Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,263,021 G→A D29N (GAT→AAT)  hemA → HemA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,263,0210GA99.6% 925.7 / ‑5.4 254D29N (GAT→AAT) hemAHemA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (140/112);  total (141/113)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

Only 100 of 266 total aligned reads displayed.

GCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC  >  NC_000913/1262931‑1263110
                                                                                          |                                                                                         
 cAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATc                                                                                        >  1:8490530/1‑93 (MQ=255)
  aggcATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCa                                                                                       <  2:3036057/90‑1 (MQ=255)
   ggcATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAg                                                                                      >  2:3795109/3‑93 (MQ=255)
   ggcATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAg                                                                                      >  1:2095550/3‑93 (MQ=255)
   gACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAg                                                                                      >  1:7920584/1‑93 (MQ=255)
    gcATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAgg                                                                                     <  2:1500086/92‑1 (MQ=255)
    gcATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAgg                                                                                     >  1:1569329/2‑93 (MQ=255)
    gcATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAgg                                                                                     <  2:3097346/92‑1 (MQ=255)
     cATGACCCTTTTAGCACTCGGTATCAACCGTAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGgc                                                                                    >  1:4127951/1‑93 (MQ=255)
     cATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGgc                                                                                    <  1:6604758/93‑1 (MQ=255)
     cATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGgc                                                                                    <  1:3186078/93‑1 (MQ=255)
     cATGACACTTTTAGCACTCGGTATCAACCATAAAACGGCAACTGTATCGCTGCGAGAACGTGTGTCGTTTTCGACTGATAAGATTAATCAgac                                                                                    >  2:8236642/1‑91 (MQ=255)
       tGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGgcgc                                                                                  >  1:6435395/1‑93 (MQ=255)
        gACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCt                                                                                 <  1:6131351/93‑1 (MQ=255)
         aCCCTTTTGGCACGCGGGGTGATCCATAAAACTGCACCTGTATCGCTGTGAGGACGTGTATGGTTTTCGCCGGATAATCTCAATAAGgtgcgt                                                                                >  2:4822139/1‑91 (MQ=255)
         aCCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCtt                                                                                <  1:3329883/93‑1 (MQ=255)
          cccTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTg                                                                               >  1:1907865/1‑93 (MQ=255)
           ccTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTACCGTTTTCGCCTGATAAGCTCAATCAGGCGCTTGa                                                                              >  2:6861823/1‑93 (MQ=255)
            cTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGAc                                                                             <  1:6868245/93‑1 (MQ=255)
            cTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGAc                                                                             >  2:4476028/1‑93 (MQ=255)
            cTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGAc                                                                             <  1:1260968/93‑1 (MQ=255)
            cTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGAc                                                                             <  1:9769975/93‑1 (MQ=255)
              tttAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAg                                                                           <  2:80144/93‑1 (MQ=255)
                 tgCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCt                                                                        <  2:7714736/92‑1 (MQ=255)
                 aGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACCGCCt                                                                        >  2:2221141/1‑93 (MQ=255)
                 aGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCt                                                                        >  1:2627431/1‑93 (MQ=255)
                 aGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCt                                                                        <  1:6757815/93‑1 (MQ=255)
                 aGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCt                                                                        >  1:8146971/1‑93 (MQ=255)
                  gCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTg                                                                       >  1:2705988/1‑93 (MQ=255)
                  gCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTg                                                                       <  1:4980129/93‑1 (MQ=255)
                   cACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGc                                                                      <  2:8178191/93‑1 (MQ=255)
                    ccTCGGTATCAACCATAAAACGGCACCTGTATGGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCt                                                                     <  1:5978283/92‑1 (MQ=255)
                    aCTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCt                                                                     <  2:2452374/93‑1 (MQ=255)
                    aCTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCt                                                                     <  2:7966426/93‑1 (MQ=255)
                     cTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGATCAATCAGGCGCTTGACAGCCTGCtt                                                                    >  1:2381448/1‑93 (MQ=255)
                      tCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTg                                                                   <  2:5989549/93‑1 (MQ=255)
                       cGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCGGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTgc                                                                  >  2:2118596/1‑93 (MQ=255)
                        ggTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTgcg                                                                 <  1:5617622/93‑1 (MQ=255)
                         gTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTgcgc                                                                >  1:7028632/1‑93 (MQ=255)
                          tATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCa                                                               >  1:9752757/1‑93 (MQ=255)
                            tCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGc                                                             >  1:1153099/1‑93 (MQ=255)
