Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,549,298 T→C L272L (TTG→CTG)  fdnH → formate dehydrogenase‑N, nitrate‑inducible, iron‑sulfur beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,549,2980TC100.0% 668.9 / NA 208L272L (TTG→CTG) fdnHformate dehydrogenase‑N, nitrate‑inducible, iron‑sulfur beta subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (98/110);  total (98/110)

Only 100 of 214 total aligned reads displayed.

AAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCG  >  NC_000913/1549208‑1549387
                                                                                          |                                                                                         
  aGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGAt                                                                                       >  1:1978452/1‑93 (MQ=255)
     tCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGAtttt                                                                                    >  1:4614942/1‑93 (MQ=255)
              cGAGACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACAt                                                                           <  2:1187281/93‑1 (MQ=255)
              cGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACAt                                                                           <  1:5358808/93‑1 (MQ=255)
               gACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATc                                                                          <  1:2226917/93‑1 (MQ=255)
                acacCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTTCACTACATCg                                                                         >  2:1258961/1‑93 (MQ=255)
                acacCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCg                                                                         <  2:953889/93‑1 (MQ=255)
                acacCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCg                                                                         >  2:3016626/1‑93 (MQ=255)
                   ccTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTa                                                                      >  2:2600457/1‑93 (MQ=255)
                   ccTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTa                                                                      >  1:6197136/1‑93 (MQ=255)
                   ccTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTa                                                                      >  2:5129863/1‑93 (MQ=255)
                   ccTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTAATGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTa                                                                      >  2:2870005/1‑93 (MQ=255)
                    cTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGCTTTTCCACTACATCGGTAt                                                                     >  1:2236145/1‑93 (MQ=255)
                    cTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTAt                                                                     <  2:1569906/93‑1 (MQ=255)
                       ggTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTgg                                                                  >  1:5226536/1‑93 (MQ=255)
                       ggTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTgg                                                                  >  1:4165981/1‑93 (MQ=255)
                        gTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGc                                                                 <  1:3975912/93‑1 (MQ=255)
                         tAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGcc                                                                >  1:4688110/1‑93 (MQ=255)
                           aGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCg                                                              >  1:5984247/1‑93 (MQ=255)
                           aGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCg                                                              <  1:4865342/93‑1 (MQ=255)
                            gCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGa                                                             >  1:2319951/1‑93 (MQ=255)
                              cTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAt                                                           >  2:122924/1‑93 (MQ=255)
                              cTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAt                                                           >  2:5040573/1‑93 (MQ=255)
                              cTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAt                                                           >  1:846120/1‑93 (MQ=255)
                               tgtgGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATa                                                          >  2:36812/1‑93 (MQ=255)
                               tgtgGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATa                                                          >  1:5658505/1‑93 (MQ=255)
                                gtgGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATaa                                                         <  1:3026230/93‑1 (MQ=255)
                                  ggAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAgg                                                       >  2:3228774/1‑93 (MQ=255)
                                   gAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGa                                                      <  2:4009982/93‑1 (MQ=255)
                                   gAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGa                                                      >  2:2298089/1‑93 (MQ=255)
                                    aaaGGCGCGTTGAACCCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGaa                                                     <  2:4589225/93‑1 (MQ=255)
                                        gcgcGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTgg                                                 <  1:960547/93‑1 (MQ=255)
                                        gcgcGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTgg                                                 <  1:6228865/93‑1 (MQ=255)
                                          gcgTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGAc                                               >  1:147941/1‑93 (MQ=255)
                                            gTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGa                                             <  2:4219022/93‑1 (MQ=255)
                                            gTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTGGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGa                                             <  1:1641298/93‑1 (MQ=255)
                                             ttGAAACCGCTGGCAGCGGCTTGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGAt                                            >  1:781104/1‑93 (MQ=255)
                                             ttGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGAt                                            >  2:1634128/1‑93 (MQ=255)
                                             ttGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGAt                                            >  2:3350298/1‑93 (MQ=255)
                                              tGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATg                                           >  2:1347282/1‑93 (MQ=255)
                                               gAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGa                                          >  2:2357290/1‑93 (MQ=255)
                                               gAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGa                                          >  1:5421629/1‑93 (MQ=255)
                                               gAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGa                                          <  2:4035812/93‑1 (MQ=255)
                                               gAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGa                                          >  1:4650138/1‑93 (MQ=255)
