Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,482,154 T→C V357A (GTA→GCA)  hrpA → helicase, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,482,1540TC100.0% 659.4 / NA 209V357A (GTA→GCA) hrpAhelicase, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (88/121);  total (88/121)

Only 100 of 215 total aligned reads displayed.

TTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGA  >  NC_000913/1482064‑1482241
                                                                                          |                                                                                       
tttCGAACAGCGAACAAAATAGGGTATTCCAGTCTCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCAc                                                                                       <  1:6216039/93‑1 (MQ=255)
tttCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCAc                                                                                       <  1:1258672/93‑1 (MQ=255)
 ttCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCAcc                                                                                      >  1:8083916/1‑93 (MQ=255)
   cgcaCAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCACCGTCGCGGAAACGTCGCTGACCGCACCgg                                                                                    >  2:4388140/4‑93 (MQ=255)
   cGAACAGCGAACAAAATAGGGTATTCCAGTCGCTCAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCgg                                                                                    <  1:9692811/93‑1 (MQ=255)
   cGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCgg                                                                                    <  2:2899608/93‑1 (MQ=255)
    gAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCggg                                                                                   >  1:7643608/1‑93 (MQ=255)
    gAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCggg                                                                                   >  1:1842892/1‑93 (MQ=255)
     aaCAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCgggg                                                                                  >  1:1620110/1‑93 (MQ=255)
     aaCAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCgggg                                                                                  >  2:6597023/1‑93 (MQ=255)
     aaCAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCgggg                                                                                  >  1:8730796/1‑93 (MQ=255)
     aaCAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCgggg                                                                                  >  1:4149289/1‑93 (MQ=255)
      aCAGCTAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGa                                                                                 <  2:1445475/93‑1 (MQ=255)
       cAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGAt                                                                                <  2:6367263/93‑1 (MQ=255)
        tgCGAACAAAATAGGGTTTTCCAGTCGCACAGCGGACGGCGCATTGTGCTTGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGAtt                                                                               >  2:8976733/2‑93 (MQ=255)
         gCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTa                                                                              <  1:6930837/93‑1 (MQ=255)
           gAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTaaa                                                                            <  2:8466655/93‑1 (MQ=255)
           gAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTaaa                                                                            >  1:4491159/1‑93 (MQ=255)
           gAACAAAAGAGGGTGTTCCAGTCGCAGAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTaaa                                                                            <  2:5301257/93‑1 (MQ=255)
             aCAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATa                                                                          <  1:3900105/93‑1 (MQ=255)
             aCAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATa                                                                          <  2:2408839/93‑1 (MQ=255)
              cAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATAc                                                                         <  2:8239242/93‑1 (MQ=255)
              cAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATAc                                                                         <  2:5746751/93‑1 (MQ=255)
               aaaaTAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACg                                                                        <  1:294704/93‑1 (MQ=255)
                 aaTAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTAGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGtt                                                                      <  1:9437146/93‑1 (MQ=255)
                  aTAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAACCGTCGAGGACCGACCCGGGGATTAAAGACGtat                                                                     >  1:3351523/1‑91 (MQ=255)
                   tAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTAt                                                                    <  2:8600540/93‑1 (MQ=255)
                   tAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTAt                                                                    >  1:7983317/1‑93 (MQ=255)
                   tAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTAt                                                                    >  1:1713785/1‑93 (MQ=255)
                    aGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATc                                                                   <  2:4799346/93‑1 (MQ=255)
                     gggTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCg                                                                  <  2:3446959/93‑1 (MQ=255)
                       gTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAc                                                                <  1:1020822/93‑1 (MQ=255)
                        tATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAcc                                                               >  1:9146278/1‑93 (MQ=255)
                        tATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAcc                                                               >  2:3126845/1‑93 (MQ=255)
                        tATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAcc                                                               >  2:3182205/1‑93 (MQ=255)
                          ttCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAcccc                                                             >  1:9309580/1‑93 (MQ=255)
                             cAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGt                                                          <  2:1941018/93‑1 (MQ=255)
                             cAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGt                                                          <  2:1582292/93‑1 (MQ=255)
                             cAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGAACCGGGGATTAAATACGTTATCGACGCCGGt                                                          >  1:4484551/1‑93 (MQ=255)
                              aGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTa                                                         <  1:9064651/93‑1 (MQ=255)
                               gTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTAc                                                        <  1:2334924/93‑1 (MQ=255)
                               gTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTAc                                                        >  1:13126/1‑93 (MQ=255)
                                 cGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAg                                                      <  2:3951020/93‑1 (MQ=255)
