Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,572,526	T→C	D234G (GAC→GGC)	pqqL ←	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,572,526	0	T	C	99.3%	911.7 / ‑4.2	282	D234G (GAC→GGC)	pqqL	putative peptidase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (143/137); total (144/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 293 total aligned reads displayed.

GCTTTTGTCGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGTCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCG  >  NC_000913/1572436‑1572615

gCTTTTGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCg                                                                                         >  1:9744692/1‑93 (MQ=255)

  ttttGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGat                                                                                       >  2:4445754/1‑93 (MQ=255)

   tttGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGatt                                                                                      >  2:8724938/1‑92 (MQ=255)

     tGTCGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCTGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATc                                                                                    <  2:4917848/93‑1 (MQ=255)

      gttggCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCg                                                                                   >  1:1520480/4‑93 (MQ=255)

       ttggCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGc                                                                                  <  2:2744827/91‑1 (MQ=255)

       ttggCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGc                                                                                  <  2:7682956/91‑1 (MQ=255)

        tggCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGcc                                                                                 <  1:9455377/92‑1 (MQ=255)

        tggCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGcc                                                                                 <  1:6832709/92‑1 (MQ=255)

         ggCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCg                                                                                >  1:3882078/1‑93 (MQ=255)

           ccAGACGTGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGAc                                                                              <  1:4124561/93‑1 (MQ=255)

            cAGNCGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGAcc                                                                             <  2:7668408/93‑1 (MQ=255)

            cAGACGCGATTTTCAGCAGATTTGTAAGCCGGAAGCTTACTTAAATTCTCCTTTATCAGCGCCAGCGCTTCTTTAATGCCGATATCGCCGAcc                                                                             <  2:8985325/93‑1 (MQ=255)

             aGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCa                                                                            >  1:7898435/1‑93 (MQ=255)

               aCGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACg                                                                          >  2:8269597/1‑93 (MQ=255)

                cgcgATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGa                                                                         >  1:9203045/1‑93 (MQ=255)

                cgcgATTGTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGa                                                                         <  2:1873561/93‑1 (MQ=255)

                    gttttCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATaaa                                                                     <  1:6690240/92‑1 (MQ=255)

                    aTTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATaaa                                                                     >  1:3311709/1‑93 (MQ=255)

                    aTTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATaaa                                                                     >  1:8558829/1‑93 (MQ=255)

                      tttCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAgg                                                                   <  2:1548146/93‑1 (MQ=255)

                       ttCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGt                                                                  >  1:4775594/1‑93 (MQ=255)

                        tCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTc                                                                 <  1:5247522/93‑1 (MQ=255)

                          aGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCat                                                               <  2:3215880/93‑1 (MQ=255)

                           gctgctTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCata                                                              <  1:4913516/93‑1 (MQ=255)

                            ctgctTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCatat                                                             <  2:5715754/93‑1 (MQ=255)

                            ctgctTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCatat                                                             <  2:4137638/93‑1 (MQ=255)

                             ttctTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCAtatt                                                            <  1:7198281/91‑1 (MQ=255)

                               ctTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCAtattat                                                          >  2:752614/1‑93 (MQ=255)

                                tttGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATattatt                                                         >  2:2175736/1‑93 (MQ=255)

                                 ttGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTAttt                                                        >  1:5367153/1‑93 (MQ=255)

                                 ttGTGAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTAttt                                                        <  1:1690231/93‑1 (MQ=255)

                                   gTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGAGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTgg                                                      >  2:2589333/1‑93 (MQ=255)

                                    ttAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGt                                                     <  2:1969904/93‑1 (MQ=255)

                                     tAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGtt                                                    >  2:2283598/1‑93 (MQ=255)

                                     tAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGtt                                                    <  2:7429261/93‑1 (MQ=255)

                                     tAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGtt                                                    <  1:179221/93‑1 (MQ=255)

                                      aGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTg                                                   >  1:2730363/1‑93 (MQ=255)

                                       gCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGGTAAAGGTCATAGTATTTGGTTGa                                                  >  2:6932400/1‑93 (MQ=255)

                                         cGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATa                                                >  1:6004548/1‑93 (MQ=255)

                                         cGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATa                                                <  1:8229690/93‑1 (MQ=255)

                                         cGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATa                                                >  2:42963/1‑93 (MQ=255)

