Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,400,388	A→G	S298P (TCC→CCC)	b1337 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,400,388	0	A	G	100.0%	783.1 / NA	239	S298P (TCC→CCC)	b1337	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (110/129); total (110/129)

Only 100 of 246 total aligned reads displayed.

CCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCT  >  NC_000913/1400298‑1400477

 cAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACa                                                                                        <  2:9724715/93‑1 (MQ=255)

  aGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAg                                                                                       >  1:512572/1‑93 (MQ=255)

  aGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACGGGGACAg                                                                                       >  2:8924712/1‑93 (MQ=255)

   gTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAgg                                                                                      >  1:1038284/1‑93 (MQ=255)

    ttcttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGc                                                                                     >  2:3718899/1‑93 (MQ=255)

     tcttcGGAGTTCCATTCCGGGGGACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCt                                                                                    >  1:3271918/1‑93 (MQ=255)

      tttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCtt                                                                                   <  2:7789845/92‑1 (MQ=255)

      cttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCtt                                                                                   <  2:5405125/93‑1 (MQ=255)

       ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  >  1:6803170/1‑93 (MQ=255)

       ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  >  2:8512365/1‑93 (MQ=255)

       ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  >  2:2063181/1‑93 (MQ=255)

       ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  >  2:9618662/1‑93 (MQ=255)

        tcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTg                                                                                 <  1:5496858/93‑1 (MQ=255)

         cGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGt                                                                                <  2:1944993/93‑1 (MQ=255)

           gAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCg                                                                              >  2:8083367/1‑93 (MQ=255)

             gtcccATGCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGaa                                                                            <  1:9095338/90‑1 (MQ=255)

             gTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGaa                                                                            <  1:7767024/93‑1 (MQ=255)

              ttCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAg                                                                           >  1:6112904/1‑93 (MQ=255)

              ttCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAg                                                                           >  2:7903532/1‑93 (MQ=255)

               tCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGc                                                                          >  2:6494395/1‑93 (MQ=255)

               tCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGc                                                                          <  1:6349911/93‑1 (MQ=255)

                ccATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCg                                                                         >  2:5607345/1‑93 (MQ=255)

                 cATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCgg                                                                        <  2:4211640/93‑1 (MQ=255)

                   ttCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCa                                                                      >  2:1633952/1‑93 (MQ=255)

                    tCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCAt                                                                     <  2:5077813/93‑1 (MQ=255)

                    tCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCAt                                                                     >  2:3057235/1‑93 (MQ=255)

                      cGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTc                                                                   <  2:803309/93‑1 (MQ=255)

                      cGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTc                                                                   <  1:7002483/93‑1 (MQ=255)

                          gTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAAcc                                                               >  1:5431672/1‑93 (MQ=255)

                          gTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAAcc                                                               <  2:756884/93‑1 (MQ=255)

                              cAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTgg                                                           <  1:1719359/93‑1 (MQ=255)

                               aaaaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGt                                                          <  2:4777199/93‑1 (MQ=255)

                               aaaaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGt                                                          <  2:6227437/93‑1 (MQ=255)

                               aaaaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGt                                                          >  1:4042202/1‑93 (MQ=255)

                                 agtGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGttt                                                        <  2:4433646/91‑1 (MQ=255)

                                  aTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTc                                                       <  2:3879234/93‑1 (MQ=255)

                                  aTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTc                                                       <  1:1222993/93‑1 (MQ=255)

                                  aTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTc                                                       >  1:7161304/1‑93 (MQ=255)

                                   tGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCg                                                      >  2:6097291/1‑93 (MQ=255)

                                    gggATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCgg                                                     >  2:9313285/1‑93 (MQ=255)

                                     ggATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGt                                                    <  1:499720/93‑1 (MQ=255)

                                      gATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtc                                                   >  1:3391543/1‑93 (MQ=255)

                                       aTAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtca                                                  <  2:674946/93‑1 (MQ=255)

                                       aTAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtca                                                  >  1:7953027/1‑93 (MQ=255)

                                        tAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtcat                                                 <  2:2256127/93‑1 (MQ=255)

                                        tAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtcat                                                 >  2:1094966/1‑93 (MQ=255)

                                         aGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtcatc                                                <  1:3361381/93‑1 (MQ=255)

                                          gggCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtcatca                                               >  2:2433733/1‑93 (MQ=255)

