Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,320,179 C→T V264V (GTG→GTA)  trpE ← anthranilate synthase component I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,320,1790CT100.0% 627.0 / NA 171V264V (GTG→GTA) trpEanthranilate synthase component I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (96/75);  total (96/75)

Only 100 of 177 total aligned reads displayed.

AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAAC  >  NC_000913/1320089‑1320267
                                                                                          |                                                                                        
  aCATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCt                                                                                      >  1:702716/1‑93 (MQ=255)
  aCATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCt                                                                                      <  1:2971553/93‑1 (MQ=255)
   cATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTg                                                                                     >  1:2101716/1‑93 (MQ=255)
   cATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTg                                                                                     >  1:3452584/1‑93 (MQ=255)
   cATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTg                                                                                     >  1:2108426/1‑93 (MQ=255)
   cATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTg                                                                                     >  1:641339/1‑93 (MQ=255)
    aTGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTgg                                                                                    >  1:5566090/1‑93 (MQ=255)
     tGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGa                                                                                   >  2:4329309/1‑93 (MQ=255)
     tGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGa                                                                                   <  2:5394750/93‑1 (MQ=255)
     tGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGa                                                                                   <  2:370626/93‑1 (MQ=255)
      gTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGaa                                                                                  >  1:2338309/1‑93 (MQ=255)
        aCGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGaaaa                                                                                <  1:4845473/93‑1 (MQ=255)
        aCGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGaaaa                                                                                <  1:955340/93‑1 (MQ=255)
        aCGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGaaaa                                                                                <  2:6164534/93‑1 (MQ=255)
          gggCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAAtt                                                                              >  2:2785024/1‑93 (MQ=255)
            gCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTtc                                                                            <  2:4003218/93‑1 (MQ=255)
            gCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTtc                                                                            >  1:3180077/1‑93 (MQ=255)
            gCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTtc                                                                            >  2:1738226/1‑93 (MQ=255)
              tGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTtctc                                                                          <  2:3812491/93‑1 (MQ=255)
               gggATTCCTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTcc                                                                         <  2:5002404/93‑1 (MQ=255)
                ggATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCa                                                                        >  1:670103/1‑93 (MQ=255)
                 gATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAg                                                                       <  1:6017070/93‑1 (MQ=255)
                 gATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAg                                                                       >  1:4197967/1‑93 (MQ=255)
                 gATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAg                                                                       >  1:610782/1‑93 (MQ=255)
                   ttACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAgcg                                                                     <  1:3589394/93‑1 (MQ=255)
                   ttACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAgcg                                                                     <  1:1828258/93‑1 (MQ=255)
                    tACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAgcgc                                                                    <  1:2012955/93‑1 (MQ=255)
                    tACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAgcgc                                                                    >  1:5957388/1‑93 (MQ=255)
                     aCTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGcgcg                                                                   >  1:3337700/1‑93 (MQ=255)
                     aCTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGcgcg                                                                   >  1:4820820/1‑93 (MQ=255)
                      ctctTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGa                                                                  >  1:3416789/1‑93 (MQ=255)
                       tctTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGaa                                                                 <  2:3987000/93‑1 (MQ=255)
                        ctTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAAt                                                                >  2:2589676/1‑93 (MQ=255)
                         tttttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATc                                                               >  2:5052916/1‑93 (MQ=255)
                         tttttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATc                                                               >  2:5663099/1‑93 (MQ=255)
                         tttttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATc                                                               >  1:5806355/1‑93 (MQ=255)
                          ttttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCg                                                              >  1:3581721/1‑93 (MQ=255)
                            ttCAGCACGTAATAGGCCGCCAGCNGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCt                                                            <  2:1088659/93‑1 (MQ=255)
                            ttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCt                                                            <  1:2623802/93‑1 (MQ=255)
                            ttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCt                                                            <  2:1797487/93‑1 (MQ=255)
                               aGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCtttt                                                         >  1:5186078/1‑93 (MQ=255)
