Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,483,908 A→G S942G (AGC→GGC)  hrpA → helicase, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,483,9080AG88.5% 437.9 / 42.2 183S942G (AGC→GGC) hrpAhelicase, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (12/9);  new base G (87/75);  total (99/84)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 187 total aligned reads displayed.

AAACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAATT  >  NC_000913/1483818‑1483997
                                                                                          |                                                                                         
aaaCCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGc                                                                                         >  1:6474251/1‑93 (MQ=255)
aaaCCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGc                                                                                         >  1:6474250/1‑93 (MQ=255)
aaaCCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGc                                                                                         <  2:7622119/93‑1 (MQ=255)
aaaCCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCCTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGc                                                                                         >  2:5870950/1‑93 (MQ=255)
 aaCCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGcc                                                                                        <  1:6617406/93‑1 (MQ=255)
  aCCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCt                                                                                       <  2:2777233/93‑1 (MQ=255)
   ccGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCtt                                                                                      <  2:3535779/93‑1 (MQ=255)
   ccGGTACGCCGTAATTTTGTACCCGCGCCAAAATATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCtt                                                                                      <  1:9957526/93‑1 (MQ=255)
     ggTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGa                                                                                    >  2:8709115/1‑93 (MQ=255)
       tACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAgc                                                                                  <  1:598970/93‑1 (MQ=255)
       tACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAgc                                                                                  >  1:3823848/1‑93 (MQ=255)
       tACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAgc                                                                                  >  1:4988156/1‑93 (MQ=255)
        aCGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGCCGGCCTTGAgcg                                                                                 >  2:3531001/1‑93 (MQ=255)
           ccGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGa                                                                              <  2:7711643/93‑1 (MQ=255)
              tAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGtt                                                                           <  2:8028924/93‑1 (MQ=255)
                  tttGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTAcgg                                                                       <  2:2894356/93‑1 (MQ=255)
                   ttGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTAcggg                                                                      >  1:9276585/1‑92 (MQ=255)
                   ttGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTAcggc                                                                      <  2:1896222/93‑1 (MQ=255)
                   ttGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTAcggc                                                                      <  2:4196694/93‑1 (MQ=255)
                    tGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTAcggcg                                                                     <  2:7035743/93‑1 (MQ=255)
                      tACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGa                                                                   <  2:5814624/93‑1 (MQ=255)
                      tACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGa                                                                   >  1:1236136/1‑93 (MQ=255)
                      tACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGa                                                                   >  2:9593312/1‑93 (MQ=255)
                         ccGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGa                                                                <  1:2272527/93‑1 (MQ=255)
                          cgcgCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGAc                                                               >  1:6294639/1‑93 (MQ=255)
                          cgcgCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGAc                                                               <  1:7291523/93‑1 (MQ=255)
                          cgcgCCAAAATATGCCGAAGCGTTTTTAGGCCGCCTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGAc                                                               <  1:7767420/93‑1 (MQ=255)
                           gcgcCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGAcc                                                              <  2:50910/93‑1 (MQ=255)
                               cAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCg                                                          >  2:9468833/1‑93 (MQ=255)
                               cAAACTATGCCGAAGCGTTGTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCg                                                          <  2:4781603/93‑1 (MQ=255)
                                  aCTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTa                                                       >  2:5349924/1‑93 (MQ=255)
                                   cTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTAc                                                      <  1:91915/93‑1 (MQ=255)
                                   cTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTAc                                                      <  2:8452438/93‑1 (MQ=255)
                                    tATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTAcc                                                     >  2:1970286/1‑93 (MQ=255)
                                      tGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGt                                                   >  1:3797493/1‑93 (MQ=255)
                                       gCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGtt                                                  <  2:4148401/93‑1 (MQ=255)
                                        ccGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTg                                                 <  1:8698768/93‑1 (MQ=255)
                                        ccGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTg                                                 >  2:4358891/1‑93 (MQ=255)
                                         cGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGa                                                <  1:2172884/93‑1 (MQ=255)
                                         cGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGa                                                >  1:9091108/1‑93 (MQ=255)
