Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,279,780	T→C	W232R (TGG→CGG)	narG →	nitrate reductase 1 alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,279,780	0	T	C	99.5%	655.9 / ‑5.1	208	W232R (TGG→CGG)	narG	nitrate reductase 1 alpha subunit
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (96/109); total (96/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

Only 100 of 215 total aligned reads displayed.

TCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCG  >  NC_000913/1279690‑1279868

tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCgg                                                                                        >  1:9465415/1‑93 (MQ=255)

 cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCggg                                                                                       >  1:5334008/1‑93 (MQ=255)

 cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCggg                                                                                       <  2:5504359/93‑1 (MQ=255)

  gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCgggg                                                                                      >  2:1143772/1‑93 (MQ=255)

     tGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGa                                                                                   >  2:10526/1‑93 (MQ=255)

         cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCCAACCCGGGGCGAGCaa                                                                               >  2:6649963/1‑93 (MQ=255)

          gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCaaa                                                                              >  1:1146056/1‑93 (MQ=255)

            tATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACt                                                                            <  2:7476593/93‑1 (MQ=255)

            tATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACt                                                                            >  2:9020561/1‑93 (MQ=255)

              tctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGa                                                                          <  1:4289669/93‑1 (MQ=255)

              tctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGa                                                                          <  1:5394759/93‑1 (MQ=255)

                tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACg                                                                        >  1:2944847/1‑93 (MQ=255)

                  tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTa                                                                      <  1:7149290/93‑1 (MQ=255)

                   cGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTAc                                                                     >  1:6068563/1‑93 (MQ=255)

                   cGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTAc                                                                     <  1:9677506/93‑1 (MQ=255)

                    gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTAcc                                                                    >  2:8168638/1‑93 (MQ=255)

                     cTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCg                                                                   >  1:7342332/1‑93 (MQ=255)

                      tGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCgg                                                                  >  2:2238211/1‑93 (MQ=255)

                      tGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCgg                                                                  >  2:6878635/1‑93 (MQ=255)

                      tGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCgg                                                                  >  2:7981084/1‑93 (MQ=255)

                       gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGa                                                                 >  1:2685741/1‑93 (MQ=255)

                        aTTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGaa                                                                <  1:9276378/93‑1 (MQ=255)

                         ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAAt                                                               >  2:9336383/1‑93 (MQ=255)

                          tGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATc                                                              <  1:6812578/93‑1 (MQ=255)

                          tGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATc                                                              <  2:46093/93‑1 (MQ=255)

                          tGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATc                                                              <  2:7805783/93‑1 (MQ=255)

                           ggCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATct                                                             >  2:2719802/1‑93 (MQ=255)

                           ggCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATct                                                             <  1:122920/93‑1 (MQ=255)

                            gCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATctg                                                            >  1:2066100/1‑93 (MQ=255)

                              ggTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATctgct                                                          <  1:4880864/93‑1 (MQ=255)

                               gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATctgctg                                                         >  1:9311287/1‑93 (MQ=255)

                               gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATctgctg                                                         >  2:3965324/1‑93 (MQ=255)

                               gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATctgctg                                                         >  2:9588105/1‑93 (MQ=255)

                                tACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGa                                                        >  1:4098348/1‑93 (MQ=255)

                                tACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGa                                                        <  1:3531689/93‑1 (MQ=255)

                                 tcTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGAc                                                       <  2:6841647/92‑1 (MQ=255)

                                 aCTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGAc                                                       <  1:4675738/93‑1 (MQ=255)

                                   ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTg                                                     <  2:1399275/93‑1 (MQ=255)

                                   ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTg                                                     >  2:5017634/1‑93 (MQ=255)

                                   ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTg                                                     <  1:4394152/93‑1 (MQ=255)

                                    tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTTACGTACCGGAATCTGCTGACTgg                                                    >  2:5609216/1‑93 (MQ=255)

