Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,493,567 T→C F86L (TTT→CTT)  b1423 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,493,5670TC99.5% 675.6 / ‑5.0 212F86L (TTT→CTT) b1423hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (98/113);  total (98/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01

Only 100 of 225 total aligned reads displayed.

TAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGAT  >  NC_000913/1493475‑1493656
                                                                                            |                                                                                         
  aaggCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGctt                                                                                         <  1:4667148/90‑1 (MQ=255)
  aaTGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGctt                                                                                         >  1:9490000/1‑93 (MQ=255)
   aTGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTg                                                                                        >  2:3089967/1‑93 (MQ=255)
    tGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGc                                                                                       >  1:1163460/1‑93 (MQ=255)
    tGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGc                                                                                       >  2:2219783/1‑93 (MQ=255)
     gCAGACGCGCTGGCGCGACTGAATGTTGAACGTCATGGGGAGATTCGCGGTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGAGGCTTggc                                                                                      >  2:439120/1‑91 (MQ=255)
      cAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCa                                                                                     <  2:9497586/93‑1 (MQ=255)
      cAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCa                                                                                     <  2:7126958/93‑1 (MQ=255)
        gACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCAtt                                                                                   >  2:8148609/1‑93 (MQ=255)
        gACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCAtt                                                                                   >  2:2282093/1‑93 (MQ=255)
         aCGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTa                                                                                  >  2:3250231/1‑93 (MQ=255)
         aCGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTa                                                                                  >  2:171308/1‑93 (MQ=255)
           gAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATg                                                                                >  1:8572292/1‑93 (MQ=255)
             tcTGGCGCGACAGAATTTTGAACGTCGGGGCGCTATGCGCGTTGGGACTG‑‑CAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGa                                                                            <  2:5013531/92‑1 (MQ=255)
             gCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATggg                                                                              <  1:2589533/93‑1 (MQ=255)
              cTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATgggg                                                                             <  2:6827511/93‑1 (MQ=255)
                ggCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGAt                                                                           <  2:301297/93‑1 (MQ=255)
                 gcgcGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATg                                                                          <  1:3592655/93‑1 (MQ=255)
                   gcgACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTa                                                                        <  2:9155018/93‑1 (MQ=255)
                    cgACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTAc                                                                       <  1:3893398/93‑1 (MQ=255)
                     gACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTAcc                                                                      <  1:9106395/93‑1 (MQ=255)
                     gACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTAcc                                                                      >  1:9699068/1‑93 (MQ=255)
                     gACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTAcc                                                                      >  1:9883604/1‑93 (MQ=255)
                      aCTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTAccc                                                                     >  2:3694147/1‑93 (MQ=255)
                      aCTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTAccc                                                                     <  2:6866187/93‑1 (MQ=255)
                       cTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCg                                                                    <  1:3184227/93‑1 (MQ=255)
                       cTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCg                                                                    <  2:641179/93‑1 (MQ=255)
                         gAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGt                                                                  <  2:8714178/93‑1 (MQ=255)
                         gAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGt                                                                  >  2:2606304/1‑93 (MQ=255)
                          aaTGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTg                                                                 <  2:9341951/93‑1 (MQ=255)
                             gTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGc                                                              >  1:8496979/1‑93 (MQ=255)
                              ttGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCt                                                             <  2:3301315/93‑1 (MQ=255)
                               tGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTa                                                            <  1:3664200/93‑1 (MQ=255)
                                   cGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTAc                                                        >  1:5654039/1‑93 (MQ=255)
                                   cGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTAc                                                        <  1:3816721/93‑1 (MQ=255)
                                    gTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACg                                                       >  1:237483/1‑93 (MQ=255)
                                    gTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACg                                                       >  2:6210082/1‑93 (MQ=255)
                                    gTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACg                                                       <  1:8266234/93‑1 (MQ=255)
                                    gTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGGGAGCTATTACg                                                       >  2:2078284/1‑93 (MQ=255)
                                     tCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGa                                                      >  1:5747869/1‑93 (MQ=255)
                                     tCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGa                                                      >  2:4180694/1‑93 (MQ=255)
                                      cATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAt                                                     <  2:2748311/93‑1 (MQ=255)
                                       aTGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAtc                                                    <  1:6490332/93‑1 (MQ=255)
                                       aTGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAtc                                                    <  2:8006504/93‑1 (MQ=255)
