Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,339,624	A→G	K348R (AAG→AGG)	yciM →	putative heat shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,339,624	0	A	G	99.6%	762.1 / ‑5.4	237	K348R (AAG→AGG)	yciM	putative heat shock protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (126/110); total (126/111)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 255 total aligned reads displayed.

TCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGT  >  NC_000913/1339534‑1339713

tCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAggg                                                                                         >  2:1268603/1‑93 (MQ=255)

tCCATAAGTTAATGGATTACCACTTAAATGAAGCGAAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAggg                                                                                         >  1:4676583/1‑93 (MQ=255)

 ccATAAGTAAAAGGATTTCTAAATAAATGAAGCGGAAGAAGGGCCTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGt                                                                                        <  2:7495474/93‑1 (MQ=255)

   aTAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTag                                                                                      >  2:2897106/1‑92 (MQ=255)

   aTAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTAc                                                                                      >  2:5928262/1‑93 (MQ=255)

    tAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACg                                                                                     <  2:4643214/93‑1 (MQ=255)

    tAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACg                                                                                     >  1:9522307/1‑93 (MQ=255)

     aaGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACgt                                                                                    <  2:7179762/93‑1 (MQ=255)

      aGTTAATGGATTAGCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACgta                                                                                   <  2:9499177/93‑1 (MQ=255)

      aGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACgta                                                                                   >  1:8007477/1‑93 (MQ=255)

      aGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACgta                                                                                   >  1:5672639/1‑93 (MQ=255)

      aGTTAATGGATTACCAATTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACTTGGTTGGCGAGAGAGTACgta                                                                                   <  2:4725084/93‑1 (MQ=255)

       gTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCTTGACATGGTTGGCGAGAGGGTACgtag                                                                                  >  1:8663832/1‑93 (MQ=255)

       gTTAATGGATGACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACgtag                                                                                  <  2:8897858/93‑1 (MQ=255)

        ttAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACgtagt                                                                                 >  2:2078059/1‑93 (MQ=255)

         tAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACgtagta                                                                                >  1:4186664/1‑93 (MQ=255)

         tAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACgtagta                                                                                <  2:5798301/93‑1 (MQ=255)

          aaTGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTaa                                                                               <  2:1051614/93‑1 (MQ=255)

          aaTGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTaa                                                                               >  1:5642019/1‑93 (MQ=255)

           aTGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAg                                                                              <  1:7935396/93‑1 (MQ=255)

            tGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGc                                                                             >  1:3701419/1‑93 (MQ=255)

            tGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGc                                                                             >  2:4568984/1‑93 (MQ=255)

             ggATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGcc                                                                            >  1:2123337/1‑93 (MQ=255)

              gATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCt                                                                           >  1:9115322/1‑93 (MQ=255)

              gATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCt                                                                           >  2:6083611/1‑93 (MQ=255)

               aTTACCACTTAAATGAAGCGGAAGAAGGGGGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGCAAGCCTc                                                                          >  2:3152252/1‑93 (MQ=255)

               aTTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTc                                                                          >  1:2929274/1‑93 (MQ=255)

                 tACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGt                                                                        <  2:8196423/93‑1 (MQ=255)

                   ccACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTa                                                                      <  2:9611899/93‑1 (MQ=255)

                    cACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTAt                                                                     <  1:10015857/93‑1 (MQ=255)

                    cACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTAt                                                                     >  1:2229348/1‑93 (MQ=255)

                     aCTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATc                                                                    >  2:4870524/1‑93 (MQ=255)

                     aCTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATc                                                                    >  2:6238052/1‑93 (MQ=255)

                       ttAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGt                                                                  <  2:7885060/93‑1 (MQ=255)

                       ttAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGt                                                                  >  2:311556/1‑93 (MQ=255)

                        tAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGtt                                                                 >  2:6800373/1‑93 (MQ=255)

                          aaTGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGc                                                               <  2:5632160/93‑1 (MQ=255)

                           aTGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGcc                                                              >  1:1404193/1‑93 (MQ=255)