                                                 aaCCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACg                                        <  1:3823581/93‑1 (MQ=255)
                                                 aaCCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACg                                        <  1:1536577/93‑1 (MQ=255)
                                                  aCCTCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGTGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACga                                       <  2:3961868/93‑1 (MQ=255)
                                                  aCCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACga                                       <  1:5330573/93‑1 (MQ=255)
                                                  aCCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACga                                       <  1:1354233/93‑1 (MQ=255)
                                                  aCCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACga                                       >  1:2174923/1‑93 (MQ=255)
                                                  aCCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACga                                       <  1:4232526/93‑1 (MQ=255)
                                                  aCCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACga                                       <  1:4341377/93‑1 (MQ=255)
                                                   ccGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACgag                                      >  1:5521231/1‑93 (MQ=255)
                                                   ccGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACgag                                      <  2:406827/93‑1 (MQ=255)
                                                   ccGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACgag                                      >  1:5926929/1‑93 (MQ=255)
                                                     gCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACgagga                                    <  1:3451401/93‑1 (MQ=255)
                                                       tGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGaggagg                                  >  1:499614/1‑93 (MQ=255)
                                                       tGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGaggagg                                  >  2:5782805/1‑93 (MQ=255)
                                                        ggCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACCATGACGAggagga                                 <  2:6193368/93‑1 (MQ=255)
                                                         gCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGat                                <  1:1081792/93‑1 (MQ=255)
                                                         gCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGat                                <  1:317060/93‑1 (MQ=255)
                                                          cAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGatc                               <  1:2558967/93‑1 (MQ=255)
                                                           aGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGatca                              >  1:2140294/1‑93 (MQ=255)
                                                             cGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGatcatc                            <  2:4317801/93‑1 (MQ=255)
                                                             cGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGatcatc                            <  2:273250/93‑1 (MQ=255)
                                                              gtctggctTGATTGCCACTTTTGCCGGTCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGAtcatca                           <  1:5759251/91‑1 (MQ=255)
                                                              ggctggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGAtcatca                           >  1:6283521/1‑93 (MQ=255)
                                                              ggctggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGAtcatca                           <  1:1435889/93‑1 (MQ=255)
                                                              ggctggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGAtcatca                           <  1:3899540/93‑1 (MQ=255)
                                                               gctggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATcatcat                          >  2:4379365/1‑93 (MQ=255)
                                                               gctggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATcatcat                          >  2:65227/1‑93 (MQ=255)
                                                                  ggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAg                       <  1:611849/93‑1 (MQ=255)
                                                                  ggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAg                       >  1:1097935/1‑93 (MQ=255)
                                                                  ggctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAg                       >  1:449087/1‑93 (MQ=255)
                                                                   gctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGt                      <  2:4497176/93‑1 (MQ=255)
                                                                   gctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATAATCATGAga                      >  1:5053615/1‑92 (MQ=255)
                                                                    ctTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTa                     <  1:3207956/93‑1 (MQ=255)
                                                                     tttATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTaa                    <  2:101218/93‑1 (MQ=255)
                                                                     tttATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTaa                    <  1:2706785/93‑1 (MQ=255)
                                                                     tttATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTaa                    >  1:3058366/1‑93 (MQ=255)
                                                                     tttATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTaa                    >  2:5592099/1‑93 (MQ=255)
                                                                       tATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGt                  <  2:5337443/93‑1 (MQ=255)
                                                                         ttGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCg                <  1:5729426/93‑1 (MQ=255)
                                                                         ttGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCg                >  1:1152141/1‑93 (MQ=255)
                                                                          tGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGa               <  2:6227715/93‑1 (MQ=255)
                                                                           gCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCgca              >  1:5250860/1‑91 (MQ=255)
                                                                            ccACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGaaa             <  2:1519366/93‑1 (MQ=255)
                                                                             cACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGaaaa            >  1:2761547/1‑93 (MQ=255)
                                                                             cACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGaaaa            >  2:5506764/1‑93 (MQ=255)
                                                                               cTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACTATGACGAGGAGGATCATCATGAGTAAGTCGAAAATg          <  1:3414255/93‑1 (MQ=255)
                                                                               cTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATg          >  1:4745805/1‑93 (MQ=255)
                                                                                ttttGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGa         >  1:5548018/1‑93 (MQ=255)
