Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,481,285 A→G A67A (GCA→GCG)  hrpA → helicase, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,481,2850AG99.7% 922.2 / ‑5.0 290A67A (GCA→GCG) hrpAhelicase, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (134/155);  total (135/155)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01

Only 100 of 300 total aligned reads displayed.

TAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAT  >  NC_000913/1481195‑1481374
                                                                                          |                                                                                         
tAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGcgcg                                                                                         >  1:6486537/1‑93 (MQ=255)
 aaaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGa                                                                                        <  2:1869851/93‑1 (MQ=255)
 aaaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGAGGCGCGa                                                                                        >  1:4028574/1‑93 (MQ=255)
  aaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGAc                                                                                       >  1:9691287/1‑93 (MQ=255)
  aaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGAc                                                                                       <  1:2404160/93‑1 (MQ=255)
  aaaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGAc                                                                                       <  1:2687375/93‑1 (MQ=255)
   aaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGAcc                                                                                      <  1:9033442/93‑1 (MQ=255)
   aaaTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGAcc                                                                                      >  1:7504276/1‑93 (MQ=255)
     aTCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCgg                                                                                    >  2:7805397/1‑93 (MQ=255)
      tCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGa                                                                                   >  2:2210714/1‑93 (MQ=255)
      tCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGa                                                                                   >  2:1140035/1‑93 (MQ=255)
       ccTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGaa                                                                                  >  1:220305/1‑93 (MQ=255)
        cTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGaaa                                                                                 <  2:964846/93‑1 (MQ=255)
        cTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGaaa                                                                                 <  2:7120360/93‑1 (MQ=255)
           aTGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTa                                                                              <  1:438856/93‑1 (MQ=255)
             gCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACt                                                                            >  1:5936526/1‑93 (MQ=255)
              cacaACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACtt                                                                           <  2:7674905/93‑1 (MQ=255)
               acaacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTa                                                                          >  1:5647836/1‑93 (MQ=255)
                caacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGGCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTAt                                                                         >  2:124613/1‑93 (MQ=255)
                 aacaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATc                                                                        <  2:2941057/93‑1 (MQ=255)
                  acaGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATcc                                                                       >  1:5821305/1‑93 (MQ=255)
                   caGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCt                                                                      <  1:697373/93‑1 (MQ=255)
                   caGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCt                                                                      <  1:5422789/93‑1 (MQ=255)
                   caGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCt                                                                      <  2:2702026/93‑1 (MQ=255)
                   caGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCt                                                                      <  1:1647682/93‑1 (MQ=255)
                   caGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCt                                                                      >  2:5438129/1‑93 (MQ=255)
                   caGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCt                                                                      >  1:2896375/1‑93 (MQ=255)
                    aGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTg                                                                     >  2:1468195/1‑93 (MQ=255)
                     ggCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGa                                                                    <  1:7164342/93‑1 (MQ=255)
                      gCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGAc                                                                   >  2:223299/1‑93 (MQ=255)
                      gCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGAc                                                                   <  2:6057283/93‑1 (MQ=255)
                      gCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGAc                                                                   <  2:4345294/93‑1 (MQ=255)
                          ttttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAAtt                                                               >  2:1782658/1‑93 (MQ=255)
                          ttttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAAtt                                                               >  1:6672071/1‑93 (MQ=255)
                          ttttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAAtt                                                               >  1:3473158/1‑93 (MQ=255)
                          ttttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAAtt                                                               <  2:8273012/93‑1 (MQ=255)
                           tttCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAAttt                                                              >  2:7616266/1‑93 (MQ=255)
                             tCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTAc                                                            <  2:8513700/93‑1 (MQ=255)
                               cAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCg                                                          <  2:4712571/93‑1 (MQ=255)
                               cAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCg                                                          >  2:3368238/1‑93 (MQ=255)
                               cAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCg                                                          >  1:957453/1‑93 (MQ=255)
