Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,318,373 A→G C346R (TGT→CGT)  trpD ← anthranilate synthase component II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,318,3730AG100.0% 541.5 / NA 168C346R (TGT→CGT) trpDanthranilate synthase component II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (76/92);  total (76/92)

Only 100 of 176 total aligned reads displayed.

GCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAG  >  NC_000913/1318283‑1318462
                                                                                          |                                                                                         
gCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTa                                                                                         >  1:1304180/1‑93 (MQ=255)
gCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTa                                                                                         <  2:5835028/93‑1 (MQ=255)
gCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTa                                                                                         <  2:5688019/93‑1 (MQ=255)
  acaTTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTaca                                                                                       <  1:671501/93‑1 (MQ=255)
   caTTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTacac                                                                                      >  1:4657483/1‑93 (MQ=255)
    aTTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACAcc                                                                                     <  2:318547/93‑1 (MQ=255)
    aTTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACAcc                                                                                     >  1:2954535/1‑93 (MQ=255)
     ttGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCt                                                                                    <  1:3774750/93‑1 (MQ=255)
     ttGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCt                                                                                    <  1:4278731/93‑1 (MQ=255)
      tGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTa                                                                                   >  1:2961150/1‑93 (MQ=255)
      tGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTa                                                                                   <  2:5147130/93‑1 (MQ=255)
       gAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTaa                                                                                  <  1:5809623/93‑1 (MQ=255)
       gAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTaa                                                                                  <  2:6070612/93‑1 (MQ=255)
        aaCAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAAc                                                                                 <  2:1335982/93‑1 (MQ=255)
        aaCAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAAc                                                                                 <  1:475349/93‑1 (MQ=255)
         aCAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACt                                                                                >  1:882910/1‑93 (MQ=255)
           aGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCa                                                                              <  1:1562094/93‑1 (MQ=255)
                gCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAg                                                                         >  2:4930968/1‑93 (MQ=255)
                 cGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAgc                                                                        <  2:5498776/93‑1 (MQ=255)
                 cGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAgc                                                                        <  2:4405607/93‑1 (MQ=255)
                    gTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGcc                                                                     <  2:2635555/93‑1 (MQ=255)
                    gTTTTCAGTTGCTGGCGAACCGCCATCGCGTGTCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGcc                                                                     <  2:1755335/93‑1 (MQ=255)
                     ttttCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCt                                                                    <  1:1065759/93‑1 (MQ=255)
                     ttttCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCt                                                                    <  1:6252305/93‑1 (MQ=255)
                     gtttCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCt                                                                    >  2:3103171/2‑93 (MQ=255)
                      tttCAGTTGCTGGCGAACCGGCATCGCGCGGCGGAATCCCGGGCGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCCTCCAGCGCCTg                                                                   >  2:1287075/1‑93 (MQ=255)
                       ttCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTgg                                                                  <  1:2604818/93‑1 (MQ=255)
                       ttCAGTTGCTGACGAGCCGGCATCGCGTGACGGAATCCGGTGTGATACTTCGGCGCAAAGAGAAAACGTACTCCTAACTCATCCAGCGCCTgg                                                                  <  2:3570854/93‑1 (MQ=255)
                        tCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGgc                                                                 >  1:5506744/1‑93 (MQ=255)
                          aGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCTCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGgcgc                                                               >  1:4242236/1‑93 (MQ=255)
                           gTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGcgcg                                                              >  1:3226811/1‑93 (MQ=255)
                           gTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGcgcg                                                              >  1:5234682/1‑93 (MQ=255)
                               cTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGAttt                                                          <  1:4546472/93‑1 (MQ=255)
                                tGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTa                                                         <  2:5929690/93‑1 (MQ=255)
                                tGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTa                                                         <  1:4954540/93‑1 (MQ=255)
                                 ggCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTAt                                                        <  2:4150956/93‑1 (MQ=255)
                                 ggCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTAt                                                        <  2:1673282/93‑1 (MQ=255)
                                 ggCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTAt                                                        <  1:5364130/93‑1 (MQ=255)
