Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,549,298 T→C L272L (TTG→CTG)  fdnH → formate dehydrogenase‑N, nitrate‑inducible, iron‑sulfur beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,549,2980TC99.6% 725.1 / ‑5.4 225L272L (TTG→CTG) fdnHformate dehydrogenase‑N, nitrate‑inducible, iron‑sulfur beta subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (110/114);  total (111/114)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 231 total aligned reads displayed.

GAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCG  >  NC_000913/1549207‑1549387
                                                                                           |                                                                                         
   aGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGAt                                                                                       >  1:1166079/1‑93 (MQ=255)
   aGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGAt                                                                                       >  2:8529646/1‑93 (MQ=255)
   aGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGAt                                                                                       >  2:7056885/1‑93 (MQ=255)
    gATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGAtt                                                                                      >  2:4617724/1‑93 (MQ=255)
     aTCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGAttt                                                                                     <  2:7092071/93‑1 (MQ=255)
      tCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGAtttt                                                                                    <  1:9869339/93‑1 (MQ=255)
       ccGAAGATCGCCACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTc                                                                                   >  2:1815436/1‑93 (MQ=255)
       ccGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCGCTTTTGCCGGGCTGATTTTc                                                                                   <  2:1279360/93‑1 (MQ=255)
        cGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTcc                                                                                  <  1:3957126/93‑1 (MQ=255)
        cGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTcc                                                                                  <  2:1450825/93‑1 (MQ=255)
         gAAGATCGACCCCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCa                                                                                 <  1:2233729/93‑1 (MQ=255)
         gAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCa                                                                                 >  1:2990047/1‑93 (MQ=255)
         gAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCa                                                                                 >  2:3883625/1‑93 (MQ=255)
          aaGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCAc                                                                                >  1:6854634/1‑93 (MQ=255)
           aGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACt                                                                               >  2:5201000/1‑93 (MQ=255)
              tCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACa                                                                            >  1:4712490/1‑93 (MQ=255)
              tCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACa                                                                            <  1:6116384/93‑1 (MQ=255)
              tCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACa                                                                            <  1:3401758/93‑1 (MQ=255)
                 acacCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCg                                                                         >  1:1423446/1‑93 (MQ=255)
                  cacCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCgg                                                                        <  1:2131352/93‑1 (MQ=255)
                   acCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGt                                                                       >  1:3718109/1‑93 (MQ=255)
                   acCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGt                                                                       >  2:1945272/1‑93 (MQ=255)
                     cTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTAt                                                                     <  2:4536972/93‑1 (MQ=255)
                     cTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTAt                                                                     <  2:10007362/93‑1 (MQ=255)
                      tCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTAtt                                                                    >  2:590719/1‑93 (MQ=255)
                      tCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTAtt                                                                    >  2:7986700/1‑93 (MQ=255)
                         gTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGc                                                                 >  2:5600805/1‑93 (MQ=255)
                           aaGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGccc                                                               >  1:5241135/1‑93 (MQ=255)
                            aGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCg                                                              <  2:3111585/93‑1 (MQ=255)
                            aGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCg                                                              <  1:3248988/93‑1 (MQ=255)
                              ccTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGaa                                                            <  1:1506328/93‑1 (MQ=255)
                               ctttgGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAt                                                           <  1:2637823/90‑1 (MQ=255)
                               cTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGaaa                                                           >  1:8031438/1‑92 (MQ=255)
                               cTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAt                                                           <  1:2076015/93‑1 (MQ=255)
                                tgtgGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATa                                                          >  1:5757348/1‑93 (MQ=255)
                                 gtgGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTACACTACATCGGTATTGGCCCGAATaa                                                         >  1:8247694/1‑93 (MQ=255)
                                  tgGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGCTTTTCAACTACATCGGTATTGGCCCGAATAAg                                                        >  1:5818926/1‑93 (MQ=255)
                                   ggAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAgg                                                       <  2:1172328/93‑1 (MQ=255)
                                      aaGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAg                                                    <  2:9147330/93‑1 (MQ=255)
                                           gcgTTGAAACCTCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGAc                                               <  2:2047589/93‑1 (MQ=255)
                                           gcgTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGAc                                               <  2:3040870/93‑1 (MQ=255)
                                            cgTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACg                                              >  1:8375272/1‑93 (MQ=255)
                                              tggAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGTAAGTGGACGAt                                            <  2:5298297/91‑1 (MQ=255)
