Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,249,670	A→G	V131A (GTC→GCC)	b1200 ←	putative dihydroxyacetone kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,249,670	0	A	G	100.0%	673.4 / NA	210	V131A (GTC→GCC)	b1200	putative dihydroxyacetone kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (102/108); total (102/108)

Only 100 of 215 total aligned reads displayed.

CCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATC  >  NC_000913/1249580‑1249759

ccTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGcc                                                                                         >  2:1135336/1‑93 (MQ=255)

 cTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCca                                                                                        >  1:4130536/1‑93 (MQ=255)

  tACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCCACGTCGTCCTCAATGGCcac                                                                                       >  2:3768602/1‑93 (MQ=255)

  tACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCcac                                                                                       >  2:4049239/1‑93 (MQ=255)

  tACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCcac                                                                                       <  1:2662789/93‑1 (MQ=255)

   aCGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCcaca                                                                                      <  2:1907206/93‑1 (MQ=255)

    cGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAg                                                                                     <  2:27924/93‑1 (MQ=255)

    cGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAg                                                                                     >  2:3397527/1‑93 (MQ=255)

    cGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAg                                                                                     <  1:3382906/93‑1 (MQ=255)

     gAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGt                                                                                    <  1:490903/93‑1 (MQ=255)

        tttttCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGt                                                                                 <  2:89598/93‑1 (MQ=255)

        tttttCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGt                                                                                 >  2:5100912/1‑93 (MQ=255)

          tttCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCa                                                                               <  1:1158697/93‑1 (MQ=255)

           ttCAATTAATACGGTGTTGGTAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCAc                                                                              <  2:2021617/93‑1 (MQ=255)

                ttAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTa                                                                         >  2:5678480/1‑93 (MQ=255)

                ttAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTa                                                                         <  1:3405776/93‑1 (MQ=255)

                ttAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTa                                                                         <  1:4233420/93‑1 (MQ=255)

                  aaTACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAcg                                                                       >  1:5638042/1‑93 (MQ=255)

                   aTACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAcgc                                                                      >  1:1736266/1‑93 (MQ=255)

                    tACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAcgcc                                                                     <  2:1337950/93‑1 (MQ=255)

                     aCGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAcgccg                                                                    >  1:5564284/1‑93 (MQ=255)

                     aCGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAcgccg                                                                    <  2:951031/93‑1 (MQ=255)

                      cGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAcgccgc                                                                   <  2:4674314/93‑1 (MQ=255)

                       ggTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCt                                                                  >  2:5829107/1‑93 (MQ=255)

                       ggTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCt                                                                  <  2:3322209/93‑1 (MQ=255)

                        gtgtTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTa                                                                 >  1:3621485/1‑93 (MQ=255)

                         tgtTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTAt                                                                <  1:86117/93‑1 (MQ=255)

                         tgtTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTAt                                                                <  2:3451745/93‑1 (MQ=255)

                           ttGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCg                                                              <  2:5778535/93‑1 (MQ=255)

                           ttGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCg                                                              >  2:6057309/1‑93 (MQ=255)

                           ttGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCg                                                              <  1:2208890/93‑1 (MQ=255)

                            tGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGt                                                             <  1:4779012/93‑1 (MQ=255)

                            tGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGt                                                             >  1:38644/1‑93 (MQ=255)

                            tGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGt                                                             <  1:5775951/93‑1 (MQ=255)

                             ggCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCgca                                                            <  1:2116659/93‑3 (MQ=255)

                              gCAACGCCGCGTCGCCTGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCCCTTTTACGCCGCTATCGTGc                                                           <  1:5167703/93‑1 (MQ=255)

                              gCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGc                                                           >  2:3178003/1‑93 (MQ=255)

                              gCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGc                                                           >  2:1578173/1‑93 (MQ=255)

                               cAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCa                                                          >  2:3342791/1‑93 (MQ=255)

                                 aCGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGt                                                        >  1:1430970/1‑93 (MQ=255)

