Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,318,373 A→G C346R (TGT→CGT)  trpD ← anthranilate synthase component II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,318,3730AG91.3% 578.7 / 35.4 218C346R (TGT→CGT) trpDanthranilate synthase component II
Reads supporting (aligned to +/- strand):  ref base A (8/11);  new base G (92/107);  total (100/118)
Fisher's exact test for biased strand distribution p-value = 8.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 225 total aligned reads displayed.

AGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAG  >  NC_000913/1318282‑1318462
                                                                                           |                                                                                         
 gCACATTGATCAGGTGGCGGGTGTTCAGTTGCTGGCGAACCGGCATCGCGTGGCTGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTa                                                                                         <  1:1805262/93‑1 (MQ=255)
 gCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTa                                                                                         >  1:1396698/1‑93 (MQ=255)
 gCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTa                                                                                         <  2:8854499/93‑1 (MQ=255)
  cacaTTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATac                                                                                        <  1:766224/93‑1 (MQ=255)
   acaTTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGGGTGATACTTCGGCGCAAAGAGGAAACGTaca                                                                                       >  1:2522492/1‑93 (MQ=255)
    caTTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTacac                                                                                      <  2:7689194/93‑1 (MQ=255)
    caTTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTacac                                                                                      <  1:3805936/93‑1 (MQ=255)
     aTTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACAcc                                                                                     <  2:4570260/93‑1 (MQ=255)
      ttGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCt                                                                                    >  2:2884559/1‑93 (MQ=255)
      ttGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTAAACCt                                                                                    >  1:3437623/1‑93 (MQ=255)
        gAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTaa                                                                                  <  1:275305/93‑1 (MQ=255)
         aaCAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACAGCTAAc                                                                                 >  2:3084534/1‑93 (MQ=255)
          aCAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACt                                                                                >  1:27314/1‑93 (MQ=255)
           cAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTc                                                                               <  2:5762513/93‑1 (MQ=255)
           cAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTc                                                                               <  1:9256885/93‑1 (MQ=255)
            aGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCa                                                                              <  2:3058982/93‑1 (MQ=255)
            aGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCa                                                                              >  1:9023601/1‑93 (MQ=255)
               gggCGGGTGTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATcc                                                                           <  1:9925722/91‑1 (MQ=255)
                tGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCa                                                                          >  2:7153218/1‑93 (MQ=255)
                ggctggTTTGCAGTTGCTGGCGAACCGGCATCGCGTGGCTGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCa                                                                          <  1:536823/89‑1 (MQ=255)
                 gCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAg                                                                         <  2:2027041/93‑1 (MQ=255)
                     gTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGcc                                                                     <  2:2756483/93‑1 (MQ=255)
                     gTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGcc                                                                     >  1:2307999/1‑93 (MQ=255)
                      ttttGAGTTGCTGGCGAGCCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCACAAAGAGGAAACGTACACCTAACGCATCCAGCGCCt                                                                    <  2:5626431/93‑1 (MQ=255)
                       tttCAGTTGCTGGCGAGCCGGCATCGAGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTg                                                                   <  2:8272076/93‑1 (MQ=255)
                       tttCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGCAACGTACACCTAACTCATCCAGCGCCTg                                                                   >  1:513768/1‑93 (MQ=255)
                       tttCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTg                                                                   <  1:4162888/93‑1 (MQ=255)
                       tttCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTg                                                                   <  2:4401498/93‑1 (MQ=255)
                       tttCAGTAGCTGGCTACCCGGCAACGCGTGGCAGAATCCGGTATGATGCTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTg                                                                   <  2:8566607/93‑1 (MQ=255)
                        ttCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTgg                                                                  <  2:8939144/93‑1 (MQ=255)
                        ttCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTgg                                                                  >  2:766645/1‑93 (MQ=255)
                           aGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGgcgc                                                               >  2:1430956/1‑93 (MQ=255)
                            gTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGcgcg                                                              >  2:4000360/1‑93 (MQ=255)
                             ttGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGa                                                             >  2:915173/1‑93 (MQ=255)
                              tGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGAt                                                            <  2:5067711/93‑1 (MQ=255)
                              tGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGAt                                                            <  2:5325381/93‑1 (MQ=255)
                                gtGGCGAACCGGCATCGCGTGGCGGAATCCGGTATGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGAttt                                                          <  1:4185059/92‑1 (MQ=255)
                                  ggCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCGTGGCGCGATTTAt                                                        <  1:6716130/93‑1 (MQ=255)
                                    cGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCg                                                      <  2:7923498/93‑1 (MQ=255)
                                     gAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCgg                                                     <  1:2271684/93‑1 (MQ=255)
