Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,484,846 A→G A1254A (GCA→GCG)  hrpA → helicase, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,484,8460AG99.2% 775.3 / ‑3.6 246A1254A (GCA→GCG) hrpAhelicase, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (141/103);  total (141/105)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 268 total aligned reads displayed.

GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTA  >  NC_000913/1484756‑1484935
                                                                                          |                                                                                         
ggTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCcgcgcg                                                                                         <  1:3062412/93‑1 (MQ=255)
 gTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgt                                                                                        >  1:1650269/1‑93 (MQ=255)
 gTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgt                                                                                        >  1:3558700/1‑93 (MQ=255)
  ttGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtc                                                                                       >  1:4577918/1‑93 (MQ=255)
   tGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtcg                                                                                      <  2:5951307/93‑1 (MQ=255)
    ggtCCACATCGCGACCGGGCGCAGATGCTGAAAGTCGAAAACGTCCCGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtcgt                                                                                     <  2:8288150/91‑1 (MQ=255)
    gATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtcgt                                                                                     <  2:491809/93‑1 (MQ=255)
    gATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtcgt                                                                                     >  2:3119602/1‑93 (MQ=255)
     aTCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTg                                                                                    >  1:3313630/1‑93 (MQ=255)
      tCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGa                                                                                   >  1:7028720/1‑93 (MQ=255)
       ccACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAg                                                                                  <  1:6201254/93‑1 (MQ=255)
       ccACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAg                                                                                  >  2:5900289/1‑93 (MQ=255)
       ccACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAg                                                                                  <  2:4551829/93‑1 (MQ=255)
       ccACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAg                                                                                  >  2:4362973/1‑93 (MQ=255)
         ccaTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGa                                                                                <  1:954785/92‑1 (MQ=255)
          caTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGAAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAt                                                                               >  1:3424857/1‑93 (MQ=255)
          caTCACGACCGTACGCAGATGCTGAAAGTCGAAAACGTCCAGCGGGCGTGGCAGCAATGGATCAACAAACTGCCGCACGCGCGTCGTGAGGAt                                                                               <  2:8966305/93‑1 (MQ=255)
            tCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGa                                                                             >  2:4231370/1‑93 (MQ=255)
            tCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGa                                                                             <  1:4794474/93‑1 (MQ=255)
              gcgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAg                                                                           >  2:6428659/1‑93 (MQ=255)
               cgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGa                                                                          <  1:5647568/93‑1 (MQ=255)
                gACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCCATGGATCAACACACTGCCGCCCGCGCGCCGTGAGGATGAacac                                                                         >  2:6175960/1‑93 (MQ=255)
                gACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGAc                                                                         <  2:5292623/93‑1 (MQ=255)
                gACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGAGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGAc                                                                         >  1:3882737/1‑93 (MQ=255)
                 aCCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACg                                                                        >  1:9222543/1‑93 (MQ=255)
                    gTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGa                                                                     <  2:216792/93‑1 (MQ=255)
                    gTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGa                                                                     >  1:3667914/1‑93 (MQ=255)
                     tGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGaa                                                                    >  1:8631769/1‑93 (MQ=255)
                       cgcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCTTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAag                                                                  <  1:9861053/93‑1 (MQ=255)
                       cgcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAag                                                                  >  1:8074780/1‑93 (MQ=255)
                       cgcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAag                                                                  <  1:8180547/93‑1 (MQ=255)
                        gcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAaga                                                                 <  2:7972906/93‑1 (MQ=255)
                         cAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAagag                                                                >  2:811326/1‑93 (MQ=255)
                          aGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAgaga                                                               >  1:6120825/1‑93 (MQ=255)
                          aGATGCTCAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAgaga                                                               <  1:7836391/93‑1 (MQ=255)
                           gATGCTGAAAGTCGAAAACGTCCAGCAGGAGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAt                                                              >  1:7372284/1‑93 (MQ=255)
                            aTGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATc                                                             <  1:2061240/93‑1 (MQ=255)
                             tGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATcc                                                            <  2:7367442/93‑1 (MQ=255)
                              gCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCg                                                           >  1:251262/1‑93 (MQ=255)
                               cTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGt                                                          <  1:9070710/93‑1 (MQ=255)
                                tGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGTGTCGTGAGGATGAAGACGTGAAAGAGATCCGtt                                                         >  1:9671858/1‑93 (MQ=255)
                                tGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGtt                                                         >  1:8349940/1‑93 (MQ=255)
                                tGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGtt                                                         >  2:9229671/1‑93 (MQ=255)
                                 gAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTg                                                        >  1:283463/1‑93 (MQ=255)
