Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,531,361	T→C	H188R (CAT→CGT)	b1462 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,531,361	0	T	C	100.0%	598.0 / NA	183	H188R (CAT→CGT)	b1462	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (105/78); total (105/78)

Only 100 of 192 total aligned reads displayed.

GCTTTGATAAAGAAACC‑TGGTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAAC  >  NC_000913/1531271‑1531450

gCTTTGATAAAGAAACC‑TGGTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGc                                                                                         >  1:222818/1‑93 (MQ=255)

    tGATAAAGAAACC‑TGGTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACg                                                                                     >  1:5754767/1‑93 (MQ=255)

     taaaaaaaaACCTTGTTTAAAGCATAAATGCTACC‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACg                                                                                     <  1:4471/90‑1 (MQ=255)

       tAAAGAAACC‑TGGTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTAt                                                                                  >  1:6001061/1‑93 (MQ=255)

       tAAAGAAACC‑TGGTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTAt                                                                                  >  2:8381926/1‑93 (MQ=255)

       aaaaaaaACCTTGGTTATAGAAGAATTCCTCAC‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTa                                                                                   <  1:3898974/92‑1 (MQ=255)

        aaaaaaACCTTGGTTATAGCATGATTCCTTAC‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTAt                                                                                  <  2:4507880/93‑1 (MQ=255)

        aaaGAACCG‑TGGTTATAGAATGAATCCTAAC‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTAtt                                                                                 <  1:1118878/93‑1 (MQ=255)

              aCC‑TGGTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCtt                                                                           >  2:3228147/1‑93 (MQ=255)

              aCC‑TGGTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCtt                                                                           >  1:5889868/1‑93 (MQ=255)

              aCC‑TGGTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCtt                                                                           >  1:7196275/1‑93 (MQ=255)

                 ttgtttATAGAAGGAATGCTCAC‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTat                                                                         <  1:3843893/89‑1 (MQ=255)

                  ttcttATAGTATAAGTGCATAC‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTatc                                                                        <  1:4648088/90‑1 (MQ=255)

                   ggTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTatca                                                                       >  2:5502742/1‑93 (MQ=255)

                   ggTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTatca                                                                       >  1:5155364/1‑93 (MQ=255)

                    gTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTatcat                                                                      >  1:7936798/1‑93 (MQ=255)

                    gTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTatcat                                                                      >  1:2485749/1‑93 (MQ=255)

                     ttATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTatcatc                                                                     >  1:3444818/1‑93 (MQ=255)

                      tataGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTatcatca                                                                    >  2:673151/1‑93 (MQ=255)

                         aGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTAtcatcatca                                                                 >  1:4989532/1‑93 (MQ=255)

                            aTGAATGCTTCC‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAAc                                                              <  1:6892839/93‑1 (MQ=255)

                            aTGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAAc                                                              >  1:3596348/1‑93 (MQ=255)

                            aTGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAAc                                                              >  1:5360971/1‑93 (MQ=255)

                              gAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTg                                                            >  1:7326775/1‑93 (MQ=255)

                              gAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTg                                                            >  1:146862/1‑93 (MQ=255)

                              gAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTg                                                            >  2:4136180/1‑93 (MQ=255)

                               aaTGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGGAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGc                                                           >  1:9893793/1‑93 (MQ=255)

                                  tcTTAACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCa                                                         <  2:3223062/92‑1 (MQ=255)

                                  gCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAg                                                        >  1:9011931/1‑93 (MQ=255)

                                    ttAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCg                                                      >  2:770765/1‑93 (MQ=255)

                                     ttcgcccGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCg                                                      <  2:4582643/89‑1 (MQ=255)

                                     tcaccccGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCg                                                      <  2:1593469/89‑1 (MQ=255)

                                     cccccccGCCGTAACACGCTTGCCGCTGATAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCg                                                      <  2:1905192/89‑1 (MQ=255)

                                      aa‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGc                                                    >  1:6050861/1‑93 (MQ=255)

                                      aa‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGc                                                    >  2:3469486/1‑93 (MQ=255)

                                         cccGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCt                                                  <  2:8802494/93‑1 (MQ=255)

                                         cccGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCt                                                  <  1:9373952/93‑1 (MQ=255)

                                          ccgccgTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCtt                                                 <  2:2714717/93‑1 (MQ=255)