                                          gAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGAc                                               <  1:7027234/93‑1 (MQ=255)
                                          gAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGAc                                               >  1:1843105/1‑93 (MQ=255)
                                          gAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGAc                                               >  1:8482468/1‑93 (MQ=255)
                                           aaGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGAcc                                              >  1:298724/1‑93 (MQ=255)
                                           aaGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGAcc                                              <  1:6546631/93‑1 (MQ=255)
                                            aGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACcg                                             >  1:5547371/1‑93 (MQ=255)
                                             gCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACcgc                                            >  1:3244984/1‑93 (MQ=255)
                                              cGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACcgcg                                           >  1:1023819/1‑93 (MQ=255)
                                              cGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGGTACCGTGGAcgggg                                           >  1:1023820/1‑89 (MQ=255)
                                               gTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGa                                          >  1:4273671/1‑93 (MQ=255)
                                                tttttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGaa                                         <  2:7046751/93‑1 (MQ=255)
                                                tttttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGaa                                         >  2:6412194/1‑93 (MQ=255)
                                                tttttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGaa                                         >  1:8298335/1‑93 (MQ=255)
                                                 ttttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGAACGGCGTTACCGTTGACCGCGAAg                                        >  1:7201200/1‑93 (MQ=255)
                                                  tttAGGCCGCGTCAAACCGCTGTAGTTACCGTTGCTCCGTGACCTTGAGCGCGAGTTACGGCGGATGACAGGCGGTACCGTTGGCCGCGaaat                                       >  2:2658351/1‑91 (MQ=255)
                                                  tttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGa                                       <  2:1540748/93‑1 (MQ=255)
                                                  tttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGa                                       <  1:9849991/93‑1 (MQ=255)
                                                  tttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGa                                       <  2:1761344/93‑1 (MQ=255)
                                                   ttAGTCCCCGTAAAACCGCTGGAGTTACCGTTGCTCTACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTCCCGTTGACCGCGAAGAc                                      <  2:5126851/93‑1 (MQ=255)
                                                   ttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGAc                                      <  2:9965087/93‑1 (MQ=255)
                                                   ttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGAc                                      <  2:9121417/93‑1 (MQ=255)
                                                    tAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACt                                     >  1:4371881/1‑93 (MQ=255)
                                                     aGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTg                                    <  1:2082849/93‑1 (MQ=255)
                                                     aGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTg                                    <  1:8136610/93‑1 (MQ=255)
                                                     aGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTg                                    <  2:2120197/93‑1 (MQ=255)
                                                      ggCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTgg                                   >  2:1042164/1‑93 (MQ=255)
                                                       gCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGc                                  <  2:376443/93‑1 (MQ=255)
                                                        ccGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCa                                 <  1:2554804/93‑1 (MQ=255)
                                                         cgcgTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCAc                                >  1:7096543/1‑93 (MQ=255)
                                                                agccGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATc                         <  2:7067578/91‑1 (MQ=255)
                                                                aaCCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATc                         >  1:7440479/1‑93 (MQ=255)
                                                                aaCCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCGCTGGCATc                         >  1:5276838/1‑93 (MQ=255)
                                                                 aCCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCa                        <  2:1531515/93‑1 (MQ=255)
                                                                  ccGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAg                       >  1:9625061/1‑93 (MQ=255)
                                                                  ccGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAg                       <  2:6710184/93‑1 (MQ=255)
                                                                   cGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAgg                      >  2:411447/1‑93 (MQ=255)
                                                                    gCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGt                     >  1:4042752/1‑93 (MQ=255)
                                                                     cTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTg                    >  2:7275575/1‑93 (MQ=255)
                                                                       ggAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGcc                  >  2:2695368/1‑93 (MQ=255)
                                                                       ggAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGcc                  <  1:3621580/93‑1 (MQ=255)
                                                                        gAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGccc                 <  2:2632969/93‑1 (MQ=255)
                                                                        gAGTTACCGTTGCTCGACGGCATTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGccc                 <  1:1119065/93‑1 (MQ=255)
                                                                        gAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGccc                 <  1:2204365/93‑1 (MQ=255)