                                               tGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATg                                           >  2:1590168/1‑93 (MQ=255)
                                               tGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATg                                           <  2:9744564/93‑1 (MQ=255)
                                               tGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATg                                           <  1:370147/93‑1 (MQ=255)
                                                gAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGa                                          <  1:9133341/93‑1 (MQ=255)
                                                gAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGa                                          <  1:425405/93‑1 (MQ=255)
                                                gAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGa                                          <  2:728816/93‑1 (MQ=255)
                                                gAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGa                                          <  2:7446447/93‑1 (MQ=255)
                                                 aaaCCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGAc                                         >  1:9503215/1‑93 (MQ=255)
                                                    ccGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACgag                                      >  1:4008389/1‑93 (MQ=255)
                                                    ccGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACgag                                      <  2:3300618/93‑1 (MQ=255)
                                                     cGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACgagg                                     >  1:9808936/1‑93 (MQ=255)
                                                      gCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACgagga                                    <  1:5189434/93‑1 (MQ=255)
                                                       cTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACgaggag                                   >  2:2705885/1‑93 (MQ=255)
                                                        tGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGAGGACGATGACGaggagg                                  >  2:8977718/1‑93 (MQ=255)
                                                         ggCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAggagga                                 <  1:3926467/93‑1 (MQ=255)
                                                         ggCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAggagga                                 <  2:448029/93‑1 (MQ=255)
                                                           cAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGatc                               >  1:1775046/1‑93 (MQ=255)
                                                            aGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGatca                              >  2:6940070/1‑93 (MQ=255)
                                                             gCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGatcat                             <  1:9299654/93‑1 (MQ=255)
                                                             gCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGatcat                             >  2:1307904/1‑93 (MQ=255)
                                                             gCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAATGGACGATGACGAGGAGGctcat                             >  1:6596129/1‑93 (MQ=255)
                                                               ggctggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGAtcatca                           >  2:6400334/1‑93 (MQ=255)
                                                               ggctggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGAtcatca                           >  1:4453783/1‑93 (MQ=255)
                                                                 ctggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATg                         <  1:7372821/93‑1 (MQ=255)
                                                                  tggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGa                        <  1:5621081/93‑1 (MQ=255)
                                                                  tggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGa                        >  1:9618665/1‑93 (MQ=255)
                                                                  tggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGa                        >  2:4285574/1‑93 (MQ=255)
                                                                  tggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGa                        >  1:7927917/1‑93 (MQ=255)
                                                                   ggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACTTCGTTTTTTGCCCGAATAAGGAAGTGTACGATGACGAGGAGGATTATCATGAg                       >  1:8945090/1‑93 (MQ=255)
                                                                   ggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAg                       >  1:8562337/1‑93 (MQ=255)
                                                                    gctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGt                      <  1:5402537/93‑1 (MQ=255)
                                                                      tttATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTaa                    <  2:6386117/93‑1 (MQ=255)
                                                                       ttATTGCCACTTTTGTCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAg                   >  1:1059581/1‑93 (MQ=255)
                                                                       ttATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAg                   <  2:2875936/93‑1 (MQ=255)
                                                                       ttATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAg                   <  1:2607429/93‑1 (MQ=255)
                                                                       ttATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAg                   >  1:5002083/1‑93 (MQ=255)
                                                                        tATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGt                  <  2:7548317/93‑1 (MQ=255)
                                                                        tATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGt                  <  1:884337/93‑1 (MQ=255)
                                                                          ttGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCg                <  1:10017829/93‑1 (MQ=255)
                                                                          ttGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCg                >  2:9483548/1‑93 (MQ=255)
                                                                          ttGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCg                >  1:6747055/1‑93 (MQ=255)
                                                                          ttGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCg                <  2:6197107/93‑1 (MQ=255)
                                                                          ttGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGAAAGTCg                >  1:8392402/1‑93 (MQ=255)
                                                                           tGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGa               <  2:6165910/93‑1 (MQ=255)
                                                                             ccACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGaaa             <  2:8641857/93‑1 (MQ=255)
                                                                              cACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGaaaa            <  2:3960910/93‑1 (MQ=255)
                                                                                 ttttGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGa         <  2:6502950/93‑1 (MQ=255)
                                                                                 ttttGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGa         <  1:4854634/93‑1 (MQ=255)
                                                                                   ttGCCGGGCTGATGTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGAtt       <  2:2211911/93‑1 (MQ=255)
                                                                                    tGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATtg      <  2:835117/93‑1 (MQ=255)
                                                                                    tGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATtg      <  1:1240018/93‑1 (MQ=255)
                                                                                    tGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATtg      <  2:3271370/93‑1 (MQ=255)
                                                                                     gCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATtgt     <  2:3579828/93‑1 (MQ=255)
                                                                                     gCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATtgt     <  2:9677932/93‑1 (MQ=255)
                                                                                       cGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTgc   <  2:667772/93‑1 (MQ=255)
                                                                                        gggTTCATTTTCCACTACATCGGTATTGGCCCGAAGAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTgcg  <  1:3817441/93‑1 (MQ=255)
                                                                                        gggCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTgcg  >  2:1704813/1‑93 (MQ=255)
                                                                                           |                                                                                         
GAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCG  >  NC_000913/1549207‑1549387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCA  >  NC_000913/1549206‑1549389
                                                                                            |                                                                                           
CGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTT                                                                                            >  SRR4302540.2188406/1‑94 (MQ=60)
CGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCT                                                                                            >  SRR4302540.1159985/1‑94 (MQ=60)
CGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCT                                                                                            <  SRR4302540.4599908/94‑1 (MQ=60)
 GAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTG                                                                                           <  SRR4302540.4617605/94‑1 (MQ=60)
  AAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGA                                                                                          >  SRR4302540.6717064/1‑94 (MQ=60)
   AAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGAT                                                                                         >  SRR4302540.1178173/1‑94 (MQ=60)
     GATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTT                                                                                       >  SRR4302540.3342004/1‑94 (MQ=60)
      ATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTT                                                                                      <  SRR4302540.8477176/94‑1 (MQ=60)
      ATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTT                                                                                      >  SRR4302540.62471/1‑94 (MQ=60)
       TCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTC                                                                                     <  SRR4302540.9984606/94‑1 (MQ=60)
       TCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTC                                                                                     >  SRR4302540.3464876/1‑94 (MQ=60)
         CGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCA                                                                                   >  SRR4302540.3023017/1‑94 (MQ=60)
         CGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCA                                                                                   <  SRR4302540.3999818/94‑1 (MQ=60)
          GAAGATCGACCCCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCAC                                                                                  <  SRR4302540.2257767/94‑1 (MQ=60)
          GAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCGGGGCTGATTTTCCAC                                                                                  <  SRR4302540.3993967/94‑1 (MQ=60)
          GAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCAC                                                                                  >  SRR4302540.6930567/1‑94 (MQ=60)
          GAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCAC                                                                                  >  SRR4302540.9316968/1‑94 (MQ=60)
              ATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACA                                                                              >  SRR4302540.4762847/1‑94 (MQ=60)
               TCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACAT                                                                             <  SRR4302540.3438842/94‑1 (MQ=60)
               TCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACAT                                                                             <  SRR4302540.6184494/94‑1 (MQ=60)
               TCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACAT                                                                             <  SRR4302540.8138480/94‑1 (MQ=60)
                 GACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCG                                                                           >  SRR4302540.1438854/1‑94 (MQ=60)
                  ACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGG                                                                          <  SRR4302540.5401226/94‑1 (MQ=60)
                   CACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGT                                                                         >  SRR4302540.3758676/1‑94 (MQ=60)
                   CACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGT                                                                         <  SRR4302540.2154225/94‑1 (MQ=60)
                     CCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTAT                                                                       <  SRR4302540.10101718/94‑1 (MQ=60)
                       TCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTG                                                                     <  SRR4302540.3130811/94‑1 (MQ=60)
                          GTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCC                                                                  <  SRR4302540.149237/94‑1 (MQ=60)
                           TAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCC                                                                 >  SRR4302540.5298308/1‑94 (MQ=60)
                           TAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCC                                                                 <  SRR4302540.9503758/94‑1 (MQ=60)
                            AAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCG                                                                >  SRR4302540.9913589/1‑94 (MQ=60)
                             AGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGA                                                               <  SRR4302540.3284375/94‑1 (MQ=60)
                              GCCTGTGGAAAGGCGCGTTGAAACAGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTTCACTACCTCGGTATTGTGCCGAA                                                              >  SRR4302540.4265520/1‑94 (MQ=60)
