Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,261,945	A→G	R52R (CGT→CGC)	ychB ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,945	0	A	G	99.7%	904.8 / NA	282	R52R (CGT→CGC)	ychB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (188/93); minor base C (0/1); total (188/94)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

CCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATC  >  NC_000913/1261855‑1262034

cccGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCg                                                                                         >  2:2710611/1‑93 (MQ=255)

   gCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCCTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAat                                                                                      >  2:9776123/1‑93 (MQ=255)

    cTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAata                                                                                     <  2:1394751/93‑1 (MQ=255)

    cTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAata                                                                                     <  2:5221036/93‑1 (MQ=255)

     tGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAatat                                                                                    <  1:1239598/93‑1 (MQ=255)

     tGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAatat                                                                                    <  1:9467102/93‑1 (MQ=255)

      gTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATc                                                                                   >  1:7674003/1‑93 (MQ=255)

      gTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATc                                                                                   <  2:2018388/93‑1 (MQ=255)

          gCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGTTCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCa                                                                               <  2:922581/93‑1 (MQ=255)

          gCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCa                                                                               >  2:7271985/1‑93 (MQ=255)

          gCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCa                                                                               >  2:5021827/1‑93 (MQ=255)

           ccGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATGTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCAt                                                                              <  2:6619327/93‑1 (MQ=255)

           ccGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCAt                                                                              <  1:1091136/93‑1 (MQ=255)

             gCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCg                                                                            >  2:9387763/1‑93 (MQ=255)

             gCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCg                                                                            >  1:9303799/1‑93 (MQ=255)

              cAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGt                                                                           >  1:6423042/1‑93 (MQ=255)

              cAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCACCTCGt                                                                           >  2:4739995/1‑93 (MQ=255)

               aGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTc                                                                          >  1:3051960/1‑93 (MQ=255)

               aGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTc                                                                          >  2:4812721/1‑93 (MQ=255)

               aGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTc                                                                          >  1:4469722/1‑93 (MQ=255)

                 ttttCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCAc                                                                        >  2:6335625/1‑93 (MQ=255)

                 ttttCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCAc                                                                        >  2:7915758/1‑93 (MQ=255)

                 ttttCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGCCGTTAACAGGCGAATATCCCCATCGTCag                                                                        >  2:4906415/1‑92 (MQ=255)

                  tttCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCAca                                                                       >  2:6916450/1‑92 (MQ=255)

                   ttCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGa                                                                      <  1:565064/93‑1 (MQ=255)

                   ttCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGa                                                                      >  2:864734/1‑93 (MQ=255)

                   ttCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACCGGCGTTAACAGGCGAATATCCCCATCGTCACGa                                                                      >  1:6648266/1‑93 (MQ=255)

                   tccatcaACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGGTAACAGGCGAATATCCCCATCGTCACGa                                                                      >  1:8991620/3‑93 (MQ=255)

                       tcaACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCt                                                                  >  1:3607698/1‑93 (MQ=255)

                         aacaaTCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCa                                                                >  2:5428133/1‑93 (MQ=255)

                           caaTCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAAt                                                              >  2:3058220/1‑93 (MQ=255)

                           caaTCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAAt                                                              <  2:9947156/93‑1 (MQ=255)

                            aaTCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATg                                                             <  2:2537616/93‑1 (MQ=255)

                             aTCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGc                                                            <  2:314850/93‑1 (MQ=255)

                             aTCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGc                                                            >  2:1626189/1‑93 (MQ=255)

                             aTCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGc                                                            <  2:4455291/93‑1 (MQ=255)

                             aTCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGc                                                            <  1:8794843/93‑1 (MQ=255)

                              tCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCt                                                           <  2:2385398/93‑1 (MQ=255)

                              tCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCt                                                           >  1:6477616/1‑93 (MQ=255)

                                gcgcTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGa                                                         >  1:1827021/1‑93 (MQ=255)

                                gcgcTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGa                                                         <  1:3524575/93‑1 (MQ=255)

                                   cTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGCAGCTCAATGCTGATgg                                                      >  2:601936/1‑93 (MQ=255)

