Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,549,144 T→C G220G (GGT→GGC)  fdnH → formate dehydrogenase‑N, nitrate‑inducible, iron‑sulfur beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,549,1440TC100.0% 598.1 / NA 188G220G (GGT→GGC) fdnHformate dehydrogenase‑N, nitrate‑inducible, iron‑sulfur beta subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (86/102);  total (86/102)

Only 100 of 199 total aligned reads displayed.

GGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGT  >  NC_000913/1549054‑1549233
                                                                                          |                                                                                         
ggAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTcggcgg                                                                                         >  2:235684/1‑93 (MQ=255)
 gagaTGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTcggcgga                                                                                        >  2:5189433/1‑92 (MQ=255)
     tGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGc                                                                                    <  1:1191809/93‑1 (MQ=255)
     tGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGc                                                                                    >  1:1279361/1‑93 (MQ=255)
      gCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCa                                                                                   <  2:7616348/93‑1 (MQ=255)
      gCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCa                                                                                   <  1:2821688/93‑1 (MQ=255)
       cTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCAc                                                                                  <  1:7333071/93‑1 (MQ=255)
         ggAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGt                                                                                <  1:6980370/93‑1 (MQ=255)
         ggAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGt                                                                                >  1:5284720/1‑93 (MQ=255)
         ggAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGt                                                                                >  2:9027557/1‑93 (MQ=255)
          gAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGAACGtt                                                                               >  2:5118836/1‑93 (MQ=255)
           aGCTGGCGGAACAGCGCGTGGCGAAACTGGAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTa                                                                              <  2:455758/93‑1 (MQ=255)
               ggCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTa                                                                          <  1:589958/93‑1 (MQ=255)
                gCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTAc                                                                         <  2:8515516/93‑1 (MQ=255)
                gCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGGACGCACGTTATGTAc                                                                         >  2:3876555/1‑93 (MQ=255)
                  ggAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGt                                                                       <  2:1815701/93‑1 (MQ=255)
                  ggAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGt                                                                       >  2:9391995/1‑93 (MQ=255)
                  ggAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGt                                                                       >  1:8641599/1‑93 (MQ=255)
                   gAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGtg                                                                      <  2:9752482/93‑1 (MQ=255)
                   gAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGtg                                                                      >  1:9613485/1‑93 (MQ=255)
                   gAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGtg                                                                      >  2:6596820/1‑93 (MQ=255)
                       aGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGtgctgc                                                                  >  2:9505780/1‑93 (MQ=255)
                         cgcgTGGCGAAACTGAAAGAGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCAt                                                                >  1:9743660/1‑93 (MQ=255)
                            gTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCAc                                                             <  1:5469814/93‑1 (MQ=255)
                            gTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCAc                                                             >  2:2073426/1‑93 (MQ=255)
                             tGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACg                                                            >  1:1883570/1‑93 (MQ=255)
                              ggCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGc                                                           >  2:3703185/1‑93 (MQ=255)
                              ggCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGc                                                           <  1:3486836/93‑1 (MQ=255)
                               gCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGcc                                                          <  1:2124543/93‑1 (MQ=255)
                               gCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGcc                                                          >  2:2272488/1‑93 (MQ=255)
                               gCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGcc                                                          >  2:3006243/1‑93 (MQ=255)
                                    aCTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGCTCa                                                     >  2:7676576/1‑93 (MQ=255)
                                    aCTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCa                                                     >  2:2749291/1‑93 (MQ=255)
                                     cTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAg                                                    <  2:1370622/93‑1 (MQ=255)
                                      tGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGc                                                   <  1:3163462/93‑1 (MQ=255)
                                      tGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGc                                                   <  2:6082422/93‑1 (MQ=255)
                                      tGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGc                                                   >  1:1802953/1‑93 (MQ=255)
                                       gAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGcc                                                  >  1:8116868/1‑93 (MQ=255)
                                       gAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGcc                                                  >  1:10007363/1‑93 (MQ=255)
                                        aaaGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCg                                                 <  1:63970/93‑1 (MQ=255)