                                 cGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAg                                                      <  1:10005610/93‑1 (MQ=255)
                                 cGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAg                                                      <  2:461633/93‑1 (MQ=255)
                                  gCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAgc                                                     <  1:5563898/93‑1 (MQ=255)
                                   cacaGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAgcg                                                    >  1:3285630/1‑93 (MQ=255)
                                       gCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTa                                                <  2:8385700/93‑1 (MQ=255)
                                        cGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGCATTAAATACGTTATCGACCCCGGTAAcgcgcggtg                                               >  1:3059679/1‑90 (MQ=255)
                                         ggACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATc                                              <  2:9378935/93‑1 (MQ=255)
                                          gACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCa                                             >  1:5155568/1‑93 (MQ=255)
                                           aCGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAg                                            >  2:9089847/1‑93 (MQ=255)
                                            cGGCGCATTGTGCTGGCGACCAACGTCGCGTAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGc                                           <  1:8650929/93‑1 (MQ=255)
                                            cGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGc                                           <  1:1995424/93‑1 (MQ=255)
                                            cGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGc                                           >  1:2723798/1‑93 (MQ=255)
                                            cGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGc                                           <  2:2138303/93‑1 (MQ=255)
                                             ggCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGcc                                          >  2:1441014/1‑93 (MQ=255)
                                                gcATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCt                                       >  2:2474445/1‑93 (MQ=255)
                                                 cATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTa                                      <  2:3622235/93‑1 (MQ=255)
                                                  aTTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTAc                                     >  1:8523881/1‑93 (MQ=255)
                                                  aTTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTAc                                     >  1:5285190/1‑93 (MQ=255)
                                                   ttGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTAcc                                    >  1:8365479/1‑92 (MQ=255)
                                                   ttGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACa                                    <  2:1611406/93‑1 (MQ=255)
                                                   ttGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACa                                    <  1:240006/93‑1 (MQ=255)
                                                     gtgCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGc                                  >  1:6497306/1‑93 (MQ=255)
                                                        cTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTAt                               <  1:5884214/93‑1 (MQ=255)
                                                         tGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATc                              <  2:2767130/93‑1 (MQ=255)
                                                         tGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATc                              <  1:9539623/93‑1 (MQ=255)
                                                         tGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATc                              <  2:8531306/93‑1 (MQ=255)
                                                            cGACCAGCGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCa                           <  2:3845521/93‑1 (MQ=255)
                                                            cGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACCGCTATCGCa                           >  2:6474623/1‑93 (MQ=255)
                                                            cGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCa                           <  1:5898676/93‑1 (MQ=255)
                                                             gACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCAc                          <  2:230458/93‑1 (MQ=255)
                                                              aCCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCAcc                         >  2:5458626/1‑93 (MQ=255)
                                                                 aaCGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCaaa                      >  1:382356/1‑93 (MQ=255)
                                                                 aaCGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCaaa                      <  2:7146519/93‑1 (MQ=255)
                                                                  aCGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAg                     <  1:6525707/93‑1 (MQ=255)
                                                                  aCGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAg                     >  2:5214813/1‑93 (MQ=255)
                                                                     tCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGc                  <  2:8107352/93‑1 (MQ=255)
                                                                       gcgGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAg                >  1:6403829/1‑93 (MQ=255)
                                                                        cgGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGc               <  2:1116414/93‑1 (MQ=255)
                                                                         ggAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCg              >  2:4205204/1‑93 (MQ=255)
                                                                         ggAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGGGAAGCg              >  1:2310479/1‑93 (MQ=255)
                                                                          gAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGt             >  2:6684947/1‑93 (MQ=255)
                                                                          gAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGt             >  1:609185/1‑93 (MQ=255)
                                                                           aaaCGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGtt            >  2:6527762/1‑93 (MQ=255)
                                                                           aaaCGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGtt            <  2:3219673/93‑1 (MQ=255)
                                                                           aaaCGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGtt            <  1:440868/93‑1 (MQ=255)
                                                                           aaaCGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGtt            <  1:8266451/93‑1 (MQ=255)
                                                                           aaaCGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCATATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGtt            <  2:7495410/93‑1 (MQ=255)