                                           cgccgTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTc                                                >  1:7930520/1‑93 (MQ=255)

                                             ccgTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACGTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGa                                              >  1:856862/1‑93 (MQ=255)

                                             ccgTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCATCGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGa                                              <  1:427338/93‑1 (MQ=255)

                                             ccgTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGa                                              >  2:9844161/1‑93 (MQ=255)

                                             ccgTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGa                                              >  2:5244852/1‑93 (MQ=255)

                                              cgTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGAc                                             <  2:6028696/93‑1 (MQ=255)

                                              cgTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGAc                                             >  1:6984086/1‑93 (MQ=255)

                                              cgTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGAc                                             <  1:809031/93‑1 (MQ=255)

                                               gTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACa                                            <  1:8916385/93‑1 (MQ=255)

                                               gTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACa                                            >  1:3218995/1‑93 (MQ=255)

                                               gTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACa                                            <  2:2091532/93‑1 (MQ=255)

                                                tAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTcaacaa                                           >  2:6527917/1‑93 (MQ=255)

                                                tAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACaa                                           >  1:8138943/1‑93 (MQ=255)

                                                   cacTCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATa                                        <  1:9581640/93‑1 (MQ=255)

                                                   cacGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATa                                        <  2:5902095/93‑1 (MQ=255)

                                                   cacGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATa                                        >  1:9299592/1‑93 (MQ=255)

                                                   cacGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATa                                        <  1:4406843/93‑1 (MQ=255)

                                                    acGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAc                                       >  2:6082272/1‑93 (MQ=255)

                                                    acGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAc                                       >  1:8746576/1‑93 (MQ=255)

                                                     cGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAcc                                      <  1:6195983/93‑1 (MQ=255)

                                                      gCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAccc                                     >  1:1285458/1‑93 (MQ=255)

                                                      gCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAccc                                     >  2:6725076/1‑93 (MQ=255)

                                                       cTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCg                                    >  2:917208/1‑93 (MQ=255)

                                                       cTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCg                                    <  2:32929/93‑1 (MQ=255)

                                                        ttGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGt                                   <  1:9576808/93‑1 (MQ=255)

                                                          gCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGc                                 <  1:3463187/93‑1 (MQ=255)

                                                            cGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGt                               >  2:8638092/1‑93 (MQ=255)

                                                             gCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTc                              >  2:4328813/1‑93 (MQ=255)

                                                              cTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCt                             <  1:1720800/93‑1 (MQ=255)

                                                              cTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCt                             <  2:4925207/93‑1 (MQ=255)

                                                              cTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCt                             <  2:5311413/93‑1 (MQ=255)

                                                               tGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACACATACCCGTGCGTCTg                            >  1:7389415/1‑93 (MQ=255)

                                                                ggTAACAAACGTCCCAGCACCTAGATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGc                           <  2:6941248/93‑1 (MQ=255)

                                                                  tAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCg                         >  1:8503660/1‑93 (MQ=255)

                                                                   aaCAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCgc                        >  1:4832219/1‑93 (MQ=255)

                                                                   aaCAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCgc                        <  2:5330372/93‑1 (MQ=255)

                                                                   aaCAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCgc                        <  2:4888980/93‑1 (MQ=255)

                                                                      aaaCGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCgctgc                     <  1:2918004/93‑1 (MQ=255)

                                                                      aaaCGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCgctgc                     <  2:5447986/93‑1 (MQ=255)

                                                                      aaaCGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCgctgc                     >  2:7428954/1‑93 (MQ=255)

                                                                       aaCGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCgctgct                    >  2:1686605/1‑93 (MQ=255)

                                                                        aCGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGctgctg                   <  2:90300/93‑1 (MQ=255)

                                                                          gTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAt                 <  2:9345529/93‑1 (MQ=255)

                                                                           tCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAta                >  1:1126898/1‑93 (MQ=255)

                                                                            cccAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAtac               <  1:9226459/93‑1 (MQ=255)

                                                                            cccAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAtac               >  2:1144065/1‑93 (MQ=255)

                                                                             ccAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAtact              <  2:4124069/93‑1 (MQ=255)

                                                                             ccAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTG‑CAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAtacta             >  1:7531022/1‑93 (MQ=255)

                                                                              cAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAtacta             >  2:8186917/1‑93 (MQ=255)

                                                                               aGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAtactac            <  1:5729142/93‑1 (MQ=255)