                                        aaaGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCg                                                 <  1:3595996/93‑1 (MQ=255)
                                        aaaGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCg                                                 <  2:5939909/93‑1 (MQ=255)
                                         aaGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCgg                                                >  2:5008570/1‑93 (MQ=255)
                                          aGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGa                                               >  1:835117/1‑93 (MQ=255)
                                          aGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGa                                               >  1:1423015/1‑93 (MQ=255)
                                           gcgcGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAg                                              <  2:4516694/93‑1 (MQ=255)
                                            cgcgTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGc                                             <  1:6302920/93‑1 (MQ=255)
                                            cgcgTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGc                                             <  2:8670482/93‑1 (MQ=255)
                                              cgTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTg                                           <  2:911209/93‑1 (MQ=255)
                                              cgTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTg                                           >  2:8181304/1‑93 (MQ=255)
                                              cgTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGCAGCTg                                           >  2:9595694/1‑93 (MQ=255)
                                               gTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGt                                          >  2:1240017/1‑93 (MQ=255)
                                               gTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGt                                          <  2:7393295/93‑1 (MQ=255)
                                               gTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGt                                          >  1:3063093/1‑93 (MQ=255)
                                                tGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTa                                         <  1:4627918/93‑1 (MQ=255)
                                                 ggTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTAt                                        <  1:2700825/93‑1 (MQ=255)
                                                  gTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATc                                       <  1:9376150/93‑1 (MQ=255)
                                                    tACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCAc                                     <  1:9852870/93‑1 (MQ=255)
                                                     aCGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACg                                    <  1:7571954/93‑1 (MQ=255)
                                                       gAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGt                                  >  2:2233729/1‑93 (MQ=255)
                                                        ctcgtGCTGGCGTCTACCCCCCGGAAGGGGTCGGCGGTCCGCCCGTTGTGTCCGTGCTGCATCACGCCGCTCCGCCGGCGCTGTGTCACGGTc                                 <  1:8946530/89‑1 (MQ=255)
                                                        aaCATCCTGTCGTTGCCACCCCGGAAGGGCTCGG‑CGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCt                                <  2:4053399/93‑1 (MQ=255)
                                                         aCATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCt                                <  1:3576621/93‑1 (MQ=255)
                                                         aCATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCt                                <  1:9941901/93‑1 (MQ=255)
                                                         aCATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCt                                <  2:5636476/93‑1 (MQ=255)
                                                           aTGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGc                              <  2:702013/93‑1 (MQ=255)
                                                            tGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGcc                             >  2:1599542/1‑93 (MQ=255)
                                                            tGCGGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGcc                             <  2:685064/93‑1 (MQ=255)
                                                             tcTGGCGTCTACAACCCGGATGGGGGCGGCCGTACGCACGTTATGGACGTGCTGCATCACGGCGATCAGCCCTAGCTGCATCACGGACGGCCg                            >  2:4854633/2‑93 (MQ=255)
                                                             gCTGGCGTCTACAGCCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCg                            <  1:6955100/93‑1 (MQ=255)
                                                             gCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCg                            <  1:4221326/93‑1 (MQ=255)
                                                                ggCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGaaa                         >  2:425405/1‑93 (MQ=255)
                                                                ggCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGaaa                         <  2:2575374/93‑1 (MQ=255)
                                                                 gCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAg                        <  2:3409726/93‑1 (MQ=255)
                                                                  cGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGa                       <  1:6653847/93‑1 (MQ=255)
                                                                   gTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGAt                      <  1:4031266/93‑1 (MQ=255)
                                                                   gTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGAt                      <  1:632960/93‑1 (MQ=255)
                                                                   gTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGAt                      >  1:57346/1‑93 (MQ=255)
                                                                    tCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGAtg                     <  2:7504141/93‑2 (MQ=255)
                                                                    tCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATc                     >  1:3819435/1‑93 (MQ=255)
                                                                      tACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCg                   <  2:3893401/93‑1 (MQ=255)
                                                                      tACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCg                   >  2:9869338/1‑93 (MQ=255)
                                                                      tACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCg                   <  2:9572399/93‑1 (MQ=255)