                                         cGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATa                                                >  2:3506538/1‑93 (MQ=255)

                                           gAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATAcc                                              >  1:5080684/1‑93 (MQ=255)

                                           gAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATAcc                                              >  2:6430402/1‑93 (MQ=255)

                                           gAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATAcc                                              <  1:874573/93‑1 (MQ=255)

                                            aaGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCa                                             <  2:3774501/93‑1 (MQ=255)

                                            aaGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCa                                             <  2:9596702/93‑1 (MQ=255)

                                            aaGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCa                                             >  1:6310000/1‑93 (MQ=255)

                                              gCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGc                                           >  1:9304927/1‑93 (MQ=255)

                                              gCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGc                                           <  2:10042573/93‑1 (MQ=255)

                                              gCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGc                                           >  2:53532/1‑93 (MQ=255)

                                               cttacttaAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTTCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGct                                          >  1:5666837/1‑92 (MQ=255)

                                                ttacttaAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGt                                         >  2:9305567/1‑93 (MQ=255)

                                                ttacttaAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGt                                         <  2:7887554/93‑1 (MQ=255)

                                                 tacttaAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGtt                                        <  1:4546024/93‑1 (MQ=255)

                                                 tacttaAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGtt                                        >  1:2260981/1‑93 (MQ=255)

                                                 tacttaAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGtt                                        >  1:4261094/1‑93 (MQ=255)

                                                 tacttaAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGCCATATTATTTGGTTGATACCAGCGtt                                        >  1:9635017/1‑93 (MQ=255)

                                                  tcttaAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTg                                       <  2:9215725/92‑1 (MQ=255)

                                                   cttaAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGa                                      >  1:7412675/1‑93 (MQ=255)

                                                   cttaAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGa                                      <  1:9077268/93‑1 (MQ=255)

                                                         ttATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAAt                                >  1:6065461/1‑93 (MQ=255)

                                                         ttATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAAt                                <  2:2436267/93‑1 (MQ=255)

                                                          tATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAAtt                               >  2:4031333/1‑93 (MQ=255)

                                                           aTCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTg                              <  2:9701958/93‑1 (MQ=255)

                                                            tCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTgg                             >  1:8787864/1‑93 (MQ=255)

                                                            tCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTgg                             <  2:3301604/93‑1 (MQ=255)

                                                            tCCTTTATCAGCGCCAGCGCTTCTTTACAGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTgt                             >  1:1117968/1‑92 (MQ=255)

                                                              cTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGgcg                           >  1:5124322/1‑93 (MQ=255)

                                                              cTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGgcg                           >  1:2221562/1‑93 (MQ=255)

                                                                ttATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCa                         >  2:8151331/1‑93 (MQ=255)

                                                                  aTCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGAGCAAt                       >  2:1470479/1‑93 (MQ=255)

                                                                   tCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAAtt                      >  1:7491599/1‑93 (MQ=255)

                                                                   acatcgccagcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAAtt                      <  2:8520509/89‑1 (MQ=255)

                                                                     agcgccagcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATtgt                    <  2:619562/93‑1 (MQ=255)

                                                                     agcgccagcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATtgt                    >  2:6073168/1‑93 (MQ=255)

                                                                     agcgccagcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATtgt                    <  2:2333199/93‑1 (MQ=255)

                                                                      gcgccagcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATtgtg                   >  2:923293/1‑93 (MQ=255)

                                                                      gcgccagcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATtgtg                   <  1:2591374/93‑1 (MQ=255)

                                                                        gccagcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGcg                 >  2:2881729/1‑92 (MQ=255)

                                                                        gccagcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGcc                 <  2:1153631/93‑1 (MQ=255)

                                                                        gccagcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGcc                 >  1:775147/1‑93 (MQ=255)

                                                                         ccagcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCg                <  2:1591817/93‑1 (MQ=255)

                                                                         acagcgcTTCTTTACTGTCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTACCg                <  2:6828931/92‑1 (MQ=255)

                                                                           agcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGgt              >  2:8549423/1‑93 (MQ=255)

                                                                           agcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGgt              >  2:7316817/1‑93 (MQ=255)

                                                                           agcgcTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGgt              <  2:461486/93‑1 (MQ=255)