                                                                                 tttGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGAt        <  2:3297652/93‑1 (MQ=255)
                                                                                 tttGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGAt        <  2:917573/93‑1 (MQ=255)
                                                                                   tGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATtg      <  2:1386811/93‑1 (MQ=255)
                                                                                    gCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATtgt     <  1:2942986/93‑1 (MQ=255)
                                                                                    gCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATtgt     <  2:5973743/93‑1 (MQ=255)
                                                                                     ccGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATtgtg    <  2:1933010/93‑1 (MQ=255)
                                                                                      cGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTgc   <  2:684990/93‑1 (MQ=255)
                                                                                      cGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTgc   <  2:4132991/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCG  >  NC_000913/1549208‑1549387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCACC  >  NC_000913/1549205‑1549391
                                                                                             |                                                                                             
CCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGC                                                                                               >  SRR4302533.4659138/1‑94 (MQ=60)
  GAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTG                                                                                             >  SRR4302533.2640448/1‑94 (MQ=60)
   AAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGA                                                                                            <  SRR4302533.6056167/94‑1 (MQ=60)
    AAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGAT                                                                                           >  SRR4302533.1990569/1‑94 (MQ=60)
       ATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTT                                                                                        >  SRR4302533.4642893/1‑94 (MQ=60)
          CGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCA                                                                                     >  SRR4302533.532854/1‑94 (MQ=60)
           GAAGATCGACACCTCGGTAAGCCTGTGGAAAGGAGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCAC                                                                                    >  SRR4302533.2190523/1‑94 (MQ=60)
             AGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCacctc                                                                                  >  SRR4302533.1930415/1‑89 (MQ=60)
              GATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTAC                                                                                 <  SRR4302533.524325/94‑1 (MQ=60)
              GATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTAC                                                                                 <  SRR4302533.4276530/94‑1 (MQ=60)
               ATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACA                                                                                >  SRR4302533.3306626/1‑94 (MQ=60)
                 CGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATC                                                                              <  SRR4302533.5390976/94‑1 (MQ=60)
                  GACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCG                                                                             >  SRR4302533.5273789/1‑94 (MQ=60)
                  GACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCG                                                                             <  SRR4302533.2240664/94‑1 (MQ=60)
                   ACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGG                                                                            <  SRR4302533.4655321/94‑1 (MQ=60)
                   ACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCAGGCTGATTTTCCACTACATCGG                                                                            >  SRR4302533.6146944/1‑94 (MQ=60)
                    CACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGT                                                                           >  SRR4302533.3039923/1‑94 (MQ=60)
                     ACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTA                                                                          >  SRR4302533.3909908/1‑94 (MQ=60)
                     ACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTA                                                                          >  SRR4302533.6234315/1‑94 (MQ=60)
                      CCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGCTTTTCCACTACATCGGTAT                                                                         >  SRR4302533.2249970/1‑94 (MQ=60)
                      CCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTAT                                                                         >  SRR4302533.2167401/1‑94 (MQ=60)
                      CCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTAT                                                                         <  SRR4302533.46174/94‑1 (MQ=60)
                       CTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATT                                                                        >  SRR4302533.803161/1‑94 (MQ=60)
                         CGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGG                                                                      >  SRR4302533.5257986/1‑94 (MQ=60)
                         CGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGG                                                                      >  SRR4302533.4191225/1‑94 (MQ=60)
                          GGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGC                                                                     <  SRR4302533.6012760/94‑1 (MQ=60)
                           GTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCC                                                                    >  SRR4302533.4716526/1‑94 (MQ=60)
                           GTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCC                                                                    <  SRR4302533.4000115/94‑1 (MQ=60)
                            TAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCC                                                                   >  SRR4302533.6219200/1‑94 (MQ=60)
                             AAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCG                                                                  >  SRR4302533.6020489/1‑94 (MQ=60)
                              AGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGA                                                                 <  SRR4302533.4894826/94‑1 (MQ=60)
                              AGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGA                                                                 >  SRR4302533.2334316/1‑94 (MQ=60)
                                CCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAT                                                               >  SRR4302533.851072/1‑94 (MQ=60)
                                CCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAAT                                                               <  SRR4302533.2731883/94‑1 (MQ=60)
                                 CTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATA                                                              >  SRR4302533.4306097/1‑94 (MQ=60)
                                 CTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATA                                                              >  SRR4302533.5692697/1‑94 (MQ=60)
