Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,503,660 T→C T149A (ACG→GCG)  b1433 ← putative membrane transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,503,6600TC90.9% 606.3 / 42.4 230T149A (ACG→GCG) b1433putative membrane transport protein
Reads supporting (aligned to +/- strand):  ref base T (9/12);  new base C (108/101);  total (117/113)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 234 total aligned reads displayed.

ACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGC  >  NC_000913/1503569‑1503752
                                                                                           |                                                                                            
acgatcCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTa                                                                                             <  1:7864314/93‑1 (MQ=255)
 cgatcCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCaa                                                                                            >  1:6639304/1‑93 (MQ=255)
   atcCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAAt                                                                                          >  1:846058/1‑93 (MQ=255)
   atcCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAAt                                                                                          >  1:5659208/1‑93 (MQ=255)
    tcCCAATAATCATCGCGGCAATTACCACATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAAtt                                                                                         >  2:40558/1‑93 (MQ=255)
      ccAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGa                                                                                       >  1:2234722/1‑93 (MQ=255)
       cAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGAc                                                                                      <  2:7635246/93‑1 (MQ=255)
       cAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGAc                                                                                      <  2:6181323/93‑1 (MQ=255)
       cAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGAc                                                                                      >  1:177966/1‑93 (MQ=255)
        aataatCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGAcc                                                                                     >  2:8852056/1‑93 (MQ=255)
        aataatCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGAcc                                                                                     >  1:306249/1‑93 (MQ=255)
         ataatCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCg                                                                                    >  1:5476931/1‑93 (MQ=255)
          taatCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGt                                                                                   >  2:5706213/1‑93 (MQ=255)
           aatCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTc                                                                                  >  2:8326842/1‑93 (MQ=255)
           aatCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTc                                                                                  <  1:2764806/93‑1 (MQ=255)
             tcatcGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCa                                                                                <  1:9101666/93‑1 (MQ=255)
                tcGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGAc                                                                             >  2:8210356/1‑93 (MQ=255)
                tcGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGAc                                                                             <  2:807774/93‑1 (MQ=255)
                tcGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGAc                                                                             >  2:8809031/1‑93 (MQ=255)
                 cgcgGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACt                                                                            <  2:2052809/93‑1 (MQ=255)
                  gcgGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTg                                                                           >  1:5711405/1‑93 (MQ=255)
                   cgGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTgg                                                                          >  1:1277551/1‑93 (MQ=255)
                    ggCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGc                                                                         <  1:902433/93‑1 (MQ=255)
                    ggCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGc                                                                         <  1:1659107/93‑1 (MQ=255)
                    ggCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGc                                                                         <  2:8496681/93‑1 (MQ=255)
                        aTTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAAc                                                                     >  2:7272244/1‑93 (MQ=255)
                        aTTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAAc                                                                     >  2:2626325/1‑93 (MQ=255)
                        aTTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAAc                                                                     >  2:9601765/1‑93 (MQ=255)
                        aTTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAAc                                                                     >  1:4969037/1‑93 (MQ=255)
                         ttACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACg                                                                    >  2:2107456/1‑93 (MQ=255)
                          tACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGc                                                                   <  1:3735689/93‑1 (MQ=255)
                           aCCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGcc                                                                  >  1:1541633/1‑93 (MQ=255)
                             cGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTg                                                                <  2:7567156/93‑1 (MQ=255)
                                aTAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTaa                                                             >  1:3617576/1‑93 (MQ=255)
                                aTAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTaa                                                             >  2:2872148/1‑93 (MQ=255)
                                 tAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTaaa                                                            <  2:1786179/93‑1 (MQ=255)
                                  aggccggcgCAACGGCCTTGGTTGCCAGCCATTCCAGCAACATCCTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAAc                                                           <  1:1751938/89‑1 (MQ=255)
                                  aGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAAc                                                           >  2:6682675/1‑93 (MQ=255)
                                  aGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAAc                                                           <  1:9997245/93‑1 (MQ=255)
                                   gcgcGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAAcc                                                          <  1:8913864/93‑1 (MQ=255)
                                   gcgcGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAAcc                                                          >  2:3271519/1‑93 (MQ=255)
                                      gggcgCAAGGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCaaa                                                       <  1:2895291/92‑1 (MQ=255)
                                       ggcgCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAg                                                      <  1:607571/93‑1 (MQ=255)
                                        gcgcAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGc                                                     >  1:3546747/1‑93 (MQ=255)
                                        gcgcAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGc                                                     >  1:9724146/1‑93 (MQ=255)