                                   cTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATgg                                                      <  1:377188/93‑1 (MQ=255)

                                    tGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGgt                                                     >  2:169248/1‑93 (MQ=255)

                                     gcgcGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGgtg                                                    >  1:5446749/1‑93 (MQ=255)

                                       gcgAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGgtgtg                                                  >  1:2762577/1‑92 (MQ=255)

                                         gAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAGCAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGc                                                >  2:5803842/1‑93 (MQ=255)

                                         gAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGc                                                <  1:8168031/93‑1 (MQ=255)

                                          aaCGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGcc                                               >  2:10032481/1‑93 (MQ=255)

                                           aCGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCg                                              >  1:3879558/1‑93 (MQ=255)

                                            cGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGt                                             >  1:8445396/1‑93 (MQ=255)

                                             gATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTa                                            >  2:7751008/1‑93 (MQ=255)

                                             gATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTa                                            >  1:7087358/1‑93 (MQ=255)

                                              aTCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTaa                                           >  1:484023/1‑93 (MQ=255)

                                              aTCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTaa                                           >  1:6951080/1‑93 (MQ=255)

                                                 aGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAAtaa                                        >  1:7917678/1‑91 (MQ=255)

                                                 aGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCa                                        <  2:3421167/93‑1 (MQ=255)

                                                 aGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCa                                        >  1:6857049/1‑93 (MQ=255)

                                                 aGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCa                                        >  1:3510023/1‑93 (MQ=255)

                                                  ggTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCaa                                       >  2:8342887/1‑93 (MQ=255)

                                                  ggTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCaa                                       >  2:7133248/1‑93 (MQ=255)

                                                  ggTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCaa                                       >  2:4092295/1‑93 (MQ=255)

                                                  ggTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCaa                                       >  2:3979197/1‑93 (MQ=255)

                                                   gTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAg                                      >  2:7489642/1‑93 (MQ=255)

                                                      aTCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGaaa                                   <  1:6256571/93‑1 (MQ=255)

                                                      aTCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGGCACGAAGCTCAATGCTGATGGTGTCGCCTTAATCAAGaaa                                   <  2:8758841/93‑1 (MQ=255)

                                                        cTTCATGTTCCGCGCCTTCAACGGGCGTGAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACt                                 <  1:9651364/93‑1 (MQ=255)

                                                        cTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACt                                 >  2:4178441/1‑93 (MQ=255)

                                                         ttCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTg                                >  1:2360386/1‑93 (MQ=255)

                                                          tCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGa                               <  2:3861317/93‑1 (MQ=255)

                                                          tCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGa                               <  2:9613651/93‑1 (MQ=255)

                                                             tGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAAc                            >  2:1741758/1‑93 (MQ=255)

                                                             tGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAAc                            <  2:1863723/93‑1 (MQ=255)

                                                             tGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAAc                            <  2:5246194/93‑1 (MQ=255)

                                                              gTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACa                           >  1:9415343/1‑93 (MQ=255)

                                                              gTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACa                           <  1:7533483/93‑1 (MQ=255)

                                                               ttCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAg                          >  1:4916599/1‑93 (MQ=255)

                                                                tCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGc                         >  1:606865/1‑93 (MQ=255)

                                                                 ccACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCg                        >  1:1696624/1‑93 (MQ=255)

                                                                   aCGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGtt                      >  2:8107334/1‑93 (MQ=255)

                                                                    cGCCTGCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGttt                     <  2:2467375/93‑1 (MQ=255)

                                                                       cTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCa                  <  1:7430484/93‑1 (MQ=255)

                                                                        ttCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAg                 >  1:3139558/1‑93 (MQ=255)

                                                                        ttCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGCAATCAAGAAACTGAAACAGCGTTTGCAg                 <  1:1179429/93‑1 (MQ=255)

                                                                        ttCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGAAGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAg                 >  1:7877264/1‑93 (MQ=255)

                                                                         tCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGc                >  1:4618075/1‑93 (MQ=255)

