Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,372,616	T→C	I168T (ATC→ACC)	ycjP →	putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,372,616	0	T	C	98.2%	686.0 / 0.9	221	I168T (ATC→ACC)	ycjP	putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (100/117); total (102/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

Only 100 of 237 total aligned reads displayed.

TTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCATCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCG  >  NC_000913/1372526‑1372705

tttACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCAccc                                                                                         >  1:2606996/1‑93 (MQ=255)

   aCGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAGAAGCTACTTCGACACCACCCCCg                                                                                      >  2:4490500/1‑93 (MQ=255)

   aCGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCg                                                                                      >  2:4415028/1‑93 (MQ=255)

   aCGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCg                                                                                      >  2:6157991/1‑93 (MQ=255)

    cGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGa                                                                                     <  1:2175339/93‑1 (MQ=255)

    cGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGa                                                                                     <  1:7776019/93‑1 (MQ=255)

     gACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGAt                                                                                    >  1:8743383/1‑93 (MQ=255)

     gACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGAt                                                                                    <  1:2725625/93‑1 (MQ=255)

      acacTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATg                                                                                   <  1:1566399/93‑1 (MQ=255)

       cacTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATga                                                                                  <  2:8768214/93‑1 (MQ=255)

       cacTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATga                                                                                  <  1:8005271/93‑1 (MQ=255)

        acTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATgag                                                                                 <  2:8556830/93‑1 (MQ=255)

         cTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATgaga                                                                                >  2:3044253/1‑93 (MQ=255)

         cTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATgaga                                                                                <  2:131018/93‑1 (MQ=255)

             gaTGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCga                                                                            <  1:7494198/93‑1 (MQ=255)

             gaTGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCga                                                                            <  1:996594/93‑1 (MQ=255)

              aTGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCgaa                                                                           <  1:9926712/93‑1 (MQ=255)

               tGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCgaag                                                                          <  1:7044754/93‑1 (MQ=255)

               tGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCgaag                                                                          >  1:4314419/1‑93 (MQ=255)

                ggCGCTGATCATCACCATGGTGACGCAAGCTCTGCCCCCCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCgaaga                                                                         <  2:7298616/93‑1 (MQ=255)

                ggCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCgaaga                                                                         >  2:4149205/1‑93 (MQ=255)

                 gcgcTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCgaagaa                                                                        <  1:3897153/93‑1 (MQ=255)

                 gcgcTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCgaagaa                                                                        <  2:8215463/93‑1 (MQ=255)

                     tGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAgcgg                                                                    <  1:494499/93‑1 (MQ=255)

                      gATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAgcggc                                                                   >  2:9851906/1‑93 (MQ=255)

                       atcatcACCATGGTGACGCAAACTCTGCCCAGCGCCGTGTTTATGCTGGAAAGCTACTTCGAGACCACCCCAGATGAGATCGAAGAAgcggcg                                                                  >  1:5301171/1‑93 (MQ=255)

                        tcatcaCCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCga                                                                 >  2:6872926/1‑93 (MQ=255)

                        tcatcaCCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCga                                                                 >  2:8189683/1‑93 (MQ=255)

                         catcaCCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCgat                                                                <  2:9326399/93‑1 (MQ=255)

                          atcaCCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCgatg                                                               >  1:6611512/1‑93 (MQ=255)

                           tcaCCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCgatga                                                              <  1:7931308/93‑1 (MQ=255)

                           tcaCCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCgatga                                                              <  2:2423873/93‑1 (MQ=255)

                            caCCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCATCCCCGATGAGATCGAAGAAGCGGCgatgat                                                             <  1:9393721/93‑1 (MQ=255)

                             aCCATGGTGACGCAAACTCTGCCCATCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGatgatg                                                            <  2:5229527/93‑1 (MQ=255)

                              ccATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATgatgg                                                           >  1:9630304/1‑93 (MQ=255)

                              ccATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATgatgg                                                           >  1:4543612/1‑93 (MQ=255)