                               CCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAT                                                             <  SRR4302540.1522950/94‑1 (MQ=60)
                               CCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAT                                                             <  SRR4302540.6836074/94‑1 (MQ=60)
                               CCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAT                                                             <  SRR4302540.4325875/94‑1 (MQ=60)
                               CCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAA                                                             >  SRR4302540.8124812/1‑94 (MQ=60)
                                CTTTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATA                                                            <  SRR4302540.2666392/94‑1 (MQ=60)
                                CTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGCATA                                                            >  SRR4302540.1872595/1‑94 (MQ=60)
                                CTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATA                                                            >  SRR4302540.5820904/1‑94 (MQ=60)
                                CTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATA                                                            <  SRR4302540.2098328/94‑1 (MQ=60)
                                 TGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAA                                                           >  SRR4302540.757427/1‑94 (MQ=60)
                                 TGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTACACTACATCGGTATTGGCCCGAATAA                                                           >  SRR4302540.8343561/1‑94 (MQ=60)
                                  GTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGCTTTTCAACTACATCGGTATTGGCCCGAATAAG                                                          >  SRR4302540.5883251/1‑94 (MQ=60)
                                  GTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAG                                                          >  SRR4302540.5883252/1‑94 (MQ=60)
                                  GTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAG                                                          >  SRR4302540.1149180/1‑94 (MQ=60)
                                     GAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAA                                                       >  SRR4302540.4704726/1‑94 (MQ=60)
                                      AAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAG                                                      <  SRR4302540.3931635/94‑1 (MQ=60)
                                      AAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCGTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAG                                                      <  SRR4302540.9805006/94‑1 (MQ=60)
                                         GGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGG                                                   <  SRR4302540.3039339/94‑1 (MQ=60)
                                            GCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACG                                                >  SRR4302540.8472459/1‑94 (MQ=60)
                                            GCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACG                                                <  SRR4302540.4893150/94‑1 (MQ=60)
                                              GTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGAT                                              >  SRR4302540.5865194/1‑94 (MQ=60)
                                              GTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGAT                                              <  SRR4302540.6536142/94‑1 (MQ=60)
                                               TTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATG                                             <  SRR4302540.5690042/94‑1 (MQ=60)
                                                TGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGA                                            <  SRR4302540.373969/94‑1 (MQ=60)
                                                 GAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGAC                                           <  SRR4302540.429882/94‑1 (MQ=60)
                                                 GAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGAC                                           <  SRR4302540.9243108/94‑1 (MQ=60)
                                                 GAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGAC                                           >  SRR4302540.9615815/1‑94 (MQ=60)
                                                  AAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACG                                          >  SRR4302540.3809523/1‑94 (MQ=60)
                                                  AAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACG                                          >  SRR4302540.3809821/1‑94 (MQ=60)
                                                    ACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAG                                        >  SRR4302540.4051546/1‑94 (MQ=60)
                                                    ACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAG                                        >  SRR4302540.5783406/1‑94 (MQ=60)
                                                     CCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGG                                       >  SRR4302540.9923742/1‑94 (MQ=60)
                                                      CGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGA                                      <  SRR4302540.1617038/94‑1 (MQ=60)
                                                      CGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGA                                      <  SRR4302540.8276334/94‑1 (MQ=60)
                                                       GCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAG                                     <  SRR4302540.5245924/94‑1 (MQ=60)
                                                       GCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAG                                     <  SRR4302540.9618406/94‑1 (MQ=60)
                                                       GCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCAGCATAAGGAAGAGGAAGCTGACGAGGAG                                     >  SRR4302540.2639195/1‑94 (MQ=60)
                                                        CTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGG                                    <  SRR4302540.9300730/94‑1 (MQ=60)
                                                        CTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTGCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGG                                    >  SRR4302540.5540774/1‑94 (MQ=60)
                                                          GGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGAT                                  <  SRR4302540.3968941/94‑1 (MQ=60)
                                                           GCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATC                                 <  SRR4302540.4044946/94‑1 (MQ=60)
                                                           GCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATC                                 >  SRR4302540.1794320/1‑94 (MQ=60)
                                                             AGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAATGGACGATGACGAGGAGGCTCAT                               >  SRR4302540.6669175/1‑94 (MQ=60)
                                                              GCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATC                              <  SRR4302540.9410746/94‑1 (MQ=60)
                                                              GCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATC                              >  SRR4302540.3756831/1‑94 (MQ=60)
                                                               CGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCA                             >  SRR4302540.870995/1‑94 (MQ=60)