                                      aaCCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGc                                                    >  1:6078892/1‑93 (MQ=255)
                                      aaCCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGc                                                    >  2:9891652/1‑93 (MQ=255)
                                      aaCCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGc                                                    <  1:2204098/93‑1 (MQ=255)
                                        ccGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGt                                                  >  1:2598774/1‑93 (MQ=255)
                                        ccGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGt                                                  >  1:1044054/1‑93 (MQ=255)
                                           gCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCa                                               >  1:3139323/1‑93 (MQ=255)
                                           gCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCa                                               >  2:5933463/1‑93 (MQ=255)
                                            cATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCat                                              <  1:9657775/93‑1 (MQ=255)
                                            cATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCat                                              <  2:4296823/93‑1 (MQ=255)
                                             aTCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCata                                             <  1:102120/93‑1 (MQ=255)
                                              tCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCatat                                            >  2:8768660/1‑93 (MQ=255)
                                              tCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCatat                                            >  2:5679962/1‑93 (MQ=255)
                                              tCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCatat                                            <  2:6987226/93‑1 (MQ=255)
                                               cgcgTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATc                                           <  2:7516746/93‑1 (MQ=255)
                                                 cgTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCaa                                         <  1:8874969/93‑1 (MQ=255)
                                                   tGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGa                                       <  2:9686910/93‑1 (MQ=255)
                                                   tGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGa                                       >  2:5904362/1‑93 (MQ=255)
                                                   tGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGa                                       <  1:8085062/93‑1 (MQ=255)
                                                    ggCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGAt                                      <  1:5809653/93‑1 (MQ=255)
                                                     gCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGAtt                                     >  2:3508160/1‑93 (MQ=255)
                                                     gCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGAtt                                     >  1:8209655/1‑93 (MQ=255)
                                                      cGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTa                                    >  1:342345/1‑93 (MQ=255)
                                                      cGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTa                                    <  1:6982030/93‑1 (MQ=255)
                                                         aaTCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATa                                 <  2:3377427/93‑1 (MQ=255)
                                                          aTCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATAc                                <  1:1311564/93‑1 (MQ=255)
                                                          aTCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATAc                                <  2:1702772/93‑1 (MQ=255)
                                                           tCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATAcc                               >  2:3887741/1‑93 (MQ=255)
                                                           tCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATAcc                               <  2:4833795/93‑1 (MQ=255)
                                                             cGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGa                             <  1:8324329/93‑1 (MQ=255)
                                                             cGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGa                             <  2:7048573/93‑1 (MQ=255)
                                                             cGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGa                             <  1:962187/93‑1 (MQ=255)
                                                             cGGTCTGATACTTCGGCGCAAAGAGGAACCGTACACCTAACTCATCCAGCGGCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGa                             <  1:440500/93‑1 (MQ=255)
                                                              ggTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGaa                            <  1:2071441/93‑1 (MQ=255)
                                                              ggTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGaa                            <  1:1888753/93‑1 (MQ=255)
                                                              ggTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGaa                            <  2:375235/93‑1 (MQ=255)
                                                              ggTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGaa                            <  1:6434292/93‑1 (MQ=255)
                                                               gtgtGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAc                           >  2:6251090/1‑93 (MQ=255)
                                                               gtgtGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAc                           <  1:8486813/93‑1 (MQ=255)
                                                                tgtgATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAcg                          >  1:5183659/1‑93 (MQ=255)
                                                                 gtgATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTTATACCGAAcgc                         >  1:3939281/1‑93 (MQ=255)
                                                                  tgATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGGGCCTGGCGCGATTTATCGGGGTTCTTACCAGGATTAATACGGAAcgcc                        >  2:6593341/1‑93 (MQ=255)
                                                                  tgATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAcgcc                        >  2:1592870/1‑93 (MQ=255)
                                                                   gATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAcgccg                       >  2:3147172/1‑93 (MQ=255)
                                                                    aTACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAcgccgc                      >  1:6639452/1‑93 (MQ=255)
                                                                    aTACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAcgccgc                      <  2:834723/93‑1 (MQ=255)
                                                                     tACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACgccgcc                     >  1:124462/1‑93 (MQ=255)