                                  TGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAA                                                             <  SRR4302533.983512/94‑1 (MQ=60)
                                  TGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAA                                                             >  SRR4302533.5571722/1‑94 (MQ=60)
                                   GTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAG                                                            <  SRR4302533.3044748/94‑1 (MQ=60)
                                      GAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAA                                                         <  SRR4302533.926902/94‑1 (MQ=60)
                                          GGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGG                                                     <  SRR4302533.1653526/94‑1 (MQ=60)
                                           GCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGA                                                    <  SRR4302533.966192/94‑1 (MQ=60)
                                           GCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGA                                                    <  SRR4302533.6266176/94‑1 (MQ=60)
                                            CGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGAC                                                   >  SRR4302533.148888/1‑94 (MQ=60)
                                             GCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACG                                                  <  SRR4302533.3946933/94‑1 (MQ=60)
                                               GTTGAAACCGCTGGCAGCGGCTTGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGAT                                                >  SRR4302533.785704/1‑94 (MQ=60)
                                               GTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGAT                                                >  SRR4302533.4775485/1‑94 (MQ=60)
                                               GTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTGGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGAT                                                <  SRR4302533.1651241/94‑1 (MQ=60)
                                                 TGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGA                                              >  SRR4302533.5454252/1‑94 (MQ=60)
                                                 TGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGA                                              >  SRR4302533.4678314/1‑94 (MQ=60)
                                                    AACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGA                                           <  SRR4302533.1577405/94‑1 (MQ=60)
                                                    AACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGA                                           <  SRR4302533.1545808/94‑1 (MQ=60)
                                                    AACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGA                                           <  SRR4302533.3846958/94‑1 (MQ=60)
                                                    AACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGA                                           >  SRR4302533.2188326/1‑94 (MQ=60)
                                                     ACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTTGCCCGAATAAGGAAGTGGACGATGACGAG                                          <  SRR4302533.2686123/94‑1 (MQ=60)
                                                     ACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAG                                          <  SRR4302533.4367541/94‑1 (MQ=60)
                                                     ACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAG                                          >  SRR4302533.5962758/1‑94 (MQ=60)
                                                     ACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAG                                          >  SRR4302533.5554565/1‑94 (MQ=60)
                                                     ACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAG                                          <  SRR4302533.5362521/94‑1 (MQ=60)
                                                     ACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAG                                          <  SRR4302533.4258107/94‑1 (MQ=60)
                                                     ACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAG                                          <  SRR4302533.1362373/94‑1 (MQ=60)
                                                       CGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGG                                        >  SRR4302533.1293064/1‑94 (MQ=60)
                                                        GCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAG                                       >  SRR4302533.2416930/1‑94 (MQ=60)
                                                        GCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAG                                       <  SRR4302533.3472711/94‑1 (MQ=60)
                                                         CTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGG                                      >  SRR4302533.502589/1‑94 (MQ=60)
                                                          TGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGA                                     >  SRR4302533.3463380/1‑94 (MQ=60)
                                                            GCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATC                                   <  SRR4302533.1088174/94‑1 (MQ=60)
                                                            GCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATC                                   <  SRR4302533.319001/94‑1 (MQ=60)
                                                            GCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATC                                   <  SRR4302533.1502703/94‑1 (MQ=60)
                                                            GCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATC                                   <  SRR4302533.5786602/94‑1 (MQ=60)
                                                             CAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCA                                  >  SRR4302533.2153488/1‑94 (MQ=60)
                                                             CAGCGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCA                                  <  SRR4302533.2574701/94‑1 (MQ=60)
                                                                CGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCA                               >  SRR4302533.6321016/1‑94 (MQ=60)
                                                                CGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCA                               >  SRR4302533.4946567/1‑94 (MQ=60)
                                                                CGGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCA                               >  SRR4302533.307670/1‑94 (MQ=60)
                                                                 GTCTGGCTTGATTGCCACTTTTGCCGGTCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCAT                              <  SRR4302533.5794004/94‑1 (MQ=60)
                                                                 GGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCAT                              <  SRR4302533.1444557/94‑1 (MQ=60)
                                                                 GGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCAT                              >  SRR4302533.4951933/1‑94 (MQ=60)
                                                                 GGCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCAT                              <  SRR4302533.3923336/94‑1 (MQ=60)
                                                                  GCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATG                             >  SRR4302533.1458439/1‑94 (MQ=60)
                                                                  GCTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATG                             <  SRR4302533.3322510/94‑1 (MQ=60)