                           aTGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGcc                                                              >  1:730312/1‑93 (MQ=255)

                            tGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCa                                                             <  1:6586016/93‑1 (MQ=255)

                              aaGCGGAAGAACGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGa                                                           <  1:4021383/93‑1 (MQ=255)

                                gCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTTGCGAGAGGGCACGTAGTAAGCCTCGTTATCGTTGCCAGaaa                                                         >  2:6961042/1‑93 (MQ=255)

                                gCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGaaa                                                         <  1:2537563/93‑1 (MQ=255)

                                gCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGaaa                                                         <  1:3582427/93‑1 (MQ=255)

                                 cGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAAt                                                        <  2:9035263/93‑1 (MQ=255)

                                 cGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAAt                                                        <  2:3973778/93‑1 (MQ=255)

                                    aagaagGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAAtgtg                                                     <  2:4278358/93‑1 (MQ=255)

                                       aagGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGtt                                                  <  2:3377481/93‑1 (MQ=255)

                                        agGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGttt                                                 <  2:5337065/93‑1 (MQ=255)

                                           gCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTAc                                              <  1:9594862/93‑1 (MQ=255)

                                           gCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTACCAGAAATGTGGTTTTAc                                              <  2:4090984/93‑1 (MQ=255)

                                             gTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCg                                            >  1:5213543/1‑93 (MQ=255)

                                               gCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCa                                          <  1:1018243/93‑1 (MQ=255)

                                               gCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCa                                          <  1:506470/93‑1 (MQ=255)

                                                 cAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATa                                        <  2:4846519/93‑1 (MQ=255)

                                                 cAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATa                                        <  1:6914276/93‑1 (MQ=255)

                                                  aaaGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATac                                       >  2:3727615/1‑93 (MQ=255)

                                                  aaaGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATac                                       <  2:5736431/93‑1 (MQ=255)

                                                   aaGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATaca                                      >  1:6261732/1‑93 (MQ=255)

                                                    agagagCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCATAAATGTGGTTTTACCGCATacac                                     >  2:7226448/1‑93 (MQ=255)

                                                     gagagCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACAcc                                    >  1:5332775/1‑93 (MQ=255)

                                                     gagagCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACAcc                                    <  1:8161824/93‑1 (MQ=255)

                                                       gagCCTGGTGGTCCTGGGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCct                                  <  1:7935941/93‑1 (MQ=255)

                                                       gagCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACAACct                                  >  2:2796501/1‑93 (MQ=255)

                                                        agCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCctc                                 <  1:5466179/93‑1 (MQ=255)

                                                         gCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCctct                                <  2:5218636/93‑1 (MQ=255)

                                                          ccTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTa                               <  1:4506784/93‑1 (MQ=255)

                                                          ccTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTa                               >  2:8814373/1‑93 (MQ=255)

                                                          ccTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTa                               >  1:3170867/1‑93 (MQ=255)

                                                           cTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTAc                              <  1:7722542/93‑1 (MQ=255)

                                                            tGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACt                             <  2:8361904/93‑1 (MQ=255)

                                                              aTGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTgg                           >  2:8079067/1‑93 (MQ=255)

                                                              aTGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTgg                           >  2:7836367/1‑93 (MQ=255)

                                                              aTGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTgg                           >  1:9955202/1‑93 (MQ=255)

                                                              aTGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTgg                           >  2:1522202/1‑93 (MQ=255)

                                                              aTGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTgc                           >  1:287444/1‑92 (MQ=255)

                                                                ggTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCa                         >  2:7333676/1‑93 (MQ=255)

                                                                 gTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCAt                        >  1:2189008/1‑93 (MQ=255)

                                                                    ctgcGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGt                     <  1:73771/93‑1 (MQ=255)

                                                                     tgcGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTc                    >  1:937366/1‑93 (MQ=255)

                                                                      gcGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTCCGGCATACCCCCTCTACTGCCATTGTcc                   >  1:8267552/1‑93 (MQ=255)