                                         ggggCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAtctc                                                  >  1:2747876/1‑93 (MQ=255)
                                          gggCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAtctct                                                 >  2:2147040/1‑93 (MQ=255)
                                          gggCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAtctct                                                 >  2:8493898/1‑93 (MQ=255)
                                          gggCGATTCGCATTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGTGATGTACCCGGTGAGCTATTACGAtctct                                                 <  2:1950233/93‑1 (MQ=255)
                                           ggCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAtctctc                                                >  2:9937119/1‑93 (MQ=255)
                                           ggCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAtctctc                                                >  1:3298943/1‑93 (MQ=255)
                                           ggCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGAtctctc                                                >  1:528824/1‑93 (MQ=255)
                                            gCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCg                                               <  1:8517775/93‑1 (MQ=255)
                                              gATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCgca                                             <  1:5188376/93‑1 (MQ=255)
                                              gATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCgca                                             <  1:4807428/93‑1 (MQ=255)
                                                ttCGCGTTGGGACTGCACAAGAGCTTGCTACTCTCTGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGTTATTACGATCTCTCgcagg                                           <  2:5403784/93‑1 (MQ=255)
                                                  cgcgTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGCGAGGTACCCGGTGAGCTATCACCATCTCTCgcagcag                                         >  2:617458/1‑90 (MQ=255)
                                                    cgTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGcaggcagg                                       <  1:8382575/93‑1 (MQ=255)
                                                     gTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAggg                                      >  1:8204624/1‑93 (MQ=255)
                                                     gTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAggg                                      <  2:9407023/93‑1 (MQ=255)
                                                      ttGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAgggg                                     >  2:5922022/1‑93 (MQ=255)
                                                        gggACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGGGCTATTACGATCTCTCGCAGGCAGGGGTg                                   >  2:8872259/1‑93 (MQ=255)
                                                         ggACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGc                                  <  2:9186219/93‑1 (MQ=255)
                                                         ggACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGc                                  <  1:2067397/93‑1 (MQ=255)
                                                          gACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGcc                                 >  2:66009/1‑93 (MQ=255)
                                                          gACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGcc                                 <  1:8126724/93‑1 (MQ=255)
                                                           aCTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGAGGTGCCg                                >  1:4471906/1‑93 (MQ=255)
                                                            cTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCgg                               >  2:1380711/1‑93 (MQ=255)
                                                             tGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGGGCCgga                              >  1:3959222/1‑92 (MQ=255)
                                                               caccAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTAc                            <  2:1835147/93‑1 (MQ=255)
                                                                acaAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACa                           >  2:7665911/1‑93 (MQ=255)
                                                                 caAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACAt                          >  1:1473578/1‑93 (MQ=255)
                                                                 caAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACAt                          <  2:6123437/93‑1 (MQ=255)
                                                                 caAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACAt                          <  1:6422751/93‑1 (MQ=255)
                                                                  aaGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACAtt                         <  1:9953622/93‑1 (MQ=255)
                                                                  aaGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACAtt                         >  1:5962296/1‑93 (MQ=255)
                                                                  aaGAGCTTGCTACTCTTCGGCGCATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACAtt                         <  1:2437554/93‑1 (MQ=255)
                                                                   agagCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTc                        <  1:3365083/93‑1 (MQ=255)
                                                                    gagCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCg                       <  2:7875103/93‑1 (MQ=255)
                                                                       cTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACa                    <  1:1900464/93‑1 (MQ=255)
                                                                       cTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACa                    <  2:1439825/93‑1 (MQ=255)
                                                                        ttGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAg                   <  2:7746341/93‑1 (MQ=255)
                                                                         tGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGc                  <  2:1771821/93‑1 (MQ=255)
                                                                          gCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCa                 >  2:4072823/1‑93 (MQ=255)
                                                                           cTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCAt                <  1:7547386/93‑1 (MQ=255)
                                                                            tACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCAtt               <  1:6390189/93‑1 (MQ=255)
                                                                             aCTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCAttt              <  1:6075724/93‑1 (MQ=255)
                                                                             aCTCGTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCAttt              <  2:4987265/93‑1 (MQ=255)
                                                                              ctctTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTc             <  1:5530146/93‑1 (MQ=255)
                                                                              ctctTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGGGCCGGTACATTCGACAGCATTTc             >  2:6717885/1‑93 (MQ=255)
                                                                               tctTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCg            <  2:3529392/93‑1 (MQ=255)
                                                                                ctTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCgg           >  1:7173401/1‑93 (MQ=255)
                                                                                 ttCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGc          <  1:8741740/93‑1 (MQ=255)