                            tCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGc                                                             <  1:7176477/93‑1 (MQ=255)
                            tCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGCATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGc                                                             >  1:4211543/1‑93 (MQ=255)
                             cAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGcc                                                            <  2:6447904/93‑1 (MQ=255)
                              aaCCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCg                                                           >  2:2105420/1‑93 (MQ=255)
                                 cATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATg                                                        >  1:7568946/1‑93 (MQ=255)
                                  aTAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATgg                                                       >  1:1392870/1‑93 (MQ=255)
                                    aaaaCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTg                                                     <  2:716508/93‑1 (MQ=255)
                                    aaaaCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTg                                                     >  2:9328021/1‑93 (MQ=255)
                                     aaaCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGc                                                    <  2:7505134/93‑1 (MQ=255)
                                      aaCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCACTCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCa                                                   >  2:6270151/1‑93 (MQ=255)
                                       aCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAg                                                  <  1:9301941/93‑1 (MQ=255)
                                       aCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCATGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAg                                                  <  2:5770022/93‑1 (MQ=255)
                                       aCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAg                                                  <  2:5668571/93‑1 (MQ=255)
                                        cGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAgg                                                 >  1:7516242/1‑93 (MQ=255)
                                        cGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTGGACAGCCTGCTTGCGCAGCCGATGGTGCAgg                                                 <  2:1784222/93‑1 (MQ=255)
                                            aCCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGggcgg                                             >  2:6199668/1‑93 (MQ=255)
                                             ccTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGggcggc                                            >  1:2068917/1‑93 (MQ=255)
                                                gTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCgtg                                         >  1:3926875/1‑93 (MQ=255)
                                                    cGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGc                                     <  1:10013485/93‑1 (MQ=255)
                                                     gCTGCGAGAACGTGTATCGTTTTCGCCCGATAAGCTCTATCAGACGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCt                                    >  2:1801885/1‑93 (MQ=255)
                                                      cTGCGAGAACGTTTATCGTTTTCGCAGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTg                                   <  2:5196901/93‑1 (MQ=255)
                                                       tGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGt                                  <  1:4601049/93‑1 (MQ=255)
                                                          gagaACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGa                               >  2:2960338/1‑93 (MQ=255)
                                                            gaACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACg                             >  2:9138160/1‑93 (MQ=255)
                                                              aagtgtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTg                           <  2:6096552/91‑1 (MQ=255)
                                                              aCGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTg                           >  2:7345930/1‑93 (MQ=255)
                                                               cGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGc                          <  2:9346015/93‑1 (MQ=255)
                                                                 tgtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCac                        >  1:3427702/1‑92 (MQ=255)
                                                                 tgtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCaa                        >  1:381853/1‑93 (MQ=255)
                                                                 tgtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCaa                        >  2:5464294/1‑93 (MQ=255)
                                                                 tgtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGAGGTGCTGTCGACGTGCaa                        >  1:9090869/1‑93 (MQ=255)
                                                                  gtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAAc                       >  2:7298151/1‑93 (MQ=255)
                                                                  gtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAAc                       <  1:4817478/93‑1 (MQ=255)
                                                                   tATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCa                                                   <  2:2618144/64‑1 (MQ=255)
                                                                   tATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAAcc                      >  1:5412171/1‑93 (MQ=255)
                                                                   tATCGTTTTCGCCGGATAAGCTCAATCAGGCCCTTGCCAGCCTGCTTGCGCAGCCGATGGTGCAGCACGGCCTGGTGCTCTCGACGTGCAccc                      >  1:7176393/1‑93 (MQ=255)
                                                                     tCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCCACCGc                    >  1:5282099/1‑93 (MQ=255)
                                                                      cGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCa                   >  2:6951604/1‑93 (MQ=255)
                                                                       gTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCAc                  <  2:2681089/93‑1 (MQ=255)
                                                                        ttttCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACg                 >  1:1943333/1‑93 (MQ=255)
                                                                        ttttCGCCGGATAAGCTCAATCAGGCGCGTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACg                 >  1:5293625/1‑93 (MQ=255)
                                                                             gCCGGATAGGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACt            <  2:8982978/93‑1 (MQ=255)
                                                                             gCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACt            >  1:3627166/1‑93 (MQ=255)
                                                                              ccGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACtt           <  2:1232260/93‑1 (MQ=255)
