Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,672,562 T→C L209P (CTG→CCG)  b1601 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,672,5620TC99.5% 619.9 / ‑5.4 197L209P (CTG→CCG) b1601putative transport protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (89/107);  total (89/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 209 total aligned reads displayed.

CGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATA  >  NC_000913/1672472‑1672651
                                                                                          |                                                                                         
 ggTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGt                                                                                        <  2:963371/93‑1 (MQ=255)
 ggTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGt                                                                                        <  2:6334784/93‑1 (MQ=255)
  gTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTc                                                                                       >  1:439588/1‑93 (MQ=255)
   ttGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCg                                                                                      >  2:3302503/1‑93 (MQ=255)
   ttGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCg                                                                                      >  1:5772139/1‑93 (MQ=255)
      aaGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCt                                                                                   <  2:3127595/93‑1 (MQ=255)
      aaGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCt                                                                                   <  1:3514322/93‑1 (MQ=255)
      aaGGGAGGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCt                                                                                   <  2:1409358/93‑1 (MQ=255)
       aGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTg                                                                                  <  2:984246/93‑1 (MQ=255)
       aGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTg                                                                                  <  2:3897483/93‑1 (MQ=255)
       aGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTg                                                                                  <  2:3833373/93‑1 (MQ=255)
       aGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTg                                                                                  >  1:5689777/1‑93 (MQ=255)
        gggATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTgg                                                                                 >  2:4600462/1‑93 (MQ=255)
           aTGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCg                                                                              >  2:2163410/1‑93 (MQ=255)
            tGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGa                                                                             <  2:6307915/93‑1 (MQ=255)
            tGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGa                                                                             <  1:4369673/93‑1 (MQ=255)
            tGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGa                                                                             <  2:6985767/93‑1 (MQ=255)
            tGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGa                                                                             >  2:8626005/1‑93 (MQ=255)
            tGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGAGCGa                                                                             >  2:9239573/1‑93 (MQ=255)
             ggcggcGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGAt                                                                            <  2:4066100/93‑1 (MQ=255)
             ggcggcGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGAt                                                                            >  1:572377/1‑93 (MQ=255)
              gcggggATTCCAAGTGCGATTGGCCGTTTTTCCTCTTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATg                                                                           <  2:8712215/93‑1 (MQ=255)
              gcggcgATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATg                                                                           >  1:4768705/1‑93 (MQ=255)
                  cgATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTcg                                                                       <  2:2389200/93‑1 (MQ=255)
                    aTTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTcgcc                                                                     >  1:9115288/1‑93 (MQ=255)
                     ttCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTcgccc                                                                    >  2:7976307/1‑92 (MQ=255)
                       cAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCa                                                                  >  2:10015327/1‑93 (MQ=255)
                        aaCGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCAc                                                                 >  1:8530914/1‑93 (MQ=255)
                         aCGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACt                                                                <  2:5675004/93‑1 (MQ=255)
                           gTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCg                                                              >  2:4199960/1‑93 (MQ=255)
                                aTTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGtt                                                         >  1:263039/1‑93 (MQ=255)
                                 ttGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttc                                                        >  1:4555209/1‑93 (MQ=255)
                                 ttGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttc                                                        >  2:6900699/1‑93 (MQ=255)
                                  tGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcg                                                       <  2:5469906/93‑1 (MQ=255)
                                   gACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgc                                                      <  1:4983962/93‑1 (MQ=255)
                                   gACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgc                                                      <  2:7592776/93‑1 (MQ=255)
                                    aCAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgct                                                     >  1:7231425/1‑93 (MQ=255)
                                    aCAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgct                                                     >  2:251774/1‑93 (MQ=255)
                                    aCAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgct                                                     <  2:7854225/93‑1 (MQ=255)
                                     cAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgctt                                                    <  2:6476235/93‑1 (MQ=255)
                                      aGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgcttc                                                   >  1:7023544/1‑93 (MQ=255)