                                                                            aaCGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGttt           <  2:11630/93‑1 (MQ=255)
                                                                               gTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGcc        <  2:5076651/93‑1 (MQ=255)
                                                                                tCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCg       >  2:2265222/1‑93 (MQ=255)
                                                                                tCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCg       >  2:5976923/1‑93 (MQ=255)
                                                                                 cGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCATCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGa      <  1:6701834/93‑1 (MQ=255)
                                                                                 cGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGa      <  2:4076968/93‑1 (MQ=255)
                                                                                   cTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGAtt    >  2:7883399/1‑93 (MQ=255)
                                                                                   cTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGCGCAGCGTTTGCCGAtt    >  2:9737691/1‑93 (MQ=255)
                                                                                    tGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTg   <  1:5686186/93‑1 (MQ=255)
                                                                                     gACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGa  >  1:5439038/1‑93 (MQ=255)
                                                                                          |                                                                                       
TTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGA  >  NC_000913/1482064‑1482241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGAT  >  NC_000913/1482061‑1482247
                                                                                             |                                                                                             
GGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGC                                                                                               <  SRR4302540.4873466/94‑1 (MQ=60)
 GCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCA                                                                                              >  SRR4302540.1169680/1‑94 (MQ=60)
 GCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCA                                                                                              >  SRR4302540.9820656/1‑94 (MQ=60)
 GCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCCCATTGTGCTGGCGACCAACGTCGCGGACACGTCGCTGACCGCA                                                                                              >  SRR4302540.1169679/1‑94 (MQ=60)
  CTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCAC                                                                                             >  SRR4302540.572978/1‑94 (MQ=60)
  CTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCAC                                                                                             <  SRR4302540.5575136/94‑1 (MQ=60)
   TTTCGAACAGCGAACAAAATAGGGTATTCCAGTCTCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACC                                                                                            <  SRR4302540.6285200/94‑1 (MQ=60)
   TTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACC                                                                                            <  SRR4302540.2356686/94‑1 (MQ=60)
   TTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACC                                                                                            >  SRR4302540.8177738/1‑94 (MQ=60)
   TTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACC                                                                                            <  SRR4302540.1271578/94‑1 (MQ=60)
   TTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACC                                                                                            <  SRR4302540.1271577/94‑1 (MQ=60)
     TCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGG                                                                                          <  SRR4302540.1513590/94‑1 (MQ=60)
      CGAACAGCGAACAAAATAGGGTATTCCAGTCGCTCAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGG                                                                                         <  SRR4302540.9806812/94‑1 (MQ=60)
      CGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGG                                                                                         >  SRR4302540.7729636/1‑94 (MQ=60)
      CGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGG                                                                                         >  SRR4302540.1863081/1‑94 (MQ=60)
      CGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGG                                                                                         <  SRR4302540.1922518/94‑1 (MQ=60)
       GAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGG                                                                                        >  SRR4302540.8832182/1‑94 (MQ=60)
       GAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGG                                                                                        >  SRR4302540.1637873/1‑94 (MQ=60)
       GAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGG                                                                                        >  SRR4302540.4194214/1‑94 (MQ=60)
         ACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGAT                                                                                      >  SRR4302540.6609414/1‑94 (MQ=60)
          CAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATT                                                                                     >  SRR4302540.3868489/1‑94 (MQ=60)
          CAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATT                                                                                     <  SRR4302540.8020939/94‑1 (MQ=60)
            GCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAA                                                                                   <  SRR4302540.7007963/94‑1 (MQ=60)
             CGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAA                                                                                  >  SRR4302540.3227716/1‑94 (MQ=60)
             CGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAA                                                                                  >  SRR4302540.4539855/1‑94 (MQ=60)
             CGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAA                                                                                  <  SRR4302540.1706656/94‑1 (MQ=60)
                ACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCTACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATAC                                                                               <  SRR4302540.2214317/94‑1 (MQ=60)
                ACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATAC                                                                               <  SRR4302540.3942424/94‑1 (MQ=60)
                  AAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGT                                                                             <  SRR4302540.5226217/94‑1 (MQ=60)
                  AAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGT                                                                             <  SRR4302540.297611/94‑1 (MQ=60)
                  AAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGT                                                                             >  SRR4302540.839506/1‑94 (MQ=60)