                                  gCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAGCGTCCGCCTATCTCATCCAGCGCGTGGCGCGATTTATc                                                       <  2:2048406/93‑1 (MQ=255)
                                  gCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATc                                                       <  2:4698565/93‑1 (MQ=255)
                                  gCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATc                                                       <  1:2767209/93‑1 (MQ=255)
                                  gCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATc                                                       <  2:4252242/93‑1 (MQ=255)
                                  gCGAACCGGCATCGCGGGGCGTAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATc                                                       <  2:5908798/93‑1 (MQ=255)
                                   cGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCg                                                      <  1:3784974/93‑1 (MQ=255)
                                     aaCCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGc                                                    >  1:1402167/1‑93 (MQ=255)
                                      aCCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCg                                                   >  2:1407335/1‑93 (MQ=255)
                                      aCCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCg                                                   >  1:709031/1‑93 (MQ=255)
                                      aCCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCg                                                   >  1:3845187/1‑93 (MQ=255)
                                          gCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCa                                               <  2:443140/93‑1 (MQ=255)
                                          gCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCa                                               >  2:695966/1‑93 (MQ=255)
                                            aTCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCata                                             <  1:767179/93‑1 (MQ=255)
                                            aTCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCata                                             <  2:2185944/93‑1 (MQ=255)
                                             tCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCatat                                            <  1:4754138/93‑1 (MQ=255)
                                                 gTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAATCGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAg                                        <  1:166936/93‑1 (MQ=255)
                                                 gTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAg                                        >  2:5571958/1‑93 (MQ=255)
                                                  tGGCGGAATCCGGTGTGATATTTCGGCGCAAAGAGGAAGCGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGa                                       <  1:5882354/93‑1 (MQ=255)
                                                  tGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGa                                       <  2:3671578/93‑1 (MQ=255)
                                                  tGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGa                                       >  2:2476947/1‑93 (MQ=255)
                                                  gggCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGa                                       <  1:3461445/92‑1 (MQ=255)
                                                    gCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGAtt                                     <  1:2620634/93‑1 (MQ=255)
                                                     cGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTa                                    >  1:1951443/1‑93 (MQ=255)
                                                      ggAATCCGGTTTGATACTTCGGCGCAAAGCTGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTAATCGGCGTTCCTATCAAGAATaa                                   >  2:1605425/1‑93 (MQ=255)
                                                      ggAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTaa                                   >  2:2773024/1‑93 (MQ=255)
                                                       gAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCCGCGTTCATATCAAGATTAAt                                  >  2:5442987/1‑93 (MQ=255)
                                                        aaTCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGGTTTATCGGCGTTCATATCAAGACTAATa                                 >  2:1107001/1‑93 (MQ=255)
                                                         aTCCGGTGTAATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATAc                                <  2:234984/93‑1 (MQ=255)
                                                          tCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATAcc                               >  2:1743036/1‑93 (MQ=255)
                                                             ggTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGaa                            >  2:505969/1‑93 (MQ=255)
                                                               tgtgATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAcg                          >  1:3982596/1‑93 (MQ=255)
                                                                 tgATCCTTCGGCGCAAAGAGGAAACGTACCCCTACCTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATCCCGAAcgcc                        <  1:2865262/93‑1 (MQ=255)
                                                                 tgATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAcgcc                        >  2:4951462/1‑93 (MQ=255)
                                                                 tgATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAcgcc                        <  1:4302019/93‑1 (MQ=255)
                                                                  gATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAAcgccg                       >  1:2601758/1‑93 (MQ=255)
                                                                    tACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATGCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACgccgcc                     >  1:1003655/1‑93 (MQ=255)
                                                                    tACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACgccgcc                     <  2:5325410/93‑1 (MQ=255)
                                                                     aCTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCca                    <  1:2405474/93‑1 (MQ=255)
                                                                      cTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCcag                   >  1:1834001/1‑93 (MQ=255)
                                                                       ttCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCcagc                  <  1:6117066/93‑1 (MQ=255)
                                                                        tCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCcagca                 >  1:1813479/1‑93 (MQ=255)