                                                                         aaCCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCTTCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAg                <  2:8973530/93‑1 (MQ=255)
                                                                         aaCCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAg                <  2:6959476/93‑1 (MQ=255)
                                                                         aaCCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAg                >  1:6502950/1‑93 (MQ=255)
                                                                          aCCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGa               <  1:5314312/93‑1 (MQ=255)
                                                                            ccGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATc             <  1:503410/93‑1 (MQ=255)
                                                                            ccGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATc             <  1:1368619/93‑1 (MQ=255)
                                                                            ccGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATc             <  1:3117448/93‑1 (MQ=255)
                                                                            ccGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATc             <  2:1614366/93‑1 (MQ=255)
                                                                             cGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCg            <  1:1615074/93‑1 (MQ=255)
                                                                             cGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCg            <  2:5549653/93‑1 (MQ=255)
                                                                                aaGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGaca         >  2:1551159/1‑93 (MQ=255)
                                                                                aaGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGaca         >  2:3679737/1‑93 (MQ=255)
                                                                                aaGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGaca         >  2:773287/1‑93 (MQ=255)
                                                                                aaGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGaca         <  1:6163127/93‑1 (MQ=255)
                                                                                  ggggTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACAcc       >  1:1833324/1‑93 (MQ=255)
                                                                                  ggggTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACAcc       <  1:849951/93‑1 (MQ=255)
                                                                                       cggcggTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGt  >  1:743239/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGT  >  NC_000913/1549054‑1549233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGC  >  NC_000913/1549051‑1549237
                                                                                             |                                                                                             
GAAGGAGATGCGGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGC                                                                                               <  SRR4302540.4014327/94‑1 (MQ=60)
       ATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTGGGCGGTAAGC                                                                                        >  SRR4302540.861739/1‑94 (MQ=60)
       ATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGC                                                                                        >  SRR4302540.1292547/1‑94 (MQ=60)
        TGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCA                                                                                       <  SRR4302540.1204094/94‑1 (MQ=60)
         GCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCAC                                                                                      >  SRR4302540.843200/1‑94 (MQ=60)
         GCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCAC                                                                                      <  SRR4302540.2852168/94‑1 (MQ=60)
          CTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACG                                                                                     <  SRR4302540.7414926/94‑1 (MQ=60)
          CTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACG                                                                                     <  SRR4302540.414755/94‑1 (MQ=60)
          CTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACG                                                                                     >  SRR4302540.529856/1‑94 (MQ=60)
           TGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGT                                                                                    >  SRR4302540.6415319/1‑94 (MQ=60)
           TGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGT                                                                                    >  SRR4302540.5342468/1‑94 (MQ=60)
            GGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTT                                                                                   <  SRR4302540.7057911/94‑1 (MQ=60)
               GCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATG                                                                                <  SRR4302540.7517478/94‑1 (MQ=60)
                 TGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTA                                                                              >  SRR4302540.2907204/1‑94 (MQ=60)
                  GGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTAC                                                                             <  SRR4302540.596316/94‑1 (MQ=60)
                    CGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGT                                                                           >  SRR4302540.8741981/1‑94 (MQ=60)
                    CGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGT                                                                           >  SRR4302540.8742243/1‑94 (MQ=60)
                     GGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTG                                                                          >  SRR4302540.9726831/1‑94 (MQ=60)
                          AGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCA                                                                     >  SRR4302540.4419441/1‑94 (MQ=60)
                           GCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCAT                                                                    <  SRR4302540.5439116/94‑1 (MQ=60)
                           GCGCGTGGCGAAACTGAAAGAGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCAT                                                                    >  SRR4302540.9858019/1‑94 (MQ=60)
                             GCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCA                                                                  >  SRR4302540.1610796/1‑94 (MQ=60)
                               GTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACG                                                                >  SRR4302540.1904207/1‑94 (MQ=60)