                                gCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCttttt                                                        <  2:5765032/93‑1 (MQ=255)
                                  aCGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGc                                                      >  2:4671252/1‑93 (MQ=255)
                                   cGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGca                                                     >  1:1643342/1‑93 (MQ=255)
                                    gTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGcaa                                                    >  1:4511169/1‑93 (MQ=255)
                                     tAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGcaac                                                   >  2:1391901/1‑93 (MQ=255)
                                      aaTAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGcaaca                                                  <  2:5825091/93‑1 (MQ=255)
                                      aaTAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTAGAAAATTTCTCCAGCGCGAATCGCTTTTTGcaaca                                                  >  2:6260603/1‑93 (MQ=255)
                                             cgccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGATTCGCTTTTTGCAACAAACGCAc                                           <  2:3580922/93‑1 (MQ=255)
                                             cgccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCAc                                           <  1:4099570/93‑1 (MQ=255)
                                              gccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACt                                          <  2:1395750/93‑1 (MQ=255)
                                               ccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTa                                         >  2:3014695/1‑93 (MQ=255)
                                               ccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTa                                         <  1:332723/93‑1 (MQ=255)
                                                  gCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGc                                      >  1:4977751/1‑93 (MQ=255)
                                                  gCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGc                                      <  2:4455658/93‑1 (MQ=255)
                                                    ggTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCa                                    >  1:3613902/1‑93 (MQ=255)
                                                     gTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAc                                   >  2:3414026/1‑93 (MQ=255)
                                                     gTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAc                                   <  2:93027/93‑1 (MQ=255)
                                                     gTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAc                                   >  1:535901/1‑93 (MQ=255)
                                                      tGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAcc                                  <  1:4688492/93‑1 (MQ=255)
                                                      tGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAcc                                  >  1:5874748/1‑93 (MQ=255)
                                                       gACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCg                                 >  1:2853266/1‑93 (MQ=255)
                                                        aCGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGa                                <  1:146803/93‑1 (MQ=255)
                                                         cGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGaa                               >  2:1769309/1‑93 (MQ=255)
                                                          gggcagggcaGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAAc                              >  1:4788043/1‑93 (MQ=255)
                                                          gggcagggcaGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAAc                              <  1:1385349/93‑1 (MQ=255)
                                                           ggcagggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAAct                             >  1:5614539/1‑93 (MQ=255)
                                                            gcagggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAActc                            <  1:2926358/93‑1 (MQ=255)
                                                             cagggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTCCGCCACCGAActct                           <  2:4611952/93‑1 (MQ=255)
                                                             cagggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAActct                           <  1:5681514/93‑1 (MQ=255)
                                                              agggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGATCTCtt                          <  2:769497/93‑1 (MQ=255)
                                                              agggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCtt                          >  1:2633721/1‑93 (MQ=255)
                                                               gggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTc                         <  1:1315615/93‑1 (MQ=255)
                                                                ggcagAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCa                        <  2:4935786/93‑1 (MQ=255)
                                                                   agagagAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCg                     <  1:4446974/93‑1 (MQ=255)
                                                                   agagagAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCg                     <  1:1055066/93‑1 (MQ=255)
                                                                   agagagAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCg                     <  1:783844/93‑1 (MQ=255)
                                                                   agagagAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCg                     >  2:2449478/1‑93 (MQ=255)
                                                                    gagagaAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGc                    >  1:3897514/1‑93 (MQ=255)
                                                                      gagaAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGctc                  >  2:3475665/1‑93 (MQ=255)
                                                                        gaAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTg                >  2:3242594/1‑93 (MQ=255)
                                                                           aCGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGAtt             <  2:1666397/93‑1 (MQ=255)
                                                                             ggCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTAc           >  2:1927155/1‑93 (MQ=255)
                                                                             ggCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTAc           >  2:1550899/1‑93 (MQ=255)
                                                                               cGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACAt         >  1:5849441/1‑93 (MQ=255)
                                                                                gagaTGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACAtt        >  2:194393/1‑93 (MQ=255)
