Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,376,771	C→T	G288G (GGC→GGT)	ycjT →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,376,771	0	C	T	99.6%	833.4 / ‑5.8	235	G288G (GGC→GGT)	ycjT	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (125/107); total (128/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 252 total aligned reads displayed.

GTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGCGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGC  >  NC_000913/1376681‑1376860

gtgCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAAtggtgg                                                                                         >  1:1856346/1‑93 (MQ=255)

gtgCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAAtggtgg                                                                                         <  1:4790827/93‑1 (MQ=255)

  gcgcgcAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGgtggcg                                                                                       <  1:908161/93‑1 (MQ=255)

  gcgcgcAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGATAAATGgtggcg                                                                                       >  1:3138866/1‑93 (MQ=255)

     cgcAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAg                                                                                    <  2:4321418/93‑1 (MQ=255)

     cgcAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAg                                                                                    <  2:7240566/93‑1 (MQ=255)

       cagcagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAgcg                                                                                  >  2:4677165/1‑93 (MQ=255)

       cagcagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAgcg                                                                                  <  2:6471661/93‑1 (MQ=255)

        agcagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAgcgc                                                                                 <  2:2410869/93‑1 (MQ=255)

        agcagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAgcgc                                                                                 <  2:5272078/93‑1 (MQ=255)

         gcagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCa                                                                                >  2:9388840/1‑93 (MQ=255)

          cagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCAc                                                                               >  2:1527039/1‑93 (MQ=255)

          cagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCAc                                                                               >  1:4441681/1‑93 (MQ=255)

          cagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCAc                                                                               >  1:3746962/1‑93 (MQ=255)

           agagTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGGAGCGCACg                                                                              >  1:4283940/1‑93 (MQ=255)

           agagTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACg                                                                              <  1:5631124/93‑1 (MQ=255)

           agagTTACGACCAACATATTGCAGAATCAACAGAAAACTGGAGTCAATGGTGGCAGAAACATCGTATCACGGTAAATGGTGGCGAAGCGCAca                                                                              <  1:8963222/93‑2 (MQ=255)

            gagTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGa                                                                             <  1:8091300/93‑1 (MQ=255)

            gagTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGa                                                                             >  2:1005032/1‑93 (MQ=255)

             atttACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGAt                                                                            <  2:4761333/91‑1 (MQ=255)

             agTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGAt                                                                            <  1:7040417/93‑1 (MQ=255)

             agTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGAt                                                                            <  2:2680567/93‑1 (MQ=255)

              gTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATc                                                                           <  1:7714628/93‑1 (MQ=255)

              gTTACGACCAACTTCTTGCAGAATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATc                                                                           >  1:2800746/1‑93 (MQ=255)

               ttACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCa                                                                          <  2:1762854/93‑1 (MQ=255)

                tACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAg                                                                         >  1:3997789/1‑93 (MQ=255)

                 aCGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGc                                                                        <  2:8232500/93‑1 (MQ=255)

                   gACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCaa                                                                      <  2:7083334/93‑1 (MQ=255)

                    aCCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAg                                                                     >  2:2348632/1‑93 (MQ=255)

                     ccAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGc                                                                    >  1:6301175/1‑93 (MQ=255)

                      cAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCg                                                                   >  1:2402179/1‑93 (MQ=255)

                      cAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGGGGCGAAGCGCACGATCAGCAAGCg                                                                   >  1:5806129/1‑93 (MQ=255)

                       aaCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATAAGCAAGCGt                                                                  >  1:1098634/1‑93 (MQ=255)

                        aCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGtt                                                                 >  2:5147588/1‑93 (MQ=255)

                        aCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGtt                                                                 <  1:1703836/93‑1 (MQ=255)

                         cttcttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTa                                                                <  2:9755637/93‑1 (MQ=255)

                         cttcttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTa                                                                >  1:5727596/1‑93 (MQ=255)

                            cttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGAt                                                             <  1:5007506/93‑1 (MQ=255)