                                                                              ccGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTAGACGTGCAACCGCACGGAACtt           >  1:6335764/1‑93 (MQ=255)
                                                                               cGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGGGCttt          >  1:2656074/1‑93 (MQ=255)
                                                                                ggATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTa         >  1:767762/1‑93 (MQ=255)
                                                                                ggATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTa         <  2:4129331/93‑1 (MQ=255)
                                                                                ggATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTa         <  2:1737002/93‑1 (MQ=255)
                                                                                 gATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTAt        >  2:7626388/1‑93 (MQ=255)
                                                                                 gATAAGCTCAAGCAGGCGCTTGACACCCTTCTTGCCCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTAt        <  1:9563569/93‑1 (MQ=255)
                                                                                  aTAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATc       >  2:3726932/1‑93 (MQ=255)
                                                                                   tAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCt      >  2:8385229/1‑93 (MQ=255)
                                                                                   tAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCt      >  2:1170186/1‑93 (MQ=255)
                                                                                   tAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCt      >  2:956729/1‑93 (MQ=255)
                                                                                    aaGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCtt     >  1:4322861/1‑93 (MQ=255)
                                                                                      gCTCAATCAGGCGCTTGACAGGCTGCTTGCGCAGTCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAg   <  1:6885911/93‑1 (MQ=255)
                                                                                       cTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGc  <  2:6323549/93‑1 (MQ=255)
                                                                                       cTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGc  >  1:8671494/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC  >  NC_000913/1262931‑1263110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTG  >  NC_000913/1262928‑1263114
                                                                                             |                                                                                             
CCCGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCA                                                                                               >  SRR4302540.3668872/1‑94 (MQ=60)
 CCGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAA                                                                                              >  SRR4302540.895908/1‑94 (MQ=60)
  CGCAGGCTTGACCCTGTTAGCACTCGGTATCAACCAGAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAAT                                                                                             <  SRR4302540.2403251/94‑1 (MQ=60)
  CGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCAGGATAAGCTCAAT                                                                                             <  SRR4302540.8707515/94‑1 (MQ=60)
   GCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATC                                                                                            <  SRR4302540.195615/94‑1 (MQ=60)
   GCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATC                                                                                            >  SRR4302540.6977780/1‑94 (MQ=60)
   GCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATC                                                                                            <  SRR4302540.4684216/94‑1 (MQ=60)
   GCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATC                                                                                            >  SRR4302540.8589183/1‑94 (MQ=60)
     AGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAG                                                                                          >  SRR4302540.2118049/1‑94 (MQ=60)
     AGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAG                                                                                          >  SRR4302540.8012900/1‑94 (MQ=60)
      GGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGG                                                                                         >  SRR4302540.1586533/1‑94 (MQ=60)
      GGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGG                                                                                         <  SRR4302540.7277486/94‑1 (MQ=60)
      GACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGG                                                                                         <  SRR4302540.1523267/94‑1 (MQ=60)
       ACATGACCCTTTTAGCACTCGGTATCAACCGTAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGC                                                                                        >  SRR4302540.4172563/1‑94 (MQ=60)
        CATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCG                                                                                       <  SRR4302540.6677891/94‑1 (MQ=60)
        CATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCG                                                                                       <  SRR4302540.3220697/94‑1 (MQ=60)
         ATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGC                                                                                      >  SRR4302540.5828819/1‑94 (MQ=60)
         ATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGC                                                                                      >  SRR4302540.6507027/1‑94 (MQ=60)
          TGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATGGTTTTCGCCGGATAAGCTCAATCAGGCGCT                                                                                     >  SRR4302540.53366/1‑94 (MQ=60)
           GACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTT                                                                                    <  SRR4302540.6199644/94‑1 (MQ=60)
            ACCCTTTTAGCACTCGGTATCAACCATAAACCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTG                                                                                   <  SRR4302540.4034365/94‑1 (MQ=60)
            ACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTG                                                                                   >  SRR4302540.1928713/1‑94 (MQ=60)
            ACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTG                                                                                   <  SRR4302540.3366437/94‑1 (MQ=60)
              CCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGAC                                                                                 <  SRR4302540.6047364/94‑1 (MQ=60)
               CTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACA                                                                                <  SRR4302540.6944383/94‑1 (MQ=60)
               CTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACA                                                                                >  SRR4302540.6397593/1‑94 (MQ=60)
               CTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACA                                                                                <  SRR4302540.9884529/94‑1 (MQ=60)
               CTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACA                                                                                >  SRR4302540.2668606/1‑94 (MQ=60)
               CTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACA                                                                                <  SRR4302540.1273902/94‑1 (MQ=60)
                   TAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCT                                                                            >  SRR4302540.2655857/1‑94 (MQ=60)
                   TAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCT                                                                            >  SRR4302540.8241547/1‑94 (MQ=60)
                    AGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTG                                                                           <  SRR4302540.6832496/94‑1 (MQ=60)
                    AGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTG                                                                           >  SRR4302540.2735174/1‑94 (MQ=60)
                     GCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGC                                                                          >  SRR4302540.6305523/1‑94 (MQ=60)
                     GCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGC                                                                          <  SRR4302540.5034038/94‑1 (MQ=60)
                      CACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCT                                                                         <  SRR4302540.1172458/94‑1 (MQ=60)
                       CCTCGGTATCAACCATAAAACGGCACCTGTATGGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTT                                                                        <  SRR4302540.6044464/94‑1 (MQ=60)
                       ACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTT                                                                        >  SRR4302540.3399966/1‑94 (MQ=60)
                       ACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGATCAATCAGGCGCTTGACAGCCTGCTT                                                                        >  SRR4302540.2407106/1‑94 (MQ=60)
                        CTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTG                                                                       >  SRR4302540.8499254/1‑94 (MQ=60)
                        CTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTG                                                                       >  SRR4302540.9866355/1‑94 (MQ=60)
                          CGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCG                                                                     <  SRR4302540.3602929/94‑1 (MQ=60)
                           GGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGC                                                                    >  SRR4302540.8167867/1‑94 (MQ=60)
                           GGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGC                                                                    >  SRR4302540.7106727/1‑94 (MQ=60)
                           GGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGC                                                                    <  SRR4302540.2294227/94‑1 (MQ=60)
                           GGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGC                                                                    >  SRR4302540.6943791/1‑94 (MQ=60)
                           GGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGC                                                                    <  SRR4302540.5679436/94‑1 (MQ=60)
                            GTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCA                                                                   >  SRR4302540.4621233/1‑94 (MQ=60)
                            GTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCA                                                                   >  SRR4302540.3706014/1‑94 (MQ=60)
                            GTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCA                                                                   >  SRR4302540.9867192/1‑94 (MQ=60)
                            GTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCA                                                                   >  SRR4302540.6991720/1‑94 (MQ=60)
                            GTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCA                                                                   >  SRR4302540.9867189/1‑94 (MQ=60)
                              ATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGC                                                                 >  SRR4302540.1165105/1‑94 (MQ=60)
                              ATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGC                                                                 <  SRR4302540.127747/94‑1 (MQ=60)
                              ATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGCATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGC                                                                 >  SRR4302540.4257400/1‑94 (MQ=60)
                              ATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGCATCCTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAAC                                                                 >  SRR4302540.4257399/1‑94 (MQ=60)
                               TCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCC                                                                >  SRR4302540.5590965/1‑94 (MQ=60)
                               TCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCC                                                                <  SRR4302540.7256481/94‑1 (MQ=60)
                                CAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCG                                                               >  SRR4302540.3923213/1‑94 (MQ=60)
                                  ACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGAT                                                             <  SRR4302540.3352877/94‑1 (MQ=60)
                                   CCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATG                                                            <  SRR4302540.5356777/94‑1 (MQ=60)
                                   CCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATG                                                            >  SRR4302540.7653852/1‑94 (MQ=60)
                                   CCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTGTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCCCAGCCGATG                                                            >  SRR4302540.4730615/1‑94 (MQ=60)
                                    CATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGG                                                           >  SRR4302540.1407790/1‑94 (MQ=60)
                                     ATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGT                                                          >  SRR4302540.5115535/1‑94 (MQ=60)
                                      TAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTG                                                         <  SRR4302540.7117923/94‑1 (MQ=60)
                                        AAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCA                                                       >  SRR4302540.6812571/1‑94 (MQ=60)