                                                                         tCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGc                >  2:9050616/1‑93 (MQ=255)

                                                                         tCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGc                >  2:1914670/1‑93 (MQ=255)

                                                                         tCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGc                >  1:5367793/1‑93 (MQ=255)

                                                                           aaCGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCgt              >  2:2968751/1‑93 (MQ=255)

                                                                            aCGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCgtg             >  2:3654643/1‑93 (MQ=255)

                                                                              gggCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGtgtg           <  2:6505558/93‑1 (MQ=255)

                                                                              gggCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGCGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGtgtg           >  1:5226536/1‑93 (MQ=255)

                                                                                gCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGtgtgct         >  1:2629091/1‑91 (MQ=255)

                                                                                gCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGt         <  1:548291/93‑1 (MQ=255)

                                                                                 cGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTa        >  1:2139484/1‑93 (MQ=255)

                                                                                   ttAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAAc      >  1:405934/1‑93 (MQ=255)

                                                                                    tAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAAcc     >  2:3621868/1‑93 (MQ=255)

                                                                                      aCAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCAt   >  1:3896172/1‑93 (MQ=255)

                                                                                       cAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATc  >  1:2059843/1‑93 (MQ=255)

CCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATC  >  NC_000913/1261855‑1262034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCC  >  NC_000913/1261853‑1262035

CGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGC                                                                                           >  SRR4302540.6994567/1‑94 (MQ=60)

 GCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCG                                                                                          >  SRR4302540.1002689/1‑94 (MQ=60)

   CCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAA                                                                                        >  SRR4302540.6908605/1‑94 (MQ=60)

   CCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAA                                                                                        >  SRR4302540.953627/1‑94 (MQ=60)

    CGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAAT                                                                                       >  SRR4302540.3316013/1‑94 (MQ=60)

    CGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAAT                                                                                       >  SRR4302540.262215/1‑94 (MQ=60)

     GCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATA                                                                                      >  SRR4302540.1706109/1‑94 (MQ=60)

     GCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATA                                                                                      >  SRR4302540.4516961/1‑94 (MQ=60)

      CTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATAT                                                                                     >  SRR4302540.3555949/1‑94 (MQ=60)

      CTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATAT                                                                                     >  SRR4302540.3111697/1‑94 (MQ=60)

      CTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATAT                                                                                     >  SRR4302540.733682/1‑94 (MQ=60)

      CTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATAT                                                                                     >  SRR4302540.4986394/1‑94 (MQ=60)

       TGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATC                                                                                    <  SRR4302540.1252255/94‑1 (MQ=60)

       TGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATC                                                                                    >  SRR4302540.5912361/1‑94 (MQ=60)

       TGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATC                                                                                    >  SRR4302540.7760662/1‑94 (MQ=60)

       TGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATC                                                                                    <  SRR4302540.9579408/94‑1 (MQ=60)

        GTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCC                                                                                   >  SRR4302540.9326720/1‑94 (MQ=60)

        GTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCC                                                                                   >  SRR4302540.6667198/1‑94 (MQ=60)

          CTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCC                                                                                 >  SRR4302540.6334055/1‑94 (MQ=60)

           TGCCGCAGTATTCATCAACAATCGCGCTGCGCGAACGATCAGGTTCTCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCA                                                                                <  SRR4302540.3923288/94‑1 (MQ=60)

            GCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCAT                                                                               <  SRR4302540.2371122/94‑1 (MQ=60)

             CCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATC                                                                              <  SRR4302540.1102335/94‑1 (MQ=60)

              CGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCG                                                                             >  SRR4302540.9414926/1‑94 (MQ=60)

              CGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCG                                                                             >  SRR4302540.9726795/1‑94 (MQ=60)

              CGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCG                                                                             >  SRR4302540.118154/1‑94 (MQ=60)

               GCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGT                                                                            >  SRR4302540.6494537/1‑94 (MQ=60)

                CAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTC                                                                           >  SRR4302540.4518189/1‑94 (MQ=60)

                CAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTC                                                                           >  SRR4302540.3085448/1‑94 (MQ=60)

