Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,664,091	T→C	S271S (AGT→AGC)	b1592 →	putative chloride channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,664,091	0	T	C	100.0%	654.3 / NA	201	S271S (AGT→AGC)	b1592	putative chloride channel
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (93/108); total (93/108)

Only 100 of 214 total aligned reads displayed.

TGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGG  >  NC_000913/1664001‑1664180

tGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGcct                                                                                         >  1:4141072/1‑93 (MQ=255)

  cgcTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCa                                                                                       <  2:9556451/93‑1 (MQ=255)

   gcTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCaa                                                                                      <  1:3640724/93‑1 (MQ=255)

   gcTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCaa                                                                                      >  2:4173700/1‑93 (MQ=255)

    cTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCaaa                                                                                     <  1:2182194/93‑1 (MQ=255)

     tGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAAc                                                                                    <  1:8618987/93‑1 (MQ=255)

      gATTATCAGTACAGGTGTACTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACt                                                                                   >  2:9835756/1‑93 (MQ=255)

        ttATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTg                                                                                 <  1:9029922/93‑1 (MQ=255)

         tATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTgc                                                                                >  2:1530443/1‑93 (MQ=255)

           tCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTgcgc                                                                              <  2:970052/93‑1 (MQ=255)

             aGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGcgccg                                                                            <  1:9330230/93‑1 (MQ=255)

                 cAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCt                                                                        >  1:8343425/1‑93 (MQ=255)

                  aGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTg                                                                       <  2:2115920/93‑1 (MQ=255)

                   ggTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTgg                                                                      >  1:336283/1‑93 (MQ=255)

                   ggTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTgg                                                                      >  1:2046026/1‑93 (MQ=255)

                    gtgtGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTTAAACTTTCGCCGCCCTGGc                                                                     >  2:8972522/1‑93 (MQ=255)

                      gtgCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCaa                                                                   <  2:5722822/93‑1 (MQ=255)

                       tgCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAAc                                                                  <  2:104544/93‑1 (MQ=255)

                       tgCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAAc                                                                  <  2:6999894/93‑1 (MQ=255)

                        gCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACt                                                                 <  2:3345626/93‑1 (MQ=255)

                         cTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTg                                                                <  1:1570439/93‑1 (MQ=255)

                          tGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTgg                                                               >  2:452558/1‑93 (MQ=255)

                          tGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTgg                                                               <  2:9090793/93‑1 (MQ=255)

                          tGGAAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCCCCGCCCTGGCAACTgg                                                               >  2:518570/1‑93 (MQ=255)

                           ggCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGc                                                              >  1:5164870/1‑93 (MQ=255)

                           ggCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGc                                                              >  1:6915541/1‑93 (MQ=255)

                               ggTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTa                                                          <  1:72150/93‑1 (MQ=255)

                               ggTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTa                                                          <  1:5382186/93‑1 (MQ=255)

                                gTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAg                                                         <  1:1205516/93‑1 (MQ=255)

                                gTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAg                                                         >  2:9220603/1‑93 (MQ=255)

                                 tCTGTGCGGACCACTGTTGTTAACGTTAATGAACTCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAgg                                                        <  2:6529856/93‑1 (MQ=255)

                                 tCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAgg                                                        <  1:6237791/93‑1 (MQ=255)

                                   tgtgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCg                                                      <  2:7530071/93‑1 (MQ=255)

                                   tgtgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCg                                                      >  1:534012/1‑93 (MQ=255)

                                   tgtgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCg                                                      >  2:496666/1‑93 (MQ=255)

                                   tgtgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCg                                                      >  1:7735675/1‑93 (MQ=255)

                                   tgtgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCg                                                      <  2:3581919/93‑1 (MQ=255)

                                   tgtgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCg                                                      >  2:2720481/1‑93 (MQ=255)

                                    gtgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCgg                                                     >  1:5972161/1‑93 (MQ=255)

                                     tgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCggg                                                    >  1:3050620/1‑93 (MQ=255)

                                     tgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCggg                                                    <  1:319801/93‑1 (MQ=255)

                                     tgCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCggg                                                    >  1:9660367/1‑93 (MQ=255)