                                 aCGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGt                                                        >  1:2318553/1‑93 (MQ=255)

                                  cgccgcGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTa                                                       <  1:4194068/93‑1 (MQ=255)

                                   gccgcGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTaa                                                      <  1:3656307/93‑1 (MQ=255)

                                    ccgcGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAAc                                                     >  2:5223838/1‑93 (MQ=255)

                                    ccgcGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAAc                                                     >  1:4035530/1‑93 (MQ=255)

                                     cgcgTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACt                                                    <  1:6287654/93‑1 (MQ=255)

                                      gcgTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTc                                                   >  2:4838982/1‑93 (MQ=255)

                                      gcgTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTc                                                   >  2:2344432/1‑93 (MQ=255)

                                      gcgTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTc                                                   >  2:2136571/1‑93 (MQ=255)

                                       cgTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCg                                                  <  2:1638024/93‑1 (MQ=255)

                                        gTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCgg                                                 >  1:3556401/1‑93 (MQ=255)

                                        gTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCgg                                                 >  2:1434006/1‑93 (MQ=255)

                                        gTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCgg                                                 <  2:3155597/93‑1 (MQ=255)

                                         tCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGt                                                <  2:879356/93‑1 (MQ=255)

                                          cccccGGTAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTc                                               <  2:1693764/91‑1 (MQ=255)

                                          cGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTc                                               <  2:654641/93‑1 (MQ=255)

                                             ccGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCt                                            >  1:553518/1‑93 (MQ=255)

                                               ggCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGt                                          <  2:5571169/93‑1 (MQ=255)

                                                   gTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCa                                      <  2:2214417/93‑1 (MQ=255)

                                                   gTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCa                                      >  2:5367863/1‑93 (MQ=255)

                                                    tataAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCaa                                     <  1:2968509/93‑1 (MQ=255)

                                                    tataAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCaa                                     <  1:2570198/93‑1 (MQ=255)

                                                     ataAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCaaa                                    >  1:6107766/1‑93 (MQ=255)

                                                      taAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAg                                   >  2:2235971/1‑93 (MQ=255)

                                                      taAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAg                                   >  2:387750/1‑93 (MQ=255)

                                                       aaaGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGt                                  >  2:4819842/1‑93 (MQ=255)

                                                       aaaGACTGTCTTTTACCGCAACGTCGTCATCAATAGCCACAGTGGTCACTTTTACGCCTCTATCGTGCAGTAACTCGGTCGCTGTTTCACAGt                                  >  1:4556747/1‑93 (MQ=255)

                                                         aGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTa                                >  2:2567357/1‑93 (MQ=255)

                                                          gACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTaa                               <  2:4119909/93‑1 (MQ=255)

                                                          gACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTaa                               <  2:2082203/93‑1 (MQ=255)

                                                          gACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTaa                               <  2:5012809/93‑1 (MQ=255)

                                                           aCTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAg                              <  2:1220485/93‑1 (MQ=255)

                                                            cTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGa                             <  1:3229424/93‑1 (MQ=255)

                                                             tGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGaa                            <  2:1068817/93‑1 (MQ=255)

                                                              gTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAat                           >  2:2372205/1‑93 (MQ=255)

                                                                cTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAatat                         >  2:2880357/1‑93 (MQ=255)

                                                                cTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAatat                         <  2:1492218/93‑1 (MQ=255)

                                                                    tACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGcc                     <  1:2130053/93‑1 (MQ=255)

                                                                     aCCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCg                    >  1:2606998/1‑93 (MQ=255)

                                                                      ccGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCgg                   >  2:2502760/1‑93 (MQ=255)

                                                                        gCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGgtg                 >  2:4605149/1‑93 (MQ=255)

                                                                           aCGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTaa              <  1:5096357/93‑1 (MQ=255)

                                                                            cgtcgtCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAt             <  1:5808228/93‑1 (MQ=255)

                                                                             gtcgtcATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAtt            <  1:274326/93‑1 (MQ=255)