                 AGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCA                                                                          >  SRR4302540.4844035/1‑94 (MQ=60)

                 AGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCA                                                                          >  SRR4302540.4844036/1‑94 (MQ=60)

                  GTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCAC                                                                         >  SRR4302540.4318183/1‑94 (MQ=60)

                  GTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATACCCATCGTCAC                                                                         >  SRR4302540.7836125/1‑94 (MQ=60)

                  GTGTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCAC                                                                         <  SRR4302540.4554900/94‑1 (MQ=60)

                   TTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACG                                                                        <  SRR4302540.3631567/94‑1 (MQ=60)

                    TTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGA                                                                       >  SRR4302540.8963969/1‑94 (MQ=60)

                    TTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGA                                                                       >  SRR4302540.694780/1‑94 (MQ=60)

                    TTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACCGGCGTTAACAGGCGAATATCCCCATCGTCACGA                                                                       >  SRR4302540.6721811/1‑94 (MQ=60)

                    TTCCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGGTAACAGGCGAATATCCCCATCGTCACGA                                                                       >  SRR4302540.9099353/1‑94 (MQ=60)

                     TTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAA                                                                      <  SRR4302540.571207/94‑1 (MQ=60)

                      TCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAG                                                                     <  SRR4302540.2714332/94‑1 (MQ=60)

                        ATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCT                                                                   >  SRR4302540.6160315/1‑94 (MQ=60)

                        ATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCT                                                                   >  SRR4302540.3647123/1‑94 (MQ=60)

                          CAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCA                                                                 <  SRR4302540.6269168/94‑1 (MQ=60)

                          CAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCA                                                                 <  SRR4302540.7977380/94‑1 (MQ=60)

                          CAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCA                                                                 <  SRR4302540.713440/94‑1 (MQ=60)

                           AACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAA                                                                >  SRR4302540.302238/1‑94 (MQ=60)

                            ACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAAT                                                               <  SRR4302540.8602724/94‑1 (MQ=60)

                             CAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATG                                                              >  SRR4302540.5691309/1‑94 (MQ=60)

                             CAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATG                                                              <  SRR4302540.5499064/94‑1 (MQ=60)

                              AATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGC                                                             <  SRR4302540.8468943/94‑1 (MQ=60)

                              AAACGCGCTGCGCGAACGATCAGGTTATCTTCATGCTCCAAGGCTTCAACGGGGGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGC                                                             <  SRR4302540.6428106/94‑1 (MQ=60)

                               ATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCT                                                            >  SRR4302540.6549681/1‑94 (MQ=60)

                               ATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCT                                                            >  SRR4302540.2608772/1‑94 (MQ=60)

                               ATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCT                                                            <  SRR4302540.8307724/94‑1 (MQ=60)

                               ATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCT                                                            <  SRR4302540.8896556/94‑1 (MQ=60)

                               ATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCT                                                            >  SRR4302540.6549682/1‑94 (MQ=60)

                                ACGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTG                                                           <  SRR4302540.3895430/94‑1 (MQ=60)

                                 CGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGA                                                          >  SRR4302540.1847003/1‑94 (MQ=60)

                                  GCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGAT                                                         >  SRR4302540.8573116/1‑94 (MQ=60)

                                  GCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGAT                                                         <  SRR4302540.3563002/94‑1 (MQ=60)

                                   CGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATG                                                        <  SRR4302540.2903405/94‑1 (MQ=60)

                                   CGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATG                                                        >  SRR4302540.7568772/1‑94 (MQ=60)

                                    GCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGG                                                       >  SRR4302540.406110/1‑94 (MQ=60)

                                     CTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGT                                                      <  SRR4302540.3529110/94‑1 (MQ=60)

                                     CTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGT                                                      <  SRR4302540.381090/94‑1 (MQ=60)

                                     CTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGT                                                      >  SRR4302540.7900032/1‑94 (MQ=60)

                                      TGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTG                                                     >  SRR4302540.1893899/1‑94 (MQ=60)

                                      TGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTG                                                     >  SRR4302540.5506687/1‑94 (MQ=60)

                                        CGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTG                                                   >  SRR4302540.2792426/1‑94 (MQ=60)