                                                                               aGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAtactac            >  2:2324205/1‑93 (MQ=255)

                                                                               aGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGAtactac            >  1:2047151/1‑93 (MQ=255)

                                                                                gCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATactact           >  2:9566286/1‑93 (MQ=255)

                                                                                 cACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACtt          <  2:2368423/93‑1 (MQ=255)

                                                                                   ccTAAATGACGCAACGTATTGAGCTTATCATCATCAACCTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCg        <  2:2982250/93‑1 (MQ=255)

                                                                                   ccTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCg        >  2:3982987/1‑93 (MQ=255)

                                                                                     tAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGcc      <  1:2719024/93‑1 (MQ=255)

                                                                                     tAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGcc      >  2:6158233/1‑93 (MQ=255)

                                                                                       aaTGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCaa    <  2:6979181/93‑1 (MQ=255)

                                                                                         tgacgCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAAc  >  2:3480018/1‑93 (MQ=255)

                                                                                         tgacgCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAAc  <  1:1167196/93‑1 (MQ=255)

                                                                                         tgacgCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAAc  <  1:9121239/93‑1 (MQ=255)

GCTTTGATAAAGAAACC‑TGGTTATAGCATGAATGCTTAA‑CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAAC  >  NC_000913/1531271‑1531450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAG  >  NC_000913/1531268‑1531452

GAGGTTTTGAAAAAAAACCCTGTTTAAAGCATGAATGCTTACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGAC                                                                                             <  SRR4302540.8684079/94‑1 (MQ=60)

GAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGAC                                                                                             >  SRR4302540.6214866/1‑94 (MQ=60)

GAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGAC                                                                                             >  SRR4302540.6214868/1‑94 (MQ=60)

 gtgtttaaaaaaagaaccttgttaaaaagataacttcttacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACG                                                                                            <  SRR4302540.2108741/53‑1 (MQ=60)

 ggtttttaaaaaagaacccgGGTTAGAGCATGAATGCTTACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACG                                                                                            <  SRR4302540.4166662/74‑1 (MQ=60)

  GGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGC                                                                                           >  SRR4302540.224976/1‑94 (MQ=60)

   tcttaaaaaaaaaaccctgtTTATAGCATAAATGCTAACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCA                                                                                          <  SRR4302540.9542545/74‑1 (MQ=60)

   gtttgaaaaaaaaacccgttttagggcataAATGCTTACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCA                                                                                          <  SRR4302540.3517694/64‑1 (MQ=60)

   gtttaaaaaaaaaaccctgtttatagaatgattccttacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCA                                                                                          <  SRR4302540.7823978/55‑1 (MQ=60)

    CTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAA                                                                                         >  SRR4302540.9416321/1‑94 (MQ=60)

     ttaaaaaaaaaaccttgtttatagaagaAATGCTTACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAAC                                                                                        <  SRR4302540.2170489/66‑1 (MQ=60)

     tagaaaaaaaaaacgtgtttaaagcaagattcctgccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAAC                                                                                        <  SRR4302540.4336139/57‑1 (MQ=60)

     TTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAAC                                                                                        >  SRR4302540.7456531/1‑94 (MQ=60)

      ttaataaaagaccttggtttgaagtgtaatcttaccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACG                                                                                       <  SRR4302540.492617/58‑1 (MQ=60)

      ttaaaaaagactCCTGGTTATAGCAAGAATGCATCCCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACG                                                                                       <  SRR4302540.492616/82‑1 (MQ=60)

      tgaaaaaagacctggggtcagagcaggatcggtgacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACG                                                                                       <  SRR4302540.1280776/58‑1 (MQ=60)

      tagaaaaagaacctTGGTTATAGCATGAATGCTTCCCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACG                                                                                       <  SRR4302540.1280777/80‑1 (MQ=60)

      tagaaaaaaaagcgtgtttaaagaatgatgccttacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACG                                                                                       <  SRR4302540.338976/58‑1 (MQ=60)

      taaaaaaagaaccctgttaagagcatgaatgctaccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACG                                                                                       <  SRR4302540.938527/58‑1 (MQ=60)

      taaaaaaaaaaacttggttaaagcataattgctcAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACG                                                                                       <  SRR4302540.3071885/60‑1 (MQ=60)

      TTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACG                                                                                       >  SRR4302540.5818290/1‑94 (MQ=60)

       taaaaaaaaaccttgtttaaagcataaatgctaccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGT                                                                                      <  SRR4302540.4526/59‑1 (MQ=60)

       TGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGT                                                                                      >  SRR4302540.4799143/1‑94 (MQ=60)

         aaaaagaacctggtTTATAGCGTGAATGCTCACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTAT                                                                                    <  SRR4302540.1167205/80‑1 (MQ=60)

         aaaaaaaaccttggttatagaagaattcctcacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTAT                                                                                    <  SRR4302540.3941285/61‑1 (MQ=60)

         aaaaaaaaccctttttataacataaattactccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTAT                                                                                    <  SRR4302540.1519373/61‑1 (MQ=60)

         aaaaaaaaagactgttatacgaaaaatcattagCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTAT                                                                                    <  SRR4302540.8862883/61‑1 (MQ=60)

         ATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTAT                                                                                    >  SRR4302540.6067571/1‑94 (MQ=60)

         ATAAACAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTAT                                                                                    >  SRR4302540.6067570/1‑94 (MQ=60)

          aaaaaaaccctgtttaaagcaggactcctcccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATT                                                                                   <  SRR4302540.5534944/62‑1 (MQ=60)

          aaaaaAAACCTGGTTATAGAATAAATGCTCACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATT                                                                                   <  SRR4302540.9745978/89‑1 (MQ=60)

          TAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATT                                                                                   >  SRR4302540.8670990/1‑94 (MQ=60)

           AAAGAACCGTGGTTATAGAATGAATCCTAACCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTG                                                                                  <  SRR4302540.1130485/94‑1 (MQ=60)

            AAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGA                                                                                 >  SRR4302540.5206630/1‑94 (MQ=60)

            AAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGA                                                                                 >  SRR4302540.7477007/1‑94 (MQ=60)

             AGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAG                                                                                >  SRR4302540.9273877/1‑94 (MQ=60)

               acccttgttaaaagaatatctcctcccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCT                                                                              <  SRR4302540.2341935/67‑1 (MQ=60)

               aacccttgataaagtagcattgtttccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCT                                                                              <  SRR4302540.9990822/67‑1 (MQ=60)

                AACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTT                                                                             >  SRR4302540.5954939/1‑94 (MQ=60)

                AACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTT                                                                             >  SRR4302540.7276489/1‑94 (MQ=60)

                AACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTT                                                                             >  SRR4302540.7276490/1‑94 (MQ=60)

                   ttgtttatagaaggaatgctcacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATC                                                                          <  SRR4302540.3885698/71‑1 (MQ=60)

                   CTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATC                                                                          >  SRR4302540.7221553/1‑94 (MQ=60)

                    ttcttatagtataagtgcatacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCA                                                                         <  SRR4302540.4697843/72‑1 (MQ=60)

                    tgtttatagcagaattcctcccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCA                                                                         <  SRR4302540.505685/72‑1 (MQ=60)

                    tgttaatacaataattcttaacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCA                                                                         <  SRR4302540.1640130/72‑1 (MQ=60)

                    TGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCA                                                                         >  SRR4302540.5211324/1‑94 (MQ=60)

                     GGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCAT                                                                        >  SRR4302540.341469/1‑94 (MQ=60)

                     GGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCAT                                                                        >  SRR4302540.2512525/1‑94 (MQ=60)

                     GGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCAT                                                                        >  SRR4302540.8029299/1‑94 (MQ=60)

                      GTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATC                                                                       >  SRR4302540.3482281/1‑94 (MQ=60)

                      GTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATC                                                                       >  SRR4302540.10089992/1‑94 (MQ=60)

                        ttgataattattgcacacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCAT                                                                     <  SRR4302540.3261198/76‑1 (MQ=60)

                        tctgaaatgattgctaacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCAT                                                                     <  SRR4302540.581655/76‑1 (MQ=60)

                        aaaacatcaatggcatccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCAT                                                                     <  SRR4302540.3261199/76‑1 (MQ=60)

                         atgtaaagaatcctaccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATC                                                                    <  SRR4302540.9077496/77‑1 (MQ=60)

                         agagcataaatgctaccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATC                                                                    <  SRR4302540.8085574/77‑1 (MQ=60)

                         acacgataaatcctcacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATC                                                                    <  SRR4302540.8837961/77‑1 (MQ=60)