                                           ggCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCaa                                              <  1:8242312/93‑1 (MQ=255)

                                           ggCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCaa                                              <  1:4382324/93‑1 (MQ=255)

                                           ggCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCaa                                              <  2:6205517/93‑1 (MQ=255)

                                           ggCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCaa                                              >  2:6438046/1‑93 (MQ=255)

                                           ggCCTGGGACATGGCATTTTCGAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCaa                                              <  1:505836/93‑1 (MQ=255)

                                            gCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAt                                             >  1:2890716/1‑93 (MQ=255)

                                            gCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAt                                             <  2:4539153/93‑1 (MQ=255)

                                            gCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAt                                             >  1:4861369/1‑93 (MQ=255)

                                               tGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGcc                                          <  1:6713206/93‑1 (MQ=255)

                                               tGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGcc                                          <  2:5703593/93‑1 (MQ=255)

                                               tGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGcc                                          >  2:689553/1‑93 (MQ=255)

                                               tGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGcc                                          >  2:7015491/1‑93 (MQ=255)

                                                   aCATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCAc                                      >  2:8326781/1‑93 (MQ=255)

                                                    cATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCAcc                                     >  1:1992052/1‑93 (MQ=255)

                                                     gtGGCATTGGTTAAGGTGCGATTCGGGAGATAACTGGGACAGGCGTTTTCGAAGCGGAATTCAACCGTGGTTTCGGTCATCAATGCCGCACCt                                    <  2:7524527/92‑1 (MQ=255)

                                                     aTGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCt                                    >  1:9863094/1‑93 (MQ=255)

                                                     aTGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCt                                    >  1:8110152/1‑93 (MQ=255)

                                                     aTGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCt                                    >  1:7394851/1‑93 (MQ=255)

                                                      tGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCtt                                   >  1:74932/1‑93 (MQ=255)

                                                      tGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCtt                                   >  2:1298815/1‑93 (MQ=255)

                                                       ggCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTc                                  <  1:7218391/93‑1 (MQ=255)

                                                       ggCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTc                                  >  1:5607389/1‑93 (MQ=255)

                                                       ggCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACATTc                                  >  1:9292426/1‑93 (MQ=255)

                                                          aTTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGc                               <  2:921794/93‑1 (MQ=255)

                                                           ttttCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCg                              >  1:9655421/1‑93 (MQ=255)

                                                            tttCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGa                             <  1:8459260/93‑1 (MQ=255)

                                                               cTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCTGCACCTTCGGCGAttt                          >  1:8989062/1‑93 (MQ=255)

                                                               cTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAttt                          >  2:1401177/1‑93 (MQ=255)

                                                               cTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAttt                          >  1:7847671/1‑93 (MQ=255)

                                                                tAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAtttt                         <  1:8605093/93‑1 (MQ=255)

                                                                tAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAtttt                         <  2:486074/93‑1 (MQ=255)

                                                                tAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAtttt                         >  1:9007564/1‑93 (MQ=255)

                                                                 aaGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTg                        <  2:1748363/93‑1 (MQ=255)

                                                                  aGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGGGATTTTgg                       >  2:8575067/1‑93 (MQ=255)

                                                                   ggTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGc                      >  2:5478767/1‑93 (MQ=255)

                                                                   ggTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGc                      >  1:1275432/1‑93 (MQ=255)

                                                                         aTTCGGGAGATAACTGGGACAGACTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTTGCGACCCg                >  2:8094756/1‑93 (MQ=255)

                                                                          ttCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGa               >  1:9159682/1‑93 (MQ=255)

                                                                          gtCGGTAGATAACTGGGACAGGCGTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGa               <  1:2729299/92‑1 (MQ=255)

                                                                           tCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGAt              <  2:3516644/93‑1 (MQ=255)

                                                                            cGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATc             >  2:7226744/1‑93 (MQ=255)

                                                                             gggAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCa            <  1:5985240/93‑1 (MQ=255)

                                                                                  aTAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAat       <  2:6224825/93‑1 (MQ=255)

                                                                                   tAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGGACCCTCGGCGATTTTGGCGACCCGATCATTAata      >  2:6244656/1‑93 (MQ=255)

                                                                                   tAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAata      <  1:4120920/93‑1 (MQ=255)

                                                                                   tAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAata      <  2:2932905/93‑1 (MQ=255)

                                                                                   tAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAata      <  2:733203/93‑1 (MQ=255)

