Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,484,846 A→G A1254A (GCA→GCG)  hrpA → helicase, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,484,8460AG99.4% 558.8 / NA 177A1254A (GCA→GCG) hrpAhelicase, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (86/90);  minor base C (1/0);  total (87/90)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

TTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTA  >  NC_000913/1484758‑1484935
                                                                                        |                                                                                         
  gATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtcgt                                                                                     >  2:2878783/1‑93 (MQ=255)
  gATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtcgg                                                                                     >  2:4474493/1‑92 (MQ=255)
   aTCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTg                                                                                    >  2:4966225/1‑93 (MQ=255)
    tCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGa                                                                                   <  2:1765994/93‑1 (MQ=255)
    tCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGa                                                                                   >  1:5100504/1‑93 (MQ=255)
    tCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGa                                                                                   <  1:4733080/93‑1 (MQ=255)
    tCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGa                                                                                   >  1:6146547/1‑93 (MQ=255)
       acaTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGa                                                                                >  1:6011041/1‑93 (MQ=255)
        caTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAt                                                                               >  1:5861533/1‑93 (MQ=255)
            gcgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAg                                                                           <  2:3218297/93‑1 (MQ=255)
            gcgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAg                                                                           <  1:1809774/93‑1 (MQ=255)
              gACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGAc                                                                         >  2:3037081/1‑93 (MQ=255)
              gACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGAGTGGCAGCAATGGATCAACCAACTGCCGCCCGCGCGTCGTGAGGATGAAGAc                                                                         >  2:2182671/1‑93 (MQ=255)
               aCCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACg                                                                        <  2:2043169/93‑1 (MQ=255)
                ccGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGt                                                                       <  2:5543342/93‑1 (MQ=255)
                  gTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGa                                                                     <  2:1215791/93‑1 (MQ=255)
                  gTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGa                                                                     <  1:2671126/93‑1 (MQ=255)
                  gTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGAGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGa                                                                     >  1:2743209/1‑93 (MQ=255)
                    gcgcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGaaa                                                                   >  1:6202112/1‑93 (MQ=255)
                     cgcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAag                                                                  <  1:3015191/93‑1 (MQ=255)
                      gcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAaga                                                                 <  1:1271761/93‑1 (MQ=255)
                      gcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAaga                                                                 >  2:3826694/1‑93 (MQ=255)
                      gcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAaga                                                                 >  1:6171640/1‑93 (MQ=255)
                      gcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAaga                                                                 >  1:3227330/1‑93 (MQ=255)
                       cAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAagag                                                                >  1:976642/1‑93 (MQ=255)
                        aGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGCCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAgaga                                                               <  1:2949527/93‑1 (MQ=255)
                         gATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAt                                                              <  2:1997841/93‑1 (MQ=255)
                         gATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAt                                                              >  2:36839/1‑93 (MQ=255)
                          aTGCTGAAAGTCGAAAACGTCCAGCAGGAGTGGCAGCAATGGATCAACAAACTGCCGCCCGCCCGTCGTGAGGATGAAGACGTGAAAGAGATc                                                             >  1:4930156/1‑93 (MQ=255)
                           tGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATcc                                                            <  2:684338/93‑1 (MQ=255)
                           tGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATcc                                                            >  1:1624474/1‑93 (MQ=255)
                           tGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATcc                                                            <  2:5483477/93‑1 (MQ=255)
                            gCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCg                                                           <  1:2135344/93‑1 (MQ=255)
                                 aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                      <  1:5578062/93‑1 (MQ=255)
                                 aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                      >  1:1563824/1‑93 (MQ=255)
                                 aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                      >  2:5465223/1‑93 (MQ=255)
                                   gTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatg                                                    >  2:887944/1‑93 (MQ=255)
                                    tCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatgt                                                   >  2:1576370/1‑92 (MQ=255)
                                    tCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatga                                                   <  1:4340264/93‑1 (MQ=255)
                                    tCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatga                                                   <  2:463735/93‑1 (MQ=255)