                  AAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGT                                                                             <  SRR4302540.8106019/94‑1 (MQ=60)
                    AATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAACCGTCGAGGACCGACCCGGGGATTAAAgacgtat                                                                           >  SRR4302540.3388262/1‑87 (MQ=60)
                    AATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTA                                                                           >  SRR4302540.3388261/1‑94 (MQ=60)
                    AATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTA                                                                           >  SRR4302540.666527/1‑94 (MQ=60)
                    AATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTAGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTA                                                                           <  SRR4302540.9549224/94‑1 (MQ=60)
                     ATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTAT                                                                          >  SRR4302540.1732300/1‑94 (MQ=60)
                     ATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTAT                                                                          >  SRR4302540.8076289/1‑94 (MQ=60)
                      TAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATC                                                                         <  SRR4302540.2394046/94‑1 (MQ=60)
                        GGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGA                                                                       >  SRR4302540.6169355/1‑94 (MQ=60)
                         GGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGAC                                                                      <  SRR4302540.8608985/94‑1 (MQ=60)
                          GTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACC                                                                     >  SRR4302540.9256132/1‑94 (MQ=60)
                          GTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACC                                                                     <  SRR4302540.1031179/94‑1 (MQ=60)
                            ATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCC                                                                   >  SRR4302540.9420726/1‑94 (MQ=60)
                            ATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCC                                                                   >  SRR4302540.2980504/1‑94 (MQ=60)
                             TTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCG                                                                  <  SRR4302540.4116839/94‑1 (MQ=60)
                               CCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGT                                                                >  SRR4302540.4533191/1‑94 (MQ=60)
                               CCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGAACCGGGGATTAAATACGTTATCGACGCCGGT                                                                >  SRR4302540.4533190/1‑94 (MQ=60)
                                CAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTA                                                               <  SRR4302540.2614494/94‑1 (MQ=60)
                                 CGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTAC                                                              <  SRR4302540.9404199/94‑1 (MQ=60)
                                 AGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTAC                                                              >  SRR4302540.13269/1‑94 (MQ=60)
                                 AGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTAC                                                              <  SRR4302540.9173489/94‑1 (MQ=60)
                                  GTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACA                                                             >  SRR4302540.8823792/1‑94 (MQ=60)
                                  GTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACA                                                             <  SRR4302540.2360080/94‑1 (MQ=60)
                                    CGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGC                                                           <  SRR4302540.10122147/94‑1 (MQ=60)
                                     GCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCG                                                          <  SRR4302540.6337383/94‑1 (MQ=60)
                                     GCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCG                                                          <  SRR4302540.5625166/94‑1 (MQ=60)
                                     GCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCG                                                          >  SRR4302540.3321551/1‑94 (MQ=60)
                                          GCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGCATTAAATACGTTATCGACCCCGGTAacgcgcggtg                                                     >  SRR4302540.3093248/1‑84 (MQ=60)
                                            GGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCA                                                   >  SRR4302540.5211534/1‑94 (MQ=60)
                                            GGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCA                                                   <  SRR4302540.1112611/94‑1 (MQ=60)
                                            GGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCA                                                   >  SRR4302540.5755112/1‑94 (MQ=60)
                                              ACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGC                                                 >  SRR4302540.2753168/1‑94 (MQ=60)
                                               CGGCGCATTGTGCTGGCGACCAACGTCGCGTAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCC                                                <  SRR4302540.8751423/94‑1 (MQ=60)
                                               CGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCC                                                <  SRR4302540.9664852/94‑1 (MQ=60)
                                               CGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCC                                                <  SRR4302540.2017046/94‑1 (MQ=60)
                                                  CGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCT                                             <  SRR4302540.1761400/94‑1 (MQ=60)
                                                    CATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTAC                                           <  SRR4302540.9352149/94‑1 (MQ=60)
                                                    CATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTAC                                           >  SRR4302540.8622929/1‑94 (MQ=60)
                                                    CATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTAC                                           >  SRR4302540.1027266/1‑94 (MQ=60)
                                                    CATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTAC                                           >  SRR4302540.5342942/1‑94 (MQ=60)
                                                     ATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACC                                          >  SRR4302540.8462554/1‑94 (MQ=60)
                                                     ATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACA                                          >  SRR4302540.9501291/1‑94 (MQ=60)
                                                     ATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACA                                          >  SRR4302540.9488238/1‑94 (MQ=60)