                                                                                  tCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGcc         <  1:4702001/93‑1 (MQ=255)
                                                                                   cggcggATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATGTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGccc        >  1:9601011/1‑93 (MQ=255)
                                                                                    ggcggATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCa       >  1:4140622/1‑93 (MQ=255)
                                                                                     gcggATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGAAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCAt      <  1:6314318/93‑1 (MQ=255)
                                                                                     gaggATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCACAt      >  2:4803005/3‑93 (MQ=255)
                                                                                      cggATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCAtt     <  1:4924568/93‑1 (MQ=255)
                                                                                      cggATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCAtt     <  1:6647875/93‑1 (MQ=255)
                                                                                       ggATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATtg    >  2:822033/1‑93 (MQ=255)
                                                                                            |                                                                                         
TAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGAT  >  NC_000913/1493475‑1493656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATG  >  NC_000913/1493476‑1493660
                                                                                           |                                                                                             
AAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTT                                                                                             >  SRR4302540.6116637/1‑94 (MQ=60)
AAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTT                                                                                             >  SRR4302540.9602502/1‑94 (MQ=60)
 AAGGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTG                                                                                            <  SRR4302540.4717105/94‑1 (MQ=60)
  ATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGC                                                                                           >  SRR4302540.1175537/1‑94 (MQ=60)
   TGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCC                                                                                          <  SRR4302540.8282533/94‑1 (MQ=60)
   TGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCC                                                                                          <  SRR4302540.5478867/94‑1 (MQ=60)
   TGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCC                                                                                          <  SRR4302540.5478868/94‑1 (MQ=60)
      AGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATT                                                                                       >  SRR4302540.10042835/1‑94 (MQ=60)
       GACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTA                                                                                      <  SRR4302540.3647918/94‑1 (MQ=60)
       GACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTA                                                                                      <  SRR4302540.836934/94‑1 (MQ=60)
        GCGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTAT                                                                                     <  SRR4302540.4705603/94‑1 (MQ=60)
        ACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTAT                                                                                     <  SRR4302540.5721525/94‑1 (MQ=60)
         CGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATG                                                                                    <  SRR4302540.6245279/94‑1 (MQ=60)
         CGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATG                                                                                    >  SRR4302540.8671888/1‑94 (MQ=60)
            GCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGG                                                                                 <  SRR4302540.2617449/94‑1 (MQ=60)
              TGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGAT                                                                               >  SRR4302540.6110234/1‑94 (MQ=60)
              TGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGAT                                                                               <  SRR4302540.5318346/94‑1 (MQ=60)
              TGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGAG                                                                               >  SRR4302540.2482903/1‑94 (MQ=60)
               GGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATG                                                                              <  SRR4302540.4838493/94‑1 (MQ=60)
                GCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGT                                                                             <  SRR4302540.3631901/94‑1 (MQ=60)
                   CGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACC                                                                          <  SRR4302540.3935660/94‑1 (MQ=60)
                   CGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACC                                                                          >  SRR4302540.9999013/1‑94 (MQ=60)
                   CGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACC                                                                          >  SRR4302540.9813100/1‑94 (MQ=60)
                   CGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACC                                                                          >  SRR4302540.9812946/1‑94 (MQ=60)
                   CGACTGAATGTTGAACGTCAGGGGGCGATTCGCGTTGGGGCTGCACCATAGCTTGCGCCTCTTCGGCCGATGCTTGCCATTCTGGGCATGGACC                                                                          >  SRR4302540.9754009/1‑94 (MQ=36)
                    GACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCC                                                                         <  SRR4302540.9215828/94‑1 (MQ=60)
                     ACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCG                                                                        >  SRR4302540.86935/1‑94 (MQ=60)
                      CTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGG                                                                       <  SRR4302540.3218824/94‑1 (MQ=60)
                      CTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGG                                                                       >  SRR4302540.3645828/1‑94 (MQ=60)
                       TGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGG                                                                      >  SRR4302540.2629145/1‑94 (MQ=60)
                          ATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAG                                                                   <  SRR4302540.9615590/94‑1 (MQ=60)
                           TGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTGCTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGC                                                                  <  SRR4302540.4108599/94‑1 (MQ=60)
                           TGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGC                                                                  >  SRR4302540.8595707/1‑94 (MQ=60)
                             TTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTA                                                                <  SRR4302540.471752/94‑1 (MQ=60)