                                                               CGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCA                             >  SRR4302540.4502062/1‑94 (MQ=60)
                                                                  CTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGA                          >  SRR4302540.9732054/1‑94 (MQ=60)
                                                                  CTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGA                          >  SRR4302540.8020315/1‑94 (MQ=60)
                                                                  CTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGA                          <  SRR4302540.7455001/94‑1 (MQ=60)
                                                                   TGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACTTCGTTTTTTGCCCGAATAAGGAAGTGTACGATGACGAGGAGGATTATCATGAG                         >  SRR4302540.9050277/1‑94 (MQ=60)
                                                                   TGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAG                         <  SRR4302540.5682925/94‑1 (MQ=60)
                                                                   TGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAG                         >  SRR4302540.8661776/1‑94 (MQ=60)
                                                                   TGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAG                         >  SRR4302540.7055853/1‑94 (MQ=60)
                                                                   TGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAG                         >  SRR4302540.4020802/1‑94 (MQ=60)
                                                                    GGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGT                        <  SRR4302540.4543185/94‑1 (MQ=60)
                                                                     GCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTA                       <  SRR4302540.5461934/94‑1 (MQ=60)
                                                                       TTTATTGCCACTTTTGTCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAG                     >  SRR4302540.1070366/1‑94 (MQ=60)
                                                                       TTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAG                     <  SRR4302540.271393/94‑1 (MQ=60)
                                                                       TTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAG                     >  SRR4302540.5056251/1‑94 (MQ=60)
                                                                       TTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAG                     <  SRR4302540.838070/94‑1 (MQ=60)
                                                                        TTATTGCCACTTTTGCCGGGCTGGTTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGT                    >  SRR4302540.5178069/1‑94 (MQ=60)
                                                                        TTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGT                    <  SRR4302540.2635568/94‑1 (MQ=60)
                                                                        TTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGT                    <  SRR4302540.2635567/94‑1 (MQ=60)
                                                                        TTATTGCCACTTTTGCCGGGCTGATTTGCCACTGCATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGT                    <  SRR4302540.2635565/94‑1 (MQ=60)
                                                                         TATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTC                   <  SRR4302540.893717/94‑1 (MQ=60)
                                                                          ATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCG                  >  SRR4302540.7068271/1‑94 (MQ=60)
                                                                          ATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCG                  >  SRR4302540.6821607/1‑94 (MQ=60)
                                                                          ATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCG                  >  SRR4302540.9929614/1‑94 (MQ=60)
                                                                          ATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGCAAGTCG                  >  SRR4302540.8489798/1‑94 (MQ=60)
                                                                          ATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGAAAGTCG                  >  SRR4302540.8489797/1‑94 (MQ=60)
                                                                           TTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGA                 <  SRR4302540.10134430/94‑1 (MQ=60)
                                                                               CACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAAT             <  SRR4302540.1295914/94‑1 (MQ=60)
                                                                               CACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAAT             >  SRR4302540.1373192/1‑94 (MQ=60)
                                                                                ACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATG            >  SRR4302540.5206270/1‑94 (MQ=60)
                                                                                 CTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGA           >  SRR4302540.2147105/1‑94 (MQ=60)
                                                                                  TTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGAT          >  SRR4302540.857339/1‑94 (MQ=60)
                                                                                  TTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGAT          <  SRR4302540.4906674/94‑1 (MQ=60)
                                                                                   TTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATT         >  SRR4302540.4943082/1‑94 (MQ=60)
                                                                                    TTGCTGGGCTGATTTTCCACTACATCGGTAGTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTG        <  SRR4302540.8968707/94‑1 (MQ=60)
                                                                                     TGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGT       <  SRR4302540.5281515/94‑1 (MQ=60)
                                                                                     TGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGT       <  SRR4302540.1252680/94‑1 (MQ=60)
                                                                                     TGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGT       <  SRR4302540.1252679/94‑1 (MQ=60)
                                                                                       CCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGC     >  SRR4302540.103356/1‑94 (MQ=60)
                                                                                        CGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCG    >  SRR4302540.8111841/1‑94 (MQ=60)
                                                                                        CGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCG    >  SRR4302540.2053910/1‑94 (MQ=60)
                                                                                         GGGTTCATTTTCCACTACATCGGTATTGGCCCGAAGAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGC   <  SRR4302540.3859003/94‑1 (MQ=60)
                                                                                         GGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGC   >  SRR4302540.8932071/1‑94 (MQ=60)
                                                                                          GGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCA  >  SRR4302540.8029669/1‑94 (MQ=60)
                                                                                            |                                                                                           
CGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCA  >  NC_000913/1549206‑1549389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