                                       gtgtTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgcttcg                                                  >  2:3690207/1‑93 (MQ=255)
                                        tgtTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgcttcgc                                                 <  1:5336027/93‑1 (MQ=255)
                                          tttCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTcgcttcgctt                                               >  1:1796310/1‑93 (MQ=255)
                                            tctcATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCtttt                                             <  1:2977899/93‑1 (MQ=255)
                                            tctcATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCtttt                                             <  2:1179326/93‑1 (MQ=255)
                                            tctcATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCtttt                                             <  2:1522248/93‑1 (MQ=255)
                                            tctcATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCtttt                                             >  2:9744007/1‑93 (MQ=255)
                                             ctcATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTg                                            <  1:1651067/93‑1 (MQ=255)
                                              tcATTATCTTGGGCTGAAAACAACCATCAGAATCATCACCCGCCCGGTCGGCTTGGGGGTGCTCGCCGCACTCGATGGTTGCTTCGCTTTTGt                                           >  2:7090331/1‑93 (MQ=255)
                                                aTTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCt                                         <  1:1525767/93‑1 (MQ=255)
                                                aTTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCt                                         <  2:1510007/93‑1 (MQ=255)
                                                 ttATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTg                                        <  1:4089962/93‑1 (MQ=255)
                                                  tATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTgg                                       <  1:3809750/93‑1 (MQ=255)
                                                  tATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTgg                                       <  1:686845/93‑1 (MQ=255)
                                                    tCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTgggg                                     >  2:7201186/1‑93 (MQ=255)
                                                    tCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTgggg                                     >  1:8570918/1‑93 (MQ=255)
                                                     cTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGa                                    <  2:2617949/93‑1 (MQ=255)
                                                     cTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGa                                    <  1:3950188/93‑1 (MQ=255)
                                                     cTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGa                                    <  1:7516116/93‑1 (MQ=255)
                                                      tGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAt                                   <  2:2746617/93‑1 (MQ=255)
                                                      tGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAt                                   >  2:6881857/1‑93 (MQ=255)
                                                         tGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGc                                <  1:4753407/93‑1 (MQ=255)
                                                         tGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGc                                >  1:5253833/1‑93 (MQ=255)
                                                         tGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGc                                >  1:1616296/1‑93 (MQ=255)
                                                          gCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCt                               >  1:8692964/1‑93 (MQ=255)
                                                           cTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTg                              <  2:19446/93‑1 (MQ=255)
                                                            tGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTgg                             <  1:6067658/93‑1 (MQ=255)
                                                             gAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGc                            >  1:3998677/1‑93 (MQ=255)
                                                             gAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGc                            >  2:1918360/1‑93 (MQ=255)
                                                               aaaCAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCt                          <  1:6436697/93‑1 (MQ=255)
                                                                aaCAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCtt                         <  2:5889429/93‑1 (MQ=255)
                                                                aaCAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCtt                         >  1:7591796/1‑93 (MQ=255)
                                                                 aCAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCttt                        >  1:2430560/1‑93 (MQ=255)
                                                                 aCAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCttt                        <  2:1600432/93‑1 (MQ=255)
                                                                 aCAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCttt                        <  2:9076248/93‑1 (MQ=255)
                                                                 aCAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCttt                        <  2:6090349/93‑1 (MQ=255)
                                                                  cAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTg                       <  1:9335871/93‑1 (MQ=255)
                                                                  cAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTg                       >  2:7426159/1‑93 (MQ=255)
                                                                  cAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTg                       <  1:1591179/93‑1 (MQ=255)
                                                                  cAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTg                       >  1:2395034/1‑93 (MQ=255)
                                                                      cATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCt                   >  2:2237992/1‑93 (MQ=255)
                                                                       aTCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCtt                  >  2:8923512/1‑93 (MQ=255)
                                                                        tCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTa                 >  1:3435520/1‑93 (MQ=255)
                                                                          cgCATCATCCGCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAAt               <  1:1932683/92‑1 (MQ=255)