                             ttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGAtt                                                            <  2:7593980/93‑1 (MQ=255)

                              tGCAGTATCAACCGAAAACTGGCGTCAATGGTGGCAGAAACGGCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTa                                                           <  1:8391137/93‑1 (MQ=255)

                                cagcaTCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATg                                                         <  2:1983942/93‑1 (MQ=255)

                                 agcaTCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATgc                                                        >  1:7278037/1‑93 (MQ=255)

                                 agcaTCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATgc                                                        <  2:5904449/93‑1 (MQ=255)

                                  gcaTCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATgcg                                                       >  2:7697184/1‑93 (MQ=255)

                                  gcaTCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATAAGCAAGCGTTAGATTATgcg                                                       >  2:2188151/1‑93 (MQ=255)

                                  gcaTCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGGAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATgcg                                                       >  2:3369992/1‑93 (MQ=255)

                                     tCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCtt                                                    >  2:3051656/1‑93 (MQ=255)

                                     tCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCtt                                                    >  2:2238683/1‑93 (MQ=255)

                                     tCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCtt                                                    <  2:4981453/93‑1 (MQ=255)

                                         cAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatc                                                >  1:8741038/1‑93 (MQ=255)

                                          aGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatca                                               <  1:4628645/93‑1 (MQ=255)

                                           gAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatcat                                              >  2:9472591/1‑93 (MQ=255)

                                           gAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatcat                                              >  1:3708535/1‑93 (MQ=255)

                                           gAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGG‑TGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatcctc                                             >  1:4512210/1‑93 (MQ=255)

                                            aaaaCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatcatc                                             <  2:8567012/93‑1 (MQ=255)

                                             aaaCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCt                                            >  1:2187726/1‑93 (MQ=255)

                                             aaaCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCt                                            <  2:3997491/93‑1 (MQ=255)

                                              aaCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTg                                           <  2:3907967/93‑1 (MQ=255)

                                               aCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTgc                                          <  1:3554365/93‑1 (MQ=255)

                                                 tGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTgcgc                                        <  2:8797486/93‑1 (MQ=255)

                                                   gCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGcat                                      >  2:6741185/1‑93 (MQ=255)

                                                    cGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGcatc                                     >  2:5958910/1‑93 (MQ=255)

                                                     gTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGcatca                                    <  2:2955899/93‑1 (MQ=255)

                                                      tCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGcatcat                                   <  1:6090232/93‑1 (MQ=255)

                                                      tCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGcatcat                                   >  1:5271348/1‑93 (MQ=255)

                                                       cAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATg                                  <  1:7882590/93‑1 (MQ=255)

                                                        aaTGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGa                                 >  1:7377375/1‑93 (MQ=255)

                                                        aaTGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGa                                 <  1:2807808/93‑1 (MQ=255)

                                                        aaTGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGa                                 <  2:4071931/93‑1 (MQ=255)

                                                        aaTGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGa                                 <  1:8202778/93‑1 (MQ=255)

                                                         aTGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGAc                                >  1:1427841/1‑93 (MQ=255)

                                                         aTGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGAc                                <  1:959933/93‑1 (MQ=255)

                                                            gtggCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGcc                             <  1:591058/93‑1 (MQ=255)

                                                            gtggCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGcc                             <  2:7792556/93‑1 (MQ=255)

                                                              ggCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTg                           >  2:698557/1‑93 (MQ=255)

                                                               gCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGc                          >  1:8806548/1‑93 (MQ=255)

                                                                  gAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCa                       >  2:3346423/1‑93 (MQ=255)

                                                                  gAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCa                       <  2:6203975/93‑1 (MQ=255)

                                                                  gAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCa                       >  2:2283342/1‑93 (MQ=255)

                                                                    aaCGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCacg                     >  2:926077/1‑93 (MQ=255)

                                                                     aCGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCacga                    <  1:9826808/93‑1 (MQ=255)

                                                                     aCGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCacga                    <  1:9352127/93‑1 (MQ=255)