                                                                      gcGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTcc                   <  2:5708517/93‑1 (MQ=255)

                                                                      gcGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTcc                   >  1:7946799/1‑93 (MQ=255)

                                                                        gTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGt                 >  1:8374950/1‑93 (MQ=255)

                                                                         tGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTc                >  1:6029812/1‑93 (MQ=255)

                                                                         tGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTc                >  2:3682270/1‑93 (MQ=255)

                                                                           aCATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCtt              >  1:7434178/1‑93 (MQ=255)

                                                                            cATGGTTGGCGAGAGGGTACGTCGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTg             <  1:8923973/93‑1 (MQ=255)

                                                                            cATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTg             <  1:6119979/93‑1 (MQ=255)

                                                                             aTGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGt            <  2:8781995/93‑1 (MQ=255)

                                                                              tGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTc           >  1:236469/1‑93 (MQ=255)

                                                                              tGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTc           <  2:9757159/93‑1 (MQ=255)

                                                                              tGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTc           >  2:7590200/1‑93 (MQ=255)

                                                                              tGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTc           <  2:431015/93‑1 (MQ=255)

                                                                               ggTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCg          <  1:7016657/93‑1 (MQ=255)

                                                                               ggTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCg          <  1:8458931/93‑1 (MQ=255)

                                                                               ggTGGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATGCACCCTCTACTGGCATTGTCCGTCTTGTCg          <  1:5887871/93‑1 (MQ=255)

                                                                                 ttGGCGAGAGGTTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCggg        >  1:9738971/1‑93 (MQ=255)

                                                                                   ggCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGcc      >  1:4913958/1‑93 (MQ=255)

                                                                                       agagGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGt  >  2:7129608/1‑93 (MQ=255)

TCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGT  >  NC_000913/1339534‑1339713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCA  >  NC_000913/1339531‑1339715

TGTTCCATAAGTTAATTGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAG                                                                                             <  SRR4302540.5823582/94‑1 (MQ=60)

TGTTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAG                                                                                             <  SRR4302540.5823581/94‑1 (MQ=60)

 GTTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGG                                                                                            >  SRR4302540.3633183/1‑94 (MQ=60)

  TTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGCCATGGTTGGCGCGAGGG                                                                                           >  SRR4302540.566646/1‑94 (MQ=60)

  TTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGG                                                                                           >  SRR4302540.566640/1‑94 (MQ=60)

  TTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGG                                                                                           <  SRR4302540.9280284/94‑1 (MQ=60)

  TTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGG                                                                                           >  SRR4302540.4726622/1‑94 (MQ=60)

  TTCCATAAGTTAATGGATTACCACTTAAATGAAGCGAAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGG                                                                                           >  SRR4302540.4726621/1‑94 (MQ=60)

   TCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGT                                                                                          >  SRR4302540.700389/1‑94 (MQ=60)

    CCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTA                                                                                         >  SRR4302540.9673624/1‑94 (MQ=60)

     CATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTAC                                                                                        <  SRR4302540.6205998/94‑1 (MQ=60)

     CATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTAC                                                                                        <  SRR4302540.747959/94‑1 (MQ=60)

      ATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACG                                                                                       >  SRR4302540.3527465/1‑94 (MQ=60)

      ATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACG                                                                                       >  SRR4302540.6785659/1‑94 (MQ=60)

      ATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACG                                                                                       <  SRR4302540.4258775/94‑1 (MQ=60)

      ATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACG                                                                                       >  SRR4302540.9635063/1‑94 (MQ=60)

        AAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTA                                                                                     >  SRR4302540.8100652/1‑94 (MQ=60)

        AAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTA                                                                                     >  SRR4302540.5735101/1‑94 (MQ=60)

         AGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCTTGACATGGTTGGCGAGAGGGTACGTAG                                                                                    >  SRR4302540.8764459/1‑94 (MQ=60)

         AGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAG                                                                                    <  SRR4302540.7718149/94‑1 (MQ=60)

           TTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTA                                                                                  >  SRR4302540.4232174/1‑94 (MQ=60)

           TTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTA                                                                                  <  SRR4302540.1332693/94‑1 (MQ=60)

           TTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTA                                                                                  <  SRR4302540.7458935/94‑1 (MQ=60)

            tatgGGATTACCACTTAAATGAAGC‑GAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAG                                                                                <  SRR4302540.7947646/90‑1 (MQ=60)

            TAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAA                                                                                 <  SRR4302540.2323018/94‑1 (MQ=60)

            TAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAA                                                                                 >  SRR4302540.5704114/1‑94 (MQ=60)

              ATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGC                                                                               <  SRR4302540.8027886/94‑1 (MQ=60)

              ATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGC                                                                               >  SRR4302540.3741827/1‑94 (MQ=60)

               TGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCC                                                                              >  SRR4302540.2146112/1‑94 (MQ=60)

                GGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCT                                                                             >  SRR4302540.9224886/1‑94 (MQ=60)

                 GATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTC                                                                            >  SRR4302540.2705652/1‑94 (MQ=60)

                 GATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTC                                                                            >  SRR4302540.2961397/1‑94 (MQ=60)

                 GATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTC                                                                            >  SRR4302540.6127943/1‑94 (MQ=60)

                 GATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTC                                                                            <  SRR4302540.8031234/94‑1 (MQ=60)

                 GATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTC                                                                            <  SRR4302540.4816638/94‑1 (MQ=60)

                  ATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCG                                                                           >  SRR4302540.8972335/1‑94 (MQ=60)

                    TACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTT                                                                         <  SRR4302540.8034241/94‑1 (MQ=60)

                      CCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTAT                                                                       >  SRR4302540.2253356/1‑94 (MQ=60)

                       CACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATC                                                                      <  SRR4302540.10132446/94‑1 (MQ=60)

                       CACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATC                                                                      <  SRR4302540.10132447/94‑1 (MQ=60)

                       CACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATC                                                                      <  SRR4302540.1009770/94‑1 (MQ=60)

                       CACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATC                                                                      >  SRR4302540.7028164/1‑94 (MQ=60)

                        ACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCG                                                                     >  SRR4302540.7475339/1‑94 (MQ=60)

                         CTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGT                                                                    >  SRR4302540.6372965/1‑94 (MQ=60)

                          TTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTT                                                                   <  SRR4302540.5008841/94‑1 (MQ=60)

                            AAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTAGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGC                                                                 >  SRR4302540.1698098/1‑94 (MQ=60)

                             AATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCC                                                                >  SRR4302540.1419313/1‑94 (MQ=60)

                             AATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCC                                                                >  SRR4302540.738131/1‑94 (MQ=60)

                             AATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCC                                                                <  SRR4302540.5785173/94‑1 (MQ=60)

                               TGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAG                                                              <  SRR4302540.6658968/94‑1 (MQ=60)

                                 AAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAA                                                            <  SRR4302540.4064718/94‑1 (MQ=60)

                                 AAGCGGAAGAACGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAA                                                            <  SRR4302540.4064716/94‑1 (MQ=60)

                                  AGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAA                                                           >  SRR4302540.2442583/1‑94 (MQ=60)

                                   GCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAAT                                                          <  SRR4302540.2564929/94‑1 (MQ=60)

                                   GCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAAT                                                          <  SRR4302540.3621552/94‑1 (MQ=60)

                                    CGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATG                                                         >  SRR4302540.2187338/1‑94 (MQ=60)

                                     GGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGT                                                        >  SRR4302540.4352474/1‑94 (MQ=60)

                                     GGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGT                                                        >  SRR4302540.5211888/1‑94 (MQ=60)

                                     GGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGG                                                        >  SRR4302540.3963680/1‑94 (MQ=60)

                                       AAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGG                                                      <  SRR4302540.9971557/94‑1 (MQ=60)

                                       AAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGG                                                      >  SRR4302540.8532759/1‑94 (MQ=60)

                                        AGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGT                                                     >  SRR4302540.5418274/1‑94 (MQ=60)

                                            GGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTA                                                 <  SRR4302540.7245753/94‑1 (MQ=60)