                                                                                   tAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAata      <  1:6940181/93‑1 (MQ=255)

                                                                                   aaaCTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAata      <  1:5411290/92‑1 (MQ=255)

                                                                                    aaCTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAatat     <  2:4604058/93‑1 (MQ=255)

                                                                                     aCTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAtg    >  1:325921/1‑93 (MQ=255)

                                                                                     aCTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAtg    >  1:9824482/1‑93 (MQ=255)

CCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCT  >  NC_000913/1400298‑1400477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTGCA  >  NC_000913/1400295‑1400480

AAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGG                                                                                              <  SRR4302540.9311422/94‑1 (MQ=60)

  GCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGAC                                                                                            <  SRR4302540.4680138/94‑1 (MQ=60)

    CAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAG                                                                                          >  SRR4302540.518076/1‑94 (MQ=60)

    CAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAG                                                                                          <  SRR4302540.657232/94‑1 (MQ=60)

     AGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGG                                                                                         >  SRR4302540.1048844/1‑94 (MQ=60)

     AGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGG                                                                                         <  SRR4302540.5180480/94‑1 (MQ=60)

       TTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCT                                                                                       >  SRR4302540.8461681/1‑94 (MQ=60)

       TTCTTCGGAGTTCCATTCCGGGGGACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCT                                                                                       >  SRR4302540.3307633/1‑94 (MQ=60)

        TCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGTGATAACTGGGACAGGCTT                                                                                      >  SRR4302540.5410215/1‑94 (MQ=60)

         CTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTT                                                                                     >  SRR4302540.6878442/1‑94 (MQ=60)

          TTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTG                                                                                    <  SRR4302540.7256349/94‑1 (MQ=60)

          TTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTG                                                                                    <  SRR4302540.8006238/94‑1 (MQ=60)

           TCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGT                                                                                   <  SRR4302540.5557380/94‑1 (MQ=60)

            CGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTC                                                                                  <  SRR4302540.2186733/94‑1 (MQ=60)

            CGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTC                                                                                  <  SRR4302540.7477428/94‑1 (MQ=60)

            CGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTC                                                                                  <  SRR4302540.7973489/94‑1 (MQ=60)

             GGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCG                                                                                 >  SRR4302540.5305502/1‑94 (MQ=60)

              GAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGA                                                                                >  SRR4302540.9256660/1‑94 (MQ=60)

                GTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAG                                                                              <  SRR4302540.3304746/94‑1 (MQ=60)

                GTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAG                                                                              <  SRR4302540.7857827/94‑1 (MQ=60)

                GTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAG                                                                              >  SRR4302540.6180978/1‑94 (MQ=60)

                GTCCCATGCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAG                                                                              <  SRR4302540.9204614/94‑1 (MQ=60)

                 TTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGC                                                                             <  SRR4302540.2466153/94‑1 (MQ=60)

                 TTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGC                                                                             >  SRR4302540.4612431/1‑94 (MQ=60)

                 TTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGGGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGC                                                                             <  SRR4302540.339717/94‑1 (MQ=60)

                  TCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCG                                                                            <  SRR4302540.6420627/94‑1 (MQ=60)

                  TCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCG                                                                            <  SRR4302540.7099565/94‑1 (MQ=60)

                  TCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCG                                                                            <  SRR4302540.7099566/94‑1 (MQ=60)

                   CCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGG                                                                           >  SRR4302540.3479754/1‑94 (MQ=60)

                   CCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGG                                                                           >  SRR4302540.7416515/1‑94 (MQ=60)

                    CATTCCGGGGTACCAAAATGGGATAGCGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGC                                                                          <  SRR4302540.2902135/94‑1 (MQ=60)

                      TTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGAAT                                                                        >  SRR4302540.552034/1‑94 (MQ=60)

                         CGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCA                                                                     <  SRR4302540.7080182/94‑1 (MQ=60)

                          GGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAA                                                                    >  SRR4302540.4091890/1‑94 (MQ=60)

                          GGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAA                                                                    >  SRR4302540.3228863/1‑94 (MQ=60)

                            GGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACC                                                                  >  SRR4302540.5491409/1‑94 (MQ=60)

                            GGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACC                                                                  <  SRR4302540.8876812/94‑1 (MQ=60)

                            GGTACCAAAATGGGATAGGGCCTGGGACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACC                                                                  >  SRR4302540.5491410/1‑94 (MQ=60)