                                                                          gTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGa               >  2:8539090/1‑93 (MQ=255)
                                                                          gTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGa               >  1:358097/1‑93 (MQ=255)
                                                                           ttACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGAt              <  2:2349270/93‑1 (MQ=255)
                                                                            tACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCAATGGGATCAGGTGCCCGATc             <  2:4069833/93‑1 (MQ=255)
                                                                             aCCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCa            >  2:6133787/1‑93 (MQ=255)
                                                                               cGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCAcc          >  2:8364096/1‑93 (MQ=255)
                                                                               cGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCAcc          >  1:1625990/1‑93 (MQ=255)
                                                                                gTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCt         >  2:6606462/1‑93 (MQ=255)
                                                                                gTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCt         >  2:1533888/1‑93 (MQ=255)
                                                                                 ttGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGTCCCATCACCTg        >  1:1431907/1‑93 (MQ=255)
                                                                                 ttGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTg        >  1:6719765/1‑93 (MQ=255)
                                                                                  tGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGa       >  1:4759497/1‑93 (MQ=255)
                                                                                   gCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGaa      >  1:2699720/1‑93 (MQ=255)
                                                                                     tCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGaaaa    <  1:4071373/93‑1 (MQ=255)
                                                                                      cGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAAt   >  1:6021241/1‑93 (MQ=255)
                                                                                       gACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAAtt  >  2:2445963/1‑93 (MQ=255)
                                                                                       gACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAAtt  <  2:9281505/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAATT  >  NC_000913/1483818‑1483997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGAAACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAATTACCT  >  NC_000913/1483815‑1484001
                                                                                             |                                                                                             
CCGAAACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACG                                                                                               <  SRR4302540.6036136/94‑1 (MQ=60)
 CGAAACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGG                                                                                              >  SRR4302540.4616044/1‑94 (MQ=60)
 CGAAACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGG                                                                                              <  SRR4302540.6593310/94‑1 (MQ=60)
  GAAACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGC                                                                                             >  SRR4302540.9831606/1‑94 (MQ=60)
  GAAACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGC                                                                                             >  SRR4302540.6546287/1‑94 (MQ=60)
  GAAACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGC                                                                                             >  SRR4302540.6546288/1‑94 (MQ=60)
    AACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCT                                                                                           <  SRR4302540.6690689/94‑1 (MQ=60)
      CCGGTACGCCGTAATTTTGTACCCGCGCCAAAATATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTG                                                                                         <  SRR4302540.10073740/94‑1 (MQ=60)
        GGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAG                                                                                       <  SRR4302540.5342509/94‑1 (MQ=60)
        GGGACGCCGTAATTTTGTCCCCGCGCCAAACTGTCCCGAAGCGTTTTTAGGCCGCGTCAACCCGCTGGAGTTACCGTTGCTCGACGGCCTTGAG                                                                                       <  SRR4302540.4583742/94‑1 (MQ=60)
         GTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGC                                                                                      >  SRR4302540.3865456/1‑94 (MQ=60)
         GTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGC                                                                                      <  SRR4302540.7800926/94‑1 (MQ=60)
         GTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGC                                                                                      >  SRR4302540.5042164/1‑94 (MQ=60)
          TACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCG                                                                                     >  SRR4302540.9073825/1‑94 (MQ=60)
          TACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCG                                                                                     <  SRR4302540.605395/94‑1 (MQ=60)
              CCGTAATTTTGTCCCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAG                                                                                 <  SRR4302540.2279497/94‑1 (MQ=60)
               CGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGT                                                                                >  SRR4302540.40919/1‑94 (MQ=60)
                     TTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGG                                                                          >  SRR4302540.9387488/1‑94 (MQ=60)
                       TGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGG                                                                        >  SRR4302540.6682060/1‑94 (MQ=60)
                        GTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGA                                                                       >  SRR4302540.1248758/1‑94 (MQ=60)
                          ACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATG                                                                     <  SRR4302540.1821299/94‑1 (MQ=60)
                           CCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGA                                                                    >  SRR4302540.995702/1‑94 (MQ=60)
                            CCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGAC                                                                   >  SRR4302540.6364723/1‑94 (MQ=60)