                               cAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCg                                                          >  1:6711648/1‑93 (MQ=255)
                                aGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCgg                                                         >  1:4812686/1‑93 (MQ=255)
                                  gagaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGtt                                                       >  2:4882512/1‑93 (MQ=255)
                                  gagaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGtt                                                       <  2:5209616/93‑1 (MQ=255)
                                   agaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTa                                                      <  1:4848420/93‑1 (MQ=255)
                                    gaTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACCATTTACCGGTTAg                                                     >  2:1014956/1‑93 (MQ=255)
                                     agggCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGt                                                    <  1:8544624/91‑1 (MQ=255)
                                     aTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGt                                                    <  2:9946840/93‑1 (MQ=255)
                                     aTGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGt                                                    >  2:7888982/1‑93 (MQ=255)
                                      tGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGCCCGGCAATTACTTATCCTGCCAATTTACCGGTTAGTc                                                   >  2:1673559/1‑93 (MQ=255)
                                      tGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTc                                                   >  1:1772026/1‑93 (MQ=255)
                                       ggCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCa                                                  <  1:3322773/93‑1 (MQ=255)
                                        gCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAg                                                 >  1:2315932/1‑93 (MQ=255)
                                        gCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAg                                                 <  2:936348/93‑1 (MQ=255)
                                          gAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCTGaa                                               >  1:3947884/1‑93 (MQ=255)
                                          gAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGaa                                               >  1:4533666/1‑93 (MQ=255)
                                           aaaGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGaaa                                              >  2:6992155/1‑93 (MQ=255)
                                           aaaGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGaaa                                              >  1:473334/1‑93 (MQ=255)
                                            aaGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGaaaa                                             >  2:5832393/1‑93 (MQ=255)
                                              gagaTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGaaaaca                                           >  2:5613205/1‑91 (MQ=255)
                                                gaTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACa                                         >  2:9010426/1‑93 (MQ=255)
                                                gaTTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACa                                         >  2:4494052/1‑93 (MQ=255)
                                                  ttGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAgg                                       <  2:351120/93‑1 (MQ=255)
                                                    gACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGAc                                     >  2:636604/1‑93 (MQ=255)
                                                    gACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGAc                                     <  1:100618/93‑1 (MQ=255)
                                                    gACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGAc                                     >  2:7315428/1‑93 (MQ=255)
                                                     aCCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACa                                    <  2:2064528/93‑1 (MQ=255)
                                                      ccAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACAt                                   <  1:8377679/93‑1 (MQ=255)
                                                       cAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACAtt                                  >  2:4767720/1‑93 (MQ=255)
                                                       cAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACAtt                                  >  1:3415060/1‑93 (MQ=255)
                                                        aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATtc                                 <  2:2833353/93‑1 (MQ=255)
                                                        aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATtc                                 <  1:551767/93‑1 (MQ=255)
                                                        aGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATtc                                 <  2:1436467/93‑1 (MQ=255)
                                                          gcggcAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATtctc                               <  1:5238830/93‑1 (MQ=255)
                                                          gcggcAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATtctc                               <  2:6880775/93‑1 (MQ=255)
                                                            ggcAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGa                             <  2:6834089/93‑1 (MQ=255)
                                                             gcAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGaa                            <  2:4837304/93‑1 (MQ=255)
                                                               aGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGc                          >  1:7986295/1‑93 (MQ=255)
                                                                gggAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCg                         >  1:2770402/1‑93 (MQ=255)
                                                                  gAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGAt                       >  2:9119111/1‑93 (MQ=255)
                                                                  gAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCGGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGAt                       <  2:3817246/93‑1 (MQ=255)
                                                                     aGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCg                    >  2:6576790/1‑93 (MQ=255)
                                                                     aGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCg                    <  1:6262528/93‑1 (MQ=255)
                                                                     aGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCg                    <  2:9771128/93‑1 (MQ=255)