                                    gcGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatga                                                   <  2:4664543/92‑1 (MQ=255)
                                      gAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATa                                                 >  1:3610757/1‑93 (MQ=255)
                                       aaaaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATag                                                <  2:5029432/93‑1 (MQ=255)
                                         aaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaa                                              >  1:4597202/1‑93 (MQ=255)
                                         aaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaa                                              <  1:4172236/93‑1 (MQ=255)
                                         aaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaa                                              >  2:2476289/1‑93 (MQ=255)
                                          aCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaag                                             <  1:1183937/93‑1 (MQ=255)
                                          aCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaag                                             <  2:1136236/93‑1 (MQ=255)
                                          aCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaag                                             <  1:50383/93‑1 (MQ=255)
                                           cGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaaga                                            >  2:4933020/1‑93 (MQ=255)
                                           cGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaaga                                            <  2:1952516/93‑1 (MQ=255)
                                            gTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAagag                                           >  1:4052248/1‑93 (MQ=255)
                                            gTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAagag                                           <  1:147520/93‑1 (MQ=255)
                                            gTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAagag                                           >  1:5353089/1‑93 (MQ=255)
                                               cagcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTg                                        >  2:3622021/1‑93 (MQ=255)
                                                agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       <  1:1696992/93‑1 (MQ=255)
                                                 gcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgcg                                      <  1:39483/93‑1 (MQ=255)
                                                 gcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgcg                                      >  1:5829152/1‑93 (MQ=255)
                                                   agGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGcgcg                                    >  2:1740369/1‑93 (MQ=255)
                                                    ggCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgt                                   <  2:1055257/93‑1 (MQ=255)
                                                     gCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgtt                                  >  1:1191296/1‑93 (MQ=255)
                                                     gCGTGGCAGCAATGCATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgtt                                  <  2:576688/93‑1 (MQ=255)
                                                      cGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgtta                                 <  1:3701232/93‑1 (MQ=255)
                                                       gTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttag                                >  2:4825076/1‑93 (MQ=255)
                                                       gTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAGCCGTTGGATGATAGAAGAGTCGGGCggtag                                >  2:3445662/1‑93 (MQ=255)
                                                        tGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagt                               <  2:1891604/93‑1 (MQ=255)
                                                        tGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagt                               <  1:3469828/93‑1 (MQ=255)
                                                        tGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagt                               >  2:388334/1‑93 (MQ=255)
                                                         ggCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagtt                              <  2:6205946/93‑1 (MQ=255)
                                                          gcagcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagtta                             >  2:1659850/1‑93 (MQ=255)
                                                            agcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACt                           >  1:1617720/1‑93 (MQ=255)
                                                            agcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACt                           >  2:4052794/1‑93 (MQ=255)
                                                             gcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACtt                          >  1:607934/1‑93 (MQ=255)
                                                              caATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTc                         <  2:5163070/93‑1 (MQ=255)
                                                                aTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGc                       <  1:2714363/93‑1 (MQ=255)
                                                                aTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGc                       >  1:3980070/1‑93 (MQ=255)
                                                                 tGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCt                      <  2:5562697/93‑1 (MQ=255)
                                                                 tGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCt                      >  1:4895834/1‑93 (MQ=255)
                                                                  ggATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTc                     >  2:2521360/1‑93 (MQ=255)
                                                                  ggATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTc                     >  1:5511762/1‑93 (MQ=255)
                                                                    aTCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaa                   >  1:3351586/1‑93 (MQ=255)
                                                                     tCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaac                  <  2:5394199/93‑1 (MQ=255)
                                                                        acaaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCaacaac               <  1:75765/93‑1 (MQ=255)
