Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,320,179 C→T V264V (GTG→GTA)  trpE ← anthranilate synthase component I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,320,1790CT94.8% 810.4 / 15.6 248V264V (GTG→GTA) trpEanthranilate synthase component I
Reads supporting (aligned to +/- strand):  ref base C (6/7);  new base T (124/111);  total (130/118)
Fisher's exact test for biased strand distribution p-value = 7.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 274 total aligned reads displayed.

AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCA  >  NC_000913/1320089‑1320270
                                                                                          |                                                                                           
aaaCATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGcactac                                                                                           >  2:4704561/1‑93 (MQ=255)
 aaCATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCactacc                                                                                          >  1:3218977/1‑93 (MQ=255)
  aCATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCt                                                                                         <  1:1058781/93‑1 (MQ=255)
   cATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTg                                                                                        <  2:7547008/93‑1 (MQ=255)
    aTGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGTCAGAGAGAAACGGCGAGATGGCACTACCTgg                                                                                       <  2:3481149/93‑1 (MQ=255)
    aTGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTgg                                                                                       >  1:4977448/1‑93 (MQ=255)
    aTGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTgg                                                                                       >  2:3782175/1‑93 (MQ=255)
     tgtgCGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGa                                                                                      <  1:4453551/93‑1 (MQ=255)
     tGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATCGGCCGCCAGCGGTTACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGa                                                                                      >  2:3461474/1‑93 (MQ=255)
     tGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGa                                                                                      >  1:8605709/1‑93 (MQ=255)
      gTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGaa                                                                                     >  2:6764196/1‑93 (MQ=255)
      gTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGaa                                                                                     <  2:4286839/93‑1 (MQ=255)
       tACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGaaa                                                                                    <  2:1406030/93‑1 (MQ=255)
          gggCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAAtt                                                                                 >  1:2304682/1‑93 (MQ=255)
           ggCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAAttt                                                                                >  2:7933124/1‑93 (MQ=255)
            gCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTtc                                                                               >  1:6593926/1‑93 (MQ=255)
              tGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTtctc                                                                             <  2:4571848/93‑1 (MQ=255)
              tGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTtctc                                                                             <  2:7699045/93‑1 (MQ=255)
               gggATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTcc                                                                            <  1:4364547/93‑1 (MQ=255)
                ggATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCa                                                                           >  2:4535049/1‑93 (MQ=255)
                ggATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCa                                                                           <  2:8192723/93‑1 (MQ=255)
                 gATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAg                                                                          >  1:8915208/1‑93 (MQ=255)
                  aTTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAgc                                                                         <  1:10035927/93‑1 (MQ=255)
                   ttACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAgcg                                                                        <  1:9697107/93‑1 (MQ=255)
                   ttACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAgcg                                                                        >  1:5685948/1‑93 (MQ=255)
                   ttACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAgcg                                                                        >  1:8818342/1‑93 (MQ=255)
                   ttACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAgcg                                                                        >  1:5312817/1‑93 (MQ=255)
                    tACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAgcgc                                                                       >  2:196789/1‑93 (MQ=255)
                    tACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAgcgc                                                                       >  2:6100507/1‑93 (MQ=255)
                      ctctTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGa                                                                     >  1:6019061/1‑93 (MQ=255)
                       tctTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGaa                                                                    >  1:9803245/1‑93 (MQ=255)
                       tctTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGaa                                                                    <  2:2458555/93‑1 (MQ=255)
                         tttttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATc                                                                  <  2:7734882/93‑1 (MQ=255)
                         tttttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATc                                                                  <  1:4047099/93‑1 (MQ=255)
                         tttttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATc                                                                  <  1:3777220/93‑1 (MQ=255)
                          ttttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCg                                                                 >  2:1882761/1‑93 (MQ=255)
                          ttttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCg                                                                 >  1:7926024/1‑93 (MQ=255)
                          ttttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCg                                                                 >  2:9596117/1‑93 (MQ=255)