                                                                        tCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCcagca                 >  2:3794821/1‑93 (MQ=255)
                                                                          ggCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGa               >  2:3213144/1‑93 (MQ=255)
                                                                          ggCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGa               >  2:3007492/1‑93 (MQ=255)
                                                                            cgcAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATc             <  1:606207/93‑1 (MQ=255)
                                                                             gcAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCg            >  2:1085946/1‑93 (MQ=255)
                                                                             gcAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCg            <  2:6184465/93‑1 (MQ=255)
                                                                             gcAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCg            >  2:4669737/1‑93 (MQ=255)
                                                                             gcAAAGAGGAAACGTACACCTAACGCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCg            <  2:1846510/93‑1 (MQ=255)
                                                                              cAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCgg           <  1:3567956/93‑1 (MQ=255)
                                                                               aaaGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGa          >  2:3925951/1‑93 (MQ=255)
                                                                               aaaGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGa          <  1:4921136/93‑1 (MQ=255)
                                                                               aaaGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGa          >  2:4870082/1‑93 (MQ=255)
                                                                               aaaGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGa          <  2:4032471/93‑1 (MQ=255)
                                                                               aaaGAGGAAACGTACACCTAACTCATCCAGAGTCAGGCGGCATCTACCGAGGTTCACACCAATACCAATCCCGACGCCCGCCAGCAGATCTGa          <  2:5254207/93‑1 (MQ=255)
                                                                                aaGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGAc         >  1:5148268/1‑93 (MQ=255)
                                                                                aaGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGAc         >  1:2256018/1‑93 (MQ=255)
                                                                                aaGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGAc         <  2:1280511/93‑1 (MQ=255)
                                                                                   agGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGaa      >  1:3066771/1‑93 (MQ=255)
                                                                                   agGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGaa      <  2:4022693/93‑1 (MQ=255)
                                                                                      aaaCGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTCATACCGAACGCCGCCAGCAGATCGGACGAAACa   >  1:3142384/1‑93 (MQ=255)
                                                                                       aaCGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAg  >  1:3498038/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAG  >  NC_000913/1318283‑1318462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATT  >  NC_000913/1318280‑1318465
                                                                                             |                                                                                            
CCAGCATATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACG                                                                                              >  SRR4302533.3474520/1‑94 (MQ=60)
CCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACG                                                                                              <  SRR4302533.4376689/94‑1 (MQ=60)
CCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACG                                                                                              >  SRR4302533.5793778/1‑94 (MQ=60)
 CAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGT                                                                                             <  SRR4302533.2546126/94‑1 (MQ=60)
 CAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGT                                                                                             <  SRR4302533.4851308/94‑1 (MQ=60)
  AGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCCAAGAGGACACGTA                                                                                            >  SRR4302533.2619882/1‑94 (MQ=60)
  AGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTA                                                                                            >  SRR4302533.1312018/1‑94 (MQ=60)
     ACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACAC                                                                                         <  SRR4302533.675467/94‑1 (MQ=60)
     ACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACAC                                                                                         <  SRR4302533.4003230/94‑1 (MQ=60)
     ACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACAC                                                                                         >  SRR4302533.4685706/1‑94 (MQ=60)
      CATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACC                                                                                        >  SRR4302533.2972603/1‑94 (MQ=60)
        TTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTA                                                                                      >  SRR4302533.4741542/1‑94 (MQ=60)
        TTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTA                                                                                      >  SRR4302533.2979253/1‑94 (MQ=60)
        TTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTA                                                                                      <  SRR4302533.3797888/94‑1 (MQ=60)
        TTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTA                                                                                      <  SRR4302533.4304462/94‑1 (MQ=60)
          GAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAAC                                                                                    >  SRR4302533.1448166/1‑94 (MQ=60)
          GAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAAC                                                                                    <  SRR4302533.5844707/94‑1 (MQ=60)
           AACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACT                                                                                   >  SRR4302533.888096/1‑94 (MQ=60)
           AACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACT                                                                                   <  SRR4302533.478186/94‑1 (MQ=60)
            ACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTC                                                                                  <  SRR4302533.4295895/94‑1 (MQ=60)
              AGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCAT                                                                                <  SRR4302533.5796291/94‑1 (MQ=60)
              AGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCAT                                                                                <  SRR4302533.1571497/94‑1 (MQ=60)
              AGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCAT                                                                                <  SRR4302533.128219/94‑1 (MQ=60)
                GGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCC                                                                              <  SRR4302533.4215175/94‑1 (MQ=60)
                  TGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAG                                                                            <  SRR4302533.738871/94‑1 (MQ=60)
                   GCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGC                                                                           <  SRR4302533.1875039/94‑1 (MQ=60)
                    CGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCG                                                                          <  SRR4302533.4415190/94‑1 (MQ=60)
                     GGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGC                                                                         >  SRR4302533.3485523/1‑94 (MQ=60)
                      GGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCC                                                                        >  SRR4302533.6194351/1‑94 (MQ=60)
                      GGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCC                                                                        <  SRR4302533.2786800/94‑1 (MQ=60)
                        TTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTG                                                                      <  SRR4302533.6289704/94‑1 (MQ=60)
                        TTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTG                                                                      <  SRR4302533.1072019/94‑1 (MQ=60)
                          TTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGC                                                                    >  SRR4302533.5539974/1‑94 (MQ=60)
                          TTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGC                                                                    >  SRR4302533.3213097/1‑94 (MQ=60)
                          TTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGC                                                                    <  SRR4302533.2620820/94‑1 (MQ=60)
                           TCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCG                                                                   <  SRR4302533.2328753/94‑1 (MQ=60)
                            CAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCTCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGC                                                                  >  SRR4302533.4267851/1‑94 (MQ=60)
                             AGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCG                                                                 >  SRR4302533.6122837/1‑94 (MQ=60)
                             AGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCG                                                                 >  SRR4302533.5266160/1‑94 (MQ=60)
                             AGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCG                                                                 >  SRR4302533.2669924/1‑94 (MQ=60)
                             AGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCG                                                                 >  SRR4302533.3246704/1‑94 (MQ=60)
                                 GCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTT                                                             <  SRR4302533.3808079/94‑1 (MQ=60)
                                  CTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTA                                                            <  SRR4302533.4574006/94‑1 (MQ=60)
                                   TGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTAT                                                           <  SRR4302533.4984551/94‑1 (MQ=60)
                                   TGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTAT                                                           <  SRR4302533.4984552/94‑1 (MQ=60)
                                    GGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATC                                                          <  SRR4302533.5396352/94‑1 (MQ=60)
                                     GCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCG                                                         <  SRR4302533.2784184/94‑1 (MQ=60)
                                      CGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGG                                                        <  SRR4302533.3808148/94‑1 (MQ=60)
                                       GAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGC                                                       >  SRR4302533.1410623/1‑94 (MQ=60)
                                       GAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGC                                                       >  SRR4302533.4861960/1‑94 (MQ=60)
                                        AACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCG                                                      >  SRR4302533.5494704/1‑94 (MQ=60)
                                        AACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCG                                                      >  SRR4302533.3868672/1‑94 (MQ=60)
                                        AACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCG                                                      >  SRR4302533.713219/1‑94 (MQ=60)
                                            GGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCA                                                  >  SRR4302533.1833814/1‑94 (MQ=60)
                                             GCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCAT                                                 <  SRR4302533.4574296/94‑1 (MQ=60)
                                               ATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATAT                                               <  SRR4302533.5391788/94‑1 (MQ=60)
                                               ATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATAT                                               <  SRR4302533.771704/94‑1 (MQ=60)
                                                TCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATC                                              <  SRR4302533.4782956/94‑1 (MQ=60)
                                                    GTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAATCGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGA                                          <  SRR4302533.167995/94‑1 (MQ=60)
