Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,400,388	A→G	S298P (TCC→CCC)	b1337 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,400,388	0	A	G	100.0%	566.9 / NA	174	S298P (TCC→CCC)	b1337	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (70/104); total (70/104)

Only 100 of 176 total aligned reads displayed.

CCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCT  >  NC_000913/1400298‑1400477

ccAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGAc                                                                                         <  1:3657393/93‑1 (MQ=255)

 cAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACa                                                                                        >  2:2968378/1‑93 (MQ=255)

 cAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACa                                                                                        <  1:5686196/93‑1 (MQ=255)

   gTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAgg                                                                                      >  1:2724205/1‑93 (MQ=255)

   gTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAgg                                                                                      >  2:5686502/1‑93 (MQ=255)

    ttcttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGc                                                                                     >  1:2636602/1‑93 (MQ=255)

      cttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCtt                                                                                   <  2:1913027/93‑1 (MQ=255)

      cttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCtt                                                                                   <  2:6289840/93‑1 (MQ=255)

       ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  <  2:627359/93‑1 (MQ=255)

       ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  >  2:1110291/1‑93 (MQ=255)

       ttcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCttt                                                                                  <  2:1721453/93‑1 (MQ=255)

        tcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTg                                                                                 >  2:2851460/1‑93 (MQ=255)

        tcGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTg                                                                                 >  2:445475/1‑93 (MQ=255)

         cGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGt                                                                                >  2:5459301/1‑93 (MQ=255)

          ggAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTc                                                                               >  2:3017749/1‑93 (MQ=255)

          ggAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTc                                                                               <  1:4908229/93‑1 (MQ=255)

          ggAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTc                                                                               >  1:2680986/1‑93 (MQ=255)

            aGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGa                                                                             <  1:2171755/93‑1 (MQ=255)

              ttCCATTCCGGGGACCCAAAATGGGATAGGGGCTGGTACATGGCATTTTCTAGGGTGCGATTCGGGAGATAGCTGGGACAGGCTTTGTCGAAg                                                                           >  2:2118092/1‑93 (MQ=255)

               tCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGc                                                                          >  2:3273280/1‑93 (MQ=255)

                 cATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCgg                                                                        <  1:4695575/93‑1 (MQ=255)

                   ttCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCa                                                                      <  1:4395418/93‑1 (MQ=255)

                      cGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTc                                                                   <  1:4243348/93‑1 (MQ=255)

                        gggTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCaa                                                                 >  2:1293908/1‑93 (MQ=255)

                        gggTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCaa                                                                 <  1:5757844/93‑1 (MQ=255)

                        gggTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCaa                                                                 <  2:4176811/93‑1 (MQ=255)

                          gTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAAcc                                                               <  2:3476773/93‑1 (MQ=255)

                            aCCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGt                                                             <  1:422933/93‑1 (MQ=255)

                            aCCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGt                                                             >  1:5794585/1‑93 (MQ=255)

                             ccAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTg                                                            <  1:3915488/93‑1 (MQ=255)

                              cAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTgg                                                           <  1:1124814/93‑1 (MQ=255)

                               aaaaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGt                                                          <  1:281672/93‑1 (MQ=255)

                                aaaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGtt                                                         <  1:5989127/93‑1 (MQ=255)

                                 aaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGttt                                                        >  2:625789/1‑93 (MQ=255)

                                 aaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGttt                                                        >  1:1621410/1‑93 (MQ=255)

                                 aaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGttt                                                        >  1:93106/1‑93 (MQ=255)

                                 aaTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGttt                                                        <  1:4931862/93‑1 (MQ=255)

                                  aTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTc                                                       <  1:1513206/93‑1 (MQ=255)

                                  aTGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTc                                                       >  1:456984/1‑93 (MQ=255)

                                    gggATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCgg                                                     >  2:5095536/1‑93 (MQ=255)

                                     ggATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGt                                                    <  1:4711023/93‑1 (MQ=255)

                                      gATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtc                                                   <  1:739517/93‑1 (MQ=255)

                                         aGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGtcatc                                                <  2:1704068/93‑1 (MQ=255)

                                            gCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAt                                             <  2:808287/93‑1 (MQ=255)

                                            gCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAt                                             <  2:1533662/93‑1 (MQ=255)

                                               tGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGcg                                          >  1:1732909/1‑92 (MQ=255)

                                               tGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGcc                                          >  2:1038296/1‑93 (MQ=255)

                                                ggTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCg                                         <  2:487104/93‑1 (MQ=255)

                                                ggTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCg                                         <  1:4089014/93‑1 (MQ=255)

                                                 gTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGc                                        >  1:2610413/1‑93 (MQ=255)