                         ATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATC                                                                    >  SRR4302540.2416892/1‑94 (MQ=60)

                         ATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATC                                                                    >  SRR4302540.3895175/1‑94 (MQ=60)

                          aagtagaaatgtccacCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCA                                                                   <  SRR4302540.7989007/78‑1 (MQ=60)

                          TAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCA                                                                   >  SRR4302540.5043551/1‑94 (MQ=60)

                          TAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCA                                                                   >  SRR4302540.5043552/1‑94 (MQ=60)

                             aatgaattctcccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAAC                                                                <  SRR4302540.301859/81‑1 (MQ=60)

                             aagaactccttccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAAC                                                                <  SRR4302540.9942987/81‑1 (MQ=60)

                             aagaaatcctcccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAAC                                                                <  SRR4302540.398940/81‑1 (MQ=60)

                             CATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAAC                                                                >  SRR4302540.5419856/1‑94 (MQ=60)

                             CATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAAC                                                                >  SRR4302540.3635644/1‑94 (MQ=60)

                              atgactgctaccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACT                                                               <  SRR4302540.9228265/82‑1 (MQ=60)

                              ATGAATGCTTCCCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACT                                                               <  SRR4302540.6969315/94‑1 (MQ=60)

                               TGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTG                                                              >  SRR4302540.7408582/1‑94 (MQ=60)

                               TGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTG                                                              >  SRR4302540.148385/1‑94 (MQ=60)

                                GAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGGAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGC                                                             >  SRR4302540.10009331/1‑94 (MQ=60)

                                 aatcctcccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCC                                                            <  SRR4302540.7733815/85‑1 (MQ=60)

                                  atgttcccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCA                                                           <  SRR4302540.7279135/86‑1 (MQ=60)

                                   tccagcgccctCCGTAGCACGCATGCCGCTGGTAACAATCGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAG                                                          <  SRR4302540.5163256/83‑1 (MQ=60)

                                   TGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAG                                                          >  SRR4302540.9120040/1‑94 (MQ=60)

                                    GCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGC                                                         >  SRR4302540.5847303/1‑94 (MQ=60)

                                     ctcccCCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCG                                                        <  SRR4302540.7249527/89‑1 (MQ=60)

                                       TAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCACACTGCCAGCGGC                                                      >  SRR4302540.6118104/1‑94 (MQ=60)

                                       TAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGC                                                      >  SRR4302540.6118105/1‑94 (MQ=60)

                                         ACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCT                                                    >  SRR4302540.3858678/1‑94 (MQ=60)

                                          CCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTT                                                   <  SRR4302540.9485547/94‑1 (MQ=60)

                                           CCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTC                                                  >  SRR4302540.8022956/1‑94 (MQ=60)

                                           CCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTC                                                  >  SRR4302540.3549408/1‑94 (MQ=60)

                                             GCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACGTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGA                                                >  SRR4302540.865963/1‑94 (MQ=60)

                                              CCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCATCGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGAC                                               <  SRR4302540.431836/94‑1 (MQ=60)

                                              CCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGAC                                               >  SRR4302540.7061644/1‑94 (MQ=60)

                                              CCGTAACACGCGTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGAC                                               <  SRR4302540.8986666/94‑1 (MQ=60)

                                               CGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACA                                              >  SRR4302540.3253962/1‑94 (MQ=60)

                                               CGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACA                                              <  SRR4302540.817627/94‑1 (MQ=60)

                                                GTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAA                                             >  SRR4302540.8233413/1‑94 (MQ=60)

                                                GTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAA                                             <  SRR4302540.9019651/94‑1 (MQ=60)

                                                GTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAA                                             >  SRR4302540.4507906/1‑94 (MQ=60)

                                                   ACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATA                                          >  SRR4302540.9410683/1‑94 (MQ=60)

                                                    CACTCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAC                                         <  SRR4302540.9694783/94‑1 (MQ=60)

                                                    CACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAC                                         <  SRR4302540.7027116/94‑1 (MQ=60)

                                                    CACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAC                                         >  SRR4302540.8848075/1‑94 (MQ=60)

                                                    CACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAC                                         <  SRR4302540.8490350/94‑1 (MQ=60)

                                                    CACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAC                                         <  SRR4302540.2801382/94‑1 (MQ=60)

                                                    CACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATAC                                         <  SRR4302540.4454615/94‑1 (MQ=60)