                              TGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTAT                                                               <  SRR4302540.3704304/94‑1 (MQ=60)
                                TACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTA                                                             >  SRR4302540.7659527/1‑94 (MQ=60)
                                 ACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTAC                                                            >  SRR4302540.5716287/1‑94 (MQ=60)
                                  CGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACG                                                           >  SRR4302540.239784/1‑94 (MQ=60)
                                  CGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACG                                                           <  SRR4302540.3858275/94‑1 (MQ=60)
                                  CGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACG                                                           >  SRR4302540.239785/1‑94 (MQ=60)
                                  CGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACG                                                           >  SRR4302540.239786/1‑94 (MQ=60)
                                  CGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACG                                                           <  SRR4302540.3382490/94‑1 (MQ=60)
                                   GTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGA                                                          <  SRR4302540.8362282/94‑1 (MQ=60)
                                   GTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGA                                                          >  SRR4302540.5811279/1‑94 (MQ=60)
                                     CATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATC                                                        <  SRR4302540.6711294/94‑1 (MQ=60)
                                     CATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATC                                                        <  SRR4302540.4453864/94‑1 (MQ=60)
                                      ATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCT                                                       >  SRR4302540.2833972/1‑94 (MQ=60)
                                      ATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCT                                                       <  SRR4302540.6562510/94‑1 (MQ=60)
                                       TGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTC                                                      >  SRR4302540.2777566/1‑94 (MQ=60)
                                        GGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCT                                                     <  SRR4302540.650659/94‑1 (MQ=60)
                                         GGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTC                                                    >  SRR4302540.534541/1‑94 (MQ=60)
                                         GGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTC                                                    >  SRR4302540.3335049/1‑94 (MQ=60)
                                         GGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCGC                                                    >  SRR4302540.7958245/1‑94 (MQ=60)
                                           GCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGC                                                  <  SRR4302540.8616763/94‑1 (MQ=60)
                                             GATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCTGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAG                                                <  SRR4302540.1868975/94‑1 (MQ=60)
                                             GATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAG                                                >  SRR4302540.894909/1‑94 (MQ=60)
                                             GATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAG                                                <  SRR4302540.4858882/94‑1 (MQ=60)
                                             GATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAG                                                <  SRR4302540.5244859/94‑1 (MQ=60)
                                                 CGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAG                                            <  SRR4302540.2124937/94‑1 (MQ=60)
                                                 CGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAG                                            <  SRR4302540.48288/94‑1 (MQ=60)
                                                  GCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGG                                           <  SRR4302540.3750193/94‑1 (MQ=60)
                                                   CGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGG                                          >  SRR4302540.8299967/1‑94 (MQ=60)
                                                   CGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGG                                          >  SRR4302540.3236777/1‑94 (MQ=60)
                                                   CGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGG                                          <  SRR4302540.4136341/94‑1 (MQ=60)
                                                   CGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGG                                          <  SRR4302540.8479838/94‑1 (MQ=60)
                                                   CGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGG                                          <  SRR4302540.295345/94‑1 (MQ=60)
                                                   CGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGG                                          <  SRR4302540.7204261/94‑1 (MQ=60)
                                                   CGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGCCAGGG                                          >  SRR4302540.8299973/1‑94 (MQ=60)
                                                    GTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGG                                         >  SRR4302540.5896435/1‑94 (MQ=60)
                                                      TGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTG                                       >  SRR4302540.563103/1‑94 (MQ=60)
                                                        GGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCC                                     <  SRR4302540.2089636/94‑1 (MQ=60)
                                                         GACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCG                                    <  SRR4302540.8221052/94‑1 (MQ=60)
                                                         GACTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGAGGTGCCG                                    >  SRR4302540.4520406/1‑94 (MQ=60)
                                                           CTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGGGCCGGA                                  >  SRR4302540.8636147/1‑94 (MQ=60)
                                                           CTGCACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGGGCCGGA                                  >  SRR4302540.4001929/1‑94 (MQ=60)
                                                               ACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACAT                              >  SRR4302540.8603083/1‑94 (MQ=60)
                                                               ACAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACAT                              >  SRR4302540.1489689/1‑94 (MQ=60)
                                                                CAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATT                             >  SRR4302540.6028259/1‑94 (MQ=60)
                                                                CAAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATT                             <  SRR4302540.6494239/94‑1 (MQ=60)
                                                                 AAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTC                            <  SRR4302540.10069812/94‑1 (MQ=60)