                                                    cATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCAcc                                     >  1:5474326/1‑93 (MQ=255)

                                                     aTGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCt                                    >  1:1352125/1‑93 (MQ=255)

                                                      tGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCtt                                   >  2:5480318/1‑93 (MQ=255)

                                                      tGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCtt                                   >  2:196797/1‑93 (MQ=255)

                                                       ggCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTc                                  <  1:3199320/93‑1 (MQ=255)

                                                        gCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCg                                 <  2:4566631/93‑1 (MQ=255)

                                                        gCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCg                                 <  1:2387786/93‑1 (MQ=255)

                                                        gCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCg                                 <  1:1015981/93‑1 (MQ=255)

                                                         cATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCgg                                <  2:3209353/93‑1 (MQ=255)

                                                          aTTTTCTAGGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGc                               >  1:2051212/1‑93 (MQ=255)

                                                          aTTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGc                               <  1:848075/93‑1 (MQ=255)

                                                            tttCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGa                             <  1:6093453/93‑1 (MQ=255)

                                                            tttCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGa                             <  2:5925270/93‑1 (MQ=255)

                                                            tttCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGa                             >  1:2867691/1‑93 (MQ=255)

                                                             ttCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAt                            <  1:2189191/93‑1 (MQ=255)

                                                              tCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAtt                           <  2:909917/93‑1 (MQ=255)

                                                              tCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAtt                           >  1:3734426/1‑93 (MQ=255)

                                                                tAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAtttt                         >  2:1312526/1‑93 (MQ=255)

                                                                tAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAtttt                         <  1:5646595/93‑1 (MQ=255)

                                                                tAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAtttt                         >  1:3403161/1‑93 (MQ=255)

                                                                  aGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTgg                       <  2:1411905/93‑1 (MQ=255)

                                                                  aGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTgg                       <  2:5546861/93‑1 (MQ=255)

                                                                  aGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTgg                       <  2:46878/93‑1 (MQ=255)

                                                                  aGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTgg                       <  1:4848309/93‑1 (MQ=255)

                                                                   ggagCGACTCGGGAAATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGATTCGGTCATCAATGCCGCACCTTCGGCGATTTTCGc                      >  2:2278330/4‑93 (MQ=255)

                                                                   ggTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGc                      <  2:2069666/93‑1 (MQ=255)

                                                                     tGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGa                    >  2:3508762/1‑93 (MQ=255)

                                                                        gATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGAccc                 <  1:2975234/93‑1 (MQ=255)

                                                                          ttCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCGATGCCGCACCTTCGGCGATTTTGGCGACCCGa               <  2:5840146/93‑1 (MQ=255)

                                                                          ttCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGa               <  1:1913280/93‑1 (MQ=255)

                                                                          ttCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGa               >  1:5588562/1‑93 (MQ=255)

                                                                          ttCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGa               >  1:2969210/1‑93 (MQ=255)

                                                                           tCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGAt              >  2:5257329/1‑93 (MQ=255)

                                                                            cGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATc             <  2:2675142/93‑1 (MQ=255)

                                                                            cGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATc             <  1:1769415/93‑1 (MQ=255)

                                                                            cGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATc             <  1:3372471/93‑1 (MQ=255)

                                                                            cGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATc             <  2:539965/93‑1 (MQ=255)

                                                                            cGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATc             <  1:2465739/93‑1 (MQ=255)

                                                                             gggAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCa            >  2:3645711/1‑93 (MQ=255)

                                                                              ggAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCAt           <  1:1641183/93‑1 (MQ=255)

                                                                               gagaTAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATa          <  2:4557537/93‑1 (MQ=255)

                                                                               gaaaTAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCCCCTTCGGCGATTTTGGCGACCCGATCATa          <  2:6305852/90‑1 (MQ=255)

                                                                                 gaTAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATaaa        >  2:5124655/1‑93 (MQ=255)

                                                                                 gaTAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATaaa        <  1:4946930/93‑1 (MQ=255)

                                                                                   tAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAata      <  2:4211808/93‑1 (MQ=255)

                                                                                     aCTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAtg    >  1:5562891/1‑93 (MQ=255)

                                                                                      cTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAtgc   >  1:5327928/1‑93 (MQ=255)

                                                                                      cTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAtgc   >  2:2865350/1‑93 (MQ=255)

                                                                                      cTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAtgc   <  2:4585483/93‑1 (MQ=255)

                                                                                       tGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAtgct  <  2:6181625/93‑1 (MQ=255)

CCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCT  >  NC_000913/1400298‑1400477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTG  >  NC_000913/1400295‑1400478

AAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGG                                                                                            <  SRR4302533.5726223/94‑1 (MQ=60)

   CCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACA                                                                                         <  SRR4302533.6250781/94‑1 (MQ=60)

   CCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACA                                                                                         <  SRR4302533.3679878/94‑1 (MQ=60)

    CAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAG                                                                                        <  SRR4302533.5720540/94‑1 (MQ=60)

     AGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGG                                                                                       >  SRR4302533.2740921/1‑94 (MQ=60)

      GTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGC                                                                                      >  SRR4302533.2652809/1‑94 (MQ=60)

      GTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGC                                                                                      <  SRR4302533.5140764/94‑1 (MQ=60)

       TTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCT                                                                                     >  SRR4302533.4085626/1‑94 (MQ=60)

          TTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTG                                                                                  <  SRR4302533.4947325/94‑1 (MQ=60)

          TTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTG                                                                                  <  SRR4302533.3534284/94‑1 (MQ=60)

            CGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTC                                                                                >  SRR4302533.2697413/1‑94 (MQ=60)

            CGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTC                                                                                >  SRR4302533.1601968/1‑94 (MQ=60)

             GGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCG                                                                               <  SRR4302533.4937967/94‑1 (MQ=60)

               AGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAA                                                                             <  SRR4302533.5776050/94‑1 (MQ=60)

               AGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAA                                                                             <  SRR4302533.2185109/94‑1 (MQ=60)

                GTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAG                                                                            >  SRR4302533.3338865/1‑94 (MQ=60)

                 TTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGC                                                                           <  SRR4302533.2393496/94‑1 (MQ=60)

                  TCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCG                                                                          <  SRR4302533.1729112/94‑1 (MQ=60)

                    CATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGC                                                                        <  SRR4302533.4724030/94‑1 (MQ=60)

                     ATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCA                                                                       >  SRR4302533.4540016/1‑94 (MQ=60)

                      TTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCAT                                                                      <  SRR4302533.4421956/94‑1 (MQ=60)

                       TCTGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATT                                                                     <  SRR4302533.74728/94‑1 (MQ=60)

                         CGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCA                                                                   <  SRR4302533.4268973/94‑1 (MQ=60)

                           GGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAAC                                                                 <  SRR4302533.5792589/94‑1 (MQ=60)

                            GGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACC                                                                <  SRR4302533.1875054/94‑1 (MQ=60)

                              TACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGT                                                              >  SRR4302533.5829592/1‑94 (MQ=60)

                               ACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTG                                                             <  SRR4302533.425464/94‑1 (MQ=60)

                                CCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGG                                                            <  SRR4302533.3939403/94‑1 (MQ=60)

                                 CAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGT                                                           <  SRR4302533.1131489/94‑1 (MQ=60)

                                  AAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTT                                                          <  SRR4302533.283395/94‑1 (MQ=60)

                                   AAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTT                                                         >  SRR4302533.1631236/1‑94 (MQ=60)

                                   AAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTT                                                         >  SRR4302533.93695/1‑94 (MQ=60)

                                   AAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTT                                                         <  SRR4302533.6025400/94‑1 (MQ=60)

                                    AATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTC                                                        >  SRR4302533.459716/1‑94 (MQ=60)

                                    AATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTC                                                        <  SRR4302533.4961728/94‑1 (MQ=60)

                                     ATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCG                                                       <  SRR4302533.1522322/94‑1 (MQ=60)

                                        GGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTC                                                    <  SRR4302533.4416666/94‑1 (MQ=60)

                                        GGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTC                                                    <  SRR4302533.481897/94‑1 (MQ=60)

                                        GGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTC                                                    <  SRR4302533.4739575/94‑1 (MQ=60)

                                         GATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCA                                                   >  SRR4302533.218873/1‑94 (MQ=60)

                                         GATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCA                                                   <  SRR4302533.743870/94‑1 (MQ=60)

                                              GGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAAT                                              <  SRR4302533.6074916/94‑1 (MQ=60)

                                                CCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGC                                            >  SRR4302533.2168669/1‑94 (MQ=60)

                                                 CTGGTACATTGCATTTTCTAAGGTACGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCC                                           <  SRR4302533.3214083/94‑1 (MQ=60)

                                                 CTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCG                                           >  SRR4302533.1743414/1‑94 (MQ=60)

                                                 CTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCC                                           >  SRR4302533.4235687/1‑94 (MQ=60)

                                                   GGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGC                                         >  SRR4302533.2626437/1‑94 (MQ=60)

                                                   GGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGC                                         <  SRR4302533.4113909/94‑1 (MQ=60)

                                                      ACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACC                                      >  SRR4302533.5507324/1‑94 (MQ=60)

                                                       CATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCT                                     >  SRR4302533.1360257/1‑94 (MQ=60)