                                                                      gTCCTGCTGGGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGt                   <  1:1382618/93‑1 (MQ=255)
                                                                      gTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGt                   >  1:4890540/1‑93 (MQ=255)
                                                                        ccTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGa                 >  2:7535638/1‑93 (MQ=255)
                                                                        ccTGCTGCGTGAAGCGGCGCGACCGGAAATTACGTAGCCTGACAATTTACCGGTTAGTCAGAAAAGACAGGACATTCACGAAGCGATTCGTGa                 <  2:9833885/93‑1 (MQ=255)
                                                                         ctgctgCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGAt                >  2:8073581/1‑93 (MQ=255)
                                                                         ctgctgCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGAt                >  1:1547415/1‑93 (MQ=255)
                                                                          tgctgcGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATc               >  2:8149029/1‑93 (MQ=255)
                                                                            ctgcGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCAc             <  1:4235329/93‑1 (MQ=255)
                                                                             tgcGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCAcc            <  1:3740526/93‑1 (MQ=255)
                                                                              gcGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCa           <  2:5772999/93‑1 (MQ=255)
                                                                               cGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAg          <  2:2549535/93‑1 (MQ=255)
                                                                                    aGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAggtggt     <  2:1629356/93‑1 (MQ=255)
                                                                                     gcggcgCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGgtggtg    >  1:183680/1‑93 (MQ=255)
                                                                                       ggCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTTGTGATCACCAGGTGGTGAt  >  2:7528538/1‑93 (MQ=255)
                                                                                       ggCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAt  >  1:7341055/1‑93 (MQ=255)
                                                                                          |                                                                                         
TAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAT  >  NC_000913/1481195‑1481374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTC  >  NC_000913/1481192‑1481378
                                                                                             |                                                                                             
GGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCG                                                                                               <  SRR4302540.3865672/94‑1 (MQ=60)
 GTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGC                                                                                              >  SRR4302540.7878084/1‑94 (MQ=60)
  TTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCG                                                                                             >  SRR4302540.6558677/1‑94 (MQ=60)
   TAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGAGGCGCGA                                                                                            >  SRR4302540.4072017/1‑94 (MQ=60)
    AAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGAC                                                                                           >  SRR4302540.9805271/1‑94 (MQ=60)
     AAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACC                                                                                          <  SRR4302540.6184554/94‑1 (MQ=60)
     AAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACC                                                                                          >  SRR4302540.7588143/1‑94 (MQ=60)
     AAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACC                                                                                          <  SRR4302540.2430089/94‑1 (MQ=60)
     AAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACC                                                                                          <  SRR4302540.2716420/94‑1 (MQ=60)
     AAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACC                                                                                          <  SRR4302540.5625594/94‑1 (MQ=60)
     AAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACC                                                                                          <  SRR4302540.9796873/94‑1 (MQ=60)
     AAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACC                                                                                          <  SRR4302540.5563447/94‑1 (MQ=60)
      AAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCG                                                                                         <  SRR4302540.9141851/94‑1 (MQ=60)
      AAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCG                                                                                         <  SRR4302540.2795438/94‑1 (MQ=60)
         TCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAA                                                                                      >  SRR4302540.222445/1‑94 (MQ=60)
             GATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTA                                                                                  >  SRR4302540.1330759/1‑94 (MQ=60)
              ATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTAC                                                                                 <  SRR4302540.443518/94‑1 (MQ=60)
               TGCACAACAGGCCATTTTCCAGGAGATTGCGAAAGAGATTGACCCGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGcaacatact                                                                                >  SRR4302540.4403666/1‑85 (MQ=60)
               TGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACT                                                                                >  SRR4302540.6002150/1‑94 (MQ=60)
               TGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACT                                                                                >  SRR4302540.2485885/1‑94 (MQ=60)
                GCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCTTGAAGCGGCGCGACCGGAAATTACTT                                                                               >  SRR4302540.5603736/1‑94 (MQ=60)
                GCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTT                                                                               <  SRR4302540.3468782/94‑1 (MQ=60)
                 CACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTA                                                                              <  SRR4302540.6747884/94‑1 (MQ=60)
                 CACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTA                                                                              >  SRR4302540.5710002/1‑94 (MQ=60)