                               cATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATgatgga                                                          <  2:194912/93‑1 (MQ=255)

                               cATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATgatgga                                                          <  1:6720424/93‑1 (MQ=255)

                                aTGGGGACTCAAGCTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTCCTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATgatggat                                                         <  2:9062016/93‑1 (MQ=255)

                                 tGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATgatggatg                                                        <  2:9127944/93‑1 (MQ=255)

                                 tGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATgatggatg                                                        >  2:1729229/1‑93 (MQ=255)

                                    tGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGcc                                                     <  2:3955115/93‑1 (MQ=255)

                                     gACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCt                                                    <  2:863284/93‑1 (MQ=255)

                                      aCGCAAACTCTGCCCGCCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTc                                                   <  1:7321675/93‑1 (MQ=255)

                                      aCGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTc                                                   >  2:3764398/1‑93 (MQ=255)

                                        gCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCATCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCaa                                                 >  1:3917259/1‑93 (MQ=255)

                                         cAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAAc                                                <  1:9133117/93‑1 (MQ=255)

                                         cAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAAc                                                <  2:1886423/93‑1 (MQ=255)

                                          aaaCTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAAcc                                               <  2:2471135/93‑1 (MQ=255)

                                            aCTGTGCCTCCCGCCGTGTTTATGCTGAAAAGCTCCTTCGGCACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCACCCGt                                             <  1:2928160/93‑1 (MQ=255)

                                             ctctGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTc                                            <  2:6118816/93‑1 (MQ=255)

                                              tctGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCt                                           <  2:5685390/93‑1 (MQ=255)

                                              tctGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCt                                           >  2:7557137/1‑93 (MQ=255)

                                              ccagCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCt                                           <  2:8598049/90‑1 (MQ=255)

                                               ctGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTg                                          >  1:2848616/1‑93 (MQ=255)

                                               ctGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTg                                          >  2:6597171/1‑93 (MQ=255)

                                                    cACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAAt                                     >  1:3327753/1‑93 (MQ=255)

                                                    cACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAAt                                     <  2:3521428/93‑1 (MQ=255)

                                                      ccgccgTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTa                                   <  1:4896359/93‑1 (MQ=255)

                                                      ccgccgTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTa                                   >  1:3978070/1‑93 (MQ=255)

                                                        gccgTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATc                                 <  2:8115706/93‑1 (MQ=255)

                                                        gacgTGTTTATGCTGAACAGCTCCTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATc                                 <  2:4439161/91‑1 (MQ=255)

                                                         ccgTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCt                                <  2:2660696/93‑1 (MQ=255)

                                                         ccgTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCt                                <  2:9707669/93‑1 (MQ=255)

                                                         ccgTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCt                                >  1:5441954/1‑93 (MQ=255)

                                                         ccgTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCt                                <  2:671310/93‑1 (MQ=255)

                                                         ccgTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCt                                <  1:9205868/93‑1 (MQ=255)

                                                          cgTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCtt                               >  2:1217579/1‑93 (MQ=255)

                                                           gtgtTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTc                              >  2:888551/1‑93 (MQ=255)

                                                             gtTTATGCTTAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCg                            <  1:8555740/93‑1 (MQ=255)

                                                             gtTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCg                            >  2:4053279/1‑93 (MQ=255)

                                                             gtTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCg                            <  1:9142487/93‑1 (MQ=255)

                                                              tttATGCTGAAAAGCGACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGc                           <  2:6314849/93‑1 (MQ=255)

                                                                tATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCAt                         <  2:9389191/93‑1 (MQ=255)

                                                                   gCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCAc                      >  2:1430664/1‑93 (MQ=255)

                                                                   gCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCAc                      <  2:9286907/93‑1 (MQ=255)

                                                                    cTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCAcc                     >  1:8353843/1‑93 (MQ=255)

                                                                     tGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCg                    >  1:5947536/1‑93 (MQ=255)

                                                                      gAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGt                   <  2:7866307/93‑1 (MQ=255)

