)
                                                     ataAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCaaa                                    >  1:2886349/1‑93 (MQ=255)
                                                     ataAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCaaa                                    >  2:2812381/1‑93 (MQ=255)
                                                     ataAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCaaa                                    <  2:5114842/93‑1 (MQ=255)
                                                      taAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAg                                   >  2:5664042/1‑93 (MQ=255)
                                                        aaGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGtt                                 >  1:3858099/1‑93 (MQ=255)
                                                         aGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTa                                <  1:1900027/93‑1 (MQ=255)
                                                         aGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTa                                <  2:2289924/93‑1 (MQ=255)
                                                          gACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTaa                               >  2:6058600/1‑93 (MQ=255)
                                                            cTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGa                             >  2:8009666/1‑93 (MQ=255)
                                                             tGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGaa                            <  2:9766083/93‑1 (MQ=255)
                                                             tGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGaa                            <  1:6572009/93‑1 (MQ=255)
                                                             tGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGaa                            >  1:1153458/1‑93 (MQ=255)
                                                             tGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGaa                            <  1:4758083/93‑1 (MQ=255)
                                                               tCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAata                          <  2:4099403/93‑1 (MQ=255)
                                                               tCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAata                          <  1:5754915/93‑1 (MQ=255)
                                                                cTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAatat                         <  2:5957851/93‑1 (MQ=255)
                                                                 ttttACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATc                        >  1:2438684/1‑93 (MQ=255)
                                                                 ttttACCACAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATc                        <  1:1539520/93‑1 (MQ=255)
                                                                  tttACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCg                       <  1:4320777/93‑1 (MQ=255)
                                                                    tACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGcc                     <  2:4678628/93‑1 (MQ=255)
                                                                      ccGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCgg                   <  1:9830429/93‑1 (MQ=255)
                                                                      ccGCAACGTCGTCATCAATCGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCgg                   >  1:7961420/1‑93 (MQ=255)
                                                                        gCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGgtg                 <  1:3670882/93‑1 (MQ=255)
                                                                          aaCGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTa               <  1:1754536/93‑1 (MQ=255)
                                                                           aCGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTaa              >  1:6369574/1‑93 (MQ=255)
                                                                           aCGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTaa              >  1:666027/1‑93 (MQ=255)
                                                                           aCGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTaa              >  1:7303240/1‑93 (MQ=255)
                                                                           aCGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTaa              >  2:10017025/1‑93 (MQ=255)
                                                                           aCGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTaa              >  2:4133267/1‑93 (MQ=255)
                                                                           aCGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTaa              <  1:9118602/93‑1 (MQ=255)
                                                                            cgtcgtCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAt             >  1:4597106/1‑93 (MQ=255)
                                                                            cgtcgtCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAt             <  1:887783/93‑1 (MQ=255)
                                                                              tcgtcATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAttt           <  1:5997686/93‑1 (MQ=255)
                                                                                gtcATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAttttt         >  2:5377419/1‑93 (MQ=255)
                                                                                gtcATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAttttt         <  2:3481204/93‑1 (MQ=255)
                                                                                 tcatcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTg        >  1:853449/1‑93 (MQ=255)
                                                                                 tcatcaATGGCCACAGTGGGCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGGAATTTTTg        >  2:677307/1‑93 (MQ=255)
                                                                                 gcatcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTg        <  1:7333793/92‑1 (MQ=255)
                                                                                  catcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGa       >  1:9928220/1‑93 (MQ=255)
                                                                                  catcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGa       >  2:7859651/1‑93 (MQ=255)
                                                                                   atcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGAt      >  2:2544248/1‑93 (MQ=255)
                                                                                   atcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGAt      >  2:3643703/1‑93 (MQ=255)
                                                                                   atcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGAt      >  1:8021230/1‑93 (MQ=255)
                                                                                      aaTGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAAt   <  1:3049787/93‑1 (MQ=255)
                                                                                      aaTGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAAt   <  2:2726368/93‑1 (MQ=255)
                                                                                       aTGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATc  <  1:4982470/93‑1 (MQ=255)
