Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,263,021	G→A	D29N (GAT→AAT)	hemA →	HemA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,263,021	0	G	A	100.0%	832.8 / NA	223	D29N (GAT→AAT)	hemA	HemA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (124/99); total (124/99)

Only 100 of 233 total aligned reads displayed.

GCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC  >  NC_000913/1262931‑1263110

gCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAAt                                                                                         >  2:3535365/1‑93 (MQ=255)

 cAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATc                                                                                        <  2:895791/93‑1 (MQ=255)

   ggcATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAg                                                                                      >  1:4068277/3‑93 (MQ=255)

   ggcATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAg                                                                                      <  1:1479967/91‑1 (MQ=255)

   ggcATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAg                                                                                      >  1:2409306/3‑93 (MQ=255)

     cATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGgc                                                                                    >  1:6254383/1‑93 (MQ=255)

     cATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGgc                                                                                    <  2:1894713/93‑1 (MQ=255)

       tGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGgcgc                                                                                  >  1:4854317/1‑93 (MQ=255)

       tGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGgcgc                                                                                  >  1:3788171/1‑93 (MQ=255)

        gACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCt                                                                                 >  2:5757913/1‑93 (MQ=255)

        gACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCt                                                                                 >  2:1639986/1‑93 (MQ=255)

         aaccTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATATGCTCACTCAGGCGCtt                                                                                >  2:3110011/3‑93 (MQ=255)

           ccTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGa                                                                              >  2:3166211/1‑93 (MQ=255)

           ccTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGa                                                                              >  2:1524217/1‑93 (MQ=255)

            cTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGAc                                                                             <  2:4039454/93‑1 (MQ=255)

            cTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGAc                                                                             >  1:5116755/1‑93 (MQ=255)

            cTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGAc                                                                             <  2:6205951/93‑1 (MQ=255)

              tttAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAg                                                                           <  1:5541959/93‑1 (MQ=255)

               ttAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGc                                                                          >  1:2114684/1‑93 (MQ=255)

                tAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGcc                                                                         >  2:1819301/1‑93 (MQ=255)

                tAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGcc                                                                         <  1:839826/93‑1 (MQ=255)

                 aGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCt                                                                        <  2:531274/93‑1 (MQ=255)

                   cACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGc                                                                      <  2:5334291/93‑1 (MQ=255)

                    aCTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCt                                                                     <  2:6234778/93‑1 (MQ=255)

                    aCTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCt                                                                     >  2:2759509/1‑93 (MQ=255)

                     cTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCtt                                                                    <  1:137935/93‑1 (MQ=255)

                     cTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGATCAATCAGGCGCTTGACAGCCTGCtt                                                                    >  2:2920069/1‑93 (MQ=255)

                      tCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTg                                                                   <  1:4875588/93‑1 (MQ=255)

                       cGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTgc                                                                  <  2:1323643/93‑1 (MQ=255)

                         gTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTgcgc                                                                >  2:4111461/1‑93 (MQ=255)

                         gTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTgcgc                                                                <  1:6000602/93‑1 (MQ=255)

                         gTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTgcgc                                                                >  2:5693707/1‑93 (MQ=255)

                          tATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCa                                                               <  2:336542/93‑1 (MQ=255)

                            tCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGc                                                             <  1:3098724/93‑1 (MQ=255)

                            tCAACCATAAAACGGCACATGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGc                                                             <  2:2418794/93‑1 (MQ=255)

                               aCCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGa                                                          >  2:4179802/1‑93 (MQ=255)

                               aCCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGa                                                          <  2:5726820/93‑1 (MQ=255)

                                ccATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGAt                                                         <  1:3829357/93‑1 (MQ=255)

                                 cATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATg                                                        <  2:377998/93‑1 (MQ=255)

                                 cATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATg                                                        <  1:2848869/93‑1 (MQ=255)

                                     aaaCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGc                                                    <  2:166653/93‑1 (MQ=255)

                                     aaaCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGc                                                    >  1:3690970/1‑93 (MQ=255)

                                      aaCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCa                                                   >  1:1319647/1‑93 (MQ=255)

                                      aaCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCa                                                   >  2:2964178/1‑93 (MQ=255)

                                       aCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAg                                                  <  2:4862078/93‑1 (MQ=255)

                                       aCGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAg                                                  <  1:5255811/93‑1 (MQ=255)