                                      gCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGt                                                   >  2:4472501/1‑93 (MQ=255)

                                         gACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGa                                                >  1:4736001/1‑93 (MQ=255)

                                         gACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGa                                                <  2:3978617/93‑1 (MQ=255)

                                          gccACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGAt                                               <  1:3554667/92‑1 (MQ=255)

                                          aCCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGAt                                               >  1:5482694/1‑93 (MQ=255)

                                           cccgtgttgtTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATc                                              <  2:7428750/89‑1 (MQ=255)

                                            cACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCg                                             <  1:408634/93‑1 (MQ=255)

                                            cACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCg                                             >  1:3504350/1‑93 (MQ=255)

                                            cACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCg                                             <  1:8422273/93‑1 (MQ=255)

                                             aCTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCgg                                            >  1:9103000/1‑92 (MQ=255)

                                             aCTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGt                                            <  2:8831786/93‑1 (MQ=255)

                                               tgttgtTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTgg                                          >  2:4296470/1‑93 (MQ=255)

                                                gttgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTggg                                         >  1:810792/1‑93 (MQ=255)

                                                 ttgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGt                                        <  1:3820491/93‑1 (MQ=255)

                                                 ttgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGt                                        <  2:966213/93‑1 (MQ=255)

                                                  tgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTc                                       <  1:2270351/93‑1 (MQ=255)

                                                  tgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTc                                       >  2:5447287/1‑93 (MQ=255)

                                                     tAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTgt                                    >  2:232005/1‑92 (MQ=255)

                                                      aaCGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCt                                   >  2:407206/1‑93 (MQ=255)

                                                        cGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCttt                                 <  1:8587827/93‑1 (MQ=255)

                                                         gTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTc                                >  2:3840571/1‑93 (MQ=255)

                                                         gTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTc                                <  2:1882823/93‑1 (MQ=255)

                                                          ttAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTATGCGGGTTGATCGTGGGTCTGCTTTcc                               >  2:6211624/1‑93 (MQ=255)

                                                          ttAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCCTTcc                               >  1:2012195/1‑93 (MQ=255)

                                                           tAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTccc                              <  2:9388628/93‑1 (MQ=255)

                                                           tAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTccc                              <  2:3190291/93‑1 (MQ=255)

                                                            aaTGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCt                             <  1:8203645/93‑1 (MQ=255)

                                                            aaTGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCt                             >  1:5216267/1‑93 (MQ=255)

                                                            aaTGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCt                             <  2:5550627/93‑1 (MQ=255)

                                                            aaTGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCt                             <  2:6855785/93‑1 (MQ=255)

                                                             aTGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTg                            >  2:4332446/1‑93 (MQ=255)

                                                              tGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGt                           >  1:8994195/1‑93 (MQ=255)

                                                              tGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGt                           <  2:795186/93‑1 (MQ=255)

                                                               gAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGGTGATCGTGGGTCTGCTTTCCCTGtt                          >  2:8824960/1‑93 (MQ=255)

                                                                aaCGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTc                         >  2:8742684/1‑93 (MQ=255)

                                                                 aCGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTca                        <  2:8493153/93‑1 (MQ=255)

                                                                    ccTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCacac                     >  2:81876/1‑93 (MQ=255)

                                                                    ccTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCacac                     >  2:8408731/1‑93 (MQ=255)

                                                                    ccTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCacac                     >  2:7621354/1‑93 (MQ=255)

                                                                     cTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACAcc                    <  1:3609740/93‑1 (MQ=255)

                                                                      tGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCt                   >  1:463138/1‑93 (MQ=255)

                                                                         cATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCa                >  1:9888059/1‑93 (MQ=255)

                                                                         cATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCa                >  1:7384378/1‑93 (MQ=255)

                                                                          aTCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAg               <  2:2003003/93‑1 (MQ=255)

                                                                          aTCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAg               <  1:7093733/93‑1 (MQ=255)

                                                                           tCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAgt              >  1:2723988/1‑93 (MQ=255)

                                                                             gTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAgtgt            >  1:9439778/1‑93 (MQ=255)

                                                                              tGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGtgtg           >  2:7884032/1‑93 (MQ=255)