                                                                     aCGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCCCATCATGTCGCCTGCCCacga                    <  2:1252998/93‑1 (MQ=255)

                                                                      cgtcgtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCacgac                   <  1:1170703/93‑1 (MQ=255)

                                                                       gtcgtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCacgacg                  >  1:7575925/1‑93 (MQ=255)

                                                                        tcgtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCAcgacga                 >  2:5099365/1‑93 (MQ=255)

                                                                          gtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAgc               <  2:8006098/93‑1 (MQ=255)

                                                                             tCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgca            >  1:7799235/1‑93 (MQ=255)

                                                                                cGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgcagca         <  2:3079507/93‑1 (MQ=255)

                                                                                cGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgcagca         <  1:887388/93‑1 (MQ=255)

                                                                                cGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgcagca         <  1:6803084/93‑1 (MQ=255)

                                                                                cGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgcagca         >  1:6632638/1‑93 (MQ=255)

                                                                                 ggTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgcaggag        >  2:2999981/1‑93 (MQ=255)

                                                                                 ggTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgcagcag        >  1:1942750/1‑93 (MQ=255)

                                                                                 ggTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTCCCCACGGCGCGCgcagcag        >  2:3024106/1‑93 (MQ=255)

                                                                                   tAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgcagcagca      <  2:4503108/93‑1 (MQ=255)

                                                                                   tAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgcagcagca      >  1:5477486/1‑93 (MQ=255)

                                                                                    aaaTGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCAt     <  1:9928623/93‑1 (MQ=255)

                                                                                       tggtggCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGc  >  2:6684499/1‑93 (MQ=255)

                                                                                       tggtggCGAAGCGCACGATCAGCAAGCGTTAGATCATGCGCTCTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGc  <  2:8625050/93‑1 (MQ=255)

GTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGCGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGC  >  NC_000913/1376681‑1376860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGCGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGGC  >  NC_000913/1376678‑1376863

AATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGT                                                                                              >  SRR4302540.6541813/1‑94 (MQ=60)

AATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGG                                                                                              >  SRR4302540.8441320/1‑94 (MQ=60)

 ATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTG                                                                                             >  SRR4302540.7170391/1‑94 (MQ=60)

 ATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTG                                                                                             <  SRR4302540.3725243/94‑1 (MQ=60)

 ATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTG                                                                                             <  SRR4302540.4780916/94‑1 (MQ=60)

 ATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTG                                                                                             <  SRR4302540.5915107/94‑1 (MQ=60)

 ATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTG                                                                                             >  SRR4302540.6371505/1‑94 (MQ=60)

  TGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGG                                                                                            >  SRR4302540.1876702/1‑94 (MQ=60)

   GTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGC                                                                                           <  SRR4302540.4842067/94‑1 (MQ=60)

   GTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGC                                                                                           <  SRR4302540.4842066/94‑1 (MQ=60)

    TGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGATAAATGGTGGCG                                                                                          >  SRR4302540.3173099/1‑94 (MQ=60)

     GCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGA                                                                                         <  SRR4302540.917793/94‑1 (MQ=60)

      CGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAA                                                                                        >  SRR4302540.3029460/1‑94 (MQ=60)

       GCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAG                                                                                       <  SRR4302540.772461/94‑1 (MQ=60)

          CAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGC                                                                                    <  SRR4302540.8638375/94‑1 (MQ=60)

          CAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGC                                                                                    >  SRR4302540.6402824/1‑94 (MQ=60)

            GCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCAC                                                                                  >  SRR4302540.7863666/1‑94 (MQ=60)

            GCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCAC                                                                                  >  SRR4302540.3787849/1‑94 (MQ=60)

            GCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCAC                                                                                  <  SRR4302540.5619515/94‑1 (MQ=60)

            GCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCAC                                                                                  >  SRR4302540.4489814/1‑94 (MQ=60)

             CAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGGAGCGCACG                                                                                 >  SRR4302540.4330540/1‑94 (MQ=60)

             CAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACG                                                                                 <  SRR4302540.9528172/94‑1 (MQ=60)