                            CCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGAC                                                                   >  SRR4302540.6364722/1‑94 (MQ=60)
                            CCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGAC                                                                   <  SRR4302540.2296920/94‑1 (MQ=60)
                             CGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACC                                                                  <  SRR4302540.7372892/94‑1 (MQ=60)
                             CGCGCCAAAATATGCCGAAGCGTTTTTAGGCCGCCTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACC                                                                  <  SRR4302540.7858228/94‑1 (MQ=60)
                                GCCAAACTATGCCGAAGCGTTTTTAGTCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGC                                                               <  SRR4302540.6169303/94‑1 (MQ=60)
                                GCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGC                                                               <  SRR4302540.9716427/94‑1 (MQ=60)
                                 CCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCG                                                              >  SRR4302540.1246055/1‑94 (MQ=60)
                                  CAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGT                                                             >  SRR4302540.8487965/1‑94 (MQ=60)
                                      CTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACC                                                         <  SRR4302540.92883/94‑1 (MQ=60)
                                        ATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGT                                                       >  SRR4302540.3838874/1‑94 (MQ=60)
                                         TGCCGAAGCGTTTTTAGGCTGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCCTGAGCGCGAGgtaaggcggcttactggcgtgtccgtt                                                      >  SRR4302540.7442689/1‑67 (MQ=60)
                                           CCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGA                                                    <  SRR4302540.8799745/94‑1 (MQ=60)
                                           CCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGA                                                    >  SRR4302540.9200329/1‑94 (MQ=60)
                                            CGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGAC                                                   >  SRR4302540.8581005/1‑94 (MQ=60)
                                            CGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGAC                                                   <  SRR4302540.2196318/94‑1 (MQ=60)
                                            CGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGAC                                                   >  SRR4302540.1863297/1‑94 (MQ=60)
                                            CGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTGGAC                                                   >  SRR4302540.8265299/1‑94 (MQ=60)
                                             GAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACC                                                  <  SRR4302540.7105308/94‑1 (MQ=60)
                                             GAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACC                                                  >  SRR4302540.301678/1‑94 (MQ=60)
                                              AAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCG                                                 >  SRR4302540.5608484/1‑94 (MQ=60)
                                              AAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCG                                                 <  SRR4302540.6619259/94‑1 (MQ=60)
                                               AGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGC                                                >  SRR4302540.5054421/1‑94 (MQ=60)
                                               AGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGC                                                >  SRR4302540.3280311/1‑94 (MQ=60)
                                                GCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCG                                               >  SRR4302540.1034206/1‑94 (MQ=60)
                                                GCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGGTACCGTggacgggg                                               >  SRR4302540.1034207/1‑86 (MQ=60)
                                                 CGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGA                                              >  SRR4302540.4320149/1‑94 (MQ=60)
                                                 CGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGA                                              >  SRR4302540.4320148/1‑94 (MQ=60)
                                                  GTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAA                                             >  SRR4302540.4143901/1‑94 (MQ=60)
                                                  GTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAA                                             >  SRR4302540.7250665/1‑94 (MQ=60)
                                                  GTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAA                                             >  SRR4302540.3148178/1‑94 (MQ=60)
                                                  GTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAA                                             >  SRR4302540.8394688/1‑94 (MQ=60)
                                                   TTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGAACGGCGTTACCGTTGACCGCGAAG                                            >  SRR4302540.7281485/1‑94 (MQ=60)
                                                     TTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGAC                                          <  SRR4302540.7175346/94‑1 (MQ=60)
                                                     TTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGAC                                          <  SRR4302540.9965124/94‑1 (MQ=60)
                                                     TTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTCGACCGCGAAGAC                                          >  SRR4302540.4070880/1‑94 (MQ=60)
                                                     TTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGAC                                          <  SRR4302540.6018096/94‑1 (MQ=60)
                                                      TTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACT                                         >  SRR4302540.4419372/1‑94 (MQ=60)
                                                      TTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACT                                         >  SRR4302540.7307626/1‑94 (MQ=60)
                                                       TAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTG                                        <  SRR4302540.1540041/94‑1 (MQ=60)
                                                        AGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGG                                       <  SRR4302540.2105222/94‑1 (MQ=60)