                                        cGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAgg                                                 <  1:5507308/93‑1 (MQ=255)

                                         ggCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAggg                                                <  2:5125173/93‑1 (MQ=255)

                                            aCCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACACCCTGCTTGCGCAGCCGATGGTGCAGggcgg                                             <  1:164107/93‑1 (MQ=255)

                                             ccTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGggcggc                                            >  2:4564527/1‑93 (MQ=255)

                                               tGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCgt                                          >  1:1816051/1‑93 (MQ=255)

                                               tGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCgt                                          >  1:2962467/1‑93 (MQ=255)

                                                 tATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCgtgg                                        >  1:1952638/1‑93 (MQ=255)

                                                  aTCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCgtggt                                       <  2:4742632/93‑1 (MQ=255)

                                                  aTCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCgtggg                                       >  1:1171732/1‑92 (MQ=255)

                                                       tGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATAAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTgg                                  >  1:2827164/1‑92 (MQ=255)

                                                           agaACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGAc                              >  2:146614/1‑93 (MQ=255)

                                                            gaACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACg                             >  1:4131291/1‑93 (MQ=255)

                                                            gaACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACg                             >  2:4695717/1‑93 (MQ=255)

                                                            gaACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACg                             >  2:1109893/1‑93 (MQ=255)

                                                               cGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGc                          <  1:3824066/93‑1 (MQ=255)

                                                                gtgtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCa                         >  2:3254071/1‑93 (MQ=255)

                                                                gtgtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCa                         >  2:2520802/1‑93 (MQ=255)

                                                                 tgtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCaa                        <  1:1331246/93‑1 (MQ=255)

                                                                  gtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAAc                       <  2:3153812/93‑1 (MQ=255)

                                                                  gtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAAc                       >  2:6075841/1‑93 (MQ=255)

                                                                  gtATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAAc                       >  2:6265436/1‑93 (MQ=255)

                                                                   tATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAAcc                      >  1:3457139/1‑93 (MQ=255)

                                                                   tATCGTGTTCGCCGGATAAGCTCAATCAGTCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAAcc                      <  1:3207556/93‑1 (MQ=255)

                                                                     tCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGc                    <  1:5777788/93‑1 (MQ=255)

                                                                     tCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGc                    >  2:826760/1‑93 (MQ=255)

                                                                       gTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCAc                  >  2:1678860/1‑93 (MQ=255)

                                                                       gTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCAc                  >  1:4636495/1‑93 (MQ=255)

                                                                        ttttCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCATGGCGGCGTGGTGCTGTCGACGTGCAACCGCACg                 >  2:4527777/1‑93 (MQ=255)

                                                                        ttttCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACg                 <  2:5980400/93‑1 (MQ=255)

                                                                        ttttCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACg                 >  2:4145082/1‑93 (MQ=255)

                                                                         tttCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACgg                >  1:732684/1‑93 (MQ=255)

                                                                           tCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGaa              <  2:3903414/93‑1 (MQ=255)

                                                                            cGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAAc             <  1:780532/93‑1 (MQ=255)

                                                                               cGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACttt          >  1:4527069/1‑93 (MQ=255)

                                                                               cGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACttt          >  2:1913367/1‑93 (MQ=255)

                                                                                ggATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTa         >  1:5201348/1‑93 (MQ=255)

                                                                                 gATAAGCTCAATCAGGCGCTTGACAGCATGCGTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTAt        <  1:3068737/93‑1 (MQ=255)

                                                                                  aTAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATc       <  2:6100879/93‑1 (MQ=255)

                                                                                  aTAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATc       <  1:2243668/93‑1 (MQ=255)

                                                                                   tAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCt      >  1:945656/1‑93 (MQ=255)

                                                                                   tAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCt      >  1:5650767/1‑93 (MQ=255)

                                                                                   tAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCt      <  1:3512217/93‑1 (MQ=255)

                                                                                   aaaGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCt      <  2:4415392/92‑1 (MQ=255)

                                                                                   aaaGCTCAATCAGGCGCTTGACAGCCGGCTTTCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCt      <  2:755942/92‑1 (MQ=255)

                                                                                    aaGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCtt     <  1:4743617/93‑1 (MQ=255)

                                                                                    aaGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCtt     >  1:1514441/1‑93 (MQ=255)

                                                                                     aGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTa    >  1:3280318/1‑93 (MQ=255)