                                                                          aGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAAt               <  2:1236047/93‑1 (MQ=255)
                                                                           gCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAAtt              >  1:4869922/1‑93 (MQ=255)
                                                                            catcatCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTa             >  2:3612508/1‑93 (MQ=255)
                                                                            catcatCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTa             >  1:9475726/1‑93 (MQ=255)
                                                                             atcatcACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTAc            >  1:7624668/1‑93 (MQ=255)
                                                                              tcatcaCCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACa           <  2:3174735/93‑1 (MQ=255)
                                                                                atcgCCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATc         <  1:8320111/93‑1 (MQ=255)
                                                                                 tcaCCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATcc        <  2:3992231/93‑1 (MQ=255)
                                                                                 acaCCGGCCCGGTCGCCTGAGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTAGGGATTGCTGGCCTTTGCGCTTAATTACATca        <  1:8969700/92‑2 (MQ=255)
                                                                                  caCCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATccc       <  2:8937788/93‑1 (MQ=255)
                                                                                  caCCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATccc       <  2:4495675/93‑1 (MQ=255)
                                                                                      ggCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAat   >  1:9136687/1‑93 (MQ=255)
                                                                                      ggCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAat   >  1:8158903/1‑93 (MQ=255)
                                                                                       gCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAata  <  1:1171225/93‑1 (MQ=255)
                                                                                       gCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAata  >  2:5034231/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATA  >  NC_000913/1672472‑1672651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGG  >  NC_000913/1672469‑1672655
                                                                                             |                                                                                             
GTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCC                                                                                               >  SRR4302540.7392655/1‑94 (MQ=60)
GTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCC                                                                                               >  SRR4302540.1481218/1‑94 (MQ=60)
 TCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCG                                                                                              <  SRR4302540.8346114/94‑1 (MQ=60)
    GGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTC                                                                                           >  SRR4302540.444264/1‑94 (MQ=60)
     GTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCG                                                                                          >  SRR4302540.5835901/1‑94 (MQ=60)
     GTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCG                                                                                          <  SRR4302540.7763223/94‑1 (MQ=60)
     GTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCG                                                                                          <  SRR4302540.9958410/94‑1 (MQ=60)
        GAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCT                                                                                       <  SRR4302540.1977166/94‑1 (MQ=60)
         AAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTG                                                                                      <  SRR4302540.3552608/94‑1 (MQ=60)
         AAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTG                                                                                      <  SRR4302540.6595836/94‑1 (MQ=60)
         AAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTG                                                                                      >  SRR4302540.5752413/1‑94 (MQ=60)
         AAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTG                                                                                      <  SRR4302540.7001684/94‑1 (MQ=60)
            GGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGC                                                                                   <  SRR4302540.4029417/94‑1 (MQ=60)
              ATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGA                                                                                 >  SRR4302540.6266227/1‑94 (MQ=60)
               TGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGAT                                                                                >  SRR4302540.578591/1‑94 (MQ=60)
               TGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGAT                                                                                <  SRR4302540.4417146/94‑1 (MQ=60)
                GGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATG                                                                               >  SRR4302540.4819634/1‑94 (MQ=60)
                      GATTCCACTTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAACCCGCCATCAGCATCCTCCCCGGCCCGGTCGCCTGGGCGATGCTCGCC                                                                         <  SRR4302540.3868770/94‑1 (MQ=60)
                      GATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCC                                                                         >  SRR4302540.9224852/1‑94 (MQ=60)
                       ATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCG                                                                        >  SRR4302540.7596187/1‑94 (MQ=60)
                          CAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCAC                                                                     <  SRR4302540.4112057/94‑1 (MQ=60)
                          CAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCAC                                                                     >  SRR4302540.447586/1‑94 (MQ=60)
                          CAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCAC                                                                     >  SRR4302540.8630037/1‑94 (MQ=60)
                           AACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACT                                                                    >  SRR4302540.8507779/1‑94 (MQ=60)
                              GTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGA                                                                 >  SRR4302540.5840961/1‑94 (MQ=60)
                               TGCGATTGACAGTTTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGAT                                                                <  SRR4302540.2432128/94‑1 (MQ=60)
                               TGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGAT                                                                <  SRR4302540.7225208/94‑1 (MQ=60)
                                  GATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTT                                                             >  SRR4302540.265604/1‑94 (MQ=60)
                                  GATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTT                                                             >  SRR4302540.1617645/1‑94 (MQ=60)
                                   ATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTC                                                            >  SRR4302540.4604459/1‑94 (MQ=60)
                                   ATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTC                                                            <  SRR4302540.4262437/94‑1 (MQ=60)
                                    TTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCG                                                           >  SRR4302540.9233888/1‑94 (MQ=60)
                                     TGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGC                                                          >  SRR4302540.3119005/1‑94 (MQ=60)
                                      GACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCT                                                         >  SRR4302540.7312066/1‑94 (MQ=60)
                                      GACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCT                                                         <  SRR4302540.5037920/94‑1 (MQ=60)
                                        CAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTC                                                       >  SRR4302540.7101558/1‑94 (MQ=60)
                                          GTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGC                                                     >  SRR4302540.9277217/1‑94 (MQ=60)
                                           TGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCT                                                    <  SRR4302540.5394514/94‑1 (MQ=60)
                                            GTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTT                                                   >  SRR4302540.1815854/1‑94 (MQ=60)
                                            GTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTT                                                   <  SRR4302540.8269275/94‑1 (MQ=60)
                                             TTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTT                                                  <  SRR4302540.8642366/94‑1 (MQ=60)
                                             TTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTT                                                  <  SRR4302540.8757858/94‑1 (MQ=60)
                                             TTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTT                                                  <  SRR4302540.3479264/94‑1 (MQ=60)
                                               TCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTG                                                <  SRR4302540.3010800/94‑1 (MQ=60)
                                                CTCATTATCTGGTGGTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGT                                               <  SRR4302540.1332596/94‑1 (MQ=60)
                                                CTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGT                                               <  SRR4302540.1669068/94‑1 (MQ=60)
                                                   ATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCGCTCGATGTTCGCTTCCCTTTTGTCTG                                            <  SRR4302540.8282540/94‑1 (MQ=60)
                                                   ATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTG                                            <  SRR4302540.1542665/94‑1 (MQ=60)
                                                   ATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTG                                            >  SRR4302540.5421774/1‑94 (MQ=60)
                                                    TTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGG                                           <  SRR4302540.4134077/94‑1 (MQ=60)
                                                    TTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGG                                           >  SRR4302540.4433642/1‑94 (MQ=60)
                                                     TATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGG                                          <  SRR4302540.3851242/94‑1 (MQ=60)
                                                     TATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGG                                          >  SRR4302540.3684461/1‑94 (MQ=60)
                                                     TATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGG                                          <  SRR4302540.694220/94‑1 (MQ=60)
                                                      ATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGG                                         >  SRR4302540.8670490/1‑94 (MQ=60)
                                                        CTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAT                                       <  SRR4302540.3992833/94‑1 (MQ=60)
                                                        CTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAT                                       >  SRR4302540.478058/1‑94 (MQ=60)
                                                        CTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAT                                       <  SRR4302540.4940912/94‑1 (MQ=60)
                                                        CTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAT                                       <  SRR4302540.7600154/94‑1 (MQ=60)
                                                        CTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAT                                       <  SRR4302540.7600151/94‑1 (MQ=60)
                                                         TGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATT                                      <  SRR4302540.7704321/94‑1 (MQ=60)
                                                          GGTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTG                                     <  SRR4302540.1605084/94‑1 (MQ=60)
                                                           GTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGC                                    >  SRR4302540.1634026/1‑94 (MQ=60)
                                                           GTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGC                                    <  SRR4302540.536540/94‑1 (MQ=60)
                                                           GTGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGC                                    >  SRR4302540.5311138/1‑94 (MQ=60)
                                                            TGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCT                                   >  SRR4302540.8793886/1‑94 (MQ=60)