                                                                            gcgcTTCTTTACTGCCGATATCGCCGACAACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGgtg             <  1:1025897/93‑1 (MQ=255)

                                                                                ttcttTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGAc         >  1:8406813/1‑93 (MQ=255)

                                                                                ttcttTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGAc         >  1:3494932/1‑93 (MQ=255)

                                                                                 tcttTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGAcc        <  2:509553/93‑1 (MQ=255)

                                                                                 tcttTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGAcc        >  1:122232/1‑93 (MQ=255)

                                                                                   tttACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGt      <  1:849145/93‑1 (MQ=255)

                                                                                   tttACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGt      <  1:3089182/93‑1 (MQ=255)

                                                                                   tttACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGt      <  1:24785/93‑1 (MQ=255)

                                                                                   tttACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGt      <  2:267043/93‑1 (MQ=255)

                                                                                    ttACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTg     <  1:8018196/93‑1 (MQ=255)

                                                                                    ttACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTg     <  1:8542959/93‑1 (MQ=255)

                                                                                     tACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTgg    <  2:208153/93‑1 (MQ=255)

GCTTTTGTCGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGTCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCG  >  NC_000913/1572436‑1572615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGGCTTTTGTCGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGTCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCGACAG  >  NC_000913/1572433‑1572619

TCGGCTTTTGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGC                                                                                               >  SRR4302540.1985043/1‑94 (MQ=60)

 CGGCTTTTGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCC                                                                                              >  SRR4302540.971692/1‑94 (MQ=60)

 CGGCTTTTGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCC                                                                                              >  SRR4302540.9636899/1‑94 (MQ=60)

 CGGCTTTTGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCC                                                                                              <  SRR4302540.4216710/94‑1 (MQ=60)

  GGCTTTTGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCG                                                                                             >  SRR4302540.9859058/1‑94 (MQ=60)

  GGCTTTTGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTCTATCAGCGCCAGCGCTTCTTTACTGCCG                                                                                             >  SRR4302540.9858885/1‑94 (MQ=60)

  GGCTTTTGTCGGCCAGACGCGACTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCG                                                                                             >  SRR4302540.4095898/1‑94 (MQ=60)

    CTTTTGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGAT                                                                                           >  SRR4302540.2365964/1‑94 (MQ=60)

     TTTTGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATA                                                                                          >  SRR4302540.8262699/1‑94 (MQ=60)

      TTTGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATAT                                                                                         <  SRR4302540.9616732/94‑1 (MQ=60)

        TGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCG                                                                                       >  SRR4302540.1537312/1‑94 (MQ=60)

        TGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCG                                                                                       <  SRR4302540.2940584/94‑1 (MQ=60)

        TGTTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCG                                                                                       <  SRR4302540.9162403/94‑1 (MQ=60)

          TTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCC                                                                                     <  SRR4302540.1468626/94‑1 (MQ=60)

          TTGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCC                                                                                     >  SRR4302540.8614490/1‑94 (MQ=60)

           TGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCG                                                                                    >  SRR4302540.3924258/1‑94 (MQ=60)

           TGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCG                                                                                    <  SRR4302540.9567590/94‑1 (MQ=60)

           TGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCG                                                                                    <  SRR4302540.6908382/94‑1 (MQ=60)

           TGGCCAGAAGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCG                                                                                    <  SRR4302540.357697/94‑1 (MQ=60)

            GGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGA                                                                                   >  SRR4302540.9322694/1‑94 (MQ=60)

              CCAGACGTGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACC                                                                                 <  SRR4302540.4169111/94‑1 (MQ=60)

               CAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCA                                                                                >  SRR4302540.7990573/1‑94 (MQ=60)

                AGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCAC                                                                               <  SRR4302540.804124/94‑1 (MQ=60)

                AGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCAC                                                                               <  SRR4302540.804126/94‑1 (MQ=60)

                AGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCAC                                                                               >  SRR4302540.3410333/1‑94 (MQ=60)

                  ACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGA                                                                             >  SRR4302540.9313468/1‑94 (MQ=60)

                  ACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGA                                                                             >  SRR4302540.4594359/1‑94 (MQ=60)

                  ACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGA                                                                             >  SRR4302540.225107/1‑94 (MQ=60)

                   CGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGCT                                                                            >  SRR4302540.2675083/1‑94 (MQ=60)