                                                                      aCTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCca                    <  2:124462/93‑1 (MQ=255)
                                                                       cTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCcag                   <  2:7933204/93‑1 (MQ=255)
                                                                         tCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCcagca                 <  2:3612222/93‑1 (MQ=255)
                                                                           ggCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGa               <  2:9107613/93‑1 (MQ=255)
                                                                           ggCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGa               <  2:351524/93‑1 (MQ=255)
                                                                            gcgcAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGAt              <  1:5432350/93‑1 (MQ=255)
                                                                            gcgcAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGAt              <  2:4903051/93‑1 (MQ=255)
                                                                            gcgcAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGAt              <  1:4782799/93‑1 (MQ=255)
                                                                             cgcAAAGAGGATACATACGCAAAACTCATCCAGCGACTGGCGAGATAAATCGGCGTACATAACAAGATTAATACCGAGCGCCGCCAGCAGATc             <  2:9250767/93‑1 (MQ=255)
                                                                             cgcAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATc             <  2:8556896/93‑1 (MQ=255)
                                                                             cgcAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATc             >  1:4096098/1‑93 (MQ=255)
                                                                              gcAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCg            <  2:2062761/93‑1 (MQ=255)
                                                                                  agagGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACg        >  1:10035120/1‑93 (MQ=255)
                                                                                     ggAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAAc     <  1:9839617/93‑1 (MQ=255)
                                                                                           |                                                                                         
AGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAG  >  NC_000913/1318282‑1318462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATT  >  NC_000913/1318280‑1318465
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CCAGGACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACG                                                                                              <  SRR4302540.9598382/94‑1 (MQ=60)
 CAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGT                                                                                             <  SRR4302540.3917624/94‑1 (MQ=60)
 CAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGT                                                                                             <  SRR4302540.8000545/94‑1 (MQ=60)
  AGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTA                                                                                            >  SRR4302540.1411687/1‑94 (MQ=60)
   GCACATTGATCAGGTGGCGGGTGTTCAGTTGCTGGCGAACCGGCATCGCGTGGCTGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTAC                                                                                           <  SRR4302540.1824924/94‑1 (MQ=60)
   GCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTAC                                                                                           <  SRR4302540.3886849/94‑1 (MQ=60)
    CACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACA                                                                                          <  SRR4302540.774385/94‑1 (MQ=60)
    CACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGGGTGATACTTCGGCGCAAAGAGGAAACGTACA                                                                                          >  SRR4302540.2549724/1‑94 (MQ=60)
     ACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACAC                                                                                         <  SRR4302540.6218373/94‑1 (MQ=60)
      CATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACC                                                                                        <  SRR4302540.3847391/94‑1 (MQ=60)
      CATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACC                                                                                        <  SRR4302540.7096554/94‑1 (MQ=60)
       ATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCT                                                                                       >  SRR4302540.4227028/1‑94 (MQ=60)
       ATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTAAACCT                                                                                       >  SRR4302540.3474989/1‑94 (MQ=60)
        TTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTA                                                                                      >  SRR4302540.8481902/1‑94 (MQ=60)
          GAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAAC                                                                                    <  SRR4302540.278015/94‑1 (MQ=60)
           AACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACT                                                                                   >  SRR4302540.27607/1‑94 (MQ=60)
             CAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCA                                                                                 <  SRR4302540.9367650/94‑1 (MQ=60)
             CAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCA                                                                                 >  SRR4302540.9131880/1‑94 (MQ=60)
              AGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCAT                                                                                >  SRR4302540.5620835/1‑94 (MQ=60)
                 GTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCA                                                                             <  SRR4302540.8785968/94‑1 (MQ=60)
                 GGGCGGGTGTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCA                                                                             <  SRR4302540.10041603/94‑1 (MQ=60)
                  ggctGGTTTGCAGTTGCTGGCGAACCGGCATCGCGTGGCTGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAG                                                                            <  SRR4302540.542637/90‑1 (MQ=60)
                  TGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAG                                                                            >  SRR4302540.5207709/1‑94 (MQ=60)
                  GGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAG                                                                            <  SRR4302540.3058567/94‑1 (MQ=60)
                   GCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGC                                                                           <  SRR4302540.1184439/94‑1 (MQ=60)
                      GGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCC                                                                        >  SRR4302540.2332847/1‑94 (MQ=60)
                        TTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGCAACGTACACCTAACTCATCCAGCGCCTG                                                                      >  SRR4302540.519284/1‑94 (MQ=60)
                         TTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGG                                                                     <  SRR4302540.103365/94‑1 (MQ=60)