              AGAGTTACGACCAACTTCTTGCAGCATCACCAGAAAACTGGCGTCAATGGTGACAGAAACGTCGTATCACGGTAAATGGTGGCGACGCGCACAA                                                                                >  SRR4302540.7085301/1‑94 (MQ=60)

              AGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGA                                                                                <  SRR4302540.5693106/94‑1 (MQ=60)

              AGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGA                                                                                >  SRR4302540.7085302/1‑94 (MQ=60)

               GAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGAT                                                                               >  SRR4302540.4111588/1‑94 (MQ=60)

               GAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGAT                                                                               <  SRR4302540.8185216/94‑1 (MQ=60)

                AGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATC                                                                              >  SRR4302540.2830995/1‑94 (MQ=60)

                AGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATC                                                                              <  SRR4302540.7118692/94‑1 (MQ=60)

                AGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATC                                                                              <  SRR4302540.8134219/94‑1 (MQ=60)

                AGTTACGACCAACTTCTTGCAGAATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATC                                                                              >  SRR4302540.2830994/1‑94 (MQ=60)

                 GTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCA                                                                             <  SRR4302540.7803589/94‑1 (MQ=60)

                  TTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAG                                                                            >  SRR4302540.4040817/1‑94 (MQ=60)

                       ACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGC                                                                       >  SRR4302540.4605541/1‑94 (MQ=60)

                       ACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGC                                                                       >  SRR4302540.1239907/1‑94 (MQ=60)

                       ACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGC                                                                       >  SRR4302540.6371346/1‑94 (MQ=60)

                        CCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCG                                                                      <  SRR4302540.9958956/94‑1 (MQ=60)

                        CCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCG                                                                      >  SRR4302540.7937170/1‑94 (MQ=60)

                        CCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCG                                                                      >  SRR4302540.2428085/1‑94 (MQ=60)

                        CCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGGGGCGAAGCGCACGATCAGCAAGCG                                                                      >  SRR4302540.5870285/1‑94 (MQ=60)

                         CAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGT                                                                     >  SRR4302540.3691665/1‑94 (MQ=60)

                         CAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGT                                                                     >  SRR4302540.3691664/1‑94 (MQ=60)

                         CAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATAAGCAAGCGT                                                                     >  SRR4302540.1109947/1‑94 (MQ=60)

                           ACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTA                                                                   <  SRR4302540.1722268/94‑1 (MQ=60)

                           ACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTA                                                                   >  SRR4302540.5790670/1‑94 (MQ=60)

                            CTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAG                                                                  <  SRR4302540.4582542/94‑1 (MQ=60)

                            CTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAG                                                                  >  SRR4302540.5948651/1‑94 (MQ=60)

                            CTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAG                                                                  <  SRR4302540.8244461/94‑1 (MQ=60)

                               CTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACTGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATT                                                               >  SRR4302540.5757744/1‑94 (MQ=60)

                               CTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATT                                                               <  SRR4302540.5061738/94‑1 (MQ=60)

                               CTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATT                                                               <  SRR4302540.2552717/94‑1 (MQ=60)

                                TTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTA                                                              <  SRR4302540.9150025/94‑1 (MQ=60)

                                TGGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTA                                                              <  SRR4302540.9150026/94‑1 (MQ=60)

                                 TGCAGTATCAACCGAAAACTGGCGTCAATGGTGGCAGAAACGGCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTAT                                                             <  SRR4302540.8488507/94‑1 (MQ=60)

                                 TGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTAT                                                             <  SRR4302540.4990708/94‑1 (MQ=60)

                                  GCAGCATCAACAGAAAACTGGCGTCAATGGTGGCTGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATG                                                            <  SRR4302540.1968567/94‑1 (MQ=60)

                                   CAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGC                                                           >  SRR4302540.7359243/1‑94 (MQ=60)

                                   CAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGC                                                           >  SRR4302540.5308254/1‑94 (MQ=60)

                                    AGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCG                                                          >  SRR4302540.9240657/1‑94 (MQ=60)