                                         cgcAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCg                                                    >  2:3512311/1‑93 (MQ=255)
                                          gcAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGt                                                   <  2:2896607/93‑1 (MQ=255)
                                             aCGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTCAACCAAAGCGtaat                                                >  1:7031387/1‑93 (MQ=255)
                                             aCGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGtaat                                                >  1:1428317/1‑93 (MQ=255)
                                              cGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGtaata                                               <  2:6778423/93‑1 (MQ=255)
                                                gCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATaaa                                             <  2:3870105/93‑1 (MQ=255)
                                                gCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATaaa                                             >  1:6416750/1‑93 (MQ=255)
                                                 ccTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATaaaa                                            <  2:1486632/93‑1 (MQ=255)
                                                   ttGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATc                                          >  1:8314953/1‑93 (MQ=255)
                                                   ttGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATc                                          >  2:7607648/1‑93 (MQ=255)
                                                   ttGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATc                                          >  1:5939009/1‑93 (MQ=255)
                                                    tGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATcc                                         <  1:9472148/93‑1 (MQ=255)
                                                     ggTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATccc                                        <  2:6345904/93‑1 (MQ=255)
                                                     ggTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATccc                                        <  2:9079443/93‑1 (MQ=255)
                                                      gTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCg                                       >  2:8636399/1‑93 (MQ=255)
                                                           cagccaTACCAGCAACGTCCTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAg                                  <  1:9869321/93‑1 (MQ=255)
                                                             gccaTACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCa                                >  2:440958/1‑93 (MQ=255)
                                                             gccaTACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCa                                >  1:7431908/1‑93 (MQ=255)
                                                               caTACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCAtt                              >  2:9836672/1‑93 (MQ=255)
                                                               caTACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCAtt                              >  1:8782860/1‑93 (MQ=255)
                                                                atcccAGCAACATACTTCCACACAACGCAAATTGACCGTCGAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTg                             <  1:1759011/90‑1 (MQ=255)
                                                                 tACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTgc                            <  2:8779810/93‑1 (MQ=255)
                                                                   ccAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTgccg                          >  2:51974/1‑93 (MQ=255)
                                                                    cAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTgccgc                         <  1:6514339/93‑1 (MQ=255)
                                                                    cAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTgccgc                         >  2:7978016/1‑93 (MQ=255)
                                                                    cAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTgccgc                         <  1:4802146/93‑1 (MQ=255)
                                                                      gCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGccgccg                       >  1:7260268/1‑93 (MQ=255)
                                                                        aaCATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCa                     >  2:6057663/1‑93 (MQ=255)
                                                                        aaCATACTTCCACACAACGCAAATTGACCGTCCAGACTGACAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCCCa                     >  2:6098228/1‑93 (MQ=255)
                                                                        aaCATACTTACAGACAACGCAAATTGGCAGTCCAGGCTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCa                     <  1:6101774/93‑1 (MQ=255)
                                                                         aCATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCaa                    >  1:5122251/1‑93 (MQ=255)
                                                                          cATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAg                   <  1:1236199/93‑1 (MQ=255)
                                                                          cATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAg                   >  1:5518073/1‑93 (MQ=255)
                                                                          cATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAg                   >  2:5063972/1‑93 (MQ=255)
                                                                          cATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAg                   <  1:4309014/93‑1 (MQ=255)
                                                                           aTACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGc                  <  2:6563856/93‑1 (MQ=255)
                                                                            tACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCg                 <  1:6121376/93‑1 (MQ=255)
                                                                             aCTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGa                <  1:1152138/93‑1 (MQ=255)
                                                                              cTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAg               <  2:581273/93‑1 (MQ=255)
                                                                              cTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAg               <  2:140327/93‑1 (MQ=255)
                                                                               ttCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAgg              >  1:9296211/1‑92 (MQ=255)
                                                                               ttCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGt              <  2:5413551/93‑1 (MQ=255)
                                                                               ttCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGt              >  1:4811459/1‑93 (MQ=255)
                                                                                tCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTg             <  1:2431682/93‑1 (MQ=255)
                                                                                tCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTg             <  1:4669189/93‑1 (MQ=255)
                                                                                 gcacacaACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAGCCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGc            <  2:1317270/92‑1 (MQ=255)
                                                                                 ccACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGc            >  1:7738683/1‑93 (MQ=255)