                  ACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTAT                                                                             <  SRR4302540.9800282/94‑1 (MQ=60)
                  ACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTAT                                                                             >  SRR4302540.3883346/1‑94 (MQ=60)
                  ACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTAT                                                                             <  SRR4302540.6902462/94‑1 (MQ=60)
                    AACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCC                                                                           >  SRR4302540.5885648/1‑94 (MQ=60)
                     ACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCT                                                                          <  SRR4302540.4668543/94‑1 (MQ=60)
                     ACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCT                                                                          >  SRR4302540.7279244/1‑94 (MQ=60)
                     ACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCT                                                                          >  SRR4302540.4822684/1‑94 (MQ=60)
                     ACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCT                                                                          >  SRR4302540.623358/1‑94 (MQ=60)
                     ACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCT                                                                          >  SRR4302540.2927959/1‑94 (MQ=60)
                      CAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTG                                                                         >  SRR4302540.1778921/1‑94 (MQ=60)
                      CAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTG                                                                         <  SRR4302540.5482422/94‑1 (MQ=60)
                      CAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTG                                                                         <  SRR4302540.1665663/94‑1 (MQ=60)
                      CAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTG                                                                         <  SRR4302540.704853/94‑1 (MQ=60)
                        GGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGAC                                                                       <  SRR4302540.8983488/94‑1 (MQ=60)
                        GGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGAC                                                                       <  SRR4302540.7244213/94‑1 (MQ=60)
                         GCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACA                                                                      <  SRR4302540.4980412/94‑1 (MQ=60)
                           CATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAAT                                                                    >  SRR4302540.2260366/1‑94 (MQ=60)
                            ATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATT                                                                   >  SRR4302540.4935127/1‑94 (MQ=60)
                            ATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATT                                                                   >  SRR4302540.4935126/1‑94 (MQ=60)
                            ATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATT                                                                   >  SRR4302540.4791175/1‑94 (MQ=60)
                            ATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATT                                                                   >  SRR4302540.3510971/1‑94 (MQ=60)
                            ATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATT                                                                   >  SRR4302540.6745790/1‑94 (MQ=60)
                            ATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATT                                                                   >  SRR4302540.2380984/1‑94 (MQ=60)
                             TTTTCCAGGAGATGGCGAAAGAGATCGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTT                                                                  <  SRR4302540.4722246/94‑1 (MQ=60)
                              TTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTA                                                                 >  SRR4302540.4875550/1‑94 (MQ=60)
                                TCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACC                                                               <  SRR4302540.1827438/94‑1 (MQ=60)
                                TCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACC                                                               <  SRR4302540.6457263/94‑1 (MQ=60)
                                TCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACC                                                               <  SRR4302540.1599413/94‑1 (MQ=60)
                                 CCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCG                                                              >  SRR4302540.5812659/1‑94 (MQ=60)
                                 CCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCG                                                              >  SRR4302540.967374/1‑94 (MQ=60)
                                 CCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCG                                                              >  SRR4302540.6785712/1‑94 (MQ=60)
                                  CAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGG                                                             >  SRR4302540.4864193/1‑94 (MQ=60)
                                  CAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGG                                                             >  SRR4302540.3971445/1‑94 (MQ=60)
                                   AGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGT                                                            <  SRR4302540.4020085/94‑1 (MQ=60)
                                    GGAGATGGCGAAAGAGATTGGCCAGGCGGCAGGGAAAGTCCTGCTGCGTGACGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTT                                                           <  SRR4302540.193228/94‑1 (MQ=60)
                                     GAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTA                                                          <  SRR4302540.2917840/94‑1 (MQ=60)
                                     GAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTA                                                          >  SRR4302540.5367873/1‑94 (MQ=60)
                                      AGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAG                                                         <  SRR4302540.2819111/94‑1 (MQ=60)
                                      AGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAG                                                         <  SRR4302540.4462743/94‑1 (MQ=60)
                                      AGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAG                                                         <  SRR4302540.4900363/94‑1 (MQ=60)
                                       GATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGT                                                        <  SRR4302540.9834183/94‑1 (MQ=60)
                                        ATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTC                                                       >  SRR4302540.1791257/1‑94 (MQ=60)