                   CGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGAT                                                                            >  SRR4302540.9563534/1‑94 (MQ=60)

                   CGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGAT                                                                            <  SRR4302540.5022501/94‑1 (MQ=60)

                    GCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGTCGATATCGCCGACCACGATA                                                                           >  SRR4302540.6354050/1‑94 (MQ=60)

                     CGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAA                                                                          <  SRR4302540.7895045/94‑1 (MQ=60)

                      GATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGTCGATATCGCCGACCACGATAAA                                                                         <  SRR4302540.1613475/94‑1 (MQ=60)

                      GATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAA                                                                         >  SRR4302540.732763/1‑94 (MQ=60)

                      GATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAA                                                                         >  SRR4302540.3347995/1‑94 (MQ=60)

                      GATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAA                                                                         >  SRR4302540.8658236/1‑94 (MQ=60)

                      GATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAA                                                                         <  SRR4302540.418802/94‑1 (MQ=60)

                       GTTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAG                                                                        <  SRR4302540.6764105/94‑1 (MQ=60)

                         TTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGT                                                                      >  SRR4302540.4826616/1‑94 (MQ=60)

                          TTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTC                                                                     >  SRR4302540.1137242/1‑94 (MQ=60)

                           TCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCA                                                                    <  SRR4302540.5304755/94‑1 (MQ=60)

                           TCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCA                                                                    <  SRR4302540.9251555/94‑1 (MQ=60)

                           TCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCA                                                                    <  SRR4302540.3683827/94‑1 (MQ=60)

                             CGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATA                                                                  <  SRR4302540.7756020/94‑1 (MQ=60)

                             AGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATA                                                                  >  SRR4302540.831809/1‑94 (MQ=60)

                             AGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATA                                                                  <  SRR4302540.7756019/94‑1 (MQ=60)

                              GCTGCTTTTATAGCCGGAAGCTTTCTTAAATCATCCATGGTCAGCGCCAGCGCTTCTTTACGGCCGATATCGCCGACCTCGATAAAGGTCATAT                                                                 <  SRR4302540.4966425/94‑1 (MQ=42)

                              GCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATAT                                                                 <  SRR4302540.194620/94‑1 (MQ=60)

                              GCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATAT                                                                 <  SRR4302540.4966424/94‑1 (MQ=60)

                                TTCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTA                                                               <  SRR4302540.7278524/94‑1 (MQ=60)

                                 GCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTAT                                                              <  SRR4302540.5858941/94‑1 (MQ=60)

                                 GCTGTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTAT                                                              <  SRR4302540.4839124/94‑1 (MQ=60)

                                  CTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATT                                                             >  SRR4302540.7084365/1‑94 (MQ=60)

                                   TTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTT                                                            >  SRR4302540.5426129/1‑94 (MQ=60)

                                    TTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTG                                                           <  SRR4302540.8136352/94‑1 (MQ=60)

                                    TTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTG                                                           <  SRR4302540.1708563/94‑1 (MQ=60)

                                    TTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTG                                                           <  SRR4302540.3440653/94‑1 (MQ=60)

                                    TTGTGAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTG                                                           <  SRR4302540.1708562/94‑1 (MQ=60)

                                     TGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGG                                                          >  SRR4302540.8641535/1‑94 (MQ=60)

                                        TAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTG                                                       <  SRR4302540.180997/94‑1 (MQ=60)

                                        TAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTG                                                       >  SRR4302540.2759822/1‑94 (MQ=60)

                                         AGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGA                                                      <  SRR4302540.1452394/94‑1 (MQ=60)

                                         AGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGA                                                      <  SRR4302540.7036586/94‑1 (MQ=60)

                                          GCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGAT                                                     <  SRR4302540.5309072/94‑1 (MQ=60)

                                           CCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATA                                                    >  SRR4302540.6071116/1‑94 (MQ=60)

                                           CCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATA                                                    >  SRR4302540.6071115/1‑94 (MQ=60)

                                            CGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATAC                                                   <  SRR4302540.8325352/94‑1 (MQ=60)

                                            CGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATAC                                                   <  SRR4302540.5481576/94‑1 (MQ=60)

                                            CGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATAC                                                   >  SRR4302540.6287253/1‑94 (MQ=60)

                                             GGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACC                                                  >  SRR4302540.5135613/1‑94 (MQ=60)