                                                                                 agaTGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTc       <  1:5479427/93‑1 (MQ=255)
                                                                                 agaTGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTc       >  2:3941148/1‑93 (MQ=255)
                                                                                 aaaTGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTc       <  1:1169634/91‑1 (MQ=255)
                                                                                  gaTGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTca      >  2:2721534/1‑93 (MQ=255)
                                                                                   aTGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTcac     <  2:5339779/93‑1 (MQ=255)
                                                                                    tGGCAGTACCTGGAAAATTCCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTcaca    <  1:4634888/93‑1 (MQ=255)
                                                                                    tGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTcaca    >  1:4933534/1‑93 (MQ=255)
                                                                                    tGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTcaca    >  2:1268514/1‑93 (MQ=255)
                                                                                    tGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTcaca    <  1:4671430/93‑1 (MQ=255)
                                                                                     ggCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACaa   <  2:57209/93‑1 (MQ=255)
                                                                                     ggCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACaa   <  2:5250201/93‑1 (MQ=255)
                                                                                      gCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAAc  <  1:5965429/93‑1 (MQ=255)
                                                                                      gCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAAc  <  1:3306533/93‑1 (MQ=255)
                                                                                      gCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTCATTACATTCACAAc  <  2:3847946/93‑1 (MQ=255)
                                                                                          |                                                                                        
AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAAC  >  NC_000913/1320089‑1320267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAAAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATA  >  NC_000913/1320086‑1320272
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AAAAAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACT                                                                                               >  SRR4302533.1671652/1‑94 (MQ=60)
AAAAAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACT                                                                                               >  SRR4302533.1662488/1‑94 (MQ=60)
 AAAAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGTGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTA                                                                                              >  SRR4302533.2694644/1‑94 (MQ=60)
   AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACC                                                                                            <  SRR4302533.3447252/94‑1 (MQ=60)
   AAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACC                                                                                            >  SRR4302533.2111918/1‑94 (MQ=60)
    AACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCT                                                                                           >  SRR4302533.1323159/1‑94 (MQ=60)
    AACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCT                                                                                           >  SRR4302533.706872/1‑94 (MQ=60)
     ACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTG                                                                                          >  SRR4302533.3473901/1‑94 (MQ=60)
     ACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTG                                                                                          >  SRR4302533.645110/1‑94 (MQ=60)
     ACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTG                                                                                          <  SRR4302533.2989734/94‑1 (MQ=60)
     ACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTG                                                                                          >  SRR4302533.2121430/1‑94 (MQ=60)
     ACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTG                                                                                          >  SRR4302533.2114676/1‑94 (MQ=60)
      CATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGG                                                                                         >  SRR4302533.5599734/1‑94 (MQ=60)
      CATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGG                                                                                         >  SRR4302533.5603807/1‑94 (MQ=60)
        TGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAA                                                                                       >  SRR4302533.2352787/1‑94 (MQ=60)
           ACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAAT                                                                                    <  SRR4302533.4874841/94‑1 (MQ=60)
           ACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAAT                                                                                    <  SRR4302533.960948/94‑1 (MQ=60)
              GGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTC                                                                                 >  SRR4302533.3199639/1‑94 (MQ=60)
                CTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTC                                                                               <  SRR4302533.1967220/94‑1 (MQ=60)
                  GGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCA                                                                             >  SRR4302533.674064/1‑94 (MQ=60)
                   GGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAG                                                                            >  SRR4302533.614372/1‑94 (MQ=60)
                   GGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAG                                                                            >  SRR4302533.4223377/1‑94 (MQ=60)
                   GGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAG                                                                            <  SRR4302533.5649594/94‑1 (MQ=60)
                    GATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGC                                                                           <  SRR4302533.6053529/94‑1 (MQ=60)
                    GATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGC                                                                           <  SRR4302533.404359/94‑1 (MQ=60)
                      TTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGC                                                                         <  SRR4302533.3611492/94‑1 (MQ=60)