                                                            TGCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCT                                   <  SRR4302540.4804129/94‑1 (MQ=60)
                                                             GCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTG                                  >  SRR4302540.5760172/1‑94 (MQ=60)
                                                             GCTGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTG                                  <  SRR4302540.5171335/94‑1 (MQ=60)
                                                               TGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGC                                <  SRR4302540.6135119/94‑1 (MQ=60)
                                                               TGAAAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGC                                >  SRR4302540.4041714/1‑94 (MQ=60)
                                                                  AAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTT                             <  SRR4302540.6508341/94‑1 (MQ=60)
                                                                  AAACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTT                             >  SRR4302540.7677067/1‑94 (MQ=60)
                                                                   AACAGCCATCAGCATCATCGCCGCCCTGGTCACATGGGCGATCCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTT                            <  SRR4302540.2755929/94‑1 (MQ=60)
                                                                   AACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTT                            >  SRR4302540.2456749/1‑94 (MQ=60)
                                                                   AACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTT                            <  SRR4302540.937367/94‑1 (MQ=60)
                                                                    ACAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTG                           >  SRR4302540.2420841/1‑94 (MQ=60)
                                                                    ACAGCCATCAGCATCATCACCGGCCCGGTCCCCTGGGCGATGCACGCCGCACTCGATGTTCGCTTCGCTTTTGTgtgcgtattgctgtgcgttg                           >  SRR4302540.2420840/1‑74 (MQ=60)
                                                                     CAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCCGGCCTTTGC                          <  SRR4302540.9447162/94‑1 (MQ=60)
                                                                     CAGCCATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGC                          <  SRR4302540.1608563/94‑1 (MQ=60)
                                                                         CATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTT                      <  SRR4302540.1831937/94‑1 (MQ=60)
                                                                          ATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTA                     >  SRR4302540.3495220/1‑94 (MQ=60)
                                                                          ATCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTA                     >  SRR4302540.3472868/1‑94 (MQ=60)
                                                                           TCAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAA                    <  SRR4302540.5791828/94‑1 (MQ=60)
                                                                            CAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAAT                   >  SRR4302540.4814521/1‑94 (MQ=60)
                                                                            CAGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAAT                   >  SRR4302540.261636/1‑94 (MQ=60)
                                                                             CGCATCATCCGCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATT                  <  SRR4302540.1953746/94‑1 (MQ=60)
                                                                             AGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATT                  >  SRR4302540.4922160/1‑94 (MQ=60)
                                                                             AGCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATT                  >  SRR4302540.4922161/1‑94 (MQ=60)
                                                                              GCATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTA                 >  SRR4302540.9588105/1‑94 (MQ=60)
                                                                               CATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTAC                >  SRR4302540.7710411/1‑94 (MQ=60)
                                                                               CATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTAC                >  SRR4302540.9520661/1‑94 (MQ=60)
                                                                                ATCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACA               >  SRR4302540.1676435/1‑94 (MQ=60)
                                                                                 TCATCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACAT              <  SRR4302540.8919624/94‑1 (MQ=60)
                                                                                   ATCGCCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCC            <  SRR4302540.8416688/94‑1 (MQ=60)
                                                                                    TCACCGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCC           <  SRR4302540.1665293/94‑1 (MQ=60)
                                                                                    ACACCGGCCCGGTCGCCTGAGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTAGGGATTGCTGGCCTTTGCGCTTAATTACATCAC           <  SRR4302540.9076151/94‑1 (MQ=60)
                                                                                        CGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAAT       <  SRR4302540.5012881/94‑1 (MQ=60)
                                                                                        CGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAAT       >  SRR4302540.9246477/1‑94 (MQ=60)
                                                                                        CGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAAT       >  SRR4302540.8253633/1‑94 (MQ=60)
                                                                                        CGGCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTCTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGTAT       >  SRR4302540.9146396/1‑94 (MQ=60)
                                                                                          GCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATAT     <  SRR4302540.1183350/94‑1 (MQ=60)
                                                                                          GCCCGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATAT     <  SRR4302540.7376213/94‑1 (MQ=60)
                                                                                            CCGGTCGCCTGGGCGATACTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTG   <  SRR4302540.5368862/94‑1 (MQ=60)
                                                                                             CGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGG  <  SRR4302540.2843422/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATTGCTGGCCTTTGCGCTTAATTACATCCCGAATATTGG  >  NC_000913/1672469‑1672655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