                                                                                  cacacaACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCg           <  1:4625940/93‑1 (MQ=255)
                                                                                  cacacaACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCg           >  2:9968825/1‑93 (MQ=255)
                                                                                   acacaACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCgg          >  2:4984232/1‑93 (MQ=255)
                                                                                     acaACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGaa        >  1:2159402/1‑93 (MQ=255)
                                                                                      caACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGaat       >  2:4504510/1‑93 (MQ=255)
                                                                                        aCGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGaataa     >  2:400242/1‑93 (MQ=255)
                                                                                        aCGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGGGCGGaataa     >  2:547711/1‑93 (MQ=255)
                                                                                           tAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATgc  <  1:5389839/93‑1 (MQ=255)
                                                                                           |                                                                                            
ACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGC  >  NC_000913/1503569‑1503752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCG  >  NC_000913/1503567‑1503753
                                                                                             |                                                                                             
TCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGT                                                                                               <  SRR4302540.60733/94‑1 (MQ=60)
  ACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAA                                                                                             <  SRR4302540.7956192/94‑1 (MQ=60)
  ACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAA                                                                                             >  SRR4302540.6712774/1‑94 (MQ=60)
   CGATCCCAATAATAATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAA                                                                                            >  SRR4302540.9601700/1‑94 (MQ=60)
    GATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAAT                                                                                           <  SRR4302540.216739/94‑1 (MQ=60)
    GATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAAT                                                                                           >  SRR4302540.855033/1‑94 (MQ=60)
    GATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAAT                                                                                           >  SRR4302540.905432/1‑94 (MQ=60)
    GATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAAT                                                                                           >  SRR4302540.5721522/1‑94 (MQ=60)
    GATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAAT                                                                                           <  SRR4302540.9592257/94‑1 (MQ=60)
      TCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTG                                                                                         <  SRR4302540.5580361/94‑1 (MQ=60)
      TCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTG                                                                                         >  SRR4302540.2971037/1‑94 (MQ=60)
       CCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGA                                                                                        >  SRR4302540.2258768/1‑94 (MQ=60)
        CCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGAC                                                                                       <  SRR4302540.5265939/94‑1 (MQ=60)
        CCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGAC                                                                                       >  SRR4302540.179731/1‑94 (MQ=60)
         CAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACC                                                                                      >  SRR4302540.309291/1‑94 (MQ=60)
         CAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACC                                                                                      >  SRR4302540.9291526/1‑94 (MQ=60)
          AATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCG                                                                                     >  SRR4302540.5537218/1‑94 (MQ=60)
             AATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCC                                                                                  <  SRR4302540.2794680/94‑1 (MQ=60)
               TCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAG                                                                                >  SRR4302540.1547081/1‑94 (MQ=60)
               TCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAG                                                                                <  SRR4302540.9211036/94‑1 (MQ=60)
                CATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGA                                                                               >  SRR4302540.510097/1‑94 (MQ=60)
                 ATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGAC                                                                              <  SRR4302540.3730491/94‑1 (MQ=60)
                 ATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGAC                                                                              <  SRR4302540.6231488/94‑1 (MQ=60)
                  TCGCGAGAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATTCCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACT                                                                             <  SRR4302540.1704052/94‑1 (MQ=60)
                   CGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTG                                                                            >  SRR4302540.5774275/1‑94 (MQ=60)
                    GCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGG                                                                           >  SRR4302540.1290720/1‑94 (MQ=60)
                     CGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGC                                                                          <  SRR4302540.740553/94‑1 (MQ=60)
                      GGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCA                                                                         <  SRR4302540.1677185/94‑1 (MQ=60)
                      GGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCA                                                                         <  SRR4302540.1677183/94‑1 (MQ=60)
                      GGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCA                                                                         <  SRR4302540.6674504/94‑1 (MQ=60)
                      GGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCA                                                                         >  SRR4302540.7465433/1‑94 (MQ=60)
                      GGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCA                                                                         <  SRR4302540.912015/94‑1 (MQ=60)
                      GGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCA                                                                         <  SRR4302540.6674501/94‑1 (MQ=60)
                       GCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAA                                                                        <  SRR4302540.9499437/94‑1 (MQ=60)
                         AATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAAC                                                                      >  SRR4302540.5022801/1‑94 (MQ=60)
                         AATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAAC                                                                      <  SRR4302540.4696795/94‑1 (MQ=60)