                               GTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACG                                                                <  SRR4302540.5530025/94‑1 (MQ=60)
                               GTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACG                                                                <  SRR4302540.1356851/94‑1 (MQ=60)
                               GTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACG                                                                >  SRR4302540.9791796/1‑94 (MQ=60)
                                TGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGC                                                               >  SRR4302540.3618926/1‑94 (MQ=60)
                                 GGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCC                                                              <  SRR4302540.3524806/94‑1 (MQ=60)
                                  GCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCG                                                             <  SRR4302540.2147339/94‑1 (MQ=60)
                                     AAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATC                                                          <  SRR4302540.125776/94‑1 (MQ=60)
                                      AACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCA                                                         >  SRR4302540.3336748/1‑94 (MQ=60)
                                      AACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCA                                                         <  SRR4302540.168221/94‑1 (MQ=60)
                                      AACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCA                                                         <  SRR4302540.6504505/94‑1 (MQ=60)
                                       ACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTCCAACCCGAAAGGGGACGGCGGTACGCACGTTAGGTCCGTGCTGCATCACGCCGATCAG                                                        >  SRR4302540.4921144/1‑94 (MQ=60)
                                       ACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAG                                                        >  SRR4302540.4921143/1‑94 (MQ=60)
                                       ACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAG                                                        <  SRR4302540.1857601/94‑1 (MQ=60)
                                        CTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGC                                                       >  SRR4302540.1395890/1‑94 (MQ=60)
                                        CTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGC                                                       >  SRR4302540.1822590/1‑94 (MQ=60)
                                        CTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGC                                                       >  SRR4302540.7055815/1‑94 (MQ=60)
                                         TGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCC                                                      <  SRR4302540.3197871/94‑1 (MQ=60)
                                         TGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCC                                                      >  SRR4302540.8211064/1‑94 (MQ=60)
                                         TGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCC                                                      >  SRR4302540.10123913/1‑94 (MQ=60)
                                           AAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGG                                                    <  SRR4302540.3635290/94‑1 (MQ=60)
                                           AAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGG                                                    <  SRR4302540.64613/94‑1 (MQ=60)
                                            AAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGA                                                   >  SRR4302540.843987/1‑94 (MQ=60)
                                            AAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGA                                                   >  SRR4302540.1438419/1‑94 (MQ=60)
                                              GCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGC                                                 >  SRR4302540.5945217/1‑94 (MQ=60)
                                               CGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCT                                                <  SRR4302540.6373108/94‑1 (MQ=60)
                                                 CGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGT                                              <  SRR4302540.7495054/94‑1 (MQ=60)
                                                 CGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGT                                              >  SRR4302540.3096696/1‑94 (MQ=60)
                                                   TGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTAT                                            <  SRR4302540.4677496/94‑1 (MQ=60)
                                                    GGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATC                                           <  SRR4302540.2729970/94‑1 (MQ=60)
                                                    GGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATC                                           <  SRR4302540.5349526/94‑1 (MQ=60)
                                                     GTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCA                                          <  SRR4302540.9487764/94‑1 (MQ=60)
                                                      TTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCAC                                         <  SRR4302540.1955020/94‑1 (MQ=60)
                                                       TACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACG                                        <  SRR4302540.9968021/94‑1 (MQ=60)
                                                       TACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACG                                        <  SRR4302540.2837576/94‑1 (MQ=60)
                                                        ACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGG                                       <  SRR4302540.7656897/94‑1 (MQ=60)
                                                        ACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGG                                       <  SRR4302540.2679934/94‑1 (MQ=60)
                                                            ACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTG                                   <  SRR4302540.3615686/94‑1 (MQ=60)
                                                            ACATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTG                                   <  SRR4302540.10058012/94‑1 (MQ=60)
                                                             CATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGC                                  >  SRR4302540.3865535/1‑94 (MQ=60)
                                                              ATGCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCC                                 >  SRR4302540.7230502/1‑94 (MQ=60)
                                                                GCTGGCGTCTACAGCCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGA                               <  SRR4302540.7032483/94‑1 (MQ=60)