                             tCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCtt                                                              >  1:8044556/1‑93 (MQ=255)
                              cAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCttt                                                             <  2:7064867/93‑1 (MQ=255)
                              cAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCttt                                                             >  2:1052546/1‑93 (MQ=255)
                                gCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCttttt                                                           <  2:5186261/93‑1 (MQ=255)
                                gCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCttttt                                                           <  2:3918783/93‑1 (MQ=255)
                                gCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCttttt                                                           <  2:3563594/93‑1 (MQ=255)
                                       aTAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGcaacaa                                                    >  2:7239434/1‑93 (MQ=255)
                                        tAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACaaa                                                   >  2:5734651/1‑93 (MQ=255)
                                         aGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAAc                                                  <  1:1229985/93‑1 (MQ=255)
                                          ggCCGCCAGCGGTGACGGGCAGGGCAGAGCGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACg                                                 >  2:5189436/1‑93 (MQ=255)
                                           gccgccAGCGGTGACGGGCAGGGAAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGc                                                <  2:5613138/93‑1 (MQ=255)
                                             cgccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCAc                                              <  1:421838/93‑1 (MQ=255)
                                             cgccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCAc                                              <  1:1548065/93‑1 (MQ=255)
                                             cgccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCAc                                              <  1:4998859/93‑1 (MQ=255)
                                             cgccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCAc                                              <  1:6066249/93‑1 (MQ=255)
                                              gccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACt                                             <  1:1300145/93‑1 (MQ=255)
                                              gccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACt                                             <  1:2527996/93‑1 (MQ=255)
                                               ccAGCGGTGACGGGCAGGGCAGAGATAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTa                                            <  1:6915576/93‑1 (MQ=255)
                                               ccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTa                                            <  2:8647946/93‑1 (MQ=255)
                                               ccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTa                                            <  1:8253472/93‑1 (MQ=255)
                                                 aGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACg                                          >  1:2449221/1‑93 (MQ=255)
                                                  gCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGc                                         <  2:7476603/93‑1 (MQ=255)
                                                  gCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGc                                         >  2:3094542/1‑93 (MQ=255)
                                                    ggTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGccc                                       >  1:7277236/1‑92 (MQ=255)
                                                     gTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAc                                      <  2:7427990/93‑1 (MQ=255)
                                                     gTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAc                                      >  2:9607921/1‑93 (MQ=255)
                                                     gTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAc                                      >  2:9365287/1‑93 (MQ=255)
                                                     gTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAc                                      <  2:8704047/93‑1 (MQ=255)
                                                         cGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGaa                                  <  1:9818930/93‑1 (MQ=255)
                                                          gggcagggcaGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAAc                                 <  2:2267015/93‑1 (MQ=255)
                                                          gggcagggcaGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAAc                                 <  1:7855141/93‑1 (MQ=255)
                                                              agggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCtt                             >  2:6877456/1‑93 (MQ=255)
                                                              agggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCtt                             >  2:1403975/1‑93 (MQ=255)
                                                               gggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTc                            >  2:1694031/1‑93 (MQ=255)
                                                               gggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTc                            <  1:5844559/93‑1 (MQ=255)
                                                               gggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTc                            <  1:6279234/93‑1 (MQ=255)
                                                               gggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTc                            >  1:7615926/1‑93 (MQ=255)
                                                                ggcagAGAGAAACGGCGAGATGGCACTACCTGGAAATTTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGAACTACGCCACCGAACACTTCa                           <  2:8937834/93‑1 (MQ=255)
                                                                ggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCa                           <  2:9827653/93‑1 (MQ=255)
                                                                ggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCa                           <  2:6617833/93‑1 (MQ=255)
                                                                 gcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGTGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCAt                          <  2:7510903/93‑1 (MQ=255)
                                                                 gcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCAt                          <  1:5215839/93‑1 (MQ=255)
                                                                    gagagaAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGc                       >  1:9088564/1‑93 (MQ=255)