                         AATGACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAAC                                                                      <  SRR4302540.4126849/94‑1 (MQ=60)
                            TACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCC                                                                   >  SRR4302540.1558665/1‑94 (MQ=60)
                            TACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCC                                                                   <  SRR4302540.3776447/94‑1 (MQ=60)
                              CCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCGAATTGACCGTCCAGACTGGCAAACGCCTG                                                                 >  SRR4302540.3990557/1‑94 (MQ=60)
                               CGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACATCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGT                                                                <  SRR4302540.6184332/94‑1 (MQ=60)
                                GCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTA                                                               >  SRR4302540.8407555/1‑94 (MQ=60)
                                 CATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAA                                                              >  SRR4302540.3657147/1‑94 (MQ=60)
                                 CATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAA                                                              >  SRR4302540.4828154/1‑94 (MQ=60)
                                    aggcCGGCGCAACGGCCTTGGTTGCCAGCCATTCCAGCAACATCCTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACC                                                           <  SRR4302540.1770918/90‑1 (MQ=60)
                                    AGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACC                                                           <  SRR4302540.1770919/94‑1 (MQ=60)
                                    AGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACC                                                           <  SRR4302540.10113730/94‑1 (MQ=60)
                                    AGCGCGGCGCAACGGCCTTAGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACC                                                           <  SRR4302540.9894333/94‑1 (MQ=60)
                                     GCGCGGCGCAACGGCCTTGTTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCA                                                          <  SRR4302540.9017091/94‑1 (MQ=60)
                                     GCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCA                                                          <  SRR4302540.9017092/94‑1 (MQ=60)
                                      CGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAA                                                         >  SRR4302540.2827738/1‑94 (MQ=60)
                                        GGGCGCAAGGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAG                                                       <  SRR4302540.2926866/94‑1 (MQ=60)
                                         GGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGC                                                      >  SRR4302540.3585488/1‑94 (MQ=60)
                                         GGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGC                                                      <  SRR4302540.614068/94‑1 (MQ=60)
                                         GGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGC                                                      >  SRR4302540.9838363/1‑94 (MQ=60)
                                             CAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAA                                                  <  SRR4302540.4169516/94‑1 (MQ=60)
                                              AACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTCAACCAAAGCGTAAT                                                 >  SRR4302540.7109528/1‑94 (MQ=60)
                                              AACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAAT                                                 >  SRR4302540.1443779/1‑94 (MQ=60)
                                               ACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATA                                                <  SRR4302540.7626739/94‑1 (MQ=60)
                                               ACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATA                                                <  SRR4302540.117666/94‑1 (MQ=60)
                                                CGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAA                                               <  SRR4302540.9250168/94‑1 (MQ=60)
                                                CGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAA                                               >  SRR4302540.2135064/1‑94 (MQ=60)
                                                 GGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAA                                              >  SRR4302540.5529344/1‑94 (MQ=60)
                                                 GGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAA                                              >  SRR4302540.6488165/1‑94 (MQ=60)
                                                 GGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAA                                              >  SRR4302540.8885291/1‑94 (MQ=60)
                                                 GGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAA                                              >  SRR4302540.359497/1‑94 (MQ=60)
                                                 GGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAA                                              >  SRR4302540.7974506/1‑94 (MQ=60)
                                                  GCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAA                                             >  SRR4302540.4388151/1‑94 (MQ=60)
                                                   CTTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAAT                                            <  SRR4302540.7199884/94‑1 (MQ=60)
                                                   CCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAAT                                            >  SRR4302540.2727981/1‑94 (MQ=60)
                                                    CTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATC                                           >  SRR4302540.8411474/1‑94 (MQ=60)
                                                    CTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATC                                           >  SRR4302540.6004659/1‑94 (MQ=60)
                                                     TTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCC                                          >  SRR4302540.2471488/1‑94 (MQ=60)
                                                      TGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCC                                         <  SRR4302540.9584496/94‑1 (MQ=60)
                                                            CCAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAG                                   <  SRR4302540.4735641/94‑1 (MQ=60)
                                                             CAGCCATACCAGCAACGTCCTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGC                                  <  SRR4302540.9984588/94‑1 (MQ=60)
                                                             CAGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGC                                  <  SRR4302540.6068792/94‑1 (MQ=60)
                                                              AGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCA                                 >  SRR4302540.7514701/1‑94 (MQ=60)
                                                              AGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCA                                 >  SRR4302540.5560435/1‑94 (MQ=60)
                                                              AGCCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCA                                 >  SRR4302540.5560147/1‑94 (MQ=60)
                                                                CCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATT                               <  SRR4302540.2229212/94‑1 (MQ=60)
                                                                CCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATT                               <  SRR4302540.5733179/94‑1 (MQ=60)
