Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,376,771 C→T G288G (GGC→GGT)  ycjT → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,7710CT99.5% 720.6 / ‑5.4 201G288G (GGC→GGT) ycjThypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (93/107);  total (94/107)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

Only 100 of 211 total aligned reads displayed.

GTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGCGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGC  >  NC_000913/1376681‑1376860
                                                                                          |                                                                                         
gtgCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAAtggtgg                                                                                         <  1:5438009/93‑1 (MQ=255)
 tgCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATggtggc                                                                                        <  1:1566146/93‑1 (MQ=255)
   cgcgcAGCAGAGTTACGACCAGCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGa                                                                                      <  2:1567230/93‑1 (MQ=255)
    gcgcAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGaa                                                                                     >  2:2204015/1‑93 (MQ=255)
     cgcAGCAGAGTTACGACCATCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAg                                                                                    <  1:5361428/93‑1 (MQ=255)
       cagcagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAgcg                                                                                  <  2:5749374/93‑1 (MQ=255)
        agcagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAgcgc                                                                                 >  1:5600688/1‑93 (MQ=255)
        agcagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGCCAGAAACGTCGTATCACGGTAAATGGTGGCGAAgcgc                                                                                 >  2:3928407/1‑93 (MQ=255)
         gcagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCa                                                                                >  1:2100829/1‑93 (MQ=255)
         gcagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCa                                                                                >  1:3668571/1‑93 (MQ=255)
          cagAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCAc                                                                               <  2:4437043/93‑1 (MQ=255)
           agagTTACGACCAGCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACg                                                                              <  2:122599/93‑1 (MQ=255)
            gagTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGa                                                                             >  2:1897460/1‑93 (MQ=255)
             agTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGAt                                                                            <  2:4967835/93‑1 (MQ=255)
             agTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGAt                                                                            <  2:2151757/93‑1 (MQ=255)
              gTTACGACCCACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATc                                                                           <  2:5049093/93‑1 (MQ=255)
              gTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATc                                                                           <  1:4452691/93‑1 (MQ=255)
                tACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAg                                                                         <  2:5415128/93‑1 (MQ=255)
                   gACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCaa                                                                      >  1:946475/1‑93 (MQ=255)
                   gACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCaa                                                                      >  1:1913154/1‑93 (MQ=255)
                    aCCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAg                                                                     >  1:572154/1‑93 (MQ=255)
                    aCCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAg                                                                     >  1:3306982/1‑93 (MQ=255)
                     ccAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCATGATCAGCAAGc                                                                    <  2:4237127/93‑1 (MQ=255)
                     ccAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGc                                                                    <  1:379162/93‑1 (MQ=255)
                     ccAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGc                                                                    <  2:1627316/93‑1 (MQ=255)
                     ccAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGc                                                                    >  2:3756505/1‑93 (MQ=255)
                     ccAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGc                                                                    >  2:4104387/1‑93 (MQ=255)
                      cAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCg                                                                   >  1:3945894/1‑93 (MQ=255)
                      cAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCg                                                                   >  1:327294/1‑93 (MQ=255)
                       aaCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGt                                                                  >  2:3631471/1‑93 (MQ=255)
                       aaCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGt                                                                  >  2:3303859/1‑93 (MQ=255)
                       aaCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGt                                                                  >  2:805051/1‑93 (MQ=255)
                        aCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGtt                                                                 <  2:395365/93‑1 (MQ=255)
                         cttcttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTa                                                                <  1:3675950/93‑1 (MQ=255)
                         cttcttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTa                                                                <  1:3061105/93‑1 (MQ=255)
                           tcttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGa                                                              <  2:357675/93‑1 (MQ=255)
                           tcttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGa                                                              <  2:2032887/93‑1 (MQ=255)
                             ttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGAtt                                                            >  1:4423198/1‑93 (MQ=255)
                             ttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGAtt                                                            >  2:3356307/1‑93 (MQ=255)
                             ttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGAtt                                                            >  2:5961248/1‑93 (MQ=255)