                                         GCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCG                                                  >  SRR4302540.404519/1‑94 (MQ=60)

                                           GAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCC                                                <  SRR4302540.8262887/94‑1 (MQ=60)

                                            AACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCG                                               >  SRR4302540.3921715/1‑94 (MQ=60)

                                             ACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGT                                              >  SRR4302540.8543436/1‑94 (MQ=60)

                                              CGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTA                                             >  SRR4302540.7166228/1‑94 (MQ=60)

                                              CGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTA                                             >  SRR4302540.2320357/1‑94 (MQ=60)

                                               GATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAA                                            >  SRR4302540.7028419/1‑94 (MQ=60)

                                               GATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAA                                            >  SRR4302540.3904903/1‑94 (MQ=60)

                                               GATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAA                                            >  SRR4302540.489198/1‑94 (MQ=60)

                                               GATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAA                                            >  SRR4302540.1817338/1‑94 (MQ=60)

                                                  CAGGTTCTCTTCATGGTCCACGCCTTCAACGGGCGTTAGCAGGCGAATAGCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCA                                         <  SRR4302540.9626716/94‑1 (MQ=60)

                                                  CAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCA                                         >  SRR4302540.3548253/1‑94 (MQ=60)

                                                  CAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCA                                         >  SRR4302540.6933025/1‑94 (MQ=60)

                                                  CAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCA                                         <  SRR4302540.5540062/94‑1 (MQ=60)

                                                  CAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATAA                                         >  SRR4302540.8009974/1‑94 (MQ=60)

                                                    GGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAG                                       >  SRR4302540.822277/1‑94 (MQ=60)

                                                     GTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGA                                      >  SRR4302540.5501372/1‑94 (MQ=60)

                                                     GTTATCTTCATGTGCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGA                                      <  SRR4302540.4604121/94‑1 (MQ=60)

                                                      TTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAA                                     >  SRR4302540.5861793/1‑94 (MQ=60)

                                                        ATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAAC                                   <  SRR4302540.6326188/94‑1 (MQ=60)

                                                         TCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACT                                  >  SRR4302540.7993650/1‑94 (MQ=60)

                                                         TCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGCCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACT                                  <  SRR4302540.6518570/94‑1 (MQ=60)

                                                          CTTCATGTTCCGCGCCTTCAACGGGCGTGAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTG                                 <  SRR4302540.9765010/94‑1 (MQ=60)

                                                          CTTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTG                                 >  SRR4302540.2385814/1‑94 (MQ=60)

                                                           TTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGA                                >  SRR4302540.5419401/1‑94 (MQ=60)

                                                           TTCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGA                                >  SRR4302540.6755233/1‑94 (MQ=60)

                                                            TCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAAGTGAA                               >  SRR4302540.9553113/1‑94 (MQ=60)

                                                            TCATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAA                               >  SRR4302540.3935320/1‑94 (MQ=60)

                                                              ATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAAC                             >  SRR4302540.2106663/1‑94 (MQ=60)

                                                              ATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAAC                             >  SRR4302540.9497682/1‑94 (MQ=60)

                                                              ATGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAAC                             <  SRR4302540.2095001/94‑1 (MQ=60)

                                                               TGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACA                            >  SRR4302540.7108036/1‑94 (MQ=60)

                                                               TGTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACA                            >  SRR4302540.9527273/1‑94 (MQ=60)

                                                                GTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCACTGCTGATGGTGTCGCCGTAATCAAGCAACTGAAACAC                           >  SRR4302540.4969545/1‑94 (MQ=60)

                                                                GTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAG                           <  SRR4302540.7617846/94‑1 (MQ=60)

                                                                GTTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAG                           >  SRR4302540.4969546/1‑94 (MQ=60)

                                                                 TTCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGC                          >  SRR4302540.613353/1‑94 (MQ=60)

                                                                  TCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCG                         >  SRR4302540.1245655/1‑94 (MQ=60)

                                                                  TCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCG                         >  SRR4302540.1715005/1‑94 (MQ=60)

                                                                  TCCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCG                         <  SRR4302540.1641142/94‑1 (MQ=60)