                                                        AGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGG                                       <  SRR4302540.8231044/94‑1 (MQ=60)
                                                          GCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCA                                     <  SRR4302540.4721292/94‑1 (MQ=60)
                                                           CCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCAC                                    <  SRR4302540.2582328/94‑1 (MQ=60)
                                                           CCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGAAC                                    >  SRR4302540.2805152/1‑94 (MQ=60)
                                                           CCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCAGCGTTACCGTTGACCGCGAAGACTGGCAC                                    <  SRR4302540.3863592/94‑1 (MQ=60)
                                                           CCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCAC                                    >  SRR4302540.7175516/1‑94 (MQ=60)
                                                            CGCGTCAAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACT                                   <  SRR4302540.654693/94‑1 (MQ=60)
                                                                  AAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATC                             >  SRR4302540.8637073/1‑94 (MQ=60)
                                                                  AAACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATC                             >  SRR4302540.7523382/1‑94 (MQ=60)
                                                                  AAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCGCTGGCATC                             >  SRR4302540.5334455/1‑94 (MQ=60)
                                                                   AACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCA                            <  SRR4302540.2568263/94‑1 (MQ=60)
                                                                    ACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAG                           >  SRR4302540.2617976/1‑94 (MQ=60)
                                                                    ACCGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAG                           >  SRR4302540.9738496/1‑94 (MQ=60)
                                                                      CGCTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGT                         >  SRR4302540.4086332/1‑94 (MQ=60)
                                                                       GCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGCTCAGGTG                        >  SRR4302540.2207326/1‑94 (MQ=60)
                                                                        CTGGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCACGAAGACTGGCACTGGGATCAGGAGC                       >  SRR4302540.3439502/1‑94 (MQ=60)
                                                                          GGAGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCC                     <  SRR4302540.3661202/94‑1 (MQ=60)
                                                                           GAGTTACCGTTGCTCGACGGCATTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCG                    <  SRR4302540.1130672/94‑1 (MQ=60)
                                                                           GAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCG                    <  SRR4302540.2228180/94‑1 (MQ=60)
                                                                            AGTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGA                   >  SRR4302540.361781/1‑94 (MQ=60)
                                                                             GTTACCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGAT                  >  SRR4302540.9228235/1‑94 (MQ=60)
                                                                                tcccTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCAC               <  SRR4302540.1337947/90‑1 (MQ=60)
                                                                                 CCGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACC              >  SRR4302540.1643798/1‑94 (MQ=60)
                                                                                  CGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCT             <  SRR4302540.6087625/94‑1 (MQ=60)
                                                                                  CGTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCT             >  SRR4302540.36884/1‑94 (MQ=60)
                                                                                  CGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGAACAGGTGCCCGATCACCT             >  SRR4302540.7059862/1‑94 (MQ=60)
                                                                                   GTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGTCCCATCACCTG            >  SRR4302540.1447423/1‑94 (MQ=60)
                                                                                   GTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTG            >  SRR4302540.6793921/1‑94 (MQ=60)
                                                                                   GTTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTA            >  SRR4302540.6793920/1‑94 (MQ=60)
                                                                                    TTGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGA           >  SRR4302540.4810285/1‑94 (MQ=60)
                                                                                     TGCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAA          >  SRR4302540.2728858/1‑94 (MQ=60)
                                                                                      GCTCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAA         >  SRR4302540.1071397/1‑94 (MQ=60)
                                                                                        TCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAAT       <  SRR4302540.4115261/94‑1 (MQ=60)
                                                                                        TCGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAAT       >  SRR4302540.6088035/1‑94 (MQ=60)
                                                                                         CGACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAATT      <  SRR4302540.8226656/94‑1 (MQ=60)
                                                                                          GACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAATTA     <  SRR4302540.2095360/94‑1 (MQ=60)
                                                                                          GACGGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGCATTAGGTGCCCGCTCTCCTGAAAATTC     >  SRR4302540.2394342/1‑94 (MQ=60)
                                                                                             GGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAATTACCT  <  SRR4302540.1689844/94‑1 (MQ=60)
                                                                                             GGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAATTACCT  >  SRR4302540.5841053/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCGAAACCGGTACGCCGTAATTTTGTACCCGCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGATCACCTGAAAATTACCT  >  NC_000913/1483815‑1484001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