                                        AAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCA                                                       <  SRR4302540.7138669/94‑1 (MQ=60)
                                        AAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCA                                                       <  SRR4302540.1985094/94‑1 (MQ=60)
                                        AAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCA                                                       >  SRR4302540.8761755/1‑94 (MQ=60)
                                         AACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAG                                                      <  SRR4302540.8057664/94‑1 (MQ=60)
                                          ACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGG                                                     <  SRR4302540.9413048/94‑1 (MQ=60)
                                          ACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGG                                                     <  SRR4302540.6812698/94‑1 (MQ=60)
                                          ACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGG                                                     >  SRR4302540.7600280/1‑94 (MQ=60)
                                           CGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGG                                                    <  SRR4302540.4002382/94‑1 (MQ=60)
                                            GTCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTAAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGC                                                   <  SRR4302540.4648270/94‑1 (MQ=60)
                                            GGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGC                                                   <  SRR4302540.435543/94‑1 (MQ=60)
                                               ACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGC                                                >  SRR4302540.2091171/1‑94 (MQ=60)
                                                CCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCG                                               >  SRR4302540.9493110/1‑94 (MQ=60)
                                                CCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCG                                               >  SRR4302540.5153653/1‑94 (MQ=60)
                                                CCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCG                                               >  SRR4302540.6512007/1‑94 (MQ=60)
                                                 CTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGT                                              >  SRR4302540.5927271/1‑94 (MQ=60)
                                                  TGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTG                                             >  SRR4302540.3969354/1‑94 (MQ=60)
                                                  TGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTG                                             >  SRR4302540.4994961/1‑94 (MQ=60)
                                                   GTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGG                                            <  SRR4302540.2730597/94‑1 (MQ=60)
                                                   GTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGG                                            <  SRR4302540.6470589/94‑1 (MQ=60)
                                                   GTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGG                                            >  SRR4302540.3247482/1‑94 (MQ=60)
                                                    TATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGT                                           >  SRR4302540.9607875/1‑94 (MQ=60)
                                                     ATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGTTTGCGCAGCCGATGGTGCAGGGCTGCGTGGTG                                          <  SRR4302540.7291007/94‑1 (MQ=60)
                                                     ATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTG                                          <  SRR4302540.1614228/94‑1 (MQ=60)
                                                       CGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCT                                        >  SRR4302540.5548166/1‑94 (MQ=60)
                                                       CGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCT                                        <  SRR4302540.10130067/94‑1 (MQ=60)
                                                        GCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTG                                       <  SRR4302540.9391402/94‑1 (MQ=60)
                                                        GCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTG                                       >  SRR4302540.8908048/1‑94 (MQ=60)
                                                          TGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTC                                     <  SRR4302540.4650496/94‑1 (MQ=60)
                                                             GAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGAC                                  >  SRR4302540.1559189/1‑94 (MQ=60)
                                                             GAGAACGTGTATCGTTTGCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGAC                                  <  SRR4302540.2520588/94‑1 (MQ=60)
                                                             GAGAACGGGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCAGTCGAC                                  >  SRR4302540.2055250/1‑94 (MQ=60)
                                                               GAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGG                                >  SRR4302540.2105954/1‑94 (MQ=60)
                                                                AACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTG                               >  SRR4302540.3016464/1‑94 (MQ=60)
                                                                  CGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCA                             >  SRR4302540.3181728/1‑94 (MQ=60)
                                                                   GTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAC                            >  SRR4302540.3464954/1‑94 (MQ=60)
                                                                   GTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAA                            >  SRR4302540.385802/1‑94 (MQ=60)
                                                                   GTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGAGGTGCTGTCGACGTGCAA                            >  SRR4302540.9200086/1‑94 (MQ=60)
                                                                     GTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGaagcgctagatcggaagagcggttcagc                          >  SRR4302540.2646442/1‑66 (MQ=60)
                                                                     GTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACC                          >  SRR4302540.7256396/1‑94 (MQ=60)
                                                                     GTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACC                          >  SRR4302540.422597/1‑94 (MQ=60)
                                                                     GTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACC                          <  SRR4302540.8810917/94‑1 (MQ=60)
                                                                     GTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACC                          <  SRR4302540.4869046/94‑1 (MQ=60)