                             ttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGAtt                                                            <  2:4531061/93‑1 (MQ=255)
                             ttGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGAtt                                                            <  2:2308197/93‑1 (MQ=255)
                              tGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTa                                                           <  1:368773/93‑1 (MQ=255)
                              tGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTa                                                           <  2:3908310/93‑1 (MQ=255)
                              tGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTa                                                           <  2:4031393/93‑1 (MQ=255)
                                cagcaTCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATg                                                         <  2:3088947/93‑1 (MQ=255)
                                cagcaTCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATg                                                         >  1:2977535/1‑93 (MQ=255)
                                   gaTCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATgcgc                                                      <  1:5673700/92‑1 (MQ=255)
                                     tCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCtt                                                    >  2:3229963/1‑93 (MQ=255)
                                      cAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCttt                                                   <  2:1281054/93‑1 (MQ=255)
                                       aaCAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTa                                                  <  1:1220387/93‑1 (MQ=255)
                                       aaCAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTa                                                  <  2:6176849/93‑1 (MQ=255)
                                         cAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatc                                                >  2:4685415/1‑93 (MQ=255)
                                         cAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatc                                                <  2:4290771/93‑1 (MQ=255)
                                         cAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatc                                                >  1:5813512/1‑93 (MQ=255)
                                          aGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatca                                               <  2:1454863/93‑1 (MQ=255)
                                          aGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatca                                               <  1:2754918/93‑1 (MQ=255)
                                          aGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatca                                               >  1:3630305/1‑93 (MQ=255)
                                          aGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatca                                               <  2:3547346/93‑1 (MQ=255)
                                           gAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatcat                                              >  1:2921307/1‑93 (MQ=255)
                                            aaaaCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAANCGTTAGATTATGCGCTTTatcatc                                             >  2:650632/1‑93 (MQ=255)
                                            aaaaCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTatcatc                                             <  2:1129781/93‑1 (MQ=255)
                                              aaCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTg                                           >  1:6052748/1‑93 (MQ=255)
                                              aaCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTg                                           >  1:3651099/1‑93 (MQ=255)
                                              aaCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTg                                           >  2:4866102/1‑93 (MQ=255)
                                              aaCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTg                                           >  2:2627634/1‑93 (MQ=255)
                                               aCTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTgc                                          >  2:3844973/1‑93 (MQ=255)
                                                cTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGTGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTgcg                                         <  1:5025752/93‑1 (MQ=255)
                                                 tGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTgcgc                                        >  2:5003815/1‑93 (MQ=255)
                                                   gCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGcat                                      <  2:4726456/93‑1 (MQ=255)
                                                     gTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGcatca                                    <  1:4842954/93‑1 (MQ=255)
                                                       cAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATg                                  <  2:4812639/93‑1 (MQ=255)
                                                        aaTGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCCGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGa                                 <  1:2220387/93‑1 (MQ=255)
                                                             tggCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCt                            <  2:5223384/93‑1 (MQ=255)
                                                               gCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGc                          >  2:3018099/1‑93 (MQ=255)
                                                                 aGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGccc                        <  2:1346517/93‑1 (MQ=255)
                                                                 aGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGccc                        <  1:318001/93‑1 (MQ=255)
                                                                  gAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCa                       <  2:5593263/93‑1 (MQ=255)
                                                                  gAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTGAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCa                       <  1:3374105/93‑1 (MQ=255)
                                                                     aCGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCacga                    >  1:2546149/1‑93 (MQ=255)
                                                                      cgtcgtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCacgac                   <  1:6305578/93‑1 (MQ=255)
                                                                      cgtcgtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCacgac                   <  1:1947156/93‑1 (MQ=255)
                                                                       gtcgtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCacgacg                  >  1:3724895/1‑93 (MQ=255)
                                                                       gtcgtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCacgacg                  >  2:4762496/1‑93 (MQ=255)