                                                                              tGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGtgtg           >  1:5789087/1‑93 (MQ=255)

                                                                               ggATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTgg          <  2:582067/93‑1 (MQ=255)

                                                                               ggATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTgg          <  1:212544/93‑1 (MQ=255)

                                                                                gATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTggg         <  1:6008726/93‑1 (MQ=255)

                                                                                gATTTATGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTggg         <  1:843264/93‑1 (MQ=255)

                                                                                    tgtgAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCaa     <  2:4557158/93‑1 (MQ=255)

                                                                                     gtgAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAAc    >  1:4373961/1‑93 (MQ=255)

                                                                                     gtgAGCCGCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAAc    <  1:1854692/93‑1 (MQ=255)

                                                                                      tgAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACg   <  1:6682991/93‑1 (MQ=255)

                                                                                       gAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACgg  <  1:3178776/93‑1 (MQ=255)

TGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGG  >  NC_000913/1664001‑1664180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTAT  >  NC_000913/1663998‑1664184

CTATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGGTAATGAACGCCTGTCATCGTGGATTTGTGAGC                                                                                               >  SRR4302540.8508141/1‑94 (MQ=60)

 TATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCC                                                                                              <  SRR4302540.9719932/94‑1 (MQ=60)

 TATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCC                                                                                              >  SRR4302540.8837916/1‑94 (MQ=60)

  ATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCT                                                                                             >  SRR4302540.3660462/1‑94 (MQ=60)

  ATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCT                                                                                             >  SRR4302540.4185876/1‑94 (MQ=60)

  ATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCT                                                                                             >  SRR4302540.4185878/1‑94 (MQ=60)

  ATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCT                                                                                             <  SRR4302540.4405789/94‑1 (MQ=60)

   TGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTC                                                                                            <  SRR4302540.3459867/94‑1 (MQ=60)

      GCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAA                                                                                         <  SRR4302540.3680594/94‑1 (MQ=60)

       CTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAAC                                                                                        <  SRR4302540.2205741/94‑1 (MQ=60)

        TGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACT                                                                                       >  SRR4302540.4187891/1‑94 (MQ=60)

        TGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACT                                                                                       <  SRR4302540.2172851/94‑1 (MQ=60)

        TGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACT                                                                                       <  SRR4302540.8719106/94‑1 (MQ=60)

         GATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTT                                                                                      <  SRR4302540.539299/94‑1 (MQ=60)

           TTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGC                                                                                    <  SRR4302540.9138284/94‑1 (MQ=60)

            TATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCG                                                                                   >  SRR4302540.8710606/1‑94 (MQ=60)

             ATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGC                                                                                  >  SRR4302540.6163577/1‑94 (MQ=60)

               CAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCG                                                                                <  SRR4302540.4489105/94‑1 (MQ=60)

               CAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCAG                                                                                >  SRR4302540.5184609/1‑94 (MQ=60)

                AGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGC                                                                               >  SRR4302540.9881018/1‑94 (MQ=60)

                AGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGC                                                                               <  SRR4302540.5886050/94‑1 (MQ=60)

                AGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGC                                                                               >  SRR4302540.2350683/1‑94 (MQ=60)

                AGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGC                                                                               <  SRR4302540.9441483/94‑1 (MQ=60)

                   ACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCT                                                                            >  SRR4302540.8440267/1‑94 (MQ=60)

                    CAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTG                                                                           <  SRR4302540.6068576/94‑1 (MQ=60)

                     AGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGG                                                                          >  SRR4302540.2068069/1‑94 (MQ=60)

                     AGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGG                                                                          >  SRR4302540.339670/1‑94 (MQ=60)

                       GTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCA                                                                        >  SRR4302540.5431674/1‑94 (MQ=60)

                         GTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAAC                                                                      <  SRR4302540.3696090/94‑1 (MQ=60)

                         GTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAAC                                                                      >  SRR4302540.1892025/1‑94 (MQ=60)

                         GTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAAC                                                                      <  SRR4302540.3395506/94‑1 (MQ=60)

                         GTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCCGGCAAC                                                                      >  SRR4302540.6985347/1‑94 (MQ=60)

                            CTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGG                                                                   <  SRR4302540.1587650/94‑1 (MQ=60)