                                                                              tcgtcATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAttt           <  2:56188/93‑1 (MQ=255)

                                                                              tcgtcATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAttt           >  2:3518073/1‑93 (MQ=255)

                                                                               cgtcATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAtttt          <  2:6208471/93‑1 (MQ=255)

                                                                                gtcATCAATGGCCACAGTGGTCGCTTTTACGCCGCTATCGTGCAGTATCTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAttttt         <  2:1659688/93‑1 (MQ=255)

                                                                                gtcATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAttttt         >  1:823843/1‑93 (MQ=255)

                                                                                gtcATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAttttt         >  2:2618187/1‑93 (MQ=255)

                                                                                  catcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGa       <  1:2339920/93‑1 (MQ=255)

                                                                                   atcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGAt      <  1:451663/93‑1 (MQ=255)

                                                                                   atcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGAt      >  1:1794006/1‑93 (MQ=255)

                                                                                    tcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATa     <  2:700480/93‑1 (MQ=255)

                                                                                    tcaATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATa     >  2:2094793/1‑93 (MQ=255)

                                                                                     caATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATaa    >  1:4323399/1‑93 (MQ=255)

                                                                                       aTGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATc  >  2:4423043/1‑93 (MQ=255)

                                                                                       aTGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATc  <  2:3946495/93‑1 (MQ=255)

                                                                                       aTGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATc  >  1:3936354/1‑93 (MQ=255)

                                                                                       aTGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATc  >  1:6018098/1‑93 (MQ=255)

CCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATC  >  NC_000913/1249580‑1249759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACA  >  NC_000913/1249577‑1249763

GCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGG                                                                                               <  SRR4302533.456479/94‑1 (MQ=60)

 CGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGC                                                                                              >  SRR4302533.2044853/1‑94 (MQ=60)

   CCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCA                                                                                            >  SRR4302533.4155607/1‑94 (MQ=60)

     TACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACA                                                                                          <  SRR4302533.2679136/94‑1 (MQ=60)

       CGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGT                                                                                        <  SRR4302533.3403789/94‑1 (MQ=60)

       CGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGT                                                                                        <  SRR4302533.6162414/94‑1 (MQ=60)

        GAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTG                                                                                       <  SRR4302533.493837/94‑1 (MQ=60)

         AGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGG                                                                                      >  SRR4302533.4528622/1‑94 (MQ=60)

          GTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGT                                                                                     >  SRR4302533.6302464/1‑94 (MQ=60)

             TTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCAC                                                                                  <  SRR4302533.1165651/94‑1 (MQ=60)

                 AATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTT                                                                              >  SRR4302533.365794/1‑94 (MQ=60)

                  ATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTA                                                                             >  SRR4302533.2433842/1‑94 (MQ=60)

                   TTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAC                                                                            <  SRR4302533.3426812/94‑1 (MQ=60)

                   TTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTAC                                                                            <  SRR4302533.4259002/94‑1 (MQ=60)

                    TAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACG                                                                           >  SRR4302533.5672131/1‑94 (MQ=60)

                     AATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGC                                                                          >  SRR4302533.1746802/1‑94 (MQ=60)

                       TACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCG                                                                        <  SRR4302533.3610598/94‑1 (MQ=60)

                       TACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCG                                                                        >  SRR4302533.5597916/1‑94 (MQ=60)

                         CGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCT                                                                      >  SRR4302533.6326848/1‑94 (MQ=60)

                         CGGTGTCGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCT                                                                      <  SRR4302533.1316795/94‑1 (MQ=60)

                          GGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTA                                                                     >  SRR4302533.3643770/1‑94 (MQ=60)

                           GTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTAT                                                                    >  SRR4302533.2081208/1‑94 (MQ=60)

                            TGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATC                                                                   <  SRR4302533.86670/94‑1 (MQ=60)

                            TGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATC                                                                   <  SRR4302533.5003122/94‑1 (MQ=60)

                              TTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGT                                                                 <  SRR4302533.2222513/94‑1 (MQ=60)