                                                                                       aTGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATc  >  2:42608/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATC  >  NC_000913/1249580‑1249759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACA  >  NC_000913/1249577‑1249763
                                                                                             |                                                                                             
GCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGG                                                                                               >  SRR4302540.6343432/1‑94 (MQ=60)
 CGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGC                                                                                              >  SRR4302540.4858036/1‑94 (MQ=60)
  GCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATCAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCC                                                                                             >  SRR4302540.2533337/1‑94 (MQ=60)
    CTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCAC                                                                                           >  SRR4302540.5117294/1‑94 (MQ=60)
    CTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCAC                                                                                           <  SRR4302540.951920/94‑1 (MQ=60)
    CTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCAC                                                                                           >  SRR4302540.2213358/1‑94 (MQ=60)
    CTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCAC                                                                                           >  SRR4302540.2212595/1‑94 (MQ=60)
    CTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCAC                                                                                           >  SRR4302540.785026/1‑94 (MQ=60)
      ACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAG                                                                                         <  SRR4302540.7573365/94‑1 (MQ=60)
      ACGAGTCTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCTTCAATGGCCACAG                                                                                         >  SRR4302540.9068971/1‑94 (MQ=60)
       CGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGC                                                                                        >  SRR4302540.1634925/1‑94 (MQ=60)
        GAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTG                                                                                       <  SRR4302540.1549332/94‑1 (MQ=60)
        GAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTG                                                                                       <  SRR4302540.530820/94‑1 (MQ=60)
         AGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGG                                                                                      <  SRR4302540.8934948/94‑1 (MQ=60)
          GTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGT                                                                                     >  SRR4302540.5524717/1‑94 (MQ=60)
          GTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATAGCCACAGTGGT                                                                                     >  SRR4302540.391108/1‑94 (MQ=60)
           TTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTC                                                                                    >  SRR4302540.3176561/1‑94 (MQ=60)
            TTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCA                                                                                   >  SRR4302540.5299543/1‑94 (MQ=60)
            TTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCA                                                                                   >  SRR4302540.1349474/1‑94 (MQ=60)
            TTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCA                                                                                   >  SRR4302540.62217/1‑94 (MQ=60)
             TTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCAC                                                                                  <  SRR4302540.3511261/94‑1 (MQ=60)
              TTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACT                                                                                 >  SRR4302540.4882756/1‑94 (MQ=60)
                CAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTT                                                                               >  SRR4302540.2459920/1‑94 (MQ=60)
                 AATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTT                                                                              <  SRR4302540.8334683/94‑1 (MQ=60)
                    TAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACG                                                                           <  SRR4302540.6022948/94‑1 (MQ=60)
                     AATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGC                                                                          >  SRR4302540.7671404/1‑94 (MQ=60)
                     AATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGC                                                                          >  SRR4302540.7671403/1‑94 (MQ=60)
                      ATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGCCCACAGTGGTCACTTTTACTCC                                                                         >  SRR4302540.6175661/1‑94 (MQ=60)
                         CGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCT                                                                      >  SRR4302540.3567797/1‑94 (MQ=60)
                         CGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCT                                                                      <  SRR4302540.9571704/94‑1 (MQ=60)
                          cgtgttggaagaGCCGCGTCGCCCGGGAGTATAAAGACTGTCTTTTGACGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTA                                                                     <  SRR4302540.2269054/82‑1 (MQ=60)
                          GGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTA                                                                     <  SRR4302540.2269052/94‑1 (MQ=60)
                            TGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATC                                                                   <  SRR4302540.1609503/94‑1 (MQ=60)
                             GTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCG                                                                  <  SRR4302540.534089/94‑1 (MQ=60)
                              TTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGT                                                                 <  SRR4302540.8288965/94‑1 (MQ=60)
                                  CAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAG                                                             <  SRR4302540.4709803/94‑1 (MQ=60)
                                  CAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAG                                                             <  SRR4302540.739520/94‑1 (MQ=60)
                                   AACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGT                                                            >  SRR4302540.6214748/1‑94 (MQ=60)
                                   AACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGA                                                            >  SRR4302540.2157974/1‑94 (MQ=60)
                                      GCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAAC                                                         >  SRR4302540.7003782/1‑94 (MQ=60)