                                                                        tcgtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCAcgacga                 <  1:773054/93‑1 (MQ=255)
                                                                        tcgtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCAcgacga                 >  2:2534121/1‑93 (MQ=255)
                                                                          gtATCACGGTAAATGGTGGCGCAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCCcgacgacg               >  1:1613098/1‑91 (MQ=255)
                                                                          gtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAgc               <  1:4667943/93‑1 (MQ=255)
                                                                          gtATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAgc               >  1:1517277/1‑93 (MQ=255)
                                                                           tATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAgcg              >  1:2875018/1‑93 (MQ=255)
                                                                            aTCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAgcgc             >  2:2437543/1‑93 (MQ=255)
                                                                             tCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgca            <  1:3518267/93‑1 (MQ=255)
                                                                                cGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgcagca         >  1:5733309/1‑93 (MQ=255)
                                                                                   tAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCgcagcagca      <  1:1035676/93‑1 (MQ=255)
                                                                                    aaaTGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCAt     >  1:755037/1‑93 (MQ=255)
                                                                                    aaaTGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCAt     <  1:4291136/93‑1 (MQ=255)
                                                                                    aaaTGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCAt     >  2:1819855/1‑93 (MQ=255)
                                                                                      aTGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTg   >  1:5273057/1‑93 (MQ=255)
                                                                                       tggtggCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGc  <  2:5938298/93‑1 (MQ=255)
                                                                                       tggtggCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGc  <  2:1533354/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGCGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGC  >  NC_000913/1376681‑1376860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGCGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGGC  >  NC_000913/1376679‑1376863
                                                                                            |                                                                                            
ATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTG                                                                                             >  SRR4302533.1668778/1‑94 (MQ=60)
 TGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGG                                                                                            <  SRR4302533.4282625/94‑1 (MQ=60)
 TGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGG                                                                                            <  SRR4302533.681704/94‑1 (MQ=60)
 TGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGG                                                                                            <  SRR4302533.2066300/94‑1 (MQ=60)
 TGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGG                                                                                            <  SRR4302533.569907/94‑1 (MQ=60)
  GTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGC                                                                                           <  SRR4302533.5470763/94‑1 (MQ=60)
  GTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGC                                                                                           <  SRR4302533.6075013/94‑1 (MQ=60)
   TGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCG                                                                                          <  SRR4302533.1575576/94‑1 (MQ=60)
     CGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAA                                                                                        <  SRR4302533.4069591/94‑1 (MQ=60)
      GCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAG                                                                                       <  SRR4302533.3307071/94‑1 (MQ=60)
       CGCAGCAGAGTTACGACCATCTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGC                                                                                      <  SRR4302533.5393628/94‑1 (MQ=60)
         CAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGC                                                                                    >  SRR4302533.5634527/1‑94 (MQ=60)
          AGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCA                                                                                   >  SRR4302533.3691118/1‑94 (MQ=60)
          AGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCA                                                                                   >  SRR4302533.2113786/1‑94 (MQ=60)
          AGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCA                                                                                   >  SRR4302533.675925/1‑94 (MQ=60)
            CAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACG                                                                                 <  SRR4302533.1588696/94‑1 (MQ=60)
             AGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGA                                                                                >  SRR4302533.369334/1‑94 (MQ=60)
             AGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGA                                                                                >  SRR4302533.3434077/1‑94 (MQ=60)
              GAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGAT                                                                               <  SRR4302533.5404232/94‑1 (MQ=60)
               AGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATC                                                                              >  SRR4302533.3553389/1‑94 (MQ=60)
               AGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATC                                                                              >  SRR4302533.88834/1‑94 (MQ=60)
                GTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCA                                                                             >  SRR4302533.3969603/1‑94 (MQ=60)
                GTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCA                                                                             <  SRR4302533.4479613/94‑1 (MQ=60)
                    CGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAA                                                                         >  SRR4302533.1924829/1‑94 (MQ=60)