                      TTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGC                                                                         >  SRR4302533.5993424/1‑94 (MQ=60)
                      TTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGC                                                                         <  SRR4302533.1839421/94‑1 (MQ=60)
                       TACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCG                                                                        >  SRR4302533.4850032/1‑94 (MQ=60)
                       TACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCG                                                                        <  SRR4302533.2025354/94‑1 (MQ=60)
                       TACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCG                                                                        >  SRR4302533.654666/1‑94 (MQ=60)
                       TACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCG                                                                        >  SRR4302533.3358328/1‑94 (MQ=60)
                        ACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGA                                                                       >  SRR4302533.2629505/1‑94 (MQ=60)
                        ACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGA                                                                       >  SRR4302533.3437899/1‑94 (MQ=60)
                          TCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAAT                                                                     <  SRR4302533.3621333/94‑1 (MQ=60)
                          TCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAAT                                                                     >  SRR4302533.5370725/1‑94 (MQ=60)
                          TCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAAT                                                                     <  SRR4302533.5884273/94‑1 (MQ=60)
                          TCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAAT                                                                     <  SRR4302533.3353030/94‑1 (MQ=60)
                           CTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATC                                                                    >  SRR4302533.5841418/1‑94 (MQ=60)
                           CTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATC                                                                    <  SRR4302533.785400/94‑1 (MQ=60)
                            TTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCG                                                                   >  SRR4302533.3603784/1‑94 (MQ=60)
                               TTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTT                                                                <  SRR4302533.2639907/94‑1 (MQ=60)
                               TTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTT                                                                >  SRR4302533.297350/1‑94 (MQ=60)
                                 CAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTT                                                              >  SRR4302533.5217380/1‑94 (MQ=60)
                                     ACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCA                                                          >  SRR4302533.1653292/1‑94 (MQ=60)
                                      CGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAA                                                         >  SRR4302533.4538488/1‑94 (MQ=60)
                                        TAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGATAAACGGCGAGATGCCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACA                                                       >  SRR4302533.3988620/1‑94 (MQ=60)
                                        TAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACA                                                       >  SRR4302533.264401/1‑94 (MQ=60)
                                        TAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACA                                                       >  SRR4302533.1402410/1‑94 (MQ=60)
                                         AATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAA                                                      >  SRR4302533.2153228/1‑94 (MQ=60)
                                          ATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAA                                                     >  SRR4302533.5898629/1‑94 (MQ=60)
                                              GCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCA                                                 >  SRR4302533.4770709/1‑94 (MQ=60)
                                                CGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACT                                               <  SRR4302533.4124520/94‑1 (MQ=60)
                                                 GCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTA                                              >  SRR4302533.600544/1‑94 (MQ=60)
                                                  CCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTAC                                             <  SRR4302533.334759/94‑1 (MQ=60)
                                                   CAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACG                                            <  SRR4302533.4922100/94‑1 (MQ=60)
                                                   CAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACG                                            <  SRR4302533.3414951/94‑1 (MQ=60)
                                                   CAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACA                                            <  SRR4302533.4529440/94‑1 (MQ=60)
                                                    AGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGC                                           >  SRR4302533.5007936/1‑94 (MQ=60)
                                                      CGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCCCTACCTGGAACATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCA                                         >  SRR4302533.807651/1‑94 (MQ=60)
                                                      CGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCA                                         >  SRR4302533.3636139/1‑94 (MQ=60)
                                                      CGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCA                                         >  SRR4302533.641091/1‑94 (MQ=60)
                                                       GGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAC                                        >  SRR4302533.327315/1‑94 (MQ=60)
                                                       GGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAC                                        >  SRR4302533.539085/1‑94 (MQ=60)
                                                       GGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCAC                                        <  SRR4302533.47067/94‑1 (MQ=60)
                                                        GTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACC                                       >  SRR4302533.5910260/1‑94 (MQ=60)
                                                        GTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACC                                       <  SRR4302533.1324508/94‑1 (MQ=60)