                                                                                     aGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTa    >  1:1298477/1‑93 (MQ=255)

                                                                                      gCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAg   >  1:532570/1‑93 (MQ=255)

                                                                                      gCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAg   <  1:3956116/93‑1 (MQ=255)

                                                                                      gCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAg   >  1:261430/1‑93 (MQ=255)

                                                                                       cTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGc  <  2:2612455/93‑1 (MQ=255)

                                                                                       cTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGc  <  1:4945561/93‑1 (MQ=255)

                                                                                       cTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGc  <  1:4708152/93‑1 (MQ=255)

GCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC  >  NC_000913/1262931‑1263110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTT  >  NC_000913/1262928‑1263113

CCCGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCA                                                                                              >  SRR4302533.4973535/1‑94 (MQ=60)

CCCGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCA                                                                                              >  SRR4302533.1397849/1‑94 (MQ=60)

CCCGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCA                                                                                              <  SRR4302533.6298326/94‑1 (MQ=60)

 CCGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAA                                                                                             <  SRR4302533.3315824/94‑1 (MQ=60)

 CCGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAA                                                                                             >  SRR4302533.1893358/1‑94 (MQ=60)

  CGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAAT                                                                                            <  SRR4302533.2409191/94‑1 (MQ=60)

  CGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAAT                                                                                            <  SRR4302533.463313/94‑1 (MQ=60)

  CGCAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAAT                                                                                            <  SRR4302533.3874848/94‑1 (MQ=60)

    CAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCA                                                                                          >  SRR4302533.132962/1‑94 (MQ=60)

    CAGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCA                                                                                          >  SRR4302533.5061594/1‑94 (MQ=60)

     AGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAG                                                                                         <  SRR4302533.770401/94‑1 (MQ=60)

     AGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAG                                                                                         >  SRR4302533.2424188/1‑94 (MQ=60)

     AGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAG                                                                                         <  SRR4302533.272045/94‑1 (MQ=60)

     AGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAG                                                                                         >  SRR4302533.4093042/1‑94 (MQ=60)

     AGGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAG                                                                                         >  SRR4302533.4681916/1‑94 (MQ=60)

      GGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGG                                                                                        <  SRR4302533.4002545/94‑1 (MQ=60)

      GGCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGG                                                                                        <  SRR4302533.1488905/94‑1 (MQ=60)

       GCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGC                                                                                       >  SRR4302533.6291789/1‑94 (MQ=60)

         ATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGC                                                                                     >  SRR4302533.4895852/1‑94 (MQ=60)

         ATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGC                                                                                     >  SRR4302533.837924/1‑94 (MQ=60)

         ATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGC                                                                                     >  SRR4302533.3811370/1‑94 (MQ=60)

         ATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGC                                                                                     >  SRR4302533.4883736/1‑94 (MQ=60)

          TGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCT                                                                                    >  SRR4302533.3488852/1‑94 (MQ=60)

           GACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTT                                                                                   <  SRR4302533.3652134/94‑1 (MQ=60)

           GACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTT                                                                                   >  SRR4302533.4776483/1‑94 (MQ=60)

            ACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTG                                                                                  >  SRR4302533.4567650/1‑94 (MQ=60)

              CCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGAC                                                                                >  SRR4302533.1469701/1‑94 (MQ=60)

              CCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGAC                                                                                >  SRR4302533.5147678/1‑94 (MQ=60)

               CTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACA                                                                               <  SRR4302533.5354629/94‑1 (MQ=60)

               CTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACA                                                                               >  SRR4302533.3368942/1‑94 (MQ=60)

               CTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACA                                                                               <  SRR4302533.805303/94‑1 (MQ=60)

                 TTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGC                                                                             >  SRR4302533.2127726/1‑94 (MQ=60)

                 TTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGC                                                                             <  SRR4302533.5575430/94‑1 (MQ=60)

                 TTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGC                                                                             >  SRR4302533.137081/1‑94 (MQ=60)

                   TAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCT                                                                           <  SRR4302533.844739/94‑1 (MQ=60)

                        CTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTG                                                                      <  SRR4302533.138810/94‑1 (MQ=60)

                         TCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGC                                                                     <  SRR4302533.4905127/94‑1 (MQ=60)

                         TCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGC                                                                     <  SRR4302533.4905126/94‑1 (MQ=60)

                           GGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGC                                                                   >  SRR4302533.1004938/1‑94 (MQ=60)

                            GTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCA                                                                  <  SRR4302533.6036951/94‑1 (MQ=60)