                                             GGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACC                                                  >  SRR4302540.3137321/1‑94 (MQ=60)

                                              GAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCA                                                 >  SRR4302540.6380268/1‑94 (MQ=60)

                                              GAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCA                                                 <  SRR4302540.883853/94‑1 (MQ=60)

                                              GAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCA                                                 >  SRR4302540.6380267/1‑94 (MQ=60)

                                               AAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAG                                                <  SRR4302540.1723038/94‑1 (MQ=60)

                                                AGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGC                                               >  SRR4302540.4520433/1‑94 (MQ=60)

                                                AGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGC                                               >  SRR4302540.9416057/1‑94 (MQ=60)

                                                AGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGATCATATTATTTGGTTGATACCAGC                                               >  SRR4302540.9416250/1‑94 (MQ=60)

                                                 GCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTTCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCT                                              >  SRR4302540.5729243/1‑94 (MQ=60)

                                                 GCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCG                                              >  SRR4302540.9955784/1‑94 (MQ=60)

                                                 GCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGACATATTATTTGGTTGATACCAGCG                                              >  SRR4302540.5729505/1‑94 (MQ=60)

                                                 ACTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCG                                              <  SRR4302540.5225230/94‑1 (MQ=60)

                                                   TTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTT                                            >  SRR4302540.2285237/1‑94 (MQ=60)

                                                   TTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTT                                            >  SRR4302540.4307446/1‑94 (MQ=60)

                                                   TTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGCCATATTATTTGGTTGATACCAGCGTT                                            >  SRR4302540.9748532/1‑94 (MQ=60)

                                                    TACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTG                                           <  SRR4302540.4595212/94‑1 (MQ=60)

                                                     ACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGA                                          <  SRR4302540.3183915/94‑1 (MQ=60)

                                                     ACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGA                                          >  SRR4302540.7495240/1‑94 (MQ=60)

                                                     ACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGA                                          >  SRR4302540.7495239/1‑94 (MQ=60)

                                                      CTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGAT                                         <  SRR4302540.9186300/94‑1 (MQ=60)

                                                      CTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGAT                                         >  SRR4302540.4305748/1‑94 (MQ=60)

                                                       TTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATA                                        <  SRR4302540.4903125/94‑1 (MQ=60)

                                                         AAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAA                                      <  SRR4302540.103849/94‑1 (MQ=60)

                                                         AAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAA                                      >  SRR4302540.6474786/1‑94 (MQ=60)

                                                           ATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAAT                                    >  SRR4302540.6132890/1‑94 (MQ=60)

                                                           ATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAAT                                    >  SRR4302540.8561194/1‑94 (MQ=60)

                                                           ATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAAT                                    >  SRR4302540.6132889/1‑94 (MQ=60)

                                                            TTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATT                                   >  SRR4302540.9727029/1‑94 (MQ=60)

                                                            TTATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATT                                   <  SRR4302540.1942353/94‑1 (MQ=60)

                                                             TATCCTTTATCAGCGCAAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTG                                  <  SRR4302540.2707608/94‑1 (MQ=60)

                                                              ATCCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGG                                 >  SRR4302540.8889551/1‑94 (MQ=60)

                                                              ATCCTTTATCAGCGCCAGCGCTTCTTTACAGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGT                                 >  SRR4302540.1129562/1‑94 (MQ=60)

                                                                CCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCG                               >  SRR4302540.2245512/1‑94 (MQ=60)

                                                                CCTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCG                               >  SRR4302540.5179823/1‑94 (MQ=60)

                                                                 CTTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGC                              >  SRR4302540.7314328/1‑94 (MQ=60)

                                                                  TTTATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCA                             >  SRR4302540.8874734/1‑94 (MQ=60)

                                                                     ATCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATT                          >  SRR4302540.7575247/1‑94 (MQ=60)

                                                                      TCAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTG                         <  SRR4302540.1213518/94‑1 (MQ=60)

                                                                      ACAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTG                         <  SRR4302540.8692592/94‑1 (MQ=60)

                                                                      ACAGCCCCAGCGCCTCTTTACCGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTG                         <  SRR4302540.8692594/94‑1 (MQ=60)

                                                                       CAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGT                        <  SRR4302540.1679622/94‑1 (MQ=60)

                                                                       CAGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGT                        <  SRR4302540.7542946/94‑1 (MQ=60)