                                 gAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTg                                                        >  2:6886268/1‑93 (MQ=255)
                                 gAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTg                                                        <  1:8499493/93‑1 (MQ=255)
                                 gAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTg                                                        >  1:3869713/1‑93 (MQ=255)
                                  aaaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTgg                                                       <  1:6461339/93‑1 (MQ=255)
                                   aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                      <  2:8720509/93‑1 (MQ=255)
                                    aGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgat                                                     >  1:1110374/1‑93 (MQ=255)
                                      tCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatga                                                   <  2:402680/93‑1 (MQ=255)
                                        gAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATa                                                 <  1:8134007/93‑1 (MQ=255)
                                        gAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATa                                                 >  2:540113/1‑93 (MQ=255)
                                         aaaaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATaa                                                >  2:5619748/1‑92 (MQ=255)
                                          aaaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATaga                                               >  2:4274239/1‑93 (MQ=255)
                                          aaaCGTCCAGAAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGGTGGATGATaga                                               >  2:3805236/1‑93 (MQ=255)
                                            aCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGACGACGTGAAAGAGATCCGTTGGATGATagaag                                             >  2:9830583/1‑93 (MQ=255)
                                            aCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaag                                             >  2:482521/1‑93 (MQ=255)
                                            aCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGCTGGATGATagaag                                             >  1:8953565/1‑93 (MQ=255)
                                              gTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAagag                                           >  1:5160335/1‑93 (MQ=255)
                                                ccAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGtt                                         <  2:2908744/93‑1 (MQ=255)
                                                ccAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGtt                                         <  2:2482536/93‑1 (MQ=255)
                                                ccAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGtt                                         <  1:572764/93‑1 (MQ=255)
                                                 cagcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTg                                        >  2:3530627/1‑93 (MQ=255)
                                                  agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       >  2:5356522/1‑93 (MQ=255)
                                                  agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       <  1:1818803/93‑1 (MQ=255)
                                                      ggCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgt                                   >  2:8691351/1‑93 (MQ=255)
                                                      ggCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgt                                   >  2:7700133/1‑93 (MQ=255)
                                                        cGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgtta                                 <  2:3766093/93‑1 (MQ=255)
                                                         gTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAGAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttag                                <  2:2709927/93‑1 (MQ=255)
                                                          tGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagt                               <  2:4754868/93‑1 (MQ=255)
                                                            gcagcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagtta                             <  2:1402063/93‑1 (MQ=255)
                                                              agcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACt                           >  1:6058286/1‑93 (MQ=255)
                                                               gcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACtt                          >  2:9685084/1‑93 (MQ=255)
                                                                 aaTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCg                        >  1:2231462/1‑93 (MQ=255)
                                                                 aaTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCg                        >  2:3917432/1‑93 (MQ=255)
                                                                 aaTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCg                        >  1:6798486/1‑93 (MQ=255)
                                                                  aTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGc                       >  2:5105698/1‑93 (MQ=255)
                                                                   tGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCt                      >  1:7504557/1‑93 (MQ=255)
                                                                     gATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTca                    <  2:2975949/93‑1 (MQ=255)
                                                                       tCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaac                  >  2:9425001/1‑93 (MQ=255)
                                                                       tCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaac                  >  2:2060822/1‑93 (MQ=255)
                                                                       tCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaac                  <  1:259951/93‑1 (MQ=255)
                                                                          acaaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCaacaac               <  1:9395977/93‑1 (MQ=255)
                                                                            aaaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACtg             >  1:1545582/1‑92 (MQ=255)
                                                                             aaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTg            <  2:8772243/93‑1 (MQ=255)
                                                                              aCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTgg           <  2:4412009/93‑1 (MQ=255)
                                                                               cTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGt          >  2:7219322/1‑93 (MQ=255)
                                                                               cTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGt          >  1:10044158/1‑93 (MQ=255)
                                                                                tGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTa         <  2:5445456/93‑1 (MQ=255)
                                                                                tGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTa         <  2:9733925/93‑1 (MQ=255)
                                                                                tGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTa         <  1:4116202/93‑1 (MQ=255)
                                                                                 gccgccCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAc        <  1:5351690/93‑1 (MQ=255)