                         TTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGG                                                                     <  SRR4302540.4208013/94‑1 (MQ=60)
                         TTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGG                                                                     <  SRR4302540.2036258/94‑1 (MQ=60)
                           TCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCG                                                                   >  SRR4302540.5978980/1‑94 (MQ=60)
                               TTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGAT                                                               >  SRR4302540.8423381/1‑94 (MQ=60)
                                  GTGGCGAACCGGCATCGCGTGGCGGAATCCGGTATGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTA                                                            <  SRR4302540.4230539/94‑1 (MQ=60)
                                   TGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTAT                                                           <  SRR4302540.3400794/94‑1 (MQ=60)
                                    GGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCGTGGCGCGATTTATC                                                          <  SRR4302540.6790223/94‑1 (MQ=60)
                                    GGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATC                                                          <  SRR4302540.1948967/94‑1 (MQ=60)
                                    GGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATC                                                          >  SRR4302540.5350063/1‑94 (MQ=60)
                                       GAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGC                                                       <  SRR4302540.2296065/94‑1 (MQ=60)
                                       GAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGC                                                       >  SRR4302540.6146489/1‑94 (MQ=60)
                                        AACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCG                                                      >  SRR4302540.4748292/1‑94 (MQ=60)
                                        AACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCG                                                      <  SRR4302540.2227910/94‑1 (MQ=60)
                                         ACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGT                                                     >  SRR4302540.2626796/1‑94 (MQ=60)
                                         ACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGT                                                     >  SRR4302540.10154344/1‑94 (MQ=60)
                                         ACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGT                                                     <  SRR4302540.6889260/94‑1 (MQ=60)
                                         ACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGT                                                     >  SRR4302540.1054662/1‑94 (MQ=60)
                                           CGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTC                                                   >  SRR4302540.9721980/1‑94 (MQ=60)
                                            GGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCA                                                  >  SRR4302540.3173559/1‑94 (MQ=60)
                                            GGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCA                                                  >  SRR4302540.2766972/1‑94 (MQ=60)
                                             GCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCAT                                                 >  SRR4302540.3872818/1‑94 (MQ=60)
                                             GCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCAT                                                 <  SRR4302540.9884408/94‑1 (MQ=60)
                                              CATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATA                                                <  SRR4302540.9771465/94‑1 (MQ=60)
                                               ATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATAT                                               <  SRR4302540.103210/94‑1 (MQ=60)
                                               ATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATAT                                               <  SRR4302540.10092623/94‑1 (MQ=60)
                                                TCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATC                                              <  SRR4302540.1323342/94‑1 (MQ=60)
                                                   CGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAG                                           >  SRR4302540.8294729/1‑94 (MQ=60)
                                                   CGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAG                                           <  SRR4302540.8977679/94‑1 (MQ=60)
                                                   CGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAG                                           >  SRR4302540.6735065/1‑94 (MQ=60)
                                                    GTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGA                                          >  SRR4302540.1340220/1‑94 (MQ=60)
                                                    GTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGA                                          >  SRR4302540.3465792/1‑94 (MQ=60)
                                                     gcgcgtagTCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATTGGCGTTCATATCAAGAT                                         <  SRR4302540.8178896/86‑1 (MQ=60)
                                                     TGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGAT                                         <  SRR4302540.8178898/94‑1 (MQ=60)
                                                      GGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATT                                        <  SRR4302540.5873861/94‑1 (MQ=60)
                                                      GGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATT                                        >  SRR4302540.8305073/1‑94 (MQ=60)
                                                      GGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATT                                        >  SRR4302540.8305072/1‑94 (MQ=60)
                                                       GCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTA                                       >  SRR4302540.345815/1‑94 (MQ=60)
                                                       GCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTA                                       <  SRR4302540.4154219/94‑1 (MQ=60)
                                                       GCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTA                                       <  SRR4302540.7080690/94‑1 (MQ=60)
                                                        CGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAA                                      <  SRR4302540.7059582/94‑1 (MQ=60)
                                                         GGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAAT                                     <  SRR4302540.4979358/94‑1 (MQ=60)
                                                         GGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAAT                                     <  SRR4302540.2095396/94‑1 (MQ=60)
                                                         GGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAAT                                     <  SRR4302540.1849945/94‑1 (MQ=60)