                                                                        AGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTG                       <  SRR4302540.5174151/94‑1 (MQ=60)

                                                                        AGCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTG                       >  SRR4302540.4679424/1‑94 (MQ=60)

                                                                         GCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGC                      <  SRR4302540.2619317/94‑1 (MQ=60)

                                                                         GCGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGC                      <  SRR4302540.2619320/94‑1 (MQ=60)

                                                                          CGCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCC                     >  SRR4302540.783383/1‑94 (MQ=60)

                                                                           GCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCG                    >  SRR4302540.2271706/1‑94 (MQ=60)

                                                                           GCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCG                    <  SRR4302540.9231826/94‑1 (MQ=60)

                                                                           GCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCG                    >  SRR4302540.8338367/1‑94 (MQ=60)

                                                                           GCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCG                    <  SRR4302540.4633000/94‑1 (MQ=60)

                                                                           GCCAGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCG                    >  SRR4302540.6892363/1‑94 (MQ=60)

                                                                            CCAGCCCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGG                   <  SRR4302540.7959389/94‑1 (MQ=60)

                                                                              AGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTG                 >  SRR4302540.9833427/1‑94 (MQ=60)

                                                                              AGCGCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTG                 <  SRR4302540.5252523/94‑1 (MQ=60)

                                                                               GCGCTTCTTTACTGCCGATATCGCCGACAACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGT                <  SRR4302540.1036306/94‑1 (MQ=60)

                                                                                 GCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGC              >  SRR4302540.5166273/1‑94 (MQ=60)

                                                                                 GCTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGA              >  SRR4302540.8583228/1‑94 (MQ=60)

                                                                                  CTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGAC             >  SRR4302540.3533002/1‑94 (MQ=60)

                                                                                  CTTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGAC             >  SRR4302540.8504391/1‑94 (MQ=60)

                                                                                  CTTCTTTACTGCCGATATCGCCGACCACGATAAAGGCCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGGGAC             >  SRR4302540.2503774/1‑94 (MQ=60)

                                                                                   TTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACC            <  SRR4302540.8654050/94‑1 (MQ=60)

                                                                                   TTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACC            <  SRR4302540.8878531/94‑1 (MQ=60)

                                                                                   TTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACC            <  SRR4302540.8654049/94‑1 (MQ=60)

                                                                                   TTCTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACC            >  SRR4302540.123539/1‑94 (MQ=60)

                                                                                     CTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGT          <  SRR4302540.504009/94‑1 (MQ=60)

                                                                                     CTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGT          >  SRR4302540.252452/1‑94 (MQ=60)

                                                                                     CTTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGG          >  SRR4302540.5639130/1‑94 (MQ=60)

                                                                                      TTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTG         <  SRR4302540.858151/94‑1 (MQ=60)

                                                                                      TTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTG         <  SRR4302540.25059/94‑1 (MQ=60)

                                                                                      TTTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTG         <  SRR4302540.3123072/94‑1 (MQ=60)

                                                                                       TTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGG        <  SRR4302540.8111451/94‑1 (MQ=60)

                                                                                       TTACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGG        <  SRR4302540.8642220/94‑1 (MQ=60)

                                                                                         ACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCG      <  SRR4302540.9389916/94‑1 (MQ=60)

                                                                                         ACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCG      >  SRR4302540.284713/1‑94 (MQ=60)

                                                                                         ACTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCG      >  SRR4302540.5348756/1‑94 (MQ=60)

                                                                                          CTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCGA     >  SRR4302540.9566814/1‑94 (MQ=60)

                                                                                          CTGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCGA     >  SRR4302540.3744395/1‑94 (MQ=60)

                                                                                           TGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCGAC    >  SRR4302540.4764608/1‑94 (MQ=60)

                                                                                           TGCCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCGAC    >  SRR4302540.8715543/1‑94 (MQ=60)

                                                                                             CCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCGACAG  <  SRR4302540.4847166/94‑1 (MQ=60)

TCGGCTTTTGTCGGCCAGACGCGATTTTCAGCTGCTTTGTTAGCCGGAAGCTTACTTAAATTATCCTTTATCAGCGCCAGCGCTTCTTTACTGTCGATATCGCCGACCACGATAAAGGTCATATTATTTGGTTGATACCAGCGTTGATAAAATTGGCGCAATTGTGCCGGTGTGACCGTGGCGACAG  >  NC_000913/1572433‑1572619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`