                                                                                    gcccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGcc     >  2:9892469/1‑93 (MQ=255)
                                                                                     cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCACCAACTAGCTACGCCt    >  2:1073371/1‑93 (MQ=255)
                                                                                     cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCt    >  2:1764328/1‑93 (MQ=255)
                                                                                     cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCt    >  2:7558816/1‑93 (MQ=255)
                                                                                      ccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCtt   >  2:32400/1‑93 (MQ=255)
                                                                                       cgcgcgTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTa  >  2:1099390/1‑93 (MQ=255)
                                                                                       cgcgcgTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTa  >  2:5005547/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTA  >  NC_000913/1484756‑1484935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCG  >  NC_000913/1484756‑1484939
                                                                                          |                                                                                             
GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGT                                                                                            >  SRR4302540.3597580/1‑94 (MQ=60)
GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGT                                                                                            <  SRR4302540.5735293/94‑1 (MQ=60)
GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGT                                                                                            >  SRR4302540.4415283/1‑94 (MQ=60)
GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGT                                                                                            <  SRR4302540.3096010/94‑1 (MQ=60)
GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGT                                                                                            >  SRR4302540.1668267/1‑94 (MQ=60)
 GTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTC                                                                                           >  SRR4302540.8368477/1‑94 (MQ=60)
 GTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTC                                                                                           >  SRR4302540.4627288/1‑94 (MQ=60)
  TTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCG                                                                                          >  SRR4302540.1593709/1‑94 (MQ=60)
   TGATCCACATCGCGACCGTGCGCAGATGCTGAAAGCCGAAAACGTCCAGCAGGCGTTGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGG                                                                                         >  SRR4302540.4517009/1‑94 (MQ=60)
    GATCCACATCGCGACCGTGCGCAGATGCTTAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCCCCCGCGCGTCGTG                                                                                        >  SRR4302540.8570821/1‑94 (MQ=60)
    GATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTG                                                                                        >  SRR4302540.3349940/1‑94 (MQ=60)
     ATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGA                                                                                       >  SRR4302540.7106815/1‑94 (MQ=60)
     ATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGA                                                                                       >  SRR4302540.3659118/1‑94 (MQ=60)
      TCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAG                                                                                      <  SRR4302540.3860693/94‑1 (MQ=60)
      TCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAG                                                                                      <  SRR4302540.8204292/94‑1 (MQ=60)
      TCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAG                                                                                      <  SRR4302540.7941403/94‑1 (MQ=60)
       CCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGG                                                                                     <  SRR4302540.6270284/94‑1 (MQ=60)
       CCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGG                                                                                     <  SRR4302540.6270285/94‑1 (MQ=60)
       CCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGG                                                                                     <  SRR4302540.9691060/94‑1 (MQ=60)
        CACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGA                                                                                    >  SRR4302540.9162147/1‑94 (MQ=60)
         CCATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAT                                                                                   <  SRR4302540.964685/94‑1 (MQ=60)
         ACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAT                                                                                   <  SRR4302540.1047218/94‑1 (MQ=60)
         ACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAT                                                                                   >  SRR4302540.4688198/1‑94 (MQ=60)
         ACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGAAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAT                                                                                   >  SRR4302540.3462090/1‑94 (MQ=60)
           ATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGA                                                                                 >  SRR4302540.3797388/1‑94 (MQ=60)
           ATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGAGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGA                                                                                 >  SRR4302540.8033596/1‑94 (MQ=60)
            TCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAA                                                                                <  SRR4302540.4845763/94‑1 (MQ=60)
             CGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAG                                                                               <  SRR4302540.1748820/94‑1 (MQ=60)
              GCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGA                                                                              <  SRR4302540.8484316/94‑1 (MQ=60)
               CGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGAC                                                                             <  SRR4302540.5709730/94‑1 (MQ=60)
               CGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGAC                                                                             <  SRR4302540.6319273/94‑1 (MQ=60)
               CGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGAGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGAC                                                                             >  SRR4302540.3924923/1‑94 (MQ=60)
                GACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACG                                                                            >  SRR4302540.9333137/1‑94 (MQ=60)
                   CGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGA                                                                         >  SRR4302540.3708039/1‑94 (MQ=60)
                    GTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAA                                                                        >  SRR4302540.8732033/1‑94 (MQ=60)
                     TGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAA                                                                       >  SRR4302540.3364023/1‑94 (MQ=60)
                     TGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAA                                                                       >  SRR4302540.5770327/1‑94 (MQ=60)