                                     GCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGC                                                         >  SRR4302540.4358069/1‑94 (MQ=60)

                                       ATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTT                                                       >  SRR4302540.1768029/1‑94 (MQ=60)

                                       ATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTT                                                       >  SRR4302540.1151342/1‑94 (MQ=60)

                                       ATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTT                                                       >  SRR4302540.10113846/1‑94 (MQ=60)

                                       ATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTT                                                       >  SRR4302540.8922074/1‑94 (MQ=60)

                                         CAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTA                                                     >  SRR4302540.3485857/1‑94 (MQ=60)

                                          AACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTAT                                                    >  SRR4302540.1922382/1‑94 (MQ=60)

                                          AACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTAT                                                    >  SRR4302540.5779972/1‑94 (MQ=60)

                                          AACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTAT                                                    >  SRR4302540.2419092/1‑94 (MQ=60)

                                          AACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTAT                                                    <  SRR4302540.607970/94‑1 (MQ=60)

                                           ACAGAAAACTGGTGTCAATGGTGGTAGACACGTCGTATCGCGGTAAATGGTGGCGAGGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATC                                                   <  SRR4302540.8926550/94‑1 (MQ=60)

                                           ACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATC                                                   >  SRR4302540.8842507/1‑94 (MQ=60)

                                            CAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTAAGCGCTTTATCA                                                  >  SRR4302540.4367509/1‑94 (MQ=60)

                                             AGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCAT                                                 >  SRR4302540.3749008/1‑94 (MQ=60)

                                             AGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCAT                                                 <  SRR4302540.4678229/94‑1 (MQ=60)

                                             AGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGG‑TGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCCTC                                                >  SRR4302540.4561100/1‑94 (MQ=60)

                                               AAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCT                                               >  SRR4302540.10113288/1‑94 (MQ=60)

                                               AAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCT                                               >  SRR4302540.2211345/1‑94 (MQ=60)

                                                AAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTG                                              <  SRR4302540.7140219/94‑1 (MQ=60)

                                                  ACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCG                                            <  SRR4302540.3593200/94‑1 (MQ=60)

                                                    TGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCA                                          >  SRR4302540.3997935/1‑94 (MQ=60)

                                                     GGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCAT                                         <  SRR4302540.8338215/94‑1 (MQ=60)

                                                      GCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATC                                        >  SRR4302540.6354125/1‑94 (MQ=60)

                                                        GTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCAT                                      >  SRR4302540.5328890/1‑94 (MQ=60)

                                                        GTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCAT                                      >  SRR4302540.5328888/1‑94 (MQ=60)

                                                        GTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCAT                                      >  SRR4302540.9713476/1‑94 (MQ=60)

                                                         TCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATG                                     <  SRR4302540.6158012/94‑1 (MQ=60)

                                                          CAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGA                                    >  SRR4302540.7459587/1‑94 (MQ=60)

                                                          CAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGA                                    <  SRR4302540.7974629/94‑1 (MQ=60)

                                                           AATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGAC                                   <  SRR4302540.2838132/94‑1 (MQ=60)

                                                           AATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGAC                                   <  SRR4302540.8298101/94‑1 (MQ=60)

                                                           AATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGAC                                   >  SRR4302540.1443300/1‑94 (MQ=60)

                                                            ATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACG                                  <  SRR4302540.4734458/94‑1 (MQ=60)

                                                            ATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACG                                  <  SRR4302540.969869/94‑1 (MQ=60)

                                                             TGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGC                                 <  SRR4302540.3997951/94‑1 (MQ=60)

                                                             TGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGC                                 >  SRR4302540.1620396/1‑94 (MQ=60)

                                                              GGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCC                                <  SRR4302540.8916177/94‑1 (MQ=60)

                                                              GGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCC                                <  SRR4302540.2820403/94‑1 (MQ=60)

                                                               GTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCT                               <  SRR4302540.5857449/94‑1 (MQ=60)

                                                               GTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCT                               <  SRR4302540.597427/94‑1 (MQ=60)