                                                                    gagagaAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGc                       <  1:8298455/93‑1 (MQ=255)
                                                                      aagaAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTGTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGctc                     <  1:2039245/92‑1 (MQ=255)
                                                                       agaAGCGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGctct                    <  1:7523777/93‑1 (MQ=255)
                                                                       agaAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGctct                    >  1:7140825/1‑93 (MQ=255)
                                                                         aaaaGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGa                  >  2:989118/1‑93 (MQ=255)
                                                                          aaCGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGAt                 <  2:6338846/93‑1 (MQ=255)
                                                                           aCGGCGAGATGGCCCTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAGCGAACTCTTCATCGCTCTGAtt                <  1:6218893/93‑1 (MQ=255)
                                                                            cGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTa               <  1:3026419/93‑1 (MQ=255)
                                                                             ggCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTAc              >  2:6530841/1‑93 (MQ=255)
                                                                              gCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACa             >  2:6149284/1‑93 (MQ=255)
                                                                                gagaTGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACAtt           >  2:4906716/1‑93 (MQ=255)
                                                                                  gaTGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTca         <  1:9958759/93‑1 (MQ=255)
                                                                                   aTGGCACTACCTGGAAAATTTCTCCAGCGCGTATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTcac        <  1:8393876/93‑1 (MQ=255)
                                                                                    tGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTcaca       <  1:6037480/93‑1 (MQ=255)
                                                                                    tGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTcaca       <  2:5919782/93‑1 (MQ=255)
                                                                                      gCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAAc     <  1:2381425/93‑1 (MQ=255)
                                                                                       cactacCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACg    <  1:8736548/93‑1 (MQ=255)
                                                                                       caccacCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACg    >  2:8421583/1‑93 (MQ=255)
                                                                                         ccaccTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCa  >  2:3070717/1‑93 (MQ=255)
                                                                                          |                                                                                           
AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCA  >  NC_000913/1320089‑1320270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATA  >  NC_000913/1320089‑1320272
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AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAGACGGCGAGAGGGCACTACC                                                                                            >  SRR4302540.7664167/1‑94 (MQ=60)
AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACC                                                                                            <  SRR4302540.9908564/94‑1 (MQ=60)
AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACC                                                                                            >  SRR4302540.7664172/1‑94 (MQ=60)
AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACC                                                                                            >  SRR4302540.3153019/1‑94 (MQ=60)
AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACC                                                                                            >  SRR4302540.3253944/1‑94 (MQ=60)
AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACC                                                                                            <  SRR4302540.7320432/94‑1 (MQ=60)
  ACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTG                                                                                          <  SRR4302540.1069561/94‑1 (MQ=60)
  ACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTG                                                                                          >  SRR4302540.6067182/1‑94 (MQ=60)
  ACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTG                                                                                          >  SRR4302540.9811999/1‑94 (MQ=60)
   CATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGG                                                                                         >  SRR4302540.6279609/1‑94 (MQ=60)
   CATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGG                                                                                         >  SRR4302540.5031325/1‑94 (MQ=60)
   CATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGG                                                                                         >  SRR4302540.6279898/1‑94 (MQ=60)
    ATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGA                                                                                        >  SRR4302540.8705708/1‑94 (MQ=60)
     TGTGCGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAA                                                                                       <  SRR4302540.4501829/94‑1 (MQ=60)
     TGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGCCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAA                                                                                       <  SRR4302540.4501831/94‑1 (MQ=60)
      GTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAA                                                                                      >  SRR4302540.234921/1‑94 (MQ=60)
       TACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAA                                                                                     >  SRR4302540.8108416/1‑94 (MQ=60)
        ACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAAT                                                                                    <  SRR4302540.2681886/94‑1 (MQ=60)
         CGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATT                                                                                   >  SRR4302540.2329481/1‑94 (MQ=60)
          GGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTT                                                                                  >  SRR4302540.2433494/1‑94 (MQ=60)
          GGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTT                                                                                  >  SRR4302540.3214888/1‑94 (MQ=60)
           GGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTC                                                                                 >  SRR4302540.6666951/1‑94 (MQ=60)
           GGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTC                                                                                 <  SRR4302540.5192215/94‑1 (MQ=60)
            GCTGGGATTCCTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGCCACTACATGGAAAATTTCT                                                                                >  SRR4302540.2798087/1‑94 (MQ=60)
            GCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGACACGGCGAGATGGCACTACCTGGAAAATTTCT                                                                                >  SRR4302540.5763962/1‑94 (MQ=60)
            GCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCT                                                                                >  SRR4302540.8495998/1‑94 (MQ=60)
            GCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCT                                                                                <  SRR4302540.8923549/94‑1 (MQ=60)
            GCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCT                                                                                >  SRR4302540.10094653/1‑94 (MQ=60)
              TGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCC                                                                              <  SRR4302540.5046047/94‑1 (MQ=60)
               GGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCA                                                                             <  SRR4302540.4411987/94‑1 (MQ=60)
                GGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAG                                                                            >  SRR4302540.9018453/1‑94 (MQ=60)
                GGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAG                                                                            <  SRR4302540.7532916/94‑1 (MQ=60)
                 GATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGC                                                                           >  SRR4302540.4259625/1‑94 (MQ=60)
                  ATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGTCACTACCTGGAAAATTTCTAAAGCG                                                                          >  SRR4302540.8920300/1‑94 (MQ=60)
                  ATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCG                                                                          >  SRR4302540.5748541/1‑94 (MQ=60)
                  ATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCG                                                                          >  SRR4302540.8920301/1‑94 (MQ=60)
                  ATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCG                                                                          <  SRR4302540.10152645/94‑1 (MQ=60)
                  ATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCG                                                                          >  SRR4302540.5370983/1‑94 (MQ=60)
                   TTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGC                                                                         <  SRR4302540.9811124/94‑1 (MQ=60)
                     ACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGA                                                                       >  SRR4302540.6085818/1‑94 (MQ=60)
                     ACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGA                                                                       >  SRR4302540.4111599/1‑94 (MQ=60)
                      CTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAA                                                                      >  SRR4302540.9918004/1‑94 (MQ=60)
                      CTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAA                                                                      >  SRR4302540.9156707/1‑94 (MQ=60)
                        CTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATC                                                                    <  SRR4302540.9718629/94‑1 (MQ=60)
                         TTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCG                                                                   <  SRR4302540.4090736/94‑1 (MQ=60)
                         TTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCG                                                                   <  SRR4302540.3818427/94‑1 (MQ=60)
                         TTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCG                                                                   >  SRR4302540.8018407/1‑94 (MQ=60)
                           TTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGGAGAGGGAAACGGAGAGATGGCACTACCTGGAAAATTTCTGCAGCGCGAATCGCT                                                                 >  SRR4302540.1846956/1‑94 (MQ=60)
                           TTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCT                                                                 >  SRR4302540.2542384/1‑94 (MQ=60)
                           TTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGAT                                                                 >  SRR4302540.2542376/1‑94 (MQ=60)
                            TTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTT                                                                >  SRR4302540.8138020/1‑94 (MQ=60)
                             TCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTT                                                               >  SRR4302540.2033584/1‑94 (MQ=60)
                                GCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTG                                                            >  SRR4302540.7847032/1‑94 (MQ=60)
                                 CACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGC                                                           >  SRR4302540.8110954/1‑94 (MQ=60)
                                    GTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAAC                                                        >  SRR4302540.1067391/1‑94 (MQ=60)
                                     gaaaAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACA                                                       <  SRR4302540.1540562/90‑1 (MQ=60)
                                     TAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACA                                                       <  SRR4302540.2380226/94‑1 (MQ=60)
                                      AATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAA                                                      >  SRR4302540.4341891/1‑94 (MQ=60)
                                       ATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAA                                                     >  SRR4302540.8827455/1‑94 (MQ=60)
                                       ATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAA                                                     >  SRR4302540.7894418/1‑94 (MQ=60)
                                       ATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAA                                                     >  SRR4302540.2133293/1‑94 (MQ=60)