                    CGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAA                                                                         >  SRR4302533.952039/1‑94 (MQ=60)
                     GACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGTTGGCAGAAACGTCGTATCGCGGTAAATGGTGGCGAAGCGCACGATCAGCAAG                                                                        <  SRR4302533.766867/94‑1 (MQ=60)
                     GACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAG                                                                        >  SRR4302533.575531/1‑94 (MQ=60)
                     GACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAG                                                                        >  SRR4302533.3327404/1‑94 (MQ=60)
                       CCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCG                                                                      <  SRR4302533.381447/94‑1 (MQ=60)
                       CCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCG                                                                      >  SRR4302533.329284/1‑94 (MQ=60)
                       CCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCG                                                                      >  SRR4302533.3969957/1‑94 (MQ=60)
                         AACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTT                                                                    >  SRR4302533.5760828/1‑94 (MQ=60)
                         AACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTT                                                                    <  SRR4302533.4421561/94‑1 (MQ=60)
                         AACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTT                                                                    >  SRR4302533.55268/1‑94 (MQ=60)
                         AACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTT                                                                    <  SRR4302533.6101239/94‑1 (MQ=60)
                           CTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAG                                                                  <  SRR4302533.3079891/94‑1 (MQ=60)
                           CTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAG                                                                  <  SRR4302533.3065563/94‑1 (MQ=60)
                           CTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAG                                                                  <  SRR4302533.3779138/94‑1 (MQ=60)
                           CTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAG                                                                  <  SRR4302533.3698541/94‑1 (MQ=60)
                            TTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGA                                                                 >  SRR4302533.3703637/1‑94 (MQ=60)
                              CTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATT                                                               >  SRR4302533.4449939/1‑94 (MQ=60)
                              CTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATT                                                               <  SRR4302533.4760247/94‑1 (MQ=60)
                              CTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATT                                                               <  SRR4302533.5049158/94‑1 (MQ=60)
                                TGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTAT                                                             <  SRR4302533.5502028/94‑1 (MQ=60)
                                TGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTAT                                                             <  SRR4302533.371000/94‑1 (MQ=60)
                                TGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTAT                                                             <  SRR4302533.1113476/94‑1 (MQ=60)
                                TGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTAT                                                             <  SRR4302533.3165467/94‑1 (MQ=60)
                                TGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTAT                                                             <  SRR4302533.192775/94‑1 (MQ=60)
                                 GCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATG                                                            <  SRR4302533.896882/94‑1 (MQ=60)
                                 GCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATG                                                            >  SRR4302533.2995753/1‑94 (MQ=60)
                                     GATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCT                                                        <  SRR4302533.5707965/94‑1 (MQ=60)
                                     CATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCT                                                        >  SRR4302533.5788603/1‑94 (MQ=60)
                                     CATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCT                                                        >  SRR4302533.323707/1‑94 (MQ=60)
                                        CAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTA                                                     <  SRR4302533.2708559/94‑1 (MQ=60)
                                         AACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTAT                                                    <  SRR4302533.1227712/94‑1 (MQ=60)
                                         AACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTAT                                                    >  SRR4302533.3689458/1‑94 (MQ=60)
                                          ACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATC                                                   >  SRR4302533.5848623/1‑94 (MQ=60)
                                           CAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCA                                                  >  SRR4302533.3652640/1‑94 (MQ=60)
                                           CAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCA                                                  <  SRR4302533.5378929/94‑1 (MQ=60)
                                            AGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCAT                                                 <  SRR4302533.2771812/94‑1 (MQ=60)
                                            AGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCAT                                                 >  SRR4302533.2939200/1‑94 (MQ=60)
                                               AAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTG                                              >  SRR4302533.6089407/1‑94 (MQ=60)
                                               AAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTG                                              <  SRR4302533.2065397/94‑1 (MQ=60)
                                               AAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTG                                              >  SRR4302533.3673547/1‑94 (MQ=60)
                                                  CTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGTGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGC                                           <  SRR4302533.5056185/94‑1 (MQ=60)