                                                                CCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATT                               >  SRR4302540.8884529/1‑94 (MQ=60)
                                                                CCATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATT                               >  SRR4302540.695235/1‑94 (MQ=60)
                                                                  ATCCCAGCAACATACTTCCACACAACGCAAATTGACCGTCGAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGC                             <  SRR4302540.1778081/94‑1 (MQ=60)
                                                                  ATACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGC                             <  SRR4302540.1778082/94‑1 (MQ=60)
                                                                    ACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCG                           >  SRR4302540.9194403/1‑94 (MQ=60)
                                                                    ACCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCG                           <  SRR4302540.5057221/94‑1 (MQ=60)
                                                                     CCAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGC                          >  SRR4302540.1278508/1‑94 (MQ=60)
                                                                      CAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCC                         <  SRR4302540.6586708/94‑1 (MQ=60)
                                                                      CAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCC                         >  SRR4302540.1394852/1‑94 (MQ=60)
                                                                      CAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCC                         >  SRR4302540.1394518/1‑94 (MQ=60)
                                                                      CAGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCC                         <  SRR4302540.4853540/94‑1 (MQ=60)
                                                                       AGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCG                        >  SRR4302540.3148118/1‑94 (MQ=60)
                                                                       AGCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCG                        >  SRR4302540.7341281/1‑94 (MQ=60)
                                                                        GCAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGC                       >  SRR4302540.5917545/1‑94 (MQ=60)
                                                                         CAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCA                      >  SRR4302540.3197695/1‑94 (MQ=60)
                                                                         CAACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCA                      <  SRR4302540.10073093/94‑1 (MQ=60)
                                                                          AACATACTTCCACCCAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAA                     <  SRR4302540.7882509/94‑1 (MQ=60)
                                                                          AACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAA                     <  SRR4302540.6169710/94‑1 (MQ=60)
                                                                          AACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAA                     >  SRR4302540.5177710/1‑94 (MQ=60)
                                                                          AACATACTTACAGACAACGCAAATTGGCAGTCCAGGCTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAA                     <  SRR4302540.6169711/94‑1 (MQ=60)
                                                                           ACATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAG                    >  SRR4302540.5578854/1‑94 (MQ=60)
                                                                            CATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGC                   <  SRR4302540.1248821/94‑1 (MQ=60)
                                                                            CATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGC                   <  SRR4302540.6558985/94‑1 (MQ=60)
                                                                            CATACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGC                   <  SRR4302540.4355919/94‑1 (MQ=60)
                                                                              TACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGA                 <  SRR4302540.6189554/94‑1 (MQ=60)
                                                                              TACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAAGCCCGGCAAGCATTGCCGCCGCAAGCGA                 <  SRR4302540.6189552/94‑1 (MQ=60)
                                                                               ACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAG                <  SRR4302540.1164140/94‑1 (MQ=60)
                                                                               ACTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAG                <  SRR4302540.8771793/94‑1 (MQ=60)
                                                                                CTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGT               <  SRR4302540.4979816/94‑1 (MQ=60)
                                                                                CTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGT               >  SRR4302540.4862958/1‑94 (MQ=60)
                                                                                CTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGG               >  SRR4302540.9407276/1‑94 (MQ=60)
                                                                                CTTCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGG               >  SRR4302540.4862956/1‑94 (MQ=60)
                                                                                  TCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGC             <  SRR4302540.4719166/94‑1 (MQ=60)
                                                                                  TCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGC             <  SRR4302540.2457880/94‑1 (MQ=60)
                                                                                  TCCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGC             >  SRR4302540.7829026/1‑94 (MQ=60)
                                                                                   CCACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCG            <  SRR4302540.160100/94‑1 (MQ=60)
                                                                                    CACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGG           <  SRR4302540.4675504/94‑1 (MQ=60)
                                                                                    CACACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGG           >  SRR4302540.5963112/1‑94 (MQ=60)
                                                                                      CACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAA         >  SRR4302540.2182656/1‑94 (MQ=60)
                                                                                       ACAACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAAT        >  SRR4302540.8453716/1‑94 (MQ=60)
                                                                                          ACGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAAT     <  SRR4302540.2339236/94‑1 (MQ=60)
                                                                                           CGCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATG    >  SRR4302540.451690/1‑94 (MQ=60)
                                                                                            GCAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGC   >  SRR4302540.2685061/1‑94 (MQ=60)
                                                                                             TAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCG  <  SRR4302540.5449112/94‑1 (MQ=60)
                                                                                             |                                                                                             
TCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTGGCAAACGCCTGTAAACCAAAGCGTAATAAAATCCCGGCAAGCATTGCCGCCGCAAGCGAGTGCGGAATAATGCG  >  NC_000913/1503567‑1503753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