                                       CCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACT                                                        <  SRR4302540.2040279/94‑1 (MQ=60)
                                        CGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTC                                                       <  SRR4302540.2280539/94‑1 (MQ=60)
                                        CGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTC                                                       >  SRR4302540.1813972/1‑94 (MQ=60)
                                        CGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTC                                                       >  SRR4302540.1813677/1‑94 (MQ=60)
                                         gcggcagcctGCAGTATAAAGACTGTCTTTTACCGCAACGCCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCG                                                      <  SRR4302540.1171313/84‑1 (MQ=60)
                                          CGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGG                                                     <  SRR4302540.6187165/94‑1 (MQ=60)
                                            TCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTC                                                   >  SRR4302540.7972429/1‑94 (MQ=60)
                                            TCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTC                                                   <  SRR4302540.2704753/94‑1 (MQ=60)
                                            GCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTC                                                   <  SRR4302540.7950634/94‑1 (MQ=60)
                                             CGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCG                                                  <  SRR4302540.3915309/94‑1 (MQ=60)
                                              GCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGC                                                 >  SRR4302540.9361304/1‑94 (MQ=60)
                                              GCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGC                                                 >  SRR4302540.8113980/1‑94 (MQ=60)
                                                CCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTG                                               <  SRR4302540.8693744/94‑1 (MQ=60)
                                                 CGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGT                                              >  SRR4302540.9530599/1‑94 (MQ=60)
                                                  GGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTT                                             <  SRR4302540.9575090/94‑1 (MQ=60)
                                                  GGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTT                                             <  SRR4302540.5789646/94‑1 (MQ=60)
                                                    CAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTC                                           >  SRR4302540.9675753/1‑94 (MQ=60)
                                                    CAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTC                                           >  SRR4302540.1720886/1‑94 (MQ=60)
                                                    CAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTC                                           <  SRR4302540.3659535/94‑1 (MQ=60)
                                                     AGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCA                                          >  SRR4302540.4896465/1‑94 (MQ=60)
                                                      GTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAA                                         >  SRR4302540.7807560/1‑94 (MQ=60)
                                                      GTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAA                                         >  SRR4302540.4481826/1‑94 (MQ=60)
                                                      GTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTGTACGCCGCTACCGTGCAGTAACTCGGGCGCTGTTTCAA                                         >  SRR4302540.7807559/1‑94 (MQ=60)
                                                       TATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAA                                        >  SRR4302540.8554639/1‑94 (MQ=60)
                                                       TATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAA                                        <  SRR4302540.6428585/94‑1 (MQ=60)
                                                       TATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAA                                        >  SRR4302540.2917776/1‑94 (MQ=60)
                                                       TATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAA                                        >  SRR4302540.8554977/1‑94 (MQ=60)
                                                       TATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGGTTCAAA                                        >  SRR4302540.2917487/1‑94 (MQ=60)
                                                        ATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAG                                       <  SRR4302540.7875148/94‑1 (MQ=60)
                                                        ATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATCGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAG                                       >  SRR4302540.6731918/1‑94 (MQ=60)
                                                          AAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTT                                     >  SRR4302540.3900044/1‑94 (MQ=60)
                                                          AAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTT                                     >  SRR4302540.8442363/1‑94 (MQ=60)
                                                           AAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTA                                    <  SRR4302540.4931524/94‑1 (MQ=60)
                                                           AAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTA                                    >  SRR4302540.7112488/1‑94 (MQ=60)
                                                            AGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAA                                   <  SRR4302540.1920809/94‑1 (MQ=60)
                                                            AGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAA                                   <  SRR4302540.7910053/94‑1 (MQ=60)
                                                            AGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAA                                   >  SRR4302540.122652/1‑94 (MQ=60)
                                                               CTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAA                                >  SRR4302540.7420699/1‑94 (MQ=60)
                                                               CTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAA                                <  SRR4302540.2421449/94‑1 (MQ=60)
                                                               CTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAA                                >  SRR4302540.1165467/1‑94 (MQ=60)
                                                                TGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAAT                               <  SRR4302540.6644822/94‑1 (MQ=60)
                                                                TGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAAT                               <  SRR4302540.4808856/94‑1 (MQ=60)
                                                                TGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAAT                               <  SRR4302540.4180976/94‑1 (MQ=60)