                                                                        acaaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCaacaac               <  1:1939829/93‑1 (MQ=255)
                                                                        acaaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCaacaac               <  1:2342071/93‑1 (MQ=255)
                                                                         caaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACt              <  2:5134328/93‑1 (MQ=255)
                                                                          aaaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACtt             >  2:2381850/1‑93 (MQ=255)
                                                                           aaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTg            >  2:1297768/1‑93 (MQ=255)
                                                                             cTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGt          >  2:3748771/1‑93 (MQ=255)
                                                                             cTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGt          <  1:2185652/93‑1 (MQ=255)
                                                                              tGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTa         <  2:2499880/93‑1 (MQ=255)
                                                                              tGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTa         <  1:3916823/93‑1 (MQ=255)
                                                                               gccgccCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAc        <  1:5753410/93‑1 (MQ=255)
                                                                                ccgcccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACg       >  1:5428324/1‑93 (MQ=255)
                                                                                 cgcccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGc      >  2:2263815/1‑93 (MQ=255)
                                                                                 cgcccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGc      <  1:4670556/93‑1 (MQ=255)
                                                                                  gcccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGcc     >  1:640606/1‑93 (MQ=255)
                                                                                   cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCt    >  1:1980211/1‑93 (MQ=255)
                                                                                   cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCt    <  2:3335976/93‑1 (MQ=255)
                                                                                     cgcgcgTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTa  <  1:2430796/93‑1 (MQ=255)
                                                                                        |                                                                                         
TTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTA  >  NC_000913/1484758‑1484935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATC  >  NC_000913/1484753‑1484937
                                                                                             |                                                                                           
GGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCG                                                                                             <  SRR4302533.5338006/94‑1 (MQ=60)
     TTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCG                                                                                        <  SRR4302533.5880266/94‑1 (MQ=60)
        ATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGA                                                                                     >  SRR4302533.5485117/1‑94 (MQ=60)
        ATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGA                                                                                     >  SRR4302533.6183552/1‑94 (MQ=60)
        ATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGA                                                                                     >  SRR4302533.1501067/1‑94 (MQ=60)
        ATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGA                                                                                     >  SRR4302533.5131314/1‑94 (MQ=60)
        ATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGA                                                                                     >  SRR4302533.4226710/1‑94 (MQ=60)
         TCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAG                                                                                    <  SRR4302533.2520306/94‑1 (MQ=60)
         TCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAG                                                                                    <  SRR4302533.4761772/94‑1 (MQ=60)
          CCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGG                                                                                   >  SRR4302533.6293023/1‑94 (MQ=60)
          CCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCCCCCGCGCGTCGTGAGG                                                                                   >  SRR4302533.5722947/1‑94 (MQ=60)
          CCAC‑‑CGCGAGCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAT                                                                                 <  SRR4302533.5395942/94‑1 (MQ=60)
           CACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGA                                                                                  >  SRR4302533.6047458/1‑94 (MQ=60)
            ACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAT                                                                                 <  SRR4302533.6210655/94‑1 (MQ=60)
            ACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAT                                                                                 >  SRR4302533.5896973/1‑94 (MQ=60)
             CATCTCGACCGTGCGCAGATGCTGAAATTCGAAAGCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATG                                                                                <  SRR4302533.563532/94‑1 (MQ=60)
             CATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATG                                                                                >  SRR4302533.2749175/1‑94 (MQ=60)
                 GCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGA                                                                            <  SRR4302533.1820788/94‑1 (MQ=60)
                      CGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCGCGCGCGTCGTGAGGATGACGACGGGA                                                                       >  SRR4302533.4163675/1‑94 (MQ=60)
                      CGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGAGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGA                                                                       >  SRR4302533.2760030/1‑94 (MQ=60)
                       GTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAA                                                                      <  SRR4302533.2687509/94‑1 (MQ=60)
                        TGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAA                                                                     >  SRR4302533.6239310/1‑94 (MQ=60)
                         GCGCAGATGCTGAAAATCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAG                                                                    <  SRR4302533.1671695/94‑1 (MQ=60)
                          CGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGA                                                                   >  SRR4302533.3247227/1‑94 (MQ=60)
                          CGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGA                                                                   <  SRR4302533.5403678/94‑1 (MQ=60)
                          CGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGA                                                                   <  SRR4302533.3033632/94‑1 (MQ=60)
                          CGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGA                                                                   >  SRR4302533.6208728/1‑94 (MQ=60)
                           GCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAG                                                                  <  SRR4302533.2419166/94‑1 (MQ=60)
                           GCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAG                                                                  <  SRR4302533.1279368/94‑1 (MQ=60)
                           GCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAG                                                                  >  SRR4302533.982372/1‑94 (MQ=60)
                            CAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGA                                                                 >  SRR4302533.3942130/1‑94 (MQ=60)
                             AGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGCCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGAT                                                                <  SRR4302533.2967566/94‑1 (MQ=60)
                              GATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATC                                                               >  SRR4302533.4960010/1‑94 (MQ=60)
                              GATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATC                                                               <  SRR4302533.6051388/94‑1 (MQ=60)
                              GATGCTGAAAGTCGAAAACGTCCAGCAGGAGTGGCAGCAATGGATCAACAAACTGCCGCCCGCCCGTCGTGAGGATGAAGACGTGAAAGAGATC                                                               >  SRR4302533.4960011/1‑94 (MQ=60)
                               ATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCC                                                              >  SRR4302533.1634313/1‑94 (MQ=60)
                                 GCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGT                                                            <  SRR4302533.2148510/94‑1 (MQ=60)
                                 GCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGT                                                            <  SRR4302533.3725013/94‑1 (MQ=60)
                                     AAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGA                                                        >  SRR4302533.1573238/1‑94 (MQ=60)
                                      AAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGAT                                                       <  SRR4302533.5611774/94‑1 (MQ=60)
                                       AGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATG                                                      <  SRR4302533.559588/94‑1 (MQ=60)
                                       AGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATG                                                      >  SRR4302533.5064388/1‑94 (MQ=60)
                                         TCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGAT                                                    <  SRR4302533.4366425/94‑1 (MQ=60)
                                          CGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATA                                                   >  SRR4302533.3632980/1‑94 (MQ=60)
                                           GAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAG                                                  <  SRR4302533.3005704/94‑1 (MQ=60)
                                            AAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGA                                                 >  SRR4302533.2898809/1‑94 (MQ=60)
                                             AAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAA                                                >  SRR4302533.4625050/1‑94 (MQ=60)
                                              AACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAG                                               <  SRR4302533.4197518/94‑1 (MQ=60)
                                               ACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGA                                              <  SRR4302533.71502/94‑1 (MQ=60)
                                               ACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGA                                              <  SRR4302533.50728/94‑1 (MQ=60)
                                               ACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGA                                              <  SRR4302533.1191043/94‑1 (MQ=60)
                                                CGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAG                                             >  SRR4302533.5385216/1‑94 (MQ=60)
                                                CGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAG                                             >  SRR4302533.4023431/1‑94 (MQ=60)
                                                CGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAG                                             >  SRR4302533.4076926/1‑94 (MQ=60)
                                                 GTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGT                                            <  SRR4302533.148464/94‑1 (MQ=60)
                                                 GTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGT                                            <  SRR4302533.3341450/94‑1 (MQ=60)
                                                     AGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCG                                        <  SRR4302533.1707270/94‑1 (MQ=60)
                                                     AGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCG                                        >  SRR4302533.5864376/1‑94 (MQ=60)
                                                      GCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGC                                       <  SRR4302533.39745/94‑1 (MQ=60)
                                                         GGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTT                                    >  SRR4302533.1198454/1‑94 (MQ=60)
                                                         GGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTT                                    >  SRR4302533.4699463/1‑94 (MQ=60)
                                                           CGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAG                                  <  SRR4302533.1892878/94‑1 (MQ=60)