                                        ATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTC                                                       >  SRR4302540.6641129/1‑94 (MQ=60)
                                        ATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTC                                                       >  SRR4302540.676479/1‑94 (MQ=60)
                                        ATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTC                                                       >  SRR4302540.6641128/1‑94 (MQ=60)
                                        AGGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTC                                                       <  SRR4302540.8643901/94‑1 (MQ=60)
                                          GGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAG                                                     >  SRR4302540.2340862/1‑94 (MQ=60)
                                          GGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAG                                                     <  SRR4302540.3359226/94‑1 (MQ=60)
                                          GGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAG                                                     <  SRR4302540.5531780/94‑1 (MQ=60)
                                           GCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGA                                                    >  SRR4302540.7082471/1‑94 (MQ=60)
                                            CGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCTGAA                                                   >  SRR4302540.3990504/1‑94 (MQ=60)
                                            CGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAA                                                   >  SRR4302540.4582733/1‑94 (MQ=60)
                                            CGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAA                                                   >  SRR4302540.3059084/1‑94 (MQ=60)
                                            CGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAA                                                   <  SRR4302540.6493171/94‑1 (MQ=60)
                                             GAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAA                                                  >  SRR4302540.8273356/1‑94 (MQ=60)
                                             GAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAA                                                  >  SRR4302540.478401/1‑94 (MQ=60)
                                             GAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAA                                                  <  SRR4302540.3373473/94‑1 (MQ=60)
                                             GAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAA                                                  >  SRR4302540.3703022/1‑94 (MQ=60)
                                              AAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAA                                                 >  SRR4302540.9474870/1‑94 (MQ=60)
                                                AGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAA                                               <  SRR4302540.4409616/94‑1 (MQ=60)
                                                 AAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCGGACAATTTACCGGTTAGTCAGAAAAAAC                                              <  SRR4302540.3256308/94‑1 (MQ=60)
                                                  AGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACA                                             >  SRR4302540.5614044/1‑94 (MQ=60)
                                                    ATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCTGAAAAAACAGG                                           <  SRR4302540.862717/94‑1 (MQ=60)
                                                    ATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGG                                           <  SRR4302540.9877802/94‑1 (MQ=60)
                                                     TTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGA                                          <  SRR4302540.369702/94‑1 (MQ=60)
                                                       GACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACA                                        <  SRR4302540.101687/94‑1 (MQ=60)
                                                         CCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATT                                      <  SRR4302540.2288773/94‑1 (MQ=60)
                                                         CCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATT                                      <  SRR4302540.8474894/94‑1 (MQ=60)
                                                         CCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATT                                      >  SRR4302540.3452233/1‑94 (MQ=60)
                                                         CCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATT                                      >  SRR4302540.4068861/1‑94 (MQ=60)
                                                          CAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTC                                     >  SRR4302540.2344736/1‑94 (MQ=60)
                                                           AGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCT                                    <  SRR4302540.1760756/94‑1 (MQ=60)
                                                           AGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCT                                    <  SRR4302540.557785/94‑1 (MQ=60)
                                                            GGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTC                                   <  SRR4302540.4563310/94‑1 (MQ=60)
                                                             GCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCG                                  <  SRR4302540.5295981/94‑1 (MQ=60)
                                                               GGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGCA                                >  SRR4302540.3490811/1‑94 (MQ=60)
                                                               GGCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAA                                <  SRR4302540.8079119/94‑1 (MQ=60)
                                                                GCAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAG                               <  SRR4302540.5741079/94‑1 (MQ=60)
                                                                 CAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGC                              >  SRR4302540.437803/1‑94 (MQ=60)
                                                                 CAGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGC                              >  SRR4302540.8079292/1‑94 (MQ=60)
                                                                  AGGGAAAGTCCTGCTGCGTGAAGCGGCGCGCCCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAACAACAGGACCTTCTCGAAGCG                             >  SRR4302540.4898180/1‑94 (MQ=60)
                                                                  AGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG                             >  SRR4302540.2800340/1‑94 (MQ=60)
                                                                  AGGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCG                             <  SRR4302540.725744/94‑1 (MQ=60)