                                                      CGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCC                                       >  SRR4302540.1298705/1‑94 (MQ=60)

                                                      CGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCC                                       >  SRR4302540.1298706/1‑94 (MQ=60)

                                                      CGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCC                                       >  SRR4302540.4446908/1‑94 (MQ=60)

                                                      CGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCC                                       <  SRR4302540.6264962/94‑1 (MQ=60)

                                                         TTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTG                                    <  SRR4302540.9689912/94‑1 (MQ=60)

                                                          TGCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGC                                   <  SRR4302540.7756515/94‑1 (MQ=60)

                                                           GCCGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCG                                  <  SRR4302540.3500888/94‑1 (MQ=60)

                                                             CGCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTC                                >  SRR4302540.1914268/1‑94 (MQ=60)

                                                              GCTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCT                               <  SRR4302540.4577919/94‑1 (MQ=60)

                                                               CTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACACATACCCGTGCGTCTG                              >  SRR4302540.7471746/1‑94 (MQ=60)

                                                               CTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTG                              <  SRR4302540.5213653/94‑1 (MQ=60)

                                                               CTGGTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTG                              <  SRR4302540.1739375/94‑1 (MQ=60)

                                                                  GTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCG                           >  SRR4302540.8602480/1‑94 (MQ=60)

                                                                  GTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCG                           >  SRR4302540.4094373/1‑94 (MQ=60)

                                                                  GTAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCG                           >  SRR4302540.3011113/1‑94 (MQ=60)

                                                                   TAACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGC                          >  SRR4302540.4883983/1‑94 (MQ=60)

                                                                     ACAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTG                        <  SRR4302540.9409294/94‑1 (MQ=60)

                                                                      CAAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGC                       <  SRR4302540.171335/94‑1 (MQ=60)

                                                                       AAACGTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCT                      <  SRR4302540.2949948/94‑1 (MQ=60)

                                                                           GTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATA                  >  SRR4302540.1138622/1‑94 (MQ=60)

                                                                           GTCCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGATGATA                  >  SRR4302540.1138623/1‑94 (MQ=60)

                                                                             CCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACT                <  SRR4302540.9337082/94‑1 (MQ=60)

                                                                             CCCAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTG‑CAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTA               >  SRR4302540.7615340/1‑94 (MQ=60)

                                                                               CAGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTAC              >  SRR4302540.2069205/1‑94 (MQ=60)

                                                                                AGCACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACT             <  SRR4302540.5792231/94‑1 (MQ=60)

                                                                                  CACCTAAATGACGCAACGTATTGAGCTTATCATCCTCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTC           <  SRR4302540.6281224/94‑1 (MQ=60)

                                                                                  CACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTC           <  SRR4302540.6183577/94‑1 (MQ=60)

                                                                                   ACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCG          >  SRR4302540.127886/1‑94 (MQ=60)

                                                                                   ACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCG          >  SRR4302540.8224451/1‑94 (MQ=60)

                                                                                   ACCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCG          >  SRR4302540.2642843/1‑94 (MQ=60)

                                                                                    CCTAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGC         >  SRR4302540.9601633/1‑94 (MQ=60)

                                                                                      TAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGTCGCTGCTGATACTACTTCGCCA       >  SRR4302540.9092618/1‑94 (MQ=60)

                                                                                      TAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCA       <  SRR4302540.2748338/94‑1 (MQ=60)

                                                                                      TAAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGACACTACTTCGCCA       >  SRR4302540.9225554/1‑94 (MQ=60)

                                                                                       AAATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAA      <  SRR4302540.8618633/94‑1 (MQ=60)

                                                                                        AATGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAA     <  SRR4302540.2174776/94‑1 (MQ=60)

                                                                                          TGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACA   <  SRR4302540.1179294/94‑1 (MQ=60)

                                                                                          TGACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACA   <  SRR4302540.9230878/94‑1 (MQ=60)

                                                                                           GACGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAG  <  SRR4302540.6069777/94‑1 (MQ=60)

GAGGCTTTGATAAAGAAACCTGGTTATAGCATGAATGCTTAACCCGCCGTAACACGCTTGCCGCTGGTAACAAACGTCCCAGCACCTAAATGATGCAACGTATTGAGCTTATCATCATCAAACTGCCAGCGGCCTTCGACAAATACCCGTGCGTCTGCCGCTGCTGATACTACTTCGCCAAACAG  >  NC_000913/1531268‑1531452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`