                                    tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg                                                    >  1:7785135/1‑93 (MQ=255)

                                    tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg                                                    >  2:3699864/1‑93 (MQ=255)

                                     gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGt                                                   >  1:4164205/1‑93 (MQ=255)

                                      cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTa                                                  <  2:3166438/93‑1 (MQ=255)

                                        tAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACa                                                <  1:1399929/93‑1 (MQ=255)

                                        tAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACa                                                <  1:4004746/93‑1 (MQ=255)

                                         aaGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACaa                                               <  1:6633674/93‑1 (MQ=255)

                                          aGCTTCTACGACTGGTCCGGCGACTTTCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAAc                                              <  1:7532511/93‑1 (MQ=25)

                                          aGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAAc                                              >  2:8486949/1‑93 (MQ=255)

                                          aGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAAc                                              <  2:2798448/93‑1 (MQ=255)

                                           gCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACt                                             <  1:6324766/93‑1 (MQ=255)

                                            cTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc                                            >  2:2626065/1‑93 (MQ=255)

                                             ttCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTcc                                           >  2:2096455/1‑93 (MQ=255)

                                             ttCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTcc                                           >  1:8060243/1‑93 (MQ=255)

                                             ttCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTcc                                           <  2:6189070/93‑1 (MQ=255)

                                              tCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCa                                          >  1:2793791/1‑93 (MQ=255)

                                              tCTACGACTGGTACTGCGACTTGCCTCCTGCGTCGCCGCAAAACCGGGGCGAGCCAACTGACGTACCGGAATCTGCTGAATGGTACAACTCCa                                          >  2:6377053/1‑93 (MQ=21)

                                                  cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTa                                      >  1:5626275/1‑93 (MQ=255)

                                                  cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTa                                      >  2:7417413/1‑93 (MQ=255)

                                                   gACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACCCCAGCTAc                                     >  2:3392705/1‑93 (MQ=255)

                                                    aCTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAca                                    >  2:8367451/1‑93 (MQ=255)

                                                    aCTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAca                                    <  1:1741234/93‑1 (MQ=255)

                                                     cTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcat                                   >  1:8913462/1‑93 (MQ=255)

                                                       ggTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatca                                 <  2:8163434/93‑1 (MQ=255)

                                                       ggTACTGCCACTTGCCTCCTGCGTCTCCGCAAGCCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatca                                 <  1:2768993/93‑1 (MQ=255)

                                                           cTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc                             <  2:1756378/93‑1 (MQ=255)

                                                           cTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc                             >  1:7953734/1‑93 (MQ=255)

                                                            tGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGcc                            <  2:2556133/93‑1 (MQ=255)

                                                            tGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGcc                            >  1:7504729/1‑93 (MQ=255)

                                                              cGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg                          >  1:9173201/1‑93 (MQ=255)

                                                              cGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg                          <  1:6585579/93‑1 (MQ=255)

                                                              cGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg                          <  1:8494461/93‑1 (MQ=255)

                                                              cGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg                          <  1:3541747/93‑1 (MQ=255)

                                                               gACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgg                         >  1:1221193/1‑93 (MQ=255)

                                                                aCTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTggg                        <  2:5208750/93‑1 (MQ=255)

                                                                aCTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTggg                        >  1:8031105/1‑93 (MQ=255)

                                                                 cTTGCCTCCTGCGTCTCCGCAAGCCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCGgggg                       <  2:645421/93‑1 (MQ=255)

                                                                   tGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGt                     <  1:1864489/93‑1 (MQ=255)

                                                                   tGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGt                     <  1:7075786/93‑1 (MQ=255)

                                                                   tGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGt                     >  2:6034057/1‑93 (MQ=255)

                                                                     cctcctGCGTCTCCGCAAACCCGGGGCGGGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa                   >  1:8752836/1‑93 (MQ=255)