                              TTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGT                                                                 >  SRR4302533.38899/1‑94 (MQ=60)

                               TGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTG                                                                <  SRR4302533.4807959/94‑1 (MQ=60)

                               TGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTG                                                                <  SRR4302533.5810833/94‑1 (MQ=60)

                               TGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTG                                                                >  SRR4302533.4335997/1‑94 (MQ=60)

                                GGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGcat                                                               <  SRR4302533.2129715/94‑4 (MQ=60)

                                 GCAACGCCGCGTCGCCTGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCCCTTTTACGCCGCTATCGTGCA                                                              <  SRR4302533.5198899/94‑1 (MQ=60)

                                  CAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAG                                                             >  SRR4302533.5336964/1‑94 (MQ=60)

                                  CAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAG                                                             >  SRR4302533.5127898/1‑94 (MQ=60)

                                  CAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATAAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAG                                                             >  SRR4302533.1645075/1‑94 (MQ=60)

                                   AACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGT                                                            >  SRR4302533.2332907/1‑94 (MQ=60)

                                   AACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGT                                                            >  SRR4302533.1439619/1‑94 (MQ=60)

                                   AACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGT                                                            >  SRR4302533.3897606/1‑94 (MQ=60)

                                     CGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAA                                                          <  SRR4302533.4219451/94‑1 (MQ=60)

                                      GCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAAC                                                         >  SRR4302533.4060095/1‑94 (MQ=60)

                                      GCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAAC                                                         <  SRR4302533.3678781/94‑1 (MQ=60)

                                        CGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTC                                                       <  SRR4302533.1797924/94‑1 (MQ=60)

                                        CGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTC                                                       <  SRR4302533.6325164/94‑1 (MQ=60)

                                         GCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCG                                                      >  SRR4302533.5586144/1‑94 (MQ=60)

                                         GCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCG                                                      <  SRR4302533.4005248/94‑1 (MQ=60)

                                          CGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGG                                                     <  SRR4302533.3500435/94‑1 (MQ=60)

                                          CGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGG                                                     >  SRR4302533.3578319/1‑94 (MQ=60)

                                            TCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTC                                                   <  SRR4302533.4237016/94‑1 (MQ=60)

                                            GCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTC                                                   <  SRR4302533.4536595/94‑1 (MQ=60)

                                               CCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCT                                                >  SRR4302533.556799/1‑94 (MQ=60)

                                                CCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTG                                               >  SRR4302533.92509/1‑94 (MQ=60)

                                                     AGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCA                                          <  SRR4302533.2324669/94‑1 (MQ=60)

                                                       TATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAA                                        <  SRR4302533.2586015/94‑1 (MQ=60)

                                                       TATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAA                                        <  SRR4302533.2986660/94‑1 (MQ=60)

                                                       TATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAA                                        >  SRR4302533.2323022/1‑94 (MQ=60)

                                                       TATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAA                                        >  SRR4302533.6144641/1‑94 (MQ=60)

                                                       TATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAA                                        >  SRR4302533.6025686/1‑94 (MQ=60)

                                                        ATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAG                                       <  SRR4302533.4875440/94‑1 (MQ=60)

                                                        ATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAG                                       <  SRR4302533.213453/94‑1 (MQ=60)

                                                         TAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGT                                      <  SRR4302533.2720543/94‑1 (MQ=60)

                                                         TAAAGACTGTCTTTTACCGCAACGTCGTCATCAATAGCCACAGTGGTCACTTTTACGCCTCTATCGTGCAGTAACTCGGTCGCTGTTTCACAGT                                      >  SRR4302533.4584381/1‑94 (MQ=60)

                                                          AAAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTT                                     <  SRR4302533.3363451/94‑1 (MQ=60)

                                                           AAGACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTA                                    >  SRR4302533.4185702/1‑94 (MQ=60)

                                                             GACTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAG                                  <  SRR4302533.5594383/94‑1 (MQ=60)

                                                               CTGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAA                                <  SRR4302533.3249334/94‑1 (MQ=60)