                             TATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAG                                                                 <  SRR4302533.3482440/94‑1 (MQ=60)

                              ATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGC                                                                >  SRR4302533.4335579/1‑94 (MQ=60)

                               TCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCC                                                               <  SRR4302533.3117774/94‑1 (MQ=60)

                                 AACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGA                                                             >  SRR4302533.2003176/1‑94 (MQ=60)

                                 AACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGA                                                             >  SRR4302533.1671214/1‑94 (MQ=60)

                                   CCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATG                                                           <  SRR4302533.3852769/94‑1 (MQ=60)

                                   CCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATG                                                           >  SRR4302533.5997799/1‑94 (MQ=60)

                                    CATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGG                                                          <  SRR4302533.2866396/94‑1 (MQ=60)

                                    CATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGG                                                          <  SRR4302533.5621303/94‑1 (MQ=60)

                                     ATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGT                                                         <  SRR4302533.5752326/94‑1 (MQ=60)

                                      TAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTG                                                        >  SRR4302533.943133/1‑94 (MQ=60)

                                       AAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGC                                                       <  SRR4302533.320343/94‑1 (MQ=60)

                                       AAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGC                                                       >  SRR4302533.3713648/1‑94 (MQ=60)

                                        AAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCA                                                      >  SRR4302533.1327566/1‑94 (MQ=60)

                                          ACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGG                                                    <  SRR4302533.5287380/94‑1 (MQ=60)

                                           CGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGG                                                   <  SRR4302533.5540541/94‑1 (MQ=60)

                                            GGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGC                                                  >  SRR4302533.4762091/1‑94 (MQ=60)

                                              CACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGG                                                >  SRR4302533.1625365/1‑94 (MQ=60)

                                              CACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGG                                                >  SRR4302533.3767241/1‑94 (MQ=60)

                                               ACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGC                                               >  SRR4302533.5505471/1‑94 (MQ=60)

                                               ACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACACCCTGCTTGCGCAGCCGATGGTGCAGGGCGGC                                               <  SRR4302533.165151/94‑1 (MQ=60)

                                                 CTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGT                                             >  SRR4302533.5323207/1‑94 (MQ=60)

                                                 CTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGT                                             >  SRR4302533.2980577/1‑94 (MQ=60)

                                                 CTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGT                                             >  SRR4302533.1827137/1‑94 (MQ=60)

                                                 CTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGT                                             <  SRR4302533.2865423/94‑1 (MQ=60)

                                                   GTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGG                                           >  SRR4302533.1964539/1‑94 (MQ=60)

                                                    TATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGG                                          >  SRR4302533.1178765/1‑94 (MQ=60)

                                                         CTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATAAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGG                                     >  SRR4302533.2844559/1‑94 (MQ=60)

                                                          TGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTC                                    <  SRR4302533.231322/94‑1 (MQ=60)

                                                              AGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACG                                <  SRR4302533.3848174/94‑1 (MQ=60)

                                                              AGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACG                                >  SRR4302533.4156367/1‑94 (MQ=60)

                                                              AGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACG                                >  SRR4302533.1384740/1‑94 (MQ=60)

                                                              AGAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACG                                >  SRR4302533.1186493/1‑94 (MQ=60)

                                                               GAACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGT                               >  SRR4302533.2228916/1‑94 (MQ=60)

                                                                AACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTG                              >  SRR4302533.5571814/1‑94 (MQ=60)

                                                                AACGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTG                              <  SRR4302533.3225044/94‑1 (MQ=60)

                                                                AACGGGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTG                              <  SRR4302533.2983838/94‑1 (MQ=60)

                                                                  CGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCA                            <  SRR4302533.4026220/94‑1 (MQ=60)

                                                                  CGTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCA                            <  SRR4302533.3847445/94‑1 (MQ=60)

                                                                   GTGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAA                           >  SRR4302533.6139744/1‑94 (MQ=60)

                                                                    TGTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAAC                          <  SRR4302533.1339232/94‑1 (MQ=60)

                                                                     GTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACC                         >  SRR4302533.3815641/1‑94 (MQ=60)

                                                                     GTATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACC                         >  SRR4302533.3478483/1‑94 (MQ=60)

                                                                      TATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCG                        >  SRR4302533.3815516/1‑94 (MQ=60)

                                                                      TATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCG                        <  SRR4302533.5270398/94‑1 (MQ=60)