                                                                      ctcctGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaa                  <  1:766046/93‑1 (MQ=255)

                                                                        cctGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAAc                <  1:9033020/93‑1 (MQ=255)

                                                                         ctGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACg               >  1:9855628/1‑93 (MQ=255)

                                                                         ctGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACg               <  1:121495/93‑1 (MQ=255)

                                                                          tGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGt              <  2:10007660/93‑1 (MQ=255)

                                                                          tGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGt              <  1:941229/93‑1 (MQ=255)

                                                                           gCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTg             <  1:3332806/93‑1 (MQ=255)

                                                                            cGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc            <  1:3189372/93‑1 (MQ=255)

                                                                              tctcCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGccc          >  2:8656333/1‑92 (MQ=255)

                                                                               ctcCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGc         <  2:3315076/93‑1 (MQ=255)

                                                                                tcCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa        >  1:1772168/1‑93 (MQ=255)

                                                                                tcCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa        <  2:758812/93‑1 (MQ=255)

                                                                                tcCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa        <  2:2440764/93‑1 (MQ=255)

                                                                                tcCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa        >  1:9008069/1‑93 (MQ=255)

                                                                                 ccGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAg       <  1:6600229/93‑1 (MQ=255)

                                                                                 ccGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAg       >  1:4727544/1‑93 (MQ=255)

                                                                                  cGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGa      <  1:7468055/93‑1 (MQ=255)

                                                                                  cGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGa      <  1:1038369/93‑1 (MQ=255)

TCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCG  >  NC_000913/1279690‑1279868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCC  >  NC_000913/1279688‑1279873

CATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCG                                                                                              <  SRR4302540.1044476/94‑1 (MQ=60)

CATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCG                                                                                              >  SRR4302540.10022469/1‑94 (MQ=60)

 ATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGG                                                                                             >  SRR4302540.9577709/1‑94 (MQ=60)

 ATCGGGTGCACGCTATCTCTCGCTGATTGGCGGCACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGG                                                                                             >  SRR4302540.9577708/1‑94 (MQ=60)

  TCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGG                                                                                            >  SRR4302540.5392467/1‑94 (MQ=60)

    GGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGC                                                                                          >  SRR4302540.8728478/1‑94 (MQ=60)

       TGCACGCTCTGTCTCGCGGATTTGCGGTACTGGCTTAAGCTTCTACGACTGGTACTGGGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAG                                                                                       <  SRR4302540.8232178/94‑1 (MQ=60)

         CACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCA                                                                                     <  SRR4302540.3024387/94‑1 (MQ=60)

          ACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAA                                                                                    >  SRR4302540.7755188/1‑94 (MQ=60)

           CGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAA                                                                                   >  SRR4302540.1158019/1‑94 (MQ=60)

           CGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAA                                                                                   >  SRR4302540.6092284/1‑94 (MQ=60)

           CGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAA                                                                                   >  SRR4302540.7314834/1‑94 (MQ=60)

            GCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAAC                                                                                  >  SRR4302540.1152759/1‑94 (MQ=60)

            GCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAAA                                                                                  >  SRR4302540.7699552/1‑94 (MQ=60)

              TATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGTCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTG                                                                                >  SRR4302540.1712540/1‑94 (MQ=60)

              TATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTG                                                                                >  SRR4302540.1234598/1‑94 (MQ=60)

                TCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGAC                                                                              <  SRR4302540.4336337/94‑1 (MQ=60)

                TCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGAC                                                                              <  SRR4302540.4336338/94‑1 (MQ=60)

                TCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGAC                                                                              <  SRR4302540.5454076/94‑1 (MQ=60)

                 CTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACG                                                                             >  SRR4302540.2977230/1‑94 (MQ=60)

                    TCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTAC                                                                          >  SRR4302540.6136032/1‑94 (MQ=60)

                    TCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTAC                                                                          <  SRR4302540.7228961/94‑1 (MQ=60)