                                                     TGGCGGAATCCGGTGTGATATTTCGGCGCAAAGAGGAAGCGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGAT                                         <  SRR4302533.5917903/94‑1 (MQ=60)
                                                     TGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGAT                                         <  SRR4302533.6036980/94‑1 (MQ=60)
                                                     GGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGAT                                         <  SRR4302533.3482810/94‑1 (MQ=60)
                                                     AGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACCCCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGAT                                         <  SRR4302533.4977688/94‑1 (MQ=60)
                                                       GCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTA                                       <  SRR4302533.2636720/94‑1 (MQ=60)
                                                       GCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTA                                       >  SRR4302533.1963335/1‑94 (MQ=60)
                                                         GGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAAT                                     >  SRR4302533.1341405/1‑94 (MQ=60)
                                                         GGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAAT                                     <  SRR4302533.3553501/94‑1 (MQ=60)
                                                           AATCCGGTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATAC                                   <  SRR4302533.3646385/94‑1 (MQ=60)
                                                                 GTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACG                             <  SRR4302533.2420993/94‑1 (MQ=60)
                                                                 GTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACG                             >  SRR4302533.4006842/1‑94 (MQ=60)
                                                                 GTGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACG                             >  SRR4302533.314364/1‑94 (MQ=60)
                                                                  TGTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGC                            <  SRR4302533.2944383/94‑1 (MQ=60)
                                                                   GTGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCC                           >  SRR4302533.695210/1‑94 (MQ=60)
                                                                    TGATCCTTCGGCGCAAAGAGGAAACGTACCCCTACCTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATCCCGAACGCCG                          <  SRR4302533.2882891/94‑1 (MQ=60)
                                                                    TGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCG                          <  SRR4302533.4327898/94‑1 (MQ=60)
                                                                    TGATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCG                          >  SRR4302533.2617745/1‑94 (MQ=60)
                                                                      ATACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATGCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCC                        >  SRR4302533.1009522/1‑94 (MQ=60)
                                                                       TACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCA                       <  SRR4302533.5051619/94‑1 (MQ=60)
                                                                        ACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAG                      >  SRR4302533.1845196/1‑94 (MQ=60)
                                                                        ACTTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAG                      <  SRR4302533.2420332/94‑1 (MQ=60)
                                                                          TTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCA                    >  SRR4302533.1824552/1‑94 (MQ=60)
                                                                          TTCGGCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCA                    <  SRR4302533.6153966/94‑1 (MQ=60)
                                                                              GCGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATC                <  SRR4302533.1756417/94‑1 (MQ=60)
                                                                               CGCAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCG               <  SRR4302533.609775/94‑1 (MQ=60)
                                                                                 CAAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGA             <  SRR4302533.3589944/94‑1 (MQ=60)
                                                                                  AAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGAC            >  SRR4302533.2269976/1‑94 (MQ=60)
                                                                                  AAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGAC            >  SRR4302533.5179374/1‑94 (MQ=60)
                                                                                  AAAGAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGAC            <  SRR4302533.4950947/94‑1 (MQ=60)
                                                                                  AAAGAGGAAACGCACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGAC            >  SRR4302533.304788/1‑94 (MQ=60)
                                                                                     GAGGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAA         >  SRR4302533.3085608/1‑94 (MQ=60)
                                                                                       GGAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACC       <  SRR4302533.826150/94‑1 (MQ=60)
                                                                                        GAAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTCATACCGAACGCCGCCAGCAGATCGGACGAAACA      >  SRR4302533.3161700/1‑94 (MQ=60)
                                                                                         AAACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAG     >  SRR4302533.3519609/1‑94 (MQ=60)
                                                                                           ACGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGAT   <  SRR4302533.2559051/94‑1 (MQ=60)
                                                                                            CGTACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATT  >  SRR4302533.3809076/1‑94 (MQ=60)
                                                                                             |                                                                                            
CCAGCACATTGAACAGGGTGCGGGTTTTCAGTTGCTGGCGAACCGGCATCGCGTGGCGGAATCCGGTGTGATACTTCGGCGCAAAGAGGAAACATACACCTAACTCATCCAGCGCCTGGCGCGATTTATCGGCGTTCATATCAAGATTAATACCGAACGCCGCCAGCAGATCGGACGAACCAGATT  >  NC_000913/1318280‑1318465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