                                                               GTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCT                               <  SRR4302540.7891806/94‑1 (MQ=60)

                                                               GTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCT                               >  SRR4302540.1748753/1‑94 (MQ=60)

                                                                TGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTG                              <  SRR4302540.8231/94‑1 (MQ=60)

                                                                 GGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGC                             >  SRR4302540.8908358/1‑94 (MQ=60)

                                                                    AGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCC                          >  SRR4302540.643379/1‑94 (MQ=60)

                                                                    AGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCA                          <  SRR4302540.2931332/94‑1 (MQ=60)

                                                                     GAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCAC                         <  SRR4302540.7480699/94‑1 (MQ=60)

                                                                     GAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCAC                         <  SRR4302540.5474612/94‑1 (MQ=60)

                                                                       AACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGA                       >  SRR4302540.6039227/1‑94 (MQ=60)

                                                                        ACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGAC                      <  SRR4302540.9463510/94‑1 (MQ=60)

                                                                        ACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGAC                      <  SRR4302540.9941761/94‑1 (MQ=60)

                                                                         CGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACG                     <  SRR4302540.1182825/94‑1 (MQ=60)

                                                                         CGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACG                     >  SRR4302540.7660931/1‑94 (MQ=60)

                                                                         CGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACG                     >  SRR4302540.7660930/1‑94 (MQ=60)

                                                                         CGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACG                     <  SRR4302540.8897099/94‑1 (MQ=60)

                                                                          GTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGA                    >  SRR4302540.5838122/1‑94 (MQ=60)

                                                                           TCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACAACGAG                   >  SRR4302540.871380/1‑94 (MQ=60)

                                                                              TATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGC                <  SRR4302540.7388272/94‑1 (MQ=60)

                                                                               ATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCA               >  SRR4302540.7890401/1‑94 (MQ=60)

                                                                                  ACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCA            <  SRR4302540.7414576/94‑1 (MQ=60)

                                                                                  ACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCA            >  SRR4302540.6706051/1‑94 (MQ=60)

                                                                                   CGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAG           >  SRR4302540.1963903/1‑94 (MQ=60)

                                                                                   CGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAG           <  SRR4302540.6878354/94‑1 (MQ=60)

                                                                                   CGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAG           <  SRR4302540.896809/94‑1 (MQ=60)

                                                                                    GGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGC          <  SRR4302540.9944621/94‑1 (MQ=60)

                                                                                     GTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCA         >  SRR4302540.5537779/1‑94 (MQ=60)

                                                                                     GTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCA         >  SRR4302540.6860373/1‑94 (MQ=60)

                                                                                      TAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCAT        <  SRR4302540.1234294/94‑1 (MQ=60)

                                                                                       AAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATT       <  SRR4302540.10044530/94‑1 (MQ=60)

                                                                                       AAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATT       >  SRR4302540.3749932/1‑94 (MQ=60)

                                                                                       AAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATT       >  SRR4302540.4542315/1‑94 (MQ=60)

                                                                                       AAATGGCGGCGAAGCGCACGATCAGCAAGCGTTTGATTATGCGCTTTATCATCTGCGCATCATGACGCCCGCCCACGATGAGCGCAGCAGCATT       >  SRR4302540.9052295/1‑94 (MQ=60)

                                                                                         ATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCATCATTGC     >  SRR4302540.9671051/1‑94 (MQ=60)

                                                                                         ATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGC     >  SRR4302540.2018798/1‑94 (MQ=60)

                                                                                           GGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGG   <  SRR4302540.6369075/94‑1 (MQ=60)

                                                                                            GTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGGC  <  SRR4302540.9193742/94‑1 (MQ=60)

                                                                                            GTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGGC  >  SRR4302540.10133143/1‑94 (MQ=60)

                                                                                            GTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGGC  >  SRR4302540.10133142/1‑94 (MQ=60)

                                                                                            GTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGGC  >  SRR4302540.349403/1‑94 (MQ=60)

AATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGCGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGGC  >  NC_000913/1376678‑1376863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`