                    TCGCTGATTGGCGGTACTTGCTTAAGCTGCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTAC                                                                          <  SRR4302540.9178614/94‑1 (MQ=60)

                     CGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACC                                                                         <  SRR4302540.9791366/94‑1 (MQ=60)

                      GCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCG                                                                        <  SRR4302540.4048229/94‑1 (MQ=60)

                      GCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCG                                                                        >  SRR4302540.7424256/1‑94 (MQ=60)

                        TGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGA                                                                      >  SRR4302540.2714775/1‑94 (MQ=60)

                        TGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGA                                                                      >  SRR4302540.8035596/1‑94 (MQ=60)

                         GATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGAAAACTGACGTACCGGAA                                                                     >  SRR4302540.4542108/1‑94 (MQ=60)

                          ATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAAT                                                                    <  SRR4302540.9387280/94‑1 (MQ=60)

                           TTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATC                                                                   <  SRR4302540.8603142/94‑1 (MQ=60)

                            TGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCT                                                                  <  SRR4302540.6887963/94‑1 (MQ=60)

                             GGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTG                                                                 >  SRR4302540.2088329/1‑94 (MQ=60)

                             GGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTG                                                                 <  SRR4302540.5405513/94‑1 (MQ=60)

                             GGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTG                                                                 <  SRR4302540.124230/94‑1 (MQ=60)

                               CGGTACTTGCTTAATCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCT                                                               <  SRR4302540.309298/94‑1 (MQ=60)

                               CGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCT                                                               <  SRR4302540.8052479/94‑1 (MQ=60)

                               CGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCT                                                               <  SRR4302540.9100515/94‑1 (MQ=60)

                                GGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTG                                                              <  SRR4302540.9999871/94‑1 (MQ=60)

                                GGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTG                                                              >  SRR4302540.9422440/1‑94 (MQ=60)

                                GGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTG                                                              <  SRR4302540.4933272/94‑1 (MQ=60)

                                 GTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGA                                                             >  SRR4302540.4142554/1‑94 (MQ=60)

                                  TACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGAC                                                            <  SRR4302540.3570237/94‑1 (MQ=60)

                                  TACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGAC                                                            <  SRR4302540.3570238/94‑1 (MQ=60)

                                  GACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGAC                                                            <  SRR4302540.6399251/94‑1 (MQ=60)

                                   ACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACT                                                           <  SRR4302540.4725769/94‑1 (MQ=60)

                                    CTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTG                                                          >  SRR4302540.2306975/1‑94 (MQ=60)

                                     TTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGG                                                         >  SRR4302540.7876133/1‑94 (MQ=60)

                                     TTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGG                                                         <  SRR4302540.4441832/94‑1 (MQ=60)

                                      TGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGT                                                        >  SRR4302540.4209340/1‑94 (MQ=60)

                                       GCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTA                                                       <  SRR4302540.6813697/94‑1 (MQ=60)

                                        aatacgctacagcgactagtactgcgactagcataaaacgaCTCCGAAAACCCGGGGCGAGCCAACTGACGTACCGGAATCTGCTGACTaatac                                                      <  SRR4302540.9037825/53‑6 (MQ=60)

                                          TAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAA                                                    <  SRR4302540.4047860/94‑1 (MQ=60)

                                          TAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAA                                                    <  SRR4302540.1414977/94‑1 (MQ=60)

                                           AAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAAC                                                   <  SRR4302540.6707091/94‑1 (MQ=60)

                                            AGCTTCTACGACTGGTCCGGCGACTTTCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACT                                                  <  SRR4302540.7616855/94‑1 (MQ=60)

                                             GCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTC                                                 <  SRR4302540.6395201/94‑1 (MQ=60)

                                              CTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCC                                                >  SRR4302540.8153813/1‑94 (MQ=60)

                                              CTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCC                                                >  SRR4302540.5487847/1‑94 (MQ=60)