                                                        GTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACC                                       <  SRR4302533.3736041/94‑1 (MQ=60)
                                                         TGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCG                                      <  SRR4302533.4716913/94‑1 (MQ=60)
                                                         TGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCG                                      >  SRR4302533.2870819/1‑94 (MQ=60)
                                                           ACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAC                                    >  SRR4302533.4617311/1‑94 (MQ=60)
                                                           ACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAA                                    <  SRR4302533.147742/94‑1 (MQ=60)
                                                            CGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAAC                                   <  SRR4302533.595471/94‑1 (MQ=60)
                                                            CGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAAC                                   >  SRR4302533.4817044/1‑94 (MQ=60)
                                                            CGGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAAC                                   >  SRR4302533.904922/1‑94 (MQ=60)
                                                             GGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACT                                  <  SRR4302533.1393686/94‑1 (MQ=60)
                                                             GGGCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACT                                  >  SRR4302533.5648476/1‑94 (MQ=60)
                                                               GCAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCT                                <  SRR4302533.2944262/94‑1 (MQ=60)
                                                                CAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTT                               >  SRR4302533.2649907/1‑94 (MQ=60)
                                                                CAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTT                               <  SRR4302533.5715831/94‑1 (MQ=60)
                                                                CAGGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTT                               >  SRR4302533.3018180/1‑94 (MQ=60)
                                                                  GGGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCA                             <  SRR4302533.1323507/94‑1 (MQ=60)
                                                                   GGCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCAT                            <  SRR4302533.5099134/94‑1 (MQ=60)
                                                                    GCAGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATC                           <  SRR4302533.6056831/94‑1 (MQ=60)
                                                                      AGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGC                         <  SRR4302533.788455/94‑1 (MQ=60)
                                                                      AGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGC                         >  SRR4302533.3921296/1‑94 (MQ=60)
                                                                      AGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGC                         <  SRR4302533.4473869/94‑1 (MQ=60)
                                                                      AGAGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGC                         <  SRR4302533.1061274/94‑1 (MQ=60)
                                                                        AGAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTC                       >  SRR4302533.1803056/1‑94 (MQ=60)
                                                                         GAGAAACGGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCT                      >  SRR4302533.971498/1‑94 (MQ=60)
                                                                                GGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACA               >  SRR4302533.4827116/1‑94 (MQ=60)
                                                                                GGCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACA               <  SRR4302533.3606409/94‑1 (MQ=60)
                                                                                 GCGAGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACAT              >  SRR4302533.5884807/1‑94 (MQ=60)
                                                                                    AGATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCA           <  SRR4302533.5512461/94‑1 (MQ=60)
                                                                                    AAATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCA           <  SRR4302533.1176653/94‑1 (MQ=60)
                                                                                      ATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACA         >  SRR4302533.4963409/1‑94 (MQ=60)
                                                                                      ATGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACA         >  SRR4302533.4963408/1‑94 (MQ=60)
                                                                                       TGGCAGTACCTGGAAAATTCCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAA        <  SRR4302533.4662973/94‑1 (MQ=60)
                                                                                       TGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAA        <  SRR4302533.4699738/94‑1 (MQ=60)
                                                                                       TGGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAA        <  SRR4302533.4298756/94‑1 (MQ=60)
                                                                                        GGCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAAC       <  SRR4302533.2712283/94‑1 (MQ=60)
                                                                                         GCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACG      <  SRR4302533.5565/94‑1 (MQ=60)
                                                                                         GCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACG      <  SRR4302533.3326950/94‑1 (MQ=60)
                                                                                         GCACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACG      <  SRR4302533.6001528/94‑1 (MQ=60)
                                                                                           ACTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCA    >  SRR4302533.1592535/1‑94 (MQ=60)
                                                                                            CTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCAT   >  SRR4302533.272609/1‑94 (MQ=60)
                                                                                            CTACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCAT   >  SRR4302533.2469576/1‑94 (MQ=60)
                                                                                             TACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATA  >  SRR4302533.4402722/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAAAAACATGTACGGGCTGGGATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATA  >  NC_000913/1320086‑1320272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