                                                                        aaaGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGc                 <  1:4084293/93‑1 (MQ=255)

                                                                        aaaGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGc                 >  1:5673583/1‑93 (MQ=255)

                                                                        aaaGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGc                 >  1:2304670/1‑93 (MQ=255)

                                                                        aaaGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGc                 >  2:3423865/1‑93 (MQ=255)

                                                                         aaGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGcc                <  2:3351188/93‑1 (MQ=255)

                                                                         aaGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGcc                <  1:1958269/93‑1 (MQ=255)

                                                                         aaGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGcc                >  1:1236245/1‑93 (MQ=255)

                                                                         aaGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGcc                >  2:7068808/1‑93 (MQ=255)

                                                                         aaGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGcc                <  2:1964531/93‑1 (MQ=255)

                                                                          aGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCg               >  1:1669665/1‑93 (MQ=255)

                                                                          aGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCg               >  2:1242401/1‑93 (MQ=255)

                                                                            cTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGtt             <  2:2977460/93‑1 (MQ=255)

                                                                             tACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTg            <  1:3086186/93‑1 (MQ=255)

                                                                              aCTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTgg           >  2:4225137/1‑93 (MQ=255)

                                                                               catCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGc          <  1:2438896/91‑1 (MQ=255)

                                                                                ttCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCg         >  1:8672449/1‑93 (MQ=255)

                                                                                 tCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGa        >  2:6218400/1‑93 (MQ=255)

                                                                                  cGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCCCCGTGCCGTTGGCGAt       <  2:1508659/93‑1 (MQ=255)

                                                                                     caccacCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTc    >  1:8398190/1‑93 (MQ=255)

                                                                                       ccACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCg  >  1:8090941/1‑93 (MQ=255)

                                                                                       ccACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCg  >  2:6538987/1‑93 (MQ=255)

TTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCATCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCG  >  NC_000913/1372526‑1372705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCATTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCATCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGGGCT  >  NC_000913/1372523‑1372709

GCATTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAACGCTACTTCGACACCAC                                                                                               >  SRR4302540.5109286/1‑94 (MQ=60)

GCATTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCAC                                                                                               >  SRR4302540.9500932/1‑94 (MQ=60)

GCATTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCAC                                                                                               >  SRR4302540.5109287/1‑94 (MQ=60)

 CATTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACC                                                                                              <  SRR4302540.6771918/94‑1 (MQ=60)

 CATTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACC                                                                                              >  SRR4302540.1916120/1‑94 (MQ=60)

 CATTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTCTTTATGCTGAAAAGCTACTTCGACACCACC                                                                                              >  SRR4302540.5112482/1‑94 (MQ=60)

  ATTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCC                                                                                             >  SRR4302540.2635130/1‑94 (MQ=60)

  ATTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCC                                                                                             <  SRR4302540.8028974/94‑1 (MQ=60)

    TTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCC                                                                                           <  SRR4302540.2757047/94‑1 (MQ=60)

     TACGACACTGAGATGGCGCTGATCCTCCCCATGGTGACGCAAACTCTCCCCACCGCAGTGCTTCTGCTGAAAAGCTACTTCCGCACCACCCCCG                                                                                          >  SRR4302540.9115424/1‑94 (MQ=57)

     TACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCG                                                                                          >  SRR4302540.9115423/1‑94 (MQ=60)

       CGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGAT                                                                                        <  SRR4302540.7866924/94‑1 (MQ=60)

       CGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGAT                                                                                        >  SRR4302540.8844867/1‑94 (MQ=60)

       CGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGAT                                                                                        <  SRR4302540.8195042/94‑1 (MQ=60)

       CGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGAT                                                                                        <  SRR4302540.2198797/94‑1 (MQ=60)

        GACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATG                                                                                       <  SRR4302540.2755020/94‑1 (MQ=60)

         ACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGA                                                                                      <  SRR4302540.1583584/94‑1 (MQ=60)

          CACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAG                                                                                     <  SRR4302540.8098429/94‑1 (MQ=60)

           ACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGA                                                                                    >  SRR4302540.4205395/1‑94 (MQ=60)

            CTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGAT                                                                                   >  SRR4302540.7380055/1‑94 (MQ=60)

                GATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAA                                                                               <  SRR4302540.7577897/94‑1 (MQ=60)

                GATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAA                                                                               <  SRR4302540.1006736/94‑1 (MQ=60)

                 ATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAG                                                                              <  SRR4302540.10042602/94‑1 (MQ=60)

                 ATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAG                                                                              >  SRR4302540.4361391/1‑94 (MQ=60)

                 ATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAG                                                                              <  SRR4302540.10042603/94‑1 (MQ=60)

                 ATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAG                                                                              >  SRR4302540.9374117/1‑94 (MQ=60)

                  TGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGA                                                                             <  SRR4302540.7123090/94‑1 (MQ=60)

                   GGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAA                                                                            <  SRR4302540.2399712/94‑1 (MQ=60)

                   GGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAA                                                                            <  SRR4302540.7588576/94‑1 (MQ=60)

                    GCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAG                                                                           <  SRR4302540.5471957/94‑1 (MQ=60)

                    GCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAG                                                                           <  SRR4302540.486832/94‑1 (MQ=60)

                    GCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAG                                                                           <  SRR4302540.3939445/94‑1 (MQ=60)

                     CGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACTCCCGATGAGATCGAAGAAGC                                                                          >  SRR4302540.8237299/1‑94 (MQ=60)

                      GCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCG                                                                         <  SRR4302540.8554411/94‑1 (MQ=60)

                       CTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGG                                                                        >  SRR4302540.1397563/1‑94 (MQ=60)

                        TGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGC                                                                       <  SRR4302540.499800/94‑1 (MQ=60)

                        TGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGC                                                                       <  SRR4302540.3960860/94‑1 (MQ=60)

                         GATCATCACCATGGTGACGCAAACTCTGCCCAGCGCCGTGTTTATGCTGGAAAGCTACTTCGAGACCACCCCAGATGAGATCGAAGAAGCGGCG                                                                      >  SRR4302540.5359174/1‑94 (MQ=60)

                         GATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCG                                                                      >  SRR4302540.2569818/1‑94 (MQ=60)

                          ATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGA                                                                     >  SRR4302540.5282703/1‑94 (MQ=60)

                           TCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGCAGAAGCGGCGAT                                                                    >  SRR4302540.8777126/1‑94 (MQ=60)

                           TCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGAT                                                                    >  SRR4302540.2303920/1‑94 (MQ=60)

                           TCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGAT                                                                    >  SRR4302540.8777127/1‑94 (MQ=60)

                            CATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATG                                                                   >  SRR4302540.6684721/1‑94 (MQ=60)

                             ATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGA                                                                  >  SRR4302540.5378013/1‑94 (MQ=60)

                              TCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGAT                                                                 <  SRR4302540.8023754/94‑1 (MQ=60)

                              TCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGAT                                                                 >  SRR4302540.439190/1‑94 (MQ=60)

                               CACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCATCCCCGATGAGATCGAAGAAGCGGCGATGATG                                                                <  SRR4302540.9505503/94‑1 (MQ=60)

                                ACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGG                                                               >  SRR4302540.9743784/1‑94 (MQ=60)

                                ACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGG                                                               >  SRR4302540.4592778/1‑94 (MQ=60)

                                 CCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGA                                                              >  SRR4302540.2781346/1‑94 (MQ=60)

                                  CATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCTAAGAAGCGGCGATGATGGAT                                                             >  SRR4302540.470488/1‑94 (MQ=60)

                                  CATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGAT                                                             <  SRR4302540.6794590/94‑1 (MQ=60)

                                  CATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGAT                                                             <  SRR4302540.928655/94‑1 (MQ=60)

                                  CATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGAT                                                             <  SRR4302540.3170584/94‑1 (MQ=60)

                                  CATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGAT                                                             <  SRR4302540.4229281/94‑1 (MQ=60)