                                               TTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCA                                               >  SRR4302540.2823954/1‑94 (MQ=60)

                                               TTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCA                                               >  SRR4302540.2823955/1‑94 (MQ=60)

                                               TTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCA                                               >  SRR4302540.4402590/1‑94 (MQ=60)

                                                TCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGCCTGGTACAACTCCAG                                              >  SRR4302540.6742387/1‑94 (MQ=60)

                                                TCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAG                                              <  SRR4302540.3340597/94‑1 (MQ=60)

                                                 CTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGC                                             >  SRR4302540.414899/1‑94 (MQ=60)

                                                  TACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCT                                            <  SRR4302540.6203017/94‑1 (MQ=60)

                                                   ACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTA                                           >  SRR4302540.5688205/1‑94 (MQ=60)

                                                   ACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTA                                           <  SRR4302540.8144847/94‑1 (MQ=60)

                                                    CGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAC                                          >  SRR4302540.5434877/1‑94 (MQ=60)

                                                     GACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACA                                         >  SRR4302540.1199998/1‑94 (MQ=60)

                                                      ACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACAT                                        >  SRR4302540.9016683/1‑94 (MQ=60)

                                                      ACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACAT                                        <  SRR4302540.1760073/94‑1 (MQ=60)

                                                       CTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATC                                       <  SRR4302540.9583754/94‑1 (MQ=60)

                                                        TGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCAGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCA                                      <  SRR4302540.4730716/94‑1 (MQ=60)

                                                         GGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCAT                                     <  SRR4302540.7197881/94‑1 (MQ=60)

                                                         GGTACTGCCACTTGCCTCCTGCGTCTCCGCAAGCCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCAT                                     <  SRR4302540.2798917/94‑1 (MQ=60)

                                                            ACTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGC                                  >  SRR4302540.8046446/1‑94 (MQ=60)

                                                             CTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCC                                 >  SRR4302540.7588606/1‑94 (MQ=60)

                                                             CTGCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGCCCAACTCCAGCTACATCATCGCC                                 <  SRR4302540.1789070/94‑1 (MQ=60)

                                                               GCGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTG                               >  SRR4302540.9283235/1‑94 (MQ=60)

                                                                CGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGG                              <  SRR4302540.3580428/94‑1 (MQ=60)

                                                                CGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGG                              <  SRR4302540.6658524/94‑1 (MQ=60)

                                                                CGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGG                              <  SRR4302540.9392234/94‑1 (MQ=60)

                                                                CGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGG                              >  SRR4302540.1233692/1‑94 (MQ=60)

                                                                CGACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGG                              <  SRR4302540.8593165/94‑1 (MQ=60)

                                                                 GACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGG                             >  SRR4302540.8124475/1‑94 (MQ=60)

                                                                 GACTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGG                             >  SRR4302540.9965332/1‑94 (MQ=60)

                                                                   CTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGG                           <  SRR4302540.4187959/94‑1 (MQ=60)

                                                                   CTTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGG                           <  SRR4302540.949619/94‑1 (MQ=60)

                                                                    TTGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGT                          <  SRR4302540.2081791/94‑1 (MQ=60)

                                                                     TGCCTTCTGCGTCTCCGCAAGCGCGGGGGGAGCAAACGCACGTACCGGATTCGGCTGACTGGTACAACTCCAGCTACATCATCGCCGGGGGGTC                         <  SRR4302540.6668471/94‑1 (MQ=23)

                                                                     TGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTC                         <  SRR4302540.7154522/94‑1 (MQ=60)

                                                                     TGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTC                         <  SRR4302540.3895368/94‑1 (MQ=60)

                                                                     TGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTC                         <  SRR4302540.6668470/94‑1 (MQ=60)

                                                                     TGCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTC                         <  SRR4302540.1884927/94‑1 (MQ=60)

                                                                      TCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCA                        <  SRR4302540.337311/94‑1 (MQ=60)