                                        TAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAAC                                                    >  SRR4302540.1248799/1‑94 (MQ=60)
                                         AGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACG                                                   >  SRR4302540.9194178/1‑94 (MQ=60)
                                         AGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACG                                                   <  SRR4302540.1242555/94‑1 (MQ=60)
                                         AGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACG                                                   <  SRR4302540.4946195/94‑1 (MQ=60)
                                          GGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGC                                                  <  SRR4302540.4474559/94‑1 (MQ=60)
                                            CCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCAC                                                <  SRR4302540.5577957/94‑1 (MQ=60)
                                             CGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACT                                               <  SRR4302540.1565138/94‑1 (MQ=60)
                                             CGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACT                                               <  SRR4302540.426282/94‑1 (MQ=60)
                                             CGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACT                                               <  SRR4302540.6133695/94‑1 (MQ=60)
                                             CGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACT                                               <  SRR4302540.5052983/94‑1 (MQ=60)
                                              GCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTA                                              <  SRR4302540.2555276/94‑1 (MQ=60)
                                              GCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTA                                              <  SRR4302540.1313621/94‑1 (MQ=60)
                                              GCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTA                                              <  SRR4302540.1313622/94‑1 (MQ=60)
                                               GCAGCGGTGACAGGCAGGGAAGAGAGAAACGGCGAGATGGCACTGCCTGGAAAATTGCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTAC                                             <  SRR4302540.6653657/94‑1 (MQ=60)
                                               CCAGCGGTGACGGGCAGGGCAGAGATAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTAC                                             <  SRR4302540.6992529/94‑1 (MQ=60)
                                               CCAGCGGTGACGGGCAGGGCAGAGATAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTAC                                             <  SRR4302540.6992528/94‑1 (MQ=60)
                                               CCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTAC                                             <  SRR4302540.8349402/94‑1 (MQ=60)
                                               CCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTAC                                             >  SRR4302540.5709448/1‑94 (MQ=60)
                                               CCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTAC                                             <  SRR4302540.8333086/94‑1 (MQ=60)
                                               CCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTAC                                             <  SRR4302540.6653656/94‑1 (MQ=60)
                                                CAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACG                                            >  SRR4302540.6941249/1‑94 (MQ=60)
                                                CAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACG                                            >  SRR4302540.2475595/1‑94 (MQ=60)
                                                  GCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCC                                          >  SRR4302540.8814488/1‑94 (MQ=60)
                                                   CGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCC                                         >  SRR4302540.7358432/1‑94 (MQ=60)
                                                        ACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAA                                    >  SRR4302540.8380156/1‑94 (MQ=60)
                                                        ACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAA                                    <  SRR4302540.6088310/94‑1 (MQ=60)
                                                         CGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAAC                                   <  SRR4302540.9933814/94‑1 (MQ=60)
                                                         CGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAAC                                   <  SRR4302540.9933815/94‑1 (MQ=60)
                                                          GGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACT                                  <  SRR4302540.7946925/94‑1 (MQ=60)
                                                           GGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTC                                 >  SRR4302540.1786338/1‑94 (MQ=60)
                                                            GCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCT                                <  SRR4302540.9240666/94‑1 (MQ=60)
                                                              AGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTC                              >  SRR4302540.7701546/1‑94 (MQ=60)
                                                               GGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCA                             <  SRR4302540.5909208/94‑1 (MQ=60)
                                                               GGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCA                             >  SRR4302540.4295281/1‑94 (MQ=60)
                                                               GGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCA                             <  SRR4302540.6349110/94‑1 (MQ=60)
                                                                GGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCACCAAACGCACTACGCCACCGAACTCTTCAT                            <  SRR4302540.404789/94‑1 (MQ=60)
                                                                 GCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATC                           <  SRR4302540.6312320/94‑1 (MQ=60)
                                                                 GCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATC                           <  SRR4302540.5272688/94‑1 (MQ=60)
                                                                 GCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATC                           <  SRR4302540.882993/94‑1 (MQ=60)
                                                                  CAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCG                          >  SRR4302540.5044749/1‑94 (MQ=60)
                                                                  CAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCG                          >  SRR4302540.5069388/1‑94 (MQ=60)