                                                                TGTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAAT                               >  SRR4302533.261711/1‑94 (MQ=60)

                                                                 GTCTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATA                              >  SRR4302533.2914513/1‑94 (MQ=60)

                                                                   CTTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATC                            <  SRR4302533.4556007/94‑1 (MQ=60)

                                                                    TTTTACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCG                           <  SRR4302533.2336598/94‑1 (MQ=60)

                                                                       TACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCG                        <  SRR4302533.2143187/94‑1 (MQ=60)

                                                                       TACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCG                        >  SRR4302533.2664265/1‑94 (MQ=60)

                                                                       TACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCG                        >  SRR4302533.2623010/1‑94 (MQ=60)

                                                                        ACCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGG                       <  SRR4302533.3008020/94‑1 (MQ=60)

                                                                         CCGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGT                      >  SRR4302533.2169660/1‑94 (MQ=60)

                                                                          CGCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTG                     <  SRR4302533.5843056/94‑1 (MQ=60)

                                                                           GCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGT                    <  SRR4302533.3758498/94‑1 (MQ=60)

                                                                           GCAACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGT                    <  SRR4302533.5638763/94‑1 (MQ=60)

                                                                              ACGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAAT                 <  SRR4302533.5127150/94‑1 (MQ=60)

                                                                               CGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATT                <  SRR4302533.4091012/94‑1 (MQ=60)

                                                                               CGTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATT                <  SRR4302533.5843302/94‑1 (MQ=60)

                                                                                GTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTT               <  SRR4302533.275995/94‑1 (MQ=60)

                                                                                GTCGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTT               >  SRR4302533.5702336/1‑94 (MQ=60)

                                                                                 TCGTCATCAATGGCCACAGTGTTCTCTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTT              <  SRR4302533.3410602/94‑1 (MQ=60)

                                                                                  CGTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTT             >  SRR4302533.828677/1‑94 (MQ=60)

                                                                                   GTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTG            <  SRR4302533.4726521/94‑1 (MQ=60)

                                                                                   GTCATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTG            <  SRR4302533.6066501/94‑1 (MQ=60)

                                                                                     CATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGAT          <  SRR4302533.2354410/94‑1 (MQ=60)

                                                                                     CATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGAT          >  SRR4302533.1804922/1‑94 (MQ=60)

                                                                                      ATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATA         >  SRR4302533.372880/1‑94 (MQ=60)

                                                                                      ATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATA         <  SRR4302533.829329/94‑1 (MQ=60)

                                                                                      ATCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATA         <  SRR4302533.454366/94‑1 (MQ=60)

                                                                                       TCAATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAA        >  SRR4302533.4349435/1‑94 (MQ=60)

                                                                                         AATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATC      >  SRR4302533.3960384/1‑94 (MQ=60)

                                                                                         AATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATC      <  SRR4302533.323584/94‑1 (MQ=60)

                                                                                         AATGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATC      >  SRR4302533.6054566/1‑94 (MQ=60)

                                                                                           TGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAA    <  SRR4302533.2876529/94‑1 (MQ=60)

                                                                                           TGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAA    <  SRR4302533.2478019/94‑1 (MQ=60)

                                                                                           TGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAA    <  SRR4302533.449278/94‑1 (MQ=60)

                                                                                           TGGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAA    <  SRR4302533.4306048/94‑1 (MQ=60)

                                                                                            GGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAAC   >  SRR4302533.6328621/1‑94 (MQ=60)

                                                                                            GGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAAC   <  SRR4302533.2857019/94‑1 (MQ=60)

                                                                                            GGCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAAC   <  SRR4302533.3001603/94‑1 (MQ=60)

                                                                                             GCCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACA  <  SRR4302533.4092102/94‑1 (MQ=60)

                                                                                             GCCACAGTGGTCACTCTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAATTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACA  <  SRR4302533.6308313/94‑1 (MQ=60)

GCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACA  >  NC_000913/1249577‑1249763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`