                               ACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTG                                                               <  SRR4302540.5093317/94‑1 (MQ=60)

                                 CAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGT                                                             <  SRR4302540.1737921/94‑1 (MQ=60)

                                 CAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGT                                                             <  SRR4302540.6342175/94‑1 (MQ=60)

                                 CAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGT                                                             >  SRR4302540.4085775/1‑94 (MQ=60)

                                  AAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTT                                                            <  SRR4302540.9133091/94‑1 (MQ=60)

                                   AAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTT                                                           <  SRR4302540.9959471/94‑1 (MQ=60)

                                   AAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTT                                                           <  SRR4302540.4241534/94‑1 (MQ=60)

                                    AATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTC                                                          >  SRR4302540.7241129/1‑94 (MQ=60)

                                    AATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTC                                                          >  SRR4302540.3684157/1‑94 (MQ=60)

                                    AATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTC                                                          >  SRR4302540.785853/1‑94 (MQ=60)

                                     ATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCG                                                         <  SRR4302540.1235503/94‑1 (MQ=60)

                                     ATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCG                                                         <  SRR4302540.7020642/94‑1 (MQ=60)

                                        GGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTC                                                      >  SRR4302540.3428545/1‑94 (MQ=60)

                                        GGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTC                                                      <  SRR4302540.505083/94‑1 (MQ=60)

                                         GATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCA                                                     >  SRR4302540.8045735/1‑94 (MQ=60)

                                            AGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCA                                                  <  SRR4302540.3398177/94‑1 (MQ=60)

                                             gtgtctgtgacgTGGCATGTTCTAAGGTGCGATTCGGGAGATACCTGGGACAGGCTTTGTCGAAGCGGCATGCAACCGTGGTTTCGGTCATCAA                                                 <  SRR4302540.587169/82‑1 (MQ=60)

                                              GGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAT                                                <  SRR4302540.914517/94‑1 (MQ=60)

                                              GGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAT                                                >  SRR4302540.2922213/1‑94 (MQ=60)

                                              GGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAT                                                <  SRR4302540.8338103/94‑1 (MQ=60)

                                              GGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAT                                                <  SRR4302540.6036094/94‑1 (MQ=60)

                                              GGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAT                                                >  SRR4302540.4913502/1‑94 (MQ=60)

                                              GGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAT                                                <  SRR4302540.7690871/94‑1 (MQ=60)

                                              GGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAT                                                <  SRR4302540.4429912/94‑1 (MQ=60)

                                              GGCCTGGGACATGGCATTTTCGAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAT                                                <  SRR4302540.511252/94‑1 (MQ=60)

                                                 CTGGGACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCC                                             <  SRR4302540.9401772/94‑1 (MQ=60)

                                                  TGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCG                                            <  SRR4302540.6787285/94‑1 (MQ=60)

                                                    GTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCC                                          >  SRR4302540.3331081/1‑94 (MQ=60)

                                                      ACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACC                                        <  SRR4302540.8649592/94‑1 (MQ=60)

                                                      ACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACC                                        >  SRR4302540.9919202/1‑94 (MQ=60)

                                                      ACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACC                                        >  SRR4302540.2013640/1‑94 (MQ=60)

                                                       CATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCT                                       >  SRR4302540.9978321/1‑94 (MQ=60)

                                                       CATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCT                                       >  SRR4302540.7477245/1‑94 (MQ=60)

                                                       CATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCT                                       >  SRR4302540.8204283/1‑94 (MQ=60)

                                                        ATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTT                                      >  SRR4302540.9111828/1‑94 (MQ=60)

                                                        ATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTT                                      >  SRR4302540.75707/1‑94 (MQ=60)

                                                         TGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTC                                     >  SRR4302540.9403463/1‑94 (MQ=60)

                                                         TGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTC                                     >  SRR4302540.5669084/1‑94 (MQ=60)

                                                         TGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACATTC                                     >  SRR4302540.9403462/1‑94 (MQ=60)

                                                          TGCATTTTCTAAGGTGCGATTCGGGAGATCACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCG                                    <  SRR4302540.8192497/94‑1 (MQ=60)

                                                          GGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCG                                    <  SRR4302540.7298843/94‑1 (MQ=60)

                                                           GCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGG                                   >  SRR4302540.6121105/1‑94 (MQ=60)

                                                             ATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCG                                 >  SRR4302540.8255846/1‑94 (MQ=60)