                                                                   CTGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGA                            >  SRR4302533.449127/1‑94 (MQ=60)
                                                                    TGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAG                           >  SRR4302533.5071550/1‑94 (MQ=60)
                                                                    TGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAG                           >  SRR4302533.1104437/1‑94 (MQ=60)
                                                                    TGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAG                           <  SRR4302533.3142301/94‑1 (MQ=60)
                                                                    TGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAG                           >  SRR4302533.2111485/1‑94 (MQ=60)
                                                                    TGGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAG                           >  SRR4302533.451773/1‑94 (MQ=60)
                                                                     GGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGT                          <  SRR4302533.615446/94‑1 (MQ=60)
                                                                     GGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGT                          <  SRR4302533.5657917/94‑1 (MQ=60)
                                                                     GGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGT                          <  SRR4302533.4724542/94‑1 (MQ=60)
                                                                     GGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGT                          >  SRR4302533.2926380/1‑94 (MQ=60)
                                                                     GGCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATAATCATGAGA                          >  SRR4302533.5084167/1‑94 (MQ=60)
                                                                      GCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTA                         <  SRR4302533.2249880/94‑1 (MQ=60)
                                                                      GCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTA                         <  SRR4302533.1437350/94‑1 (MQ=60)
                                                                      GCTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTA                         <  SRR4302533.6289965/94‑1 (MQ=60)
                                                                       GTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAA                        <  SRR4302533.5507925/94‑1 (MQ=60)
                                                                       CTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAA                        <  SRR4302533.5510013/94‑1 (MQ=60)
                                                                       CTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAA                        >  SRR4302533.3077134/1‑94 (MQ=60)
                                                                       CTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAA                        <  SRR4302533.3227710/94‑1 (MQ=60)
                                                                       CTTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAA                        <  SRR4302533.3547695/94‑1 (MQ=60)
                                                                        TTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAG                       <  SRR4302533.2723391/94‑1 (MQ=60)
                                                                        TTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAG                       <  SRR4302533.3203574/94‑1 (MQ=60)
                                                                        TTTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAG                       <  SRR4302533.1097901/94‑1 (MQ=60)
                                                                         TTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGT                      >  SRR4302533.2267403/1‑94 (MQ=60)
                                                                         TTATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGT                      >  SRR4302533.1140384/1‑94 (MQ=60)
                                                                          TATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTC                     <  SRR4302533.766107/94‑1 (MQ=60)
                                                                           ATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCG                    >  SRR4302533.1159041/1‑94 (MQ=60)
                                                                           ATTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCG                    <  SRR4302533.5363852/94‑1 (MQ=60)
                                                                            TTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGA                   <  SRR4302533.5764023/94‑1 (MQ=60)
                                                                            TTGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGA                   <  SRR4302533.4695016/94‑1 (MQ=60)
                                                                             TGCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGCA                  >  SRR4302533.5282413/1‑94 (MQ=60)
                                                                              GCCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAA                 >  SRR4302533.6250736/1‑94 (MQ=60)
                                                                               CCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAA                <  SRR4302533.1681932/94‑1 (MQ=60)
                                                                               CCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAA                >  SRR4302533.5721643/1‑94 (MQ=60)
                                                                               CCACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAA                >  SRR4302533.2778473/1‑94 (MQ=60)
                                                                                 ACTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATG              >  SRR4302533.4774578/1‑94 (MQ=60)
                                                                                  CTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACTATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGA             <  SRR4302533.3435349/94‑1 (MQ=60)
                                                                                  CTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGA             <  SRR4302533.5829314/94‑1 (MQ=60)
                                                                                  CTTTTGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGA             >  SRR4302533.5581523/1‑94 (MQ=60)
                                                                                      TGCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGT         <  SRR4302533.4121862/94‑1 (MQ=60)
                                                                                       GCCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTG        <  SRR4302533.2960952/94‑1 (MQ=60)
                                                                                        CCGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGC       >  SRR4302533.543836/1‑94 (MQ=60)
                                                                                         CGGGCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGCAAGTCGAAAATGATTGTGCG      >  SRR4302533.4240176/1‑94 (MQ=60)
                                                                                            GCTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCAC   >  SRR4302533.3838351/1‑94 (MQ=60)
                                                                                             CTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCACC  >  SRR4302533.4077560/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTGGCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCACC  >  NC_000913/1549205‑1549391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