                                            GGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTA                                                 >  SRR4302540.8214936/1‑94 (MQ=60)

                                             GGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTAC                                                >  SRR4302540.8318590/1‑94 (MQ=60)

                                              GCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACC                                               <  SRR4302540.9708086/94‑1 (MQ=60)

                                               CGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCG                                              >  SRR4302540.5270356/1‑94 (MQ=60)

                                                GTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGC                                             >  SRR4302540.9573569/1‑94 (MQ=60)

                                                GTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAAGGTGGTTTTACaga                                             >  SRR4302540.6433627/1‑91 (MQ=60)

                                                GTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGGACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACaga                                             >  SRR4302540.8622806/1‑91 (MQ=60)

                                                 TGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCA                                            >  SRR4302540.5302151/1‑94 (MQ=60)

                                                  GCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCAT                                           <  SRR4302540.1028573/94‑1 (MQ=60)

                                                  GCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCAT                                           <  SRR4302540.511894/94‑1 (MQ=60)

                                                    CAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATAC                                         <  SRR4302540.8026721/94‑1 (MQ=60)

                                                    CAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATAC                                         <  SRR4302540.6991216/94‑1 (MQ=60)

                                                     AAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACA                                        >  SRR4302540.7097221/1‑94 (MQ=60)

                                                     AAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACA                                        >  SRR4302540.6331395/1‑94 (MQ=60)

                                                     AAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTAATCGTTGCCAGAAATGTGGTTTTACCGCATACA                                        >  SRR4302540.9808017/1‑94 (MQ=60)

                                                      AAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACAC                                       <  SRR4302540.8106794/94‑1 (MQ=60)

                                                       AGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACC                                      >  SRR4302540.5391212/1‑94 (MQ=60)

                                                        GAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCC                                     <  SRR4302540.5194505/94‑1 (MQ=60)

                                                        GAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCC                                     <  SRR4302540.8256597/94‑1 (MQ=60)

                                                         AGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCT                                    >  SRR4302540.9457637/1‑94 (MQ=60)

                                                          GAGCCTGGTGGTCCTGGGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTC                                   <  SRR4302540.8028435/94‑1 (MQ=60)

                                                          GAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTC                                   <  SRR4302540.8028434/94‑1 (MQ=60)

                                                           AGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCT                                  <  SRR4302540.5526349/94‑1 (MQ=60)

                                                           AGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCGCT                                  >  SRR4302540.5727921/1‑94 (MQ=60)

                                                            GCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTA                                 <  SRR4302540.9456404/94‑1 (MQ=60)

                                                            GCCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTA                                 >  SRR4302540.3205336/1‑94 (MQ=60)

                                                             CCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTAC                                >  SRR4302540.9937623/1‑94 (MQ=60)

                                                             CCTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTAC                                <  SRR4302540.4555629/94‑1 (MQ=60)

                                                             CCTCATGCTGCTGCGTGACATGGTTGCCGAGAGGCTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTAC                                <  SRR4302540.2291781/94‑1 (MQ=60)

                                                              CTGATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACT                               <  SRR4302540.7812319/94‑1 (MQ=60)

                                                                GATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGG                             >  SRR4302540.10071402/1‑94 (MQ=60)

                                                                GATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGG                             >  SRR4302540.290542/1‑94 (MQ=60)

                                                                GATGGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGC                             >  SRR4302540.290283/1‑94 (MQ=60)

                                                                   GGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCAT                          >  SRR4302540.2212641/1‑94 (MQ=60)

                                                                   GGTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCAT                          >  SRR4302540.2212642/1‑94 (MQ=60)

                                                                    GTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATT                         <  SRR4302540.3841619/94‑1 (MQ=60)

                                                                    GTGCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATT                         >  SRR4302540.3601904/1‑94 (MQ=60)

                                                                      GCTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGT                       >  SRR4302540.4579105/1‑94 (MQ=60)

                                                                       CTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTC                      >  SRR4302540.60201/1‑94 (MQ=60)

                                                                       CTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTC                      >  SRR4302540.947183/1‑94 (MQ=60)