                             TGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGC                                                                  >  SRR4302540.6992493/1‑94 (MQ=60)

                             TGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGC                                                                  >  SRR4302540.5221033/1‑94 (MQ=60)

                                CAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACT                                                               <  SRR4302540.8654134/94‑1 (MQ=60)

                                 AGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTA                                                              >  SRR4302540.5474699/1‑94 (MQ=60)

                                  GGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAG                                                             <  SRR4302540.72890/94‑1 (MQ=60)

                                  GGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAG                                                             <  SRR4302540.5441384/94‑1 (MQ=60)

                                   GTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGG                                                            <  SRR4302540.1217890/94‑1 (MQ=60)

                                   GTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGG                                                            >  SRR4302540.6374700/1‑94 (MQ=60)

                                   GTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGG                                                            <  SRR4302540.3558584/94‑1 (MQ=60)

                                    TCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGC                                                           <  SRR4302540.9628961/94‑1 (MQ=60)

                                    TCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGC                                                           <  SRR4302540.6307191/94‑1 (MQ=60)

                                     CTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCG                                                          >  SRR4302540.7825988/1‑94 (MQ=60)

                                     CTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCG                                                          >  SRR4302540.539787/1‑94 (MQ=60)

                                      TGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGG                                                         >  SRR4302540.6038261/1‑94 (MQ=60)

                                       GTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGG                                                        >  SRR4302540.9774080/1‑94 (MQ=60)

                                       GTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGG                                                        >  SRR4302540.3605092/1‑94 (MQ=60)

                                       GTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGG                                                        >  SRR4302540.3084092/1‑94 (MQ=60)

                                        TGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGT                                                       <  SRR4302540.322998/94‑1 (MQ=60)

                                        TGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGT                                                       <  SRR4302540.322997/94‑1 (MQ=60)

                                          CGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTG                                                     >  SRR4302540.8967320/1‑94 (MQ=60)

                                           GGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGA                                                    >  SRR4302540.4786558/1‑94 (MQ=60)

                                           GGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGA                                                    >  SRR4302540.4786559/1‑94 (MQ=60)

                                            GACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGGGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGGTGAT                                                   >  SRR4302540.7166346/1‑94 (MQ=60)

                                            GACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGAT                                                   <  SRR4302540.5840684/94‑1 (MQ=60)

                                            GACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGAT                                                   >  SRR4302540.5543052/1‑94 (MQ=60)

                                             GCCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATC                                                  <  SRR4302540.3593505/94‑1 (MQ=60)

                                             ACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATC                                                  <  SRR4302540.3593506/94‑1 (MQ=60)

                                              CCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCG                                                 >  SRR4302540.3542526/1‑94 (MQ=60)

                                               CACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGT                                                <  SRR4302540.3965920/94‑1 (MQ=60)

                                               CACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGT                                                <  SRR4302540.7302982/94‑1 (MQ=60)

                                               CACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGT                                                >  SRR4302540.9212384/1‑94 (MQ=60)

                                               CACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGT                                                >  SRR4302540.2122202/1‑94 (MQ=60)

                                               CACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGT                                                <  SRR4302540.8520032/94‑1 (MQ=60)

                                               CACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGT                                                <  SRR4302540.412912/94‑1 (MQ=60)

                                               CACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGG                                                >  SRR4302540.9212385/1‑94 (MQ=60)

                                                ACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTG                                               <  SRR4302540.9260909/94‑1 (MQ=60)

                                                 CTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGG                                              >  SRR4302540.5249199/1‑94 (MQ=60)

                                                  TGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGG                                             >  SRR4302540.819403/1‑94 (MQ=60)

                                                    TTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTC                                           <  SRR4302540.3862074/94‑1 (MQ=60)

                                                     TGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCT                                          <  SRR4302540.2294711/94‑1 (MQ=60)

                                                      GTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTG                                         >  SRR4302540.8469825/1‑94 (MQ=60)

                                                         AACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTT                                      <  SRR4302540.9726236/94‑1 (MQ=60)

                                                          ACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTT                                     <  SRR4302540.7391411/94‑1 (MQ=60)

                                                           CGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTC                                    <  SRR4302540.8687658/94‑1 (MQ=60)