                                                             ATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCG                                 >  SRR4302540.9769093/1‑94 (MQ=60)

                                                             ATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCG                                 >  SRR4302540.6802598/1‑94 (MQ=60)

                                                             ATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCG                                 >  SRR4302540.139181/1‑94 (MQ=60)

                                                             ATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCG                                 >  SRR4302540.4585554/1‑94 (MQ=60)

                                                               TTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAT                               <  SRR4302540.6231688/94‑1 (MQ=60)

                                                               TTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAT                               <  SRR4302540.8557477/94‑1 (MQ=60)

                                                                 TCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCTGCACCTTCGGCGATTT                             >  SRR4302540.9096601/1‑94 (MQ=60)

                                                                 TCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTT                             >  SRR4302540.7939371/1‑94 (MQ=60)

                                                                  CTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTT                            >  SRR4302540.9115626/1‑94 (MQ=60)

                                                                   TAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTG                           <  SRR4302540.8705089/94‑1 (MQ=60)

                                                                   TAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTG                           <  SRR4302540.1232592/94‑1 (MQ=60)

                                                                    AAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGG                          >  SRR4302540.7308733/1‑94 (MQ=60)

                                                                     AGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGC                         <  SRR4302540.7737946/94‑1 (MQ=60)

                                                                     AGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGC                         >  SRR4302540.1288579/1‑94 (MQ=60)

                                                                      GGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCG                        >  SRR4302540.6927306/1‑94 (MQ=60)

                                                                        TGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGAC                      >  SRR4302540.1885814/1‑94 (MQ=60)

                                                                         GCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACC                     <  SRR4302540.5582512/94‑1 (MQ=60)

                                                                          CGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCC                    <  SRR4302540.5081341/94‑1 (MQ=60)

                                                                           GATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCG                   <  SRR4302540.1587646/94‑1 (MQ=60)

                                                                            ATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGA                  >  SRR4302540.9269622/1‑94 (MQ=60)

                                                                             TTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGAT                 <  SRR4302540.3311056/94‑1 (MQ=60)

                                                                             GTCGGTAGATAACTGGGACAGGCGTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGAT                 <  SRR4302540.2758742/94‑1 (MQ=60)

                                                                              TCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATC                <  SRR4302540.6926103/94‑1 (MQ=60)

                                                                                GGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCAT              <  SRR4302540.6051525/94‑1 (MQ=60)

                                                                                GGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCAT              >  SRR4302540.1250181/1‑94 (MQ=60)

                                                                                   AGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAA           >  SRR4302540.9937088/1‑94 (MQ=60)

                                                                                   AGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAA           <  SRR4302540.3133282/94‑1 (MQ=60)

                                                                                    GGTAACTGGCACAGGCTTTTTCGAAGCGGCATTCAACCGTTGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAAT          <  SRR4302540.8871876/94‑1 (MQ=60)

                                                                                      TAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAT        <  SRR4302540.5946339/94‑1 (MQ=60)

                                                                                      TAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAT        <  SRR4302540.54781/94‑1 (MQ=60)

                                                                                      TAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAT        <  SRR4302540.7017435/94‑1 (MQ=60)

                                                                                      TAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAT        <  SRR4302540.4165416/94‑1 (MQ=60)

                                                                                      TAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAT        <  SRR4302540.926880/94‑1 (MQ=60)

                                                                                      AAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAT        <  SRR4302540.5470785/94‑1 (MQ=60)

                                                                                       AACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATG       >  SRR4302540.329187/1‑94 (MQ=60)

                                                                                       AACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATG       >  SRR4302540.9939419/1‑94 (MQ=60)

                                                                                         CTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCT     <  SRR4302540.8310883/94‑1 (MQ=60)

                                                                                          TGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTG    <  SRR4302540.4988476/94‑1 (MQ=60)

                                                                                          TGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTG    >  SRR4302540.1290537/1‑94 (MQ=60)

                                                                                          TGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTG    >  SRR4302540.1290538/1‑94 (MQ=60)

                                                                                          TGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTG    >  SRR4302540.6841474/1‑94 (MQ=60)

                                                                                            GGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTGCA  <  SRR4302540.2545158/94‑1 (MQ=60)

                                                                                            GGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTGCA  <  SRR4302540.6189264/94‑1 (MQ=60)

                                                                                            GGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTGCA  <  SRR4302540.7541307/94‑1 (MQ=60)

AAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTGCA  >  NC_000913/1400295‑1400480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`