                                                                  CAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCG                          >  SRR4302540.5801375/1‑94 (MQ=60)
                                                                   AGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGC                         >  SRR4302540.9197750/1‑94 (MQ=60)
                                                                   AGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGC                         >  SRR4302540.7744210/1‑94 (MQ=60)
                                                                    GAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCT                        <  SRR4302540.8394812/94‑1 (MQ=60)
                                                                    GAGAGAAACGGCGAGATGGCACCACCTGGAAAAGTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCT                        <  SRR4302540.8394813/94‑1 (MQ=60)
                                                                     AGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTC                       <  SRR4302540.9967584/94‑1 (MQ=60)
                                                                      GAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCT                      >  SRR4302540.7220377/1‑94 (MQ=60)
                                                                      AAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTGTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCT                      <  SRR4302540.2061223/94‑1 (MQ=60)
                                                                       AGAAGCGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTG                     <  SRR4302540.7607947/94‑1 (MQ=60)
                                                                        GAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGA                    >  SRR4302540.7178348/1‑94 (MQ=60)
                                                                         AAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGAT                   <  SRR4302540.8247780/94‑1 (MQ=60)
                                                                         AAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGAT                   >  SRR4302540.10025689/1‑94 (MQ=60)
                                                                         AAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGAT                   >  SRR4302540.8609521/1‑94 (MQ=60)
                                                                           ACGGCGAGATGGCCCTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAGCGAACTCTTCATCGCTCTGATTA                 <  SRR4302540.6288080/94‑1 (MQ=60)
                                                                            CGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTAC                <  SRR4302540.3059660/94‑1 (MQ=60)
                                                                            CGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTAC                >  SRR4302540.4016876/1‑94 (MQ=60)
                                                                               CGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATT             >  SRR4302540.2274159/1‑94 (MQ=60)
                                                                               CGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATT             >  SRR4302540.5282521/1‑94 (MQ=60)
                                                                                GAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTC            >  SRR4302540.798542/1‑94 (MQ=60)
                                                                                GAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTC            <  SRR4302540.7552033/94‑1 (MQ=60)
                                                                                GAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTA            >  SRR4302540.1171894/1‑94 (MQ=60)
                                                                                  GATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCAC          <  SRR4302540.10074981/94‑1 (MQ=60)
                                                                                   ATGGCACTACCTGGAAAATTTCTCCAGCGCGTATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACA         <  SRR4302540.8491284/94‑1 (MQ=60)
                                                                                    TGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAA        <  SRR4302540.6104508/94‑1 (MQ=60)
                                                                                    TGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAA        >  SRR4302540.6980870/1‑94 (MQ=60)
                                                                                    AGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAA        <  SRR4302540.5946273/94‑1 (MQ=60)
                                                                                     GGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAAC       <  SRR4302540.6117359/94‑1 (MQ=60)
                                                                                     GGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAAC       <  SRR4302540.1114189/94‑1 (MQ=60)
                                                                                     GGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTGTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGAGTACATTCACAAC       <  SRR4302540.6117358/94‑1 (MQ=60)
                                                                                      GCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACG      <  SRR4302540.8374720/94‑1 (MQ=60)
                                                                                      GCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACG      <  SRR4302540.3659830/94‑1 (MQ=60)
                                                                                      GCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACG      <  SRR4302540.2407080/94‑1 (MQ=60)
                                                                                       CACTACCTGGAAAATTTCTCCAGCGCGAATTGCTTTTTGCAACAAACGCACTACGCCACCGTACTCTTCATCGCTCTGATTAaattcttaacga     >  SRR4302540.7317144/1‑82 (MQ=60)
                                                                                       CACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGC     <  SRR4302540.8837984/94‑1 (MQ=60)
                                                                                        ACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCA    <  SRR4302540.9929755/94‑1 (MQ=60)
                                                                                         CTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCAT   <  SRR4302540.1785928/94‑1 (MQ=60)
                                                                                         CTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCAT   >  SRR4302540.5419243/1‑94 (MQ=60)
                                                                                         CTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCAT   <  SRR4302540.5708066/94‑1 (MQ=60)
                                                                                          TACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATA  <  SRR4302540.9431978/94‑1 (MQ=60)
                                                                                          |                                                                                             
AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATA  >  NC_000913/1320089‑1320272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