                      GCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAG                                                                      >  SRR4302540.8168479/1‑94 (MQ=60)
                      GCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAG                                                                      <  SRR4302540.9549793/94‑1 (MQ=60)
                       CGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCTTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGA                                                                     <  SRR4302540.9976265/94‑1 (MQ=60)
                       CGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGA                                                                     >  SRR4302540.6147342/1‑94 (MQ=60)
                       CGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGA                                                                     >  SRR4302540.6147341/1‑94 (MQ=60)
                       CGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGA                                                                     <  SRR4302540.8275567/94‑1 (MQ=60)
                        GCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAG                                                                    >  SRR4302540.4028134/1‑94 (MQ=60)
                         CAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGA                                                                   >  SRR4302540.5514208/1‑94 (MQ=60)
                         CAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGA                                                                   >  SRR4302540.6188996/1‑94 (MQ=60)
                          AGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAC                                                                  >  SRR4302540.7485965/1‑94 (MQ=60)
                          AGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGACGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAT                                                                  >  SRR4302540.6802213/1‑94 (MQ=60)
                          AGATGCTGAAAGTCGAAAACGTCCAGCAGGAGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAT                                                                  >  SRR4302540.7454461/1‑94 (MQ=60)
                          AGATGCTCAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAT                                                                  <  SRR4302540.7927948/94‑1 (MQ=60)
                           GATGCTGAAAGTCGAAGCCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATC                                                                 <  SRR4302540.9066250/94‑1 (MQ=60)
                            ATGCTGAAAGTCGAAAGCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCC                                                                <  SRR4302540.8841416/94‑1 (MQ=60)
                            ATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCC                                                                <  SRR4302540.2083423/94‑1 (MQ=60)
                            ATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCC                                                                <  SRR4302540.3920413/94‑1 (MQ=60)
                            ATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCC                                                                <  SRR4302540.3373209/94‑1 (MQ=60)
                            ATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCC                                                                >  SRR4302540.9888677/1‑94 (MQ=60)
                             TGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCG                                                               >  SRR4302540.253694/1‑94 (MQ=60)
                              GCTGACAGTCGAAAACGTCCAGCAGGCGTGGCAGCACTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGT                                                              >  SRR4302540.6117632/1‑94 (MQ=60)
                              GCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGT                                                              >  SRR4302540.8363370/1‑94 (MQ=60)
                              GCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGT                                                              >  SRR4302540.6117630/1‑94 (MQ=60)
                              GCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGT                                                              <  SRR4302540.3224945/94‑1 (MQ=60)
                               CTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGTGTCGTGAGGATGAAGACGTGAAAGAGATCCGTT                                                             >  SRR4302540.9785679/1‑94 (MQ=60)
                               CTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTT                                                             >  SRR4302540.8446852/1‑94 (MQ=60)
                               CTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTT                                                             <  SRR4302540.9179644/94‑1 (MQ=60)
                                TGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTG                                                            >  SRR4302540.286250/1‑94 (MQ=60)
                                TGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTG                                                            >  SRR4302540.3911779/1‑94 (MQ=60)
                                 GAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGG                                                           <  SRR4302540.8598259/94‑1 (MQ=60)
                                  AAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGA                                                          <  SRR4302540.6533250/94‑1 (MQ=60)
                                   AAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGAT                                                         >  SRR4302540.1121859/1‑94 (MQ=60)
                                    AGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATG                                                        <  SRR4302540.634704/94‑1 (MQ=60)
                                     GTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGA                                                       >  SRR4302540.2000555/1‑94 (MQ=60)
                                        GAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAG                                                    <  SRR4302540.8228413/94‑1 (MQ=60)
                                        GAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAG                                                    >  SRR4302540.8709980/1‑94 (MQ=60)
                                         AAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGA                                                   <  SRR4302540.1938062/94‑1 (MQ=60)
                                          AAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAA                                                  <  SRR4302540.10076481/94‑1 (MQ=60)
                                          AAACGTCCAGCACGCGTGGCAGCAATGGATCAACAAACTCCCGCCCGCGCGTCGTGAGGATGAAGCCGTGAAAGAGATCCGTTGGATGATAGAA                                                  <  SRR4302540.765304/94‑1 (MQ=60)
                                           AACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGCTGGATGATAGAAG                                                 >  SRR4302540.9059202/1‑94 (MQ=60)
                                            ACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGA                                                <  SRR4302540.2585969/94‑1 (MQ=60)
                                             CGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAG                                               >  SRR4302540.5216428/1‑94 (MQ=60)
                                             CGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAG                                               <  SRR4302540.3702946/94‑1 (MQ=60)
                                             CGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAG                                               <  SRR4302540.5099633/94‑1 (MQ=60)
                                              GTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGT                                              >  SRR4302540.8200212/1‑94 (MQ=60)
                                                CCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTG                                            <  SRR4302540.578981/94‑1 (MQ=60)