                                                                       CTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTC                      <  SRR4302540.9651991/94‑1 (MQ=60)

                                                                       CTGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTC                      <  SRR4302540.74538/94‑1 (MQ=60)

                                                                       CTGCGTGACATGGTTGGCCAGAGGGTACGTATTAAGCCTCGTTATCGTTTCCAGAAATGTGGTTTTACCGCATACCCCCTCTACTGGCATTGTC                      <  SRR4302540.3059492/94‑1 (MQ=60)

                                                                        TGCGTGACATGGTTGGCGAGAGGGTCCGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTATCGCATTCACCCTCTACTGGCCTTGTCC                     >  SRR4302540.9027834/1‑94 (MQ=60)

                                                                        TGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTCCGGCATACCCCCTCTACTGCCATTGTCC                     >  SRR4302540.8363613/1‑94 (MQ=60)

                                                                        TGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCC                     >  SRR4302540.8363614/1‑94 (MQ=60)

                                                                        TGCGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCC                     >  SRR4302540.8039433/1‑94 (MQ=60)

                                                                          CGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGT                   <  SRR4302540.3673561/94‑1 (MQ=60)

                                                                          CGTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGT                   >  SRR4302540.8472134/1‑94 (MQ=60)

                                                                           GTGACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTC                  >  SRR4302540.6096726/1‑94 (MQ=60)

                                                                             GACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTT                <  SRR4302540.5384128/94‑1 (MQ=60)

                                                                             GACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTT                >  SRR4302540.7517002/1‑94 (MQ=60)

                                                                             GACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTT                >  SRR4302540.2714919/1‑94 (MQ=60)

                                                                             GACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTT                <  SRR4302540.1406946/94‑1 (MQ=60)

                                                                             GACATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTT                >  SRR4302540.6153132/1‑94 (MQ=60)

                                                                               CATGGTTGGCGAGAGGGTACGTCGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGT              <  SRR4302540.9027614/94‑1 (MQ=60)

                                                                               CATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGT              <  SRR4302540.6188138/94‑1 (MQ=60)

                                                                                ATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTC             >  SRR4302540.238762/1‑94 (MQ=60)

                                                                                ATGGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTC             <  SRR4302540.2234816/94‑1 (MQ=60)

                                                                                 TGG‑TGGCGAGAGGGTACGGAGTAAGCCTGGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGG           <  SRR4302540.8557144/94‑1 (MQ=60)

                                                                                  GGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGG           <  SRR4302540.8914455/94‑1 (MQ=60)

                                                                                  GGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGG           <  SRR4302540.8557145/94‑1 (MQ=60)

                                                                                  GGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGG           <  SRR4302540.7094571/94‑1 (MQ=60)

                                                                                  GGTTGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGG           <  SRR4302540.5822253/94‑1 (MQ=60)

                                                                                  GGTGGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATGCACCCTCTACTGGCATTGTCCGTCTTGTCGG           <  SRR4302540.5952922/94‑1 (MQ=60)

                                                                                   GTTGGCGAGAGGTTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGG          >  SRR4302540.9853305/1‑94 (MQ=60)

                                                                                     TGGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCC        >  SRR4302540.4966870/1‑94 (MQ=60)

                                                                                     TCGCGAGAGGGTACGTAGTAAGCCTCGTTATCCTTCCCAGAAATGTGGTTTTACCGCATACCCCCTCTACTGGCATTGTCCGTCTTGTCGGGCC        <  SRR4302540.1978394/94‑1 (MQ=60)

                                                                                      GGCGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCT       <  SRR4302540.8055058/94‑1 (MQ=60)

                                                                                        CGAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGG     <  SRR4302540.8317823/94‑1 (MQ=60)

                                                                                         GAGAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGT    >  SRR4302540.3051448/1‑94 (MQ=60)

                                                                                           GAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCA  >  SRR4302540.1326928/1‑94 (MQ=60)

                                                                                           GAGGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCA  <  SRR4302540.6271920/94‑1 (MQ=60)

TGTTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCA  >  NC_000913/1339531‑1339715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`