                                                                      TATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCG                        >  SRR4302533.3233516/1‑94 (MQ=60)

                                                                      TATCGTGTTCGCCGGATAAGCTCAATCAGTCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCG                        <  SRR4302533.3227308/94‑1 (MQ=60)

                                                                       ATCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGC                       >  SRR4302533.2879927/1‑94 (MQ=60)

                                                                        TCGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCA                      <  SRR4302533.5812687/94‑1 (MQ=60)

                                                                         CGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCAC                     <  SRR4302533.4812762/94‑1 (MQ=60)

                                                                         CGTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCAC                     >  SRR4302533.4664590/1‑94 (MQ=60)

                                                                         CGTTTTCGCCGGATAAGCTCAACCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCAC                     <  SRR4302533.1523739/94‑1 (MQ=60)

                                                                          GTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACG                    >  SRR4302533.6342663/1‑94 (MQ=60)

                                                                          GTTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACG                    <  SRR4302533.3094090/94‑1 (MQ=60)

                                                                           TTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGG                   >  SRR4302533.736994/1‑94 (MQ=60)

                                                                           TTTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGG                   <  SRR4302533.5214967/94‑1 (MQ=60)

                                                                            TTTCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGA                  >  SRR4302533.2958580/1‑94 (MQ=60)

                                                                              TCGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAAC                >  SRR4302533.6226139/1‑94 (MQ=60)

                                                                               CGCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACT               <  SRR4302533.785130/94‑1 (MQ=60)

                                                                                GCCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTT              >  SRR4302533.2095830/1‑94 (MQ=60)

                                                                                 CCGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTT             >  SRR4302533.4554481/1‑94 (MQ=60)

                                                                                  CGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTA            <  SRR4302533.5664565/94‑1 (MQ=60)

                                                                                  CGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTA            >  SRR4302533.5232699/1‑94 (MQ=60)

                                                                                  CGGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTA            >  SRR4302533.1306693/1‑94 (MQ=60)

                                                                                   GGATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTAT           >  SRR4302533.4607556/1‑94 (MQ=60)

                                                                                    GATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATC          >  SRR4302533.1901293/1‑94 (MQ=60)

                                                                                    GATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATC          >  SRR4302533.2555817/1‑94 (MQ=60)

                                                                                    GATAAGCTCAATCAGGCGCTTGACAGCATGCGTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATC          <  SRR4302533.3087580/94‑1 (MQ=60)

                                                                                     ATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCT         <  SRR4302533.2257535/94‑1 (MQ=60)

                                                                                     ATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCT         >  SRR4302533.5684925/1‑94 (MQ=60)

                                                                                     ATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCT         >  SRR4302533.951217/1‑94 (MQ=60)

                                                                                     ATAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCAGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCT         <  SRR4302533.3944228/94‑1 (MQ=60)

                                                                                      TAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTT        >  SRR4302533.4139436/1‑94 (MQ=60)

                                                                                      TAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTT        >  SRR4302533.1523564/1‑94 (MQ=60)

                                                                                      TAAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTT        <  SRR4302533.3533868/94‑1 (MQ=60)

                                                                                       AAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTA       >  SRR4302533.1306291/1‑94 (MQ=60)

                                                                                       AAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTA       <  SRR4302533.4772373/94‑1 (MQ=60)

                                                                                       AAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTA       <  SRR4302533.1522236/94‑1 (MQ=60)

                                                                                       AAGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTA       >  SRR4302533.3300569/1‑94 (MQ=60)

                                                                                        AGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAG      >  SRR4302533.263016/1‑94 (MQ=60)

                                                                                        AGCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAG      >  SRR4302533.535738/1‑94 (MQ=60)

                                                                                         GCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC     <  SRR4302533.3980232/94‑1 (MQ=60)

                                                                                         GCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC     <  SRR4302533.5087904/94‑1 (MQ=60)

                                                                                         GCTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGC     <  SRR4302533.260952/94‑1 (MQ=60)

                                                                                          CTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCG    <  SRR4302533.4975494/94‑1 (MQ=60)

                                                                                          CTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCG    >  SRR4302533.2079324/1‑94 (MQ=60)

                                                                                          CTCAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCG    <  SRR4302533.4736688/94‑1 (MQ=60)

                                                                                            CAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTT  >  SRR4302533.1505661/1‑94 (MQ=60)

                                                                                            CAATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTT  >  SRR4302533.2992559/1‑94 (MQ=60)

CCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTT  >  NC_000913/1262928‑1263113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`