                                                       CATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCT                                     >  SRR4302533.2644589/1‑94 (MQ=60)

                                                         TGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTC                                   <  SRR4302533.1400319/94‑1 (MQ=60)

                                                         TGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTC                                   <  SRR4302533.1268327/94‑1 (MQ=60)

                                                          GGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCG                                  <  SRR4302533.3219007/94‑1 (MQ=60)

                                                           GCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGG                                 <  SRR4302533.5988842/94‑1 (MQ=60)

                                                           GCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGG                                 <  SRR4302533.1021954/94‑1 (MQ=60)

                                                           GCATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGG                                 <  SRR4302533.2402537/94‑1 (MQ=60)

                                                            CATTTTCTAGGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGC                                >  SRR4302533.2063866/1‑94 (MQ=60)

                                                            CATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGC                                <  SRR4302533.5711458/94‑1 (MQ=60)

                                                             ATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCG                               <  SRR4302533.1770165/94‑1 (MQ=60)

                                                             ATTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCG                               <  SRR4302533.853038/94‑1 (MQ=60)

                                                              TTTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGA                              >  SRR4302533.2885334/1‑94 (MQ=60)

                                                               TTTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGAT                             <  SRR4302533.6130273/94‑1 (MQ=60)

                                                                TTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATT                            >  SRR4302533.3757331/1‑94 (MQ=60)

                                                                TTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATT                            >  SRR4302533.3394560/1‑94 (MQ=60)

                                                                TTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATT                            <  SRR4302533.2202679/94‑1 (MQ=60)

                                                                TTCTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAACCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATT                            <  SRR4302533.5652638/94‑1 (MQ=60)

                                                                  CTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTT                          <  SRR4302533.267959/94‑1 (MQ=60)

                                                                  CTAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTT                          >  SRR4302533.3424174/1‑94 (MQ=60)

                                                                   TAAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTG                         <  SRR4302533.5680729/94‑1 (MQ=60)

                                                                    AAGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGG                        <  SRR4302533.47237/94‑1 (MQ=60)

                                                                     AGGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGC                       <  SRR4302533.4877690/94‑1 (MQ=60)

                                                                      GGTGCGATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCG                      >  SRR4302533.981941/1‑94 (MQ=60)

                                                                           GATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCG                 <  SRR4302533.2993438/94‑1 (MQ=60)

                                                                           GATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCG                 >  SRR4302533.3885683/1‑94 (MQ=60)

                                                                            ATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGA                >  SRR4302533.5622326/1‑94 (MQ=60)

                                                                            ATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGA                >  SRR4302533.2987364/1‑94 (MQ=60)

                                                                            ATTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGA                >  SRR4302533.5448560/1‑94 (MQ=60)

                                                                             TTCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGAT               <  SRR4302533.1924955/94‑1 (MQ=60)

                                                                              TCGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATC              <  SRR4302533.5580492/94‑1 (MQ=60)

                                                                               CGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCA             <  SRR4302533.3393298/94‑1 (MQ=60)

                                                                               CGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCA             <  SRR4302533.1780187/94‑1 (MQ=60)

                                                                               CGGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCA             <  SRR4302533.2480949/94‑1 (MQ=60)

                                                                                GGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCAT            >  SRR4302533.2855036/1‑94 (MQ=60)

                                                                                GGGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCAT            <  SRR4302533.2581798/94‑1 (MQ=60)

                                                                                 GGAGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATA           <  SRR4302533.1651126/94‑1 (MQ=60)

                                                                                   AGATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAA         >  SRR4302533.4880291/1‑94 (MQ=60)

                                                                                    GATAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAAT        <  SRR4302533.4976874/94‑1 (MQ=60)

                                                                                      TAACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATAT      <  SRR4302533.2786267/94‑1 (MQ=60)

                                                                                       AACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATG     >  SRR4302533.5596514/1‑94 (MQ=60)

                                                                                        ACTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGC    >  SRR4302533.5359856/1‑94 (MQ=60)

                                                                                         CTGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCT   >  SRR4302533.2950804/1‑94 (MQ=60)

                                                                                          TGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTG  >  SRR4302533.2815137/1‑94 (MQ=60)

                                                                                          TGGGACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTG  >  SRR4302533.5658569/1‑94 (MQ=60)

AAGCCAGTTCTTCGGAGTTCCATTCCGGGGTACCAAAATGGGATAGGGCCTGGTACATGGCATTTTCTAAGGTGCGATTCGGGAGATAACTGGAACAGGCTTTGTCGAAGCGGCATTCAACCGTGGTTTCGGTCATCAATGCCGCACCTTCGGCGATTTTGGCGACCCGATCATAAATATGCTG  >  NC_000913/1400295‑1400478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`