                                                                 AAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTC                            <  SRR4302540.4949912/94‑1 (MQ=60)
                                                                 AAGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATAACATTCTCTCGCAGGACGGGGGGCCGGTACATTC                            >  SRR4302540.8521073/1‑94 (MQ=60)
                                                                 AAGAGCTTGCTACTCTTCGGCGCATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTC                            <  SRR4302540.2463803/94‑1 (MQ=60)
                                                                  AGAGCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCG                           <  SRR4302540.3401898/94‑1 (MQ=60)
                                                                     GCTTGCTACTCTTCGGCGGATGCTTGCCTTTATAGGGTTGTACCCGGTGAGCTATTACAATCTCTTGCTGGCAGGGGCGCCGGTACTTTCGATA                        >  SRR4302540.6530022/1‑94 (MQ=48)
                                                                     GCTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACA                        <  SRR4302540.8592599/94‑1 (MQ=60)
                                                                      CTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAG                       <  SRR4302540.1921248/94‑1 (MQ=60)
                                                                      CTTGCTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAG                       <  SRR4302540.3998535/94‑1 (MQ=60)
                                                                          CTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATT                   <  SRR4302540.6410361/94‑1 (MQ=60)
                                                                          CTACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATT                   <  SRR4302540.7631975/94‑1 (MQ=60)
                                                                           TACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTT                  <  SRR4302540.6461324/94‑1 (MQ=60)
                                                                           TACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTT                  <  SRR4302540.6461325/94‑1 (MQ=60)
                                                                            ACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTC                 <  SRR4302540.6143284/94‑1 (MQ=60)
                                                                            ACTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTC                 <  SRR4302540.9311256/94‑1 (MQ=60)
                                                                             CTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCG                <  SRR4302540.5591050/94‑1 (MQ=60)
                                                                             CTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCG                >  SRR4302540.9070851/1‑94 (MQ=60)
                                                                             CTCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCG                >  SRR4302540.5189935/1‑94 (MQ=60)
                                                                              TCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTTGG               >  SRR4302540.3905707/1‑94 (MQ=60)
                                                                              TCTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGG               >  SRR4302540.7253356/1‑94 (MQ=60)
                                                                               CTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGC              >  SRR4302540.9907745/1‑94 (MQ=60)
                                                                               CTTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGC              >  SRR4302540.3444563/1‑94 (MQ=60)
                                                                                TTCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCC             <  SRR4302540.8843214/94‑1 (MQ=60)
                                                                                 TCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATGTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCC            >  SRR4302540.9714271/1‑94 (MQ=60)
                                                                                 TCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCC            <  SRR4302540.4752250/94‑1 (MQ=60)
                                                                                 TCGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCC            >  SRR4302540.9905537/1‑94 (MQ=60)
                                                                                  CGGCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCA           >  SRR4302540.4185424/1‑94 (MQ=60)
                                                                                    GCGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGAAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATT         <  SRR4302540.6384636/94‑1 (MQ=60)
                                                                                     CGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTG        <  SRR4302540.6721417/94‑1 (MQ=60)
                                                                                     CGGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTG        <  SRR4302540.4977641/94‑1 (MQ=60)
                                                                                      GGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGA       >  SRR4302540.2039154/1‑94 (MQ=60)
                                                                                      GGATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGA       >  SRR4302540.7280600/1‑94 (MQ=60)
                                                                                       GATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGAT      <  SRR4302540.1290727/94‑1 (MQ=60)
                                                                                        ATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATG     >  SRR4302540.3744462/1‑94 (MQ=60)
                                                                                        ATGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATG     >  SRR4302540.8768149/1‑94 (MQ=60)
                                                                                         TGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCGCATTGATGA    >  SRR4302540.1274878/1‑94 (MQ=60)
                                                                                         TGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGA    >  SRR4302540.1274879/1‑94 (MQ=60)
                                                                                         TGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGA    <  SRR4302540.8290104/94‑1 (MQ=60)
                                                                                         TGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTAC‑‑TCGACAGCATTTCGGCCCATTGATGATG  >  SRR4302540.4139327/1‑94 (MQ=60)
                                                                                         TGCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTAC‑‑TCGACAGCATTTCGGCCCATTGATGATG  >  SRR4302540.4139325/1‑94 (MQ=60)
                                                                                          GCTTGCCATTATGGGGATGTTCCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGAT   <  SRR4302540.2659686/94‑1 (MQ=60)
                                                                                          GCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGAT   >  SRR4302540.10070571/1‑94 (MQ=60)
                                                                                          GCTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGAT   <  SRR4302540.6014290/94‑1 (MQ=60)
                                                                                           CTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATG  >  SRR4302540.2183921/1‑94 (MQ=60)
                                                                                           |                                                                                             
AAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCGCAGGCAGGGGTGCCGGTACATTCGACAGCATTTCGGCCCATTGATGATG  >  NC_000913/1493476‑1493660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