                                  CATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGAT                                                             <  SRR4302540.10074104/94‑1 (MQ=60)

                                    TGGTGACGCAAACTCTTCCCACCGCCGTGTTTATGCTTAAAAGCTACTTAGACACCACCCCCGATGAGATCGAAGAATCGGCCATGATGcatgt                                                           >  SRR4302540.1995930/1‑89 (MQ=60)

                                    TGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGGTGGATGG                                                           <  SRR4302540.6342990/94‑1 (MQ=60)

                                    TGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGG                                                           <  SRR4302540.8155900/94‑1 (MQ=60)

                                         ACGCAAACTCTGCCCGCCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCA                                                      <  SRR4302540.7403449/94‑1 (MQ=60)

                                         ACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCA                                                      <  SRR4302540.8039478/94‑1 (MQ=60)

                                          CGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCATCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAA                                                     >  SRR4302540.3959670/1‑94 (MQ=60)

                                           GCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAAC                                                    <  SRR4302540.8715227/94‑1 (MQ=60)

                                           GCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAAC                                                    >  SRR4302540.1886070/1‑94 (MQ=60)

                                            CAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACC                                                   <  SRR4302540.40528/94‑1 (MQ=60)

                                            CAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACC                                                   <  SRR4302540.9242883/94‑1 (MQ=60)

                                               actgtgcctcCCGCCGTGTTTATGCTGAAAAGCTCCTTCGGCACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCACCCGTC                                                <  SRR4302540.2960267/84‑1 (MQ=60)

                                               ACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGCCGATGATGGATGGCCTCAACCGTC                                                >  SRR4302540.7731958/1‑94 (MQ=60)

                                               ACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCACCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTC                                                <  SRR4302540.2960268/94‑1 (MQ=60)

                                                CTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCT                                               >  SRR4302540.7956159/1‑94 (MQ=60)

                                                CTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCT                                               >  SRR4302540.9601791/1‑94 (MQ=60)

                                                 TCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTG                                              >  SRR4302540.2879465/1‑94 (MQ=60)

                                                    GCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAA                                           <  SRR4302540.3610125/94‑1 (MQ=60)

                                                      CCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAAT                                         >  SRR4302540.7567287/1‑94 (MQ=60)

                                                      CCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAAT                                         >  SRR4302540.3364275/1‑94 (MQ=60)

                                                      CCAACGCCGTGTTTATGCTGAAAAGCTACTTCGACAACACCCCCGATGAGATCGAAGAAGCGGGGATGAgggagggccacacctgtctgcaatt                                         >  SRR4302540.3364274/1‑69 (MQ=60)

                                                        ACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTA                                       >  SRR4302540.4020924/1‑94 (MQ=60)

                                                         CCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTAT                                      <  SRR4302540.4949022/94‑1 (MQ=60)

                                                           GCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCT                                    >  SRR4302540.5501833/1‑94 (MQ=60)

                                                           GCCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCT                                    >  SRR4302540.3077625/1‑94 (MQ=60)

                                                            CCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTT                                   <  SRR4302540.9316329/94‑1 (MQ=60)

                                                            CCGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTT                                   >  SRR4302540.5721916/1‑94 (MQ=60)

                                                            CCGTGTTTATGCTGAAAAGCTACTGCCACCCCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTT                                   <  SRR4302540.9316328/94‑1 (MQ=60)

                                                             CGTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTC                                  >  SRR4302540.6059178/1‑94 (MQ=60)

                                                              GTGTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCC                                 >  SRR4302540.9898731/1‑94 (MQ=60)

                                                                GTTTATGCTTAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGC                               <  SRR4302540.8655116/94‑1 (MQ=60)

                                                                GTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGC                               <  SRR4302540.9252316/94‑1 (MQ=60)

                                                                GTTTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGC                               >  SRR4302540.6756887/1‑94 (MQ=60)

                                                                  TTATGCTGAAAAGCTACTTCGACACCATCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCAT                             >  SRR4302540.2953724/1‑94 (MQ=60)