                                                            ATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACC                                  >  SRR4302540.5410171/1‑94 (MQ=60)
                                                            ATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACC                                  <  SRR4302540.1325195/94‑1 (MQ=60)
                                                               CGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAA                               <  SRR4302540.972136/94‑1 (MQ=60)
                                                               CGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAA                               >  SRR4302540.2398820/1‑94 (MQ=60)
                                                               CGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAA                               <  SRR4302540.8420943/94‑1 (MQ=60)
                                                               CGGTCTGATACTTCGGCGCAAAGAGGAACCGTACACCTAACTCATCCAGCGGCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAA                               <  SRR4302540.445188/94‑1 (MQ=60)
                                                                GGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAC                              >  SRR4302540.7779639/1‑94 (MQ=60)
                                                                GGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAC                              >  SRR4302540.2466288/1‑94 (MQ=60)
                                                                GGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAC                              <  SRR4302540.2093720/94‑1 (MQ=60)
                                                                GGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAC                              >  SRR4302540.6534086/1‑94 (MQ=60)
                                                                GGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAC                              <  SRR4302540.6505914/94‑1 (MQ=60)
                                                                GGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAC                              <  SRR4302540.1909429/94‑1 (MQ=60)
                                                                GGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAC                              <  SRR4302540.2628799/94‑1 (MQ=60)
                                                                 GTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACG                             >  SRR4302540.5240088/1‑94 (MQ=60)
                                                                 GTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACG                             <  SRR4302540.8585412/94‑1 (MQ=60)
                                                                  TGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTTATACCGAACGC                            >  SRR4302540.3981840/1‑94 (MQ=60)
                                                                   GTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCC                           >  SRR4302540.6185300/1‑94 (MQ=60)
                                                                     GATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGC                         >  SRR4302540.6712924/1‑94 (MQ=60)
                                                                      GTACTTCGGCGGAAAAAGGAAACGTACCCCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCC                        <  SRR4302540.2905626/94‑1 (MQ=60)
                                                                      ATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCC                        >  SRR4302540.125784/1‑94 (MQ=60)
                                                                       TACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCA                       >  SRR4302540.8983631/1‑94 (MQ=60)
                                                                       TACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCA                       >  SRR4302540.2256317/1‑94 (MQ=60)
                                                                         CTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGC                     >  SRR4302540.4081134/1‑94 (MQ=60)
                                                                          TTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCA                    >  SRR4302540.9744364/1‑94 (MQ=60)
                                                                           TCGGCGCAAAGAGGAAACGTACACCTAACTCATCAAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAG                   <  SRR4302540.4309831/94‑1 (MQ=60)
                                                                              GCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATC                >  SRR4302540.4140286/1‑94 (MQ=60)
                                                                              GCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATC                <  SRR4302540.5492096/94‑1 (MQ=60)
                                                                              GCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATC                >  SRR4302540.1269439/1‑94 (MQ=60)
                                                                              GCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATC                <  SRR4302540.5492097/94‑1 (MQ=60)
                                                                              GCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATC                <  SRR4302540.4833929/94‑1 (MQ=60)
                                                                               CGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCG               >  SRR4302540.1717987/1‑94 (MQ=60)
                                                                               CGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCG               >  SRR4302540.2554532/1‑94 (MQ=60)
                                                                                GCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGG              <  SRR4302540.2299312/94‑1 (MQ=60)
                                                                                GCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGG              <  SRR4302540.3531894/94‑1 (MQ=60)
                                                                                GCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGG              <  SRR4302540.9533825/94‑1 (MQ=60)
                                                                                GCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGG              >  SRR4302540.10011760/1‑94 (MQ=60)
                                                                                   AAGAGGAAACGTCCACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACG           <  SRR4302540.4387466/94‑1 (MQ=60)
                                                                                   AAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACG           >  SRR4302540.10151831/1‑94 (MQ=60)
                                                                                       GGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACC       <  SRR4302540.9954660/94‑1 (MQ=60)
                                                                                         AAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAG     >  SRR4302540.9135305/1‑94 (MQ=60)
                                                                                           ACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGAT   <  SRR4302540.4305566/94‑1 (MQ=60)
                                                                                            CGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATT  <  SRR4302540.2975303/94‑1 (MQ=60)
                                                                                             |                                                                                            
CCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATT  >  NC_000913/1318280‑1318465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