                                                           CGTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTC                                    <  SRR4302540.8687657/94‑1 (MQ=60)

                                                            GTTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCCTTCC                                   >  SRR4302540.2033955/1‑94 (MQ=60)

                                                             TTAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCC                                  <  SRR4302540.183190/94‑1 (MQ=60)

                                                              TAATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCT                                 >  SRR4302540.5273123/1‑94 (MQ=60)

                                                               AATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTG                                <  SRR4302540.8298980/94‑1 (MQ=60)

                                                                ATGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGT                               >  SRR4302540.9102084/1‑94 (MQ=60)

                                                                 TGAACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTT                              >  SRR4302540.1279004/1‑94 (MQ=60)

                                                                   AACGCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCA                            >  SRR4302540.9133656/1‑94 (MQ=60)

                                                                      GCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACAC                         >  SRR4302540.2256737/1‑94 (MQ=60)

                                                                      GCCTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACAC                         <  SRR4302540.7765590/94‑1 (MQ=60)

                                                                        CTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCT                       <  SRR4302540.2302583/94‑1 (MQ=60)

                                                                        CTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCT                       >  SRR4302540.5893749/1‑94 (MQ=60)

                                                                        CTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCT                       <  SRR4302540.3649193/94‑1 (MQ=60)

                                                                        CTGTCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCT                       >  SRR4302540.468079/1‑94 (MQ=60)

                                                                           TCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCA                    >  SRR4302540.10003515/1‑94 (MQ=60)

                                                                           TCATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCA                    >  SRR4302540.7466654/1‑94 (MQ=60)

                                                                             ATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGT                  <  SRR4302540.7172674/94‑1 (MQ=60)

                                                                             ATCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGT                  >  SRR4302540.2753359/1‑94 (MQ=60)

                                                                              TCGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTG                 <  SRR4302540.2994119/94‑1 (MQ=60)

                                                                               CGTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGT                >  SRR4302540.9551879/1‑94 (MQ=60)

                                                                                GTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTG               <  SRR4302540.2052886/94‑1 (MQ=60)

                                                                                GTGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTG               >  SRR4302540.5853054/1‑94 (MQ=60)

                                                                                 TGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGG              <  SRR4302540.10016176/94‑1 (MQ=60)

                                                                                 TGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGG              <  SRR4302540.4688226/94‑1 (MQ=60)

                                                                                 TGGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGG              <  SRR4302540.9508809/94‑1 (MQ=60)

                                                                                  GGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGG             <  SRR4302540.214625/94‑1 (MQ=60)

                                                                                  GGATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGG             <  SRR4302540.827173/94‑1 (MQ=60)

                                                                                   GATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGG            <  SRR4302540.852206/94‑1 (MQ=60)

                                                                                   GATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGG            <  SRR4302540.6075352/94‑1 (MQ=60)

                                                                                   GATTTATGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGG            <  SRR4302540.852207/94‑1 (MQ=60)

                                                                                    ATTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGC           >  SRR4302540.7255666/1‑94 (MQ=60)

                                                                                     TTTGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCA          <  SRR4302540.8436589/94‑1 (MQ=60)

                                                                                       TGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAAC        >  SRR4302540.3198706/1‑94 (MQ=60)

                                                                                       TGTGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAAC        >  SRR4302540.4421463/1‑94 (MQ=60)

                                                                                        GTGAGCCGCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACG       <  SRR4302540.1875032/94‑1 (MQ=60)

                                                                                         TGAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGG      <  SRR4302540.6756804/94‑1 (MQ=60)

                                                                                          GAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGC     <  SRR4302540.3213317/94‑1 (MQ=60)

                                                                                          GAGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGC     >  SRR4302540.2265994/1‑94 (MQ=60)

                                                                                           AGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCT    <  SRR4302540.6838578/94‑1 (MQ=60)

                                                                                           AGCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCT    <  SRR4302540.6838579/94‑1 (MQ=60)

                                                                                            GCCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTA   >  SRR4302540.7862987/1‑94 (MQ=60)

                                                                                             CCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTAT  <  SRR4302540.7164836/94‑1 (MQ=60)

CTATGCGCTGATTATCAGTACAGGTGTGCTGGCAGGTCTGTGCGGACCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTAT  >  NC_000913/1663998‑1664184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`