                                                                 GTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATA                              <  SRR4302540.6442935/94‑1 (MQ=60)
                                                                  TCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATAT                             <  SRR4302540.5818440/94‑1 (MQ=60)
                                                                  TCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATAT                             <  SRR4302540.5092088/94‑1 (MQ=60)
                                                                   CTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATC                            >  SRR4302540.2464944/1‑94 (MQ=60)
                                                                    TTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCG                           <  SRR4302540.3119587/94‑1 (MQ=60)
                                                                    TTTTACCACAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCG                           <  SRR4302540.1556540/94‑1 (MQ=60)
                                                                     TTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGC                          <  SRR4302540.4367817/94‑1 (MQ=60)
                                                                     TTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGC                          <  SRR4302540.2963704/94‑1 (MQ=60)
                                                                      TTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCC                         <  SRR4302540.6210511/94‑1 (MQ=60)
                                                                      TTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCC                         <  SRR4302540.10001569/94‑1 (MQ=60)
                                                                        ACCGCAACGTCGTCATCAATCGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGG                       >  SRR4302540.8054203/1‑94 (MQ=60)
                                                                         CCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGT                      <  SRR4302540.9945404/94‑1 (MQ=60)
                                                                           GCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGT                    <  SRR4302540.3711035/94‑1 (MQ=60)
                                                                            CAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTA                   <  SRR4302540.5495331/94‑1 (MQ=60)
                                                                             AACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAA                  >  SRR4302540.1949586/1‑94 (MQ=60)
                                                                             AACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAA                  >  SRR4302540.7384763/1‑94 (MQ=60)
                                                                             AACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAA                  >  SRR4302540.3710104/1‑94 (MQ=60)
                                                                             AACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAA                  >  SRR4302540.673173/1‑94 (MQ=60)
                                                                             AACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAA                  >  SRR4302540.6440504/1‑94 (MQ=60)
                                                                             AACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAA                  >  SRR4302540.10102694/1‑94 (MQ=60)
                                                                             AACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAA                  <  SRR4302540.1773547/94‑1 (MQ=60)
                                                                              ACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAT                 <  SRR4302540.9228209/94‑1 (MQ=60)
                                                                              ACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAT                 >  SRR4302540.4646540/1‑94 (MQ=60)
                                                                              ACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAT                 <  SRR4302540.6842036/94‑1 (MQ=60)
                                                                               CGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATT                <  SRR4302540.897210/94‑1 (MQ=60)
                                                                                GTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTT               >  SRR4302540.7188276/1‑94 (MQ=60)
                                                                                 TCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTT              <  SRR4302540.6064152/94‑1 (MQ=60)
                                                                                   GTCATCAATGGCCACAGTGGTCAGTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTG            >  SRR4302540.4556285/1‑94 (MQ=60)
                                                                                   GTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTG            >  SRR4302540.8385582/1‑94 (MQ=60)
                                                                                   GTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTG            >  SRR4302540.8639992/1‑94 (MQ=60)
                                                                                   GTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTG            >  SRR4302540.862514/1‑94 (MQ=60)
                                                                                   GTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTG            <  SRR4302540.761961/94‑1 (MQ=60)
                                                                                    TCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGA           >  SRR4302540.10044123/1‑94 (MQ=60)
                                                                                    GCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGA           <  SRR4302540.7415650/94‑1 (MQ=60)
                                                                                     CATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGAT          >  SRR4302540.8114523/1‑94 (MQ=60)
                                                                                      ATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATA         >  SRR4302540.3713354/1‑94 (MQ=60)
                                                                                         AATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATC      <  SRR4302540.3083247/94‑1 (MQ=60)
                                                                                          ATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTgaaacaagc     >  SRR4302540.1214089/1‑85 (MQ=60)
                                                                                          ATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCA     <  SRR4302540.5036415/94‑1 (MQ=60)
                                                                                           TGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAA    <  SRR4302540.9838417/94‑1 (MQ=60)
                                                                                            GGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAAC   >  SRR4302540.4134146/1‑94 (MQ=60)
                                                                                             GCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACA  <  SRR4302540.918223/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACA  >  NC_000913/1249577‑1249763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