                                                           CGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAG                                  >  SRR4302533.3166164/1‑94 (MQ=60)
                                                           CGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAG                                  <  SRR4302533.3723962/94‑1 (MQ=60)
                                                             TGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTT                                <  SRR4302533.3549530/94‑1 (MQ=60)
                                                             TGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTT                                <  SRR4302533.3491247/94‑1 (MQ=60)
                                                                CAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACT                             >  SRR4302533.1627521/1‑94 (MQ=60)
                                                                 AGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTT                            >  SRR4302533.611511/1‑94 (MQ=60)
                                                                  GCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTC                           <  SRR4302533.5284859/94‑1 (MQ=60)
                                                                  GCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTC                           <  SRR4302533.1615633/94‑1 (MQ=60)
                                                                   CAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCG                          <  SRR4302533.1789769/94‑1 (MQ=60)
                                                                    AATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGC                         >  SRR4302533.4004303/1‑94 (MQ=60)
                                                                     ATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCT                        <  SRR4302533.2731030/94‑1 (MQ=60)
                                                                     ATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCT                        <  SRR4302533.5966088/94‑1 (MQ=60)
                                                                     ATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCT                        >  SRR4302533.4925500/1‑94 (MQ=60)
                                                                      TGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTC                       >  SRR4302533.5545037/1‑94 (MQ=60)
                                                                        GATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAA                     >  SRR4302533.3372289/1‑94 (MQ=60)
                                                                         ATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAAC                    >  SRR4302533.4874049/1‑94 (MQ=60)
                                                                         ATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAAC                    <  SRR4302533.6177685/94‑1 (MQ=60)
                                                                         ATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAAC                    <  SRR4302533.195344/94‑1 (MQ=60)
                                                                             ACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACT                <  SRR4302533.2356571/94‑1 (MQ=60)
                                                                             ACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACT                <  SRR4302533.76257/94‑1 (MQ=60)
                                                                             ACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACT                <  SRR4302533.1951661/94‑1 (MQ=60)
                                                                               AAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTG              <  SRR4302533.3644809/94‑1 (MQ=60)
                                                                                AACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGG             >  SRR4302533.1327746/1‑94 (MQ=60)
                                                                                AACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGG             <  SRR4302533.2322775/94‑1 (MQ=60)
                                                                                  CTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTA           <  SRR4302533.4379177/94‑1 (MQ=60)
                                                                                  CTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTA           <  SRR4302533.2199125/94‑1 (MQ=60)
                                                                                   TGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAC          <  SRR4302533.3940747/94‑1 (MQ=60)
                                                                                    GCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACG         >  SRR4302533.5461016/1‑94 (MQ=60)
                                                                                    GCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACG         <  SRR4302533.5788121/94‑1 (MQ=60)
                                                                                     CCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGC        >  SRR4302533.1265797/1‑94 (MQ=60)
                                                                                      CGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCC       >  SRR4302533.1535373/1‑94 (MQ=60)
                                                                                      CGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCC       >  SRR4302533.644373/1‑94 (MQ=60)
                                                                                      CGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCC       <  SRR4302533.4698860/94‑1 (MQ=60)
                                                                                       GCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCT      >  SRR4302533.1992336/1‑94 (MQ=60)
                                                                                        CCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTT     <  SRR4302533.982017/94‑1 (MQ=60)
                                                                                         CCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTA    >  SRR4302533.2187082/1‑94 (MQ=60)
                                                                                          CGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTAT   <  SRR4302533.2445821/94‑1 (MQ=60)
                                                                                           GCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATC  <  SRR4302533.300279/94‑1 (MQ=60)
                                                                                           GCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATC  <  SRR4302533.5071373/94‑1 (MQ=60)
                                                                                             |                                                                                           
GGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTATC  >  NC_000913/1484753‑1484937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