                                                                GCTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGA                               <  SRR4302540.4267283/94‑1 (MQ=60)
                                                                 CTGGCGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAA                              >  SRR4302540.9615640/1‑94 (MQ=60)
                                                                     CGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGAT                          <  SRR4302540.6727441/94‑1 (MQ=60)
                                                                     CGTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGAT                          >  SRR4302540.57921/1‑94 (MQ=60)
                                                                      GTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATC                         <  SRR4302540.4074739/94‑1 (MQ=60)
                                                                      GTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATC                         <  SRR4302540.639689/94‑1 (MQ=60)
                                                                      GTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATC                         <  SRR4302540.639688/94‑1 (MQ=60)
                                                                      GTCTACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATC                         >  SRR4302540.3861013/1‑94 (MQ=60)
                                                                          ACAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAA                     >  SRR4302540.9257191/1‑94 (MQ=60)
                                                                           CAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAG                    >  SRR4302540.6575237/1‑94 (MQ=60)
                                                                           CAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAG                    <  SRR4302540.8125696/94‑1 (MQ=60)
                                                                           CAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAG                    >  SRR4302540.556705/1‑94 (MQ=60)
                                                                           CAACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAG                    >  SRR4302540.6575238/1‑94 (MQ=60)
                                                                             ACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGAT                  <  SRR4302540.9900159/94‑1 (MQ=60)
                                                                             ACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGAT                  <  SRR4302540.5983676/94‑1 (MQ=60)
                                                                             ACCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGAT                  <  SRR4302540.5372506/94‑1 (MQ=60)
                                                                             ACCCAAAAGGGATCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATAAGCCGGAGCTGTATCACGGTCTGCAGAAAGATCCGAAGAT                  <  SRR4302540.9064826/94‑1 (MQ=60)
                                                                              CCCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATC                 <  SRR4302540.3611619/94‑1 (MQ=60)
                                                                               CCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCG                <  SRR4302540.508803/94‑1 (MQ=60)
                                                                               CCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCG                <  SRR4302540.1383115/94‑1 (MQ=60)
                                                                               CCGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCG                <  SRR4302540.3151530/94‑1 (MQ=60)
                                                                                CGGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGA               <  SRR4302540.1632793/94‑1 (MQ=60)
                                                                                 GGAAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGAC              >  SRR4302540.8533022/1‑94 (MQ=60)
                                                                                   AAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACAC            <  SRR4302540.6231804/94‑1 (MQ=60)
                                                                                   AAGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACAC            <  SRR4302540.6231803/94‑1 (MQ=60)
                                                                                    AGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGCAAGATCCGAAGATCGACCCC           >  SRR4302540.1853386/1‑94 (MQ=60)
                                                                                    AGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACC           >  SRR4302540.1853387/1‑94 (MQ=60)
                                                                                    AGGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAAATCGACACC           <  SRR4302540.6893875/94‑1 (MQ=60)
                                                                                     GGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCT          <  SRR4302540.858971/94‑1 (MQ=60)
                                                                                     GGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCT          >  SRR4302540.3780926/1‑94 (MQ=60)
                                                                                     GGGGTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCT          <  SRR4302540.10045148/94‑1 (MQ=60)
                                                                                        GTCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGG       <  SRR4302540.3116550/94‑1 (MQ=60)
                                                                                         TCGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGT      >  SRR4302540.751163/1‑94 (MQ=60)
                                                                                          CGGCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTA     >  SRR4302540.3960712/1‑94 (MQ=60)
                                                                                            GCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAG   <  SRR4302540.8020726/94‑1 (MQ=60)
                                                                                            GCGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAG   >  SRR4302540.9858930/1‑94 (MQ=60)
                                                                                             CGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGC  >  SRR4302540.5269051/1‑94 (MQ=60)
                                                                                             CGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGC  >  SRR4302540.5903185/1‑94 (MQ=60)
                                                                                             |                                                                                             
GAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTGGCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGTGGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTGCCGAAAGATCCGAAGATCGACACCTCGGTAAGC  >  NC_000913/1549051‑1549237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