                                                                     GTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACC                          >  SRR4302540.5471677/1‑94 (MQ=60)
                                                                     GTATCGTTTTCGCCGGATAAGCTCAATCAGGCCCTTGCCAGCCTGCTTGCGCAGCCGATGGTGCAGCACGGCCTGGTGCTCTCGACGTGCACCC                          >  SRR4302540.7256395/1‑94 (MQ=60)
                                                                       ATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCCACCGC                        >  SRR4302540.5339808/1‑94 (MQ=60)
                                                                       ATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGC                        >  SRR4302540.1064589/1‑94 (MQ=60)
                                                                         CGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCAC                      >  SRR4302540.3716388/1‑94 (MQ=60)
                                                                          GTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACG                     >  SRR4302540.1964488/1‑94 (MQ=60)
                                                                          GTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACG                     >  SRR4302540.6951299/1‑94 (MQ=60)
                                                                          GTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACG                     <  SRR4302540.7963387/94‑1 (MQ=60)
                                                                          GTTTTCGCCGGATAAGCTCAATCAGGCGCGTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACG                     >  SRR4302540.5351508/1‑94 (MQ=60)
                                                                            TTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGA                   >  SRR4302540.4840131/1‑94 (MQ=60)
                                                                              TCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAAC                 >  SRR4302540.687491/1‑94 (MQ=60)
                                                                               CGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACT                >  SRR4302540.3666861/1‑94 (MQ=60)
                                                                                GCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTAGACGTGCAACCGCACGGACCTT               >  SRR4302540.6406314/1‑94 (MQ=60)
                                                                                GCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTAGACGTGCAACCGCACGGAACTT               >  SRR4302540.6406312/1‑94 (MQ=60)
                                                                                 CCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCTTGGTGCTGTCGACGTGCACCCGCACGGAACTTT              >  SRR4302540.3563481/1‑94 (MQ=60)
                                                                                 CCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGGGCTTT              >  SRR4302540.2684900/1‑94 (MQ=60)
                                                                                 CCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTT              >  SRR4302540.2684899/1‑94 (MQ=60)
                                                                                 CCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTT              <  SRR4302540.7777504/94‑1 (MQ=60)
                                                                                  CGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTA             >  SRR4302540.9713760/1‑94 (MQ=60)
                                                                                  CGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTA             >  SRR4302540.775939/1‑94 (MQ=60)
                                                                                  CGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTA             <  SRR4302540.7644923/94‑1 (MQ=60)
                                                                                  CGGATAAGCTCAACCAGGCCCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTA             <  SRR4302540.7644924/94‑1 (MQ=60)
                                                                                    GATAAGCTCAAGCAGGCGCTTGACACCCTTCTTGCCCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATC           <  SRR4302540.9676615/94‑1 (MQ=60)
                                                                                      TAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTT         >  SRR4302540.7675578/1‑94 (MQ=60)
                                                                                      TAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTT         <  SRR4302540.1255282/94‑1 (MQ=60)
                                                                                      TAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTT         >  SRR4302540.4369926/1‑94 (MQ=60)
                                                                                       AAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTA        >  SRR4302540.5340255/1‑94 (MQ=60)
                                                                                        AGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAG       <  SRR4302540.8656607/94‑1 (MQ=60)
                                                                                         GCTCAATCAGGCGCTTGACAGGCTGCTTGCGCAGTCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC      <  SRR4302540.6962248/94‑1 (MQ=60)
                                                                                         GCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC      <  SRR4302540.2095176/94‑1 (MQ=60)
                                                                                         GCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC      >  SRR4302540.164254/1‑94 (MQ=60)
                                                                                         GCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC      >  SRR4302540.8772220/1‑94 (MQ=60)
                                                                                           TCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGT    >  SRR4302540.4342795/1‑94 (MQ=60)
                                                                                           TCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGT    >  SRR4302540.7078454/1‑94 (MQ=60)
                                                                                           TCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGT    >  SRR4302540.1573073/1‑94 (MQ=60)
                                                                                           TCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGT    >  SRR4302540.3000030/1‑94 (MQ=60)
                                                                                           TCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGT    >  SRR4302540.3000031/1‑94 (MQ=60)
                                                                                            CAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTT   <  SRR4302540.1833591/94‑1 (MQ=60)
                                                                                            CAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTT   <  SRR4302540.2909626/94‑1 (MQ=60)
                                                                                             AATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTG  >  SRR4302540.4168669/1‑94 (MQ=60)
                                                                                             AATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTG  <  SRR4302540.7955720/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTG  >  NC_000913/1262928‑1263114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