                                                                  TTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCAT                             <  SRR4302540.5989604/94‑1 (MQ=60)

                                                                  TTATGCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCAT                             <  SRR4302540.8110898/94‑1 (MQ=60)

                                                                      GCTGAAAAGCTACTTCGACACCCCCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACC                         <  SRR4302540.1976468/94‑1 (MQ=60)

                                                                      GCTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACC                         >  SRR4302540.8450803/1‑94 (MQ=60)

                                                                       CTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCG                        >  SRR4302540.2741561/1‑94 (MQ=60)

                                                                       CTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCG                        >  SRR4302540.6013294/1‑94 (MQ=60)

                                                                       CTGAAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCG                        >  SRR4302540.8535999/1‑94 (MQ=60)

                                                                          AAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGC                     >  SRR4302540.2329470/1‑94 (MQ=60)

                                                                          AAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGC                     >  SRR4302540.5736058/1‑94 (MQ=60)

                                                                          AAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGC                     >  SRR4302540.846847/1‑94 (MQ=60)

                                                                          AAAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGC                     >  SRR4302540.2329469/1‑94 (MQ=60)

                                                                           AAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCC                    <  SRR4302540.4128336/94‑1 (MQ=60)

                                                                           AAAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCC                    >  SRR4302540.1248869/1‑94 (MQ=60)

                                                                            AAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCG                   <  SRR4302540.1979557/94‑1 (MQ=60)

                                                                            AAGCTACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCG                   >  SRR4302540.1687830/1‑94 (MQ=60)

                                                                                TACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGG               <  SRR4302540.3120049/94‑1 (MQ=60)

                                                                                 ACTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGCTGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGCGCCGTTGGC              >  SRR4302540.3956035/1‑94 (MQ=60)

                                                                                  CTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCG             >  SRR4302540.8773186/1‑94 (MQ=60)

                                                                                  CTTCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCG             >  SRR4302540.4986940/1‑94 (MQ=60)

                                                                                  CATCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCG             <  SRR4302540.2465157/94‑1 (MQ=60)

                                                                                    TCGACGCCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGAT           <  SRR4302540.7371708/94‑1 (MQ=60)

                                                                                    TCGACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGAT           <  SRR4302540.9247248/94‑1 (MQ=60)

                                                                                      GACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCACATTATCTTCCGCATCACCGTGCCGTTGGAGATGG         >  SRR4302540.3952642/1‑94 (MQ=60)

                                                                                       ACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTC        <  SRR4302540.1599941/94‑1 (MQ=60)

                                                                                       ACACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTC        >  SRR4302540.8495652/1‑94 (MQ=60)

                                                                                         ACCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCG      >  SRR4302540.8184854/1‑94 (MQ=60)

                                                                                          CCACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGG     <  SRR4302540.7104972/94‑1 (MQ=60)

                                                                                           CACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGGG    <  SRR4302540.5211002/94‑1 (MQ=60)

                                                                                           CACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGGG    <  SRR4302540.2359419/94‑1 (MQ=60)

                                                                                           CACCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGGG    <  SRR4302540.8887781/94‑1 (MQ=60)

                                                                                             CCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGGGCT  <  SRR4302540.5132582/94‑1 (MQ=60)

                                                                                             CCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGGGCT  >  SRR4302540.8312374/1‑94 (MQ=60)

                                                                                             CCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGGGCT  <  SRR4302540.5886335/94‑1 (MQ=60)

                                                                                             CCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGGGCT  >  SRR4302540.4104674/1‑94 (MQ=60)

                                                                                             CCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGGGCT  >  SRR4302540.4104673/1‑94 (MQ=60)

GCATTTACGACACTGAGATGGCGCTGATCATCACCATGGTGACGCAAACTCTGCCCACCGCCGTGTTTATGCTGAAAAGCTACTTCGACACCATCCCCGATGAGATCGAAGAAGCGGCGATGATGGATGGCCTCAACCGTCTGCAAATTATCTTCCGCATCACCGTGCCGTTGGCGATGTCCGGGCT  >  NC_000913/1372523‑1372709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`