                                                                   GGGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGA                            >  SRR4302540.1586700/1‑94 (MQ=60)
                                                                    GGAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGAT                           <  SRR4302540.1888142/94‑1 (MQ=60)
                                                                     GAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATT                          <  SRR4302540.7061544/94‑1 (MQ=60)
                                                                     GAAAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATT                          <  SRR4302540.152092/94‑1 (MQ=60)
                                                                       AAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCG                        <  SRR4302540.6940082/94‑1 (MQ=60)
                                                                       AAGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCG                        <  SRR4302540.5164774/94‑1 (MQ=60)
                                                                        AGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGT                       >  SRR4302540.4943124/1‑94 (MQ=60)
                                                                        AGTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGT                       <  SRR4302540.6332204/94‑1 (MQ=60)
                                                                         GTCCTGCTGGGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTG                      <  SRR4302540.1397376/94‑1 (MQ=60)
                                                                         GTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTG                      >  SRR4302540.1811442/1‑94 (MQ=60)
                                                                         GTCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTG                      >  SRR4302540.5929278/1‑94 (MQ=60)
                                                                          TCCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGA                     <  SRR4302540.8343496/94‑1 (MQ=60)
                                                                           CCTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGAT                    >  SRR4302540.1564483/1‑94 (MQ=60)
                                                                            CTGCTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATC                   <  SRR4302540.8652729/94‑1 (MQ=60)
                                                                               CTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCCCC                <  SRR4302540.9560243/94‑1 (MQ=60)
                                                                               CTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACC                <  SRR4302540.2643428/94‑1 (MQ=60)
                                                                               CTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACC                <  SRR4302540.4281416/94‑1 (MQ=60)
                                                                               CTGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACC                >  SRR4302540.4157827/1‑94 (MQ=60)
                                                                               CTGCCGGAAGCGGCGCGACCGGAAATTACTTACCCTGACAATTTACCGGTTAGGCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACC                <  SRR4302540.2643426/94‑1 (MQ=60)
                                                                                TGCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCA               <  SRR4302540.3781333/94‑1 (MQ=60)
                                                                                 GCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAG              <  SRR4302540.7438384/94‑1 (MQ=60)
                                                                                 GCGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAG              <  SRR4302540.7438385/94‑1 (MQ=60)
                                                                                  CGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGG             <  SRR4302540.5343773/94‑1 (MQ=60)
                                                                                  CGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGG             <  SRR4302540.2767096/94‑1 (MQ=60)
                                                                                  CGTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGG             <  SRR4302540.3403803/94‑1 (MQ=60)
                                                                                   GTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGT            <  SRR4302540.5922288/94‑1 (MQ=60)
                                                                                   GTGAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGG            >  SRR4302540.1117449/1‑94 (MQ=60)
                                                                                     GAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGG          >  SRR4302540.8647724/1‑94 (MQ=60)
                                                                                     GAAGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGG          >  SRR4302540.1444830/1‑94 (MQ=60)
                                                                                       AGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTG        >  SRR4302540.185507/1‑94 (MQ=60)
                                                                                       AGCGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTG        >  SRR4302540.3268529/1‑94 (MQ=60)
                                                                                         CGGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGAT      >  SRR4302540.7422971/1‑94 (MQ=60)
                                                                                          GGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATT     <  SRR4302540.7713299/94‑1 (MQ=60)
                                                                                          GGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATT     <  SRR4302540.4135252/94‑1 (MQ=60)
                                                                                          GGCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATT     >  SRR4302540.7052216/1‑94 (MQ=60)
                                                                                           GCGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTG    >  SRR4302540.3386401/1‑94 (MQ=60)
                                                                                            CGCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGT   >  SRR4302540.9848338/1‑94 (MQ=60)
                                                                                             GCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTC  <  SRR4302540.5949867/94‑1 (MQ=60)
                                                                                             GCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTC  <  SRR4302540.1123767/94‑1 (MQ=60)
                                                                                             GCGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATTGTC  <  SRR4302540.1123766/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGTTAAAAATCCTGATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAAGTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGTCAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTC  >  NC_000913/1481192‑1481378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