                                                  AGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCG                                          <  SRR4302540.1838674/94‑1 (MQ=60)
                                                   GTAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGC                                         <  SRR4302540.2890160/94‑1 (MQ=60)
                                                       GCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTA                                     >  SRR4302540.8228866/1‑94 (MQ=60)
                                                         GTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGT                                   >  SRR4302540.9407027/1‑94 (MQ=60)
                                                          TGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTT                                  <  SRR4302540.3930191/94‑1 (MQ=60)
                                                             CAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACT                               >  SRR4302540.6125619/1‑94 (MQ=60)
                                                               GCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTC                             >  SRR4302540.7299149/1‑94 (MQ=60)
                                                               GCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTC                             <  SRR4302540.6769517/94‑1 (MQ=60)
                                                                CAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCG                            >  SRR4302540.2255488/1‑94 (MQ=60)
                                                                CAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCG                            >  SRR4302540.6873690/1‑94 (MQ=60)
                                                                 AATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGC                           >  SRR4302540.1588457/1‑94 (MQ=60)
                                                                  ATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCTCT                          >  SRR4302540.7588427/1‑94 (MQ=60)
                                                                  ATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCT                          >  SRR4302540.7588428/1‑94 (MQ=60)
                                                                    GGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCA                        <  SRR4302540.2817726/94‑1 (MQ=60)
                                                                       TCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACA                     <  SRR4302540.2107152/94‑1 (MQ=60)
                                                                       TCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACA                     <  SRR4302540.2479826/94‑1 (MQ=60)
                                                                       TCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACA                     <  SRR4302540.262480/94‑1 (MQ=60)
                                                                         AACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAAC                   >  SRR4302540.7284784/1‑94 (MQ=60)
                                                                          ACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACT                  <  SRR4302540.9507783/94‑1 (MQ=60)
                                                                          ACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACT                  >  SRR4302540.9579627/1‑94 (MQ=60)
                                                                          ACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACT                  <  SRR4302540.9049923/94‑1 (MQ=60)
                                                                           CAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTT                 <  SRR4302540.5180341/94‑1 (MQ=60)
                                                                           CAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTG                 >  SRR4302540.1562639/1‑94 (MQ=60)
                                                                            AAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTG                >  SRR4302540.346506/1‑94 (MQ=60)
                                                                             AACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGG               <  SRR4302540.2099882/94‑1 (MQ=60)
                                                                             AACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGG               <  SRR4302540.3139343/94‑1 (MQ=60)
                                                                              ACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGT              >  SRR4302540.10160928/1‑94 (MQ=60)
                                                                              ACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGT              >  SRR4302540.5243386/1‑94 (MQ=60)
                                                                               CTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTA             <  SRR4302540.7054489/94‑1 (MQ=60)
                                                                                TGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAC            <  SRR4302540.7741126/94‑1 (MQ=60)
                                                                                TGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAC            <  SRR4302540.4160642/94‑1 (MQ=60)
                                                                                TGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAC            >  SRR4302540.1292065/1‑94 (MQ=60)
                                                                                TGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAC            >  SRR4302540.1292066/1‑94 (MQ=60)
                                                                                TGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAC            <  SRR4302540.9194092/94‑1 (MQ=60)
                                                                                TGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAC            >  SRR4302540.2635906/1‑94 (MQ=60)
                                                                                TGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAC            >  SRR4302540.5356284/1‑94 (MQ=60)
                                                                                 GCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACG           <  SRR4302540.5410431/94‑1 (MQ=60)
                                                                                     CCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTT       >  SRR4302540.9859431/1‑94 (MQ=60)
                                                                                      CCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTA      <  SRR4302540.9481960/94‑1 (MQ=60)
                                                                                        GCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATC    >  SRR4302540.1985572/1‑94 (MQ=60)
                                                                                        GCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATC    <  SRR4302540.4016188/94‑1 (MQ=60)
                                                                                         CGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCC   >  SRR4302540.1338175/1‑94 (MQ=60)
                                                                                         CGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCC   <  SRR4302540.4063112/94‑1 (MQ=60)
                                                                                          GCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCG  <  SRR4302540.5883318/94‑1 (MQ=60)
                                                                                          GCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCG  >  SRR4302540.8708765/1‑94 (MQ=60)
                                                                                          |                                                                                             
GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATCCG  >  NC_000913/1484756‑1484939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