                                                     ATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACA                                          <  SRR4302540.9185893/94‑1 (MQ=60)
                                                      TTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAG                                         <  SRR4302540.242329/94‑1 (MQ=60)
                                                      TTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAG                                         >  SRR4302540.5597957/1‑94 (MQ=60)
                                                       TGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGC                                        >  SRR4302540.6569552/1‑94 (MQ=60)
                                                          GCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTAT                                     >  SRR4302540.6006481/1‑94 (MQ=60)
                                                          GCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTAT                                     <  SRR4302540.9156158/94‑1 (MQ=60)
                                                          GCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTAT                                     <  SRR4302540.445862/94‑1 (MQ=60)
                                                           CTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCCCCCGGGATTAAATACGTTATAGACCCCCGGACAGCGGGTATCAGCCGCgccacctatc                                    >  SRR4302540.1196638/1‑84 (MQ=58)
                                                           CTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATC                                    <  SRR4302540.5949229/94‑1 (MQ=60)
                                                           CTGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGAATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATC                                    <  SRR4302540.5949230/94‑1 (MQ=60)
                                                            TGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCG                                   <  SRR4302540.9652517/94‑1 (MQ=60)
                                                            TGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCG                                   <  SRR4302540.9652516/94‑1 (MQ=60)
                                                            TGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCG                                   >  SRR4302540.4281737/1‑94 (MQ=60)
                                                            TGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCG                                   <  SRR4302540.6516369/94‑1 (MQ=60)
                                                            TGGCGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCG                                   <  SRR4302540.8457423/94‑1 (MQ=60)
                                                               CGACCAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCAC                                <  SRR4302540.5963821/94‑1 (MQ=60)
                                                                   CAACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAA                            >  SRR4302540.386309/1‑94 (MQ=60)
                                                                    AACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAG                           <  SRR4302540.6587680/94‑1 (MQ=60)
                                                                     ACGTCGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGT                          <  SRR4302540.6598181/94‑1 (MQ=60)
                                                                         CGCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAG                      >  SRR4302540.6475109/1‑94 (MQ=60)
                                                                          gaggcgACGTCGCTGACCGCACCGGGGTTTAAATACGTTATCAACCCCGGTACAGCGCGTAACAAAAAATACAGCTATCGCACCAAAGTGCAGC                     <  SRR4302540.9043019/88‑1 (MQ=42)
                                                                          GCGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGC                     <  SRR4302540.4463125/94‑1 (MQ=60)
                                                                           CGGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGGGAAGCG                    >  SRR4302540.2335351/1‑94 (MQ=60)
                                                                            GGAAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGT                   >  SRR4302540.615697/1‑94 (MQ=60)
                                                                              AAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCACAGTGCAGCGTTT                 >  SRR4302540.8286335/1‑94 (MQ=60)
                                                                              AAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTT                 <  SRR4302540.445562/94‑1 (MQ=60)
                                                                              AAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTT                 <  SRR4302540.3196850/94‑1 (MQ=60)
                                                                              AAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTT                 <  SRR4302540.8649386/94‑1 (MQ=60)
                                                                              AAACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTT                 <  SRR4302540.8362502/94‑1 (MQ=60)
                                                                               AACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTG                >  SRR4302540.75714/1‑94 (MQ=60)
                                                                                ACGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGC               <  SRR4302540.4620094/94‑1 (MQ=60)
                                                                                 CGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCC              >  SRR4302540.276000/1‑94 (MQ=60)
                                                                                 CGTCGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCC              >  SRR4302540.58953/1‑94 (MQ=60)
                                                                                    CGCTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCATCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGAT           <  SRR4302540.6775810/94‑1 (MQ=60)
                                                                                      CTGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTG         >  SRR4302540.1920535/1‑94 (MQ=60)
                                                                                       TGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGA        >  SRR4302540.5447115/1‑94 (MQ=60)
                                                                                       TGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGA        >  SRR4302540.5498875/1‑94 (MQ=60)
                                                                                       TGACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGA        <  SRR4302540.5748780/94‑1 (MQ=60)
                                                                                        GACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAG       <  SRR4302540.3628416/94‑1 (MQ=60)
                                                                                        GACCGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAG       <  SRR4302540.6798380/94‑1 (MQ=60)
                                                                                           CGCACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCG    <  SRR4302540.7865541/94‑1 (MQ=60)
                                                                                             CACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGAT  >  SRR4302540.1555534/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGAT  >  NC_000913/1482061‑1482247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