                                                    GGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCAT                                         >  SRR4302533.5095273/1‑94 (MQ=60)
                                                      CGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCA                                       <  SRR4302533.2734757/94‑1 (MQ=60)
                                                       GTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCAT                                      <  SRR4302533.4872306/94‑1 (MQ=60)
                                                        TCAATGGTGGCAGAAACGTCGTAACACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTCTACCATCTGCGCATCATA                                     >  SRR4302533.1883024/1‑94 (MQ=60)
                                                          AATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCCGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGAC                                   <  SRR4302533.2234094/94‑1 (MQ=60)
                                                          AATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGAC                                   <  SRR4302533.3455221/94‑1 (MQ=60)
                                                          AATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGAC                                   <  SRR4302533.3743249/94‑1 (MQ=60)
                                                          AATGGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGAC                                   <  SRR4302533.3374895/94‑1 (MQ=60)
                                                             GGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCC                                <  SRR4302533.1391956/94‑1 (MQ=60)
                                                             GGTGGCAGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCC                                >  SRR4302533.4516160/1‑94 (MQ=60)
                                                                   AGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCA                          >  SRR4302533.3662390/1‑94 (MQ=60)
                                                                   AGAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCA                          <  SRR4302533.319951/94‑1 (MQ=60)
                                                                    GAAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTGAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCAC                         <  SRR4302533.3394941/94‑1 (MQ=60)
                                                                     AAACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACG                        <  SRR4302533.4452817/94‑1 (MQ=60)
                                                                      AACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGA                       >  SRR4302533.2561815/1‑94 (MQ=60)
                                                                      AACGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGA                       >  SRR4302533.2813682/1‑94 (MQ=60)
                                                                        CGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACG                     <  SRR4302533.6343315/94‑1 (MQ=60)
                                                                        CGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACG                     >  SRR4302533.3747747/1‑94 (MQ=60)
                                                                        CGTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACG                     <  SRR4302533.1959027/94‑1 (MQ=60)
                                                                         GTCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGA                    >  SRR4302533.154326/1‑94 (MQ=60)
                                                                          TCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAG                   <  SRR4302533.2927575/94‑1 (MQ=60)
                                                                          TCGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAG                   <  SRR4302533.777599/94‑1 (MQ=60)
                                                                           CGTATCACGGTAAATGGTGGCGCAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCCCGACGAcg                  >  SRR4302533.1622875/1‑92 (MQ=60)
                                                                           CGTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGC                  >  SRR4302533.1526411/1‑94 (MQ=60)
                                                                            GTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCG                 <  SRR4302533.4696226/94‑1 (MQ=60)
                                                                            GTATCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCG                 >  SRR4302533.2892708/1‑94 (MQ=60)
                                                                               TCACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAG              <  SRR4302533.3539957/94‑1 (MQ=60)
                                                                                CACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGC             <  SRR4302533.2277438/94‑1 (MQ=60)
                                                                                 ACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCA            >  SRR4302533.5767922/1‑94 (MQ=60)
                                                                                 ACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCA            <  SRR4302533.2993693/94‑1 (MQ=60)
                                                                                 ACGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCA            <  SRR4302533.2146374/94‑1 (MQ=60)
                                                                                  CGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAG           >  SRR4302533.4076803/1‑94 (MQ=60)
                                                                                  CGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAG           >  SRR4302533.5731066/1‑94 (MQ=60)
                                                                                  CGGTAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAG           <  SRR4302533.5676987/94‑1 (MQ=60)
                                                                                     TAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCAT        >  SRR4302533.759480/1‑94 (MQ=60)
                                                                                     TAAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCAT        <  SRR4302533.1041765/94‑1 (MQ=60)
                                                                                      AAATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATT       <  SRR4302533.4316942/94‑1 (MQ=60)
                                                                                       AATGGTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTG      >  SRR4302533.5304676/1‑94 (MQ=60)
                                                                                           GTGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGGC  >  SRR4302533.657510/1‑94 (MQ=60)
                                                                                            |                                                                                            
ATGTGCGCGCAGCAGAGTTACGACCAACTTCTTGCAGCATCAACAGAAAACTGGCGTCAATGGTGGCAGAAACGTCGTATCACGGTAAATGGCGGCGAAGCGCACGATCAGCAAGCGTTAGATTATGCGCTTTATCATCTGCGCATCATGACGCCTGCCCACGACGAGCGCAGCAGCATTGCGGC  >  NC_000913/1376679‑1376863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