                                                                      GCCTCCTGCGTCTCCGCAAACCCGGGGCGGGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCA                        >  SRR4302540.8854380/1‑94 (MQ=60)

                                                                      GCCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCA                        >  SRR4302540.8854381/1‑94 (MQ=60)

                                                                       CCTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAA                       <  SRR4302540.185306/94‑1 (MQ=60)

                                                                        CTCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAA                      <  SRR4302540.774205/94‑1 (MQ=60)

                                                                         TCCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAAC                     <  SRR4302540.9460120/94‑1 (MQ=60)

                                                                          CCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACG                    >  SRR4302540.9970809/1‑94 (MQ=60)

                                                                          CCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACG                    <  SRR4302540.9141422/94‑1 (MQ=60)

                                                                          CCTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACG                    <  SRR4302540.9141421/94‑1 (MQ=60)

                                                                           CTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGT                   <  SRR4302540.122796/94‑1 (MQ=60)

                                                                           CTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGT                   <  SRR4302540.122795/94‑1 (MQ=60)

                                                                           CTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGT                   <  SRR4302540.1292397/94‑1 (MQ=60)

                                                                           CTGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGT                   >  SRR4302540.5119067/1‑94 (MQ=60)

                                                                            TGCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTG                  <  SRR4302540.951063/94‑1 (MQ=60)

                                                                             GCGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGC                 <  SRR4302540.3369401/94‑1 (MQ=60)

                                                                              CGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCC                <  SRR4302540.2804369/94‑1 (MQ=60)

                                                                              CGTCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCC                <  SRR4302540.3224016/94‑1 (MQ=60)

                                                                              CGGCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCC                <  SRR4302540.2722426/94‑1 (MQ=60)

                                                                                TCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGC              >  SRR4302540.7598750/1‑94 (MQ=60)

                                                                                TCTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGC              >  SRR4302540.7598749/1‑94 (MQ=60)

                                                                                 CTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCA             >  SRR4302540.9116138/1‑94 (MQ=60)

                                                                                 CTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCA             >  SRR4302540.1784320/1‑94 (MQ=60)

                                                                                 CTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCA             >  SRR4302540.6968843/1‑94 (MQ=60)

                                                                                 CTCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCA             >  SRR4302540.1791401/1‑94 (MQ=60)

                                                                                  TCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAG            >  SRR4302540.4778038/1‑94 (MQ=60)

                                                                                  TCCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAG            >  SRR4302540.4778036/1‑94 (MQ=60)

                                                                                   CCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGA           <  SRR4302540.6673318/94‑1 (MQ=60)

                                                                                   CCGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGA           <  SRR4302540.6673319/94‑1 (MQ=60)

                                                                                    CGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGAC          <  SRR4302540.1048929/94‑1 (MQ=60)

                                                                                    CGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGAC          <  SRR4302540.7582063/94‑1 (MQ=60)

                                                                                    CGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGAC          <  SRR4302540.7551369/94‑1 (MQ=60)

                                                                                    CGCAAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGAC          <  SRR4302540.7551368/94‑1 (MQ=60)

                                                                                       AAACCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCG       >  SRR4302540.9357948/1‑94 (MQ=60)

                                                                                          CCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAC    <  SRR4302540.9431432/94‑1 (MQ=60)

                                                                                          CCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAC    <  SRR4302540.2253328/94‑1 (MQ=60)

                                                                                          CCCGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAC    >  SRR4302540.3384343/1‑94 (MQ=60)

                                                                                            CGGGGCGAGCAAACTGACGTAGCGGAATCTGCTGACTGGTACAACTCCACCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCC  <  SRR4302540.2223328/94‑1 (MQ=60)

                                                                                            CGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCC  <  SRR4302540.2223329/94‑1 (MQ=60)

                                                                                            CGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCC  >  SRR4302540.4491557/1‑94 (MQ=60)

CATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCC  >  NC_000913/1279688‑1279873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`