                                                                   CCACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGT                        >  SRR4302540.3154335/1‑94 (MQ=60)

                                                                    TACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTT                       >  SRR4302540.3627987/1‑94 (MQ=60)

                                                                    CACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTT                       >  SRR4302540.7618079/1‑94 (MQ=60)

                                                                     ACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTT                      >  SRR4302540.7565898/1‑94 (MQ=60)

                                                                     ACGCCTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTT                      <  SRR4302540.6675042/94‑1 (MQ=60)

                                                                         CTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAG                  >  SRR4302540.9893437/1‑94 (MQ=60)

                                                                         CTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAG                  <  SRR4302540.7513256/94‑1 (MQ=60)

                                                                         CTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAG                  >  SRR4302540.3173795/1‑94 (MQ=60)

                                                                         CTTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGAAGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAG                  >  SRR4302540.7969270/1‑94 (MQ=60)

                                                                         CTTCAAAGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAG                  <  SRR4302540.5414640/94‑1 (MQ=60)

                                                                          TTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGC                 >  SRR4302540.5426776/1‑94 (MQ=60)

                                                                          TTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGC                 >  SRR4302540.4667595/1‑94 (MQ=60)

                                                                          TTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGC                 <  SRR4302540.9451789/94‑1 (MQ=60)

                                                                          TTCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGCAATCAAGAAACTGAAACAGCGTTTGCAGC                 <  SRR4302540.1191614/94‑1 (MQ=60)

                                                                           TCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCG                >  SRR4302540.3654433/1‑94 (MQ=60)

                                                                           TCAACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCG                <  SRR4302540.5271423/94‑1 (MQ=60)

                                                                              ACGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGT             <  SRR4302540.6949293/94‑1 (MQ=60)

                                                                               CGGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGCGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTG            >  SRR4302540.5283529/1‑94 (MQ=60)

                                                                                GGGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGG           <  SRR4302540.5670174/94‑1 (MQ=60)

                                                                                 GGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGT          >  SRR4302540.2657548/1‑94 (MQ=60)

                                                                                 GGCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGCT          >  SRR4302540.2657547/1‑94 (MQ=60)

                                                                                 GACGTTAATAGCCGAATAACACCATCGTCACGAAGCGCAGTCCTTATGGTGCCGCCGTACTCACGAAGCTGAAACAGCGTTTGCAGCGTGTGGT          <  SRR4302540.7322282/94‑1 (MQ=21)

                                                                                  GCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTA         >  SRR4302540.2162477/1‑94 (MQ=60)

                                                                                  GCGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTA         <  SRR4302540.554264/94‑1 (MQ=60)

                                                                                   CGTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAA        >  SRR4302540.1755771/1‑94 (MQ=60)

                                                                                    GTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAAC       >  SRR4302540.410176/1‑94 (MQ=60)

                                                                                    GTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAAC       >  SRR4302540.4961394/1‑94 (MQ=60)

                                                                                    GTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAAC       >  SRR4302540.1247527/1‑94 (MQ=60)

                                                                                    GTTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAAC       >  SRR4302540.888815/1‑94 (MQ=60)

                                                                                     TTAACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACC      >  SRR4302540.1971905/1‑94 (MQ=60)

                                                                                       AACAGGCGAATGTCCGCATCGTCACGAAGCTCACTGATGATGTTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCAT    <  SRR4302540.3698482/94‑1 (MQ=60)

                                                                                       AACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCAT    >  SRR4302540.3938455/1‑94 (MQ=60)

                                                                                       AACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCAT    >  SRR4302540.8218295/1‑94 (MQ=60)

                                                                                        ACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATC   >  SRR4302540.2082013/1‑94 (MQ=60)

                                                                                        ACAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATC   <  SRR4302540.7253933/94‑1 (MQ=60)

                                                                                         CAGGCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCC  <  SRR4302540.9174841/94‑1 (MQ=60)

CGCCCGCTGTCTGCCGCAGTTTTCATCAACAATCGCGCTGCGCGAACGATCAGGTTATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCC  >  NC_000913/1261853‑1262035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`